pycom17g01510

PGAP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0005447
Physical Location & Seq
Forward (+)
1012582 .. 1017440
4859 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2301 bp
ATGCAACTGCAAGGATTTAAAGCCAAATCCAGAGTAGCACTGCTCGTCGCCTTCGTCTTGTGTGTTGGTTTCGCGGGTTTATATGATTTGTTGAAGCCCGTCTCCAATGGCTGCATCATGACGTACATGTATCCCACCTATATTCCCATCCCTACTACGACTCCCGTTTCTCCTGCGAAATACAGCTTGTATTTGTACCACGAAGGATGGAAGCAGATCGATTTTGAGGAGCATCTCAAGAAGCTGAGCGGCATTCCCGTTCTTTTTGTTCCGGGAAATGGTGGTAGCTACAAGCAGGTTCGATCACTTGCAGCAGAATCTGATAGGGCATACCAAGCGGGGCCCCTAGAGCGTACGTACTATCAGGAAGCTTACTTAACTCCTGAAGAGGGAGGGGAGGAGATTGATGTGACTTCCTTTAAGTTGCCCAACCAGTATGATTCCAGGTTGGACTGGTTCACGGTGGATCTTGAAGGGGAACATTCTGCTATGGATAGTGCTATTCTTGAAGAACATGCTGAATTTGTTGTCAACTCCATTCACAGGATTTTGGATCAATACAAGGAATCTTATGAGGCTAGACAAAGAGAAGGTGCTGCAACCTCTGGGAGTTCCCCAAAAAGTGTGATATTGGTTGGTCACTCTATGGGTGGTTTTGTTGCTAGAGCTGCAGTTATCCATCACCGTTTGAGGAAATCAGCAGTTGAAACAATTGTTACTCTCTCCAGCCCCCACCAATATCCTCCTGTGGCATTGCAGCCTTCCTTGGGCCACTACTTTGAACGAGTTAATGATGAATGGAGAAAGGGATATGAGGTCCATACAACTAGAGCAGGACATCACGTGTCTGGTCCTGTTCTCTCCCATGTTGTTGTTATATCCATTTCTGGTAGTTATAATGATTATCAGGTAAGGTCCAAGTCAGAATCACTTGATGGCATTGTGCCTCCCACTCACGGTTTTGTGATCAGTAGTACGGGCATGAGAAATGTGTGGTTGTCAATGGAACATCAAGCTATTTTATGGTGTAATCAATTAGTTATCCAAGTAGCGCACACTCTCCTTAGTTTGGTAGACTCCGGTACAGGCCAGCCATTTTCTAACACTCGAATAAGAATGGCGATATTCTCAAAAATGCTTCGTAGTGGGATACCACAGAGCTTCAATTGGAGGATGCAATCTTACTTCTCTCAGAAGTCGATACATGTTCCTACTAGAGACATAAAAGATAAAACTGAATCACTGTACACTTCCGCTGCATGTCCTAGCAATGTTCATTGGAGTGATGATGGCCTTGAGAGGGACCTATACATTCAGACGACTACCGTCACTGTTTTAGCTATGGATGGTCGAAGACGGTGGTTAGACATACAAAAACTGGGGTCAAATGGAAGAAGCCACTTCATGTTCGTGACAAACCTCGCTCCATGTTCTGGGGTTAGACTTCATCTGTGGCCGGAAAAAAGAAACTCAACTTCAAAATTGCCTATTTGTATAAGAATCCTCGAAGTGACATCGAGGATGGTGCGCATTCCATCAGGACCAGCACCAACACAGATTGAACCTGGAGGCCAGACAGAGCAAGCTCCTCCTTCTGCTATATTTCACTTGGGACCTGAGGACATGCGTGGTTTCAGATTCCTGACCGTATCAGTTGCACCTCGTCCGACTATTTCAGGCAGACCTCCACCAGCAGTTTCCATGGCTGTTGGGCAGTTTTTTAATCCAGAGGAAGGGGAACGAGAGTTCTCCCCTTGGTCTATGCCGCTATCTAGTTATTCTTATAAGGACATGTCTTTGAAGGAGAATCATCCTCTCGCTCTGAATCTATCATTTACCACGAGCTTGGGTCTTCTGCCTGTCATATTCTCTTTGAAGACTGCAGGTTGTGGGATAAAGAATTCTGGACTTCCAGATGAACAGGCTGGAGATGAAGATAATAGCAAGTTGTGCAAGCTTCGCTGTTTCCCCCCTATAGCATTTGCTTGGGATGACACGTCAGGTCTCCACATATTCCCGAATATGTACAATGAGAAAATTGTTGTTGATTCTTCACCAGCACTATGGGGTTCATCTAAAAGTTCTGAGAAAACCTCTGTTATGTTGCTGGTAGATCCACATTGTTCATATAGAAGTTCCATGACTCTTTCTGTCACTGCAGTTGCGAGTAGGTTCTTGCTTTTATATAATTCACAGATTGCTGGTTTCTCCCTTGTTGTTATCTTTTTTGCATTAATGCAGCAAATATGTACGTGGGATCGATCAGCACATACCTTCGATTCTTACGGCTGTGGAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
