pycom17g12600

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0008300
Physical Location & Seq
Reverse (-)
10306118 .. 10308954
2837 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1401 bp
ATGGCGTCTGGAAGCAAGAGTAATCCCCAATTGCGCAGAGCTCTTTGCTCAAATGGTGAGAAGAGGCAGCCATCGAGTGCGACGGCGCCGTCCCCATCTGTAATAGTGATAGGTGGCGGTATGGCGGGCATTTCAGCTGCTCGGGCTCTTCATGATGCATCAATTCAGGTTGTGCTGTTGGAGTCTCGAGACAGGCTGGGCGGTCGAGTTTGCACCGATTACTCGTTTGGTTTCCCTATTGACTTGGGTGCATCATGGTTGCATGGAGTGTGCAAAGAGAACCCATTGGCACCATTAATTGGGAGGCTGGGGTTACCTTTATACCGTACGAGTGAGGATAACTCTGTCTTATATGACCATGACTTGGAAAGCTATGCACTCTTTGATATGGATGGGAATCAAGTTCCTCAAGATTTGGTCACGAAAGTTGGTGAAGCATTTGAGAAAATTTTGAAAGAGACAGACGCGGTAAGACAGGAATTTAGTGAGGACATGTCCATCACCCGTGCTTTCTCAATTGTTTTTGAAAGGAAACCGGAATTAAGGTTGGAGGGAGTTGCGCATAAGGAAGAACTGCTCCCTGGTGGCCATGGTCTCATGGTCAGGGGTTACCTCCCTGTTATTAACACCCTAGCCAAAGGTCTTGACATACGCTTGAGCCACAGGGTCACAAAAATAACAAGGCGATATAATGGGGTGAAGGTGACAGTAGAGGATGGGAGAACATTTGTTGCGGATGCTGCTGTTGTTGCTGTTCCTCTTGGTGTGCTTAAAGCAAAGAGCATAACGTTTGAGCCGAAGCTACCTGATTGGAAGGAAGCAGCCATTGAAGACCTCGGAGTGGGAATAGAGAATAAAATTGTATTGCACTTCGAAAAGGTGTTTTGGCCAAACGTGGAGTTTTTAGGAGTGGTTGCTGACACATCTTATTGCTGCAGCTACTTTCTAAATCTTCACAAGGCAACTGGTCACTCTGTGCTTGTTTATATGCCTGCAGGGCAGCTGGCTAAAGACATAGAGAAAATGTCTGATGAAGAGGCTGCTAATTTTGCTTTTACGCAACTCAAGAAGATCCTTCCTGATGCTTCTACTCCGATTCAGTATCTTGTTTCTCATTGGGGCAGTGATGTTAACACACTTGGGTCGTATAGCTACGACATAGTTGGAAAACCACATGATCTGTACGAGAAGCTAAGGGTCCCAGTAGATACCCTATTTTTTGCTGGGGAGGCAACAAGTGTTGACTTCCCAGGCTCAGTGCATGGTGCATTCGCAACTGGAGTAATGGCTGCTGAAGACTGCAGAATGCGCGTTCTTGAGCGTTATGGGGAGTTGGATTTATTCGAGCCAGTCATGGGTGAGGAGGCCATATCCATTCCGCTTCTGATCTCCCGCCTGTAA

Protein Analysis

467

Amino Acids

51.07

Weight (kDa)

5.47

Isoelectric Point (pI)

34.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_2 PF00890 34 - 77 9.9e-06 FAD binding domain
DAO PF01266 34 - 252 9.7e-06 FAD dependent oxidoreductase
FAD_oxidored PF12831 34 - 70 2.8e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 36 - 94 1.6e-12 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 41 - 434 8.5e-86 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 88
Acc16I TGCGCA 2 cut(s) 35, 561
AccB1I GGYRCC 2 cut(s) 85, 289
AccB7I CCANNNNNTGG 2 cut(s) 299, 364
AccII CGCG 2 cut(s) 467, 1311
AciI CCGC 7 cut(s) 117, 125, 201, 467, 734, 1379, 1393
AclI AACGTT 1 cut(s) 788
AclWI GGATC 1 cut(s) 1066
AcoI YGGCCR 2 cut(s) 586, 887
