pycom17g12330

Regulator of chromosome condensation (RCC1) repeat

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008533
Physical Location & Seq
Reverse (-)
10018407 .. 10021513
3107 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1389 bp
ATGAGCGCCCACCTGTCTCTCCGCCACCGCTCTTTCGCATACCTCACTCGCTACCTCTCCTCCTCACCCTTCTCCACCGCCGCCGCCACCTCCAAAATCCCCACCCTTTACGAACCCACTGCCGCCACCACCACCCAAGATGACGACGCCCCCACCACAACCCTCCAACTCCTCTCATGGGGCAGAGGCGCCTCCGGCCAGCTCGGCGGCGGCATCGAAGAAATCCGACTCTACCCAACCCCAGTCGCCAACCTCGTCGTTCCCACCTCATGCTTCACTCTTTCACCATCCCAGGGCCGAATTCAAACCCCCAATTGCTCGAATCAAGAGAGCAATGCCGGCCGCAGCATTGCACAGGTCGGTATTTCTTGTGGGTTGTTCCACTCTTCGCTATTGGTGGATGGCAAGCTTTGGATTTGGGGTAAAGGCGACGGCGGTCGACTTGGTTTTGGACATGAGAATTCTCTGTTTCTGCCCACTTTGAACCCGAATTTGGATTCGGTTCGCTTTGTGGCGCTTGGCGGGTTGCATTCGGTGGCGCTCACCACCAACGGCGAAGTCTATACTTGGGGTTATGGTGGTTTTGGTGCACTTGGACATTCTGTTTATACTAGAGAGTTGTTCCCTAGATTTGTAGAAGGATCATGGAGTGGGAAAATATGTCATATTTCAACTAGTGGGACGCATACTGGTGCCATCACGGAATCAGGAGAACTTTATATTTGGGGTCGAGATGAAGGGGATGGTAGATTGGGACTTGGTCCTAATCGAGGCCCAAATGAGGGTGGTGGACTTAGCATACCGTCCAAAGTAAAAGCATTACCTATGCCAGTGGCTGCCGTTTCTTGCGGAGGGTTTTTCACAATGGCACTGATGGAAGATGGGGAACTTTGGAATTGGGGAGCAAACTCTAACTATGAGCTTGGAAGGGGTGATAAGGTTGGTGGTTGGACACCAAAACCAGTTCCTAGTCTTGTAAGCGTTCGTATCATTCAGATAGAAAGTGGTGGATATCATTCTCTTGCGTTAACCGATGAAGGTAAAGTACTTTCTTGGGGACATGGTGGGCATGGTCAGTTGGGCAACTCTTCTTTACAAAGTCAGAAAACACCCACAGTTGTTGAGTCTTTAACTGATGAGCGTGTTATCTATATTGCTTGCGGGGGTTCATCTTCAGCAGCTATAACAGATAAAGGAAAGTTGTATATGTGGGGATATACCAAAGATTCTCAGTTAGGAGTACCTGGGTTGCCAGAGATCGATCCATCCCCCGTTGAAGTCAAGTTTTTAATGGAGGATGATGGATTGGGAGCCCACAATGTGCTGTCTGTTGCAGTTGGTGCTTCCCATGCCATGTGCTTGGTTTCAAGGGATGAATTGTCCAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RCC1

Protein Analysis

463

Amino Acids

48.83

Weight (kDa)

5.81

Isoelectric Point (pI)

37.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_RLD PF25390 56 - 453 8.5e-78 RCC1-like domain
