pycom16g22990

Belongs to the tetraspanin (TM4SF) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0015286
Physical Location & Seq
Forward (+)
23479353 .. 23494037
14685 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 858 bp
ATGATAGGGAACTTGATAAGCAACAACCTGGTGGGTCTCCTCAACTTCATAACGTTCCTGCTGTCGATTCCGATCGTGGTGGCCGGAGTATGGCTGAGCAAGCAAGGCAGTACCGAGTGTGAGAAGTTCCTAGACAAGCCCATCATCATCCTCGGCGGATTCCTCATGCTGGTGTCGCTGGCCGGCCTAATTGGCGCCTGCTGCCGCGTCTCGTGGCTGCTGTGGGTCTACCTCCTCGTCATGTTCCTCCTCATCGTGGTCCTCTTCGCCGTCACCATCTTCGCCTTCGCCGTCACCAACAAAGGCGCCGGAAAAGTACTTTCCGGGAAAGGGTACAAGGAGTACAGGCTCGGGGACTACTCGCAGTGGCTGCAGAAGAGGGTTAACAGCACCAAGAACTGGAACAAGATCAAGAGCTGTCTCATTGACAGCAAGGTCTGCTCTAGTTTCAAGGACAAGTATGCCAATGATACACTCCAGACCTTCTATTCTGAGAACCTGTCAGCGCTTCAGGCCGGTTGCTGTAAGCCATCAGATGATTGTGGATTTACTTACGAAAGCCCCATCTCCTGGACCAGCAACGGTACTACCGTTTCCTCCAACCCTGACTGCAGTGCGTGGCAGAATAATGAGAAGGTTCTGTGCTTCAACTGTCAGTCGTGCAAGGCTGGACTGCTGGACAACATAAAAAGTAATTGGAAGAAGACGGCGATTGTCAACATTGTCTTCCTCATCTTCCTGATTGTGGTGTATTCAGTTGGATGCTGCGCGTTTAGGAACAGCAGGAGGGACAATGAATATTGGAAACAGAGTAAATTGAATTCTTTTTGTTTTGTAATTGAAGAGAAGAGTGCCTAA

Protein Analysis

286

Amino Acids

31.82

Weight (kDa)

8.9

Isoelectric Point (pI)