PGAP1

Protein Analysis

767

Amino Acids

85.27

Weight (kDa)

7.13

Isoelectric Point (pI)

43.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PGAP1 PF07819 82 - 356 7.2e-82 PGAP1-like alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 897, 1785
Acc16I TGCGCA 1 cut(s) 1529
Acc36I ACCTGC 2 cut(s) 286, 1874
AccB7I CCANNNNNTGG 1 cut(s) 1433
AccBSI CCGCTC 1 cut(s) 249
AccI GTMKAC 1 cut(s) 1074
AccII CGCG 1 cut(s) 74
AciI CCGC 5 cut(s) 74, 249, 338, 1254, 1766
AclWI GGATC 4 cut(s) 474, 561, 2108, 2265
AcoI YGGCCR 1 cut(s) 1454
AcsI RAATTY 3 cut(s) 521, 1900, 2295
AcuI CTGAAG 1 cut(s) 405
AcvI CACGTG 1 cut(s) 844
AfaI GTAC 9 cut(s) 125, 197, 355, 359, 976, 1084, 1247, 2027, 2251
AfiI CCNNNNNNNGG 9 cut(s) 278, 389, 543, 767, 956, 1069, 1300, 1433, 1733
AflIII ACRYGT 5 cut(s) 126, 843, 1204, 1791, 1995
AjiI CACGTC 1 cut(s) 1998
AjnI CCWGG 2 cut(s) 443, 1564
AloI GAACNNNNNNTCC 2 cut(s) 1730, 1762
Alw26I GTCTC 3 cut(s) 106, 1212, 2009
AlwI GGATC 4 cut(s) 474, 561, 2108, 2265
AlwNI CAGNNNCTG 1 cut(s) 320
AoxI GGCC 6 cut(s) 341, 769, 1087, 1291, 1454, 1570
ApaI GGGCCC 1 cut(s) 345
ApeKI GCWGC 7 cut(s) 111, 311, 596, 668, 757, 1256, 2239
ApoI RAATTY 3 cut(s) 521, 1900, 2295
AseI ATTAAT 1 cut(s) 2234
AspLEI GCGC 2 cut(s) 1054, 1530
AspS9I GGNCC 9 cut(s) 341, 342, 769, 817, 851, 915, 1303, 1541, 1613
AsuC2I CCSGG 1 cut(s) 273
AsuHPI GGTGA 2 cut(s) 674, 2046
AvaII GGWCC 6 cut(s) 817, 851, 915, 1303, 1541, 1613
AxyI CCTNAGG 1 cut(s) 1617
BaeGI GKGCMC 1 cut(s) 345
BanII GRGCYC 1 cut(s) 345
BauI CACGAG 1 cut(s) 1840
BbrPI CACGTG 1 cut(s) 844
BbsI GAAGAC 3 cut(s) 1360, 1844, 1883
BbvI GCAGC 7 cut(s) 98, 323, 583, 655, 769, 1243, 2251
BccI CCATC 8 cut(s) 155, 201, 687, 929, 1283, 1340, 1516, 1543
BcgI CGANNNNNNTGC 2 cut(s) 1507, 1541
BciT130I CCWGG 2 cut(s) 445, 1566
BciVI GTATCC 2 cut(s) 141, 1143
BclI TGATCA 1 cut(s) 966
BcnI CCSGG 1 cut(s) 273
BcoDI GTCTC 3 cut(s) 106, 1212, 2009
BfaI CTAG 7 cut(s) 347, 579, 663, 828, 1215, 1266, 1773
BfmI CTRYAG 4 cut(s) 669, 1881, 1974, 2157
BfuAI ACCTGC 2 cut(s) 286, 1874
BfuI GTATCC 2 cut(s) 141, 1143
BisI GCNGC 9 cut(s) 112, 250, 312, 597, 669, 758, 1257, 1766, 2240
BlpI GCTNAGC 1 cut(s) 245
