pycom16g21160

2S seed storage protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0017371
Physical Location & Seq
Reverse (-)
19381787 .. 19382554
768 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 768 bp
ATGAAGAAGGAGCTGATTTGGCTTATTTGGATCCCAATTTTTGTCTATCTGTTCATTACAGAGAAAGTGTTGGAATCTAAAGGTCTTCGCCTAAGTCGGTCAAAGACAGAGTATATGGAGTGCAAGTTCAATGCAAACGGAGGCTCAAATGAATTAGGGGTGACGATTGGAGATCAGGAAGTACCAAAGAGGGACTGCTTTCGCTATCTAGTATCTATCTTGGAAAAGAATGGAGAGTTGGATGGAGACCTCAACCATAGAATACAAGTTGGATGGATGAAGTGGAAGAGTGCATCCGGCGTGTTGTGTGACCGTCGTATGCCACTGAAGCTCAAGGGAAAATTTTATAGGATGGCAATAAGGCGGACAATACTTTATGGCATGGAATGTTGGGCGGTGAAGCATCAACACATACATAAAATGGGTGTAGCGGAGATGAGAATGCTTCCTTGGATGTGTGGGCACACGAGAAAGGATAAGATTAGGAATGAGGATATCCGAGGTAAAATAGGAGTAGCCAAAATTGAAGGAAAGAAGATAGATAATTGGTTATGGTGGTTTAGACATGTGAAACGAAGGCCTACTGACGCTCCGGTTAAAAGATACGATTACGGGATAGAGGTTCATGGCCGAAAGGGTAGAGGAAGACCAAGAAGGACCTTGGAAGAGACTCGAAGAAAAGACTTAGAGTGCTTGGATCTAACAGAAGACGTGATACAGAACCGAGCGCAATGGCGTTCTAGGATTCATATAGCCGACCCCACTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RVT_1 Tryp_alpha_amyl

Protein Analysis

256

Amino Acids

30.29

Weight (kDa)

9.91

Isoelectric Point (pI)

