pycom16g21510

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010050
Physical Location & Seq
Reverse (-)
20078703 .. 20079847
1145 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g21510.1

Sequence Viewer

Length: 876 bp
ATGGGAACAAAAATTGAATATATGATCAATATATTAGGAAGCTCACAAAATAGCAGCAGCTTAAAGGTGCATCGGGTGGATGACTGGGACTATTTTCAGAAAACTGGGCTGAAGGAGAAGTATCAGAAGGCTAGAGTTGAGGACGCACTTTGGAACTTGACGGACAAGATGCTTGACAAGCACAGCATGGATACCATCAAGAAGACAATGCAGATGCATGAATCTACCTTTAAACACCAGATTCGAGAGCTACACCGTCTGTACAGTGTACAAAAGATGCTAATGGAAGAACTGAAGAAAGAGATTAAACAAAATAGACTTTGGGGTCCAATAACTAGTAGTGCAGATCACATTAACCAATCCCAGTTGATTAACTGGCAGCACTCGATAACTCAAACTTCTTCTGGATATGATTTCCACCTTCAGAGATTGAGGGATGATCCAAACTCTAGAGAGCGCAGTGGAAGTTGCTCAGGAGACATTCAGAGAGTGTCAAGGGGATTTGACCTTGAATGGCCTGCCGAGGAAGACATCTCAACAAGATTCAGTACAATTGACCAAGAACAAGCAGGACCAAGCTCTCACATGTCCCTCAAAAGTATAAGTGCAGCTGGTTGTGATGAAGATGGTGAAGTGGAGCTTTCATTAAGCATCGGAAGCAACAAGAACAGGAAGAGATCGAAACCTTACCAGCCTCAGTTAGGATGTTCAGAACTGATCCAGAAAGCAAAAGAACTCGATATGCCGGCCTCATTCAAATCAGATAGAGGAGGAGATTGCAGTTATCCGACAACTCCCATGAGCAGCTCTAGTGCAACATGTGATCAAGAAAGAAAGCAGCCACGTTGGCTCGTCCATGGTTTAAAGCTCAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

292

Amino Acids

33.35

Weight (kDa)

8.18

Isoelectric Point (pI)

