Rorug07G0168900

single fertilization

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0013480
Physical Location & Seq
Reverse (-)
13651831 .. 13656887
5057 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0168900.1

Sequence Viewer

Length: 3135 bp
ATGTTCAAGTCGGCGAGATGGAGAAGCGACAAGAACAAGATCAAGACTCTGTTCAAATTGCAGTTTCATGCAACTCAGGTGCCAAAATTGGGTGTGGAGGCATTGATGGTATCTGTGATTCCTGGAGATGTGGGAAAGCCAACTGTGAAATTAGATAAAGCTGTGGTTCGAGATGGGAGCTGTAAATGGGAAAATCCGGTTTATGAAACGGTTAAATTCGACCGGGAGCCAAGAACCGGGAAGATCAAGGAGAGACTCTATAGTTTTGTCCTTTCAACTGGATCTTCTAAAGCAAGTGTTCTTGGGGAGGTTTCTGTTGATTTTGCCGAGTATTCTGAGGCTACTAAAGCTTCCTCTGTGTCTCTTCCTCTTAAGAATTCAAGTGCAGTATTGCATGTTTCGATTCAAAAGTTACAGGCAAATGCTGATCAGAGAGAGGTAGAAGGCTGTGAAGATGCCAAAGTTAAATCTCAGGATAATAGCTTGATGAGACACTTGAGCAATAATGATGCAGATGAAAGCGTTTTAGTGGATGAGACGATCAATAGGACCACTCAAAATGCTGAGTGCAACCGCACGGCCTCTATTGGATCAGACATTACATTGTCCAGTTCTGACAGCAGCTCTGGACTCGATACTTCTCGAGAGCTTGTACTGAGAAACATTAACAATTGTCACGACCCTTCCAACTATCTATCATCACCAAATCAGCCCTCAATACCACACAGACCTGGTGTTTATGGCTCGACAAATAATGAGCAACAGCAATCACAGTGGGAGTGGTCTGCAGATTCTGAACATGGAGTAAGTAGTGATGGTTCAACAAAGAGTTCTCAGGACAACCTTCTGAGAGAAAGGTCTCTGCAGTCTTCAGGCGACGATATTGATAAGCTAAAGGCTGAGCTTGTTGTTTTGTCCAGACAAGCGGACATGTCAGAGTTAGAGTTACAAACACTAAGGAAGCAGATAGTAAAAGAGAGCAAAAGGGGGCATGATCTTTCAAGAGAAGTCGTTAGCTTGAAAGAGGAACGAGATGCTTTCAAGGCTGAATGTGAGAAGCTAAAAGCCTTTCAGTATCGGATGGATGATACAAAAACAAAAAACAGGTTCCAGTTAGAAGGTGGGGATCTACGGGCTCTTGTTGATGAAATCAGACAAGAACTCAGCTGTGAGAAGGACCTGAATAGCAATCTTCGGTTACAGCTCCAGAAGACCCAAGAGTCAAATGCTGAGTTGATTCTTGCTGTACGAGACCTGGAGGAAATATTGGAGCAGAAGAATGGTGAAGCTGCTAATTCCAACAGATCAGAATCCACAAATGATGCTGCAGGTTCGAGAGCAACCAACTCAAATGATGTGGAAACTGAGGATGAGGAGCAGAAGGAACTGGAGGATCTTGTTAAGGAGCACAGCCATGCCAAGGATACACACCTGCTGGAGAAACAGATTACAGACCTCTATAATGAATTAGAGATCTACAAGAGAGACAAAGATGAGCTTGAGATGCAAATGGAGCAGCTTGCTCTTGACTATGAGATATTGAAACAGGAAAACCATGACATCTCATACAAGCTAGAGCAAAGCACACTGCAAGAACAACTCAAGATGCAGTATGAATGCTCATCTCCCACTGCTAGTGTAAATGAGCTTCATTACCAAATTGAGGATCTAGAAACAGAATTGAAGAAGCAGGCCGAAGATTTTTCTAATTCGTTGGCTACCATAAAGGAACTTCAATCTCACATCAAAAGTATGGAGGAAGAACTAGAGAAGCAGGCACAAGGATTTGAAGCTGATTTAGAAACTGTAACATGTGCTAAAGTTGAGCAGGAGCAAAGAGCCATCCGAGCAGAGGAGGTACTGCGGAAAACTAGATTGAAAAATGCGAATACAGCTGAGAGGCTTCAGGAAGAATTTAGACGACTTTCTTCACAAATGGCCTCCACATTTGATGCGAATGAGAAGGTAGCAATGAAAGCAATGACAGAAGCGAGTGAACTGGGTGCACAGAAGAGTCACCTTGAAGAATTGCTCAAGAAAACTAAGGAAGAGCTCCAGGCAAGTAGGGATGAGTATGAGGCAAAATTGCAGAAGCTTTCCAACGAACTAGATGAGAAAACCAGAGAGATGGAACAGATGTTATTGGAAATCCAGAATAAGTCCATGCAGCTTGAAGATCAGCAGAAGCAAGAGGGAGATTTCTCTGAGGTGATTTTACAGCTCAAAGCTGAGATTGAAAGGCTTACAAGAGAAAATAACAGTCTTTCAGAGGAAGTAGAGCAAAACAAAATTTCGAGTGCTGAGTTGGAGAAAATGAAGAAATCAATTGAGGAAACTGAGATGCTGATTCAAAGAGGAAATGTAGAAAGGAATGAGTTGGTGAGTACAATTGATATGCTGAAGAAGGAAGCAGAGAAGTCGTTGGAGAAGTTGAATAAAATGAAGTATCTTATGGATGAAAAAGAGGCAACCATTCGACGCCTACAATTGGAGTCTGACAAGCTGAAAGCTCAGTGTAATGACTTGAAACGTACTCTCTCTGAGGATGAGGTAGAGAAAGGAAGTCTTAAAAGGCAGGTATTTGATCTTAAGGCTGATCTGAAGAAGAAGGAGGATGCACTCACTACTATTGAAAAGAAGCTCAAGGATAGCAATGGACGAACAATAGTTTCAGATGGAGCTAAACATAATCTGAGAAACAATAAGTCTCTGCCTGTTCCTCGTGCAACAAAAGAAGTTGCAAGTCTGAGGGAGAGAATAAAACTGCTGGAGGGACAAATAAAGTTAAAGGAAGCTGCTTTGGAACGCTCAACTGCTTCTTTTCTGGAGAAGGAAAAGGATCTTCAGAATGTAATCGAAGAGTTAGAAATCAGGGTGGAAGAAATCAATCAAAACTGTGCATTTGAACAGGATATTACTTCAAAAGATAATATGCTTGAAGAAGTGAGGCGAGTGTCTGATAACTTATCAACTGAATTGGCATCACTAAAGGAGAGAAATATGTCAATGGAAAGTGAACTAAAAGAGATGCAGGAGAGATATTCAGAGATAAGTCTCAAATTTGCAGAGGTAGAAGGTGAAAGACAACAGCTTGTAATGACGGTACGCAACCTAAAAAATTCCAAGAGGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1044

Amino Acids

119.35

Weight (kDa)

5.09

Isoelectric Point (pI)

48.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NT-C2 PF10358 11 - 143 1.3e-21 N-terminal C2 in EEIG1 and EHBP1 proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013480)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20970
fragaria_vesca FvH4_5g26490 FvH4_5g26490
malus_domestica MD06G1001600.v1.1
prunus_persica Prupe.5G002400_v2.0.a1 Prupe.5G002400_v2.0.a1
pyrus_communis pycom06g00170 pycom16g26270 pycom16g26280
rosa_chinensis RchiOBHm_Chr7g0217401
rosa_laevigata RLG00000002480
rosa_roxburghii Rroxscaffold_3G00242210
rosa_rugosa Rorug07G0168900
