single fertilization
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT1G20970 |
| fragaria_vesca | FvH4_5g26490 FvH4_5g26490 |
| malus_domestica | MD06G1001600.v1.1 |
| prunus_persica | Prupe.5G002400_v2.0.a1 Prupe.5G002400_v2.0.a1 |
| pyrus_communis | pycom06g00170 pycom16g26270 pycom16g26280 |
| rosa_chinensis | RchiOBHm_Chr7g0217401 |
| rosa_laevigata | RLG00000002480 |
| rosa_roxburghii | Rroxscaffold_3G00242210 |
| rosa_rugosa | Rorug07G0168900 |
| rosa_samantha | Rh7AG308500 Rh7BG299200 Rh7CG327800 Rh7DG307800 |
| rosa_wichuraiana | Rw7G026160 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1243 |
| AatII | GACGTC | 1 cut(s) | 915 |
| AccB7I | CCANNNNNTGG | 9 cut(s) | 739, 826, 874, 922, 1009, 1096, 1183, 1270, 1357 |
| AccII | CGCG | 1 cut(s) | 27 |
| AciI | CCGC | 7 cut(s) | 8, 150, 165, 168, 225, 231, 237 |
| AclWI | GGATC | 7 cut(s) | 341, 482, 583, 588, 1528, 3189, 3207 |
| AcoI | YGGCCR | 9 cut(s) | 589, 730, 817, 865, 1000, 1087, 1174, 1261, 1348 |
| AcsI | RAATTY | 3 cut(s) | 1963, 1996, 2867 |
| AcuI | CTGAAG | 8 cut(s) | 353, 1449, 1530, 1695, 2520, 2907, 3104, 3206 |
| AcyI | GRCGYC | 2 cut(s) | 162, 912 |
| AfaI | GTAC | 4 cut(s) | 559, 1719, 2505, 3339 |
| AflII | CTTAAG | 1 cut(s) | 3115 |
| AgeI | ACCGGT | 1 cut(s) | 2317 |
| AjnI | CCWGG | 1 cut(s) | 2166 |
| Alw21I | GWGCWC | 2 cut(s) | 1311, 2859 |
| Alw26I | GTCTC | 3 cut(s) | 209, 1838, 3026 |
| AlwI | GGATC | 7 cut(s) | 341, 482, 583, 588, 1528, 3189, 3207 |
| AlwNI | CAGNNNCTG | 6 cut(s) | 356, 1847, 2351, 2459, 2498, 2645 |
| Ama87I | CYCGRG | 1 cut(s) | 2226 |
| ApoI | RAATTY | 3 cut(s) | 1963, 1996, 2867 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 3025, 3057 |
| AseI | ATTAAT | 6 cut(s) | 795, 978, 1065, 1326, 2412, 2559 |
| AsiGI | ACCGGT | 1 cut(s) | 2317 |
| AspLEI | GCGC | 1 cut(s) | 3229 |
| AspS9I | GGNCC | 3 cut(s) | 409, 1595, 2841 |
| AsuC2I | CCSGG | 1 cut(s) | 2807 |
| AsuHPI | GGTGA | 7 cut(s) | 139, 170, 356, 497, 851, 2428, 2575 |
| AsuII | TTCGAA | 1 cut(s) | 1557 |
| AvaI | CYCGRG | 1 cut(s) | 2226 |
| AvaII | GGWCC | 1 cut(s) | 409 |
| AxyI | CCTNAGG | 1 cut(s) | 1800 |
| BaeGI | GKGCMC | 1 cut(s) | 1939 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1755, 1788 |
| BalI | TGGCCA | 6 cut(s) | 732, 1002, 1089, 1176, 1263, 1350 |
| BanII | GRGCYC | 6 cut(s) | 659, 780, 1050, 1224, 1479, 2193 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 3321, 3353 |
| Bbv12I | GWGCWC | 2 cut(s) | 1311, 2859 |
| BbvCI | CCTCAGC | 3 cut(s) | 324, 550, 786 |
