pycom17g05750

Belongs to the peptidase M16 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0015224
Physical Location & Seq
Forward (+)
4064868 .. 4068466
3599 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1692 bp
ATGGCTGAGCCTCTGCAATCGCAGTTCGCACTTCTTTGCCGTATTTCGTTTCCCCCAAACCGAAACCCTAGAAGGCCTCTTACTGAGACAGCCATGGCGATGAAGCACCTTCTTACTACCCTAGCTCGCCGCCGGCCCCATAGACCGCCGTCTGCTCTCACCGCAGCCGTCCGATCCCAATCGACCTCACCCGCCGTCGCTACGTCACCTTCATCCCCATCCCTCCCCTCCCCTCCTCCACCAACCGCCATGATCTACGATCGATTGGCCGAGGGCGTCAAATCGAAGATCAAAAGGCTCGAGAACCCGGACCCGAGGTTCCTTAAGTACGGGTCGCCCCACCCGAGTCTCACGGACCACAGCCACATCCTCACCGCACCCGAGACACGTGTCACCACACTCCCCAACGGCCTTCGGGTGGCCACCGAGTCGAACCTCGCGTCTAAGACCGCCACTGTTGGAGTCTGGATCGACGCCGGGTCAAGGTTCGAAGACGACGAGACCAACGGCACTGCGCATTTCCTGGAGCACATGATCTTCAAGGGGACGGAGCAGAGGACGGCCCGTGATCTGGAGGAGGAGGTCGAGAACATGGGAGGTCACTTGAATGCGTACACATCGAGGGAGCAGACCACGTACTATGCCAAGGTGCTCGATTCCGATGTGCCGAAGGCCCTTTCGATTTTGGCTGATATCTTGCAGAACTCCAAGTTCGATGAGGACCGGATTTTGCGCGAGCGCGATGTGATTTTGAGGGAAATGGAGGAGGTCGAGAAACAACCCGAAGAAGTCATTTTTGACCATCTTCATGCAACTGCATTCCAGTACTCTCCTCTAGCCAGAACTATTCTTGGACCTGCTAACAACATTAAGACCATCTCCAAAGAGCATCTTCAGAACTATATCAAAACACATTACACTGCTCCCAGAATGGTTATTGCTGCTTCAGGAGCTATCAAGCATGAAGATCTTGTTGGGTCAGTTAAGGGTTTGTTCACAAAACTCTCAGGAGATCCTACCACTGCCGGTCAGTTGGTTGCGAAAGAACCTTCATTTTTTACCGGTTCTGAGGTCAGAATTGTGGATGATGACTTGCCTTTGGCACATTTTGCGGTTGCGTTCAGTGGTGCATCTTGGACTGATCCCGACTCTATTCCTCTAATGGTCATGCAAGCGATGCTGGGTTCATGGAATAAAAATGCTGGTGGTGGAAAGCACATGGGTTCAGAACTGGCACAGAGGGTTGCCATTAATGACATAGCAGAAAGCTATATGTCTTTCAACACCAACTATAAGGATACTGGCTTATTCGGTGTTTTTGCTACTGCTCCGGCGGATTGCTTAGATGATTTGGCCTATGCAATCATGTATGAGGTCACCAAGTTAGTTTATCGAGTTTCAGAAGCTGATGTTATTCGTGCTCGTAATCAGTTGAAATCTTCGCTGCTCCTGCATTTGGATGGGACAAGTGCCGTAGCTGAAGATATTGGGCGTCAGTTACTTACATATGGTCGAAGAATCCCCTTACCCGAGCTGTTTGCTAGAATTGATTCTGTTGATGCAAGCACCATCAAACGTGTTGCCAACCGATTCATCTATGACAGGGATATCACAATTGCAGCCATGGGTCCGATTACAAAATTGCCCGACTACAACTGGTTCAGACGCCGCACCTATTGGAACAGATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004175 GO:0004222 GO:0005488 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005739 GO:0005740 GO:0005741 GO:0005743 GO:0005746 GO:0005750 GO:0005758 