rosa_samantha Rh7AG308500 Rh7BG299200 Rh7CG327800 Rh7DG307800
rosa_wichuraiana Rw7G026160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1440
AasI GACNNNNNNGTC 1 cut(s) 1219
Acc36I ACCTGC 3 cut(s) 1319, 1440, 2566
AccB1I GGYRCC 1 cut(s) 79
AciI CCGC 3 cut(s) 574, 926, 1864
AclWI GGATC 6 cut(s) 289, 598, 1134, 1401, 1674, 2847
AcsI RAATTY 6 cut(s) 215, 376, 1913, 2289, 3059, 3118
AcuI CTGAAG 5 cut(s) 855, 1889, 2420, 2621, 2828
AcyI GRCGYC 1 cut(s) 2479
AfaI GTAC 6 cut(s) 654, 1248, 1860, 2386, 2533, 3105
AfiI CCNNNNNNNGG 6 cut(s) 89, 236, 1420, 1663, 1852, 2488
AflII CTTAAG 2 cut(s) 371, 2588
AflIII ACRYGT 2 cut(s) 930, 1811
AjnI CCWGG 4 cut(s) 121, 730, 1254, 2055
Alw21I GWGCWC 3 cut(s) 1410, 2008, 2055
Alw26I GTCTC 9 cut(s) 247, 366, 484, 530, 864, 1245, 1479, 2712, 3059
Alw44I GTGCAC 1 cut(s) 2004
AlwI GGATC 6 cut(s) 289, 598, 1134, 1401, 1674, 2847
AlwNI CAGNNNCTG 1 cut(s) 794
Ama87I CYCGRG 1 cut(s) 642
AoxI GGCC 3 cut(s) 579, 1692, 1938
ApaLI GTGCAC 1 cut(s) 2004
ApeKI GCWGC 6 cut(s) 621, 1289, 1325, 1516, 2167, 2795
ApoI RAATTY 6 cut(s) 215, 376, 1913, 2289, 3059, 3118
AspS9I GGNCC 2 cut(s) 549, 1177
AsuC2I CCSGG 2 cut(s) 224, 238
AsuHPI GGTGA 6 cut(s) 693, 1295, 2009, 2221, 2392, 3089
AvaI CYCGRG 1 cut(s) 642
AvaII GGWCC 2 cut(s) 549, 1177
BaeGI GKGCMC 1 cut(s) 2008
BanI GGYRCC 1 cut(s) 79
BanII GRGCYC 2 cut(s) 1138, 2055
BarI GAAGNNNNNNTAC 2 cut(s) 1632, 1664
BauI CACGAG 1 cut(s) 2721
BbsI GAAGAC 2 cut(s) 861, 1217
Bbv12I GWGCWC 3 cut(s) 1410, 2008, 2055
BbvI GCAGC 6 cut(s) 633, 1276, 1312, 1528, 2179, 2782
BccI CCATC 8 cut(s) 12, 100, 167, 809, 1075, 1850, 2122, 2669
BceAI ACGGC 1 cut(s) 594
BcgI CGANNNNNNTGC 2 cut(s) 2400, 2434
BciT130I CCWGG 4 cut(s) 123, 732, 1256, 2057
BciVI GTATCC 1 cut(s) 1417
BclI TGATCA 1 cut(s) 427
BcnI CCSGG 2 cut(s) 224, 238
BcoDI GTCTC 9 cut(s) 247, 366, 484, 530, 864, 1245, 1479, 2712, 3059
BfaI CTAG 7 cut(s) 1574, 1635, 1670, 1766, 1872, 2108, 3133
BfmI CTRYAG 4 cut(s) 259, 786, 863, 1326
BfrI CTTAAG 2 cut(s) 371, 2588
BfuAI ACCTGC 3 cut(s) 1319, 1440, 2566