| BccI | CCATC | 4 cut(s) | 122, 1148, 2480, 2627 |
| BceAI | ACGGC | 7 cut(s) | 21, 139, 832, 880, 2318, 2452, 3078 |
| BcgI | CGANNNNNNTGC | 6 cut(s) | 1034, 1068, 1208, 1242, 3047, 3081 |
| BciT130I | CCWGG | 1 cut(s) | 2168 |
| BclI | TGATCA | 1 cut(s) | 3097 |
| BcnI | CCSGG | 1 cut(s) | 2807 |
| BcoDI | GTCTC | 3 cut(s) | 209, 1838, 3026 |
| BfaI | CTAG | 4 cut(s) | 2060, 2082, 2325, 2733 |
| BfmI | CTRYAG | 4 cut(s) | 414, 1968, 3309, 3344 |
| BfrI | CTTAAG | 1 cut(s) | 3115 |
| BglII | AGATCT | 1 cut(s) | 2220 |
| Bme1390I | CCNGG | 2 cut(s) | 2168, 2807 |
| Bme18I | GGWCC | 1 cut(s) | 409 |
| BmeT110I | CYCGRG | 1 cut(s) | 2226 |
| BmgT120I | GGNCC | 3 cut(s) | 409, 1595, 2841 |
| BmiI | GGNNCC | 3 cut(s) | 133, 411, 1134 |
| BmrFI | CCNGG | 2 cut(s) | 2168, 2807 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 2012, 2044 |
| Bpu10I | CCTNAGC | 5 cut(s) | 324, 550, 786, 2452, 2599 |
| Bpu14I | TTCGAA | 1 cut(s) | 1557 |
| BpuEI | CTTGAG | 4 cut(s) | 2035, 2267, 2897, 3225 |
| BpuMI | CCSGG | 1 cut(s) | 2807 |
| BsaAI | YACGTR | 1 cut(s) | 3337 |
| BsaBI | GATNNNNATC | 2 cut(s) | 21, 1488 |
| BsaHI | GRCGYC | 2 cut(s) | 162, 912 |
| BsaI | GGTCTC | 2 cut(s) | 209, 3026 |
| BsaJI | CCNNGG | 6 cut(s) | 51, 114, 402, 597, 669, 3021 |
| BsaWI | WCCGGW | 2 cut(s) | 341, 2317 |
| Bse118I | RCCGGY | 3 cut(s) | 2317, 2545, 2590 |
| Bse1I | ACTGG | 1 cut(s) | 1583 |
| Bse21I | CCTNAGG | 1 cut(s) | 1800 |
| Bse3DI | GCAATG | 3 cut(s) | 629, 1137, 1224 |
| Bse8I | GATNNNNATC | 2 cut(s) | 21, 1488 |
| BseBI | CCWGG | 1 cut(s) | 2168 |
| BseDI | CCNNGG | 6 cut(s) | 51, 114, 402, 597, 669, 3021 |
| BseGI | GGATG | 5 cut(s) | 446, 1192, 2815, 2974, 3133 |
| BseJI | GATNNNNATC | 2 cut(s) | 21, 1488 |
| BseMI | GCAATG | 3 cut(s) | 629, 1137, 1224 |
| BseNI | ACTGG | 1 cut(s) | 1583 |
| BseRI | GAGGAG | 5 cut(s) | 266, 341, 398, 419, 803 |
| BseSI | GKGCMC | 1 cut(s) | 1939 |
| BsgI | GTGCAG | 1 cut(s) | 342 |
| Bsh1236I | CGCG | 1 cut(s) | 27 |
| BshTI | ACCGGT | 1 cut(s) | 2317 |
| BsiHKAI | GWGCWC | 2 cut(s) | 1311, 2859 |
| BsiHKCI | CYCGRG | 1 cut(s) | 2226 |
| BsiSI | CCGG | 6 cut(s) | 153, 342, 2318, 2546, 2591, 2806 |
| BslFI | GGGAC | 1 cut(s) | 395 |
| BsmAI | GTCTC | 3 cut(s) | 209, 1838, 3026 |
| BsmFI | GGGAC | 1 cut(s) | 395 |
| BsmI | GAATGC | 1 cut(s) | 3383 |
| Bso31I | GGTCTC | 2 cut(s) | 209, 3026 |
| BsoBI | CYCGRG | 1 cut(s) | 2226 |
| Bsp119I | TTCGAA | 1 cut(s) | 1557 |
| Bsp1286I | GDGCHC | 9 cut(s) | 659, 780, 1050, 1224, 1311, 1479, 1939, 2193, 2859 |
| Bsp19I | CCATGG | 1 cut(s) | 3021 |