GO:0005759 GO:0005773 GO:0005774 GO:0006091 GO:0006119 GO:0006122 GO:0006139 GO:0006163 GO:0006508 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008237 GO:0008270 GO:0009060 GO:0009117 GO:0009123 GO:0009126 GO:0009141 GO:0009144 GO:0009150 GO:0009161 GO:0009167 GO:0009199 GO:0009205 GO:0009259 GO:0009507 GO:0009536 GO:0009987 GO:0010467 GO:0015980 GO:0016020 GO:0016310 GO:0016485 GO:0016787 GO:0017144 GO:0019538 GO:0019637 GO:0019693 GO:0019866 GO:0019867 GO:0022900 GO:0022904 GO:0030312 GO:0031090 GO:0031966 GO:0031967 GO:0031968 GO:0031970 GO:0031974 GO:0031975 GO:0031981 GO:0032991 GO:0034641 GO:0042773 GO:0042775 GO:0043167 GO:0043169 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044425 GO:0044428 GO:0044429 GO:0044437 GO:0044444 GO:0044446 GO:0044455 GO:0044464 GO:0045275 GO:0045333 GO:0046034 GO:0046483 GO:0046872 GO:0046914 GO:0051604 GO:0055086 GO:0055114 GO:0070011 GO:0070013 GO:0070069 GO:0070469 GO:0071704 GO:0071944 GO:0072521 GO:0098588 GO:0098796 GO:0098798 GO:0098800 GO:0098803 GO:0098805 GO:0140096 GO:1901135 GO:1901360 GO:1901564 GO:1902494 GO:1990204
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_M16 Peptidase_M16_C

Protein Analysis

564

Amino Acids

62.67

Weight (kDa)

6.78

Isoelectric Point (pI)

48.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M16 PF00675 139 - 286 1.7e-55 Insulinase (Peptidase family M16)
Peptidase_M16_C PF05193 293 - 478 1.1e-35 Peptidase M16 inactive domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015224)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G02090 AT3G02090
fragaria_vesca FvH4_6g47100
malus_domestica MD09G1064100.v1.1 MD17G1057400.v1.1
prunus_persica Prupe.3G256900_v2.0.a1
pyrus_communis pycom111g05370 pycom111g05380 pycom17g05750
rosa_chinensis RchiOBHm_Chr2g0166151
rosa_laevigata RLG00000021590
rosa_multiflora Rmu_sc0006218.1_g000030
rosa_roxburghii Rroxscaffold_2G00085160
rosa_rugosa Rorug02G0520700
rosa_samantha Rh2BG598600
rosa_wichuraiana Rw2G048920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 516
Acc36I ACCTGC 1 cut(s) 865
AccII CGCG 3 cut(s) 440, 735, 741
