pycom16g22650

glutamine amidotransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0012238
Physical Location & Seq
Reverse (-)
22901763 .. 22902694
932 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 765 bp
ATGAAGGTTGAGAAGGAGAGTAGATCTGGATCATATGCACTTCTGCTAGCAGCAAGGGACTCAGAGTATGTGAAGAAAGTGTACGGTGGGTATGTCAATGTGTTTGTTGCAGCGTTTGGGGAAGAAGGAGAGAGGTGGGACTTGTACAGGGTTGTAGATGGAGAGTTTCCTGACATGGATGACCTTCAAAGCTATGATGGCTTCGTCATCAGTGGCAGCCCTTATGATGCTTATGGCAATGACTACTGGATCATCAAACTATGCTTTCTCTTGCAAACTTTGGATGCCATGGAGAAGAAAGTCCTCGGAATTTGCTTTGGTCATCAGGTGTTGTGCAGAGCACTAGGAGGAAAGGTTGGGAAAGCTTATACAGGGTGGGATATTGGGCTGAGGAAAGTGAAGCTAGTGAAGGAATTGGCTCCATTCGGCTTCCTGAATGACTTGGATGACGTTCCCCTTCCGCTTGCCCTTTCCATTATTGAGTGCCATCAAGATGAGGTTTGGGAAGTTCCTTTGGGTGCAGAGGTGATCGGGTACTCGGACAAAACAGGTGTGGAGATGTTCACCATTGGAAGCCATGTTCTGGGCATTCAAGGTCATCCTGAATACAGTAAGGACATTCTTTCAAATCTCATTGATCGCCTTTTCAACAGCAACTGCATAGAACTAGGTTTTGCAGAAAACGCAAGGTCTGCACTGCAATTAGCTGAACCAGATAGGAAGTGCTGGGAGAAGTTCTGCAAGACATTTCTCAAGGGAAAATAG

Protein Analysis

255

Amino Acids

28.46

Weight (kDa)

4.98

Isoelectric Point (pI)

34.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GATase PF00117 35 - 205 1.9e-12 Glutamine amidotransferase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012238)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23970 AT4G30550
fragaria_vesca FvH4_7g12720 FvH4_7g12720
malus_domestica MD13G1275500.v1.1 MD16G1259000.v1.1
prunus_persica Prupe.1G101400_v2.0.a1
pyrus_communis pycom16g22650
rosa_chinensis RchiOBHm_Chr4g0405351
rosa_laevigata RLG00000008849
rosa_multiflora Rmu_sc0000161.1_g000077
rosa_roxburghii Rroxscaffold_5G00349780
rosa_rugosa Rorug04G0057600
rosa_samantha Rh4AG131100 Rh4BG125700 Rh4CG137800 Rh4CG138000 Rh4DG125100
rosa_wichuraiana Rw4G010620