| Bsp68I | TCGCGA | 1 cut(s) | 27 |
| BspACI | CCGC | 7 cut(s) | 8, 150, 165, 168, 225, 231, 237 |
| BspFNI | CGCG | 1 cut(s) | 27 |
| BspHI | TCATGA | 4 cut(s) | 640, 1456, 2065, 3028 |
| BspLI | GGNNCC | 3 cut(s) | 133, 411, 1134 |
| BspMAI | CTGCAG | 2 cut(s) | 1972, 3313 |
| BspPI | GGATC | 7 cut(s) | 341, 482, 583, 588, 1528, 3189, 3207 |
| BspT104I | TTCGAA | 1 cut(s) | 1557 |
| BspTI | CTTAAG | 1 cut(s) | 3115 |
| BspTNI | GGTCTC | 2 cut(s) | 209, 3026 |
| BsrDI | GCAATG | 3 cut(s) | 629, 1137, 1224 |
| BsrFI | RCCGGY | 3 cut(s) | 2317, 2545, 2590 |
| BsrI | ACTGG | 1 cut(s) | 1583 |
| BssAI | RCCGGY | 3 cut(s) | 2317, 2545, 2590 |
| BssECI | CCNNGG | 6 cut(s) | 51, 114, 402, 597, 669, 3021 |
| BssNI | GRCGYC | 2 cut(s) | 162, 912 |
| BssT1I | CCWWGG | 4 cut(s) | 51, 114, 669, 3021 |
| Bst2UI | CCWGG | 1 cut(s) | 2168 |
| Bst4CI | ACNGT | 4 cut(s) | 1634, 1750, 2440, 2659 |
| Bst6I | CTCTTC | 4 cut(s) | 252, 1455, 1671, 2883 |
| BstACI | GRCGYC | 2 cut(s) | 162, 912 |
| BstAFI | CTTAAG | 1 cut(s) | 3115 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1751 |
| BstBAI | YACGTR | 1 cut(s) | 3337 |
| BstBI | TTCGAA | 1 cut(s) | 1557 |
| BstC8I | GCNNGC | 8 cut(s) | 521, 525, 2445, 2449, 2547, 2592, 2596, 2772 |
| BstDSI | CCRYGG | 2 cut(s) | 597, 3021 |
| BstF5I | GGATG | 5 cut(s) | 446, 1192, 2815, 2974, 3133 |
| BstFNI | CGCG | 1 cut(s) | 27 |
| BstHHI | GCGC | 1 cut(s) | 3229 |
| BstMAI | GTCTC | 3 cut(s) | 209, 1838, 3026 |
| BstNI | CCWGG | 1 cut(s) | 2168 |
| BstNSI | RCATGY | 2 cut(s) | 2342, 2774 |
| BstSCI | CCNGG | 2 cut(s) | 2166, 2805 |
| BstSFI | CTRYAG | 4 cut(s) | 414, 1968, 3309, 3344 |
| BstSLI | GKGCMC | 1 cut(s) | 1939 |
| BstSNI | TACGTA | 1 cut(s) | 3337 |
| BstUI | CGCG | 1 cut(s) | 27 |
| BstX2I | RGATCY | 3 cut(s) | 575, 2220, 3181 |
| BstYI | RGATCY | 3 cut(s) | 575, 2220, 3181 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1800 |
| BtgI | CCRYGG | 2 cut(s) | 597, 3021 |
| BtgZI | GCGATG | 3 cut(s) | 8, 42, 141 |
| BtsCI | GGATG | 5 cut(s) | 446, 1192, 2815, 2974, 3133 |
| BtsIMutI | CAGTG | 4 cut(s) | 91, 1597, 2625, 2655 |
| BtuMI | TCGCGA | 1 cut(s) | 27 |
| Cac8I | GCNNGC | 8 cut(s) | 521, 525, 2445, 2449, 2547, 2592, 2596, 2772 |
| CaiI | CAGNNNCTG | 6 cut(s) | 356, 1847, 2351, 2459, 2498, 2645 |
| CciI | TCATGA | 4 cut(s) | 640, 1456, 2065, 3028 |
| CfoI | GCGC | 1 cut(s) | 3229 |
| Cfr10I | RCCGGY | 3 cut(s) | 2317, 2545, 2590 |
| Cfr13I | GGNCC | 3 cut(s) | 409, 1595, 2841 |
| CseI | GACGC | 4 cut(s) | 170, 2078, 2096, 2525 |
