Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0011984

Physical Location & Seq

Genomic Coordinates Forward (+)

24132759 .. 24140204

Gene Structure 7446 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1470 bp

ATGGATTCGCCGTCGTCGTCGACGACGCGGATCACAGCGCGGTCGTCGATGATTGACTCTTTCCGGAGCTGTACCCTATCGGGTATGACGATTGATAAGGAGGACCTCAAGAAGAAGCTCTTGATGCCGCAGTACCTCCGCTTCGCTATGCGCGATTCCATACGGTTTAAGGACCCAAGAGCCGGCGAGAGCTGGCTTCCGGGAAGGCGAGACCTCGTCGCCACCGCCGCCGTCGGGAATGTGGAGGAAAACCCGCCGGAGTCGCCCATGGTTGTGTTTATAAATCCGCGAAGCGGTGGAAGGCACGGCCCTATGCTCAAGGAGAGGCTCCAAATGTTGATGGGAGAAGAACAGGTTTTTGACCTCTCAGATGTGAAGCCTCACGAATTTGTTCAGTATGGGTTGGGTTGTCTAGAGATATTGACTGATCTTGGTGATGTTTGTGCCAAAGAGTGTCGTCAAAAGATCAGGGTTATGGTTGCAGGGGGTGATGGTACAGTCGGTTGGGTACTTGGTTGCCTCTCTGAACTCCAGAAACAGGGTCGGGAACCAGTTCCTCCAGTAGGAGTTATTCCACTTGGCACGGGAAATGATCTTTCCAGGAGTTTTGGTTGGGGTGGTTCATTCCCTTTTGCCTGGAAATCGGCCATTAAAAAAACTCTGTTCAGGGCTACTGCAGGTCCAATATGCCGTTTGGATAGTTGGCATATTGTGCTGTCAATGCCAGCCGGTACAGATATGGATCCTCCCCATTCTTTGAAGCTTACTGAAGACTGCACTCTTGATGAGACTGGATTCGAAATTCAAGGGGATTTGCCTGAGAAAGCTACTTGCTACGAAGGAGTGTTTTATAATTACTTCAGTATAGGAATGGATGCCCAAGTTGCTTATGGATTCCACCATTTACGAAACGAAAAACCCTATCTTGCACAAGGTCCAATTTCGAATAAGTTGGTTTACTCAGGTTATAGTTGCACTCAAGGTTGGTTTTTCACACCTTGCACTAGCGATCCAGGTTTAAGGGCACTAAAGAACATCCTAAGGATCCATGTCAAAAAGGTAAATTGCTCAGAGTGGGAGCAGATTCCAGTGCCTTCAAGCGTACGGGCAATTGTTGTTCTAAATCTTCATAACTATGGGAGTGGAAGAAATCCATGGGGTAATCTGAAGCCAGAGTACTTGGAAAAGAGAGGATTTGTGGAGGCCCATGCTGATGATGGCCTTCTTGAAATTTTTGGCCTGAAACAAGGATGGCATGCATCTTTTGTTATGGTTGAACTCATCTCTGCGAAACACATTGCTCAGGCTGCTGCGATTCGGTTGGAAGTTAGAGGTGGGGAATGGAGAAATGCATATATGCAGATGGATGGGGAACCATGGAAGCAGCCAATGCATGAGGAGTATTCAACACTAGTGGAAATTAAGAGGGTTCCGTTTCAATCACTGATGATTCATGGAGAAGACCACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004143 GO:0005575 GO:0005623 GO:0005886 GO:0005911 GO:0006793 GO:0006796 GO:0007275 GO:0008150 GO:0008152 GO:0009506 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0022622 GO:0030054 GO:0032501 GO:0032502 GO:0044237 GO:0044464 GO:0048364 GO:0048366 GO:0048367 GO:0048731 GO:0048827 GO:0048856 GO:0055044 GO:0071944 GO:0099402

KEGG Pathways

Metabolic & Signaling

ko00561 ko00564 ko01100 ko01110 ko04070 ko04072 ko05231 map00561 map00564 map01100 map01110 map04070 map04072 map05231

Pfam Domains

Protein Families

DAGK_acc DAGK_cat

Protein Analysis

490

Amino Acids

54.67

Weight (kDa)

