pycom17g07420

transmembrane ascorbate ferrireductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0017157
Physical Location & Seq
Forward (+)
5339360 .. 5341183
1824 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 702 bp
ATGGCGGTTCCGGTGGTCCCGTTCCCGATCTTGAATGCAGTGAGGGTTATAGGAGTGATAGTGGCGGCTCTGGTGCTCACGTGGAACGTTCATTTCCGCGGTGGATTGGCTCTCATTTCCGATGACAAAAGTCTTATTTTCAATGTTCATCCGGTATTAATGGTGATTGGGTTGATACTGCTCAATGGAGAAGCCATGCTAGCATACAAGACGGTTTCAGGAACAAAAAACTTGAAAAAATTAGTTCATCTTACACTACAATTCCTTGCCTTTTGCTTAAGCATCATAGGCGTATGGGCTGCTTTGAAGTTCCACAACGATAAGGGCATTGACAACTTCTACAGTCTGCATTCCTGGTTGGGTCTAGCTTGCTTATTCCTCTTCACCCTCCAGTGGGCTGCTGGATTTGTAACGTATTGGTTCCCAGGAGGCTCGAAACACAGCAGAGCTAACTTGCTGCCATGGCATGTATTTTTCGGTGTTTATACTTACGCCCTTGCTGTTGTTACAGTAACTACTGGTATTTTAGAAAAAGTCACCTTCCTCCAAACCCACCAAGTAATCTCACGCTATTCAACCGAGGCTTTGCTCGTGAACTCTCTCGGAATATTGGTCGTTGTTCTGGGAGGTTTTGTTATCCTTGCCGTTATTACTCCGATGAATAATAATAGAGGTGATGTTCTCAGAGGACCAATAGAGTAA

Protein Analysis

234

Amino Acids

25.59

Weight (kDa)

9.48

Isoelectric Point (pI)

