pycom17g07670

30S ribosomal protein 3

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012376
Physical Location & Seq
Forward (+)
5589237 .. 5590392
1156 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGTTATCTGTGGGAATGGGAATAGAGTGCAAGGCAAACCTGAACGCCGCCGTATTGCGGCTGAAGCTGCCGCAGCAAAAGCTTCTATTTGTCAACAGTGTTAATCAACGAGCATTGAATTTGAAGCCGAAGACTTGTTCTTTCTCTGTTTCTCACTCGCTGCCAGAGCTGAGGCTCGGACATACTCGTGCCGCAAGAAACGTCAGGCTCTCAGCCGCCGCCGAGTCTCTGCTCGCCGAAGAAACGGCCACCGATGACGACAACACCGCCCCAGAAAAAGAGAAGCTGGGAGTGGTGGTGAAGCCATTTGAGAAACCGAGGCTCGTATTGAAGTTTATTTGGATGGAAAAGAACATCGGCATTGCGCTGGACCAGATGATACCCGGCCACGGCTCCATCCCTCTCAGCCCCTATTACTTCTGGCCGAGGAAAGATGCGTGGGAGGAGCTCAAGGTCTTGCTCGAAAGCAAGCCCTGGATATCTCAGAAGCAGATGATTATCCTTCTCAATCAGGCTACCGATATCATCAATTTGTGGCAGCAGAGCGGCGGCAACCTGGCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PSRP-3_Ycf65

Protein Analysis

188

Amino Acids

20.95

Weight (kDa)

9.27

Isoelectric Point (pI)