BfuI GTATCC 1 cut(s) 1417
BglII AGATCT 1 cut(s) 1473
BisI GCNGC 6 cut(s) 622, 1290, 1326, 1517, 2168, 2796
BlpI GCTNAGC 1 cut(s) 900
BlsI GCNGC 6 cut(s) 623, 1291, 1327, 1518, 2169, 2797
Bme1390I CCNGG 6 cut(s) 123, 224, 238, 732, 1256, 2057
Bme18I GGWCC 2 cut(s) 549, 1177
BmeT110I CYCGRG 1 cut(s) 642
BmgT120I GGNCC 2 cut(s) 549, 1177
BmiI GGNNCC 3 cut(s) 81, 228, 1109
BmrFI CCNGG 6 cut(s) 123, 224, 238, 732, 1256, 2057
BmrI ACTGGG 1 cut(s) 2009
BmuI ACTGGG 1 cut(s) 2009
BpiI GAAGAC 2 cut(s) 861, 1217
BpmI CTGGAG 8 cut(s) 144, 1190, 1277, 1409, 1457, 2039, 2789, 2846
Bpu1102I GCTNAGC 1 cut(s) 900
BpuEI CTTGAG 5 cut(s) 517, 1520, 1586, 2018, 2627
BpuMI CCSGG 2 cut(s) 224, 238
BsaBI GATNNNNATC 1 cut(s) 1309
BsaHI GRCGYC 1 cut(s) 2479
BsaI GGTCTC 2 cut(s) 864, 1245
BsaJI CCNNGG 1 cut(s) 1419
BsaWI WCCGGW 1 cut(s) 196
Bsc4I CCNNNNNNNGG 6 cut(s) 89, 236, 1420, 1663, 1852, 2488
Bse1I ACTGG 5 cut(s) 283, 609, 1111, 1392, 2004
Bse3DI GCAATG 3 cut(s) 1977, 1986, 2659
Bse8I GATNNNNATC 1 cut(s) 1309
BseBI CCWGG 4 cut(s) 123, 732, 1256, 2057
BseDI CCNNGG 1 cut(s) 1419
BseGI GGATG 9 cut(s) 538, 1086, 1090, 1375, 1842, 2074, 2461, 2551, 2620
BseJI GATNNNNATC 1 cut(s) 1309
BseLI CCNNNNNNNGG 6 cut(s) 89, 236, 1420, 1663, 1852, 2488
BseMI GCAATG 3 cut(s) 1977, 1986, 2659
BseNI ACTGG 5 cut(s) 283, 609, 1111, 1392, 2004
BseRI GAGGAG 2 cut(s) 1388, 1868
BseSI GKGCMC 1 cut(s) 2008
BseXI GCAGC 6 cut(s) 633, 1276, 1312, 1528, 2179, 2782
BsgI GTGCAG 1 cut(s) 405
Bsh1285I CGRYCG 1 cut(s) 223
BshFI GGCC 3 cut(s) 581, 1694, 1940
BshNI GGYRCC 1 cut(s) 79
BsiEI CGRYCG 1 cut(s) 223
BsiHKAI GWGCWC 3 cut(s) 1410, 2008, 2055
BsiHKCI CYCGRG 1 cut(s) 642
BsiSI CCGG 3 cut(s) 197, 223, 237
BslFI GGGAC 1 cut(s) 2787
BslI CCNNNNNNNGG 6 cut(s) 89, 236, 1420, 1663, 1852, 2488
BsmAI GTCTC 9 cut(s) 247, 366, 484, 530, 864, 1245, 1479, 2712, 3059
BsmBI CGTCTC 1 cut(s) 530
BsmFI GGGAC 1 cut(s) 2787
BsmI GAATGC 1 cut(s) 1622
BsnI GGCC 3 cut(s) 581, 1694, 1940
Bso31I GGTCTC 2 cut(s) 864, 1245
BsoBI CYCGRG 1 cut(s) 642
