pycom17g04720

Neddylation of cullins play an essential role in the regulation of SCF-type complexes activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0013058
Physical Location & Seq
Reverse (-)
3248595 .. 3250372
1778 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 753 bp
ATGTTGCGTCTGGATTTGATGGCAGACTTTTCTGAATACTCACGTTTCTATGACTTTGTTTTCTTCATGTGCCGTGAAAATGGTCAGAAGAATATCACTGTAAATAAGGCAGTGACTGCATGGAGATTTGTCCTAGCTGGGAGGTTTCGGTTGCTGAATCAATGGTGTGATTTTGTTGAGAAAAATCAGCGGCATAACATCTCTGAAGATACATGGCGGCAAGTTTTAGCTTTTAGCCGCTGTGTGCATGAAAATCTGGAAGGGTATGATCCTGAAGGAGCTTGGCCTGTTTTAATAGATGACTTCGTTGAACATATGTACAGGGTTTTGGGATCTAACAATAACTCTAGCTTCAACTGTAACTGCGGTGATTCAGAATCCTGGTCATGTACATATGATGACCCTCTTCCTGGATTGAAGATTTTACCCGGTTTGAAGAGAAAGTTACCTGGGGACATGCAGATCGATGAAATGGTATCCTCACCCACCCTGTTCCCACACTGTACAGACTGGAATCCTGCATTAAGCACTAAAAAAAGTCGTGCGATGTCATGCATACCAGCAAACTGGGACGATACTACATCAGGAAATACTGTGGATGATTCCATGGAAACCGTAAGACATAGCAGTCCACTGAATTCTTCAAAATCTCCATGCGCGGTTGAAGGTTGCCTGTTGAAGGGCTTTGCAGGACTGCTTTCTACACGTTCCTGTCTGCAGTTTGATCGGGAGAGGGGAGTTTCATTTTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cullin_binding

Protein Analysis

251

Amino Acids

28.45

Weight (kDa)

5.41

Isoelectric Point (pI)