27.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cytochrom_B561 PF03188 49 - 181 2.4e-48 Eukaryotic cytochrome b561
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017157)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G38630
fragaria_vesca FvH4_6g45440 FvH4_6g45440 FvH4_6g45440
malus_domestica MD17G1073300.v1.1
prunus_persica Prupe.3G240600_v2.0.a1
pyrus_communis pycom17g07420
rosa_chinensis RchiOBHm_Chr2g0163271
rosa_laevigata RLG00000021387
rosa_roxburghii Rroxscaffold_2G00087420
rosa_rugosa Rorug02G0501900
rosa_samantha Rh2BG580800
rosa_wichuraiana Rw2G047050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 99
AciI CCGC 4 cut(s) 5, 65, 97, 99
AclI AACGTT 1 cut(s) 87
AcvI CACGTG 1 cut(s) 81
AfiI CCNNNNNNNGG 2 cut(s) 393, 394
AflII CTTAAG 1 cut(s) 277
AgsI TTSAA 5 cut(s) 34, 142, 235, 307, 576
AjnI CCWGG 2 cut(s) 353, 424
AluBI AGCT 2 cut(s) 368, 449
AluI AGCT 2 cut(s) 368, 449
Alw21I GWGCWC 1 cut(s) 78
ApeKI GCWGC 3 cut(s) 299, 398, 457
AseI ATTAAT 1 cut(s) 158
AspS9I GGNCC 2 cut(s) 16, 689
AsuHPI GGTGA 4 cut(s) 175, 376, 529, 686
AsuNHI GCTAGC 1 cut(s) 199
AvaII GGWCC 2 cut(s) 16, 689
BauI CACGAG 1 cut(s) 590
BbrPI CACGTG 1 cut(s) 81
Bbv12I GWGCWC 1 cut(s) 78
BbvI GCAGC 3 cut(s) 286, 385, 444
BceAI ACGGC 1 cut(s) 629
BciT130I CCWGG 2 cut(s) 355, 426
BfaI CTAG 2 cut(s) 200, 365
BfmI CTRYAG 1 cut(s) 340
BfrI CTTAAG 1 cut(s) 277
BisI GCNGC 4 cut(s) 66, 300, 399, 458
BlsI GCNGC 4 cut(s) 67, 301, 400, 459
Bme1390I CCNGG 2 cut(s) 355, 426
Bme18I GGWCC 2 cut(s) 16, 689
BmgT120I GGNCC 2 cut(s) 16, 689
BmiI GGNNCC 3 cut(s) 9, 18, 422
BmrFI CCNGG 2 cut(s) 355, 426
BmsI GCATC 1 cut(s) 291
BmtI GCTAGC 1 cut(s) 203
BoxI GACNNNNGTC 1 cut(s) 129
BpmI CTGGAG 1 cut(s) 374
BsaAI YACGTR 1 cut(s) 81
BsaJI CCNNGG 4 cut(s) 97, 424, 461, 579
BsaWI WCCGGW 2 cut(s) 10, 151
BsaXI ACNNNNNCTCC 4 cut(s) 45, 75, 618, 648
Bsc4I CCNNNNNNNGG 2 cut(s) 393, 394
Bse1I ACTGG 2 cut(s) 391, 523
BseBI CCWGG 2 cut(s) 355, 426
BseDI CCNNGG 4 cut(s) 97, 424, 461, 579
BseGI GGATG 1 cut(s) 148
BseLI CCNNNNNNNGG 2 cut(s) 393, 394
BseMII CTCAG 1 cut(s) 697
BseNI ACTGG 2 cut(s) 391, 523
BseXI GCAGC 3 cut(s) 286, 385, 444
Bsh1236I CGCG 1 cut(s) 99
BsiHKAI GWGCWC 1 cut(s) 78
BsiSI CCGG 2 cut(s) 11, 152
BslFI GGGAC 1 cut(s) 2
BslI CCNNNNNNNGG 2 cut(s) 393, 394
BsmFI GGGAC 1 cut(s) 2
BsmI GAATGC 2 cut(s) 40, 349
Bsp1286I GDGCHC 1 cut(s) 78
Bsp143I GATC 1 cut(s) 27
Bsp19I CCATGG 1 cut(s) 461
BspACI CCGC 4 cut(s) 5, 65, 97, 99