Bsp1286I GDGCHC 4 cut(s) 1138, 1410, 2008, 2055
Bsp1720I GCTNAGC 1 cut(s) 900
BspACI CCGC 3 cut(s) 574, 926, 1864
BspANI GGCC 3 cut(s) 581, 1694, 1940
BspLI GGNNCC 3 cut(s) 81, 228, 1109
BspMAI CTGCAG 3 cut(s) 790, 867, 1330
BspMI ACCTGC 3 cut(s) 1319, 1440, 2566
BspPI GGATC 6 cut(s) 289, 598, 1134, 1401, 1674, 2847
BspQI GCTCTTC 1 cut(s) 2043
BspT107I GGYRCC 1 cut(s) 79
BspTI CTTAAG 2 cut(s) 371, 2588
BspTNI GGTCTC 2 cut(s) 864, 1245
BsrDI GCAATG 3 cut(s) 1977, 1986, 2659
BsrI ACTGG 5 cut(s) 283, 609, 1111, 1392, 2004
BssECI CCNNGG 1 cut(s) 1419
BssNI GRCGYC 1 cut(s) 2479
BssSI CACGAG 1 cut(s) 2721
BssT1I CCWWGG 1 cut(s) 1419
Bst2BI CACGAG 1 cut(s) 2721
Bst2UI CCWGG 4 cut(s) 123, 732, 1256, 2057
Bst4CI ACNGT 7 cut(s) 145, 211, 774, 1807, 2261, 2897, 3103
Bst6I CTCTTC 4 cut(s) 369, 2006, 2043, 2853
BstACI GRCGYC 1 cut(s) 2479
BstAFI CTTAAG 2 cut(s) 371, 2588
BstC8I GCNNGC 3 cut(s) 1521, 1692, 1776
BstF5I GGATG 9 cut(s) 538, 1086, 1090, 1375, 1842, 2074, 2461, 2551, 2620
BstMAI GTCTC 9 cut(s) 247, 366, 484, 530, 864, 1245, 1479, 2712, 3059
BstMCI CGRYCG 1 cut(s) 223
BstMWI GCNNNNNNNGC 7 cut(s) 347, 1043, 1504, 1513, 1847, 1892, 1976
BstNI CCWGG 4 cut(s) 123, 732, 1256, 2057
BstNSI RCATGY 3 cut(s) 398, 934, 1815
BstSCI CCNGG 6 cut(s) 121, 222, 236, 730, 1254, 2055
BstSFI CTRYAG 4 cut(s) 259, 786, 863, 1326
BstSLI GKGCMC 1 cut(s) 2008
BstV1I GCAGC 6 cut(s) 633, 1276, 1312, 1528, 2179, 2782
BstV2I GAAGAC 2 cut(s) 861, 1217
BstX2I RGATCY 6 cut(s) 281, 1126, 1393, 1473, 1666, 2839
BstXI CCANNNNNNTGG 1 cut(s) 2128
BstYI RGATCY 6 cut(s) 281, 1126, 1393, 1473, 1666, 2839
BsuI GTATCC 1 cut(s) 1417
BsuRI GGCC 3 cut(s) 581, 1694, 1940
BtsCI GGATG 9 cut(s) 538, 1086, 1090, 1375, 1842, 2074, 2461, 2551, 2620
BtsI GCAGTG 2 cut(s) 1586, 1629
BtsIMutI CAGTG 4 cut(s) 779, 1586, 1629, 2519
BveI ACCTGC 3 cut(s) 1319, 1440, 2566
Cac8I GCNNGC 3 cut(s) 1521, 1692, 1776
CaiI CAGNNNCTG 1 cut(s) 794
Cfr13I GGNCC 2 cut(s) 549, 1177
CseI GACGC 1 cut(s) 2487
CsiI ACCWGGT 1 cut(s) 730
Csp6I GTAC 6 cut(s) 653, 1247, 1859, 2385, 2532, 3104