AcsI RAATTY 2 cut(s) 447, 479
AcuI CTGAAG 1 cut(s) 1314
AcyI GRCGYC 2 cut(s) 5, 86
AdeI CACNNNGTG 1 cut(s) 976
AfaI GTAC 2 cut(s) 328, 1184
AfiI CCNNNNNNNGG 4 cut(s) 299, 364, 841, 1355
AflIII ACRYGT 1 cut(s) 492
AgsI TTSAA 3 cut(s) 454, 527, 830
AjnI CCWGG 2 cut(s) 580, 1249
AluBI AGCT 8 cut(s) 41, 137, 372, 802, 939, 1003, 1152, 1192
AluI AGCT 8 cut(s) 41, 137, 372, 802, 939, 1003, 1152, 1192
Alw21I GWGCWC 1 cut(s) 43
Alw26I GTCTC 4 cut(s) 183, 189, 452, 599
AlwI GGATC 1 cut(s) 1066
Ama87I CYCGRG 2 cut(s) 141, 186
AoxI GGCC 3 cut(s) 586, 887, 1365
ApeKI GCWGC 9 cut(s) 67, 137, 740, 821, 933, 936, 1000, 1040, 1289
ApoI RAATTY 2 cut(s) 447, 479
AseI ATTAAT 1 cut(s) 296
AspLEI GCGC 4 cut(s) 36, 88, 562, 1311
AspS9I GGNCC 1 cut(s) 1198
AsuHPI GGTGA 6 cut(s) 68, 443, 493, 709, 715, 1370
AsuII TTCGAA 1 cut(s) 873
AvaI CYCGRG 2 cut(s) 141, 186
AvaII GGWCC 1 cut(s) 1198
BalI TGGCCA 2 cut(s) 588, 889
BanI GGYRCC 2 cut(s) 85, 289
BanII GRGCYC 2 cut(s) 43, 148
BbsI GAAGAC 2 cut(s) 837, 1302
Bbv12I GWGCWC 1 cut(s) 43
BbvI GCAGC 9 cut(s) 79, 124, 727, 833, 920, 948, 1012, 1027, 1276
BccI CCATC 5 cut(s) 79, 103, 386, 506, 710
BceAI ACGGC 2 cut(s) 73, 99
BciT130I CCWGG 2 cut(s) 582, 1251
BcoDI GTCTC 4 cut(s) 183, 189, 452, 599
BfaI CTAG 1 cut(s) 632
BfmI CTRYAG 3 cut(s) 934, 993, 1300
BfoI RGCGCY 1 cut(s) 89
BglI GCCNNNNNGGC 1 cut(s) 997
BisI GCNGC 9 cut(s) 68, 138, 741, 822, 934, 937, 1001, 1041, 1290
BlsI GCNGC 9 cut(s) 69, 139, 742, 823, 935, 938, 1002, 1042, 1291
Bme1390I CCNGG 2 cut(s) 582, 1251
Bme18I GGWCC 1 cut(s) 1198
BmeT110I CYCGRG 2 cut(s) 141, 186
BmgT120I GGNCC 1 cut(s) 1198
BmiI GGNNCC 4 cut(s) 87, 291, 1199, 1200
BmrFI CCNGG 2 cut(s) 582, 1251
BmrI ACTGGG 1 cut(s) 1196
BmsI GCATC 5 cut(s) 145, 167, 260, 727, 1072
BmuI ACTGGG 1 cut(s) 1196
BpiI GAAGAC 2 cut(s) 837, 1302
BplI GAGNNNNNCTC 2 cut(s) 326, 358
BpmI CTGGAG 1 cut(s) 1299
Bpu10I CCTNAGC 1 cut(s) 1193
Bpu14I TTCGAA 1 cut(s) 873
BpuEI CTTGAG 4 cut(s) 393, 676, 1049, 1337
BsaBI GATNNNNATC 1 cut(s) 396
BsaHI GRCGYC 2 cut(s) 5, 86
BsaI GGTCTC 1 cut(s) 599
BsaJI CCNNGG 4 cut(s) 580, 589, 835, 1249
BsaWI WCCGGW 1 cut(s) 535
Bsc4I CCNNNNNNNGG 4 cut(s) 299, 364, 841, 1355
Bse1I ACTGG 4 cut(s) 970, 1202, 1282, 1349
Bse8I GATNNNNATC 1 cut(s) 396
BseBI CCWGG 2 cut(s) 582, 1251
BseDI CCNNGG 4 cut(s) 580, 589, 835, 1249
BseGI GGATG 3 cut(s) 397, 721, 742