53.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 324
AclWI GGATC 2 cut(s) 434, 712
AcuI CTGAAG 3 cut(s) 131, 314, 407
AfaI GTAC 3 cut(s) 263, 270, 550
AfiI CCNNNNNNNGG 1 cut(s) 701
AflIII ACRYGT 2 cut(s) 585, 818
AgsI TTSAA 3 cut(s) 17, 512, 757
AhlI ACTAGT 1 cut(s) 335
AluBI AGCT 8 cut(s) 42, 60, 250, 579, 611, 640, 807, 868
AluI AGCT 8 cut(s) 42, 60, 250, 579, 611, 640, 807, 868
Alw26I GTCTC 1 cut(s) 471
AlwI GGATC 2 cut(s) 434, 712
AoxI GGCC 2 cut(s) 515, 747
ApeKI GCWGC 6 cut(s) 54, 57, 379, 608, 804, 838
AspLEI GCGC 1 cut(s) 459
AspS9I GGNCC 2 cut(s) 326, 572
AsuHPI GGTGA 1 cut(s) 641
AvaII GGWCC 2 cut(s) 326, 572
BbsI GAAGAC 2 cut(s) 209, 534
BbvI GCAGC 6 cut(s) 66, 69, 391, 620, 816, 850
BccI CCATC 2 cut(s) 203, 620
BciVI GTATCC 1 cut(s) 184
BclI TGATCA 2 cut(s) 24, 823
BcoDI GTCTC 1 cut(s) 471
BcuI ACTAGT 1 cut(s) 335
BfaI CTAG 4 cut(s) 132, 336, 450, 810
BfuI GTATCC 1 cut(s) 184
BglI GCCNNNNNGGC 1 cut(s) 847
BisI GCNGC 6 cut(s) 55, 58, 380, 609, 805, 839
BlsI GCNGC 6 cut(s) 56, 59, 381, 610, 806, 840
Bme18I GGWCC 2 cut(s) 326, 572
BmgT120I GGNCC 2 cut(s) 326, 572
BmiI GGNNCC 1 cut(s) 327
BmrI ACTGGG 3 cut(s) 94, 114, 358
BmsI GCATC 5 cut(s) 79, 159, 204, 267, 660
BmuI ACTGGG 3 cut(s) 94, 114, 358
BpiI GAAGAC 2 cut(s) 209, 534
Bpu10I CCTNAGC 1 cut(s) 472
BsaJI CCNNGG 2 cut(s) 522, 856
Bsc4I CCNNNNNNNGG 1 cut(s) 701
Bse118I RCCGGY 1 cut(s) 745
Bse1I ACTGG 4 cut(s) 89, 109, 364, 380
BseDI CCNNGG 2 cut(s) 522, 856
BseGI GGATG 3 cut(s) 85, 442, 710
BseLI CCNNNNNNNGG 1 cut(s) 701
BseMII CTCAG 2 cut(s) 486, 710
BseNI ACTGG 4 cut(s) 89, 109, 364, 380
BseRI GAGGAG 2 cut(s) 783, 786
BseXI GCAGC 6 cut(s) 66, 69, 391, 620, 816, 850
BsgI GTGCAG 2 cut(s) 363, 627
BshFI GGCC 2 cut(s) 517, 749
BsiSI CCGG 1 cut(s) 746
BslFI GGGAC 2 cut(s) 101, 574
BslI CCNNNNNNNGG 1 cut(s) 701
BsmAI GTCTC 1 cut(s) 471
BsmFI GGGAC 2 cut(s) 101, 574
BsnI GGCC 2 cut(s) 517, 749
Bsp1407I TGTACA 2 cut(s) 261, 268
Bsp143I GATC 6 cut(s) 24, 346, 439, 677, 717, 823
Bsp19I CCATGG 1 cut(s) 856
BspANI GGCC 2 cut(s) 517, 749
BspCNI CTCAG 2 cut(s) 485, 709
BspLI GGNNCC 1 cut(s) 327
BspPI GGATC 2 cut(s) 434, 712
BsrFI RCCGGY 1 cut(s) 745
BsrGI TGTACA 2 cut(s) 261, 268
BsrI ACTGG 4 cut(s) 89, 109, 364, 380
BssAI RCCGGY 1 cut(s) 745
BssECI CCNNGG 2 cut(s) 522, 856
BssMI GATC 6 cut(s) 24, 346, 439, 677, 717, 823
BssT1I CCWWGG 1 cut(s) 856
Bst4CI ACNGT 2 cut(s) 257, 266
Bst6I CTCTTC 1 cut(s) 668
BstAUI TGTACA 2 cut(s) 261, 268
BstC8I GCNNGC 2 cut(s) 519, 747
BstDEI CTNAG 2 cut(s) 472, 696
