pycom16g26270

single fertilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0013480
Physical Location & Seq
Forward (+)
28645240 .. 28645722
483 bp
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UTR
Exon/CDS
Intron
pycom16g26270.1

Sequence Viewer

Length: 483 bp
ATGACGGCCGGCGTGGTTGTGTACCCTGATGCCTGTGAATCTGAGGTCTCCAAAGGTGAGGTTGGGAATGTCGTTGCCAATGTTGTGAATGGGAATGTTGGGATTGGGAATGGGAATCAGAGCGATAATGGGGTTTCTAAGGACGACACCGACACCAACGCTGACGGCTCTTGTGTCTCTTGTGTCTTTGTAACCAGGAGTGATGCCATCGTTTCCAAAGATCCTGTCCATTCTCATCTCAATAGCAGCGTTGTTCAGAACAATGGTGCTGTTGGTCTTCACTTGGAGCCTCAAAAGGACAATTCCCAATCTCATTTGCTCAACAAGGCTGCCGCAGAAGCCCCACATCTCAATGACGGAGAGGTGGTGGTGTCTGAGCGAGCAGAGACTGACCAGGATCCAAAAGAAGCCGAGGAGGTGCCCATGGAAGATGCTTCGAGGGATCTAAGCCAACAGACAAAGCATTATTCGATGCCAACCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

161

Amino Acids

16.81

Weight (kDa)

4.44

Isoelectric Point (pI)