CspCI CAANNNNNGTGG 4 cut(s) 755, 790, 1338, 1373
CviAII CATG 9 cut(s) 68, 395, 800, 931, 992, 1415, 1556, 1812, 2164
CviQI GTAC 6 cut(s) 653, 1247, 1859, 2385, 2532, 3104
DrdI GACNNNNNNGTC 1 cut(s) 1219
DseDI GACNNNNNNGTC 1 cut(s) 1219
Eam1104I CTCTTC 4 cut(s) 369, 2006, 2043, 2853
EarI CTCTTC 4 cut(s) 369, 2006, 2043, 2853
Ecl136II GAGCTC 1 cut(s) 2053
Eco130I CCWWGG 1 cut(s) 1419
Eco24I GRGCYC 2 cut(s) 1138, 2055
Eco31I GGTCTC 2 cut(s) 864, 1245
Eco47I GGWCC 2 cut(s) 549, 1177
Eco53kI GAGCTC 1 cut(s) 2053
Eco57I CTGAAG 5 cut(s) 855, 1889, 2420, 2621, 2828
Eco88I CYCGRG 1 cut(s) 642
EcoICRI GAGCTC 1 cut(s) 2053
EcoO109I RGGNCCY 1 cut(s) 1177
EcoRI GAATTC 1 cut(s) 376
EcoRII CCWGG 4 cut(s) 121, 730, 1254, 2055
EcoT14I CCWWGG 1 cut(s) 1419
EcoT38I GRGCYC 2 cut(s) 1138, 2055
ErhI CCWWGG 1 cut(s) 1419
Esp3I CGTCTC 1 cut(s) 530
FaeI CATG 9 cut(s) 71, 398, 803, 934, 995, 1418, 1559, 1815, 2167
FalI AAGNNNNNCTT 4 cut(s) 1482, 1514, 2550, 2582
FaqI GGGAC 1 cut(s) 2787
FatI CATG 9 cut(s) 67, 394, 799, 930, 991, 1414, 1555, 1811, 2163
FbaI TGATCA 1 cut(s) 427
Fnu4HI GCNGC 6 cut(s) 622, 1290, 1326, 1517, 2168, 2796
FokI GGATG 9 cut(s) 545, 1093, 1097, 1382, 1829, 2081, 2468, 2558, 2627
FriOI GRGCYC 2 cut(s) 1138, 2055
Fsp4HI GCNGC 6 cut(s) 622, 1290, 1326, 1517, 2168, 2796
FspBI CTAG 7 cut(s) 1574, 1635, 1670, 1766, 1872, 2108, 3133
GluI GCNGC 6 cut(s) 622, 1290, 1326, 1517, 2168, 2796
GsuI CTGGAG 8 cut(s) 144, 1190, 1277, 1409, 1457, 2039, 2789, 2846
HaeIII GGCC 3 cut(s) 581, 1694, 1940
HapII CCGG 3 cut(s) 197, 223, 237
HgaI GACGC 1 cut(s) 2487
Hin1I GRCGYC 1 cut(s) 2479
Hin1II CATG 9 cut(s) 71, 398, 803, 934, 995, 1418, 1559, 1815, 2167
HindIII AAGCTT 2 cut(s) 348, 2093
HpaII CCGG 3 cut(s) 197, 223, 237
HphI GGTGA 6 cut(s) 693, 1295, 2009, 2221, 2392, 3089
Hpy166II GTNNAC 3 cut(s) 1997, 2006, 3017
Hpy8I GTNNAC 3 cut(s) 1997, 2006, 3017
Hpy99I CGWCG 2 cut(s) 881, 2481
HpyCH4III ACNGT 7 cut(s) 145, 211, 774, 1807, 2261, 2897, 3103
HpyCH4IV ACGT 1 cut(s) 2530
HpyF10VI GCNNNNNNNGC 7 cut(s) 347, 1043, 1504, 1513, 1847, 1892, 1976