6.41

Isoelectric Point (pI)

51.53

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DAGK_cat	PF00781	90 - 221	1.7e-23	Diacylglycerol kinase catalytic domain
DAGK_acc	PF00609	282 - 459	1e-42	Diacylglycerol kinase accessory domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011984)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G18730 AT4G30340 AT4G30340 AT5G57690
fragaria_vesca	FvH4_4g08400 FvH4_4g08400
malus_domestica	MD13G1262800.v1.1 MD16G1264500.v1.1
prunus_persica	Prupe.1G094900_v2.0.a1
pyrus_communis	pycom13g24340 pycom16g23310
rosa_chinensis	RchiOBHm_Chr4g0404101
rosa_laevigata	RLG00000008925
rosa_multiflora	Rmu_sc0006579.1_g000001
rosa_roxburghii	Rroxscaffold_5G00348640
rosa_rugosa	Rorug04G0046900 Rorug04G0047000
rosa_samantha	Rh4DG115900
rosa_wichuraiana	Rw4G009980

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	281, 852
Acc36I	ACCTGC	1 cut(s)	668
AccI	GTMKAC	1 cut(s)	20
AccII	CGCG	4 cut(s)	28, 40, 153, 289
AccIII	TCCGGA	1 cut(s)	63
AciI	CCGC	9 cut(s)	28, 40, 128, 139, 225, 228, 254, 287, 294
AclWI	GGATC	6 cut(s)	38, 737, 750, 1004, 1039, 1052
AcoI	YGGCCR	1 cut(s)	645
AcsI	RAATTY	3 cut(s)	386, 801, 1230
AcuI	CTGAAG	3 cut(s)	789, 844, 1187
AfaI	GTAC	7 cut(s)	73, 134, 496, 510, 733, 1104, 1178
AfiI	CCNNNNNNNGG	5 cut(s)	182, 234, 293, 538, 563
AgsI	TTSAA	7 cut(s)	760, 806, 1098, 1229, 1277, 1407, 1439
AhlI	ACTAGT	1 cut(s)	1411
AjnI	CCWGG	3 cut(s)	599, 635, 1012
AluBI	AGCT	5 cut(s)	69, 118, 192, 763, 827
AluI	AGCT	5 cut(s)	69, 118, 192, 763, 827
Alw26I	GTCTC	2 cut(s)	204, 782
AlwI	GGATC	6 cut(s)	38, 737, 750, 1004, 1039, 1052
Aor13HI	TCCGGA	1 cut(s)	63
AoxI	GGCC	5 cut(s)	307, 645, 1203, 1219, 1237
ApeKI	GCWGC	3 cut(s)	1307, 1310, 1384
ApoI	RAATTY	3 cut(s)	386, 801, 1230
Asp700I	GAANNNNTTC	2 cut(s)	390, 552
AspLEI	GCGC	2 cut(s)	40, 153
AspS9I	GGNCC	6 cut(s)	103, 172, 308, 680, 935, 1204
AsuC2I	CCSGG	1 cut(s)	201
AsuHPI	GGTGA	2 cut(s)	446, 500
AsuII	TTCGAA	2 cut(s)	798, 944
AvaII	GGWCC	4 cut(s)	103, 172, 680, 935
AxyI	CCTNAGG	1 cut(s)	1040
BaeGI	GKGCMC	1 cut(s)	1027
BamHI	GGATCC	2 cut(s)	742, 1044
BarI	GAAGNNNNNNTAC	4 cut(s)	125, 157, 1160, 1192
BbsI	GAAGAC	1 cut(s)	777
BbvI	GCAGC	3 cut(s)	1294, 1297, 1396