BlsI GCNGC 9 cut(s) 113, 251, 313, 598, 670, 759, 1258, 1767, 2241
Bme1390I CCNGG 3 cut(s) 273, 445, 1566
Bme18I GGWCC 6 cut(s) 817, 851, 915, 1303, 1541, 1613
BmgBI CACGTC 1 cut(s) 1998
BmgT120I GGNCC 9 cut(s) 341, 342, 769, 817, 851, 915, 1303, 1541, 1613
BmiI GGNNCC 5 cut(s) 342, 343, 344, 1304, 1614
BmrFI CCNGG 3 cut(s) 273, 445, 1566
BmrI ACTGGG 1 cut(s) 1388
BmsI GCATC 3 cut(s) 123, 241, 1164
BmuI ACTGGG 1 cut(s) 1388
BoxI GACNNNNGTC 1 cut(s) 1325
BpiI GAAGAC 3 cut(s) 1360, 1844, 1883
BplI GAGNNNNNCTC 2 cut(s) 2078, 2110
BpmI CTGGAG 3 cut(s) 709, 1587, 1947
Bpu1102I GCTNAGC 1 cut(s) 245
BpuEI CTTGAG 2 cut(s) 221, 1316
BpuMI CCSGG 1 cut(s) 273
Bsa29I ATCGAT 2 cut(s) 219, 2260
BsaAI YACGTR 3 cut(s) 357, 844, 2253
BsaI GGTCTC 1 cut(s) 2009
BsaJI CCNNGG 3 cut(s) 765, 1701, 1754
BsaWI WCCGGW 1 cut(s) 1079
BsaXI ACNNNNNCTCC 2 cut(s) 145, 175
Bsc4I CCNNNNNNNGG 9 cut(s) 278, 389, 543, 767, 956, 1069, 1300, 1433, 1733
Bse1I ACTGG 3 cut(s) 433, 458, 1383
Bse21I CCTNAGG 1 cut(s) 1617
Bse3DI GCAATG 2 cut(s) 752, 1276
BseBI CCWGG 2 cut(s) 445, 1566
BseCI ATCGAT 2 cut(s) 219, 2260
BseDI CCNNGG 3 cut(s) 765, 1701, 1754
BseGI GGATG 7 cut(s) 147, 212, 1179, 1351, 1527, 1810, 1996
BseLI CCNNNNNNNGG 9 cut(s) 278, 389, 543, 767, 956, 1069, 1300, 1433, 1733
BseMI GCAATG 2 cut(s) 752, 1276
BseMII CTCAG 4 cut(s) 236, 1205, 1608, 2076
BseNI ACTGG 3 cut(s) 433, 458, 1383
BseRI GAGGAG 3 cut(s) 242, 413, 1578
BseSI GKGCMC 1 cut(s) 345
BseXI GCAGC 7 cut(s) 98, 323, 583, 655, 769, 1243, 2251
Bsh1236I CGCG 1 cut(s) 74
BshFI GGCC 6 cut(s) 343, 771, 1089, 1293, 1456, 1572
BshVI ATCGAT 2 cut(s) 219, 2260
BsiSI CCGG 3 cut(s) 272, 1080, 1457
BsiWI CGTACG 1 cut(s) 353
BslFI GGGAC 2 cut(s) 1316, 1626
BslI CCNNNNNNNGG 9 cut(s) 278, 389, 543, 767, 956, 1069, 1300, 1433, 1733
BsmAI GTCTC 3 cut(s) 106, 1212, 2009
BsmBI CGTCTC 1 cut(s) 106
BsmFI GGGAC 2 cut(s) 1316, 1626
BsmI GAATGC 2 cut(s) 252, 1530
BsnI GGCC 6 cut(s) 343, 771, 1089, 1293, 1456, 1572
Bso31I GGTCTC 1 cut(s) 2009
Bsp120I GGGCCC 1 cut(s) 341
Bsp1286I GDGCHC 1 cut(s) 345
Bsp1407I TGTACA 2 cut(s) 1245, 2025
Bsp143I GATC 8 cut(s) 216, 302, 466, 553, 966, 2113, 2257, 2261
Bsp1720I GCTNAGC 1 cut(s) 245
Bsp19I CCATGG 1 cut(s) 1701
BspACI CCGC 5 cut(s) 74, 249, 338, 1254, 1766
BspANI GGCC 6 cut(s) 343, 771, 1089, 1293, 1456, 1572
BspCNI CTCAG 4 cut(s) 237, 1204, 1609, 2077
