pycom17g01860

Methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0010488
Physical Location & Seq
Forward (+)
1183767 .. 1186303
2537 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1509 bp
ATGAAGCACAAGAGCGAGCTCAGCTCTTTCCCCACGGCGAACAAGCTGCTGAAGTTCGCTCTGATCGGTCTCATTGGGTGCCTGGGCTTGCTCTGTTTGTACTACGGGTGGTCTTTCGGGCCGGGCTCCCGGAGATCCGACGAGGAGGCTTCTGGGTCCGATGGAGTCGACCCGCTTTTCGGCGGGTTCGTTCTCCATCACGATTTGGATGATTTGCATGACGACCAGGAGCACAACTCTGAGGTCCCCAAAAGCTTGCCTATATGCGATTTACGGTTTTCGGAGTTGATACCTTGTCTGGATAGAAACCTTATTTACCAACTGAGATTAAAGCCGAATTTGACGTTAATGGAGCATTACGAACGGCACTGCCCGCCTCCAGAGCGGCGTTATAATTGCTTGATACCGCCCCCACTTGGTTATAAGATACCTATAAGATGGCCGGCAAGTAGAGATGAAGTGTGGAAGGCCAACATTCCCCACACCCATCTTGCTCAAGAGAAGTCAGATCAGAATTGGATGGTTGTGAATGGGGACAAGATCAATTTTCCGGGTGGTGGGACACATTTCCATGATGGAGCTGATAAGTACATTGTTGCTCTTTCCAGGATGCTTAAATTTCCTGGTGATAAATTCAATAATGGTGGAAATATCCGTAATGTTCTCGATGTGGGTTGTGGAGTTGCGAGTTTTGGGGCATATCTTCTTTCTCATGACGTCATAGCTCTGTCCCTTGCTCCAAATGATGTGCATGAGAATCAAATACAATTTGCACTAGAGAGGGGCATTCCCTCTACTCTTGGTATCTTGGGTACAAAAAGACTTACTTATCCAAGCAGATCATTTGAGCTGGCTCATTGCTCTCGGTGTCGAATAGATTGGCTTCAAAGAGATGGAATCCTGTTATTAGAGCTGGACAGATTACTAAGACCTGGAGGGTATTTTGTGTACACTTCTCCTGAAGCATATGCAAATGATCCGGAAAATCGGAGGATCTGGAATGCTATGTATGATCTTCTGAAAAGAATGTGCTGGAGAGTTGTAACAAAGAAAGAGCAGAGTGTTGTATGGGCCAAGCCACTGACTAATAGTTGTTACTTAAAGAGAGATTCTAGGACCCGGCCCCCTCTCTGCGATTCCAAGGATGACCCTGATGCAAGTTGGAATGTGAGCATGAAGGCGTGCATTTCTCGATACTCTTCAAAGATGCACAAGGAAAGAGGAAGTGGGCTAGCTCCTTGGCCACAGAGGCTTACCGCTGCACCGCCTCGCCTGGAAGAAATTGGTGTCAGTCCTGAAGAATTCCAAGAGGACACTAGCATATGGCACTTCAGAGTGATTGAGTACTGGAAGCAGATGAAGTCTGTCATACAGAAAAATTCAATTAGAAATGTCATGGATATGAACTCATACCTTGGTGGATTTGCTGCTGCCCTGAATGAAAAAGACGTCTGGGTGATGAATGTTGCTCCTGTCCATGTATCTGCCAGACTCAAGATCGATCTATGA

Protein Analysis

503

Amino Acids

57.16

Weight (kDa)

8.14

Isoelectric Point (pI)

50.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_29 PF03141 95 - 500 3.7e-176 Putative S-adenosyl-L-methionine-dependent methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 393, 423
AatII GACGTC 2 cut(s) 720, 1452
AccB1I GGYRCC 1 cut(s) 78
AccBSI CCGCTC 1 cut(s) 385
AccI GTMKAC 1 cut(s) 168
AccIII TCCGGA 1 cut(s) 979