RCC1 PF00415 134 - 181 1.3e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 168 - 196 9.3e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 184 - 233 8.3e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 236 - 291 6e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 278 - 305 5.7e-07 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 294 - 343 1e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 330 - 359 7.6e-10 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 346 - 395 1.8e-13 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 398 - 454 3e-06 Regulator of chromosome condensation (RCC1) repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 188, 692
AccBSI CCGCTC 1 cut(s) 30
AccI GTMKAC 1 cut(s) 439
AclWI GGATC 2 cut(s) 649, 1256
AcoI YGGCCR 2 cut(s) 196, 340
AcsI RAATTY 3 cut(s) 300, 460, 490
AcuI CTGAAG 1 cut(s) 1158
AcyI GRCGYC 2 cut(s) 147, 189
AdeI CACNNNGTG 1 cut(s) 1321
AfaI GTAC 2 cut(s) 1047, 1242
AfiI CCNNNNNNNGG 6 cut(s) 178, 293, 493, 770, 781, 782
AgsI TTSAA 5 cut(s) 305, 484, 672, 1277, 1368
AhlI ACTAGT 1 cut(s) 674
AjnI CCWGG 2 cut(s) 291, 1243
AjuI GAANNNNNNNTTGG 2 cut(s) 476, 508
AluBI AGCT 4 cut(s) 202, 409, 922, 1181
AluI AGCT 4 cut(s) 202, 409, 922, 1181
Alw21I GWGCWC 1 cut(s) 592
Alw26I GTCTC 1 cut(s) 21
Alw44I GTGCAC 1 cut(s) 588
AlwI GGATC 2 cut(s) 649, 1256
AlwNI CAGNNNCTG 1 cut(s) 836
AoxI GGCC 4 cut(s) 196, 295, 340, 772
ApaLI GTGCAC 1 cut(s) 588
ApeKI GCWGC 3 cut(s) 345, 836, 1178
ApoI RAATTY 3 cut(s) 300, 460, 490
AspLEI GCGC 4 cut(s) 8, 191, 517, 541
AspS9I GGNCC 3 cut(s) 295, 761, 773
AsuHPI GGTGA 4 cut(s) 57, 276, 535, 944
AvaII GGWCC 1 cut(s) 761
BaeGI GKGCMC 1 cut(s) 592
BanI GGYRCC 2 cut(s) 188, 692
BanII GRGCYC 1 cut(s) 1315
BarI GAAGNNNNNNTAC 2 cut(s) 1029, 1061
Bbv12I GWGCWC 1 cut(s) 592
BbvI GCAGC 3 cut(s) 357, 823, 1190
BccI CCATC 8 cut(s) 295, 395, 704, 737, 868, 875, 1273, 1295
BceAI ACGGC 3 cut(s) 448, 568, 824
BcgI CGANNNNNNTGC 2 cut(s) 101, 135
BciT130I CCWGG 2 cut(s) 293, 1245
BcoDI GTCTC 1 cut(s) 21
BcuI ACTAGT 1 cut(s) 674
BfaI CTAG 4 cut(s) 612, 627, 675, 969
BfoI RGCGCY 4 cut(s) 9, 192, 518, 542
BglI GCCNNNNNGGC 1 cut(s) 204
BisI GCNGC 9 cut(s) 81, 84, 123, 208, 211, 343, 346, 837, 1179
BlsI GCNGC 9 cut(s) 82, 85, 124, 209, 212, 344, 347, 838, 1180
BmcAI AGTACT 1 cut(s) 1047
Bme1390I CCNGG 2 cut(s) 293, 1245
Bme18I GGWCC 1 cut(s) 761
BmgT120I GGNCC 3 cut(s) 295, 761, 773
BmiI GGNNCC 3 cut(s) 190, 694, 1312
BmrFI CCNGG 2 cut(s) 293, 1245