BspDI ATCGAT 2 cut(s) 219, 2260
BspFNI CGCG 1 cut(s) 74
BspHI TCATGA 1 cut(s) 117
BspLI GGNNCC 5 cut(s) 342, 343, 344, 1304, 1614
BspMAI CTGCAG 3 cut(s) 673, 1885, 2161
BspMI ACCTGC 2 cut(s) 286, 1874
BspPI GGATC 4 cut(s) 474, 561, 2108, 2265
BspTNI GGTCTC 1 cut(s) 2009
BsrBI CCGCTC 1 cut(s) 249
BsrDI GCAATG 2 cut(s) 752, 1276
BsrGI TGTACA 2 cut(s) 1245, 2025
BsrI ACTGG 3 cut(s) 433, 458, 1383
BssECI CCNNGG 3 cut(s) 765, 1701, 1754
BssMI GATC 8 cut(s) 216, 302, 466, 553, 966, 2113, 2257, 2261
BssSI CACGAG 1 cut(s) 1840
BssT1I CCWWGG 3 cut(s) 765, 1701, 1754
Bst2BI CACGAG 1 cut(s) 1840
Bst2UI CCWGG 2 cut(s) 445, 1566
Bst4CI ACNGT 8 cut(s) 463, 686, 959, 1245, 1327, 1333, 1359, 1648
Bst6I CTCTTC 1 cut(s) 381
BstAPI GCANNNNNTGC 1 cut(s) 1950
BstAUI TGTACA 2 cut(s) 1245, 2025
BstBAI YACGTR 3 cut(s) 357, 844, 2253
BstC8I GCNNGC 3 cut(s) 1091, 1584, 1955
BstDEI CTNAG 5 cut(s) 245, 1064, 1191, 1617, 2085
BstDSI CCRYGG 1 cut(s) 1701
BstF5I GGATG 7 cut(s) 147, 212, 1179, 1351, 1527, 1810, 1996
BstFNI CGCG 1 cut(s) 74
BstHHI GCGC 2 cut(s) 1054, 1530
BstKTI GATC 8 cut(s) 219, 305, 469, 556, 969, 2116, 2260, 2264
BstMAI GTCTC 3 cut(s) 106, 1212, 2009
BstMBI GATC 8 cut(s) 216, 302, 466, 553, 966, 2113, 2257, 2261
BstMWI GCNNNNNNNGC 5 cut(s) 335, 349, 668, 1950, 1959
BstNI CCWGG 2 cut(s) 445, 1566
BstNSI RCATGY 6 cut(s) 130, 518, 1208, 1263, 1627, 1795
BstPAI GACNNNNGTC 1 cut(s) 1325
BstSCI CCNGG 3 cut(s) 271, 443, 1564
BstSFI CTRYAG 4 cut(s) 669, 1881, 1974, 2157
BstSLI GKGCMC 1 cut(s) 345
BstSNI TACGTA 1 cut(s) 357
BstUI CGCG 1 cut(s) 74
BstV1I GCAGC 7 cut(s) 98, 323, 583, 655, 769, 1243, 2251
BstV2I GAAGAC 3 cut(s) 1360, 1844, 1883
BstX2I RGATCY 2 cut(s) 466, 2113
BstXI CCANNNNNNTGG 2 cut(s) 1846, 2064
BstYI RGATCY 2 cut(s) 466, 2113
Bsu15I ATCGAT 2 cut(s) 219, 2260
Bsu36I CCTNAGG 1 cut(s) 1617
BsuI GTATCC 2 cut(s) 141, 1143
BsuRI GGCC 6 cut(s) 343, 771, 1089, 1293, 1456, 1572
BsuTUI ATCGAT 2 cut(s) 219, 2260
BtgI CCRYGG 1 cut(s) 1701
BtrI CACGTC 1 cut(s) 1998
BtsCI GGATG 7 cut(s) 147, 212, 1179, 1351, 1527, 1810, 1996
BtsI GCAGTG 2 cut(s) 38, 2154
BtsIMutI CAGTG 4 cut(s) 38, 1241, 1329, 2154
BveI ACCTGC 2 cut(s) 286, 1874
Cac8I GCNNGC 3 cut(s) 1091, 1584, 1955
CaiI CAGNNNCTG 1 cut(s) 320
CciI TCATGA 1 cut(s) 117
CfoI GCGC 2 cut(s) 1054, 1530
Cfr13I GGNCC 9 cut(s) 341, 342, 769, 817, 851, 915, 1303, 1541, 1613
ClaI ATCGAT 2 cut(s) 219, 2260