AclWI GGATC 4 cut(s) 168, 476, 1007, 1136
AcoI YGGCCR 2 cut(s) 267, 420
AcuI CTGAAG 3 cut(s) 878, 930, 1500
AcvI CACGTG 1 cut(s) 389
AcyI GRCGYC 4 cut(s) 276, 474, 1492, 1666
AfaI GTAC 4 cut(s) 329, 614, 638, 827
AfiI CCNNNNNNNGG 4 cut(s) 418, 483, 571, 1456
AflII CTTAAG 1 cut(s) 323
AflIII ACRYGT 3 cut(s) 386, 388, 1576
AgeI ACCGGT 1 cut(s) 1061
AgsI TTSAA 4 cut(s) 541, 607, 1282, 1435
AhdI GACNNNNNGTC 1 cut(s) 478
AjnI CCWGG 1 cut(s) 522
AjuI GAANNNNNNNTTGG 2 cut(s) 957, 989
AloI GAACNNNNNNTCC 2 cut(s) 302, 334
AluBI AGCT 6 cut(s) 125, 953, 1269, 1406, 1478, 1534
AluI AGCT 6 cut(s) 125, 953, 1269, 1406, 1478, 1534
Alw21I GWGCWC 3 cut(s) 531, 654, 1423
Alw26I GTCTC 4 cut(s) 80, 353, 377, 494
AlwI GGATC 4 cut(s) 168, 476, 1007, 1136
AlwNI CAGNNNCTG 1 cut(s) 1406
Ama87I CYCGRG 5 cut(s) 299, 313, 343, 380, 1529
AoxI GGCC 8 cut(s) 74, 134, 267, 409, 420, 561, 672, 1353
ApeKI GCWGC 4 cut(s) 164, 941, 1444, 1619
ArsI GACNNNNNNTTYG 2 cut(s) 425, 457
AseI ATTAAT 1 cut(s) 1251
AsiGI ACCGGT 1 cut(s) 1061
Asp700I GAANNNNTTC 1 cut(s) 1549
AspLEI GCGC 3 cut(s) 517, 735, 741
AspS9I GGNCC 8 cut(s) 135, 310, 355, 562, 673, 721, 854, 1628
AsuC2I CCSGG 2 cut(s) 308, 478
AsuHPI GGTGA 6 cut(s) 151, 180, 198, 364, 385, 1369
AsuII TTCGAA 1 cut(s) 489
AvaI CYCGRG 5 cut(s) 299, 313, 343, 380, 1529
AvaII GGWCC 5 cut(s) 310, 355, 721, 854, 1628
BalI TGGCCA 1 cut(s) 422
BarI GAAGNNNNNNTAC 2 cut(s) 64, 96
BbrPI CACGTG 1 cut(s) 389
BbsI GAAGAC 1 cut(s) 498
Bbv12I GWGCWC 3 cut(s) 531, 654, 1423
BbvI GCAGC 4 cut(s) 176, 928, 1431, 1631
BccI CCATC 5 cut(s) 226, 810, 884, 1454, 1577
BceAI ACGGC 8 cut(s) 24, 133, 152, 179, 424, 523, 576, 1457
BcgI CGANNNNNNTGC 2 cut(s) 1520, 1554
BciT130I CCWGG 1 cut(s) 524
BciVI GTATCC 1 cut(s) 1291
BcnI CCSGG 2 cut(s) 308, 478
BcoDI GTCTC 4 cut(s) 80, 353, 377, 494
BfaI CTAG 4 cut(s) 69, 122, 836, 1542
BfrI CTTAAG 1 cut(s) 323
BfuAI ACCTGC 1 cut(s) 865
BfuI GTATCC 1 cut(s) 1291
BglII AGATCT 1 cut(s) 967
BisI GCNGC 6 cut(s) 130, 165, 942, 1445, 1620, 1669
BlpI GCTNAGC 1 cut(s) 6
BlsI GCNGC 6 cut(s) 131, 166, 943, 1446, 1621, 1670
BmcAI AGTACT 1 cut(s) 827
Bme1390I CCNGG 3 cut(s) 308, 478, 524