33.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 8 - 257 2.4e-39 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015286)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G23810
fragaria_vesca FvH4_4g10210
malus_domestica MD13G1273700.v1.1 MD16G1261500.v1.1
prunus_persica Prupe.1G098200_v2.0.a1
pyrus_communis pycom13g28840 pycom16g22990
rosa_chinensis RchiOBHm_Chr4g0405031
rosa_laevigata RLG00000008883
rosa_multiflora Rmu_ssc0000127.1_g000047
rosa_roxburghii Rroxscaffold_5G00349210
rosa_rugosa Rorug04G0052400
rosa_samantha Rh4AG127300 Rh4BG121200 Rh4CG135300
rosa_wichuraiana Rw4G010320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 434
AccB1I GGYRCC 2 cut(s) 194, 305
AccB7I CCANNNNNTGG 2 cut(s) 399, 570
AccI GTMKAC 1 cut(s) 228
AccII CGCG 2 cut(s) 207, 770
AciI CCGC 2 cut(s) 156, 205
AclI AACGTT 1 cut(s) 53
AcoI YGGCCR 2 cut(s) 81, 180
AcsI RAATTY 1 cut(s) 820
AcuI CTGAAG 1 cut(s) 494
AcyI GRCGYC 2 cut(s) 195, 306
AfaI GTAC 5 cut(s) 112, 318, 335, 344, 586
AfeI AGCGCT 1 cut(s) 507
AfiI CCNNNNNNNGG 7 cut(s) 90, 169, 256, 330, 399, 570, 745
AgsI TTSAA 4 cut(s) 451, 649, 820, 842
AjnI CCWGG 2 cut(s) 27, 569
AluBI AGCT 1 cut(s) 417
AluI AGCT 1 cut(s) 417
Alw26I GTCTC 3 cut(s) 41, 214, 425
AlwNI CAGNNNCTG 1 cut(s) 370
Ama87I CYCGRG 1 cut(s) 350
Aor51HI AGCGCT 1 cut(s) 507
AoxI GGCC 4 cut(s) 81, 180, 184, 513
ApeKI GCWGC 4 cut(s) 201, 217, 370, 765
ApoI RAATTY 1 cut(s) 820
AspLEI GCGC 4 cut(s) 197, 308, 508, 770
AspS9I GGNCC 2 cut(s) 259, 573
AsuC2I CCSGG 1 cut(s) 325
AsuHPI GGTGA 2 cut(s) 265, 286
AvaI CYCGRG 1 cut(s) 350
AvaII GGWCC 2 cut(s) 259, 573
BaeI ACNNNNGTAYC 2 cut(s) 576, 609
BanI GGYRCC 2 cut(s) 194, 305
BauI CACGAG 1 cut(s) 211
BbsI GAAGAC 2 cut(s) 710, 718
BbvI GCAGC 4 cut(s) 188, 204, 357, 752
BccI CCATC 4 cut(s) 149, 284, 538, 572
BceAI ACGGC 3 cut(s) 254, 275, 723
BciT130I CCWGG 2 cut(s) 29, 571
BcnI CCSGG 1 cut(s) 325
BcoDI GTCTC 3 cut(s) 41, 214, 425
BfaI CTAG 2 cut(s) 131, 444
BfmI CTRYAG 2 cut(s) 371, 610
BfoI RGCGCY 3 cut(s) 198, 309, 509
BglI GCCNNNNNGGC 1 cut(s) 192
BisI GCNGC 5 cut(s) 202, 205, 218, 371, 766
BlpI GCTNAGC 1 cut(s) 95
BlsI GCNGC 5 cut(s) 203, 206, 219, 372, 767
BmcAI AGTACT 1 cut(s) 318
Bme1390I CCNGG 3 cut(s) 29, 325, 571
Bme18I GGWCC 2 cut(s) 259, 573
BmeT110I CYCGRG 1 cut(s) 350
BmgT120I GGNCC 2 cut(s) 259, 573
BmiI GGNNCC 2 cut(s) 196, 307
BmrFI CCNGG 3 cut(s) 29, 325, 571
BmsI GCATC 1 cut(s) 752
BpiI GAAGAC 2 cut(s) 710, 718
BpmI CTGGAG 1 cut(s) 461
Bpu1102I GCTNAGC 1 cut(s) 95
BpuMI CCSGG 1 cut(s) 325
BsaBI GATNNNNATC 1 cut(s) 71
BsaHI GRCGYC 2 cut(s) 195, 306
BsaI GGTCTC 1 cut(s) 41