50.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cullin_binding PF03556 8 - 105 1.1e-29 Cullin binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013058)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G28970 AT3G28970
fragaria_vesca FvH4_6g48370
malus_domestica MD09G1052100.v1.1
prunus_persica Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1 Prupe.3G266600_v2.0.a1
pyrus_communis pycom17g04720
rosa_chinensis RchiOBHm_Chr2g0168311
rosa_laevigata RLG00000021765
rosa_roxburghii Rroxscaffold_2G00083160
rosa_rugosa Rorug02G0532600
rosa_samantha Rh2AG601900 Rh2BG615100 Rh2CG582800 Rh2DG625300
rosa_wichuraiana Rw2G049950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 627
AccII CGCG 1 cut(s) 659
AciI CCGC 5 cut(s) 190, 217, 238, 366, 659
AclWI GGATC 2 cut(s) 263, 340
AcsI RAATTY 1 cut(s) 637
AcuI CTGAAG 2 cut(s) 225, 294
AfaI GTAC 3 cut(s) 320, 391, 505
AfiI CCNNNNNNNGG 2 cut(s) 410, 679
AflIII ACRYGT 1 cut(s) 704
AgsI TTSAA 7 cut(s) 311, 355, 418, 436, 645, 665, 679
AjnI CCWGG 3 cut(s) 380, 409, 448
AluBI AGCT 4 cut(s) 137, 230, 281, 351
AluI AGCT 4 cut(s) 137, 230, 281, 351
AlwI GGATC 2 cut(s) 263, 340
AlwNI CAGNNNCTG 1 cut(s) 116
AoxI GGCC 1 cut(s) 284
ApoI RAATTY 1 cut(s) 637
AspLEI GCGC 1 cut(s) 659
AsuC2I CCSGG 1 cut(s) 429
AsuHPI GGTGA 2 cut(s) 380, 474
BccI CCATC 1 cut(s) 13
BceAI ACGGC 1 cut(s) 57
BcgI CGANNNNNNTGC 2 cut(s) 707, 741
BciT130I CCWGG 3 cut(s) 382, 411, 450
BciVI GTATCC 1 cut(s) 487
BcnI CCSGG 1 cut(s) 429
BfaI CTAG 2 cut(s) 134, 348
BfmI CTRYAG 1 cut(s) 716
BfuI GTATCC 1 cut(s) 487
BisI GCNGC 3 cut(s) 191, 218, 238
BlsI GCNGC 3 cut(s) 192, 219, 239
Bme1390I CCNGG 4 cut(s) 382, 411, 429, 450
BmrFI CCNGG 4 cut(s) 382, 411, 429, 450
BmrI ACTGGG 1 cut(s) 577
BmuI ACTGGG 1 cut(s) 577
BpuMI CCSGG 1 cut(s) 429
Bsa29I ATCGAT 1 cut(s) 465
BsaJI CCNNGG 2 cut(s) 449, 606
Bsc4I CCNNNNNNNGG 2 cut(s) 410, 679
Bse1I ACTGG 2 cut(s) 515, 572
BseBI CCWGG 3 cut(s) 382, 411, 450
BseCI ATCGAT 1 cut(s) 465
BseDI CCNNGG 2 cut(s) 449, 606
BseGI GGATG 1 cut(s) 604
BseLI CCNNNNNNNGG 2 cut(s) 410, 679
BseNI ACTGG 2 cut(s) 515, 572
BseYI CCCAGC 1 cut(s) 137
Bsh1236I CGCG 1 cut(s) 659
BshFI GGCC 1 cut(s) 286
BshVI ATCGAT 1 cut(s) 465
BsiSI CCGG 1 cut(s) 429
BslFI GGGAC 2 cut(s) 467, 584
BslI CCNNNNNNNGG 2 cut(s) 410, 679
BsmFI GGGAC 2 cut(s) 467, 584
BsnI GGCC 1 cut(s) 286
Bsp1407I TGTACA 3 cut(s) 318, 389, 503
Bsp143I GATC 4 cut(s) 268, 332, 462, 724
Bsp19I CCATGG 1 cut(s) 606
BspACI CCGC 5 cut(s) 190, 217, 238, 366, 659
BspANI GGCC 1 cut(s) 286
BspDI ATCGAT 1 cut(s) 465
BspFNI CGCG 1 cut(s) 659
BspMAI CTGCAG 1 cut(s) 720
BspPI GGATC 2 cut(s) 263, 340
BsrGI TGTACA 3 cut(s) 318, 389, 503