Bme18I GGWCC 5 cut(s) 310, 355, 721, 854, 1628
BmeRI GACNNNNNGTC 1 cut(s) 478
BmeT110I CYCGRG 5 cut(s) 299, 313, 343, 380, 1529
BmgT120I GGNCC 8 cut(s) 135, 310, 355, 562, 673, 721, 854, 1628
BmiI GGNNCC 4 cut(s) 137, 312, 320, 1629
BmrFI CCNGG 3 cut(s) 308, 478, 524
BmsI GCATC 4 cut(s) 898, 1139, 1167, 1549
BoxI GACNNNNGTC 2 cut(s) 148, 389
BpiI GAAGAC 1 cut(s) 498
BpmI CTGGAG 2 cut(s) 545, 593
Bpu1102I GCTNAGC 1 cut(s) 6
Bpu14I TTCGAA 1 cut(s) 489
BpuMI CCSGG 2 cut(s) 308, 478
Bsa29I ATCGAT 1 cut(s) 262
BsaAI YACGTR 2 cut(s) 389, 636
BsaBI GATNNNNATC 1 cut(s) 178
BsaHI GRCGYC 4 cut(s) 276, 474, 1492, 1666
BsaI GGTCTC 1 cut(s) 494
BsaJI CCNNGG 5 cut(s) 93, 270, 314, 645, 1623
BsaWI WCCGGW 2 cut(s) 723, 1061
Bsc4I CCNNNNNNNGG 4 cut(s) 418, 483, 571, 1456
Bse118I RCCGGY 3 cut(s) 132, 1025, 1061
Bse1I ACTGG 4 cut(s) 823, 1236, 1306, 1661
Bse8I GATNNNNATC 1 cut(s) 178
BseBI CCWGG 1 cut(s) 524
BseCI ATCGAT 1 cut(s) 262
BseDI CCNNGG 5 cut(s) 93, 270, 314, 645, 1623
BseGI GGATG 5 cut(s) 212, 218, 366, 1090, 1465
BseJI GATNNNNATC 1 cut(s) 178
BseLI CCNNNNNNNGG 4 cut(s) 418, 483, 571, 1456
BseMII CTCAG 3 cut(s) 75, 1020, 1059
BseNI ACTGG 4 cut(s) 823, 1236, 1306, 1661
BseRI GAGGAG 5 cut(s) 225, 590, 593, 779, 822
BseXI GCAGC 4 cut(s) 176, 928, 1431, 1631
BseYI CCCAGC 1 cut(s) 1180
Bsh1236I CGCG 3 cut(s) 440, 735, 741
Bsh1285I CGRYCG 1 cut(s) 262
BshFI GGCC 8 cut(s) 76, 136, 269, 411, 422, 563, 674, 1355
BshTI ACCGGT 1 cut(s) 1061
BshVI ATCGAT 1 cut(s) 262
BsiEI CGRYCG 1 cut(s) 262
BsiHKAI GWGCWC 3 cut(s) 531, 654, 1423
BsiHKCI CYCGRG 5 cut(s) 299, 313, 343, 380, 1529
BsiSI CCGG 7 cut(s) 133, 308, 477, 724, 1026, 1062, 1331
BslFI GGGAC 2 cut(s) 559, 1477
BslI CCNNNNNNNGG 4 cut(s) 418, 483, 571, 1456
BsmAI GTCTC 4 cut(s) 80, 353, 377, 494
BsmFI GGGAC 2 cut(s) 559, 1477
BsmI GAATGC 2 cut(s) 613, 818
BsnI GGCC 8 cut(s) 76, 136, 269, 411, 422, 563, 674, 1355
Bso31I GGTCTC 1 cut(s) 494
BsoBI CYCGRG 5 cut(s) 299, 313, 343, 380, 1529
Bsp119I TTCGAA 1 cut(s) 489
Bsp1286I GDGCHC 3 cut(s) 531, 654, 1423
Bsp1720I GCTNAGC 1 cut(s) 6
Bsp19I CCATGG 2 cut(s) 93, 1623
BspANI GGCC 8 cut(s) 76, 136, 269, 411, 422, 563, 674, 1355
