pycom16g20890

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0015199
Physical Location & Seq
Forward (+)
18968852 .. 18972473
3622 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 885 bp
ATGGCGGAAGAACCCAAAACGTTGTCGTCCAGCTTGCAACCTTCTTCTACACCGAATTCCCCCGAAGAAACTCTGAAGAAATCGCAGGTTCCAGCACTCTCTGGGCTTCCATTTTCCCCAAATGGTGATTTTCTGATGTTACCAGTCATGTATCCTCTTCTCGTTCCTGGGTTAAATCCTTCGCAAGATCAGGAGCAAATGAACCGTGGAGCAGGCATTTATGCTGTTCCTGTTCACCCATTTATGGGGCCAGTTACAGGAGCTCCATTAAACACTCTTATTCCTCTTACCTACAACATACCCACAGCTAGAGCAAGTCCAGAGGTTGGCACTGTAGGGGAGCAGCAAGGCCAAGAGGGACAACAACAACAACAACGTCAACAGCCTGGACCTCAGAGACAAGTTGTTGGTAGAAGATTTCAGATTGCATTTCAGCTTGATGTGTTGCTTATTATAAAGCTTGCAGCTGTAATATTTCTGTTCAACCAAGATGGATCTCGACAAAGGCTGGTTCTCCTTGTGATTTTCGCTTCAATTGTCTATTTATATCAAACTGGCTCGCTCACACCATTAGTACGATGGCTTTCTCAAGGCATGCATAGGGCAGCTGTGCCCCCCCATCCGCCCAGGGCTGCCAGGGCCGGGAATGTTCCTCCTAATGCAAGGCAGGGGGTTGACAATGCTGCTTTGGCAGATGGACAGCCTGGGGCTGGGAACGATATTCGGCCTGCAGATGAAGGAAATCCAGCAGCAGAGAATGAGAATGCCCCGGCAGCTGATGGAGCCAATGGTGGTCACCAGTGGTGGGGAATTGTTAAAGAGATTCAGATGATCGTTTTCGGTTTCATCACTTCACTTCTTCCAGGTTTTCATAATATGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

295

Amino Acids

31.59

Weight (kDa)

5.96

Isoelectric Point (pI)