BsrI ACTGG 2 cut(s) 515, 572
BssECI CCNNGG 2 cut(s) 449, 606
BssMI GATC 4 cut(s) 268, 332, 462, 724
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 3 cut(s) 382, 411, 450
Bst4CI ACNGT 5 cut(s) 100, 359, 503, 595, 616
Bst6I CTCTTC 2 cut(s) 411, 431
BstAPI GCANNNNNTGC 1 cut(s) 116
BstAUI TGTACA 3 cut(s) 318, 389, 503
BstDSI CCRYGG 1 cut(s) 606
BstENI CCTNNNNNAGG 1 cut(s) 677
BstF5I GGATG 1 cut(s) 604
BstFNI CGCG 1 cut(s) 659
BstHHI GCGC 1 cut(s) 659
BstKTI GATC 4 cut(s) 271, 335, 465, 727
BstMBI GATC 4 cut(s) 268, 332, 462, 724
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 3 cut(s) 382, 411, 450
BstNSI RCATGY 1 cut(s) 460
BstSCI CCNGG 4 cut(s) 380, 409, 427, 448
BstSFI CTRYAG 1 cut(s) 716
BstUI CGCG 1 cut(s) 659
BstX2I RGATCY 1 cut(s) 332
BstXI CCANNNNNNTGG 1 cut(s) 567
BstYI RGATCY 1 cut(s) 332
Bsu15I ATCGAT 1 cut(s) 465
BsuI GTATCC 1 cut(s) 487
BsuRI GGCC 1 cut(s) 286
BsuTUI ATCGAT 1 cut(s) 465
BtgI CCRYGG 1 cut(s) 606
BtgZI GCGATG 1 cut(s) 560
BtsCI GGATG 1 cut(s) 604
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 4 cut(s) 96, 117, 499, 632
CaiI CAGNNNCTG 1 cut(s) 116
CfoI GCGC 1 cut(s) 659
ClaI ATCGAT 1 cut(s) 465
Csp6I GTAC 3 cut(s) 319, 390, 504
CviAII CATG 9 cut(s) 67, 120, 213, 248, 387, 457, 552, 607, 654
CviJI RGCY 7 cut(s) 137, 230, 237, 281, 286, 351, 684
CviKI_1 RGCY 7 cut(s) 137, 230, 237, 281, 286, 351, 684
CviQI GTAC 3 cut(s) 319, 390, 504
DpnI GATC 4 cut(s) 270, 334, 464, 726
DpnII GATC 4 cut(s) 268, 332, 462, 724
DrdI GACNNNNNNGTC 1 cut(s) 627
DseDI GACNNNNNNGTC 1 cut(s) 627
Eam1104I CTCTTC 2 cut(s) 411, 431
EarI CTCTTC 2 cut(s) 411, 431
Eco130I CCWWGG 1 cut(s) 606
Eco57I CTGAAG 2 cut(s) 225, 294
EcoNI CCTNNNNNAGG 1 cut(s) 677
EcoRI GAATTC 1 cut(s) 637
EcoRII CCWGG 3 cut(s) 380, 409, 448
EcoT14I CCWWGG 1 cut(s) 606
EcoT22I ATGCAT 1 cut(s) 557
ErhI CCWWGG 1 cut(s) 606
FaeI CATG 9 cut(s) 70, 123, 216, 251, 390, 460, 555, 610, 657
FaqI GGGAC 2 cut(s) 467, 584
FatI CATG 9 cut(s) 66, 119, 212, 247, 386, 456, 551, 606, 653
FauNDI CATATG 2 cut(s) 315, 394
Fnu4HI GCNGC 3 cut(s) 191, 218, 238
FokI GGATG 1 cut(s) 611
Fsp4HI GCNGC 3 cut(s) 191, 218, 238
FspBI CTAG 2 cut(s) 134, 348
GlaI GCGC 1 cut(s) 658
GluI GCNGC 3 cut(s) 191, 218, 238
GsaI CCCAGC 1 cut(s) 141
HaeIII GGCC 1 cut(s) 286
HapII CCGG 1 cut(s) 429
HhaI GCGC 1 cut(s) 659
Hin1II CATG 9 cut(s) 70, 123, 216, 251, 390, 460, 555, 610, 657
Hin6I GCGC 1 cut(s) 657
HinP1I GCGC 1 cut(s) 657
HinfI GANTC 5 cut(s) 157, 371, 377, 514, 602
HpaII CCGG 1 cut(s) 429
HphI GGTGA 2 cut(s) 380, 474
Hpy166II GTNNAC 1 cut(s) 632
Hpy188I TCNGA 4 cut(s) 34, 87, 205, 376
Hpy188III TCNNGA 5 cut(s) 11, 257, 272, 585, 728