35.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013480)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20970
fragaria_vesca FvH4_5g26490 FvH4_5g26490
malus_domestica MD06G1001600.v1.1
prunus_persica Prupe.5G002400_v2.0.a1 Prupe.5G002400_v2.0.a1
pyrus_communis pycom06g00170 pycom16g26270 pycom16g26280
rosa_chinensis RchiOBHm_Chr7g0217401
rosa_laevigata RLG00000002480
rosa_roxburghii Rroxscaffold_3G00242210
rosa_rugosa Rorug07G0168900
rosa_samantha Rh7AG308500 Rh7BG299200 Rh7CG327800 Rh7DG307800
rosa_wichuraiana Rw7G026160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 418
AciI CCGC 1 cut(s) 333
AclWI GGATC 4 cut(s) 215, 392, 405, 450
AcoI YGGCCR 1 cut(s) 6
AfaI GTAC 1 cut(s) 23
AjnI CCWGG 2 cut(s) 194, 393
Alw26I GTCTC 3 cut(s) 52, 181, 380
AlwI GGATC 4 cut(s) 215, 392, 405, 450
AlwNI CAGNNNCTG 1 cut(s) 389
AoxI GGCC 1 cut(s) 6
ApeKI GCWGC 2 cut(s) 246, 329
ArsI GACNNNNNNTTYG 2 cut(s) 210, 242
AsuHPI GGTGA 1 cut(s) 68
BaeGI GKGCMC 1 cut(s) 423
BamHI GGATCC 1 cut(s) 397
BanI GGYRCC 1 cut(s) 418
BbsI GAAGAC 1 cut(s) 269
BbvI GCAGC 2 cut(s) 258, 316
BccI CCATC 1 cut(s) 215
BceAI ACGGC 2 cut(s) 21, 181
BciT130I CCWGG 2 cut(s) 196, 395
BcoDI GTCTC 3 cut(s) 52, 181, 380
BisI GCNGC 3 cut(s) 247, 330, 333
BlsI GCNGC 3 cut(s) 248, 331, 334
Bme1390I CCNGG 2 cut(s) 196, 395
BmiI GGNNCC 3 cut(s) 288, 399, 420
BmrFI CCNGG 2 cut(s) 196, 395
BmsI GCATC 4 cut(s) 19, 193, 421, 462
BpiI GAAGAC 1 cut(s) 269
BsaI GGTCTC 1 cut(s) 52
BsaJI CCNNGG 2 cut(s) 411, 423
BsaXI ACNNNNNCTCC 2 cut(s) 351, 381
Bse118I RCCGGY 1 cut(s) 8
BseBI CCWGG 2 cut(s) 196, 395
BseDI CCNNGG 2 cut(s) 411, 423
BseMII CTCAG 2 cut(s) 33, 366
BseRI GAGGAG 1 cut(s) 428
BseSI GKGCMC 1 cut(s) 423
BseX3I CGGCCG 1 cut(s) 6
BseXI GCAGC 2 cut(s) 258, 316
Bsh1285I CGRYCG 1 cut(s) 9
BshFI GGCC 1 cut(s) 8
BshNI GGYRCC 1 cut(s) 418
BsiEI CGRYCG 1 cut(s) 9
BsiSI CCGG 1 cut(s) 9
BsmAI GTCTC 3 cut(s) 52, 181, 380
BsnI GGCC 1 cut(s) 8
Bso31I GGTCTC 1 cut(s) 52
Bsp1286I GDGCHC 1 cut(s) 423
Bsp143I GATC 3 cut(s) 220, 397, 442
Bsp19I CCATGG 1 cut(s) 423
BspACI CCGC 1 cut(s) 333
BspANI GGCC 1 cut(s) 8
BspCNI CTCAG 2 cut(s) 34, 367
BspLI GGNNCC 3 cut(s) 288, 399, 420
BspPI GGATC 4 cut(s) 215, 392, 405, 450
BspT107I GGYRCC 1 cut(s) 418
BspTNI GGTCTC 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 8
BssAI RCCGGY 1 cut(s) 8
BssECI CCNNGG 2 cut(s) 411, 423
BssMI GATC 3 cut(s) 220, 397, 442
BssT1I CCWWGG 1 cut(s) 423
Bst2UI CCWGG 2 cut(s) 196, 395
BstC8I GCNNGC 2 cut(s) 10, 381
BstDEI CTNAG 4 cut(s) 42, 138, 375, 446
BstDSI CCRYGG 1 cut(s) 423
BstKTI GATC 3 cut(s) 223, 400, 445
BstMAI GTCTC 3 cut(s) 52, 181, 380
BstMBI GATC 3 cut(s) 220, 397, 442
BstMCI CGRYCG 1 cut(s) 9
BstMWI GCNNNNNNNGC 1 cut(s) 338
BstNI CCWGG 2 cut(s) 196, 395
BstSCI CCNGG 2 cut(s) 194, 393
BstSLI GKGCMC 1 cut(s) 423
BstV1I GCAGC 2 cut(s) 258, 316
BstV2I GAAGAC 1 cut(s) 269
BstX2I RGATCY 3 cut(s) 220, 397, 442
BstYI RGATCY 3 cut(s) 220, 397, 442