BccI	CCATC	6 cut(s)	334, 485, 1211, 1245, 1357, 1361
BceAI	ACGGC	3 cut(s)	215, 322, 675
BciT130I	CCWGG	3 cut(s)	601, 637, 1014
BcnI	CCSGG	1 cut(s)	201
BcoDI	GTCTC	2 cut(s)	204, 782
BcuI	ACTAGT	1 cut(s)	1411
BfaI	CTAG	3 cut(s)	413, 1005, 1412
BfmI	CTRYAG	1 cut(s)	675
BfuAI	ACCTGC	1 cut(s)	668
BisI	GCNGC	5 cut(s)	128, 228, 1308, 1311, 1385
BlsI	GCNGC	5 cut(s)	129, 229, 1309, 1312, 1386
BmcAI	AGTACT	1 cut(s)	1178
Bme1390I	CCNGG	4 cut(s)	201, 601, 637, 1014
Bme18I	GGWCC	4 cut(s)	103, 172, 680, 935
BmgT120I	GGNCC	6 cut(s)	103, 172, 308, 680, 935, 1204
BmiI	GGNNCC	7 cut(s)	174, 329, 549, 744, 1046, 1374, 1431
BmrFI	CCNGG	4 cut(s)	201, 601, 637, 1014
BmsI	GCATC	3 cut(s)	114, 865, 1268
BpiI	GAAGAC	1 cut(s)	777
BpmI	CTGGAG	2 cut(s)	515, 543
Bpu10I	CCTNAGC	1 cut(s)	1302
Bpu14I	TTCGAA	2 cut(s)	798, 944
BpuEI	CTTGAG	3 cut(s)	92, 302, 963
BpuMI	CCSGG	1 cut(s)	201
BsaBI	GATNNNNATC	1 cut(s)	741
BsaI	GGTCTC	1 cut(s)	204
BsaJI	CCNNGG	3 cut(s)	267, 1154, 1376
BsaWI	WCCGGW	1 cut(s)	63
BsaXI	ACNNNNNCTCC	2 cut(s)	595, 625
Bsc4I	CCNNNNNNNGG	5 cut(s)	182, 234, 293, 538, 563
Bse118I	RCCGGY	2 cut(s)	182, 728
Bse1I	ACTGG	4 cut(s)	551, 560, 796, 1088
Bse21I	CCTNAGG	1 cut(s)	1040
Bse3DI	GCAATG	1 cut(s)	1296
Bse8I	GATNNNNATC	1 cut(s)	741
BseAI	TCCGGA	1 cut(s)	63
BseBI	CCWGG	3 cut(s)	601, 637, 1014
BseDI	CCNNGG	3 cut(s)	267, 1154, 1376
BseGI	GGATG	4 cut(s)	880, 1035, 1256, 1372
BseJI	GATNNNNATC	1 cut(s)	741
BseLI	CCNNNNNNNGG	5 cut(s)	182, 234, 293, 538, 563
BseMI	GCAATG	1 cut(s)	1296
BseMII	CTCAG	5 cut(s)	381, 810, 975, 1083, 1316
BseNI	ACTGG	4 cut(s)	551, 560, 796, 1088
BseRI	GAGGAG	1 cut(s)	1412
BseSI	GKGCMC	1 cut(s)	1027
BseXI	GCAGC	3 cut(s)	1294, 1297, 1396
BsgI	GTGCAG	1 cut(s)	760
Bsh1236I	CGCG	4 cut(s)	28, 40, 153, 289
Bsh1285I	CGRYCG	1 cut(s)	44
BshFI	GGCC	5 cut(s)	309, 647, 1205, 1221, 1239
BsiEI	CGRYCG	1 cut(s)	44
BsiSI	CCGG	5 cut(s)	64, 183, 200, 257, 729
BsiWI	CGTACG	1 cut(s)	1102
BslI	CCNNNNNNNGG	5 cut(s)	182, 234, 293, 538, 563
BsmAI	GTCTC	2 cut(s)	204, 782
BsnI	GGCC	5 cut(s)	309, 647, 1205, 1221, 1239
Bso31I	GGTCTC	1 cut(s)	204
Bsp119I	TTCGAA	2 cut(s)	798, 944
Bsp1286I	GDGCHC	1 cut(s)	1027
Bsp13I	TCCGGA	1 cut(s)	63