Csp6I GTAC 9 cut(s) 124, 196, 354, 358, 975, 1083, 1246, 2026, 2250
CviQI GTAC 9 cut(s) 124, 196, 354, 358, 975, 1083, 1246, 2026, 2250
DdeI CTNAG 5 cut(s) 245, 1064, 1191, 1617, 2085
DpnI GATC 8 cut(s) 218, 304, 468, 555, 968, 2115, 2259, 2263
DpnII GATC 8 cut(s) 216, 302, 466, 553, 966, 2113, 2257, 2261
DraI TTTAAA 1 cut(s) 19
EaeI YGGCCR 1 cut(s) 1454
Eam1104I CTCTTC 1 cut(s) 381
EarI CTCTTC 1 cut(s) 381
Eco105I TACGTA 1 cut(s) 357
Eco130I CCWWGG 3 cut(s) 765, 1701, 1754
Eco24I GRGCYC 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 2009
Eco47I GGWCC 6 cut(s) 817, 851, 915, 1303, 1541, 1613
Eco57I CTGAAG 1 cut(s) 405
Eco72I CACGTG 1 cut(s) 844
Eco81I CCTNAGG 1 cut(s) 1617
EcoO109I RGGNCCY 4 cut(s) 341, 342, 1303, 1613
EcoRI GAATTC 1 cut(s) 1900
EcoRII CCWGG 2 cut(s) 443, 1564
EcoT14I CCWWGG 3 cut(s) 765, 1701, 1754
EcoT38I GRGCYC 1 cut(s) 345
ErhI CCWWGG 3 cut(s) 765, 1701, 1754
Esp3I CGTCTC 1 cut(s) 106
FaqI GGGAC 2 cut(s) 1316, 1626
FauI CCCGC 2 cut(s) 67, 331
FbaI TGATCA 1 cut(s) 966
FblI GTMKAC 1 cut(s) 1074
Fnu4HI GCNGC 9 cut(s) 112, 250, 312, 597, 669, 758, 1257, 1766, 2240
FokI GGATG 7 cut(s) 134, 219, 1186, 1358, 1534, 1797, 2003
FriOI GRGCYC 1 cut(s) 345
Fsp4HI GCNGC 9 cut(s) 112, 250, 312, 597, 669, 758, 1257, 1766, 2240
FspAI RTGCGCAY 1 cut(s) 1529
FspBI CTAG 7 cut(s) 347, 579, 663, 828, 1215, 1266, 1773
FspI TGCGCA 1 cut(s) 1529
GlaI GCGC 2 cut(s) 1053, 1529
GluI GCNGC 9 cut(s) 112, 250, 312, 597, 669, 758, 1257, 1766, 2240
GsuI CTGGAG 3 cut(s) 709, 1587, 1947
HaeIII GGCC 6 cut(s) 343, 771, 1089, 1293, 1456, 1572
HapII CCGG 3 cut(s) 272, 1080, 1457
HhaI GCGC 2 cut(s) 1054, 1530
Hin6I GCGC 2 cut(s) 1052, 1528
HinP1I GCGC 2 cut(s) 1052, 1528
HincII GTYRAC 1 cut(s) 532
HindII GTYRAC 1 cut(s) 532
HindIII AAGCTT 2 cut(s) 369, 1955
HpaII CCGG 3 cut(s) 272, 1080, 1457
HphI GGTGA 2 cut(s) 674, 2046
Hpy166II GTNNAC 4 cut(s) 459, 532, 1075, 1248
Hpy188I TCNGA 8 cut(s) 322, 925, 1194, 1317, 1637, 1668, 1824, 2086
Hpy8I GTNNAC 4 cut(s) 459, 532, 1075, 1248
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 9 cut(s) 61, 197, 467, 584, 771, 1602, 1727, 1795, 2284
HpyCH4III ACNGT 8 cut(s) 463, 686, 959, 1245, 1327, 1333, 1359, 1648
HpyCH4IV ACGT 5 cut(s) 122, 356, 843, 1997, 2252
HpyF10VI GCNNNNNNNGC 5 cut(s) 335, 349, 668, 1950, 1959
HpyF3I CTNAG 5 cut(s) 245, 1064, 1191, 1617, 2085