Hpy8I GTNNAC 1 cut(s) 632
HpyAV CCTTC 4 cut(s) 254, 269, 659, 673
HpyCH4III ACNGT 5 cut(s) 100, 359, 503, 595, 616
HpyCH4IV ACGT 2 cut(s) 43, 706
HpyCH4V TGCA 7 cut(s) 119, 247, 460, 521, 555, 689, 718
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpySE526I ACGT 2 cut(s) 43, 706
Hsp92II CATG 9 cut(s) 70, 123, 216, 251, 390, 460, 555, 610, 657
HspAI GCGC 1 cut(s) 657
Kzo9I GATC 4 cut(s) 268, 332, 462, 724
LmnI GCTCC 1 cut(s) 278
MaeI CTAG 2 cut(s) 134, 348
MaeII ACGT 2 cut(s) 43, 706
MaeIII GTNAC 3 cut(s) 112, 359, 444
MalI GATC 4 cut(s) 270, 334, 464, 726
MboI GATC 4 cut(s) 268, 332, 462, 724
MboII GAAGA 7 cut(s) 55, 100, 218, 398, 430, 448, 633
MflI RGATCY 1 cut(s) 332
MluCI AATT 1 cut(s) 637
MnlI CCTC 4 cut(s) 135, 414, 490, 726
Mph1103I ATGCAT 1 cut(s) 557
MseI TTAA 2 cut(s) 293, 524
MspA1I CMGCKG 2 cut(s) 190, 240
MspI CCGG 1 cut(s) 429
MspR9I CCNGG 4 cut(s) 382, 411, 429, 450
MvaI CCWGG 3 cut(s) 382, 411, 450
MvnI CGCG 1 cut(s) 659
MwoI GCNNNNNNNGC 1 cut(s) 116
NciI CCSGG 1 cut(s) 429
NcoI CCATGG 1 cut(s) 606
NdeI CATATG 2 cut(s) 315, 394
NdeII GATC 4 cut(s) 268, 332, 462, 724
NlaIII CATG 9 cut(s) 70, 123, 216, 251, 390, 460, 555, 610, 657
NmuCI GTSAC 1 cut(s) 112
NsiI ATGCAT 1 cut(s) 557
NspI RCATGY 1 cut(s) 460
PfeI GAWTC 5 cut(s) 157, 371, 377, 514, 602
PfoI TCCNGGA 1 cut(s) 409
PkrI GCNGC 3 cut(s) 192, 219, 239
Psp6I CCWGG 3 cut(s) 380, 409, 448
PspFI CCCAGC 1 cut(s) 137
PspGI CCWGG 3 cut(s) 380, 409, 448
PstI CTGCAG 1 cut(s) 720
PstNI CAGNNNCTG 1 cut(s) 116
PsuI RGATCY 1 cut(s) 332
RsaI GTAC 3 cut(s) 320, 391, 505
RsaNI GTAC 3 cut(s) 319, 390, 504
SaqAI TTAA 2 cut(s) 293, 524
SatI GCNGC 3 cut(s) 191, 218, 238
Sau3AI GATC 4 cut(s) 268, 332, 462, 724
ScrFI CCNGG 4 cut(s) 382, 411, 429, 450
SetI ASST 9 cut(s) 46, 139, 146, 232, 283, 353, 451, 670, 709
SfcI CTRYAG 1 cut(s) 716
Sse9I AATT 1 cut(s) 637
SsiI CCGC 5 cut(s) 190, 217, 238, 366, 659
SspMI CTAG 2 cut(s) 134, 348
StyD4I CCNGG 4 cut(s) 380, 409, 427, 448
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 5 cut(s) 100, 359, 503, 595, 616
TaiI ACGT 2 cut(s) 46, 709
TaqI TCGA 1 cut(s) 465
TasI AATT 1 cut(s) 637
TatI WGTACW 3 cut(s) 318, 389, 503
TauI GCSGC 3 cut(s) 193, 220, 240
TfiI GAWTC 5 cut(s) 157, 371, 377, 514, 602
Tru1I TTAA 2 cut(s) 293, 524
Tru9I TTAA 2 cut(s) 293, 524
TscAI CASTG 4 cut(s) 103, 117, 506, 639
TseFI GTSAC 1 cut(s) 112
Tsp45I GTSAC 1 cut(s) 112
TspDTI ATGAA 4 cut(s) 55, 264, 483, 732
TspRI CASTG 4 cut(s) 103, 117, 506, 639
XagI CCTNNNNNAGG 1 cut(s) 677
XapI RAATTY 1 cut(s) 637
XceI RCATGY 1 cut(s) 460
XspI CTAG 2 cut(s) 134, 348
Zsp2I ATGCAT 1 cut(s) 557
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.