Bsp143I	GATC	7 cut(s)	30, 427, 465, 592, 742, 1009, 1044
Bsp19I	CCATGG	3 cut(s)	267, 1154, 1376
BspACI	CCGC	9 cut(s)	28, 40, 128, 139, 225, 228, 254, 287, 294
BspANI	GGCC	5 cut(s)	309, 647, 1205, 1221, 1239
BspCNI	CTCAG	5 cut(s)	380, 811, 974, 1082, 1315
BspEI	TCCGGA	1 cut(s)	63
BspFNI	CGCG	4 cut(s)	28, 40, 153, 289
BspLI	GGNNCC	7 cut(s)	174, 329, 549, 744, 1046, 1374, 1431
BspMAI	CTGCAG	1 cut(s)	679
BspMI	ACCTGC	1 cut(s)	668
BspPI	GGATC	6 cut(s)	38, 737, 750, 1004, 1039, 1052
BspT104I	TTCGAA	2 cut(s)	798, 944
BspTNI	GGTCTC	1 cut(s)	204
BsrDI	GCAATG	1 cut(s)	1296
BsrFI	RCCGGY	2 cut(s)	182, 728
BsrI	ACTGG	4 cut(s)	551, 560, 796, 1088
BssAI	RCCGGY	2 cut(s)	182, 728
BssECI	CCNNGG	3 cut(s)	267, 1154, 1376
BssMI	GATC	7 cut(s)	30, 427, 465, 592, 742, 1009, 1044
BssT1I	CCWWGG	3 cut(s)	267, 1154, 1376
Bst2UI	CCWGG	3 cut(s)	601, 637, 1014
Bst4CI	ACNGT	2 cut(s)	165, 499
BstAPI	GCANNNNNTGC	2 cut(s)	712, 1390
BstBI	TTCGAA	2 cut(s)	798, 944
BstC8I	GCNNGC	4 cut(s)	184, 194, 726, 1257
BstDEI	CTNAG	6 cut(s)	367, 819, 961, 1040, 1069, 1302
BstDSI	CCRYGG	3 cut(s)	267, 1154, 1376
BstENI	CCTNNNNNAGG	1 cut(s)	561
BstF5I	GGATG	4 cut(s)	880, 1035, 1256, 1372
BstFNI	CGCG	4 cut(s)	28, 40, 153, 289
BstHHI	GCGC	2 cut(s)	40, 153
BstKTI	GATC	7 cut(s)	33, 430, 468, 595, 745, 1012, 1047
BstMAI	GTCTC	2 cut(s)	204, 782
BstMBI	GATC	7 cut(s)	30, 427, 465, 592, 742, 1009, 1044
BstMCI	CGRYCG	1 cut(s)	44
BstMWI	GCNNNNNNNGC	8 cut(s)	124, 227, 262, 712, 721, 884, 1307, 1390
BstNI	CCWGG	3 cut(s)	601, 637, 1014
BstNSI	RCATGY	1 cut(s)	1259
BstSCI	CCNGG	4 cut(s)	199, 599, 635, 1012
BstSFI	CTRYAG	1 cut(s)	675
BstSLI	GKGCMC	1 cut(s)	1027
BstUI	CGCG	4 cut(s)	28, 40, 153, 289
BstV1I	GCAGC	3 cut(s)	1294, 1297, 1396
BstV2I	GAAGAC	1 cut(s)	777
BstX2I	RGATCY	2 cut(s)	742, 1044
BstYI	RGATCY	2 cut(s)	742, 1044
Bsu36I	CCTNAGG	1 cut(s)	1040
BsuRI	GGCC	5 cut(s)	309, 647, 1205, 1221, 1239
BtgI	CCRYGG	3 cut(s)	267, 1154, 1376
BtsCI	GGATG	4 cut(s)	880, 1035, 1256, 1372
BtsIMutI	CAGTG	3 cut(s)	1095, 1442, 1465
BveI	ACCTGC	1 cut(s)	668
Cac8I	GCNNGC	4 cut(s)	184, 194, 726, 1257
CfoI	GCGC	2 cut(s)	40, 153
Cfr10I	RCCGGY	2 cut(s)	182, 728
Cfr13I	GGNCC	6 cut(s)	103, 172, 308, 680, 935, 1204
CseI	GACGC	1 cut(s)	34