HpySE526I ACGT 1 cut(s) 2530
Hsp92I GRCGYC 1 cut(s) 2479
Hsp92II CATG 9 cut(s) 71, 398, 803, 934, 995, 1418, 1559, 1815, 2167
Ksp22I TGATCA 1 cut(s) 427
LguI GCTCTTC 1 cut(s) 2043
Lsp1109I GCAGC 6 cut(s) 633, 1276, 1312, 1528, 2179, 2782
MabI ACCWGGT 1 cut(s) 730
MaeI CTAG 7 cut(s) 1574, 1635, 1670, 1766, 1872, 2108, 3133
MaeII ACGT 1 cut(s) 2530
MaeIII GTNAC 6 cut(s) 411, 674, 945, 1197, 1807, 2015
MfeI CAATTG 4 cut(s) 670, 2325, 2388, 2486
MflI RGATCY 6 cut(s) 281, 1126, 1393, 1473, 1666, 2839
MhlI GDGCHC 4 cut(s) 1138, 1410, 2008, 2055
MlyI GAGTC 6 cut(s) 40, 249, 624, 1229, 2023, 2501
MmeI TCCRAC 5 cut(s) 711, 1323, 2124, 2286, 2403
MseI TTAA 8 cut(s) 213, 372, 465, 666, 1401, 2568, 2589, 2786
MslI CAYNNNNRTG 1 cut(s) 1413
MspA1I CMGCKG 2 cut(s) 1167, 1895
MspCI CTTAAG 2 cut(s) 371, 2588
MspI CCGG 3 cut(s) 197, 223, 237
MspR9I CCNGG 6 cut(s) 123, 224, 238, 732, 1256, 2057
MunI CAATTG 4 cut(s) 670, 2325, 2388, 2486
Mva1269I GAATGC 1 cut(s) 1622
MvaI CCWGG 4 cut(s) 123, 732, 1256, 2057
MwoI GCNNNNNNNGC 7 cut(s) 347, 1043, 1504, 1513, 1847, 1892, 1976
NciI CCSGG 2 cut(s) 224, 238
NlaIII CATG 9 cut(s) 71, 398, 803, 934, 995, 1418, 1559, 1815, 2167
NlaIV GGNNCC 3 cut(s) 81, 228, 1109
NmeAIII GCCGAG 1 cut(s) 352
NmuCI GTSAC 2 cut(s) 674, 2015
NspI RCATGY 3 cut(s) 398, 934, 1815
PaeR7I CTCGAG 1 cut(s) 642
PaqCI CACCTGC 1 cut(s) 1440
PciI ACATGT 2 cut(s) 930, 1811
PciSI GCTCTTC 1 cut(s) 2043
PctI GAATGC 1 cut(s) 1622
PfeI GAWTC 6 cut(s) 118, 403, 791, 1237, 1310, 2347
PfoI TCCNGGA 1 cut(s) 121
PkrI GCNGC 6 cut(s) 623, 1291, 1327, 1518, 2169, 2797
PleI GAGTC 6 cut(s) 40, 249, 624, 1228, 2022, 2500
PpsI GAGTC 6 cut(s) 40, 249, 624, 1228, 2022, 2500
PpuMI RGGWCCY 1 cut(s) 1177
PscI ACATGT 2 cut(s) 930, 1811
Psp124BI GAGCTC 1 cut(s) 2055
Psp5II RGGWCCY 1 cut(s) 1177
Psp6I CCWGG 4 cut(s) 121, 730, 1254, 2055
PspGI CCWGG 4 cut(s) 121, 730, 1254, 2055
PspN4I GGNNCC 3 cut(s) 81, 228, 1109
PspPI GGNCC 2 cut(s) 549, 1177
PspPPI RGGWCCY 1 cut(s) 1177
PsrI GAACNNNNNNTAC 2 cut(s) 802, 834
PstI CTGCAG 3 cut(s) 790, 867, 1330
