pycom17g12880

Pleckstrin homology domain.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012240
Physical Location & Seq
Reverse (-)
10592112 .. 10596050
3939 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1494 bp
ATGGCCACCATCAATGGCGCCTCTCCGCGAGCTGCGGACGCAGAGAACAGCTTGGAGAAGATCAAGCGCCAGCTCACATCGGGTTCGGGTCGGAACTTGTTGCAGGGTCCACTTCTCAAGCGATCCGAGACTCTGAGAAAATGGAACGAGCGGTGGATGATCTTAGACCCAACGACAGGGAGAATGGAATACAAGATTAGGAGAAATGAGCCGACTGTCAAGGGAACCATTCTATTTGATGCGAATAGCACAATTACCCTATCTCCAGTGAACTTCCATGGGTTGCAAAAGTATGACGGTTGCTGTTTCTATATTGGGACCCCGCAGAAAAAAGACTACTTCCTTTGTGCAGAGACTCCTGGGGCAGCTAGAGCTTGGGTAGCAACACTGCAAGCAACACAATTGGTTCTAAAGGCCCACAAAGAAGCTGTGAATTCCTTGAGTGGCAGTGGGTCTGCAAAACTAGGAACGGTTGCAGCAGTAGTTGCTGCTGCCAATTCAACTGCTGCGGAGAGTTCTAAGGAAATCGAAGCAGCAATGCACATTTCTTTGAGAAATGCCTTGGGAATGATAACCAATAAGCCAAATGATGGTCCTATGGATGATTTTGCAATTATGAAGGAGACACTTCGGGTCAAAGATGACGAACTACATAATTTGGCTAGGGACCTTCGTTCGCGCGATTCAACAATTAAAGAAATAGCAGAGAAATTAACTGAGACTGCTGAAGCTGCTGAGGCTGCAGCCTCTGCAGCTCATACAATAGATGAACAAAGGAAAATTGTTTGTGCAGAAATTGATCGCTTAAAAAGGGATTCAGCAAAGCAGCTGGAATCTTCGTTGTCAAAGCTAAAAGATTCTGAAGAAAAGGTTGCAACCTTGAGCAAAGAAAGAGACCAATTGACTAGGCAGAGAGATTCTGCTGTCCAAGAGGCACACATGTGGCGTTCTGAGCTTGCAAAAGCCAGAGAGCGTGTTGTGATATTAGAAGCAGCTGTTGTGAGAGCAGAGGAGAAGGTCAGGGTTACAGAGGCAGATGCTCAAGCTAGAATAAAGGAGGCCGTGCAGAAAGAATCAACTGCACTGAAAGACAAGCAAGAACTTCTGGAATATGTGAATACGTTGCAAGTGCAACTTCAAAGACATCACATTGATACAAAGCAAGTGTTTGAGGAGAAGTCTGAGTCTATTGGAAATACACCCCTGACTAAACATGTAGACTTGTCCCAAGTAAATGTTGATAAAGCATGCCTCAGTGTTTCTAGAGCAGTTCCGGTAGCAGGGGAGAGTGTAGTTCGCATGGCTGAGGGTCAAGTCAACCTACGGCCAGTAGGTGACGGTGAATGGAGTGATAATCAGGCTCAAGATGCAAGGATAGCCGATGTAAGAGAAGTCGCCCCCCAATCAGAAGCAAGTAGCTTGGATATTCCAGTTGTCAGCCAACCAACTGGTAATCATCATGAGCAAGGAGTGGACTCTTTTCATCAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PH

Protein Analysis

498

Amino Acids

54.71

Weight (kDa)

6.24

Isoelectric Point (pI)