BseJI GATNNNNATC 1 cut(s) 396
BseLI CCNNNNNNNGG 4 cut(s) 299, 364, 841, 1355
BseMII CTCAG 1 cut(s) 1269
BseNI ACTGG 4 cut(s) 970, 1202, 1282, 1349
BseRI GAGGAG 1 cut(s) 1376
BseXI GCAGC 9 cut(s) 79, 124, 727, 833, 920, 948, 1012, 1027, 1276
BseYI CCCAGC 3 cut(s) 196, 307, 1223
Bsh1236I CGCG 2 cut(s) 467, 1311
Bsh1285I CGRYCG 1 cut(s) 205
BshFI GGCC 3 cut(s) 588, 889, 1367
BshNI GGYRCC 2 cut(s) 85, 289
BsiEI CGRYCG 1 cut(s) 205
BsiHKAI GWGCWC 1 cut(s) 43
BsiHKCI CYCGRG 2 cut(s) 141, 186
BsiSI CCGG 1 cut(s) 536
BsiWI CGTACG 1 cut(s) 326
BslFI GGGAC 2 cut(s) 76, 1184
BslI CCNNNNNNNGG 4 cut(s) 299, 364, 841, 1355
BsmAI GTCTC 4 cut(s) 183, 189, 452, 599
BsmFI GGGAC 2 cut(s) 76, 1184
BsmI GAATGC 2 cut(s) 1268, 1311
BsnI GGCC 3 cut(s) 588, 889, 1367
Bso31I GGTCTC 1 cut(s) 599
BsoBI CYCGRG 2 cut(s) 141, 186
Bsp119I TTCGAA 1 cut(s) 873
Bsp1286I GDGCHC 2 cut(s) 43, 148
Bsp143I GATC 3 cut(s) 1071, 1177, 1386
Bsp19I CCATGG 1 cut(s) 589
BspACI CCGC 7 cut(s) 117, 125, 201, 467, 734, 1379, 1393
BspANI GGCC 3 cut(s) 588, 889, 1367
BspCNI CTCAG 1 cut(s) 1268
BspFNI CGCG 2 cut(s) 467, 1311
BspHI TCATGA 1 cut(s) 151
BspLI GGNNCC 4 cut(s) 87, 291, 1199, 1200
BspMAI CTGCAG 3 cut(s) 938, 997, 1304
BspPI GGATC 1 cut(s) 1066
BspQI GCTCTTC 1 cut(s) 153
BspT104I TTCGAA 1 cut(s) 873
BspT107I GGYRCC 2 cut(s) 85, 289
BspTNI GGTCTC 1 cut(s) 599
BsrI ACTGG 4 cut(s) 970, 1202, 1282, 1349
BssECI CCNNGG 4 cut(s) 580, 589, 835, 1249
BssMI GATC 3 cut(s) 1071, 1177, 1386
BssNI GRCGYC 2 cut(s) 5, 86
BssT1I CCWWGG 1 cut(s) 589
Bst2UI CCWGG 2 cut(s) 582, 1251
Bst4CI ACNGT 2 cut(s) 326, 709
Bst6I CTCTTC 3 cut(s) 56, 153, 1029
BstACI GRCGYC 2 cut(s) 5, 86
BstBI TTCGAA 1 cut(s) 873
BstC8I GCNNGC 3 cut(s) 127, 993, 1005
BstDEI CTNAG 2 cut(s) 1193, 1255
BstDSI CCRYGG 1 cut(s) 589
BstEII GGTNACC 2 cut(s) 312, 608
BstF5I GGATG 3 cut(s) 397, 721, 742
BstFNI CGCG 2 cut(s) 467, 1311
BstH2I RGCGCY 1 cut(s) 89
BstHHI GCGC 4 cut(s) 36, 88, 562, 1311
BstKTI GATC 3 cut(s) 1074, 1180, 1389
BstMAI GTCTC 4 cut(s) 183, 189, 452, 599
BstMBI GATC 3 cut(s) 1071, 1177, 1386
BstMCI CGRYCG 1 cut(s) 205
BstMWI GCNNNNNNNGC 7 cut(s) 143, 740, 749, 997, 1049, 1229, 1308
BstNI CCWGG 2 cut(s) 582, 1251
BstNSI RCATGY 1 cut(s) 496
BstPI GGTNACC 2 cut(s) 312, 608
BstSCI CCNGG 2 cut(s) 580, 1249
BstSFI CTRYAG 3 cut(s) 934, 993, 1300
BstUI CGCG 2 cut(s) 467, 1311