HpySE526I ACGT 5 cut(s) 122, 356, 843, 1997, 2252
HspAI GCGC 2 cut(s) 1052, 1528
Ksp22I TGATCA 1 cut(s) 966
Kzo9I GATC 8 cut(s) 216, 302, 466, 553, 966, 2113, 2257, 2261
LmnI GCTCC 3 cut(s) 229, 1429, 1591
Lsp1109I GCAGC 7 cut(s) 98, 323, 583, 655, 769, 1243, 2251
LweI GCATC 3 cut(s) 123, 241, 1164
MaeI CTAG 7 cut(s) 347, 579, 663, 828, 1215, 1266, 1773
MaeII ACGT 5 cut(s) 122, 356, 843, 1997, 2252
MaeIII GTNAC 7 cut(s) 409, 638, 715, 1327, 1411, 1510, 2152
MalI GATC 8 cut(s) 218, 304, 468, 555, 968, 2115, 2259, 2263
MbiI CCGCTC 1 cut(s) 249
MboI GATC 8 cut(s) 216, 302, 466, 553, 966, 2113, 2257, 2261
MboII GAAGA 8 cut(s) 398, 521, 1365, 1404, 1844, 1888, 1946, 2043
MfeI CAATTG 2 cut(s) 711, 1165
MflI RGATCY 2 cut(s) 466, 2113
MhlI GDGCHC 1 cut(s) 345
MluCI AATT 9 cut(s) 521, 711, 1034, 1165, 1481, 1900, 2037, 2188, 2295
MlyI GAGTC 3 cut(s) 154, 1070, 2137
MmeI TCCRAC 2 cut(s) 429, 1691
MseI TTAA 7 cut(s) 18, 377, 420, 789, 1722, 2234, 2299
MslI CAYNNNNRTG 2 cut(s) 1281, 1409
MspA1I CMGCKG 1 cut(s) 1256
MspI CCGG 3 cut(s) 272, 1080, 1457
MspR9I CCNGG 3 cut(s) 273, 445, 1566
MunI CAATTG 2 cut(s) 711, 1165
Mva1269I GAATGC 2 cut(s) 252, 1530
MvaI CCWGG 2 cut(s) 445, 1566
MvnI CGCG 1 cut(s) 74
MwoI GCNNNNNNNGC 5 cut(s) 335, 349, 668, 1950, 1959
NciI CCSGG 1 cut(s) 273
NcoI CCATGG 1 cut(s) 1701
NdeII GATC 8 cut(s) 216, 302, 466, 553, 966, 2113, 2257, 2261
NlaIV GGNNCC 5 cut(s) 342, 343, 344, 1304, 1614
NmuCI GTSAC 6 cut(s) 409, 638, 1327, 1411, 1510, 2152
NsbI TGCGCA 1 cut(s) 1529
NspI RCATGY 6 cut(s) 130, 518, 1208, 1263, 1627, 1795
PagI TCATGA 1 cut(s) 117
PciI ACATGT 3 cut(s) 126, 1204, 1791
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PctI GAATGC 2 cut(s) 252, 1530
Pfl23II CGTACG 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 1433
PfoI TCCNGGA 1 cut(s) 271
PkrI GCNGC 9 cut(s) 113, 251, 313, 598, 670, 759, 1258, 1767, 2241
PleI GAGTC 3 cut(s) 154, 1070, 2137
PmaCI CACGTG 1 cut(s) 844
PmlI CACGTG 1 cut(s) 844
PpsI GAGTC 3 cut(s) 154, 1070, 2137
Ppu21I YACGTR 3 cut(s) 357, 844, 2253
PpuMI RGGWCCY 2 cut(s) 1303, 1613
PscI ACATGT 3 cut(s) 126, 1204, 1791
PshAI GACNNNNGTC 1 cut(s) 1325
PshBI ATTAAT 1 cut(s) 2234
PsiI TTATAA 2 cut(s) 897, 1785
Psp5II RGGWCCY 2 cut(s) 1303, 1613
Psp6I CCWGG 2 cut(s) 443, 1564
PspCI CACGTG 1 cut(s) 844
PspGI CCWGG 2 cut(s) 443, 1564
PspLI CGTACG 1 cut(s) 353
PspN4I GGNNCC 5 cut(s) 342, 343, 344, 1304, 1614