45.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PH PF00169 34 - 132 2.4e-06 PH domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 17
AccB7I CCANNNNNTGG 1 cut(s) 590
AccBSI CCGCTC 1 cut(s) 151
AccI GTMKAC 1 cut(s) 1218
AccII CGCG 3 cut(s) 28, 679, 681
AciI CCGC 5 cut(s) 26, 35, 151, 323, 509
AclWI GGATC 1 cut(s) 117
AcoI YGGCCR 2 cut(s) 3, 1325
AcsI RAATTY 1 cut(s) 433
AcuI CTGAAG 2 cut(s) 747, 882
AcyI GRCGYC 1 cut(s) 18
AfiI CCNNNNNNNGG 3 cut(s) 176, 590, 1280
AflIII ACRYGT 2 cut(s) 939, 1213
AgsI TTSAA 3 cut(s) 501, 687, 1139
AjnI CCWGG 1 cut(s) 358
AleI CACNNNNGTG 1 cut(s) 940
Alw26I GTCTC 5 cut(s) 122, 347, 617, 713, 888
AlwI GGATC 1 cut(s) 117
AlwNI CAGNNNCTG 1 cut(s) 749
AoxI GGCC 4 cut(s) 3, 414, 1059, 1325
ApoI RAATTY 1 cut(s) 433
AspLEI GCGC 3 cut(s) 20, 69, 681
AspS9I GGNCC 5 cut(s) 107, 318, 415, 593, 667
AsuHPI GGTGA 2 cut(s) 1346, 1352
AvaII GGWCC 4 cut(s) 107, 318, 593, 667
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 1 cut(s) 17
BbvCI CCTCAGC 2 cut(s) 735, 1305
BccI CCATC 2 cut(s) 17, 584
BceAI ACGGC 2 cut(s) 1046, 1340
BciT130I CCWGG 1 cut(s) 360
BcoDI GTCTC 5 cut(s) 122, 347, 617, 713, 888
BfaI CTAG 6 cut(s) 369, 464, 663, 906, 1047, 1263
BfmI CTRYAG 2 cut(s) 741, 750
BfoI RGCGCY 2 cut(s) 21, 70
Bme1390I CCNGG 1 cut(s) 360
Bme18I GGWCC 4 cut(s) 107, 318, 593, 667
BmgT120I GGNCC 5 cut(s) 107, 318, 415, 593, 667
BmiI GGNNCC 6 cut(s) 19, 108, 226, 319, 320, 668
BmrFI CCNGG 1 cut(s) 360
BmsI GCATC 3 cut(s) 229, 1027, 1357
BpmI CTGGAG 1 cut(s) 249
Bpu10I CCTNAGC 2 cut(s) 735, 1305
BpuEI CTTGAG 5 cut(s) 101, 460, 901, 1026, 1347
BsaHI GRCGYC 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 888
BsaJI CCNNGG 3 cut(s) 277, 359, 561
BsaWI WCCGGW 1 cut(s) 1273
BsaXI ACNNNNNCTCC 2 cut(s) 247, 277
Bsc4I CCNNNNNNNGG 3 cut(s) 176, 590, 1280
Bse1I ACTGG 4 cut(s) 266, 1328, 1430, 1453
Bse3DI GCAATG 1 cut(s) 543
BseBI CCWGG 1 cut(s) 360
BseDI CCNNGG 3 cut(s) 277, 359, 561
BseGI GGATG 2 cut(s) 162, 607
BseLI CCNNNNNNNGG 3 cut(s) 176, 590, 1280
BseMI GCAATG 1 cut(s) 543
BseMII CTCAG 7 cut(s) 125, 708, 726, 942, 1173, 1267, 1296
BseNI ACTGG 4 cut(s) 266, 1328, 1430, 1453
BseRI GAGGAG 2 cut(s) 1025, 1187
BsgI GTGCAG 4 cut(s) 369, 810, 1065, 1085
Bsh1236I CGCG 3 cut(s) 28, 679, 681
BshFI GGCC 4 cut(s) 5, 416, 1061, 1327
BshNI GGYRCC 1 cut(s) 17
BsiSI CCGG 1 cut(s) 1274
BslFI GGGAC 3 cut(s) 331, 680, 1210
BslI CCNNNNNNNGG 3 cut(s) 176, 590, 1280
BsmAI GTCTC 5 cut(s) 122, 347, 617, 713, 888
BsmFI GGGAC 3 cut(s) 331, 680, 1210