BstV1I GCAGC 9 cut(s) 79, 124, 727, 833, 920, 948, 1012, 1027, 1276
BstV2I GAAGAC 2 cut(s) 837, 1302
BstX2I RGATCY 1 cut(s) 1071
BstYI RGATCY 1 cut(s) 1071
BsuRI GGCC 3 cut(s) 588, 889, 1367
BtgI CCRYGG 1 cut(s) 589
BtsCI GGATG 3 cut(s) 397, 721, 742
BtsI GCAGTG 1 cut(s) 1129
BtsIMutI CAGTG 2 cut(s) 1129, 1263
Cac8I GCNNGC 3 cut(s) 127, 993, 1005
CciI TCATGA 1 cut(s) 151
CfoI GCGC 4 cut(s) 36, 88, 562, 1311
Cfr13I GGNCC 1 cut(s) 1198
CseI GACGC 1 cut(s) 473
Csp6I GTAC 2 cut(s) 327, 1183
CviQI GTAC 2 cut(s) 327, 1183
DdeI CTNAG 2 cut(s) 1193, 1255
DinI GGCGCC 1 cut(s) 87
DpnI GATC 3 cut(s) 1073, 1179, 1388
DpnII GATC 3 cut(s) 1071, 1177, 1386
DraIII CACNNNGTG 1 cut(s) 976
DrdI GACNNNNNNGTC 1 cut(s) 88
DseDI GACNNNNNNGTC 1 cut(s) 88
EaeI YGGCCR 2 cut(s) 586, 887
Eam1104I CTCTTC 3 cut(s) 56, 153, 1029
EarI CTCTTC 3 cut(s) 56, 153, 1029
Ecl136II GAGCTC 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 589
Eco24I GRGCYC 2 cut(s) 43, 148
Eco31I GGTCTC 1 cut(s) 599
Eco47I GGWCC 1 cut(s) 1198
Eco53kI GAGCTC 1 cut(s) 41
Eco57I CTGAAG 1 cut(s) 1314
Eco88I CYCGRG 2 cut(s) 141, 186
Eco91I GGTNACC 2 cut(s) 312, 608
EcoICRI GAGCTC 1 cut(s) 41
EcoO109I RGGNCCY 1 cut(s) 1198
EcoO65I GGTNACC 2 cut(s) 312, 608
EcoRII CCWGG 2 cut(s) 580, 1249
EcoT14I CCWWGG 1 cut(s) 589
EcoT22I ATGCAT 1 cut(s) 160
EcoT38I GRGCYC 2 cut(s) 43, 148
EgeI GGCGCC 1 cut(s) 87
EheI GGCGCC 1 cut(s) 87
ErhI CCWWGG 1 cut(s) 589
FaqI GGGAC 2 cut(s) 76, 1184
FauI CCCGC 1 cut(s) 118
Fnu4HI GCNGC 9 cut(s) 68, 138, 741, 822, 934, 937, 1001, 1041, 1290
FokI GGATG 3 cut(s) 404, 728, 749
FriOI GRGCYC 2 cut(s) 43, 148
Fsp4HI GCNGC 9 cut(s) 68, 138, 741, 822, 934, 937, 1001, 1041, 1290
FspBI CTAG 1 cut(s) 632
FspI TGCGCA 2 cut(s) 35, 561
GlaI GCGC 4 cut(s) 35, 87, 561, 1310
GluI GCNGC 9 cut(s) 68, 138, 741, 822, 934, 937, 1001, 1041, 1290
GsaI CCCAGC 3 cut(s) 200, 311, 1227
GsuI CTGGAG 1 cut(s) 1299
HaeII RGCGCY 1 cut(s) 89
HaeIII GGCC 3 cut(s) 588, 889, 1367
HapII CCGG 1 cut(s) 536
HgaI GACGC 1 cut(s) 473
HhaI GCGC 4 cut(s) 36, 88, 562, 1311
Hin1I GRCGYC 2 cut(s) 5, 86
Hin6I GCGC 4 cut(s) 34, 86, 560, 1309
HinP1I GCGC 4 cut(s) 34, 86, 560, 1309
HincII GTYRAC 2 cut(s) 1132, 1243
HindII GTYRAC 2 cut(s) 1132, 1243
HinfI GANTC 3 cut(s) 182, 397, 1096
HpaI GTTAAC 1 cut(s) 1132
HpaII CCGG 1 cut(s) 536
HphI GGTGA 6 cut(s) 68, 443, 493, 709, 715, 1370
Hpy166II GTNNAC 2 cut(s) 1132, 1243