58.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 364, 395, 431
AclWI GGATC 3 cut(s) 25, 38, 705
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 1 cut(s) 341
AcuI CTGAAG 1 cut(s) 347
AfaI GTAC 1 cut(s) 183
AflIII ACRYGT 1 cut(s) 565
AgsI TTSAA 2 cut(s) 130, 527
AjiI CACGTC 1 cut(s) 712
AjuI GAANNNNNNNTTGG 4 cut(s) 221, 253, 433, 465
AloI GAACNNNNNNTCC 2 cut(s) 110, 142
AluBI AGCT 2 cut(s) 13, 331
AluI AGCT 2 cut(s) 13, 331
Alw26I GTCTC 2 cut(s) 240, 662
AlwI GGATC 3 cut(s) 25, 38, 705
AoxI GGCC 2 cut(s) 578, 628
ApoI RAATTY 1 cut(s) 341
AspLEI GCGC 1 cut(s) 730
AspS9I GGNCC 1 cut(s) 657
AsuHPI GGTGA 2 cut(s) 172, 409
AvaII GGWCC 1 cut(s) 657
BaeGI GKGCMC 1 cut(s) 465
BamHI GGATCC 1 cut(s) 30
BarI GAAGNNNNNNTAC 2 cut(s) 699, 731
BauI CACGAG 1 cut(s) 466
BbsI GAAGAC 3 cut(s) 77, 652, 714
BccI CCATC 3 cut(s) 236, 267, 346
BcoDI GTCTC 2 cut(s) 240, 662
BfaI CTAG 2 cut(s) 209, 741
Bme18I GGWCC 1 cut(s) 657
BmgBI CACGTC 1 cut(s) 712
BmgT120I GGNCC 1 cut(s) 657
BmiI GGNNCC 1 cut(s) 32
BmsI GCATC 2 cut(s) 302, 412
BpiI GAAGAC 3 cut(s) 77, 652, 714
BpuEI CTTGAG 1 cut(s) 317
BsaI GGTCTC 1 cut(s) 240
BsaJI CCNNGG 3 cut(s) 449, 499, 660
BsaWI WCCGGW 1 cut(s) 592
BsaXI ACNNNNNCTCC 4 cut(s) 110, 140, 574, 604
Bse3DI GCAATG 1 cut(s) 737
BseDI CCNNGG 3 cut(s) 449, 499, 660
BseGI GGATG 6 cut(s) 247, 278, 282, 293, 357, 459
BseMI GCAATG 1 cut(s) 737
BseSI GKGCMC 1 cut(s) 465
BshFI GGCC 2 cut(s) 580, 630
BsiSI CCGG 2 cut(s) 297, 593
BslFI GGGAC 1 cut(s) 206
BsmAI GTCTC 2 cut(s) 240, 662
BsmFI GGGAC 1 cut(s) 206
BsmI GAATGC 1 cut(s) 447
BsnI GGCC 2 cut(s) 580, 630
Bso31I GGTCTC 1 cut(s) 240
Bsp1286I GDGCHC 1 cut(s) 465
Bsp143I GATC 3 cut(s) 30, 172, 697
BspACI CCGC 3 cut(s) 364, 395, 431
BspANI GGCC 2 cut(s) 580, 630
BspLI GGNNCC 1 cut(s) 32
BspPI GGATC 3 cut(s) 25, 38, 705
BspTNI GGTCTC 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 737
BssECI CCNNGG 3 cut(s) 449, 499, 660
BssMI GATC 3 cut(s) 30, 172, 697
BssSI CACGAG 1 cut(s) 466
BssT1I CCWWGG 2 cut(s) 449, 660
Bst2BI CACGAG 1 cut(s) 466
Bst4CI ACNGT 1 cut(s) 314
Bst6I CTCTTC 2 cut(s) 281, 660
BstDEI CTNAG 3 cut(s) 92, 685, 765
BstF5I GGATG 6 cut(s) 247, 278, 282, 293, 357, 459
BstHHI GCGC 1 cut(s) 730
BstKTI GATC 3 cut(s) 33, 175, 700
BstMAI GTCTC 2 cut(s) 240, 662
BstMBI GATC 3 cut(s) 30, 172, 697
BstMWI GCNNNNNNNGC 2 cut(s) 19, 328
BstNSI RCATGY 1 cut(s) 569
BstSLI GKGCMC 1 cut(s) 465
BstV2I GAAGAC 3 cut(s) 77, 652, 714
BstX2I RGATCY 2 cut(s) 30, 697
BstYI RGATCY 2 cut(s) 30, 697
BsuRI GGCC 2 cut(s) 580, 630
BtrI CACGTC 1 cut(s) 712
BtsCI GGATG 6 cut(s) 247, 278, 282, 293, 357, 459
BtsIMutI CAGTG 1 cut(s) 323
CfoI GCGC 1 cut(s) 730
Cfr13I GGNCC 1 cut(s) 657
CseI GACGC 1 cut(s) 596
Csp6I GTAC 1 cut(s) 182
CviAII CATG 3 cut(s) 382, 566, 626
CviJI RGCY 8 cut(s) 13, 22, 144, 331, 518, 580, 630, 755
CviKI_1 RGCY 8 cut(s) 13, 22, 144, 331, 518, 580, 630, 755
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 3 cut(s) 92, 685, 765
DpnI GATC 3 cut(s) 32, 174, 699
DpnII GATC 3 cut(s) 30, 172, 697
EaeI YGGCCR 1 cut(s) 628
Eam1104I CTCTTC 2 cut(s) 281, 660
EarI CTCTTC 2 cut(s) 281, 660
EciI GGCGGA 1 cut(s) 379
Eco130I CCWWGG 2 cut(s) 449, 660
Eco147I AGGCCT 1 cut(s) 580
Eco31I GGTCTC 1 cut(s) 240
Eco32I GATATC 1 cut(s) 496