BspCNI CTCAG 1 cut(s) 696
BspFNI CGCG 1 cut(s) 99
BspLI GGNNCC 3 cut(s) 9, 18, 422
BspOI GCTAGC 1 cut(s) 203
BspTI CTTAAG 1 cut(s) 277
BsrI ACTGG 2 cut(s) 391, 523
BssECI CCNNGG 4 cut(s) 97, 424, 461, 579
BssMI GATC 1 cut(s) 27
BssSI CACGAG 1 cut(s) 590
BssT1I CCWWGG 1 cut(s) 461
Bst2BI CACGAG 1 cut(s) 590
Bst2UI CCWGG 2 cut(s) 355, 426
Bst4CI ACNGT 3 cut(s) 214, 344, 511
Bst6I CTCTTC 1 cut(s) 386
BstAFI CTTAAG 1 cut(s) 277
BstBAI YACGTR 1 cut(s) 81
BstC8I GCNNGC 2 cut(s) 201, 370
BstDEI CTNAG 1 cut(s) 683
BstDSI CCRYGG 2 cut(s) 97, 461
BstF5I GGATG 1 cut(s) 148
BstFNI CGCG 1 cut(s) 99
BstKTI GATC 1 cut(s) 30
BstMBI GATC 1 cut(s) 27
BstMWI GCNNNNNNNGC 3 cut(s) 200, 288, 463
BstNI CCWGG 2 cut(s) 355, 426
BstNSI RCATGY 1 cut(s) 470
BstPAI GACNNNNGTC 1 cut(s) 129
BstSCI CCNGG 2 cut(s) 353, 424
BstSFI CTRYAG 1 cut(s) 340
BstUI CGCG 1 cut(s) 99
BstV1I GCAGC 3 cut(s) 286, 385, 444
BtgI CCRYGG 2 cut(s) 97, 461
BtsCI GGATG 1 cut(s) 148
BtsI GCAGTG 1 cut(s) 45
BtsIMutI CAGTG 2 cut(s) 45, 398
Cac8I GCNNGC 2 cut(s) 201, 370
Cfr13I GGNCC 2 cut(s) 16, 689
Cfr42I CCGCGG 1 cut(s) 100
CviAII CATG 3 cut(s) 196, 462, 467
CviJI RGCY 9 cut(s) 68, 110, 194, 299, 368, 398, 432, 449, 584
CviKI_1 RGCY 9 cut(s) 68, 110, 194, 299, 368, 398, 432, 449, 584
DdeI CTNAG 1 cut(s) 683
DpnI GATC 1 cut(s) 29
DpnII GATC 1 cut(s) 27
Eam1104I CTCTTC 1 cut(s) 386
EarI CTCTTC 1 cut(s) 386
Eco130I CCWWGG 1 cut(s) 461
Eco47I GGWCC 2 cut(s) 16, 689
Eco72I CACGTG 1 cut(s) 81
EcoRII CCWGG 2 cut(s) 353, 424
EcoT14I CCWWGG 1 cut(s) 461
ErhI CCWWGG 1 cut(s) 461
FaeI CATG 3 cut(s) 199, 465, 470
FaiI YATR 8 cut(s) 50, 197, 205, 287, 295, 463, 468, 486
FaqI GGGAC 1 cut(s) 2
FatI CATG 3 cut(s) 195, 461, 466
Fnu4HI GCNGC 4 cut(s) 66, 300, 399, 458
FokI GGATG 1 cut(s) 135
Fsp4HI GCNGC 4 cut(s) 66, 300, 399, 458
FspBI CTAG 2 cut(s) 200, 365
GluI GCNGC 4 cut(s) 66, 300, 399, 458
GsuI CTGGAG 1 cut(s) 374
HapII CCGG 2 cut(s) 11, 152
Hin1II CATG 3 cut(s) 199, 465, 470
HpaII CCGG 2 cut(s) 11, 152
HphI GGTGA 4 cut(s) 175, 376, 529, 686
Hpy166II GTNNAC 1 cut(s) 595
Hpy188I TCNGA 4 cut(s) 121, 605, 657, 686
Hpy188III TCNNGA 4 cut(s) 25, 31, 219, 592
Hpy8I GTNNAC 1 cut(s) 595
HpyAV CCTTC 1 cut(s) 550
HpyCH4III ACNGT 3 cut(s) 214, 344, 511
HpyCH4IV ACGT 3 cut(s) 80, 87, 413
HpyCH4V TGCA 2 cut(s) 38, 349
HpyF10VI GCNNNNNNNGC 3 cut(s) 200, 288, 463
HpyF3I CTNAG 1 cut(s) 683
HpySE526I ACGT 3 cut(s) 80, 87, 413
Hsp92II CATG 3 cut(s) 199, 465, 470
KspI CCGCGG 1 cut(s) 100