BspCNI CTCAG 3 cut(s) 76, 1019, 1060
BspDI ATCGAT 1 cut(s) 262
BspFNI CGCG 3 cut(s) 440, 735, 741
BspLI GGNNCC 4 cut(s) 137, 312, 320, 1629
BspMI ACCTGC 1 cut(s) 865
BspPI GGATC 4 cut(s) 168, 476, 1007, 1136
BspT104I TTCGAA 1 cut(s) 489
BspTI CTTAAG 1 cut(s) 323
BspTNI GGTCTC 1 cut(s) 494
BsrFI RCCGGY 3 cut(s) 132, 1025, 1061
BsrI ACTGG 4 cut(s) 823, 1236, 1306, 1661
BssAI RCCGGY 3 cut(s) 132, 1025, 1061
BssECI CCNNGG 5 cut(s) 93, 270, 314, 645, 1623
BssNI GRCGYC 4 cut(s) 276, 474, 1492, 1666
BssT1I CCWWGG 3 cut(s) 93, 645, 1623
Bst2UI CCWGG 1 cut(s) 524
Bst4CI ACNGT 1 cut(s) 457
BstACI GRCGYC 4 cut(s) 276, 474, 1492, 1666
BstAFI CTTAAG 1 cut(s) 323
BstAPI GCANNNNNTGC 2 cut(s) 1109, 1177
BstBAI YACGTR 2 cut(s) 389, 636
BstBI TTCGAA 1 cut(s) 489
BstC8I GCNNGC 5 cut(s) 127, 134, 737, 1173, 1564
BstDEI CTNAG 6 cut(s) 6, 84, 444, 1006, 1068, 1342
BstDSI CCRYGG 2 cut(s) 93, 1623
BstEII GGTNACC 1 cut(s) 1375
BstF5I GGATG 5 cut(s) 212, 218, 366, 1090, 1465
BstFNI CGCG 3 cut(s) 440, 735, 741
BstHHI GCGC 3 cut(s) 517, 735, 741
BstMAI GTCTC 4 cut(s) 80, 353, 377, 494
BstMCI CGRYCG 1 cut(s) 262
BstMWI GCNNNNNNNGC 5 cut(s) 161, 950, 1109, 1177, 1450
BstNI CCWGG 1 cut(s) 524
BstPAI GACNNNNGTC 2 cut(s) 148, 389
BstPI GGTNACC 1 cut(s) 1375
BstSCI CCNGG 3 cut(s) 306, 476, 522
BstUI CGCG 3 cut(s) 440, 735, 741
BstV1I GCAGC 4 cut(s) 176, 928, 1431, 1631
BstV2I GAAGAC 1 cut(s) 498
BstX2I RGATCY 2 cut(s) 967, 1012
BstYI RGATCY 2 cut(s) 967, 1012
Bsu15I ATCGAT 1 cut(s) 262
BsuI GTATCC 1 cut(s) 1291
BsuRI GGCC 8 cut(s) 76, 136, 269, 411, 422, 563, 674, 1355
BsuTUI ATCGAT 1 cut(s) 262
BtgI CCRYGG 2 cut(s) 93, 1623
BtgZI GCGATG 3 cut(s) 113, 756, 1190
BtsCI GGATG 5 cut(s) 212, 218, 366, 1090, 1465
BtsI GCAGTG 3 cut(s) 510, 918, 1020
BtsIMutI CAGTG 5 cut(s) 453, 510, 918, 1020, 1129
BveI ACCTGC 1 cut(s) 865
Cac8I GCNNGC 5 cut(s) 127, 134, 737, 1173, 1564
CaiI CAGNNNCTG 1 cut(s) 1406
CfoI GCGC 3 cut(s) 517, 735, 741
Cfr10I RCCGGY 3 cut(s) 132, 1025, 1061
Cfr13I GGNCC 8 cut(s) 135, 310, 355, 562, 673, 721, 854, 1628
ClaI ATCGAT 1 cut(s) 262
CseI GACGC 5 cut(s) 265, 429, 482, 1481, 1674
