RLG00000020644

Polyamine oxidase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008300
Physical Location & Seq
Forward (+)
67084898 .. 67087718
2821 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020644

Sequence Viewer

Length: 1509 bp
ATGGCTGGAGGCAAGAGTAATAGTGATAGTAATAGTAATATTACTCCACAATTGCGCAGAGCTGTTTGCAATTCCAATGTTGAGAGGAGGCCGACATCGACATCGACATCGACGCCGTCTCCATCTGTGATAGTGATAGGTGGAGGCATGGCTGGTATTGCAGCTGCTCGTGCTCTCCAAGATGCATCATTCCAGGTTGTGCTGTTGGAGTCTCGGGATCGACTTGGCGGTCGAGTTCACACCGATTACTCGTTTGGTTTTCCGATTGACTTGGGTGCATCATGGTTGCATGGAGTATGTAAAGAGAATCCCTTGGCTCCATTAATCGGGAGACTGGGCTTGCCTTTATATCGAACCAGTGGTGATAACTCTGTGTTGTATGATCATGACTTAGAAAGCTATGCACTCTTTGATATGGATGGGAAACAAGTTCCTCAAGATTTGGTTACAAAAGTTGGTGTAAGATTTGAGGAAATTTTAAAAGAGGCAAATGAGGTAAGAAAGGAACTCTCAGAAGACATGTCCATCACCCGCGCTTTCTCAATTGTCTTTGAAAGGAAACCAGAATTAAGGTTGGAAGGAATTTCCCATAAGGTACTGCAGTGGTATTTGTGCCGAATGGAAGGCTGGTTTGCAGCAGATGCTGATACAATTTCACTTAAAAGCTGGGATCAGGAAGAACTGCTTCCTGGTGGTCATGGTCTTATGGTCAGGGGCTATCGCCCTGTTATAAACACCCTTGCCAAAGGTCTTGACATTCGCCTGAGCCACAGGGTGACAAAAATTTTGAGGCGATATAATGGTGTGATGGTAACAGTAGAGGATGGGAGATCATTTGTTGCAGATGCTGCAATTATTGCTGTTCCTCTTGGTGTGCTTAAGGCAAATACCATAAAGTTTGAGCCAAAGCTACCTGACTGGAAGGAAGCAGCCATTGCTGACCTTGGAGTGGGAATTGAGAATAAGATAGTGTTGCACTTTGAGAAGGTGTTCTGGCCAAATGTGGAGTTTTTAGGAGTGGTTGCGGAGACGTCTTACTGTTGCAGCTACTTTCTAAATCTTCACAAGGCAACTGGTCACTCTGTGCTTGTTTATATGCCTGCAGGGCAGCTGGCCAGAGACATTGAGAAAATGTCCGATGAAGAAGCTGCTAATTTTGCATATATGCAACTCAAGAAGATCCTTCCAGATGCTTCTGATCCGATTCATTTTCTTGTTTCTCACTGGGGAACAGATATAAATTCCCTTGGGTCCTATAGCTATGATATGGTAGGCAAACCCCATGATCTATATGAGAAGCTAAGGGTCCCGGTAGATAACCTTTTCTTTGCTGGGGAGGCAACAAGTGTGGACTTCCCAGGTTCTGTGCATGGTGCATTTGCTACTGGAGCAATGGCTGCTGAAGACTGCAGGATGCGTGTTCTGGAGCGATACGGTGAGTTGGATTTGTTCGAGCCAGTCATGGGTGAGGAGGTCATGTCTATCCCAATTCAGATCTCCCGCATGTAA

Protein Analysis

503

Amino Acids

55.58

Weight (kDa)

5.54

Isoelectric Point (pI)