Kzo9I GATC 1 cut(s) 27
Lsp1109I GCAGC 3 cut(s) 286, 385, 444
LweI GCATC 1 cut(s) 291
MaeI CTAG 2 cut(s) 200, 365
MaeII ACGT 3 cut(s) 80, 87, 413
MaeIII GTNAC 4 cut(s) 409, 505, 511, 535
MalI GATC 1 cut(s) 29
MboI GATC 1 cut(s) 27
MboII GAAGA 1 cut(s) 373
MhlI GDGCHC 1 cut(s) 78
MluCI AATT 2 cut(s) 239, 260
MnlI CCTC 9 cut(s) 36, 389, 398, 422, 554, 574, 620, 665, 680
MseI TTAA 2 cut(s) 158, 278
MspA1I CMGCKG 1 cut(s) 99
MspCI CTTAAG 1 cut(s) 277
MspI CCGG 2 cut(s) 11, 152
MspR9I CCNGG 2 cut(s) 355, 426
Mva1269I GAATGC 2 cut(s) 40, 349
MvaI CCWGG 2 cut(s) 355, 426
MvnI CGCG 1 cut(s) 99
MwoI GCNNNNNNNGC 3 cut(s) 200, 288, 463
NcoI CCATGG 1 cut(s) 461
NdeII GATC 1 cut(s) 27
NheI GCTAGC 1 cut(s) 199
NlaIII CATG 3 cut(s) 199, 465, 470
NlaIV GGNNCC 3 cut(s) 9, 18, 422
NmuCI GTSAC 1 cut(s) 535
NspI RCATGY 1 cut(s) 470
PctI GAATGC 2 cut(s) 40, 349
PkrI GCNGC 4 cut(s) 67, 301, 400, 459
PmaCI CACGTG 1 cut(s) 81
PmlI CACGTG 1 cut(s) 81
Ppu21I YACGTR 1 cut(s) 81
PshAI GACNNNNGTC 1 cut(s) 129
PshBI ATTAAT 1 cut(s) 158
Psp1406I AACGTT 1 cut(s) 87
Psp6I CCWGG 2 cut(s) 353, 424
PspCI CACGTG 1 cut(s) 81
PspGI CCWGG 2 cut(s) 353, 424
PspN4I GGNNCC 3 cut(s) 9, 18, 422
PspPI GGNCC 2 cut(s) 16, 689
SacII CCGCGG 1 cut(s) 100
SaqAI TTAA 2 cut(s) 158, 278
SatI GCNGC 4 cut(s) 66, 300, 399, 458
Sau3AI GATC 1 cut(s) 27
Sau96I GGNCC 2 cut(s) 16, 689
ScrFI CCNGG 2 cut(s) 355, 426
SduI GDGCHC 1 cut(s) 78
SetI ASST 8 cut(s) 83, 90, 370, 416, 451, 542, 631, 676
SfaNI GCATC 1 cut(s) 291
SfcI CTRYAG 1 cut(s) 340
Sfr303I CCGCGG 1 cut(s) 100
SgrBI CCGCGG 1 cut(s) 100
SinI GGWCC 2 cut(s) 16, 689
SmlI CTYRAG 1 cut(s) 277
SmoI CTYRAG 1 cut(s) 277
Sse9I AATT 2 cut(s) 239, 260
SsiI CCGC 4 cut(s) 5, 65, 97, 99
SspI AATATT 1 cut(s) 609
SspMI CTAG 2 cut(s) 200, 365
StyD4I CCNGG 2 cut(s) 353, 424
StyI CCWWGG 1 cut(s) 461
TaaI ACNGT 3 cut(s) 214, 344, 511
TaiI ACGT 3 cut(s) 83, 90, 416
TaqI TCGA 1 cut(s) 434
TasI AATT 2 cut(s) 239, 260
TauI GCSGC 1 cut(s) 68
Tru1I TTAA 2 cut(s) 158, 278
Tru9I TTAA 2 cut(s) 158, 278
TscAI CASTG 2 cut(s) 45, 398
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 3 cut(s) 299, 398, 457
Tsp45I GTSAC 1 cut(s) 535
TspDTI ATGAA 4 cut(s) 80, 137, 236, 674
TspRI CASTG 2 cut(s) 45, 398
Vha464I CTTAAG 1 cut(s) 277
VpaK11BI GGWCC 2 cut(s) 16, 689
VspI ATTAAT 1 cut(s) 158
XceI RCATGY 1 cut(s) 470
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XspI CTAG 2 cut(s) 200, 365
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.