Csp6I GTAC 4 cut(s) 328, 613, 637, 826
CspAI ACCGGT 1 cut(s) 1061
CviQI GTAC 4 cut(s) 328, 613, 637, 826
DdeI CTNAG 6 cut(s) 6, 84, 444, 1006, 1068, 1342
DriI GACNNNNNGTC 1 cut(s) 478
EaeI YGGCCR 2 cut(s) 267, 420
Eam1105I GACNNNNNGTC 1 cut(s) 478
EciI GGCGGA 1 cut(s) 1349
Eco130I CCWWGG 3 cut(s) 93, 645, 1623
Eco147I AGGCCT 1 cut(s) 76
Eco31I GGTCTC 1 cut(s) 494
Eco32I GATATC 2 cut(s) 694, 1609
Eco47I GGWCC 5 cut(s) 310, 355, 721, 854, 1628
Eco57I CTGAAG 3 cut(s) 878, 930, 1500
Eco72I CACGTG 1 cut(s) 389
Eco88I CYCGRG 5 cut(s) 299, 313, 343, 380, 1529
Eco91I GGTNACC 1 cut(s) 1375
EcoO109I RGGNCCY 1 cut(s) 673
EcoO65I GGTNACC 1 cut(s) 1375
EcoRII CCWGG 1 cut(s) 522
EcoRV GATATC 2 cut(s) 694, 1609
EcoT14I CCWWGG 3 cut(s) 93, 645, 1623
ErhI CCWWGG 3 cut(s) 93, 645, 1623
FalI AAGNNNNNCTT 2 cut(s) 876, 908
FaqI GGGAC 2 cut(s) 559, 1477
FauI CCCGC 1 cut(s) 199
FauNDI CATATG 1 cut(s) 1507
Fnu4HI GCNGC 6 cut(s) 130, 165, 942, 1445, 1620, 1669
FokI GGATG 5 cut(s) 199, 205, 353, 1097, 1472
Fsp4HI GCNGC 6 cut(s) 130, 165, 942, 1445, 1620, 1669
FspBI CTAG 4 cut(s) 69, 122, 836, 1542
FspI TGCGCA 1 cut(s) 516
GlaI GCGC 3 cut(s) 516, 734, 740
GluI GCNGC 6 cut(s) 130, 165, 942, 1445, 1620, 1669
GsaI CCCAGC 1 cut(s) 1184
GsuI CTGGAG 2 cut(s) 545, 593
HaeIII GGCC 8 cut(s) 76, 136, 269, 411, 422, 563, 674, 1355
HapII CCGG 7 cut(s) 133, 308, 477, 724, 1026, 1062, 1331
HgaI GACGC 5 cut(s) 265, 429, 482, 1481, 1674
HhaI GCGC 3 cut(s) 517, 735, 741
Hin1I GRCGYC 4 cut(s) 276, 474, 1492, 1666
Hin6I GCGC 3 cut(s) 515, 733, 739
HinP1I GCGC 3 cut(s) 515, 733, 739
HinfI GANTC 8 cut(s) 346, 428, 462, 656, 1148, 1518, 1550, 1590
HpaII CCGG 7 cut(s) 133, 308, 477, 724, 1026, 1062, 1331
HphI GGTGA 6 cut(s) 151, 180, 198, 364, 385, 1369
Hpy166II GTNNAC 2 cut(s) 615, 996
Hpy188I TCNGA 9 cut(s) 173, 661, 897, 1069, 1076, 1228, 1402, 1632, 1664
Hpy188III TCNNGA 8 cut(s) 301, 466, 572, 586, 772, 948, 1008, 1145
Hpy8I GTNNAC 2 cut(s) 615, 996
Hpy99I CGWCG 3 cut(s) 200, 476, 500
HpyAV CCTTC 6 cut(s) 66, 119, 219, 422, 664, 1059
HpyCH4III ACNGT 1 cut(s) 457
HpyCH4IV ACGT 4 cut(s) 203, 388, 635, 1576