BsnI GGCC 4 cut(s) 5, 416, 1061, 1327
Bso31I GGTCTC 1 cut(s) 888
Bsp143I GATC 4 cut(s) 60, 122, 159, 799
Bsp19I CCATGG 1 cut(s) 277
BspACI CCGC 5 cut(s) 26, 35, 151, 323, 509
BspANI GGCC 4 cut(s) 5, 416, 1061, 1327
BspCNI CTCAG 7 cut(s) 126, 709, 727, 943, 1174, 1266, 1297
BspFNI CGCG 3 cut(s) 28, 679, 681
BspHI TCATGA 1 cut(s) 1459
BspLI GGNNCC 6 cut(s) 19, 108, 226, 319, 320, 668
BspMAI CTGCAG 2 cut(s) 745, 754
BspPI GGATC 1 cut(s) 117
BspT107I GGYRCC 1 cut(s) 17
BspTNI GGTCTC 1 cut(s) 888
BsrBI CCGCTC 1 cut(s) 151
BsrDI GCAATG 1 cut(s) 543
BsrI ACTGG 4 cut(s) 266, 1328, 1430, 1453
BssECI CCNNGG 3 cut(s) 277, 359, 561
BssMI GATC 4 cut(s) 60, 122, 159, 799
BssNI GRCGYC 1 cut(s) 18
BssT1I CCWWGG 2 cut(s) 277, 561
Bst2UI CCWGG 1 cut(s) 360
Bst4CI ACNGT 4 cut(s) 217, 299, 472, 1340
BstACI GRCGYC 1 cut(s) 18
BstAPI GCANNNNNTGC 2 cut(s) 485, 749
BstC8I GCNNGC 5 cut(s) 30, 71, 393, 957, 1249
BstDEI CTNAG 9 cut(s) 134, 163, 519, 717, 735, 951, 1182, 1253, 1305
BstDSI CCRYGG 1 cut(s) 277
BstF5I GGATG 2 cut(s) 162, 607
BstFNI CGCG 3 cut(s) 28, 679, 681
BstH2I RGCGCY 2 cut(s) 21, 70
BstHHI GCGC 3 cut(s) 20, 69, 681
BstKTI GATC 4 cut(s) 63, 125, 162, 802
BstMAI GTCTC 5 cut(s) 122, 347, 617, 713, 888
BstMBI GATC 4 cut(s) 60, 122, 159, 799
BstNI CCWGG 1 cut(s) 360
BstNSI RCATGY 3 cut(s) 943, 1217, 1251
BstSCI CCNGG 1 cut(s) 358
BstSFI CTRYAG 2 cut(s) 741, 750
BstUI CGCG 3 cut(s) 28, 679, 681
BstXI CCANNNNNNTGG 1 cut(s) 1448
BsuRI GGCC 4 cut(s) 5, 416, 1061, 1327
BtgI CCRYGG 1 cut(s) 277
BtsCI GGATG 2 cut(s) 162, 607
BtsI GCAGTG 2 cut(s) 386, 454
BtsIMutI CAGTG 5 cut(s) 273, 386, 454, 1082, 1261
Cac8I GCNNGC 5 cut(s) 30, 71, 393, 957, 1249
CaiI CAGNNNCTG 1 cut(s) 749
CciI TCATGA 1 cut(s) 1459
CfoI GCGC 3 cut(s) 20, 69, 681
Cfr13I GGNCC 5 cut(s) 107, 318, 415, 593, 667
CseI GACGC 1 cut(s) 47
CviAII CATG 6 cut(s) 278, 940, 1214, 1248, 1300, 1460
DdeI CTNAG 9 cut(s) 134, 163, 519, 717, 735, 951, 1182, 1253, 1305
DinI GGCGCC 1 cut(s) 19
DpnI GATC 4 cut(s) 62, 124, 161, 801
DpnII GATC 4 cut(s) 60, 122, 159, 799
EaeI YGGCCR 2 cut(s) 3, 1325
Eco130I CCWWGG 2 cut(s) 277, 561
Eco31I GGTCTC 1 cut(s) 888
Eco47I GGWCC 4 cut(s) 107, 318, 593, 667
Eco57I CTGAAG 2 cut(s) 747, 882
EcoO109I RGGNCCY 2 cut(s) 318, 667
EcoRI GAATTC 1 cut(s) 433
EcoRII CCWGG 1 cut(s) 358
EcoT14I CCWWGG 2 cut(s) 277, 561
EgeI GGCGCC 1 cut(s) 19
EheI GGCGCC 1 cut(s) 19
ErhI CCWWGG 2 cut(s) 277, 561
FaeI CATG 6 cut(s) 281, 943, 1217, 1251, 1303, 1463
FalI AAGNNNNNCTT 2 cut(s) 1119, 1151