| Csp6I | GTAC | 4 cut(s) | 558, 1718, 2504, 3338 |
| CspAI | ACCGGT | 1 cut(s) | 2317 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 208, 243, 2282, 2317 |
| CviQI | GTAC | 4 cut(s) | 558, 1718, 2504, 3338 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1243 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1243 |
| EaeI | YGGCCR | 9 cut(s) | 589, 730, 817, 865, 1000, 1087, 1174, 1261, 1348 |
| Eam1104I | CTCTTC | 4 cut(s) | 252, 1455, 1671, 2883 |
| EarI | CTCTTC | 4 cut(s) | 252, 1455, 1671, 2883 |
| EciI | GGCGGA | 1 cut(s) | 23 |
| Eco105I | TACGTA | 1 cut(s) | 3337 |
| Eco130I | CCWWGG | 4 cut(s) | 51, 114, 669, 3021 |
| Eco147I | AGGCCT | 1 cut(s) | 2707 |
| Eco24I | GRGCYC | 6 cut(s) | 659, 780, 1050, 1224, 1479, 2193 |
| Eco31I | GGTCTC | 2 cut(s) | 209, 3026 |
| Eco32I | GATATC | 1 cut(s) | 1288 |
| Eco47I | GGWCC | 1 cut(s) | 409 |
| Eco57I | CTGAAG | 8 cut(s) | 353, 1449, 1530, 1695, 2520, 2907, 3104, 3206 |
| Eco81I | CCTNAGG | 1 cut(s) | 1800 |
| Eco88I | CYCGRG | 1 cut(s) | 2226 |
| EcoO109I | RGGNCCY | 1 cut(s) | 409 |
| EcoRII | CCWGG | 1 cut(s) | 2166 |
| EcoRV | GATATC | 1 cut(s) | 1288 |
| EcoT14I | CCWWGG | 4 cut(s) | 51, 114, 669, 3021 |
| EcoT22I | ATGCAT | 1 cut(s) | 631 |
| EcoT38I | GRGCYC | 6 cut(s) | 659, 780, 1050, 1224, 1479, 2193 |
| ErhI | CCWWGG | 4 cut(s) | 51, 114, 669, 3021 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 3201, 3233 |
| FaqI | GGGAC | 1 cut(s) | 395 |
| FauNDI | CATATG | 1 cut(s) | 3009 |
| FbaI | TGATCA | 1 cut(s) | 3097 |
| FokI | GGATG | 5 cut(s) | 433, 1199, 2822, 2981, 3140 |
| FriOI | GRGCYC | 6 cut(s) | 659, 780, 1050, 1224, 1479, 2193 |
| FspBI | CTAG | 4 cut(s) | 2060, 2082, 2325, 2733 |
| GlaI | GCGC | 1 cut(s) | 3228 |
| HapII | CCGG | 6 cut(s) | 153, 342, 2318, 2546, 2591, 2806 |
| HgaI | GACGC | 4 cut(s) | 170, 2078, 2096, 2525 |
| HhaI | GCGC | 1 cut(s) | 3229 |
| Hin1I | GRCGYC | 2 cut(s) | 162, 912 |
| Hin6I | GCGC | 1 cut(s) | 3227 |
| HinP1I | GCGC | 1 cut(s) | 3227 |
| HincII | GTYRAC | 3 cut(s) | 333, 444, 2250 |
| HindII | GTYRAC | 3 cut(s) | 333, 444, 2250 |
| HindIII | AAGCTT | 1 cut(s) | 1738 |
| HpaII | CCGG | 6 cut(s) | 153, 342, 2318, 2546, 2591, 2806 |
| HphI | GGTGA | 7 cut(s) | 139, 170, 356, 497, 851, 2428, 2575 |
| Hpy166II | GTNNAC | 5 cut(s) | 333, 444, 809, 1904, 2250 |
| Hpy8I | GTNNAC | 5 cut(s) | 333, 444, 809, 1904, 2250 |
| Hpy99I | CGWCG | 2 cut(s) | 125, 2928 |
| HpyAV | CCTTC | 4 cut(s) | 307, 2666, 3040, 3184 |
| HpyCH4III | ACNGT | 4 cut(s) | 1634, 1750, 2440, 2659 |