53.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015199)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G29960
fragaria_vesca FvH4_4g07340
malus_domestica MD16G1249500.v1.1
prunus_persica Prupe.1G076300_v2.0.a1 Prupe.1G076300_v2.0.a1
pyrus_communis pycom16g20890
rosa_chinensis RchiOBHm_Chr4g0399711
rosa_laevigata RLG00000009245
rosa_multiflora Rmu_ssc0000118.1_g000010
rosa_roxburghii Rroxscaffold_5G00344650
rosa_rugosa Rorug04G0018500
rosa_samantha Rh4AG096000 Rh4BG093500 Rh4CG104800 Rh4DG088800
rosa_wichuraiana Rw4G007930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 455
Acc36I ACCTGC 1 cut(s) 76
AccB7I CCANNNNNTGG 1 cut(s) 326
AciI CCGC 2 cut(s) 5, 623
AclI AACGTT 1 cut(s) 20
AclWI GGATC 1 cut(s) 502
AcsI RAATTY 1 cut(s) 55
AcuI CTGAAG 1 cut(s) 95
AfaI GTAC 1 cut(s) 576
AfiI CCNNNNNNNGG 7 cut(s) 244, 245, 257, 326, 642, 710, 805
AgsI TTSAA 2 cut(s) 484, 534
AjnI CCWGG 6 cut(s) 166, 385, 626, 635, 703, 862
AluBI AGCT 8 cut(s) 33, 263, 308, 436, 460, 467, 608, 776
AluI AGCT 8 cut(s) 33, 263, 308, 436, 460, 467, 608, 776
Alw21I GWGCWC 1 cut(s) 265
Alw26I GTCTC 1 cut(s) 391
AlwI GGATC 1 cut(s) 502
AoxI GGCC 4 cut(s) 248, 349, 639, 725
ApeKI GCWGC 7 cut(s) 343, 464, 605, 632, 683, 749, 773
ApoI RAATTY 1 cut(s) 55
AspS9I GGNCC 3 cut(s) 248, 389, 639
AsuC2I CCSGG 2 cut(s) 643, 770
AsuHPI GGTGA 3 cut(s) 137, 227, 788
AvaII GGWCC 1 cut(s) 389
BaeGI GKGCMC 1 cut(s) 615
BanII GRGCYC 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 265
BbvI GCAGC 7 cut(s) 355, 476, 617, 619, 670, 761, 785
BccI CCATC 5 cut(s) 485, 573, 627, 689, 773
BciT130I CCWGG 6 cut(s) 168, 387, 628, 637, 705, 864
BciVI GTATCC 1 cut(s) 162
BcnI CCSGG 2 cut(s) 643, 770
BcoDI GTCTC 1 cut(s) 391
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 2 cut(s) 333, 729
BfuAI ACCTGC 1 cut(s) 76
BfuI GTATCC 1 cut(s) 162
BisI GCNGC 7 cut(s) 344, 465, 606, 633, 684, 750, 774
BlsI GCNGC 7 cut(s) 345, 466, 607, 634, 685, 751, 775
Bme1390I CCNGG 8 cut(s) 168, 387, 628, 637, 643, 705, 770, 864
Bme18I GGWCC 1 cut(s) 389
BmgT120I GGNCC 3 cut(s) 248, 389, 639
BmiI GGNNCC 3 cut(s) 90, 249, 784
BmrFI CCNGG 8 cut(s) 168, 387, 628, 637, 643, 705, 770, 864
BpuEI CTTGAG 1 cut(s) 573
BpuMI CCSGG 2 cut(s) 643, 770
BsaJI CCNNGG 7 cut(s) 167, 205, 626, 627, 636, 704, 768
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 7 cut(s) 244, 245, 257, 326, 642, 710, 805
Bse1I ACTGG 4 cut(s) 143, 251, 559, 799
BseBI CCWGG 6 cut(s) 168, 387, 628, 637, 705, 864
BseDI CCNNGG 7 cut(s) 167, 205, 626, 627, 636, 704, 768
BseGI GGATG 1 cut(s) 619
BseLI CCNNNNNNNGG 7 cut(s) 244, 245, 257, 326, 642, 710, 805
BseMII CTCAG 1 cut(s) 407
BseNI ACTGG 4 cut(s) 143, 251, 559, 799
BseSI GKGCMC 1 cut(s) 615
BseXI GCAGC 7 cut(s) 355, 476, 617, 619, 670, 761, 785
BseYI CCCAGC 1 cut(s) 710
BshFI GGCC 4 cut(s) 250, 351, 641, 727
BsiHKAI GWGCWC 1 cut(s) 265
BsiSI CCGG 2 cut(s) 642, 770
BslFI GGGAC 1 cut(s) 372
BslI CCNNNNNNNGG 7 cut(s) 244, 245, 257, 326, 642, 710, 805
BsmAI GTCTC 1 cut(s) 391
BsmFI GGGAC 1 cut(s) 372
BsmI GAATGC 1 cut(s) 769