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 1 cut(s) 222
BmuI ACTGGG 1 cut(s) 236
BoxI GACNNNNGTC 1 cut(s) 435
Bsa29I ATCGAT 1 cut(s) 1260
BsaHI GRCGYC 2 cut(s) 147, 189
BsaJI CCNNGG 3 cut(s) 291, 292, 1244
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 6 cut(s) 178, 293, 493, 770, 781, 782
Bse118I RCCGGY 1 cut(s) 338
Bse1I ACTGG 4 cut(s) 242, 694, 830, 962
Bse3DI GCAATG 2 cut(s) 340, 348
BseBI CCWGG 2 cut(s) 293, 1245
BseCI ATCGAT 1 cut(s) 1260
BseDI CCNNGG 3 cut(s) 291, 292, 1244
BseGI GGATG 6 cut(s) 287, 406, 748, 1265, 1303, 1378
BseLI CCNNNNNNNGG 6 cut(s) 178, 293, 493, 770, 781, 782
BseMI GCAATG 2 cut(s) 340, 348
BseMII CTCAG 1 cut(s) 1244
BseNI ACTGG 4 cut(s) 242, 694, 830, 962
BseRI GAGGAG 3 cut(s) 49, 52, 161
BseSI GKGCMC 1 cut(s) 592
BseX3I CGGCCG 1 cut(s) 340
BseXI GCAGC 3 cut(s) 357, 823, 1190
Bsh1285I CGRYCG 2 cut(s) 343, 439
BshFI GGCC 4 cut(s) 198, 297, 342, 774
BshNI GGYRCC 2 cut(s) 188, 692
BshVI ATCGAT 1 cut(s) 1260
BsiEI CGRYCG 2 cut(s) 343, 439
BsiHKAI GWGCWC 1 cut(s) 592
BsiSI CCGG 2 cut(s) 195, 339
BslFI GGGAC 3 cut(s) 694, 768, 1071
BslI CCNNNNNNNGG 6 cut(s) 178, 293, 493, 770, 781, 782
BsmAI GTCTC 1 cut(s) 21
BsmFI GGGAC 3 cut(s) 694, 768, 1071
BsmI GAATGC 1 cut(s) 529
BsnI GGCC 4 cut(s) 198, 297, 342, 774
Bsp1286I GDGCHC 2 cut(s) 592, 1315
Bsp143I GATC 3 cut(s) 641, 1257, 1261
BspANI GGCC 4 cut(s) 198, 297, 342, 774
BspCNI CTCAG 1 cut(s) 1243
BspDI ATCGAT 1 cut(s) 1260
BspLI GGNNCC 3 cut(s) 190, 694, 1312
BspPI GGATC 2 cut(s) 649, 1256
BspT107I GGYRCC 2 cut(s) 188, 692
BsrBI CCGCTC 1 cut(s) 30
BsrDI GCAATG 2 cut(s) 340, 348
BsrFI RCCGGY 1 cut(s) 338
BsrI ACTGG 4 cut(s) 242, 694, 830, 962
BssAI RCCGGY 1 cut(s) 338
BssECI CCNNGG 3 cut(s) 291, 292, 1244
BssMI GATC 3 cut(s) 641, 1257, 1261
BssNI GRCGYC 2 cut(s) 147, 189
Bst2UI CCWGG 2 cut(s) 293, 1245
Bst4CI ACNGT 2 cut(s) 804, 1117
Bst6I CTCTTC 2 cut(s) 391, 1093
BstACI GRCGYC 2 cut(s) 147, 189
BstAPI GCANNNNNTGC 1 cut(s) 1340
BstC8I GCNNGC 4 cut(s) 200, 340, 407, 1159
BstDEI CTNAG 2 cut(s) 794, 1230
BstENI CCTNNNNNAGG 1 cut(s) 768
BstF5I GGATG 6 cut(s) 287, 406, 748, 1265, 1303, 1378
BstH2I RGCGCY 4 cut(s) 9, 192, 518, 542
BstHHI GCGC 4 cut(s) 8, 191, 517, 541