BsaJI CCNNGG 1 cut(s) 151
Bsc4I CCNNNNNNNGG 7 cut(s) 90, 169, 256, 330, 399, 570, 745
Bse118I RCCGGY 2 cut(s) 182, 515
Bse1I ACTGG 1 cut(s) 404
Bse8I GATNNNNATC 1 cut(s) 71
BseBI CCWGG 2 cut(s) 29, 571
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 2 cut(s) 147, 767
BseJI GATNNNNATC 1 cut(s) 71
BseLI CCNNNNNNNGG 7 cut(s) 90, 169, 256, 330, 399, 570, 745
BseMII CTCAG 2 cut(s) 86, 483
BseNI ACTGG 1 cut(s) 404
BseRI GAGGAG 3 cut(s) 29, 224, 239
BseXI GCAGC 4 cut(s) 188, 204, 357, 752
Bsh1236I CGCG 2 cut(s) 207, 770
Bsh1285I CGRYCG 1 cut(s) 75
BshFI GGCC 4 cut(s) 83, 182, 186, 515
BshNI GGYRCC 2 cut(s) 194, 305
BsiEI CGRYCG 1 cut(s) 75
BsiHKCI CYCGRG 1 cut(s) 350
BsiSI CCGG 5 cut(s) 84, 183, 309, 324, 516
BslFI GGGAC 2 cut(s) 368, 803
BslI CCNNNNNNNGG 7 cut(s) 90, 169, 256, 330, 399, 570, 745
BsmAI GTCTC 3 cut(s) 41, 214, 425
BsmBI CGTCTC 1 cut(s) 214
BsmFI GGGAC 2 cut(s) 368, 803
BsnI GGCC 4 cut(s) 83, 182, 186, 515
Bso31I GGTCTC 1 cut(s) 41
BsoBI CYCGRG 1 cut(s) 350
Bsp143I GATC 2 cut(s) 72, 408
Bsp1720I GCTNAGC 1 cut(s) 95
BspACI CCGC 2 cut(s) 156, 205
BspANI GGCC 4 cut(s) 83, 182, 186, 515
BspCNI CTCAG 2 cut(s) 87, 484
BspFNI CGCG 2 cut(s) 207, 770
BspLI GGNNCC 2 cut(s) 196, 307
BspMAI CTGCAG 2 cut(s) 375, 614
BspT107I GGYRCC 2 cut(s) 194, 305
BspTNI GGTCTC 1 cut(s) 41
BsrFI RCCGGY 2 cut(s) 182, 515
BsrI ACTGG 1 cut(s) 404
BssAI RCCGGY 2 cut(s) 182, 515
BssECI CCNNGG 1 cut(s) 151
BssMI GATC 2 cut(s) 72, 408
BssNI GRCGYC 2 cut(s) 195, 306
BssSI CACGAG 1 cut(s) 211
Bst2BI CACGAG 1 cut(s) 211
Bst2UI CCWGG 2 cut(s) 29, 571
Bst4CI ACNGT 3 cut(s) 584, 592, 653
Bst6I CTCTTC 4 cut(s) 269, 371, 837, 842
BstACI GRCGYC 2 cut(s) 195, 306
BstAPI GCANNNNNTGC 2 cut(s) 370, 438
BstC8I GCNNGC 4 cut(s) 101, 180, 184, 199
BstDEI CTNAG 2 cut(s) 95, 492
BstF5I GGATG 2 cut(s) 147, 767
BstFNI CGCG 2 cut(s) 207, 770
BstH2I RGCGCY 3 cut(s) 198, 309, 509
BstHHI GCGC 4 cut(s) 197, 308, 508, 770
BstKTI GATC 2 cut(s) 75, 411
BstMAI GTCTC 3 cut(s) 41, 214, 425
BstMBI GATC 2 cut(s) 72, 408
BstMCI CGRYCG 1 cut(s) 75
BstMWI GCNNNNNNNGC 8 cut(s) 100, 105, 175, 192, 201, 370, 438, 512
BstNI CCWGG 2 cut(s) 29, 571
BstSCI CCNGG 3 cut(s) 27, 323, 569
BstSFI CTRYAG 2 cut(s) 371, 610
BstUI CGCG 2 cut(s) 207, 770
BstV1I GCAGC 4 cut(s) 188, 204, 357, 752
BstV2I GAAGAC 2 cut(s) 710, 718
BsuRI GGCC 4 cut(s) 83, 182, 186, 515
BtsCI GGATG 2 cut(s) 147, 767
BtsI GCAGTG 2 cut(s) 371, 619
BtsIMutI CAGTG 2 cut(s) 371, 619
Cac8I GCNNGC 4 cut(s) 101, 180, 184, 199
CaiI CAGNNNCTG 1 cut(s) 370
CfoI GCGC 4 cut(s) 197, 308, 508, 770
Cfr10I RCCGGY 2 cut(s) 182, 515
Cfr13I GGNCC 2 cut(s) 259, 573
CseI GACGC 1 cut(s) 196