AciI CCGC 7 cut(s) 173, 183, 374, 385, 407, 1257, 1265
AclWI GGATC 3 cut(s) 129, 971, 1001
AcoI YGGCCR 2 cut(s) 440, 1241
AcsI RAATTY 5 cut(s) 337, 617, 632, 1301, 1378
AcuI CTGAAG 4 cut(s) 71, 981, 1315, 1317
AcyI GRCGYC 2 cut(s) 717, 1449
AfaI GTAC 5 cut(s) 101, 590, 814, 950, 1346
AfiI CCNNNNNNNGG 3 cut(s) 179, 416, 557
AgsI TTSAA 4 cut(s) 637, 887, 1203, 1383
AjnI CCWGG 6 cut(s) 81, 225, 605, 622, 931, 1272
AluBI AGCT 9 cut(s) 19, 24, 46, 255, 581, 725, 850, 913, 1235
AluI AGCT 9 cut(s) 19, 24, 46, 255, 581, 725, 850, 913, 1235
Alw21I GWGCWC 2 cut(s) 21, 234
Alw26I GTCTC 1 cut(s) 74
AlwI GGATC 3 cut(s) 129, 971, 1001
Aor13HI TCCGGA 1 cut(s) 979
AoxI GGCC 6 cut(s) 119, 440, 468, 1071, 1121, 1241
ApeKI GCWGC 4 cut(s) 46, 1259, 1427, 1430
ApoI RAATTY 5 cut(s) 337, 617, 632, 1301, 1378
ArsI GACNNNNNNTTYG 2 cut(s) 161, 193
AspS9I GGNCC 6 cut(s) 119, 156, 244, 1071, 1116, 1122
AsuC2I CCSGG 4 cut(s) 123, 130, 552, 1120
AsuHPI GGTGA 2 cut(s) 638, 1468
AsuNHI GCTAGC 1 cut(s) 1231
AvaII GGWCC 3 cut(s) 156, 244, 1116
BalI TGGCCA 1 cut(s) 1243
BanI GGYRCC 1 cut(s) 78
BanII GRGCYC 2 cut(s) 21, 128
Bbv12I GWGCWC 2 cut(s) 21, 234
BbvI GCAGC 4 cut(s) 33, 1246, 1414, 1417
BccI CCATC 7 cut(s) 155, 204, 432, 495, 514, 569, 887
BceAI ACGGC 2 cut(s) 51, 380
BcgI CGANNNNNNTGC 2 cut(s) 28, 62
BciT130I CCWGG 6 cut(s) 83, 227, 607, 624, 933, 1274
BcnI CCSGG 4 cut(s) 123, 130, 552, 1120
BcoDI GTCTC 1 cut(s) 74
BfaI CTAG 4 cut(s) 776, 1113, 1232, 1317
BglI GCCNNNNNGGC 1 cut(s) 1249
BisI GCNGC 5 cut(s) 47, 386, 1260, 1428, 1431
BlpI GCTNAGC 1 cut(s) 20
BlsI GCNGC 5 cut(s) 48, 387, 1261, 1429, 1432
BmcAI AGTACT 1 cut(s) 1346
Bme18I GGWCC 3 cut(s) 156, 244, 1116
BmgT120I GGNCC 6 cut(s) 119, 156, 244, 1071, 1116, 1122
BmiI GGNNCC 6 cut(s) 80, 127, 157, 246, 1118, 1124
BmsI GCATC 3 cut(s) 600, 1144, 1197
BmtI GCTAGC 1 cut(s) 1235
BplI GAGNNNNNCTC 4 cut(s) 8, 40, 221, 253
BpmI CTGGAG 3 cut(s) 363, 954, 1054
Bpu1102I GCTNAGC 1 cut(s) 20
BpuEI CTTGAG 2 cut(s) 480, 1478
BpuMI CCSGG 4 cut(s) 123, 130, 552, 1120
Bsa29I ATCGAT 1 cut(s) 1500
BsaHI GRCGYC 2 cut(s) 717, 1449
BsaI GGTCTC 1 cut(s) 74