Hpy188I TCNGA 4 cut(s) 839, 1030, 1095, 1386
Hpy8I GTNNAC 2 cut(s) 1132, 1243
Hpy99I CGWCG 1 cut(s) 85
HpyAV CCTTC 3 cut(s) 694, 808, 1085
HpyCH4III ACNGT 2 cut(s) 326, 709
HpyCH4IV ACGT 2 cut(s) 788, 894
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 740, 749, 997, 1049, 1229, 1308
HpyF3I CTNAG 2 cut(s) 1193, 1255
HpySE526I ACGT 2 cut(s) 788, 894
Hsp92I GRCGYC 2 cut(s) 5, 86
HspAI GCGC 4 cut(s) 34, 86, 560, 1309
KasI GGCGCC 1 cut(s) 85
KflI GGGWCCC 1 cut(s) 1198
KspAI GTTAAC 1 cut(s) 1132
Kzo9I GATC 3 cut(s) 1071, 1177, 1386
LguI GCTCTTC 1 cut(s) 153
LmnI GCTCC 1 cut(s) 582
Lsp1109I GCAGC 9 cut(s) 79, 124, 727, 833, 920, 948, 1012, 1027, 1276
LweI GCATC 5 cut(s) 145, 167, 260, 727, 1072
MaeI CTAG 1 cut(s) 632
MaeII ACGT 2 cut(s) 788, 894
MaeIII GTNAC 6 cut(s) 312, 418, 608, 667, 703, 968
MalI GATC 3 cut(s) 1073, 1179, 1388
MboI GATC 3 cut(s) 1071, 1177, 1386
MboII GAAGA 8 cut(s) 73, 140, 581, 842, 944, 1046, 1081, 1307
MfeI CAATTG 2 cut(s) 29, 516
MflI RGATCY 1 cut(s) 1071
MhlI GDGCHC 2 cut(s) 43, 148
MlsI TGGCCA 2 cut(s) 588, 889
MluCI AATT 9 cut(s) 29, 162, 297, 447, 479, 516, 539, 858, 1045
MluNI TGGCCA 2 cut(s) 588, 889
Mly113I GGCGCC 1 cut(s) 86
MlyI GAGTC 1 cut(s) 191
MmeI TCCRAC 4 cut(s) 159, 528, 1144, 1314
Mox20I TGGCCA 2 cut(s) 588, 889
Mph1103I ATGCAT 1 cut(s) 160
MscI TGGCCA 2 cut(s) 588, 889
MseI TTAA 5 cut(s) 296, 542, 624, 771, 1131
Msp20I TGGCCA 2 cut(s) 588, 889
MspA1I CMGCKG 2 cut(s) 137, 1003
MspI CCGG 1 cut(s) 536
MspR9I CCNGG 2 cut(s) 582, 1251
MunI CAATTG 2 cut(s) 29, 516
Mva1269I GAATGC 2 cut(s) 1268, 1311
MvaI CCWGG 2 cut(s) 582, 1251
MvnI CGCG 2 cut(s) 467, 1311
MwoI GCNNNNNNNGC 7 cut(s) 143, 740, 749, 997, 1049, 1229, 1308
NarI GGCGCC 1 cut(s) 86
NcoI CCATGG 1 cut(s) 589
NdeII GATC 3 cut(s) 1071, 1177, 1386
NlaIV GGNNCC 4 cut(s) 87, 291, 1199, 1200
NmuCI GTSAC 4 cut(s) 418, 667, 703, 968
NsbI TGCGCA 2 cut(s) 35, 561
NsiI ATGCAT 1 cut(s) 160
NspI RCATGY 1 cut(s) 496
NspV TTCGAA 1 cut(s) 873
PaeR7I CTCGAG 1 cut(s) 186
PagI TCATGA 1 cut(s) 151
PciI ACATGT 1 cut(s) 492
PciSI GCTCTTC 1 cut(s) 153
PcsI WCGNNNNNNNCGW 1 cut(s) 794
PctI GAATGC 2 cut(s) 1268, 1311
PfeI GAWTC 2 cut(s) 397, 1096
Pfl23II CGTACG 1 cut(s) 326
PflMI CCANNNNNTGG 2 cut(s) 299, 364
PkrI GCNGC 9 cut(s) 69, 139, 742, 823, 935, 938, 1002, 1042, 1291
PleI GAGTC 1 cut(s) 190
PluTI GGCGCC 1 cut(s) 89