BstKTI GATC 3 cut(s) 644, 1260, 1264
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 3 cut(s) 641, 1257, 1261
BstMCI CGRYCG 2 cut(s) 343, 439
BstMWI GCNNNNNNNGC 5 cut(s) 195, 204, 339, 1340, 1349
BstNI CCWGG 2 cut(s) 293, 1245
BstPAI GACNNNNGTC 1 cut(s) 435
BstSCI CCNGG 2 cut(s) 291, 1243
BstSLI GKGCMC 1 cut(s) 592
BstV1I GCAGC 3 cut(s) 357, 823, 1190
BstXI CCANNNNNNTGG 1 cut(s) 1360
BstZI CGGCCG 1 cut(s) 340
Bsu15I ATCGAT 1 cut(s) 1260
BsuRI GGCC 4 cut(s) 198, 297, 342, 774
BsuTUI ATCGAT 1 cut(s) 1260
BtsCI GGATG 6 cut(s) 287, 406, 748, 1265, 1303, 1378
BtsI GCAGTG 1 cut(s) 117
BtsIMutI CAGTG 3 cut(s) 117, 837, 869
Cac8I GCNNGC 4 cut(s) 200, 340, 407, 1159
CaiI CAGNNNCTG 1 cut(s) 836
CfoI GCGC 4 cut(s) 8, 191, 517, 541
Cfr10I RCCGGY 1 cut(s) 338
Cfr13I GGNCC 3 cut(s) 295, 761, 773
ClaI ATCGAT 1 cut(s) 1260
CseI GACGC 2 cut(s) 155, 691
Csp6I GTAC 2 cut(s) 1046, 1241
CspCI CAANNNNNGTGG 2 cut(s) 1102, 1137
CviAII CATG 8 cut(s) 177, 270, 455, 645, 1061, 1070, 1349, 1354
CviQI GTAC 2 cut(s) 1046, 1241
DdeI CTNAG 2 cut(s) 794, 1230
DinI GGCGCC 1 cut(s) 190
DpnI GATC 3 cut(s) 643, 1259, 1263
DpnII GATC 3 cut(s) 641, 1257, 1261
DraIII CACNNNGTG 1 cut(s) 1321
EaeI YGGCCR 2 cut(s) 196, 340
EagI CGGCCG 1 cut(s) 340
Eam1104I CTCTTC 2 cut(s) 391, 1093
EarI CTCTTC 2 cut(s) 391, 1093
EciI GGCGGA 1 cut(s) 11
EclXI CGGCCG 1 cut(s) 340
Eco24I GRGCYC 1 cut(s) 1315
Eco32I GATATC 1 cut(s) 1013
Eco47I GGWCC 1 cut(s) 761
Eco52I CGGCCG 1 cut(s) 340
Eco57I CTGAAG 1 cut(s) 1158
EcoNI CCTNNNNNAGG 1 cut(s) 768
EcoRI GAATTC 2 cut(s) 300, 460
EcoRII CCWGG 2 cut(s) 291, 1243
EcoRV GATATC 1 cut(s) 1013
EcoT38I GRGCYC 1 cut(s) 1315
EgeI GGCGCC 1 cut(s) 190
EheI GGCGCC 1 cut(s) 190
FaeI CATG 8 cut(s) 180, 273, 458, 648, 1064, 1073, 1352, 1357
FaqI GGGAC 3 cut(s) 694, 768, 1071
FatI CATG 8 cut(s) 176, 269, 454, 644, 1060, 1069, 1348, 1353
FauI CCCGC 2 cut(s) 515, 1154
FblI GTMKAC 1 cut(s) 439
Fnu4HI GCNGC 9 cut(s) 81, 84, 123, 208, 211, 343, 346, 837, 1179
FokI GGATG 6 cut(s) 274, 413, 755, 1252, 1310, 1385
FriOI GRGCYC 1 cut(s) 1315
Fsp4HI GCNGC 9 cut(s) 81, 84, 123, 208, 211, 343, 346, 837, 1179
FspBI CTAG 4 cut(s) 612, 627, 675, 969
GlaI GCGC 4 cut(s) 7, 190, 516, 540