BsaJI CCNNGG 5 cut(s) 33, 82, 1140, 1238, 1414
BsaWI WCCGGW 1 cut(s) 979
Bsc4I CCNNNNNNNGG 3 cut(s) 179, 416, 557
Bse118I RCCGGY 1 cut(s) 442
Bse1I ACTGG 1 cut(s) 1352
Bse3DI GCAATG 1 cut(s) 856
BseAI TCCGGA 1 cut(s) 979
BseBI CCWGG 6 cut(s) 83, 227, 607, 624, 933, 1274
BseCI ATCGAT 1 cut(s) 1500
BseDI CCNNGG 5 cut(s) 33, 82, 1140, 1238, 1414
BseGI GGATG 4 cut(s) 214, 525, 615, 1150
BseLI CCNNNNNNNGG 3 cut(s) 179, 416, 557
BseMI GCAATG 1 cut(s) 856
BseMII CTCAG 3 cut(s) 34, 231, 314
BseNI ACTGG 1 cut(s) 1352
BseRI GAGGAG 1 cut(s) 158
BseXI GCAGC 4 cut(s) 33, 1246, 1414, 1417
BsgI GTGCAG 1 cut(s) 1245
BshFI GGCC 6 cut(s) 121, 442, 470, 1073, 1123, 1243
BshNI GGYRCC 1 cut(s) 78
BshVI ATCGAT 1 cut(s) 1500
BsiHKAI GWGCWC 2 cut(s) 21, 234
BsiSI CCGG 6 cut(s) 122, 130, 443, 551, 980, 1120
BslFI GGGAC 4 cut(s) 230, 548, 574, 715
BslI CCNNNNNNNGG 3 cut(s) 179, 416, 557
BsmAI GTCTC 1 cut(s) 74
BsmFI GGGAC 4 cut(s) 230, 548, 574, 715
BsmI GAATGC 2 cut(s) 786, 1006
BsnI GGCC 6 cut(s) 121, 442, 470, 1073, 1123, 1243
Bso31I GGTCTC 1 cut(s) 74
Bsp1286I GDGCHC 3 cut(s) 21, 128, 234
Bsp13I TCCGGA 1 cut(s) 979
Bsp1407I TGTACA 1 cut(s) 948
Bsp1720I GCTNAGC 1 cut(s) 20
BspACI CCGC 7 cut(s) 173, 183, 374, 385, 407, 1257, 1265
BspANI GGCC 6 cut(s) 121, 442, 470, 1073, 1123, 1243
BspCNI CTCAG 3 cut(s) 33, 232, 315
BspDI ATCGAT 1 cut(s) 1500
BspEI TCCGGA 1 cut(s) 979
BspHI TCATGA 1 cut(s) 712
BspLI GGNNCC 6 cut(s) 80, 127, 157, 246, 1118, 1124
BspOI GCTAGC 1 cut(s) 1235
BspPI GGATC 3 cut(s) 129, 971, 1001
BspT107I GGYRCC 1 cut(s) 78
BspTNI GGTCTC 1 cut(s) 74
BsrBI CCGCTC 1 cut(s) 385
BsrDI GCAATG 1 cut(s) 856
BsrFI RCCGGY 1 cut(s) 442
BsrGI TGTACA 1 cut(s) 948
BsrI ACTGG 1 cut(s) 1352
BssAI RCCGGY 1 cut(s) 442
BssECI CCNNGG 5 cut(s) 33, 82, 1140, 1238, 1414
BssNI GRCGYC 2 cut(s) 717, 1449
BssT1I CCWWGG 3 cut(s) 1140, 1238, 1414
Bst2UI CCWGG 6 cut(s) 83, 227, 607, 624, 933, 1274
Bst4CI ACNGT 1 cut(s) 276
Bst6I CTCTTC 1 cut(s) 1204
BstACI GRCGYC 2 cut(s) 717, 1449
BstAUI TGTACA 1 cut(s) 948
BstC8I GCNNGC 8 cut(s) 17, 89, 257, 374, 444, 852, 1183, 1233
BstDEI CTNAG 4 cut(s) 20, 240, 323, 926
BstDSI CCRYGG 1 cut(s) 33