36.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
FAD_binding_2 PF00890 43 - 86 1.4e-06 FAD binding domain
DAO PF01266 43 - 77 2.1e-06 FAD dependent oxidoreductase
FAD_oxidored PF12831 43 - 78 1.7e-06 FAD dependent oxidoreductase
NAD_binding_8 PF13450 45 - 103 2.8e-14 NAD(P)-binding Rossmann-like domain
Amino_oxidase PF01593 50 - 470 3.4e-97 Flavin containing amine oxidoreductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 731
AasI GACNNNNNNGTC 1 cut(s) 228
AatII GACGTC 1 cut(s) 1034
Acc16I TGCGCA 1 cut(s) 56
AccII CGCG 1 cut(s) 534
AciI CCGC 4 cut(s) 228, 532, 1025, 1501
AclWI GGATC 4 cut(s) 225, 678, 1174, 1193
AcoI YGGCCR 2 cut(s) 995, 1113
AcsI RAATTY 4 cut(s) 474, 582, 783, 1240
AcuI CTGAAG 1 cut(s) 1422
AcyI GRCGYC 2 cut(s) 113, 1031
AdeI CACNNNGTG 2 cut(s) 775, 1084
AfaI GTAC 1 cut(s) 597
AfiI CCNNNNNNNGG 3 cut(s) 326, 949, 1463
AflII CTTAAG 1 cut(s) 878
AflIII ACRYGT 1 cut(s) 519
AgsI TTSAA 1 cut(s) 554
AjnI CCWGG 3 cut(s) 192, 688, 1357
Alw21I GWGCWC 1 cut(s) 175
Alw26I GTCTC 5 cut(s) 123, 216, 325, 1022, 1113
AlwI GGATC 4 cut(s) 225, 678, 1174, 1193
AlwNI CAGNNNCTG 3 cut(s) 644, 848, 1364
Ama87I CYCGRG 1 cut(s) 213
AoxI GGCC 3 cut(s) 89, 995, 1113
ApeKI GCWGC 9 cut(s) 161, 164, 635, 848, 929, 1044, 1108, 1148, 1397
ApoI RAATTY 4 cut(s) 474, 582, 783, 1240
ArsI GACNNNNNNTTYG 2 cut(s) 769, 801
AseI ATTAAT 1 cut(s) 323
Asp700I GAANNNNTTC 2 cut(s) 684, 989
AspLEI GCGC 2 cut(s) 57, 536
AspS9I GGNCC 2 cut(s) 1251, 1306
AsuC2I CCSGG 1 cut(s) 1310
AsuHPI GGTGA 5 cut(s) 374, 520, 787, 1448, 1478
AvaI CYCGRG 1 cut(s) 213
AvaII GGWCC 2 cut(s) 1251, 1306
BaeI ACNNNNGTAYC 2 cut(s) 587, 620
BalI TGGCCA 2 cut(s) 997, 1115
BauI CACGAG 1 cut(s) 168
BbsI GAAGAC 2 cut(s) 522, 1410
Bbv12I GWGCWC 1 cut(s) 175
BbvI GCAGC 9 cut(s) 151, 173, 647, 835, 941, 1056, 1120, 1135, 1384
BccI CCATC 5 cut(s) 130, 413, 533, 802, 818
BceAI ACGGC 1 cut(s) 100
BciT130I CCWGG 3 cut(s) 194, 690, 1359
BclI TGATCA 1 cut(s) 382
BcnI CCSGG 1 cut(s) 1310
BcoDI GTCTC 5 cut(s) 123, 216, 325, 1022, 1113
BfmI CTRYAG 4 cut(s) 599, 1101, 1255, 1408
BfrI CTTAAG 1 cut(s) 878
BglI GCCNNNNNGGC 1 cut(s) 1105
BglII AGATCT 1 cut(s) 1494
BisI GCNGC 9 cut(s) 162, 165, 636, 849, 930, 1045, 1109, 1149, 1398
BlsI GCNGC 9 cut(s) 163, 166, 637, 850, 931, 1046, 1110, 1150, 1399
Bme1390I CCNGG 4 cut(s) 194, 690, 1310, 1359