CsiI ACCWGGT 1 cut(s) 27
Csp6I GTAC 5 cut(s) 111, 317, 334, 343, 585
CviAII CATG 2 cut(s) 166, 241
CviQI GTAC 5 cut(s) 111, 317, 334, 343, 585
DdeI CTNAG 2 cut(s) 95, 492
DinI GGCGCC 2 cut(s) 196, 307
DpnI GATC 2 cut(s) 74, 410
DpnII GATC 2 cut(s) 72, 408
DrdI GACNNNNNNGTC 1 cut(s) 434
DseDI GACNNNNNNGTC 1 cut(s) 434
EaeI YGGCCR 2 cut(s) 81, 180
Eam1104I CTCTTC 4 cut(s) 269, 371, 837, 842
EarI CTCTTC 4 cut(s) 269, 371, 837, 842
EciI GGCGGA 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 41
Eco47I GGWCC 2 cut(s) 259, 573
Eco47III AGCGCT 1 cut(s) 507
Eco57I CTGAAG 1 cut(s) 494
Eco88I CYCGRG 1 cut(s) 350
EcoRI GAATTC 1 cut(s) 820
EcoRII CCWGG 2 cut(s) 27, 569
EgeI GGCGCC 2 cut(s) 196, 307
EheI GGCGCC 2 cut(s) 196, 307
Esp3I CGTCTC 1 cut(s) 214
FaeI CATG 2 cut(s) 169, 244
FaiI YATR 6 cut(s) 50, 91, 167, 242, 462, 686
FaqI GGGAC 2 cut(s) 368, 803
FatI CATG 2 cut(s) 165, 240
FblI GTMKAC 1 cut(s) 228
Fnu4HI GCNGC 5 cut(s) 202, 205, 218, 371, 766
FokI GGATG 2 cut(s) 134, 774
FseI GGCCGGCC 1 cut(s) 186
Fsp4HI GCNGC 5 cut(s) 202, 205, 218, 371, 766
FspBI CTAG 2 cut(s) 131, 444
GlaI GCGC 4 cut(s) 196, 307, 507, 769
GluI GCNGC 5 cut(s) 202, 205, 218, 371, 766
GsuI CTGGAG 1 cut(s) 461
HaeII RGCGCY 3 cut(s) 198, 309, 509
HaeIII GGCC 4 cut(s) 83, 182, 186, 515
HapII CCGG 5 cut(s) 84, 183, 309, 324, 516
HgaI GACGC 1 cut(s) 196
HhaI GCGC 4 cut(s) 197, 308, 508, 770
Hin1I GRCGYC 2 cut(s) 195, 306
Hin1II CATG 2 cut(s) 169, 244
Hin6I GCGC 4 cut(s) 195, 306, 506, 768
HinP1I GCGC 4 cut(s) 195, 306, 506, 768
HincII GTYRAC 2 cut(s) 385, 718
HindII GTYRAC 2 cut(s) 385, 718
HinfI GANTC 2 cut(s) 67, 159
HpaI GTTAAC 1 cut(s) 385
HpaII CCGG 5 cut(s) 84, 183, 309, 324, 516
HphI GGTGA 2 cut(s) 265, 286
Hpy166II GTNNAC 3 cut(s) 229, 385, 718
Hpy188I TCNGA 3 cut(s) 72, 493, 535
Hpy188III TCNNGA 3 cut(s) 412, 478, 739
Hpy8I GTNNAC 3 cut(s) 229, 385, 718
HpyAV CCTTC 3 cut(s) 295, 493, 628
HpyCH4III ACNGT 3 cut(s) 584, 592, 653
HpyCH4IV ACGT 1 cut(s) 53
HpyCH4V TGCA 3 cut(s) 373, 612, 663
HpyF10VI GCNNNNNNNGC 8 cut(s) 100, 105, 175, 192, 201, 370, 438, 512
HpyF3I CTNAG 2 cut(s) 95, 492
HpySE526I ACGT 1 cut(s) 53
Hsp92I GRCGYC 2 cut(s) 195, 306
Hsp92II CATG 2 cut(s) 169, 244
HspAI GCGC 4 cut(s) 195, 306, 506, 768
KasI GGCGCC 2 cut(s) 194, 305
KroI GCCGGC 1 cut(s) 182
KroNI GCCGGC 1 cut(s) 184
KspAI GTTAAC 1 cut(s) 385
Kzo9I GATC 2 cut(s) 72, 408
Lsp1109I GCAGC 4 cut(s) 188, 204, 357, 752
LweI GCATC 1 cut(s) 752
MabI ACCWGGT 1 cut(s) 27
MaeI CTAG 2 cut(s) 131, 444
MaeII ACGT 1 cut(s) 53
MaeIII GTNAC 2 cut(s) 271, 292
MalI GATC 2 cut(s) 74, 410
MboI GATC 2 cut(s) 72, 408