BstF5I GGATG 4 cut(s) 214, 525, 615, 1150
BstMAI GTCTC 1 cut(s) 74
BstMWI GCNNNNNNNGC 4 cut(s) 21, 373, 382, 1249
BstNI CCWGG 6 cut(s) 83, 227, 607, 624, 933, 1274
BstV1I GCAGC 4 cut(s) 33, 1246, 1414, 1417
BstX2I RGATCY 2 cut(s) 134, 993
BstYI RGATCY 2 cut(s) 134, 993
Bsu15I ATCGAT 1 cut(s) 1500
BsuRI GGCC 6 cut(s) 121, 442, 470, 1073, 1123, 1243
BsuTUI ATCGAT 1 cut(s) 1500
BtgI CCRYGG 1 cut(s) 33
BtsCI GGATG 4 cut(s) 214, 525, 615, 1150
BtsI GCAGTG 1 cut(s) 367
BtsIMutI CAGTG 2 cut(s) 367, 1079
Cac8I GCNNGC 8 cut(s) 17, 89, 257, 374, 444, 852, 1183, 1233
CciI TCATGA 1 cut(s) 712
Cfr10I RCCGGY 1 cut(s) 442
Cfr13I GGNCC 6 cut(s) 119, 156, 244, 1071, 1116, 1122
ClaI ATCGAT 1 cut(s) 1500
Csp6I GTAC 5 cut(s) 100, 589, 813, 949, 1345
CspCI CAANNNNNGTGG 2 cut(s) 625, 660
CviAII CATG 7 cut(s) 218, 572, 713, 752, 1174, 1396, 1478
CviQI GTAC 5 cut(s) 100, 589, 813, 949, 1345
DdeI CTNAG 4 cut(s) 20, 240, 323, 926
EaeI YGGCCR 2 cut(s) 440, 1241
Eam1104I CTCTTC 1 cut(s) 1204
EarI CTCTTC 1 cut(s) 1204
Ecl136II GAGCTC 1 cut(s) 19
Eco130I CCWWGG 3 cut(s) 1140, 1238, 1414
Eco24I GRGCYC 2 cut(s) 21, 128
Eco31I GGTCTC 1 cut(s) 74
Eco47I GGWCC 3 cut(s) 156, 244, 1116
Eco53kI GAGCTC 1 cut(s) 19
Eco57I CTGAAG 4 cut(s) 71, 981, 1315, 1317
EcoICRI GAGCTC 1 cut(s) 19
EcoO109I RGGNCCY 2 cut(s) 244, 1116
EcoRI GAATTC 1 cut(s) 1301
EcoRII CCWGG 6 cut(s) 81, 225, 605, 622, 931, 1272
EcoT14I CCWWGG 3 cut(s) 1140, 1238, 1414
EcoT38I GRGCYC 2 cut(s) 21, 128
ErhI CCWWGG 3 cut(s) 1140, 1238, 1414
FaeI CATG 7 cut(s) 221, 575, 716, 755, 1177, 1399, 1481
FalI AAGNNNNNCTT 2 cut(s) 811, 843
FaqI GGGAC 4 cut(s) 230, 548, 574, 715
FatI CATG 7 cut(s) 217, 571, 712, 751, 1173, 1395, 1477
FauI CCCGC 3 cut(s) 176, 180, 381
FauNDI CATATG 2 cut(s) 967, 1322
FblI GTMKAC 1 cut(s) 168
Fnu4HI GCNGC 5 cut(s) 47, 386, 1260, 1428, 1431
FokI GGATG 4 cut(s) 221, 532, 622, 1157
FriOI GRGCYC 2 cut(s) 21, 128
Fsp4HI GCNGC 5 cut(s) 47, 386, 1260, 1428, 1431
FspBI CTAG 4 cut(s) 776, 1113, 1232, 1317
GluI GCNGC 5 cut(s) 47, 386, 1260, 1428, 1431
GsuI CTGGAG 3 cut(s) 363, 954, 1054
HaeIII GGCC 6 cut(s) 121, 442, 470, 1073, 1123, 1243