Eco47I GGWCC 1 cut(s) 657
Eco57I CTGAAG 1 cut(s) 347
EcoO109I RGGNCCY 1 cut(s) 657
EcoRV GATATC 1 cut(s) 496
EcoT14I CCWWGG 2 cut(s) 449, 660
ErhI CCWWGG 2 cut(s) 449, 660
FaeI CATG 3 cut(s) 385, 569, 629
FaqI GGGAC 1 cut(s) 206
FatI CATG 3 cut(s) 381, 565, 625
FokI GGATG 6 cut(s) 254, 280, 285, 289, 364, 466
FspBI CTAG 2 cut(s) 209, 741
GlaI GCGC 1 cut(s) 729
HaeIII GGCC 2 cut(s) 580, 630
HapII CCGG 2 cut(s) 297, 593
HgaI GACGC 1 cut(s) 596
HhaI GCGC 1 cut(s) 730
Hin1II CATG 3 cut(s) 385, 569, 629
Hin6I GCGC 1 cut(s) 728
HinP1I GCGC 1 cut(s) 728
HinfI GANTC 3 cut(s) 74, 670, 745
HpaII CCGG 2 cut(s) 297, 593
HphI GGTGA 2 cut(s) 172, 409
Hpy188I TCNGA 1 cut(s) 500
Hpy188III TCNNGA 1 cut(s) 176
Hpy99I CGWCG 1 cut(s) 318
HpyAV CCTTC 3 cut(s) 521, 570, 648
HpyCH4III ACNGT 1 cut(s) 314
HpyCH4IV ACGT 1 cut(s) 711
HpyCH4V TGCA 3 cut(s) 123, 134, 293
HpyF10VI GCNNNNNNNGC 2 cut(s) 19, 328
HpyF3I CTNAG 3 cut(s) 92, 685, 765
HpySE526I ACGT 1 cut(s) 711
Hsp92II CATG 3 cut(s) 385, 569, 629
HspAI GCGC 1 cut(s) 728
Kzo9I GATC 3 cut(s) 30, 172, 697
LmnI GCTCC 2 cut(s) 10, 595
LpnPI CCDG 3 cut(s) 161, 310, 606
LweI GCATC 2 cut(s) 302, 412
MaeI CTAG 2 cut(s) 209, 741
MaeII ACGT 1 cut(s) 711
MaeIII GTNAC 2 cut(s) 160, 308
MalI GATC 3 cut(s) 32, 174, 699
MboI GATC 3 cut(s) 30, 172, 697
MboII GAAGA 8 cut(s) 16, 77, 298, 547, 657, 677, 687, 719
MflI RGATCY 2 cut(s) 30, 697
MhlI GDGCHC 1 cut(s) 465
MluCI AATT 5 cut(s) 36, 152, 341, 522, 544
MlyI GAGTC 1 cut(s) 664
MmeI TCCRAC 3 cut(s) 51, 219, 250
MnlI CCTC 7 cut(s) 134, 183, 260, 484, 494, 613, 635
MseI TTAA 1 cut(s) 597
MspI CCGG 2 cut(s) 297, 593
Mva1269I GAATGC 1 cut(s) 447
MwoI GCNNNNNNNGC 2 cut(s) 19, 328
NdeII GATC 3 cut(s) 30, 172, 697
NlaIII CATG 3 cut(s) 385, 569, 629
NlaIV GGNNCC 1 cut(s) 32
NmuCI GTSAC 2 cut(s) 160, 308
NspI RCATGY 1 cut(s) 569
PceI AGGCCT 1 cut(s) 580
PciI ACATGT 1 cut(s) 565
PctI GAATGC 1 cut(s) 447
PfeI GAWTC 2 cut(s) 74, 745
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
PpuMI RGGWCCY 1 cut(s) 657
PscI ACATGT 1 cut(s) 565
Psp5II RGGWCCY 1 cut(s) 657
PspN4I GGNNCC 1 cut(s) 32
PspPI GGNCC 1 cut(s) 657
PspPPI RGGWCCY 1 cut(s) 657
PsuI RGATCY 2 cut(s) 30, 697
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SaqAI TTAA 1 cut(s) 597
Sau3AI GATC 3 cut(s) 30, 172, 697
Sau96I GGNCC 1 cut(s) 657
SchI GAGTC 1 cut(s) 664
SduI GDGCHC 1 cut(s) 465
SetI ASST 8 cut(s) 15, 85, 252, 333, 505, 624, 662, 714
SfaNI GCATC 2 cut(s) 302, 412
SinI GGWCC 1 cut(s) 657
SmlI CTYRAG 1 cut(s) 332
SmoI CTYRAG 1 cut(s) 332
Sse9I AATT 5 cut(s) 36, 152, 341, 522, 544
SseBI AGGCCT 1 cut(s) 580
SsiI CCGC 3 cut(s) 364, 395, 431
SspMI CTAG 2 cut(s) 209, 741
StuI AGGCCT 1 cut(s) 580
StyI CCWWGG 2 cut(s) 449, 660
TaaI ACNGT 1 cut(s) 314
TaiI ACGT 1 cut(s) 714
TaqI TCGA 1 cut(s) 673
TaqII GACCGA 1 cut(s) 87
TasI AATT 5 cut(s) 36, 152, 341, 522, 544
TfiI GAWTC 2 cut(s) 74, 745
Tru1I TTAA 1 cut(s) 597
Tru9I TTAA 1 cut(s) 597
TscAI CASTG 1 cut(s) 330
TseFI GTSAC 2 cut(s) 160, 308
Tsp45I GTSAC 2 cut(s) 160, 308
TspDTI ATGAA 6 cut(s) 17, 43, 165, 293, 614, 737
TspGWI ACGGA 1 cut(s) 153
TspRI CASTG 1 cut(s) 330
VpaK11BI GGWCC 1 cut(s) 657
XapI RAATTY 1 cut(s) 341
XceI RCATGY 1 cut(s) 569
XspI CTAG 2 cut(s) 209, 741
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.