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 583
AciI CCGC 1 cut(s) 461
AclWI GGATC 2 cut(s) 37, 257
AcsI RAATTY 1 cut(s) 309
AfaI GTAC 3 cut(s) 83, 146, 536
AfiI CCNNNNNNNGG 1 cut(s) 583
AgsI TTSAA 4 cut(s) 188, 593, 627, 649
AloI GAACNNNNNNTCC 2 cut(s) 564, 596
AluBI AGCT 4 cut(s) 192, 365, 403, 707
AluI AGCT 4 cut(s) 192, 365, 403, 707
Alw21I GWGCWC 1 cut(s) 343
AlwI GGATC 2 cut(s) 37, 257
AlwNI CAGNNNCTG 1 cut(s) 657
ApeKI GCWGC 3 cut(s) 50, 110, 216
ApoI RAATTY 1 cut(s) 309
AsuHPI GGTGA 2 cut(s) 538, 556
AsuNHI GCTAGC 1 cut(s) 46
BarI GAAGNNNNNNTAC 2 cut(s) 65, 97
Bbv12I GWGCWC 1 cut(s) 343
BbvCI CCTCAGC 1 cut(s) 389
BbvI GCAGC 3 cut(s) 62, 122, 228
BccI CCATC 3 cut(s) 152, 191, 495
BfaI CTAG 4 cut(s) 47, 344, 404, 668
BglII AGATCT 1 cut(s) 23
BisI GCNGC 3 cut(s) 51, 111, 217
BlsI GCNGC 3 cut(s) 52, 112, 218
BmiI GGNNCC 1 cut(s) 420
BmsI GCATC 2 cut(s) 217, 274
BmtI GCTAGC 1 cut(s) 50
Bpu10I CCTNAGC 1 cut(s) 389
BpuEI CTTGAG 1 cut(s) 737
BsaBI GATNNNNATC 1 cut(s) 28
BsaJI CCNNGG 2 cut(s) 288, 304
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 1 cut(s) 583
Bse1I ACTGG 1 cut(s) 251
Bse3DI GCAATG 1 cut(s) 244
Bse8I GATNNNNATC 1 cut(s) 28
BseDI CCNNGG 2 cut(s) 288, 304
BseGI GGATG 4 cut(s) 184, 289, 451, 598
BseJI GATNNNNATC 1 cut(s) 28
BseLI CCNNNNNNNGG 1 cut(s) 583
BseMI GCAATG 1 cut(s) 244
BseMII CTCAG 2 cut(s) 75, 380
BseNI ACTGG 1 cut(s) 251
BseXI GCAGC 3 cut(s) 62, 122, 228
BseYI CCCAGC 1 cut(s) 726
BsgI GTGCAG 3 cut(s) 355, 540, 678
BsiHKAI GWGCWC 1 cut(s) 343
BslFI GGGAC 2 cut(s) 71, 152
BslI CCNNNNNNNGG 1 cut(s) 583
BsmFI GGGAC 2 cut(s) 71, 152
BsmI GAATGC 1 cut(s) 588
Bsp1286I GDGCHC 1 cut(s) 343
Bsp1407I TGTACA 1 cut(s) 144
Bsp143I GATC 5 cut(s) 23, 29, 249, 528, 637
Bsp19I CCATGG 1 cut(s) 288
BspACI CCGC 1 cut(s) 461
BspCNI CTCAG 2 cut(s) 74, 381
BspLI GGNNCC 1 cut(s) 420
BspOI GCTAGC 1 cut(s) 50
BspPI GGATC 2 cut(s) 37, 257
BsrDI GCAATG 1 cut(s) 244
BsrGI TGTACA 1 cut(s) 144
BsrI ACTGG 1 cut(s) 251
BssECI CCNNGG 2 cut(s) 288, 304
BssMI GATC 5 cut(s) 23, 29, 249, 528, 637
BssT1I CCWWGG 1 cut(s) 288
Bst4CI ACNGT 2 cut(s) 86, 611
BstAPI GCANNNNNTGC 1 cut(s) 692
BstAUI TGTACA 1 cut(s) 144
BstC8I GCNNGC 2 cut(s) 48, 465
BstDEI CTNAG 2 cut(s) 61, 389
BstDSI CCRYGG 1 cut(s) 288
BstF5I GGATG 4 cut(s) 184, 289, 451, 598
BstKTI GATC 5 cut(s) 26, 32, 252, 531, 640
BstMBI GATC 5 cut(s) 23, 29, 249, 528, 637
BstMWI GCNNNNNNNGC 3 cut(s) 198, 683, 692
BstV1I GCAGC 3 cut(s) 62, 122, 228
BstX2I RGATCY 1 cut(s) 23
BstYI RGATCY 1 cut(s) 23
BtgI CCRYGG 1 cut(s) 288
BtsCI GGATG 4 cut(s) 184, 289, 451, 598
BtsI GCAGTG 1 cut(s) 695
BtsIMutI CAGTG 2 cut(s) 217, 695
Cac8I GCNNGC 2 cut(s) 48, 465
CaiI CAGNNNCTG 1 cut(s) 657
Csp6I GTAC 3 cut(s) 82, 145, 535
CviAII CATG 3 cut(s) 175, 289, 578
CviQI GTAC 3 cut(s) 82, 145, 535
DdeI CTNAG 2 cut(s) 61, 389