PpsI GAGTC 1 cut(s) 190
PpuMI RGGWCCY 1 cut(s) 1198
PscI ACATGT 1 cut(s) 492
PshBI ATTAAT 1 cut(s) 296
Psp124BI GAGCTC 1 cut(s) 43
Psp1406I AACGTT 1 cut(s) 788
Psp5II RGGWCCY 1 cut(s) 1198
Psp6I CCWGG 2 cut(s) 580, 1249
PspEI GGTNACC 2 cut(s) 312, 608
PspFI CCCAGC 3 cut(s) 196, 307, 1223
PspGI CCWGG 2 cut(s) 580, 1249
PspLI CGTACG 1 cut(s) 326
PspN4I GGNNCC 4 cut(s) 87, 291, 1199, 1200
PspPI GGNCC 1 cut(s) 1198
PspPPI RGGWCCY 1 cut(s) 1198
PstI CTGCAG 3 cut(s) 938, 997, 1304
PsuI RGATCY 1 cut(s) 1071
PvuII CAGCTG 2 cut(s) 137, 1003
RsaI GTAC 2 cut(s) 328, 1184
RsaNI GTAC 2 cut(s) 327, 1183
SacI GAGCTC 1 cut(s) 43
SapI GCTCTTC 1 cut(s) 153
SaqAI TTAA 5 cut(s) 296, 542, 624, 771, 1131
SatI GCNGC 9 cut(s) 68, 138, 741, 822, 934, 937, 1001, 1041, 1290
Sau3AI GATC 3 cut(s) 1071, 1177, 1386
Sau96I GGNCC 1 cut(s) 1198
SbfI CCTGCAGG 1 cut(s) 997
SchI GAGTC 1 cut(s) 191
ScrFI CCNGG 2 cut(s) 582, 1251
SdaI CCTGCAGG 1 cut(s) 997
SduI GDGCHC 2 cut(s) 43, 148
SfaNI GCATC 5 cut(s) 145, 167, 260, 727, 1072
SfcI CTRYAG 3 cut(s) 934, 993, 1300
SfoI GGCGCC 1 cut(s) 87
Sfr274I CTCGAG 1 cut(s) 186
SfuI TTCGAA 1 cut(s) 873
SinI GGWCC 1 cut(s) 1198
SlaI CTCGAG 1 cut(s) 186
SmlI CTYRAG 5 cut(s) 186, 408, 655, 1064, 1316
SmoI CTYRAG 5 cut(s) 186, 408, 655, 1064, 1316
Sse8387I CCTGCAGG 1 cut(s) 997
Sse9I AATT 9 cut(s) 29, 162, 297, 447, 479, 516, 539, 858, 1045
SsiI CCGC 7 cut(s) 117, 125, 201, 467, 734, 1379, 1393
SspDI GGCGCC 1 cut(s) 85
SspMI CTAG 1 cut(s) 632
SstI GAGCTC 1 cut(s) 43
StyD4I CCNGG 2 cut(s) 580, 1249
StyI CCWWGG 1 cut(s) 589
TaaI ACNGT 2 cut(s) 326, 709
TaiI ACGT 2 cut(s) 791, 897
TaqI TCGA 5 cut(s) 74, 187, 205, 873, 1344
TasI AATT 9 cut(s) 29, 162, 297, 447, 479, 516, 539, 858, 1045
TfiI GAWTC 2 cut(s) 397, 1096
Tru1I TTAA 5 cut(s) 296, 542, 624, 771, 1131
Tru9I TTAA 5 cut(s) 296, 542, 624, 771, 1131
TscAI CASTG 2 cut(s) 1129, 1263
TseFI GTSAC 4 cut(s) 418, 667, 703, 968
TseI GCWGC 9 cut(s) 67, 137, 740, 821, 933, 936, 1000, 1040, 1289
Tsp45I GTSAC 4 cut(s) 418, 667, 703, 968
TspDTI ATGAA 2 cut(s) 140, 1047
TspRI CASTG 2 cut(s) 1129, 1263
Van91I CCANNNNNTGG 2 cut(s) 299, 364
VpaK11BI GGWCC 1 cut(s) 1198
VspI ATTAAT 1 cut(s) 296
XapI RAATTY 2 cut(s) 447, 479
XceI RCATGY 1 cut(s) 496
XhoI CTCGAG 1 cut(s) 186
XspI CTAG 1 cut(s) 632
Zsp2I ATGCAT 1 cut(s) 160
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.