Csp6I	GTAC	7 cut(s)	72, 133, 495, 509, 732, 1103, 1177
CspCI	CAANNNNNGTGG	2 cut(s)	1395, 1430
CviAII	CATG	8 cut(s)	268, 1049, 1155, 1208, 1256, 1377, 1394, 1454
CviQI	GTAC	7 cut(s)	72, 133, 495, 509, 732, 1103, 1177
DdeI	CTNAG	6 cut(s)	367, 819, 961, 1040, 1069, 1302
DpnI	GATC	7 cut(s)	32, 429, 467, 594, 744, 1011, 1046
DpnII	GATC	7 cut(s)	30, 427, 465, 592, 742, 1009, 1044
EaeI	YGGCCR	1 cut(s)	645
Eco130I	CCWWGG	3 cut(s)	267, 1154, 1376
Eco31I	GGTCTC	1 cut(s)	204
Eco47I	GGWCC	4 cut(s)	103, 172, 680, 935
Eco57I	CTGAAG	3 cut(s)	789, 844, 1187
Eco81I	CCTNAGG	1 cut(s)	1040
EcoNI	CCTNNNNNAGG	1 cut(s)	561
EcoO109I	RGGNCCY	2 cut(s)	103, 172
EcoRII	CCWGG	3 cut(s)	599, 635, 1012
EcoT14I	CCWWGG	3 cut(s)	267, 1154, 1376
EcoT22I	ATGCAT	3 cut(s)	1261, 1354, 1395
ErhI	CCWWGG	3 cut(s)	267, 1154, 1376
FaeI	CATG	8 cut(s)	271, 1052, 1158, 1211, 1259, 1380, 1397, 1457
FalI	AAGNNNNNCTT	2 cut(s)	104, 136
FatI	CATG	8 cut(s)	267, 1048, 1154, 1207, 1255, 1376, 1393, 1453
FauI	CCCGC	1 cut(s)	261
FblI	GTMKAC	1 cut(s)	20
Fnu4HI	GCNGC	5 cut(s)	128, 228, 1308, 1311, 1385
FokI	GGATG	4 cut(s)	887, 1022, 1263, 1379
Fsp4HI	GCNGC	5 cut(s)	128, 228, 1308, 1311, 1385
FspBI	CTAG	3 cut(s)	413, 1005, 1412
GlaI	GCGC	2 cut(s)	39, 152
GluI	GCNGC	5 cut(s)	128, 228, 1308, 1311, 1385
GsuI	CTGGAG	2 cut(s)	515, 543
HaeIII	GGCC	5 cut(s)	309, 647, 1205, 1221, 1239
HapII	CCGG	5 cut(s)	64, 183, 200, 257, 729
HgaI	GACGC	1 cut(s)	34
HhaI	GCGC	2 cut(s)	40, 153
Hin1II	CATG	8 cut(s)	271, 1052, 1158, 1211, 1259, 1380, 1397, 1457
Hin6I	GCGC	2 cut(s)	38, 151
HinP1I	GCGC	2 cut(s)	38, 151
HincII	GTYRAC	1 cut(s)	21
HindII	GTYRAC	1 cut(s)	21
HindIII	AAGCTT	1 cut(s)	761
HinfI	GANTC	9 cut(s)	5, 56, 155, 260, 795, 894, 1084, 1315, 1450
HpaII	CCGG	5 cut(s)	64, 183, 200, 257, 729
HphI	GGTGA	2 cut(s)	446, 500
Hpy166II	GTNNAC	2 cut(s)	21, 958
Hpy188I	TCNGA	4 cut(s)	370, 526, 1072, 1167
Hpy8I	GTNNAC	2 cut(s)	21, 958
Hpy99I	CGWCG	8 cut(s)	16, 19, 22, 25, 28, 49, 221, 236
HpyAV	CCTTC	5 cut(s)	198, 294, 833, 1104, 1232
HpyCH4III	ACNGT	2 cut(s)	165, 499
HpyF10VI	GCNNNNNNNGC	8 cut(s)	124, 227, 262, 712, 721, 884, 1307, 1390
HpyF3I	CTNAG	6 cut(s)	367, 819, 961, 1040, 1069, 1302