Bme18I GGWCC 2 cut(s) 1251, 1306
BmeT110I CYCGRG 1 cut(s) 213
BmgT120I GGNCC 2 cut(s) 1251, 1306
BmiI GGNNCC 4 cut(s) 318, 1252, 1307, 1308
BmrFI CCNGG 4 cut(s) 194, 690, 1310, 1359
BmrI ACTGGG 2 cut(s) 344, 1234
BmsI GCATC 7 cut(s) 172, 194, 287, 631, 835, 1180, 1404
BmuI ACTGGG 2 cut(s) 344, 1234
BpiI GAAGAC 2 cut(s) 522, 1410
BpmI CTGGAG 3 cut(s) 27, 1407, 1445
Bpu10I CCTNAGC 2 cut(s) 764, 1301
BpuEI CTTGAG 2 cut(s) 420, 1157
BpuMI CCSGG 1 cut(s) 1310
BsaHI GRCGYC 2 cut(s) 113, 1031
BsaJI CCNNGG 4 cut(s) 312, 943, 1246, 1357
BsaXI ACNNNNNCTCC 4 cut(s) 103, 133, 1463, 1493
Bsc4I CCNNNNNNNGG 3 cut(s) 326, 949, 1463
Bse1I ACTGG 7 cut(s) 339, 357, 923, 1078, 1229, 1390, 1457
Bse3DI GCAATG 2 cut(s) 933, 1398
BseBI CCWGG 3 cut(s) 194, 690, 1359
BseDI CCNNGG 4 cut(s) 312, 943, 1246, 1357
BseGI GGATG 3 cut(s) 424, 829, 1419
BseLI CCNNNNNNNGG 3 cut(s) 326, 949, 1463
BseMI GCAATG 2 cut(s) 933, 1398
BseMII CTCAG 2 cut(s) 525, 755
BseNI ACTGG 7 cut(s) 339, 357, 923, 1078, 1229, 1390, 1457
BseRI GAGGAG 2 cut(s) 100, 1484
BseXI GCAGC 9 cut(s) 151, 173, 647, 835, 941, 1056, 1120, 1135, 1384
BseYI CCCAGC 2 cut(s) 666, 1331
Bsh1236I CGCG 1 cut(s) 534
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 3 cut(s) 91, 997, 1115
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 175
BsiHKCI CYCGRG 1 cut(s) 213
BsiSI CCGG 1 cut(s) 1310
BslFI GGGAC 1 cut(s) 1292
BslI CCNNNNNNNGG 3 cut(s) 326, 949, 1463
BsmAI GTCTC 5 cut(s) 123, 216, 325, 1022, 1113
BsmBI CGTCTC 2 cut(s) 123, 1022
BsmFI GGGAC 1 cut(s) 1292
BsnI GGCC 3 cut(s) 91, 997, 1115
BsoBI CYCGRG 1 cut(s) 213
Bsp1286I GDGCHC 1 cut(s) 175
Bsp143I GATC 8 cut(s) 217, 382, 670, 830, 1179, 1198, 1285, 1494
BspACI CCGC 4 cut(s) 228, 532, 1025, 1501
BspANI GGCC 3 cut(s) 91, 997, 1115
BspCNI CTCAG 2 cut(s) 524, 756
BspFNI CGCG 1 cut(s) 534
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 4 cut(s) 318, 1252, 1307, 1308
BspMAI CTGCAG 3 cut(s) 603, 1105, 1412
BspPI GGATC 4 cut(s) 225, 678, 1174, 1193
BspTI CTTAAG 1 cut(s) 878
BsrDI GCAATG 2 cut(s) 933, 1398
BsrI ACTGG 7 cut(s) 339, 357, 923, 1078, 1229, 1390, 1457
BssECI CCNNGG 4 cut(s) 312, 943, 1246, 1357
BssMI GATC 8 cut(s) 217, 382, 670, 830, 1179, 1198, 1285, 1494
BssNI GRCGYC 2 cut(s) 113, 1031
BssSI CACGAG 1 cut(s) 168
BssT1I CCWWGG 3 cut(s) 312, 943, 1246
Bst2BI CACGAG 1 cut(s) 168