MboII GAAGA 8 cut(s) 256, 271, 388, 712, 715, 718, 727, 854
MluCI AATT 5 cut(s) 189, 694, 815, 820, 837
Mly113I GGCGCC 2 cut(s) 195, 306
MmeI TCCRAC 2 cut(s) 624, 739
MroNI GCCGGC 1 cut(s) 182
MseI TTAA 1 cut(s) 384
MslI CAYNNNNRTG 1 cut(s) 170
MspI CCGG 5 cut(s) 84, 183, 309, 324, 516
MspR9I CCNGG 3 cut(s) 29, 325, 571
MvaI CCWGG 2 cut(s) 29, 571
MvnI CGCG 2 cut(s) 207, 770
MwoI GCNNNNNNNGC 8 cut(s) 100, 105, 175, 192, 201, 370, 438, 512
NaeI GCCGGC 1 cut(s) 184
NarI GGCGCC 2 cut(s) 195, 306
NciI CCSGG 1 cut(s) 325
NdeII GATC 2 cut(s) 72, 408
NgoMIV GCCGGC 1 cut(s) 182
NlaIII CATG 2 cut(s) 169, 244
NlaIV GGNNCC 2 cut(s) 196, 307
NmeAIII GCCGAG 1 cut(s) 132
NmuCI GTSAC 2 cut(s) 271, 292
PcsI WCGNNNNNNNCGW 2 cut(s) 288, 588
PdiI GCCGGC 1 cut(s) 184
PfeI GAWTC 2 cut(s) 67, 159
PflMI CCANNNNNTGG 2 cut(s) 399, 570
PfoI TCCNGGA 2 cut(s) 323, 569
PkrI GCNGC 5 cut(s) 203, 206, 219, 372, 767
Ple19I CGATCG 1 cut(s) 75
PluTI GGCGCC 2 cut(s) 198, 309
Psp1406I AACGTT 1 cut(s) 53
Psp6I CCWGG 2 cut(s) 27, 569
PspGI CCWGG 2 cut(s) 27, 569
PspN4I GGNNCC 2 cut(s) 196, 307
PspPI GGNCC 2 cut(s) 259, 573
PstI CTGCAG 2 cut(s) 375, 614
PstNI CAGNNNCTG 1 cut(s) 370
PvuI CGATCG 1 cut(s) 75
RigI GGCCGGCC 1 cut(s) 186
RsaI GTAC 5 cut(s) 112, 318, 335, 344, 586
RsaNI GTAC 5 cut(s) 111, 317, 334, 343, 585
RseI CAYNNNNRTG 1 cut(s) 170
SaqAI TTAA 1 cut(s) 384
SatI GCNGC 5 cut(s) 202, 205, 218, 371, 766
Sau3AI GATC 2 cut(s) 72, 408
Sau96I GGNCC 2 cut(s) 259, 573
ScaI AGTACT 1 cut(s) 318
ScrFI CCNGG 3 cut(s) 29, 325, 571
SetI ASST 8 cut(s) 30, 56, 234, 419, 438, 485, 501, 639
SexAI ACCWGGT 1 cut(s) 27
SfaNI GCATC 1 cut(s) 752
SfcI CTRYAG 2 cut(s) 371, 610
SfoI GGCGCC 2 cut(s) 196, 307
SinI GGWCC 2 cut(s) 259, 573
SmiMI CAYNNNNRTG 1 cut(s) 170
Sse9I AATT 5 cut(s) 189, 694, 815, 820, 837
SsiI CCGC 2 cut(s) 156, 205
SspDI GGCGCC 2 cut(s) 194, 305
SspI AATATT 1 cut(s) 800
SspMI CTAG 2 cut(s) 131, 444
StyD4I CCNGG 3 cut(s) 27, 323, 569
TaaI ACNGT 3 cut(s) 584, 592, 653
TaiI ACGT 1 cut(s) 56
TaqI TCGA 1 cut(s) 65
TasI AATT 5 cut(s) 189, 694, 815, 820, 837
TatI WGTACW 2 cut(s) 316, 342
TauI GCSGC 1 cut(s) 207
TfiI GAWTC 2 cut(s) 67, 159
Tru1I TTAA 1 cut(s) 384
Tru9I TTAA 1 cut(s) 384
TscAI CASTG 2 cut(s) 371, 619
TseFI GTSAC 2 cut(s) 271, 292
TseI GCWGC 4 cut(s) 201, 217, 370, 765
Tsp45I GTSAC 2 cut(s) 271, 292
TspDTI ATGAA 2 cut(s) 37, 810
TspRI CASTG 2 cut(s) 371, 619
Van91I CCANNNNNTGG 2 cut(s) 399, 570
VpaK11BI GGWCC 2 cut(s) 259, 573
XapI RAATTY 1 cut(s) 820
XmiI GTMKAC 1 cut(s) 228
XspI CTAG 2 cut(s) 131, 444
ZrmI AGTACT 1 cut(s) 318
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.