| HpyCH4IV | ACGT | 3 cut(s) | 912, 1716, 3336 |
| HpySE526I | ACGT | 3 cut(s) | 912, 1716, 3336 |
| Hsp92I | GRCGYC | 2 cut(s) | 162, 912 |
| HspAI | GCGC | 1 cut(s) | 3227 |
| KroI | GCCGGC | 2 cut(s) | 2545, 2590 |
| KroNI | GCCGGC | 2 cut(s) | 2547, 2592 |
| Ksp22I | TGATCA | 1 cut(s) | 3097 |
| LmnI | GCTCC | 3 cut(s) | 137, 1306, 2854 |
| MaeI | CTAG | 4 cut(s) | 2060, 2082, 2325, 2733 |
| MaeII | ACGT | 3 cut(s) | 912, 1716, 3336 |
| MfeI | CAATTG | 2 cut(s) | 1767, 2568 |
| MflI | RGATCY | 3 cut(s) | 575, 2220, 3181 |
| MhlI | GDGCHC | 9 cut(s) | 659, 780, 1050, 1224, 1311, 1479, 1939, 2193, 2859 |
| MlsI | TGGCCA | 6 cut(s) | 732, 1002, 1089, 1176, 1263, 1350 |
| MluNI | TGGCCA | 6 cut(s) | 732, 1002, 1089, 1176, 1263, 1350 |
| MmeI | TCCRAC | 2 cut(s) | 228, 1554 |
| Mox20I | TGGCCA | 6 cut(s) | 732, 1002, 1089, 1176, 1263, 1350 |
| Mph1103I | ATGCAT | 1 cut(s) | 631 |
| MroNI | GCCGGC | 2 cut(s) | 2545, 2590 |
| MscI | TGGCCA | 6 cut(s) | 732, 1002, 1089, 1176, 1263, 1350 |
| MslI | CAYNNNNRTG | 2 cut(s) | 27, 1455 |
| Msp20I | TGGCCA | 6 cut(s) | 732, 1002, 1089, 1176, 1263, 1350 |
| MspA1I | CMGCKG | 5 cut(s) | 563, 698, 1949, 2261, 3082 |
| MspCI | CTTAAG | 1 cut(s) | 3115 |
| MspI | CCGG | 6 cut(s) | 153, 342, 2318, 2546, 2591, 2806 |
| MspR9I | CCNGG | 2 cut(s) | 2168, 2807 |
| MunI | CAATTG | 2 cut(s) | 1767, 2568 |
| Mva1269I | GAATGC | 1 cut(s) | 3383 |
| MvaI | CCWGG | 1 cut(s) | 2168 |
| MvnI | CGCG | 1 cut(s) | 27 |
| NaeI | GCCGGC | 2 cut(s) | 2547, 2592 |
| NciI | CCSGG | 1 cut(s) | 2807 |
| NcoI | CCATGG | 1 cut(s) | 3021 |
| NdeI | CATATG | 1 cut(s) | 3009 |
| NgoMIV | GCCGGC | 2 cut(s) | 2545, 2590 |
| NlaIV | GGNNCC | 3 cut(s) | 133, 411, 1134 |
| NmeAIII | GCCGAG | 3 cut(s) | 427, 1911, 2044 |
| NmuCI | GTSAC | 7 cut(s) | 145, 158, 2084, 2434, 2581, 3068, 3235 |
| NruI | TCGCGA | 1 cut(s) | 27 |
| NsiI | ATGCAT | 1 cut(s) | 631 |
| NspI | RCATGY | 2 cut(s) | 2342, 2774 |
| NspV | TTCGAA | 1 cut(s) | 1557 |
| PaeI | GCATGC | 1 cut(s) | 2774 |
| PaeR7I | CTCGAG | 1 cut(s) | 2226 |
| PagI | TCATGA | 4 cut(s) | 640, 1456, 2065, 3028 |
| PceI | AGGCCT | 1 cut(s) | 2707 |
| PctI | GAATGC | 1 cut(s) | 3383 |
| PdiI | GCCGGC | 2 cut(s) | 2547, 2592 |
| PflMI | CCANNNNNTGG | 9 cut(s) | 739, 826, 874, 922, 1009, 1096, 1183, 1270, 1357 |
| PinAI | ACCGGT | 1 cut(s) | 2317 |
| Ppu21I | YACGTR | 1 cut(s) | 3337 |
| PpuMI | RGGWCCY | 1 cut(s) | 409 |
| PshBI | ATTAAT | 6 cut(s) | 795, 978, 1065, 1326, 2412, 2559 |
| Psp5II | RGGWCCY | 1 cut(s) | 409 |