Bst2UI CCWGG 3 cut(s) 194, 690, 1359
Bst4CI ACNGT 3 cut(s) 817, 1040, 1436
BstACI GRCGYC 2 cut(s) 113, 1031
BstAFI CTTAAG 1 cut(s) 878
BstAPI GCANNNNNTGC 5 cut(s) 641, 848, 857, 935, 1397
BstC8I GCNNGC 3 cut(s) 341, 1101, 1113
BstDEI CTNAG 4 cut(s) 391, 511, 764, 1301
BstF5I GGATG 3 cut(s) 424, 829, 1419
BstFNI CGCG 1 cut(s) 534
BstHHI GCGC 2 cut(s) 57, 536
BstKTI GATC 8 cut(s) 220, 385, 673, 833, 1182, 1201, 1288, 1497
BstMAI GTCTC 5 cut(s) 123, 216, 325, 1022, 1113
BstMBI GATC 8 cut(s) 217, 382, 670, 830, 1179, 1198, 1285, 1494
BstMCI CGRYCG 1 cut(s) 232
BstNI CCWGG 3 cut(s) 194, 690, 1359
BstNSI RCATGY 2 cut(s) 523, 1507
BstSCI CCNGG 4 cut(s) 192, 688, 1308, 1357
BstSFI CTRYAG 4 cut(s) 599, 1101, 1255, 1408
BstUI CGCG 1 cut(s) 534
BstV1I GCAGC 9 cut(s) 151, 173, 647, 835, 941, 1056, 1120, 1135, 1384
BstV2I GAAGAC 2 cut(s) 522, 1410
BstX2I RGATCY 2 cut(s) 1179, 1494
BstYI RGATCY 2 cut(s) 1179, 1494
BsuRI GGCC 3 cut(s) 91, 997, 1115
BtsCI GGATG 3 cut(s) 424, 829, 1419
BtsI GCAGTG 1 cut(s) 608
BtsIMutI CAGTG 3 cut(s) 364, 608, 1222
Cac8I GCNNGC 3 cut(s) 341, 1101, 1113
CaiI CAGNNNCTG 3 cut(s) 644, 848, 1364
CciI TCATGA 1 cut(s) 385
CfoI GCGC 2 cut(s) 57, 536
Cfr13I GGNCC 2 cut(s) 1251, 1306
CseI GACGC 1 cut(s) 121
Csp6I GTAC 1 cut(s) 596
CspCI CAANNNNNGTGG 2 cut(s) 1329, 1364
CviQI GTAC 1 cut(s) 596
DdeI CTNAG 4 cut(s) 391, 511, 764, 1301
DpnI GATC 8 cut(s) 219, 384, 672, 832, 1181, 1200, 1287, 1496
DpnII GATC 8 cut(s) 217, 382, 670, 830, 1179, 1198, 1285, 1494
DraI TTTAAA 1 cut(s) 480
DraIII CACNNNGTG 2 cut(s) 775, 1084
DrdI GACNNNNNNGTC 1 cut(s) 228
DseDI GACNNNNNNGTC 1 cut(s) 228
EaeI YGGCCR 2 cut(s) 995, 1113
Eco130I CCWWGG 3 cut(s) 312, 943, 1246
Eco47I GGWCC 2 cut(s) 1251, 1306
Eco57I CTGAAG 1 cut(s) 1422
Eco88I CYCGRG 1 cut(s) 213
EcoO109I RGGNCCY 2 cut(s) 1251, 1306
EcoRII CCWGG 3 cut(s) 192, 688, 1357
EcoT14I CCWWGG 3 cut(s) 312, 943, 1246
EcoT22I ATGCAT 1 cut(s) 187
ErhI CCWWGG 3 cut(s) 312, 943, 1246
Esp3I CGTCTC 2 cut(s) 123, 1022
FalI AAGNNNNNCTT 2 cut(s) 669, 701
FaqI GGGAC 1 cut(s) 1292
FauI CCCGC 1 cut(s) 539
FbaI TGATCA 1 cut(s) 382
Fnu4HI GCNGC 9 cut(s) 162, 165, 636, 849, 930, 1045, 1109, 1149, 1398
FokI GGATG 3 cut(s) 431, 836, 1426
Fsp4HI GCNGC 9 cut(s) 162, 165, 636, 849, 930, 1045, 1109, 1149, 1398
FspI TGCGCA 1 cut(s) 56
GlaI GCGC 2 cut(s) 56, 535