PstNI CAGNNNCTG 1 cut(s) 794
PsuI RGATCY 6 cut(s) 281, 1126, 1393, 1473, 1666, 2839
PvuII CAGCTG 2 cut(s) 1167, 1895
RsaI GTAC 6 cut(s) 654, 1248, 1860, 2386, 2533, 3105
RsaNI GTAC 6 cut(s) 653, 1247, 1859, 2385, 2532, 3104
RseI CAYNNNNRTG 1 cut(s) 1413
SacI GAGCTC 1 cut(s) 2055
SapI GCTCTTC 1 cut(s) 2043
SaqAI TTAA 8 cut(s) 213, 372, 465, 666, 1401, 2568, 2589, 2786
SatI GCNGC 6 cut(s) 622, 1290, 1326, 1517, 2168, 2796
Sau96I GGNCC 2 cut(s) 549, 1177
SchI GAGTC 6 cut(s) 40, 249, 624, 1229, 2023, 2501
ScrFI CCNGG 6 cut(s) 123, 224, 238, 732, 1256, 2057
SduI GDGCHC 4 cut(s) 1138, 1410, 2008, 2055
SexAI ACCWGGT 1 cut(s) 730
SfcI CTRYAG 4 cut(s) 259, 786, 863, 1326
Sfr274I CTCGAG 1 cut(s) 642
SinI GGWCC 2 cut(s) 549, 1177
SlaI CTCGAG 1 cut(s) 642
SmiMI CAYNNNNRTG 1 cut(s) 1413
SmlI CTYRAG 8 cut(s) 371, 496, 642, 1499, 1601, 2033, 2588, 2642
SmoI CTYRAG 8 cut(s) 371, 496, 642, 1499, 1601, 2033, 2588, 2642
SsiI CCGC 3 cut(s) 574, 926, 1864
SspI AATATT 1 cut(s) 1266
SspMI CTAG 7 cut(s) 1574, 1635, 1670, 1766, 1872, 2108, 3133
SstI GAGCTC 1 cut(s) 2055
StyD4I CCNGG 6 cut(s) 121, 222, 236, 730, 1254, 2055
StyI CCWWGG 1 cut(s) 1419
TaaI ACNGT 7 cut(s) 145, 211, 774, 1807, 2261, 2897, 3103
TaiI ACGT 1 cut(s) 2533
TatI WGTACW 2 cut(s) 652, 2384
TfiI GAWTC 6 cut(s) 118, 403, 791, 1237, 1310, 2347
Tru1I TTAA 8 cut(s) 213, 372, 465, 666, 1401, 2568, 2589, 2786
Tru9I TTAA 8 cut(s) 213, 372, 465, 666, 1401, 2568, 2589, 2786
TscAI CASTG 4 cut(s) 779, 1593, 1636, 2519
TseFI GTSAC 2 cut(s) 674, 2015
TseI GCWGC 6 cut(s) 621, 1289, 1325, 1516, 2167, 2795
Tsp45I GTSAC 2 cut(s) 674, 2015
TspRI CASTG 4 cut(s) 779, 1593, 1636, 2519
Vha464I CTTAAG 2 cut(s) 371, 2588
VneI GTGCAC 1 cut(s) 2004
VpaK11BI GGWCC 2 cut(s) 549, 1177
XapI RAATTY 6 cut(s) 215, 376, 1913, 2289, 3059, 3118
XbaI TCTAGA 1 cut(s) 1669
XceI RCATGY 3 cut(s) 398, 934, 1815
XcmI CCANNNNNNNNNTGG 1 cut(s) 1118
XhoI CTCGAG 1 cut(s) 642
XspI CTAG 7 cut(s) 1574, 1635, 1670, 1766, 1872, 2108, 3133
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.