BstDSI CCRYGG 1 cut(s) 856
BstENI CCTNNNNNAGG 1 cut(s) 699
BstF5I GGATG 3 cut(s) 85, 442, 710
BstHHI GCGC 1 cut(s) 459
BstKTI GATC 6 cut(s) 27, 349, 442, 680, 720, 826
BstMAI GTCTC 1 cut(s) 471
BstMBI GATC 6 cut(s) 24, 346, 439, 677, 717, 823
BstMWI GCNNNNNNNGC 3 cut(s) 178, 657, 847
BstNSI RCATGY 2 cut(s) 589, 822
BstV1I GCAGC 6 cut(s) 66, 69, 391, 620, 816, 850
BstV2I GAAGAC 2 cut(s) 209, 534
BsuI GTATCC 1 cut(s) 184
BsuRI GGCC 2 cut(s) 517, 749
BtgI CCRYGG 1 cut(s) 856
BtsCI GGATG 3 cut(s) 85, 442, 710
BtsI GCAGTG 1 cut(s) 466
BtsIMutI CAGTG 2 cut(s) 271, 466
Cac8I GCNNGC 2 cut(s) 519, 747
CfoI GCGC 1 cut(s) 459
Cfr10I RCCGGY 1 cut(s) 745
Cfr13I GGNCC 2 cut(s) 326, 572
CseI GACGC 1 cut(s) 152
Csp6I GTAC 3 cut(s) 262, 269, 549
CviAII CATG 6 cut(s) 187, 218, 586, 799, 819, 857
CviQI GTAC 3 cut(s) 262, 269, 549
DdeI CTNAG 2 cut(s) 472, 696
DpnI GATC 6 cut(s) 26, 348, 441, 679, 719, 825
DpnII GATC 6 cut(s) 24, 346, 439, 677, 717, 823
DraI TTTAAA 2 cut(s) 232, 864
DrdI GACNNNNNNGTC 1 cut(s) 324
DseDI GACNNNNNNGTC 1 cut(s) 324
Eam1104I CTCTTC 1 cut(s) 668
EarI CTCTTC 1 cut(s) 668
Eco130I CCWWGG 1 cut(s) 856
Eco47I GGWCC 2 cut(s) 326, 572
Eco57I CTGAAG 3 cut(s) 131, 314, 407
EcoNI CCTNNNNNAGG 1 cut(s) 699
EcoT14I CCWWGG 1 cut(s) 856
EcoT22I ATGCAT 1 cut(s) 219
ErhI CCWWGG 1 cut(s) 856
FaeI CATG 6 cut(s) 190, 221, 589, 802, 822, 860
FalI AAGNNNNNCTT 2 cut(s) 624, 656
FaqI GGGAC 2 cut(s) 101, 574
FatI CATG 6 cut(s) 186, 217, 585, 798, 818, 856
FbaI TGATCA 2 cut(s) 24, 823
Fnu4HI GCNGC 6 cut(s) 55, 58, 380, 609, 805, 839
FokI GGATG 3 cut(s) 92, 449, 717
Fsp4HI GCNGC 6 cut(s) 55, 58, 380, 609, 805, 839
FspBI CTAG 4 cut(s) 132, 336, 450, 810
GlaI GCGC 1 cut(s) 458
GluI GCNGC 6 cut(s) 55, 58, 380, 609, 805, 839
HaeIII GGCC 2 cut(s) 517, 749
HapII CCGG 1 cut(s) 746
HgaI GACGC 1 cut(s) 152
HhaI GCGC 1 cut(s) 459
Hin1II CATG 6 cut(s) 190, 221, 589, 802, 822, 860
Hin6I GCGC 1 cut(s) 457
HinP1I GCGC 1 cut(s) 457
HinfI GANTC 3 cut(s) 221, 241, 543
HpaII CCGG 1 cut(s) 746
HphI GGTGA 1 cut(s) 641
Hpy166II GTNNAC 1 cut(s) 269
Hpy188I TCNGA 8 cut(s) 99, 126, 426, 486, 656, 712, 763, 789
Hpy188III TCNNGA 7 cut(s) 199, 245, 405, 450, 474, 721, 827
Hpy8I GTNNAC 1 cut(s) 269
HpyAV CCTTC 3 cut(s) 106, 121, 431
HpyCH4III ACNGT 2 cut(s) 257, 266
HpyCH4IV ACGT 1 cut(s) 844
HpyCH4V TGCA 7 cut(s) 70, 211, 217, 344, 608, 780, 815
HpyF10VI GCNNNNNNNGC 3 cut(s) 178, 657, 847
HpyF3I CTNAG 2 cut(s) 472, 696
HpySE526I ACGT 1 cut(s) 844
Hsp92II CATG 6 cut(s) 190, 221, 589, 802, 822, 860