GluI GCNGC 9 cut(s) 162, 165, 636, 849, 930, 1045, 1109, 1149, 1398
GsaI CCCAGC 2 cut(s) 670, 1335
GsuI CTGGAG 3 cut(s) 27, 1407, 1445
HaeIII GGCC 3 cut(s) 91, 997, 1115
HapII CCGG 1 cut(s) 1310
HgaI GACGC 1 cut(s) 121
HhaI GCGC 2 cut(s) 57, 536
Hin1I GRCGYC 2 cut(s) 113, 1031
Hin6I GCGC 2 cut(s) 55, 534
HinP1I GCGC 2 cut(s) 55, 534
HinfI GANTC 3 cut(s) 209, 307, 1204
HpaII CCGG 1 cut(s) 1310
HphI GGTGA 5 cut(s) 374, 520, 787, 1448, 1478
Hpy166II GTNNAC 2 cut(s) 238, 1351
Hpy188I TCNGA 6 cut(s) 264, 514, 1138, 1198, 1203, 1494
Hpy188III TCNNGA 9 cut(s) 215, 328, 386, 437, 674, 752, 1174, 1187, 1424
Hpy8I GTNNAC 2 cut(s) 238, 1351
Hpy99I CGWCG 1 cut(s) 115
HpyAV CCTTC 5 cut(s) 572, 617, 916, 979, 1193
HpyCH4III ACNGT 3 cut(s) 817, 1040, 1436
HpyCH4IV ACGT 1 cut(s) 1031
HpyF3I CTNAG 4 cut(s) 391, 511, 764, 1301
HpySE526I ACGT 1 cut(s) 1031
Hsp92I GRCGYC 2 cut(s) 113, 1031
HspAI GCGC 2 cut(s) 55, 534
KflI GGGWCCC 1 cut(s) 1306
Ksp22I TGATCA 1 cut(s) 382
Kzo9I GATC 8 cut(s) 217, 382, 670, 830, 1179, 1198, 1285, 1494
LmnI GCTCC 3 cut(s) 322, 1388, 1426
Lsp1109I GCAGC 9 cut(s) 151, 173, 647, 835, 941, 1056, 1120, 1135, 1384
LweI GCATC 7 cut(s) 172, 194, 287, 631, 835, 1180, 1404
MaeII ACGT 1 cut(s) 1031
MaeIII GTNAC 4 cut(s) 445, 775, 811, 1076
MalI GATC 8 cut(s) 219, 384, 672, 832, 1181, 1200, 1287, 1496
MboI GATC 8 cut(s) 217, 382, 670, 830, 1179, 1198, 1285, 1494
MboII GAAGA 6 cut(s) 527, 689, 1052, 1154, 1189, 1415
MfeI CAATTG 2 cut(s) 50, 543
MflI RGATCY 2 cut(s) 1179, 1494
MhlI GDGCHC 1 cut(s) 175
MlsI TGGCCA 2 cut(s) 997, 1115
MluNI TGGCCA 2 cut(s) 997, 1115
MlyI GAGTC 1 cut(s) 218
MmeI TCCRAC 3 cut(s) 186, 555, 1422
Mox20I TGGCCA 2 cut(s) 997, 1115
Mph1103I ATGCAT 1 cut(s) 187
MroXI GAANNNNTTC 2 cut(s) 684, 989
MscI TGGCCA 2 cut(s) 997, 1115
MseI TTAA 5 cut(s) 323, 479, 569, 660, 879
Msp20I TGGCCA 2 cut(s) 997, 1115
MspA1I CMGCKG 2 cut(s) 164, 1111
MspCI CTTAAG 1 cut(s) 878
MspI CCGG 1 cut(s) 1310
MspR9I CCNGG 4 cut(s) 194, 690, 1310, 1359
MunI CAATTG 2 cut(s) 50, 543
MvaI CCWGG 3 cut(s) 194, 690, 1359
MvnI CGCG 1 cut(s) 534
NciI CCSGG 1 cut(s) 1310
NdeII GATC 8 cut(s) 217, 382, 670, 830, 1179, 1198, 1285, 1494
NlaIV GGNNCC 4 cut(s) 318, 1252, 1307, 1308
NmuCI GTSAC 2 cut(s) 775, 1076
NsbI TGCGCA 1 cut(s) 56
NsiI ATGCAT 1 cut(s) 187
NspI RCATGY 2 cut(s) 523, 1507
PagI TCATGA 1 cut(s) 385