HapII CCGG 6 cut(s) 122, 130, 443, 551, 980, 1120
Hin1I GRCGYC 2 cut(s) 717, 1449
Hin1II CATG 7 cut(s) 221, 575, 716, 755, 1177, 1399, 1481
HincII GTYRAC 1 cut(s) 169
HindII GTYRAC 1 cut(s) 169
HindIII AAGCTT 1 cut(s) 253
HinfI GANTC 6 cut(s) 165, 757, 897, 1109, 1136, 1491
HpaII CCGG 6 cut(s) 122, 130, 443, 551, 980, 1120
HphI GGTGA 2 cut(s) 638, 1468
Hpy166II GTNNAC 3 cut(s) 169, 949, 951
Hpy8I GTNNAC 3 cut(s) 169, 949, 951
Hpy99I CGWCG 1 cut(s) 143
HpyAV CCTTC 2 cut(s) 460, 1171
HpyCH4III ACNGT 1 cut(s) 276
HpyCH4IV ACGT 3 cut(s) 344, 717, 1449
HpyCH4V TGCA 8 cut(s) 217, 751, 773, 971, 1157, 1185, 1210, 1262
HpyF10VI GCNNNNNNNGC 4 cut(s) 21, 373, 382, 1249
HpyF3I CTNAG 4 cut(s) 20, 240, 323, 926
HpySE526I ACGT 3 cut(s) 344, 717, 1449
Hsp92I GRCGYC 2 cut(s) 717, 1449
Hsp92II CATG 7 cut(s) 221, 575, 716, 755, 1177, 1399, 1481
Kpn2I TCCGGA 1 cut(s) 979
KroI GCCGGC 1 cut(s) 442
KroNI GCCGGC 1 cut(s) 444
LmnI GCTCC 7 cut(s) 131, 229, 352, 578, 742, 1240, 1474
Lsp1109I GCAGC 4 cut(s) 33, 1246, 1414, 1417
LweI GCATC 3 cut(s) 600, 1144, 1197
MaeI CTAG 4 cut(s) 776, 1113, 1232, 1317
MaeII ACGT 3 cut(s) 344, 717, 1449
MaeIII GTNAC 2 cut(s) 1042, 1094
MbiI CCGCTC 1 cut(s) 385
MboII GAAGA 5 cut(s) 695, 1007, 1191, 1289, 1310
MflI RGATCY 2 cut(s) 134, 993
MhlI GDGCHC 3 cut(s) 21, 128, 234
MlsI TGGCCA 1 cut(s) 1243
MluNI TGGCCA 1 cut(s) 1243
MlyI GAGTC 2 cut(s) 174, 1485
MmeI TCCRAC 2 cut(s) 162, 1142
Mox20I TGGCCA 1 cut(s) 1243
MroI TCCGGA 1 cut(s) 979
MroNI GCCGGC 1 cut(s) 442
MscI TGGCCA 1 cut(s) 1243
MseI TTAA 4 cut(s) 329, 347, 615, 1100
MslI CAYNNNNRTG 2 cut(s) 570, 1400
Msp20I TGGCCA 1 cut(s) 1243
MspA1I CMGCKG 1 cut(s) 1259
MspI CCGG 6 cut(s) 122, 130, 443, 551, 980, 1120
Mva1269I GAATGC 2 cut(s) 786, 1006
MvaI CCWGG 6 cut(s) 83, 227, 607, 624, 933, 1274
MwoI GCNNNNNNNGC 4 cut(s) 21, 373, 382, 1249
NaeI GCCGGC 1 cut(s) 444
NciI CCSGG 4 cut(s) 123, 130, 552, 1120
NdeI CATATG 2 cut(s) 967, 1322
NgoMIV GCCGGC 1 cut(s) 442
NheI GCTAGC 1 cut(s) 1231
NlaIII CATG 7 cut(s) 221, 575, 716, 755, 1177, 1399, 1481
NlaIV GGNNCC 6 cut(s) 80, 127, 157, 246, 1118, 1124
PagI TCATGA 1 cut(s) 712
PcsI WCGNNNNNNNCGW 1 cut(s) 186