PspOMI GGGCCC 1 cut(s) 341
PspPI GGNCC 9 cut(s) 341, 342, 769, 817, 851, 915, 1303, 1541, 1613
PspPPI RGGWCCY 2 cut(s) 1303, 1613
PstI CTGCAG 3 cut(s) 673, 1885, 2161
PstNI CAGNNNCTG 1 cut(s) 320
PsuI RGATCY 2 cut(s) 466, 2113
RsaI GTAC 9 cut(s) 125, 197, 355, 359, 976, 1084, 1247, 2027, 2251
RsaNI GTAC 9 cut(s) 124, 196, 354, 358, 975, 1083, 1246, 2026, 2250
RseI CAYNNNNRTG 2 cut(s) 1281, 1409
SaqAI TTAA 7 cut(s) 18, 377, 420, 789, 1722, 2234, 2299
SatI GCNGC 9 cut(s) 112, 250, 312, 597, 669, 758, 1257, 1766, 2240
Sau3AI GATC 8 cut(s) 216, 302, 466, 553, 966, 2113, 2257, 2261
Sau96I GGNCC 9 cut(s) 341, 342, 769, 817, 851, 915, 1303, 1541, 1613
SchI GAGTC 3 cut(s) 154, 1070, 2137
ScrFI CCNGG 3 cut(s) 273, 445, 1566
SduI GDGCHC 1 cut(s) 345
SfaNI GCATC 3 cut(s) 123, 241, 1164
SfcI CTRYAG 4 cut(s) 669, 1881, 1974, 2157
SinI GGWCC 6 cut(s) 817, 851, 915, 1303, 1541, 1613
SmiMI CAYNNNNRTG 2 cut(s) 1281, 1409
SmlI CTYRAG 2 cut(s) 236, 1295
SmoI CTYRAG 2 cut(s) 236, 1295
SnaBI TACGTA 1 cut(s) 357
Sse9I AATT 9 cut(s) 521, 711, 1034, 1165, 1481, 1900, 2037, 2188, 2295
SsiI CCGC 5 cut(s) 74, 249, 338, 1254, 1766
SspMI CTAG 7 cut(s) 347, 579, 663, 828, 1215, 1266, 1773
StyD4I CCNGG 3 cut(s) 271, 443, 1564
StyI CCWWGG 3 cut(s) 765, 1701, 1754
TaaI ACNGT 8 cut(s) 463, 686, 959, 1245, 1327, 1333, 1359, 1648
TaiI ACGT 5 cut(s) 125, 359, 846, 2000, 2255
TaqI TCGA 9 cut(s) 219, 301, 1108, 1199, 1351, 1506, 1517, 2260, 2277
TasI AATT 9 cut(s) 521, 711, 1034, 1165, 1481, 1900, 2037, 2188, 2295
TatI WGTACW 2 cut(s) 1245, 2025
TauI GCSGC 2 cut(s) 252, 1768
Tru1I TTAA 7 cut(s) 18, 377, 420, 789, 1722, 2234, 2299
Tru9I TTAA 7 cut(s) 18, 377, 420, 789, 1722, 2234, 2299
TscAI CASTG 4 cut(s) 45, 1248, 1336, 2161
TseFI GTSAC 6 cut(s) 409, 638, 1327, 1411, 1510, 2152
TseI GCWGC 7 cut(s) 111, 311, 596, 668, 757, 1256, 2239
Tsp45I GTSAC 6 cut(s) 409, 638, 1327, 1411, 1510, 2152
TspDTI ATGAA 8 cut(s) 810, 1265, 1393, 1436, 1932, 1947, 2061, 2115
TspRI CASTG 4 cut(s) 45, 1248, 1336, 2161
Van91I CCANNNNNTGG 1 cut(s) 1433
VpaK11BI GGWCC 6 cut(s) 817, 851, 915, 1303, 1541, 1613
VspI ATTAAT 1 cut(s) 2234
XapI RAATTY 3 cut(s) 521, 1900, 2295
XceI RCATGY 6 cut(s) 130, 518, 1208, 1263, 1627, 1795
XmiI GTMKAC 1 cut(s) 1074
XspI CTAG 7 cut(s) 347, 579, 663, 828, 1215, 1266, 1773
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.