PciI ACATGT 1 cut(s) 519
PdmI GAANNNNTTC 2 cut(s) 684, 989
PfeI GAWTC 2 cut(s) 307, 1204
PflFI GACNNNGTC 1 cut(s) 115
PkrI GCNGC 9 cut(s) 163, 166, 637, 850, 931, 1046, 1110, 1150, 1399
PleI GAGTC 1 cut(s) 217
PpsI GAGTC 1 cut(s) 217
PpuMI RGGWCCY 2 cut(s) 1251, 1306
PscI ACATGT 1 cut(s) 519
PshBI ATTAAT 1 cut(s) 323
PsiI TTATAA 1 cut(s) 731
Psp5II RGGWCCY 2 cut(s) 1251, 1306
Psp6I CCWGG 3 cut(s) 192, 688, 1357
PspFI CCCAGC 2 cut(s) 666, 1331
PspGI CCWGG 3 cut(s) 192, 688, 1357
PspN4I GGNNCC 4 cut(s) 318, 1252, 1307, 1308
PspPI GGNCC 2 cut(s) 1251, 1306
PspPPI RGGWCCY 2 cut(s) 1251, 1306
PstI CTGCAG 3 cut(s) 603, 1105, 1412
PstNI CAGNNNCTG 3 cut(s) 644, 848, 1364
PsuI RGATCY 2 cut(s) 1179, 1494
PsyI GACNNNGTC 1 cut(s) 115
PvuII CAGCTG 2 cut(s) 164, 1111
RsaI GTAC 1 cut(s) 597
RsaNI GTAC 1 cut(s) 596
SaqAI TTAA 5 cut(s) 323, 479, 569, 660, 879
SatI GCNGC 9 cut(s) 162, 165, 636, 849, 930, 1045, 1109, 1149, 1398
Sau3AI GATC 8 cut(s) 217, 382, 670, 830, 1179, 1198, 1285, 1494
Sau96I GGNCC 2 cut(s) 1251, 1306
SbfI CCTGCAGG 1 cut(s) 1105
SchI GAGTC 1 cut(s) 218
ScrFI CCNGG 4 cut(s) 194, 690, 1310, 1359
SdaI CCTGCAGG 1 cut(s) 1105
SduI GDGCHC 1 cut(s) 175
SfaNI GCATC 7 cut(s) 172, 194, 287, 631, 835, 1180, 1404
SfcI CTRYAG 4 cut(s) 599, 1101, 1255, 1408
SinI GGWCC 2 cut(s) 1251, 1306
SmlI CTYRAG 3 cut(s) 435, 878, 1172
SmoI CTYRAG 3 cut(s) 435, 878, 1172
Sse8387I CCTGCAGG 1 cut(s) 1105
SsiI CCGC 4 cut(s) 228, 532, 1025, 1501
SspI AATATT 1 cut(s) 40
StyD4I CCNGG 4 cut(s) 192, 688, 1308, 1357
StyI CCWWGG 3 cut(s) 312, 943, 1246
TaaI ACNGT 3 cut(s) 817, 1040, 1436
TaiI ACGT 1 cut(s) 1034
TaqI TCGA 7 cut(s) 98, 104, 110, 220, 232, 352, 1452
TfiI GAWTC 2 cut(s) 307, 1204
Tru1I TTAA 5 cut(s) 323, 479, 569, 660, 879
Tru9I TTAA 5 cut(s) 323, 479, 569, 660, 879
TscAI CASTG 3 cut(s) 364, 608, 1229
TseFI GTSAC 2 cut(s) 775, 1076
TseI GCWGC 9 cut(s) 161, 164, 635, 848, 929, 1044, 1108, 1148, 1397
Tsp45I GTSAC 2 cut(s) 775, 1076
TspDTI ATGAA 2 cut(s) 1155, 1196
TspRI CASTG 3 cut(s) 364, 608, 1229
Tth111I GACNNNGTC 1 cut(s) 115
Vha464I CTTAAG 1 cut(s) 878
VpaK11BI GGWCC 2 cut(s) 1251, 1306
VspI ATTAAT 1 cut(s) 323
XapI RAATTY 4 cut(s) 474, 582, 783, 1240
XceI RCATGY 2 cut(s) 523, 1507
XmnI GAANNNNTTC 2 cut(s) 684, 989
ZraI GACGTC 1 cut(s) 1032
Zsp2I ATGCAT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.