35.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PSRP-3_Ycf65 PF04839 135 - 181 2.1e-26 Plastid and cyanobacterial ribosomal protein (PSRP-3 / Ycf65)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012376)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G68590 AT1G68590
fragaria_vesca FvH4_6g45210
malus_domestica MD09G1087300.v1.1 MD17G1075600.v1.1 MD17G1076100.v1.1
prunus_persica Prupe.3G238100_v2.0.a1
pyrus_communis pycom09g01270 pycom17g07670
rosa_chinensis RchiOBHm_Chr2g0162921
rosa_laevigata RLG00000021361
rosa_multiflora Rmu_sc0006129.1_g000003
rosa_roxburghii Rroxscaffold_2G00087710
rosa_rugosa Rorug02G0499300
rosa_samantha Rh2AG565300 Rh2BG578000 Rh2CG547700 Rh2DG588200
rosa_wichuraiana Rw2G046790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 546
AciI CCGC 9 cut(s) 48, 58, 71, 192, 216, 219, 267, 546, 549
AcoI YGGCCR 3 cut(s) 246, 385, 422
AcsI RAATTY 1 cut(s) 118
AcuI CTGAAG 1 cut(s) 83
AfiI CCNNNNNNNGG 2 cut(s) 57, 389
AgsI TTSAA 3 cut(s) 118, 124, 331
AjnI CCWGG 2 cut(s) 473, 555
AluBI AGCT 5 cut(s) 67, 82, 169, 286, 448
AluI AGCT 5 cut(s) 67, 82, 169, 286, 448
Alw21I GWGCWC 1 cut(s) 450
Alw26I GTCTC 1 cut(s) 231
AoxI GGCC 3 cut(s) 246, 385, 422
ApeKI GCWGC 4 cut(s) 67, 73, 160, 538
ApoI RAATTY 1 cut(s) 118
AspLEI GCGC 1 cut(s) 367
AspS9I GGNCC 1 cut(s) 370
AsuC2I CCSGG 1 cut(s) 384
AsuHPI GGTGA 1 cut(s) 310
AvaII GGWCC 1 cut(s) 370
BanII GRGCYC 1 cut(s) 450
BauI CACGAG 1 cut(s) 186
BbsI GAAGAC 1 cut(s) 137
Bbv12I GWGCWC 1 cut(s) 450
BbvCI CCTCAGC 1 cut(s) 170
BbvI GCAGC 4 cut(s) 54, 85, 147, 550
BccI CCATC 2 cut(s) 337, 404
BceAI ACGGC 3 cut(s) 35, 261, 406
BciT130I CCWGG 2 cut(s) 475, 557
BcnI CCSGG 1 cut(s) 384
BcoDI GTCTC 1 cut(s) 231
Bme1390I CCNGG 3 cut(s) 384, 475, 557
Bme18I GGWCC 1 cut(s) 370
BmgT120I GGNCC 1 cut(s) 370
BmiI GGNNCC 1 cut(s) 394
BmrFI CCNGG 3 cut(s) 384, 475, 557
BmsI GCATC 1 cut(s) 424
BpiI GAAGAC 1 cut(s) 137
Bpu10I CCTNAGC 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 434
BpuMI CCSGG 1 cut(s) 384
BsaBI GATNNNNATC 1 cut(s) 497
BsaJI CCNNGG 4 cut(s) 317, 388, 425, 473
BsaXI ACNNNNNCTCC 2 cut(s) 282, 312
Bsc4I CCNNNNNNNGG 2 cut(s) 57, 389
Bse3DI GCAATG 1 cut(s) 360
Bse8I GATNNNNATC 1 cut(s) 497
BseBI CCWGG 2 cut(s) 475, 557
BseDI CCNNGG 4 cut(s) 317, 388, 425, 473
BseGI GGATG 2 cut(s) 348, 396
BseJI GATNNNNATC 1 cut(s) 497
BseLI CCNNNNNNNGG 2 cut(s) 57, 389
BseMI GCAATG 1 cut(s) 360
BseMII CTCAG 4 cut(s) 161, 225, 418, 497
BseRI GAGGAG 1 cut(s) 458
BseXI GCAGC 4 cut(s) 54, 85, 147, 550
BseYI CCCAGC 1 cut(s) 286
BshFI GGCC 3 cut(s) 248, 387, 424
BsiHKAI GWGCWC 1 cut(s) 450
BsiSI CCGG 1 cut(s) 384
BslI CCNNNNNNNGG 2 cut(s) 57, 389
BsmAI GTCTC 1 cut(s) 231
BsnI GGCC 3 cut(s) 248, 387, 424
Bsp1286I GDGCHC 1 cut(s) 450
BspACI CCGC 9 cut(s) 48, 58, 71, 192, 216, 219, 267, 546, 549
BspANI GGCC 3 cut(s) 248, 387, 424
BspCNI CTCAG 4 cut(s) 162, 224, 417, 496
BspLI GGNNCC 1 cut(s) 394
BsrBI CCGCTC 1 cut(s) 546
BsrDI GCAATG 1 cut(s) 360
BssECI CCNNGG 4 cut(s) 317, 388, 425, 473
BssSI CACGAG 1 cut(s) 186
Bst2BI CACGAG 1 cut(s) 186
Bst2UI CCWGG 2 cut(s) 475, 557
Bst4CI ACNGT 1 cut(s) 98
BstC8I GCNNGC 2 cut(s) 234, 470
BstDEI CTNAG 4 cut(s) 170, 211, 404, 483
BstDSI CCRYGG 1 cut(s) 388
BstF5I GGATG 2 cut(s) 348, 396