HspAI GCGC 1 cut(s) 457
KroI GCCGGC 1 cut(s) 745
KroNI GCCGGC 1 cut(s) 747
Ksp22I TGATCA 2 cut(s) 24, 823
Kzo9I GATC 6 cut(s) 24, 346, 439, 677, 717, 823
LmnI GCTCC 1 cut(s) 637
Lsp1109I GCAGC 6 cut(s) 66, 69, 391, 620, 816, 850
LweI GCATC 5 cut(s) 79, 159, 204, 267, 660
MaeI CTAG 4 cut(s) 132, 336, 450, 810
MaeII ACGT 1 cut(s) 844
MalI GATC 6 cut(s) 26, 348, 441, 679, 719, 825
MboI GATC 6 cut(s) 24, 346, 439, 677, 717, 823
MboII GAAGA 7 cut(s) 214, 299, 307, 393, 539, 635, 685
MfeI CAATTG 1 cut(s) 552
MluCI AATT 2 cut(s) 12, 552
MmeI TCCRAC 1 cut(s) 812
MnlI CCTC 8 cut(s) 133, 426, 517, 602, 705, 760, 761, 764
Mph1103I ATGCAT 1 cut(s) 219
MroNI GCCGGC 1 cut(s) 745
MseI TTAA 7 cut(s) 62, 231, 306, 354, 372, 647, 863
MspA1I CMGCKG 1 cut(s) 611
MspI CCGG 1 cut(s) 746
MunI CAATTG 1 cut(s) 552
MwoI GCNNNNNNNGC 3 cut(s) 178, 657, 847
NaeI GCCGGC 1 cut(s) 747
NcoI CCATGG 1 cut(s) 856
NdeII GATC 6 cut(s) 24, 346, 439, 677, 717, 823
NgoMIV GCCGGC 1 cut(s) 745
NlaIII CATG 6 cut(s) 190, 221, 589, 802, 822, 860
NlaIV GGNNCC 1 cut(s) 327
NmeAIII GCCGAG 1 cut(s) 547
NsiI ATGCAT 1 cut(s) 219
NspI RCATGY 2 cut(s) 589, 822
PciI ACATGT 2 cut(s) 585, 818
PdiI GCCGGC 1 cut(s) 747
PfeI GAWTC 3 cut(s) 221, 241, 543
PkrI GCNGC 6 cut(s) 56, 59, 381, 610, 806, 840
PscI ACATGT 2 cut(s) 585, 818
PspN4I GGNNCC 1 cut(s) 327
PspPI GGNCC 2 cut(s) 326, 572
PvuII CAGCTG 1 cut(s) 611
RsaI GTAC 3 cut(s) 263, 270, 550
RsaNI GTAC 3 cut(s) 262, 269, 549
SaqAI TTAA 7 cut(s) 62, 231, 306, 354, 372, 647, 863
SatI GCNGC 6 cut(s) 55, 58, 380, 609, 805, 839
Sau3AI GATC 6 cut(s) 24, 346, 439, 677, 717, 823
Sau96I GGNCC 2 cut(s) 326, 572
SfaNI GCATC 5 cut(s) 79, 159, 204, 267, 660
SinI GGWCC 2 cut(s) 326, 572
SpeI ACTAGT 1 cut(s) 335
Sse9I AATT 2 cut(s) 12, 552
SspMI CTAG 4 cut(s) 132, 336, 450, 810
StyI CCWWGG 1 cut(s) 856
TaaI ACNGT 2 cut(s) 257, 266
TaiI ACGT 1 cut(s) 847
TaqI TCGA 4 cut(s) 244, 386, 680, 738
TasI AATT 2 cut(s) 12, 552
TatI WGTACW 3 cut(s) 261, 268, 548
TfiI GAWTC 3 cut(s) 221, 241, 543
Tru1I TTAA 7 cut(s) 62, 231, 306, 354, 372, 647, 863
Tru9I TTAA 7 cut(s) 62, 231, 306, 354, 372, 647, 863
TscAI CASTG 2 cut(s) 271, 466
TseI GCWGC 6 cut(s) 54, 57, 379, 608, 804, 838
TspDTI ATGAA 3 cut(s) 234, 633, 636
TspGWI ACGGA 1 cut(s) 176
TspRI CASTG 2 cut(s) 271, 466
VpaK11BI GGWCC 2 cut(s) 326, 572
XagI CCTNNNNNAGG 1 cut(s) 699
XbaI TCTAGA 1 cut(s) 449
XceI RCATGY 2 cut(s) 589, 822
XspI CTAG 4 cut(s) 132, 336, 450, 810
Zsp2I ATGCAT 1 cut(s) 219
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.