Hsp92II	CATG	8 cut(s)	271, 1052, 1158, 1211, 1259, 1380, 1397, 1457
HspAI	GCGC	2 cut(s)	38, 151
Kpn2I	TCCGGA	1 cut(s)	63
KroI	GCCGGC	1 cut(s)	182
KroNI	GCCGGC	1 cut(s)	184
Kzo9I	GATC	7 cut(s)	30, 427, 465, 592, 742, 1009, 1044
LmnI	GCTCC	3 cut(s)	66, 333, 1078
Lsp1109I	GCAGC	3 cut(s)	1294, 1297, 1396
LweI	GCATC	3 cut(s)	114, 865, 1268
MaeI	CTAG	3 cut(s)	413, 1005, 1412
MalI	GATC	7 cut(s)	32, 429, 467, 594, 744, 1011, 1046
MboI	GATC	7 cut(s)	30, 427, 465, 592, 742, 1009, 1044
MboII	GAAGA	5 cut(s)	124, 359, 782, 1118, 1158
MfeI	CAATTG	1 cut(s)	1110
MflI	RGATCY	2 cut(s)	742, 1044
MhlI	GDGCHC	1 cut(s)	1027
MluCI	AATT	8 cut(s)	386, 801, 853, 939, 1063, 1110, 1230, 1419
MlyI	GAGTC	2 cut(s)	50, 269
MmeI	TCCRAC	1 cut(s)	1302
Mph1103I	ATGCAT	3 cut(s)	1261, 1354, 1395
MroI	TCCGGA	1 cut(s)	63
MroNI	GCCGGC	1 cut(s)	182
MroXI	GAANNNNTTC	2 cut(s)	390, 552
MseI	TTAA	4 cut(s)	168, 651, 1019, 1422
MslI	CAYNNNNRTG	3 cut(s)	272, 1134, 1212
MspI	CCGG	5 cut(s)	64, 183, 200, 257, 729
MspR9I	CCNGG	4 cut(s)	201, 601, 637, 1014
MunI	CAATTG	1 cut(s)	1110
MvaI	CCWGG	3 cut(s)	601, 637, 1014
MvnI	CGCG	4 cut(s)	28, 40, 153, 289
MwoI	GCNNNNNNNGC	8 cut(s)	124, 227, 262, 712, 721, 884, 1307, 1390
NaeI	GCCGGC	1 cut(s)	184
NciI	CCSGG	1 cut(s)	201
NcoI	CCATGG	3 cut(s)	267, 1154, 1376
NdeII	GATC	7 cut(s)	30, 427, 465, 592, 742, 1009, 1044
NgoMIV	GCCGGC	1 cut(s)	182
NlaIII	CATG	8 cut(s)	271, 1052, 1158, 1211, 1259, 1380, 1397, 1457
NlaIV	GGNNCC	7 cut(s)	174, 329, 549, 744, 1046, 1374, 1431
NsiI	ATGCAT	3 cut(s)	1261, 1354, 1395
NspI	RCATGY	1 cut(s)	1259
NspV	TTCGAA	2 cut(s)	798, 944
PaeI	GCATGC	1 cut(s)	1259
PcsI	WCGNNNNNNNCGW	4 cut(s)	14, 20, 86, 150
PdiI	GCCGGC	1 cut(s)	184
PdmI	GAANNNNTTC	2 cut(s)	390, 552
PfeI	GAWTC	7 cut(s)	5, 155, 795, 894, 1084, 1315, 1450
Pfl23II	CGTACG	1 cut(s)	1102
PflFI	GACNNNGTC	1 cut(s)	215
PfoI	TCCNGGA	2 cut(s)	199, 599
PkrI	GCNGC	5 cut(s)	129, 229, 1309, 1312, 1386
PleI	GAGTC	2 cut(s)	50, 268
PpsI	GAGTC	2 cut(s)	50, 268
PpuMI	RGGWCCY	2 cut(s)	103, 172
PsiI	TTATAA	2 cut(s)	281, 852
Psp5II	RGGWCCY	2 cut(s)	103, 172
Psp6I	CCWGG	3 cut(s)	599, 635, 1012
PspGI	CCWGG	3 cut(s)	599, 635, 1012
PspLI	CGTACG	1 cut(s)	1102
PspN4I	GGNNCC	7 cut(s)	174, 329, 549, 744, 1046, 1374, 1431