PctI GAATGC 2 cut(s) 786, 1006
PdiI GCCGGC 1 cut(s) 444
PfeI GAWTC 4 cut(s) 757, 897, 1109, 1136
PfoI TCCNGGA 2 cut(s) 128, 605
PkrI GCNGC 5 cut(s) 48, 387, 1261, 1429, 1432
PleI GAGTC 2 cut(s) 173, 1485
PpsI GAGTC 2 cut(s) 173, 1485
PpuMI RGGWCCY 2 cut(s) 244, 1116
PsiI TTATAA 2 cut(s) 393, 423
Psp124BI GAGCTC 1 cut(s) 21
Psp5II RGGWCCY 2 cut(s) 244, 1116
Psp6I CCWGG 6 cut(s) 81, 225, 605, 622, 931, 1272
PspGI CCWGG 6 cut(s) 81, 225, 605, 622, 931, 1272
PspN4I GGNNCC 6 cut(s) 80, 127, 157, 246, 1118, 1124
PspPI GGNCC 6 cut(s) 119, 156, 244, 1071, 1116, 1122
PspPPI RGGWCCY 2 cut(s) 244, 1116
PsuI RGATCY 2 cut(s) 134, 993
RsaI GTAC 5 cut(s) 101, 590, 814, 950, 1346
RsaNI GTAC 5 cut(s) 100, 589, 813, 949, 1345
RseI CAYNNNNRTG 2 cut(s) 570, 1400
SacI GAGCTC 1 cut(s) 21
SalI GTCGAC 1 cut(s) 167
SaqAI TTAA 4 cut(s) 329, 347, 615, 1100
SatI GCNGC 5 cut(s) 47, 386, 1260, 1428, 1431
Sau96I GGNCC 6 cut(s) 119, 156, 244, 1071, 1116, 1122
ScaI AGTACT 1 cut(s) 1346
SchI GAGTC 2 cut(s) 174, 1485
SduI GDGCHC 3 cut(s) 21, 128, 234
SfaNI GCATC 3 cut(s) 600, 1144, 1197
SinI GGWCC 3 cut(s) 156, 244, 1116
SmiMI CAYNNNNRTG 2 cut(s) 570, 1400
SmlI CTYRAG 2 cut(s) 495, 1493
SmoI CTYRAG 2 cut(s) 495, 1493
SsiI CCGC 7 cut(s) 173, 183, 374, 385, 407, 1257, 1265
SspMI CTAG 4 cut(s) 776, 1113, 1232, 1317
SstI GAGCTC 1 cut(s) 21
StyI CCWWGG 3 cut(s) 1140, 1238, 1414
TaaI ACNGT 1 cut(s) 276
TaiI ACGT 3 cut(s) 347, 720, 1452
TaqI TCGA 5 cut(s) 168, 666, 871, 1192, 1500
TaqII GACCGA 1 cut(s) 56
TatI WGTACW 4 cut(s) 99, 588, 948, 1344
TauI GCSGC 1 cut(s) 388
TfiI GAWTC 4 cut(s) 757, 897, 1109, 1136
Tru1I TTAA 4 cut(s) 329, 347, 615, 1100
Tru9I TTAA 4 cut(s) 329, 347, 615, 1100
TscAI CASTG 2 cut(s) 374, 1086
TseI GCWGC 4 cut(s) 46, 1259, 1427, 1430
TspDTI ATGAA 7 cut(s) 17, 471, 1190, 1373, 1418, 1455, 1475
TspGWI ACGGA 1 cut(s) 644
TspRI CASTG 2 cut(s) 374, 1086
VpaK11BI GGWCC 3 cut(s) 156, 244, 1116
XapI RAATTY 5 cut(s) 337, 617, 632, 1301, 1378
XmiI GTMKAC 1 cut(s) 168
XspI CTAG 4 cut(s) 776, 1113, 1232, 1317
ZraI GACGTC 2 cut(s) 718, 1450
ZrmI AGTACT 1 cut(s) 1346
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.