BstHHI GCGC 1 cut(s) 367
BstMAI GTCTC 1 cut(s) 231
BstMWI GCNNNNNNNGC 5 cut(s) 64, 67, 73, 79, 166
BstNI CCWGG 2 cut(s) 475, 557
BstSCI CCNGG 3 cut(s) 382, 473, 555
BstV1I GCAGC 4 cut(s) 54, 85, 147, 550
BstV2I GAAGAC 1 cut(s) 137
BsuRI GGCC 3 cut(s) 248, 387, 424
BtgI CCRYGG 1 cut(s) 388
BtsCI GGATG 2 cut(s) 348, 396
BtsIMutI CAGTG 1 cut(s) 103
Cac8I GCNNGC 2 cut(s) 234, 470
CfoI GCGC 1 cut(s) 367
Cfr13I GGNCC 1 cut(s) 370
DdeI CTNAG 4 cut(s) 170, 211, 404, 483
EaeI YGGCCR 3 cut(s) 246, 385, 422
Ecl136II GAGCTC 1 cut(s) 448
Eco24I GRGCYC 1 cut(s) 450
Eco32I GATATC 2 cut(s) 480, 523
Eco47I GGWCC 1 cut(s) 370
Eco53kI GAGCTC 1 cut(s) 448
Eco57I CTGAAG 1 cut(s) 83
EcoICRI GAGCTC 1 cut(s) 448
EcoRII CCWGG 2 cut(s) 473, 555
EcoRV GATATC 2 cut(s) 480, 523
EcoT38I GRGCYC 1 cut(s) 450
FaiI YATR 1 cut(s) 183
FokI GGATG 2 cut(s) 355, 383
FriOI GRGCYC 1 cut(s) 450
GlaI GCGC 1 cut(s) 366
GsaI CCCAGC 1 cut(s) 290
HaeIII GGCC 3 cut(s) 248, 387, 424
HapII CCGG 1 cut(s) 384
HhaI GCGC 1 cut(s) 367
Hin6I GCGC 1 cut(s) 365
HinP1I GCGC 1 cut(s) 365
HincII GTYRAC 1 cut(s) 94
HindII GTYRAC 1 cut(s) 94
HindIII AAGCTT 1 cut(s) 80
HinfI GANTC 1 cut(s) 224
HpaII CCGG 1 cut(s) 384
HphI GGTGA 1 cut(s) 310
Hpy166II GTNNAC 1 cut(s) 94
Hpy188I TCNGA 2 cut(s) 179, 486
Hpy8I GTNNAC 1 cut(s) 94
HpyAV CCTTC 1 cut(s) 512
HpyCH4III ACNGT 1 cut(s) 98
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 1 cut(s) 30
HpyF10VI GCNNNNNNNGC 5 cut(s) 64, 67, 73, 79, 166
HpyF3I CTNAG 4 cut(s) 170, 211, 404, 483
HpySE526I ACGT 1 cut(s) 201
HspAI GCGC 1 cut(s) 365
LmnI GCTCC 2 cut(s) 398, 445
Lsp1109I GCAGC 4 cut(s) 54, 85, 147, 550
LweI GCATC 1 cut(s) 424
MaeII ACGT 1 cut(s) 201
MbiI CCGCTC 1 cut(s) 546
MboII GAAGA 2 cut(s) 142, 251
MhlI GDGCHC 1 cut(s) 450
MluCI AATT 2 cut(s) 118, 529
MlyI GAGTC 1 cut(s) 233
MnlI CCTC 5 cut(s) 165, 312, 411, 420, 436
MseI TTAA 1 cut(s) 102
MslI CAYNNNNRTG 1 cut(s) 186
MspI CCGG 1 cut(s) 384
MspR9I CCNGG 3 cut(s) 384, 475, 557
MvaI CCWGG 2 cut(s) 475, 557
MwoI GCNNNNNNNGC 5 cut(s) 64, 67, 73, 79, 166
NciI CCSGG 1 cut(s) 384
NlaIV GGNNCC 1 cut(s) 394
NmeAIII GCCGAG 2 cut(s) 247, 450
PleI GAGTC 1 cut(s) 232
PpsI GAGTC 1 cut(s) 232
Psp124BI GAGCTC 1 cut(s) 450
Psp6I CCWGG 2 cut(s) 473, 555
PspFI CCCAGC 1 cut(s) 286
PspGI CCWGG 2 cut(s) 473, 555
PspN4I GGNNCC 1 cut(s) 394
PspPI GGNCC 1 cut(s) 370
RseI CAYNNNNRTG 1 cut(s) 186
SacI GAGCTC 1 cut(s) 450
SaqAI TTAA 1 cut(s) 102
Sau96I GGNCC 1 cut(s) 370
SchI GAGTC 1 cut(s) 233
ScrFI CCNGG 3 cut(s) 384, 475, 557
SduI GDGCHC 1 cut(s) 450
SetI ASST 9 cut(s) 42, 69, 84, 171, 204, 288, 450, 456, 558
SfaNI GCATC 1 cut(s) 424
SinI GGWCC 1 cut(s) 370
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 449
SmoI CTYRAG 1 cut(s) 449
Sse9I AATT 2 cut(s) 118, 529
SsiI CCGC 9 cut(s) 48, 58, 71, 192, 216, 219, 267, 546, 549
SstI GAGCTC 1 cut(s) 450
StyD4I CCNGG 3 cut(s) 382, 473, 555
TaaI ACNGT 1 cut(s) 98
TaiI ACGT 1 cut(s) 204
TaqI TCGA 1 cut(s) 462
TasI AATT 2 cut(s) 118, 529
TauI GCSGC 8 cut(s) 50, 61, 73, 194, 218, 221, 549, 552
Tru1I TTAA 1 cut(s) 102
Tru9I TTAA 1 cut(s) 102
TscAI CASTG 1 cut(s) 103
TseI GCWGC 4 cut(s) 67, 73, 160, 538
TspRI CASTG 1 cut(s) 103
VpaK11BI GGWCC 1 cut(s) 370
XapI RAATTY 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.