pycom17g12210

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0010197
Physical Location & Seq
Reverse (-)
9956480 .. 9957037
558 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 558 bp
ATGACTAGATACATCATAGTGCATGTCAGGCACTTTTTTTCTTGGTTAAGAGGGGGAAAAGGTTACAAAATGAAAGTTAAACGTTCAGTTAAAAGCGCACAAACAGCCTTCTCTGCTCTTCCTCTACCTCTCTCCACACCCACCCCCACCCCCACCTCTCAAACCACCACACCCACCAGCCCAGACCACCTCTCCTCCCCAATCATCTACCTCCCAAAGTTCAAAACCCCCACCGTCCGCTCCCGTATCAGCAAACTCTGCCAGGAAGGCCAACTCCACCTTGCCCGCCAACTGTTCGACACTTTGCCTCGCCCCTCCACTATGCTTTGGAACACCATCATCATTGGATTCCTCTACAACAACATGCCCAATGAATCCCTCTTGTTCTACTCCCAGATGAAATCCTCCTTTCCCGGCACCAAGGCCGACCCTTACACTTACTCTTCCACCCTCATAGCTTGCGCCGACACCCGCAATTTTAAGATGGGTAAAGCCATGCATTGCCATGTTCTTCGGTGCCTTCCCAACCCCAGTAGGACTGTGTGCAATTCGCTTTGA

Protein Analysis

186

Amino Acids

20.9

Weight (kDa)

10.15

Isoelectric Point (pI)