BsnI GGCC 4 cut(s) 250, 351, 641, 727
Bsp1286I GDGCHC 2 cut(s) 265, 615
Bsp143I GATC 3 cut(s) 187, 494, 831
BspACI CCGC 2 cut(s) 5, 623
BspANI GGCC 4 cut(s) 250, 351, 641, 727
BspCNI CTCAG 1 cut(s) 406
BspLI GGNNCC 3 cut(s) 90, 249, 784
BspMAI CTGCAG 1 cut(s) 733
BspMI ACCTGC 1 cut(s) 76
BspPI GGATC 1 cut(s) 502
BsrI ACTGG 4 cut(s) 143, 251, 559, 799
BssECI CCNNGG 7 cut(s) 167, 205, 626, 627, 636, 704, 768
BssMI GATC 3 cut(s) 187, 494, 831
Bst2UI CCWGG 6 cut(s) 168, 387, 628, 637, 705, 864
Bst4CI ACNGT 2 cut(s) 206, 334
Bst6I CTCTTC 1 cut(s) 162
BstC8I GCNNGC 6 cut(s) 35, 214, 462, 560, 596, 729
BstDEI CTNAG 1 cut(s) 393
BstDSI CCRYGG 1 cut(s) 205
BstEII GGTNACC 1 cut(s) 794
BstF5I GGATG 1 cut(s) 619
BstKTI GATC 3 cut(s) 190, 497, 834
BstMAI GTCTC 1 cut(s) 391
BstMBI GATC 3 cut(s) 187, 494, 831
BstMWI GCNNNNNNNGC 4 cut(s) 638, 689, 773, 782
BstNI CCWGG 6 cut(s) 168, 387, 628, 637, 705, 864
BstNSI RCATGY 1 cut(s) 598
BstPI GGTNACC 1 cut(s) 794
BstSCI CCNGG 8 cut(s) 166, 385, 626, 635, 641, 703, 768, 862
BstSFI CTRYAG 2 cut(s) 333, 729
BstSLI GKGCMC 1 cut(s) 615
BstV1I GCAGC 7 cut(s) 355, 476, 617, 619, 670, 761, 785
BstX2I RGATCY 1 cut(s) 494
BstYI RGATCY 1 cut(s) 494
BsuI GTATCC 1 cut(s) 162
BsuRI GGCC 4 cut(s) 250, 351, 641, 727
BtgI CCRYGG 1 cut(s) 205
BtsCI GGATG 1 cut(s) 619
BtsIMutI CAGTG 2 cut(s) 330, 806
BveI ACCTGC 1 cut(s) 76
Cac8I GCNNGC 6 cut(s) 35, 214, 462, 560, 596, 729
Cfr13I GGNCC 3 cut(s) 248, 389, 639
Csp6I GTAC 1 cut(s) 575
CviAII CATG 2 cut(s) 148, 595
CviQI GTAC 1 cut(s) 575
DdeI CTNAG 1 cut(s) 393
DpnI GATC 3 cut(s) 189, 496, 833
DpnII GATC 3 cut(s) 187, 494, 831
Eam1104I CTCTTC 1 cut(s) 162
EarI CTCTTC 1 cut(s) 162
EciI GGCGGA 2 cut(s) 20, 612
Ecl136II GAGCTC 1 cut(s) 263
Eco24I GRGCYC 1 cut(s) 265
Eco47I GGWCC 1 cut(s) 389
Eco53kI GAGCTC 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 95
Eco91I GGTNACC 1 cut(s) 794
EcoICRI GAGCTC 1 cut(s) 263
EcoO65I GGTNACC 1 cut(s) 794
EcoRI GAATTC 1 cut(s) 55
EcoRII CCWGG 6 cut(s) 166, 385, 626, 635, 703, 862
EcoT22I ATGCAT 1 cut(s) 600
EcoT38I GRGCYC 1 cut(s) 265
FaeI CATG 2 cut(s) 151, 598
FaqI GGGAC 1 cut(s) 372
FatI CATG 2 cut(s) 147, 594
Fnu4HI GCNGC 7 cut(s) 344, 465, 606, 633, 684, 750, 774
FokI GGATG 1 cut(s) 606
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 7 cut(s) 344, 465, 606, 633, 684, 750, 774
FspBI CTAG 1 cut(s) 309
GluI GCNGC 7 cut(s) 344, 465, 606, 633, 684, 750, 774
GsaI CCCAGC 1 cut(s) 714
HaeIII GGCC 4 cut(s) 250, 351, 641, 727
HapII CCGG 2 cut(s) 642, 770
Hin1II CATG 2 cut(s) 151, 598
HincII GTYRAC 2 cut(s) 380, 676
HindII GTYRAC 2 cut(s) 380, 676
HindIII AAGCTT 1 cut(s) 458
HinfI GANTC 1 cut(s) 823
HpaII CCGG 2 cut(s) 642, 770
HphI GGTGA 3 cut(s) 137, 227, 788
Hpy166II GTNNAC 3 cut(s) 235, 380, 676
Hpy188I TCNGA 5 cut(s) 75, 135, 396, 423, 828
Hpy188III TCNNGA 3 cut(s) 191, 320, 498
Hpy8I GTNNAC 3 cut(s) 235, 380, 676
HpyAV CCTTC 3 cut(s) 51, 189, 731
HpyCH4III ACNGT 2 cut(s) 206, 334
HpyCH4IV ACGT 2 cut(s) 20, 376
HpyCH4V TGCA 6 cut(s) 37, 428, 464, 598, 662, 731
HpyF10VI GCNNNNNNNGC 4 cut(s) 638, 689, 773, 782