| Psp6I | CCWGG | 1 cut(s) | 2166 |
| PspGI | CCWGG | 1 cut(s) | 2166 |
| PspN4I | GGNNCC | 3 cut(s) | 133, 411, 1134 |
| PspPI | GGNCC | 3 cut(s) | 409, 1595, 2841 |
| PspPPI | RGGWCCY | 1 cut(s) | 409 |
| PstI | CTGCAG | 2 cut(s) | 1972, 3313 |
| PstNI | CAGNNNCTG | 6 cut(s) | 356, 1847, 2351, 2459, 2498, 2645 |
| PsuI | RGATCY | 3 cut(s) | 575, 2220, 3181 |
| PvuII | CAGCTG | 5 cut(s) | 563, 698, 1949, 2261, 3082 |
| RruI | TCGCGA | 1 cut(s) | 27 |
| RsaI | GTAC | 4 cut(s) | 559, 1719, 2505, 3339 |
| RsaNI | GTAC | 4 cut(s) | 558, 1718, 2504, 3338 |
| RseI | CAYNNNNRTG | 2 cut(s) | 27, 1455 |
| Sau96I | GGNCC | 3 cut(s) | 409, 1595, 2841 |
| ScrFI | CCNGG | 2 cut(s) | 2168, 2807 |
| SduI | GDGCHC | 9 cut(s) | 659, 780, 1050, 1224, 1311, 1479, 1939, 2193, 2859 |
| SfcI | CTRYAG | 4 cut(s) | 414, 1968, 3309, 3344 |
| Sfr274I | CTCGAG | 1 cut(s) | 2226 |
| SfuI | TTCGAA | 1 cut(s) | 1557 |
| SinI | GGWCC | 1 cut(s) | 409 |
| SlaI | CTCGAG | 1 cut(s) | 2226 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 27, 1455 |
| SmlI | CTYRAG | 6 cut(s) | 2050, 2226, 2282, 2912, 3115, 3240 |
| SmoI | CTYRAG | 6 cut(s) | 2050, 2226, 2282, 2912, 3115, 3240 |
| SnaBI | TACGTA | 1 cut(s) | 3337 |
| SphI | GCATGC | 1 cut(s) | 2774 |
| SseBI | AGGCCT | 1 cut(s) | 2707 |
| SsiI | CCGC | 7 cut(s) | 8, 150, 165, 168, 225, 231, 237 |
| SspI | AATATT | 2 cut(s) | 1095, 1269 |
| SspMI | CTAG | 4 cut(s) | 2060, 2082, 2325, 2733 |
| StuI | AGGCCT | 1 cut(s) | 2707 |
| StyD4I | CCNGG | 2 cut(s) | 2166, 2805 |
| StyI | CCWWGG | 4 cut(s) | 51, 114, 669, 3021 |
| TaaI | ACNGT | 4 cut(s) | 1634, 1750, 2440, 2659 |
| TaiI | ACGT | 3 cut(s) | 915, 1719, 3339 |
| TaqI | TCGA | 2 cut(s) | 1557, 2227 |
| TatI | WGTACW | 1 cut(s) | 557 |
| TauI | GCSGC | 2 cut(s) | 167, 170 |
| TscAI | CASTG | 4 cut(s) | 91, 1597, 2632, 2662 |
| TseFI | GTSAC | 7 cut(s) | 145, 158, 2084, 2434, 2581, 3068, 3235 |
| Tsp45I | GTSAC | 7 cut(s) | 145, 158, 2084, 2434, 2581, 3068, 3235 |
| TspGWI | ACGGA | 2 cut(s) | 586, 1533 |
| TspRI | CASTG | 4 cut(s) | 91, 1597, 2632, 2662 |
| Van91I | CCANNNNNTGG | 9 cut(s) | 739, 826, 874, 922, 1009, 1096, 1183, 1270, 1357 |
| Vha464I | CTTAAG | 1 cut(s) | 3115 |
| VpaK11BI | GGWCC | 1 cut(s) | 409 |
| VspI | ATTAAT | 6 cut(s) | 795, 978, 1065, 1326, 2412, 2559 |
| XapI | RAATTY | 3 cut(s) | 1963, 1996, 2867 |
| XceI | RCATGY | 2 cut(s) | 2342, 2774 |
| XhoI | CTCGAG | 1 cut(s) | 2226 |
| XspI | CTAG | 4 cut(s) | 2060, 2082, 2325, 2733 |
| ZraI | GACGTC | 1 cut(s) | 913 |
| Zsp2I | ATGCAT | 1 cut(s) | 631 |