BstZI CGGCCG 1 cut(s) 6
BsuRI GGCC 1 cut(s) 8
BtgI CCRYGG 1 cut(s) 423
Cac8I GCNNGC 2 cut(s) 10, 381
CaiI CAGNNNCTG 1 cut(s) 389
Cfr10I RCCGGY 1 cut(s) 8
Csp6I GTAC 1 cut(s) 22
CviAII CATG 1 cut(s) 424
CviJI RGCY 7 cut(s) 8, 168, 289, 329, 341, 410, 450
CviKI_1 RGCY 7 cut(s) 8, 168, 289, 329, 341, 410, 450
CviQI GTAC 1 cut(s) 22
DdeI CTNAG 4 cut(s) 42, 138, 375, 446
DpnI GATC 3 cut(s) 222, 399, 444
DpnII GATC 3 cut(s) 220, 397, 442
EaeI YGGCCR 1 cut(s) 6
EagI CGGCCG 1 cut(s) 6
EclXI CGGCCG 1 cut(s) 6
Eco130I CCWWGG 1 cut(s) 423
Eco31I GGTCTC 1 cut(s) 52
Eco52I CGGCCG 1 cut(s) 6
EcoRII CCWGG 2 cut(s) 194, 393
EcoT14I CCWWGG 1 cut(s) 423
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 1 cut(s) 427
FaiI YATR 1 cut(s) 425
FatI CATG 1 cut(s) 423
Fnu4HI GCNGC 3 cut(s) 247, 330, 333
Fsp4HI GCNGC 3 cut(s) 247, 330, 333
GluI GCNGC 3 cut(s) 247, 330, 333
HaeIII GGCC 1 cut(s) 8
HapII CCGG 1 cut(s) 9
Hin1II CATG 1 cut(s) 427
HinfI GANTC 2 cut(s) 38, 115
HpaII CCGG 1 cut(s) 9
HphI GGTGA 1 cut(s) 68
Hpy166II GTNNAC 1 cut(s) 22
Hpy188I TCNGA 4 cut(s) 43, 120, 258, 376
Hpy8I GTNNAC 1 cut(s) 22
HpyF10VI GCNNNNNNNGC 1 cut(s) 338
HpyF3I CTNAG 4 cut(s) 42, 138, 375, 446
Hsp92II CATG 1 cut(s) 427
KroI GCCGGC 1 cut(s) 8
KroNI GCCGGC 1 cut(s) 10
Kzo9I GATC 3 cut(s) 220, 397, 442
LmnI GCTCC 1 cut(s) 286
LpnPI CCDG 8 cut(s) 22, 39, 46, 181, 208, 237, 380, 407
Lsp1109I GCAGC 2 cut(s) 258, 316
LweI GCATC 4 cut(s) 19, 193, 421, 462
MaeIII GTNAC 1 cut(s) 190
MalI GATC 3 cut(s) 222, 399, 444
MboI GATC 3 cut(s) 220, 397, 442
MboII GAAGA 2 cut(s) 269, 440
MflI RGATCY 3 cut(s) 220, 397, 442
MhlI GDGCHC 1 cut(s) 423
MluCI AATT 1 cut(s) 301
MnlI CCTC 7 cut(s) 37, 52, 300, 355, 406, 409, 432
MroNI GCCGGC 1 cut(s) 8
MslI CAYNNNNRTG 1 cut(s) 351
MspI CCGG 1 cut(s) 9
MspR9I CCNGG 2 cut(s) 196, 395
MvaI CCWGG 2 cut(s) 196, 395
MwoI GCNNNNNNNGC 1 cut(s) 338
NaeI GCCGGC 1 cut(s) 10
NcoI CCATGG 1 cut(s) 423
NdeII GATC 3 cut(s) 220, 397, 442
NgoMIV GCCGGC 1 cut(s) 8
NlaIII CATG 1 cut(s) 427
NlaIV GGNNCC 3 cut(s) 288, 399, 420
NmeAIII GCCGAG 1 cut(s) 436
PdiI GCCGGC 1 cut(s) 10
PfeI GAWTC 2 cut(s) 38, 115
PkrI GCNGC 3 cut(s) 248, 331, 334
Psp6I CCWGG 2 cut(s) 194, 393
PspGI CCWGG 2 cut(s) 194, 393
PspN4I GGNNCC 3 cut(s) 288, 399, 420
PstNI CAGNNNCTG 1 cut(s) 389
PsuI RGATCY 3 cut(s) 220, 397, 442
RsaI GTAC 1 cut(s) 23
RsaNI GTAC 1 cut(s) 22
RseI CAYNNNNRTG 1 cut(s) 351
SatI GCNGC 3 cut(s) 247, 330, 333
Sau3AI GATC 3 cut(s) 220, 397, 442
ScrFI CCNGG 2 cut(s) 196, 395
SduI GDGCHC 1 cut(s) 423
SetI ASST 6 cut(s) 48, 58, 63, 366, 420, 482
SfaNI GCATC 4 cut(s) 19, 193, 421, 462
SmiMI CAYNNNNRTG 1 cut(s) 351
Sse9I AATT 1 cut(s) 301
SsiI CCGC 1 cut(s) 333
StyD4I CCNGG 2 cut(s) 194, 393
StyI CCWWGG 1 cut(s) 423
TaqI TCGA 2 cut(s) 437, 470
TasI AATT 1 cut(s) 301
TauI GCSGC 1 cut(s) 335
TfiI GAWTC 2 cut(s) 38, 115
TseI GCWGC 2 cut(s) 246, 329
TspGWI ACGGA 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.