GluI GCNGC 9 cut(s) 81, 84, 123, 208, 211, 343, 346, 837, 1179
HaeII RGCGCY 4 cut(s) 9, 192, 518, 542
HaeIII GGCC 4 cut(s) 198, 297, 342, 774
HapII CCGG 2 cut(s) 195, 339
HgaI GACGC 2 cut(s) 155, 691
HhaI GCGC 4 cut(s) 8, 191, 517, 541
Hin1I GRCGYC 2 cut(s) 147, 189
Hin1II CATG 8 cut(s) 180, 273, 458, 648, 1064, 1073, 1352, 1357
Hin6I GCGC 4 cut(s) 6, 189, 515, 539
HinP1I GCGC 4 cut(s) 6, 189, 515, 539
HincII GTYRAC 2 cut(s) 440, 1029
HindII GTYRAC 2 cut(s) 440, 1029
HindIII AAGCTT 1 cut(s) 407
HinfI GANTC 6 cut(s) 228, 322, 497, 704, 1124, 1226
HpaI GTTAAC 1 cut(s) 1029
HpaII CCGG 2 cut(s) 195, 339
HphI GGTGA 4 cut(s) 57, 276, 535, 944
Hpy166II GTNNAC 4 cut(s) 440, 590, 791, 1029
Hpy188I TCNGA 3 cut(s) 227, 996, 1104
Hpy188III TCNNGA 3 cut(s) 326, 708, 731
Hpy8I GTNNAC 4 cut(s) 440, 590, 791, 1029
Hpy99I CGWCG 3 cut(s) 149, 260, 434
HpyAV CCTTC 5 cut(s) 79, 632, 731, 921, 1031
HpyCH4III ACNGT 2 cut(s) 804, 1117
HpyCH4V TGCA 4 cut(s) 353, 529, 590, 1334
HpyF10VI GCNNNNNNNGC 5 cut(s) 195, 204, 339, 1340, 1349
HpyF3I CTNAG 2 cut(s) 794, 1230
Hsp92I GRCGYC 2 cut(s) 147, 189
Hsp92II CATG 8 cut(s) 180, 273, 458, 648, 1064, 1073, 1352, 1357
HspAI GCGC 4 cut(s) 6, 189, 515, 539
KasI GGCGCC 1 cut(s) 188
KroI GCCGGC 1 cut(s) 338
KroNI GCCGGC 1 cut(s) 340
KspAI GTTAAC 1 cut(s) 1029
Kzo9I GATC 3 cut(s) 641, 1257, 1261
LmnI GCTCC 2 cut(s) 902, 1310
Lsp1109I GCAGC 3 cut(s) 357, 823, 1190
LweI GCATC 1 cut(s) 222
MaeI CTAG 4 cut(s) 612, 627, 675, 969
MalI GATC 3 cut(s) 643, 1259, 1263
MbiI CCGCTC 1 cut(s) 30
MboI GATC 3 cut(s) 641, 1257, 1261
MboII GAAGA 5 cut(s) 230, 378, 890, 1080, 1164
MfeI CAATTG 1 cut(s) 313
MhlI GDGCHC 2 cut(s) 592, 1315
MluCI AATT 6 cut(s) 300, 313, 460, 490, 895, 1376
Mly113I GGCGCC 1 cut(s) 189
MlyI GAGTC 2 cut(s) 222, 1133
MmeI TCCRAC 3 cut(s) 190, 250, 929
MroNI GCCGGC 1 cut(s) 338
MseI TTAA 3 cut(s) 1028, 1130, 1289
MslI CAYNNNNRTG 1 cut(s) 690
MspI CCGG 2 cut(s) 195, 339
MspR9I CCNGG 2 cut(s) 293, 1245
MunI CAATTG 1 cut(s) 313
Mva1269I GAATGC 1 cut(s) 529
MvaI CCWGG 2 cut(s) 293, 1245
MwoI GCNNNNNNNGC 5 cut(s) 195, 204, 339, 1340, 1349
NaeI GCCGGC 1 cut(s) 340
NarI GGCGCC 1 cut(s) 189
NdeII GATC 3 cut(s) 641, 1257, 1261