HpyF10VI GCNNNNNNNGC 5 cut(s) 161, 950, 1109, 1177, 1450
HpyF3I CTNAG 6 cut(s) 6, 84, 444, 1006, 1068, 1342
HpySE526I ACGT 4 cut(s) 203, 388, 635, 1576
Hsp92I GRCGYC 4 cut(s) 276, 474, 1492, 1666
HspAI GCGC 3 cut(s) 515, 733, 739
KroI GCCGGC 1 cut(s) 132
KroNI GCCGGC 1 cut(s) 134
LmnI GCTCC 7 cut(s) 526, 550, 625, 928, 950, 1333, 1452
Lsp1109I GCAGC 4 cut(s) 176, 928, 1431, 1631
LweI GCATC 4 cut(s) 898, 1139, 1167, 1549
MaeI CTAG 4 cut(s) 69, 122, 836, 1542
MaeII ACGT 4 cut(s) 203, 388, 635, 1576
MaeIII GTNAC 5 cut(s) 204, 391, 599, 1375, 1497
MfeI CAATTG 1 cut(s) 1614
MflI RGATCY 2 cut(s) 967, 1012
MhlI GDGCHC 3 cut(s) 531, 654, 1423
MlsI TGGCCA 1 cut(s) 422
MluCI AATT 4 cut(s) 1077, 1545, 1614, 1640
MluNI TGGCCA 1 cut(s) 422
MlyI GAGTC 4 cut(s) 355, 437, 471, 1142
MmeI TCCRAC 1 cut(s) 439
Mox20I TGGCCA 1 cut(s) 422
MroNI GCCGGC 1 cut(s) 132
MroXI GAANNNNTTC 1 cut(s) 1549
MscI TGGCCA 1 cut(s) 422
MseI TTAA 4 cut(s) 324, 870, 984, 1251
MslI CAYNNNNRTG 4 cut(s) 98, 606, 807, 1458
Msp20I TGGCCA 1 cut(s) 422
MspCI CTTAAG 1 cut(s) 323
MspI CCGG 7 cut(s) 133, 308, 477, 724, 1026, 1062, 1331
MspR9I CCNGG 3 cut(s) 308, 478, 524
MunI CAATTG 1 cut(s) 1614
Mva1269I GAATGC 2 cut(s) 613, 818
MvaI CCWGG 1 cut(s) 524
MvnI CGCG 3 cut(s) 440, 735, 741
MwoI GCNNNNNNNGC 5 cut(s) 161, 950, 1109, 1177, 1450
NaeI GCCGGC 1 cut(s) 134
NciI CCSGG 2 cut(s) 308, 478
NcoI CCATGG 2 cut(s) 93, 1623
NdeI CATATG 1 cut(s) 1507
NgoMIV GCCGGC 1 cut(s) 132
NlaIV GGNNCC 4 cut(s) 137, 312, 320, 1629
NmeAIII GCCGAG 1 cut(s) 295
NmuCI GTSAC 4 cut(s) 204, 391, 599, 1375
NsbI TGCGCA 1 cut(s) 516
NspV TTCGAA 1 cut(s) 489
PaeR7I CTCGAG 1 cut(s) 299
PceI AGGCCT 1 cut(s) 76
PcsI WCGNNNNNNNCGW 3 cut(s) 341, 437, 495
PctI GAATGC 2 cut(s) 613, 818
PdiI GCCGGC 1 cut(s) 134
PdmI GAANNNNTTC 1 cut(s) 1549
PfeI GAWTC 4 cut(s) 656, 1518, 1550, 1590
PfoI TCCNGGA 1 cut(s) 522
PinAI ACCGGT 1 cut(s) 1061
PkrI GCNGC 6 cut(s) 131, 166, 943, 1446, 1621, 1670
Ple19I CGATCG 1 cut(s) 262
PleI GAGTC 4 cut(s) 354, 436, 470, 1142
PmaCI CACGTG 1 cut(s) 389
PmlI CACGTG 1 cut(s) 389
PpsI GAGTC 4 cut(s) 354, 436, 470, 1142
Ppu21I YACGTR 2 cut(s) 389, 636