HpyF3I CTNAG 1 cut(s) 393
HpySE526I ACGT 2 cut(s) 20, 376
Hsp92II CATG 2 cut(s) 151, 598
Kzo9I GATC 3 cut(s) 187, 494, 831
LmnI GCTCC 6 cut(s) 193, 209, 260, 268, 340, 782
Lsp1109I GCAGC 7 cut(s) 355, 476, 617, 619, 670, 761, 785
MaeI CTAG 1 cut(s) 309
MaeII ACGT 2 cut(s) 20, 376
MaeIII GTNAC 3 cut(s) 138, 253, 794
MalI GATC 3 cut(s) 189, 496, 833
MboI GATC 3 cut(s) 187, 494, 831
MboII GAAGA 7 cut(s) 20, 36, 77, 88, 149, 426, 851
MfeI CAATTG 1 cut(s) 534
MflI RGATCY 1 cut(s) 494
MhlI GDGCHC 2 cut(s) 265, 615
MluCI AATT 3 cut(s) 55, 534, 810
MnlI CCTC 6 cut(s) 165, 294, 316, 349, 402, 663
Mph1103I ATGCAT 1 cut(s) 600
MseI TTAA 3 cut(s) 173, 269, 816
MspA1I CMGCKG 3 cut(s) 467, 608, 776
MspI CCGG 2 cut(s) 642, 770
MspR9I CCNGG 8 cut(s) 168, 387, 628, 637, 643, 705, 770, 864
MunI CAATTG 1 cut(s) 534
Mva1269I GAATGC 1 cut(s) 769
MvaI CCWGG 6 cut(s) 168, 387, 628, 637, 705, 864
MwoI GCNNNNNNNGC 4 cut(s) 638, 689, 773, 782
NciI CCSGG 2 cut(s) 643, 770
NdeII GATC 3 cut(s) 187, 494, 831
NlaIII CATG 2 cut(s) 151, 598
NlaIV GGNNCC 3 cut(s) 90, 249, 784
NmuCI GTSAC 1 cut(s) 794
NsiI ATGCAT 1 cut(s) 600
NspI RCATGY 1 cut(s) 598
PaeI GCATGC 1 cut(s) 598
PasI CCCWGGG 1 cut(s) 627
PctI GAATGC 1 cut(s) 769
PfeI GAWTC 1 cut(s) 823
PflMI CCANNNNNTGG 1 cut(s) 326
PkrI GCNGC 7 cut(s) 345, 466, 607, 634, 685, 751, 775
PsiI TTATAA 1 cut(s) 455
Psp124BI GAGCTC 1 cut(s) 265
Psp1406I AACGTT 1 cut(s) 20
Psp6I CCWGG 6 cut(s) 166, 385, 626, 635, 703, 862
PspEI GGTNACC 1 cut(s) 794
PspFI CCCAGC 1 cut(s) 710
PspGI CCWGG 6 cut(s) 166, 385, 626, 635, 703, 862
PspN4I GGNNCC 3 cut(s) 90, 249, 784
PspPI GGNCC 3 cut(s) 248, 389, 639
PstI CTGCAG 1 cut(s) 733
PsuI RGATCY 1 cut(s) 494
PvuII CAGCTG 3 cut(s) 467, 608, 776
RsaI GTAC 1 cut(s) 576
RsaNI GTAC 1 cut(s) 575
SacI GAGCTC 1 cut(s) 265
SaqAI TTAA 3 cut(s) 173, 269, 816
SatI GCNGC 7 cut(s) 344, 465, 606, 633, 684, 750, 774
Sau3AI GATC 3 cut(s) 187, 494, 831
Sau96I GGNCC 3 cut(s) 248, 389, 639
ScrFI CCNGG 8 cut(s) 168, 387, 628, 637, 643, 705, 770, 864
SduI GDGCHC 2 cut(s) 265, 615
SfcI CTRYAG 2 cut(s) 333, 729
SinI GGWCC 1 cut(s) 389
SmlI CTYRAG 1 cut(s) 588
SmoI CTYRAG 1 cut(s) 588
SphI GCATGC 1 cut(s) 598
Sse9I AATT 3 cut(s) 55, 534, 810
SsiI CCGC 2 cut(s) 5, 623
SspI AATATT 1 cut(s) 474
SspMI CTAG 1 cut(s) 309
SstI GAGCTC 1 cut(s) 265
StyD4I CCNGG 8 cut(s) 166, 385, 626, 635, 641, 703, 768, 862
TaaI ACNGT 2 cut(s) 206, 334
TaiI ACGT 2 cut(s) 23, 379
TaqI TCGA 1 cut(s) 499
TasI AATT 3 cut(s) 55, 534, 810
TfiI GAWTC 1 cut(s) 823
Tru1I TTAA 3 cut(s) 173, 269, 816
Tru9I TTAA 3 cut(s) 173, 269, 816
TscAI CASTG 2 cut(s) 337, 806
TseFI GTSAC 1 cut(s) 794
TseI GCWGC 7 cut(s) 343, 464, 605, 632, 683, 749, 773
Tsp45I GTSAC 1 cut(s) 794
TspDTI ATGAA 4 cut(s) 215, 750, 835, 860
TspRI CASTG 2 cut(s) 337, 806
Van91I CCANNNNNTGG 1 cut(s) 326
VpaK11BI GGWCC 1 cut(s) 389
XapI RAATTY 1 cut(s) 55
XceI RCATGY 1 cut(s) 598
XcmI CCANNNNNNNNNTGG 1 cut(s) 576
XspI CTAG 1 cut(s) 309
Zsp2I ATGCAT 1 cut(s) 600
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.