51.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 105 - 155 4.4e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 416, 516
AccBSI CCGCTC 1 cut(s) 240
AciI CCGC 3 cut(s) 238, 286, 472
AclI AACGTT 1 cut(s) 82
AgsI TTSAA 1 cut(s) 223
AjnI CCWGG 1 cut(s) 261
AluBI AGCT 1 cut(s) 458
AluI AGCT 1 cut(s) 458
AoxI GGCC 2 cut(s) 268, 423
AspLEI GCGC 2 cut(s) 98, 464
AsuC2I CCSGG 1 cut(s) 414
BanI GGYRCC 2 cut(s) 416, 516
BccI CCATC 2 cut(s) 344, 478
BciT130I CCWGG 1 cut(s) 263
BcnI CCSGG 1 cut(s) 414
BfaI CTAG 1 cut(s) 6
BglI GCCNNNNNGGC 1 cut(s) 267
Bme1390I CCNGG 2 cut(s) 263, 414
BmiI GGNNCC 2 cut(s) 418, 518
BmrFI CCNGG 2 cut(s) 263, 414
BmrI ACTGGG 1 cut(s) 525
BmuI ACTGGG 1 cut(s) 525
BpuMI CCSGG 1 cut(s) 414
BsaJI CCNNGG 1 cut(s) 420
BsaXI ACNNNNNCTCC 4 cut(s) 176, 179, 206, 209
Bse1I ACTGG 1 cut(s) 531
Bse3DI GCAATG 1 cut(s) 499
BseBI CCWGG 1 cut(s) 263
BseDI CCNNGG 1 cut(s) 420
BseMI GCAATG 1 cut(s) 499
BseNI ACTGG 1 cut(s) 531
BseRI GAGGAG 1 cut(s) 184
BshFI GGCC 2 cut(s) 270, 425
BshNI GGYRCC 2 cut(s) 416, 516
BsiSI CCGG 1 cut(s) 414
BsnI GGCC 2 cut(s) 270, 425
BspACI CCGC 3 cut(s) 238, 286, 472
BspANI GGCC 2 cut(s) 270, 425
BspLI GGNNCC 2 cut(s) 418, 518
BspQI GCTCTTC 1 cut(s) 123
BspT107I GGYRCC 2 cut(s) 416, 516
BsrBI CCGCTC 1 cut(s) 240
BsrDI GCAATG 1 cut(s) 499
BsrI ACTGG 1 cut(s) 531
BssECI CCNNGG 1 cut(s) 420
BssT1I CCWWGG 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 263
Bst4CI ACNGT 3 cut(s) 235, 294, 541
Bst6I CTCTTC 2 cut(s) 123, 448
BstAPI GCANNNNNTGC 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 286, 460
BstHHI GCGC 2 cut(s) 98, 464
BstMWI GCNNNNNNNGC 5 cut(s) 28, 104, 113, 258, 267
BstNI CCWGG 1 cut(s) 263
BstNSI RCATGY 2 cut(s) 26, 367
BstSCI CCNGG 2 cut(s) 261, 412
BsuRI GGCC 2 cut(s) 270, 425
Cac8I GCNNGC 2 cut(s) 286, 460
CfoI GCGC 2 cut(s) 98, 464
CviAII CATG 4 cut(s) 23, 364, 496, 506
CviJI RGCY 6 cut(s) 107, 180, 270, 425, 458, 494
CviKI_1 RGCY 6 cut(s) 107, 180, 270, 425, 458, 494
Eam1104I CTCTTC 2 cut(s) 123, 448
EarI CTCTTC 2 cut(s) 123, 448
Eco130I CCWWGG 1 cut(s) 420
EcoRII CCWGG 1 cut(s) 261
EcoT14I CCWWGG 1 cut(s) 420
EcoT22I ATGCAT 1 cut(s) 501
ErhI CCWWGG 1 cut(s) 420
FaeI CATG 4 cut(s) 26, 367, 499, 509
FaiI YATR 7 cut(s) 17, 24, 323, 365, 455, 497, 507
FatI CATG 4 cut(s) 22, 363, 495, 505
FauI CCCGC 2 cut(s) 293, 479
FspBI CTAG 1 cut(s) 6
GlaI GCGC 2 cut(s) 97, 463
HaeIII GGCC 2 cut(s) 270, 425
HapII CCGG 1 cut(s) 414
HhaI GCGC 2 cut(s) 98, 464
Hin1II CATG 4 cut(s) 26, 367, 499, 509
Hin6I GCGC 2 cut(s) 96, 462
HinP1I GCGC 2 cut(s) 96, 462
HinfI GANTC 2 cut(s) 348, 374
HpaII CCGG 1 cut(s) 414
HpyAV CCTTC 3 cut(s) 118, 260, 530
HpyCH4III ACNGT 3 cut(s) 235, 294, 541
HpyCH4IV ACGT 1 cut(s) 82
HpyCH4V TGCA 3 cut(s) 22, 499, 546
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 104, 113, 258, 267
HpySE526I ACGT 1 cut(s) 82
Hsp92II CATG 4 cut(s) 26, 367, 499, 509
HspAI GCGC 2 cut(s) 96, 462
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 1 cut(s) 245
LpnPI CCDG 8 cut(s) 13, 190, 195, 248, 275, 407, 427, 544
MaeI CTAG 1 cut(s) 6
MaeII ACGT 1 cut(s) 82
MaeIII GTNAC 1 cut(s) 62
MbiI CCGCTC 1 cut(s) 240
MboII GAAGA 3 cut(s) 110, 435, 503
MluCI AATT 2 cut(s) 475, 547
Mph1103I ATGCAT 1 cut(s) 501
MseI TTAA 4 cut(s) 47, 78, 90, 480
MslI CAYNNNNRTG 2 cut(s) 17, 504
MspI CCGG 1 cut(s) 414
MspR9I CCNGG 2 cut(s) 263, 414
MvaI CCWGG 1 cut(s) 263
MwoI GCNNNNNNNGC 5 cut(s) 28, 104, 113, 258, 267
NciI CCSGG 1 cut(s) 414
NlaIII CATG 4 cut(s) 26, 367, 499, 509
NlaIV GGNNCC 2 cut(s) 418, 518
NsiI ATGCAT 1 cut(s) 501
NspI RCATGY 2 cut(s) 26, 367
PciSI GCTCTTC 1 cut(s) 123
PfeI GAWTC 2 cut(s) 348, 374
Psp1406I AACGTT 1 cut(s) 82
Psp6I CCWGG 1 cut(s) 261
PspGI CCWGG 1 cut(s) 261
PspN4I GGNNCC 2 cut(s) 418, 518
RseI CAYNNNNRTG 2 cut(s) 17, 504
SapI GCTCTTC 1 cut(s) 123
SaqAI TTAA 4 cut(s) 47, 78, 90, 480
ScrFI CCNGG 2 cut(s) 263, 414
SetI ASST 8 cut(s) 64, 85, 130, 158, 192, 213, 282, 460
SmiMI CAYNNNNRTG 2 cut(s) 17, 504
Sse9I AATT 2 cut(s) 475, 547
SsiI CCGC 3 cut(s) 238, 286, 472
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 2 cut(s) 261, 412
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 3 cut(s) 235, 294, 541
TaiI ACGT 1 cut(s) 85
TaqI TCGA 1 cut(s) 297
TasI AATT 2 cut(s) 475, 547
TfiI GAWTC 2 cut(s) 348, 374
Tru1I TTAA 4 cut(s) 47, 78, 90, 480
Tru9I TTAA 4 cut(s) 47, 78, 90, 480
TspDTI ATGAA 3 cut(s) 86, 387, 413
XceI RCATGY 2 cut(s) 26, 367
XspI CTAG 1 cut(s) 6
Zsp2I ATGCAT 1 cut(s) 501
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.