PshAI GACNNNNGTC 2 cut(s) 148, 389
PshBI ATTAAT 1 cut(s) 1251
Psp6I CCWGG 1 cut(s) 522
PspCI CACGTG 1 cut(s) 389
PspEI GGTNACC 1 cut(s) 1375
PspFI CCCAGC 1 cut(s) 1180
PspGI CCWGG 1 cut(s) 522
PspN4I GGNNCC 4 cut(s) 137, 312, 320, 1629
PspPI GGNCC 8 cut(s) 135, 310, 355, 562, 673, 721, 854, 1628
PstNI CAGNNNCTG 1 cut(s) 1406
PsuI RGATCY 2 cut(s) 967, 1012
PvuI CGATCG 1 cut(s) 262
RsaI GTAC 4 cut(s) 329, 614, 638, 827
RsaNI GTAC 4 cut(s) 328, 613, 637, 826
RseI CAYNNNNRTG 4 cut(s) 98, 606, 807, 1458
SaqAI TTAA 4 cut(s) 324, 870, 984, 1251
SatI GCNGC 6 cut(s) 130, 165, 942, 1445, 1620, 1669
Sau96I GGNCC 8 cut(s) 135, 310, 355, 562, 673, 721, 854, 1628
ScaI AGTACT 1 cut(s) 827
SchI GAGTC 4 cut(s) 355, 437, 471, 1142
ScrFI CCNGG 3 cut(s) 308, 478, 524
SduI GDGCHC 3 cut(s) 531, 654, 1423
SfaNI GCATC 4 cut(s) 898, 1139, 1167, 1549
Sfr274I CTCGAG 1 cut(s) 299
SfuI TTCGAA 1 cut(s) 489
SinI GGWCC 5 cut(s) 310, 355, 721, 854, 1628
SlaI CTCGAG 1 cut(s) 299
SmiMI CAYNNNNRTG 4 cut(s) 98, 606, 807, 1458
SmlI CTYRAG 2 cut(s) 299, 323
SmoI CTYRAG 2 cut(s) 299, 323
Sse9I AATT 4 cut(s) 1077, 1545, 1614, 1640
SseBI AGGCCT 1 cut(s) 76
SspMI CTAG 4 cut(s) 69, 122, 836, 1542
StuI AGGCCT 1 cut(s) 76
StyD4I CCNGG 3 cut(s) 306, 476, 522
StyI CCWWGG 3 cut(s) 93, 645, 1623
TaaI ACNGT 1 cut(s) 457
TaiI ACGT 4 cut(s) 206, 391, 638, 1579
TasI AATT 4 cut(s) 1077, 1545, 1614, 1640
TatI WGTACW 1 cut(s) 825
TauI GCSGC 2 cut(s) 132, 1671
TfiI GAWTC 4 cut(s) 656, 1518, 1550, 1590
Tru1I TTAA 4 cut(s) 324, 870, 984, 1251
Tru9I TTAA 4 cut(s) 324, 870, 984, 1251
TscAI CASTG 5 cut(s) 460, 517, 925, 1027, 1129
TseFI GTSAC 4 cut(s) 204, 391, 599, 1375
TseI GCWGC 4 cut(s) 164, 941, 1444, 1619
Tsp45I GTSAC 4 cut(s) 204, 391, 599, 1375
TspDTI ATGAA 7 cut(s) 116, 201, 797, 978, 1041, 1176, 1582
TspGWI ACGGA 2 cut(s) 368, 563
TspRI CASTG 5 cut(s) 460, 517, 925, 1027, 1129
Vha464I CTTAAG 1 cut(s) 323
VpaK11BI GGWCC 5 cut(s) 310, 355, 721, 854, 1628
VspI ATTAAT 1 cut(s) 1251
XhoI CTCGAG 1 cut(s) 299
XmnI GAANNNNTTC 1 cut(s) 1549
XspI CTAG 4 cut(s) 69, 122, 836, 1542
ZrmI AGTACT 1 cut(s) 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.