DpnI GATC 5 cut(s) 25, 31, 251, 530, 639
DpnII GATC 5 cut(s) 23, 29, 249, 528, 637
Eco130I CCWWGG 1 cut(s) 288
EcoT14I CCWWGG 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 288
FaeI CATG 3 cut(s) 178, 292, 581
FaqI GGGAC 2 cut(s) 71, 152
FatI CATG 3 cut(s) 174, 288, 577
FauNDI CATATG 1 cut(s) 34
Fnu4HI GCNGC 3 cut(s) 51, 111, 217
FokI GGATG 4 cut(s) 191, 296, 458, 585
Fsp4HI GCNGC 3 cut(s) 51, 111, 217
FspBI CTAG 4 cut(s) 47, 344, 404, 668
GluI GCNGC 3 cut(s) 51, 111, 217
GsaI CCCAGC 1 cut(s) 730
Hin1II CATG 3 cut(s) 178, 292, 581
HindIII AAGCTT 1 cut(s) 363
HinfI GANTC 1 cut(s) 59
HphI GGTGA 2 cut(s) 538, 556
Hpy166II GTNNAC 2 cut(s) 82, 564
Hpy188I TCNGA 3 cut(s) 64, 308, 541
Hpy188III TCNNGA 5 cut(s) 27, 170, 433, 491, 602
Hpy8I GTNNAC 2 cut(s) 82, 564
HpyAV CCTTC 5 cut(s) 7, 119, 194, 403, 467
HpyCH4III ACNGT 2 cut(s) 86, 611
HpyCH4IV ACGT 1 cut(s) 450
HpyF10VI GCNNNNNNNGC 3 cut(s) 198, 683, 692
HpyF3I CTNAG 2 cut(s) 61, 389
HpySE526I ACGT 1 cut(s) 450
Hsp92II CATG 3 cut(s) 178, 292, 581
Kzo9I GATC 5 cut(s) 23, 29, 249, 528, 637
LmnI GCTCC 1 cut(s) 424
Lsp1109I GCAGC 3 cut(s) 62, 122, 228
LweI GCATC 2 cut(s) 217, 274
MaeI CTAG 4 cut(s) 47, 344, 404, 668
MaeII ACGT 1 cut(s) 450
MalI GATC 5 cut(s) 25, 31, 251, 530, 639
MboI GATC 5 cut(s) 23, 29, 249, 528, 637
MboII GAAGA 3 cut(s) 85, 134, 307
MflI RGATCY 1 cut(s) 23
MhlI GDGCHC 1 cut(s) 343
MluCI AATT 3 cut(s) 309, 413, 701
MlyI GAGTC 1 cut(s) 53
MnlI CCTC 6 cut(s) 126, 314, 341, 384, 490, 517
MslI CAYNNNNRTG 1 cut(s) 492
Mva1269I GAATGC 1 cut(s) 588
MwoI GCNNNNNNNGC 3 cut(s) 198, 683, 692
NcoI CCATGG 1 cut(s) 288
NdeI CATATG 1 cut(s) 34
NdeII GATC 5 cut(s) 23, 29, 249, 528, 637
NheI GCTAGC 1 cut(s) 46
NlaIII CATG 3 cut(s) 178, 292, 581
NlaIV GGNNCC 1 cut(s) 420
PctI GAATGC 1 cut(s) 588
PflMI CCANNNNNTGG 1 cut(s) 583
PkrI GCNGC 3 cut(s) 52, 112, 218
PleI GAGTC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 53
PspFI CCCAGC 1 cut(s) 726
PspN4I GGNNCC 1 cut(s) 420
PstNI CAGNNNCTG 1 cut(s) 657
PsuI RGATCY 1 cut(s) 23
RsaI GTAC 3 cut(s) 83, 146, 536
RsaNI GTAC 3 cut(s) 82, 145, 535
RseI CAYNNNNRTG 1 cut(s) 492
SatI GCNGC 3 cut(s) 51, 111, 217
Sau3AI GATC 5 cut(s) 23, 29, 249, 528, 637
SchI GAGTC 1 cut(s) 53
SduI GDGCHC 1 cut(s) 343
SfaNI GCATC 2 cut(s) 217, 274
SmiMI CAYNNNNRTG 1 cut(s) 492
SmlI CTYRAG 1 cut(s) 752
SmoI CTYRAG 1 cut(s) 752
Sse9I AATT 3 cut(s) 309, 413, 701
SsiI CCGC 1 cut(s) 461
SspMI CTAG 4 cut(s) 47, 344, 404, 668
StyI CCWWGG 1 cut(s) 288
TaaI ACNGT 2 cut(s) 86, 611
TaiI ACGT 1 cut(s) 453
TasI AATT 3 cut(s) 309, 413, 701
TatI WGTACW 1 cut(s) 144
TscAI CASTG 2 cut(s) 217, 702
TseI GCWGC 3 cut(s) 50, 110, 216
TspDTI ATGAA 1 cut(s) 17
TspRI CASTG 2 cut(s) 217, 702
Van91I CCANNNNNTGG 1 cut(s) 583
XapI RAATTY 1 cut(s) 309
XspI CTAG 4 cut(s) 47, 344, 404, 668
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.