PspPI	GGNCC	6 cut(s)	103, 172, 308, 680, 935, 1204
PspPPI	RGGWCCY	2 cut(s)	103, 172
PstI	CTGCAG	1 cut(s)	679
PsuI	RGATCY	2 cut(s)	742, 1044
PsyI	GACNNNGTC	1 cut(s)	215
RsaI	GTAC	7 cut(s)	73, 134, 496, 510, 733, 1104, 1178
RsaNI	GTAC	7 cut(s)	72, 133, 495, 509, 732, 1103, 1177
RseI	CAYNNNNRTG	3 cut(s)	272, 1134, 1212
SalI	GTCGAC	1 cut(s)	19
SaqAI	TTAA	4 cut(s)	168, 651, 1019, 1422
SatI	GCNGC	5 cut(s)	128, 228, 1308, 1311, 1385
Sau3AI	GATC	7 cut(s)	30, 427, 465, 592, 742, 1009, 1044
Sau96I	GGNCC	6 cut(s)	103, 172, 308, 680, 935, 1204
ScaI	AGTACT	1 cut(s)	1178
SchI	GAGTC	2 cut(s)	50, 269
ScrFI	CCNGG	4 cut(s)	201, 601, 637, 1014
SduI	GDGCHC	1 cut(s)	1027
SfaNI	GCATC	3 cut(s)	114, 865, 1268
SfcI	CTRYAG	1 cut(s)	675
SfuI	TTCGAA	2 cut(s)	798, 944
SgrDI	CGTCGACG	1 cut(s)	19
SinI	GGWCC	4 cut(s)	103, 172, 680, 935
SmiMI	CAYNNNNRTG	3 cut(s)	272, 1134, 1212
SmlI	CTYRAG	3 cut(s)	107, 317, 978
SmoI	CTYRAG	3 cut(s)	107, 317, 978
SpeI	ACTAGT	1 cut(s)	1411
SphI	GCATGC	1 cut(s)	1259
Sse9I	AATT	8 cut(s)	386, 801, 853, 939, 1063, 1110, 1230, 1419
SsiI	CCGC	9 cut(s)	28, 40, 128, 139, 225, 228, 254, 287, 294
SspMI	CTAG	3 cut(s)	413, 1005, 1412
StyD4I	CCNGG	4 cut(s)	199, 599, 635, 1012
StyI	CCWWGG	3 cut(s)	267, 1154, 1376
TaaI	ACNGT	2 cut(s)	165, 499
TaqI	TCGA	4 cut(s)	20, 47, 798, 944
TasI	AATT	8 cut(s)	386, 801, 853, 939, 1063, 1110, 1230, 1419
TatI	WGTACW	1 cut(s)	1176
TauI	GCSGC	2 cut(s)	130, 230
TfiI	GAWTC	7 cut(s)	5, 155, 795, 894, 1084, 1315, 1450
Tru1I	TTAA	4 cut(s)	168, 651, 1019, 1422
Tru9I	TTAA	4 cut(s)	168, 651, 1019, 1422
TscAI	CASTG	2 cut(s)	1095, 1449
TseI	GCWGC	3 cut(s)	1307, 1310, 1384
TspDTI	ATGAA	3 cut(s)	612, 1118, 1442
TspGWI	ACGGA	1 cut(s)	1422
TspRI	CASTG	2 cut(s)	1095, 1449
Tth111I	GACNNNGTC	1 cut(s)	215
VpaK11BI	GGWCC	4 cut(s)	103, 172, 680, 935
XagI	CCTNNNNNAGG	1 cut(s)	561
XapI	RAATTY	3 cut(s)	386, 801, 1230
XbaI	TCTAGA	1 cut(s)	412
XceI	RCATGY	1 cut(s)	1259
XcmI	CCANNNNNNNNNTGG	2 cut(s)	887, 1214
XmiI	GTMKAC	1 cut(s)	20
XmnI	GAANNNNTTC	2 cut(s)	390, 552
XspI	CTAG	3 cut(s)	413, 1005, 1412
ZrmI	AGTACT	1 cut(s)	1178
Zsp2I	ATGCAT	3 cut(s)	1261, 1354, 1395

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.