NgoMIV GCCGGC 1 cut(s) 338
NlaIII CATG 8 cut(s) 180, 273, 458, 648, 1064, 1073, 1352, 1357
NlaIV GGNNCC 3 cut(s) 190, 694, 1312
NmeAIII GCCGAG 1 cut(s) 183
PasI CCCWGGG 1 cut(s) 292
PcsI WCGNNNNNNNCGW 1 cut(s) 252
PctI GAATGC 1 cut(s) 529
PdiI GCCGGC 1 cut(s) 340
PfeI GAWTC 4 cut(s) 322, 497, 704, 1226
PflFI GACNNNGTC 1 cut(s) 759
PkrI GCNGC 9 cut(s) 82, 85, 124, 209, 212, 344, 347, 838, 1180
PleI GAGTC 2 cut(s) 222, 1132
PluTI GGCGCC 1 cut(s) 192
PpsI GAGTC 2 cut(s) 222, 1132
PshAI GACNNNNGTC 1 cut(s) 435
Psp6I CCWGG 2 cut(s) 291, 1243
PspGI CCWGG 2 cut(s) 291, 1243
PspN4I GGNNCC 3 cut(s) 190, 694, 1312
PspPI GGNCC 3 cut(s) 295, 761, 773
PstNI CAGNNNCTG 1 cut(s) 836
PsyI GACNNNGTC 1 cut(s) 759
RsaI GTAC 2 cut(s) 1047, 1242
RsaNI GTAC 2 cut(s) 1046, 1241
RseI CAYNNNNRTG 1 cut(s) 690
SalI GTCGAC 1 cut(s) 438
SaqAI TTAA 3 cut(s) 1028, 1130, 1289
SatI GCNGC 9 cut(s) 81, 84, 123, 208, 211, 343, 346, 837, 1179
Sau3AI GATC 3 cut(s) 641, 1257, 1261
Sau96I GGNCC 3 cut(s) 295, 761, 773
ScaI AGTACT 1 cut(s) 1047
SchI GAGTC 2 cut(s) 222, 1133
ScrFI CCNGG 2 cut(s) 293, 1245
SduI GDGCHC 2 cut(s) 592, 1315
SfaNI GCATC 1 cut(s) 222
SfoI GGCGCC 1 cut(s) 190
SinI GGWCC 1 cut(s) 761
SmiMI CAYNNNNRTG 1 cut(s) 690
SpeI ACTAGT 1 cut(s) 674
Sse9I AATT 6 cut(s) 300, 313, 460, 490, 895, 1376
SspDI GGCGCC 1 cut(s) 188
SspMI CTAG 4 cut(s) 612, 627, 675, 969
StyD4I CCNGG 2 cut(s) 291, 1243
TaaI ACNGT 2 cut(s) 804, 1117
TaqI TCGA 6 cut(s) 216, 320, 439, 730, 769, 1260
TasI AATT 6 cut(s) 300, 313, 460, 490, 895, 1376
TatI WGTACW 1 cut(s) 1045
TauI GCSGC 6 cut(s) 83, 86, 125, 210, 213, 345
TfiI GAWTC 4 cut(s) 322, 497, 704, 1226
Tru1I TTAA 3 cut(s) 1028, 1130, 1289
Tru9I TTAA 3 cut(s) 1028, 1130, 1289
TscAI CASTG 3 cut(s) 124, 837, 876
TseI GCWGC 3 cut(s) 345, 836, 1178
TspDTI ATGAA 4 cut(s) 750, 1050, 1158, 1389
TspGWI ACGGA 1 cut(s) 716
TspRI CASTG 3 cut(s) 124, 837, 876
Tth111I GACNNNGTC 1 cut(s) 759
VneI GTGCAC 1 cut(s) 588
VpaK11BI GGWCC 1 cut(s) 761
XagI CCTNNNNNAGG 1 cut(s) 768
XapI RAATTY 3 cut(s) 300, 460, 490
XmiI GTMKAC 1 cut(s) 439
XspI CTAG 4 cut(s) 612, 627, 675, 969
ZrmI AGTACT 1 cut(s) 1047
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.