FaqI GGGAC 3 cut(s) 331, 680, 1210
FatI CATG 6 cut(s) 277, 939, 1213, 1247, 1299, 1459
FauI CCCGC 1 cut(s) 330
FblI GTMKAC 1 cut(s) 1218
FokI GGATG 2 cut(s) 169, 614
FspBI CTAG 6 cut(s) 369, 464, 663, 906, 1047, 1263
GlaI GCGC 3 cut(s) 19, 68, 680
GsuI CTGGAG 1 cut(s) 249
HaeII RGCGCY 2 cut(s) 21, 70
HaeIII GGCC 4 cut(s) 5, 416, 1061, 1327
HapII CCGG 1 cut(s) 1274
HgaI GACGC 1 cut(s) 47
HhaI GCGC 3 cut(s) 20, 69, 681
Hin1I GRCGYC 1 cut(s) 18
Hin1II CATG 6 cut(s) 281, 943, 1217, 1251, 1303, 1463
Hin6I GCGC 3 cut(s) 18, 67, 679
HinP1I GCGC 3 cut(s) 18, 67, 679
HincII GTYRAC 1 cut(s) 1318
HindII GTYRAC 1 cut(s) 1318
HpaII CCGG 1 cut(s) 1274
HphI GGTGA 2 cut(s) 1346, 1352
Hpy166II GTNNAC 5 cut(s) 110, 271, 1219, 1318, 1474
Hpy188I TCNGA 7 cut(s) 93, 127, 135, 862, 952, 1183, 1408
Hpy188III TCNNGA 4 cut(s) 1106, 1263, 1364, 1460
Hpy8I GTNNAC 5 cut(s) 110, 271, 1219, 1318, 1474
HpyAV CCTTC 3 cut(s) 613, 680, 1009
HpyCH4III ACNGT 4 cut(s) 217, 299, 472, 1340
HpyCH4IV ACGT 1 cut(s) 1121
HpyF3I CTNAG 9 cut(s) 134, 163, 519, 717, 735, 951, 1182, 1253, 1305
HpySE526I ACGT 1 cut(s) 1121
Hsp92I GRCGYC 1 cut(s) 18
Hsp92II CATG 6 cut(s) 281, 943, 1217, 1251, 1303, 1463
HspAI GCGC 3 cut(s) 18, 67, 679
KasI GGCGCC 1 cut(s) 17
KflI GGGWCCC 1 cut(s) 318
Kzo9I GATC 4 cut(s) 60, 122, 159, 799
LweI GCATC 3 cut(s) 229, 1027, 1357
MaeI CTAG 6 cut(s) 369, 464, 663, 906, 1047, 1263
MaeII ACGT 1 cut(s) 1121
MaeIII GTNAC 2 cut(s) 1024, 1334
MalI GATC 4 cut(s) 62, 124, 161, 801
MbiI CCGCTC 1 cut(s) 151
MboI GATC 4 cut(s) 60, 122, 159, 799
MboII GAAGA 3 cut(s) 70, 828, 875
MfeI CAATTG 2 cut(s) 401, 899
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 18
MlyI GAGTC 4 cut(s) 124, 349, 1193, 1469
MmeI TCCRAC 1 cut(s) 71
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 693, 713, 806
MslI CAYNNNNRTG 1 cut(s) 940
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 2 cut(s) 829, 995
MspI CCGG 1 cut(s) 1274
MspR9I CCNGG 1 cut(s) 360
MunI CAATTG 2 cut(s) 401, 899
MvaI CCWGG 1 cut(s) 360
MvnI CGCG 3 cut(s) 28, 679, 681
NarI GGCGCC 1 cut(s) 18
NcoI CCATGG 1 cut(s) 277
NdeII GATC 4 cut(s) 60, 122, 159, 799
NlaIII CATG 6 cut(s) 281, 943, 1217, 1251, 1303, 1463
NlaIV GGNNCC 6 cut(s) 19, 108, 226, 319, 320, 668
NmuCI GTSAC 1 cut(s) 1334
NspI RCATGY 3 cut(s) 943, 1217, 1251
OliI CACNNNNGTG 1 cut(s) 940
PaeI GCATGC 1 cut(s) 1251
PagI TCATGA 1 cut(s) 1459
PciI ACATGT 2 cut(s) 939, 1213
PfeI GAWTC 6 cut(s) 683, 815, 833, 857, 917, 1073
PflMI CCANNNNNTGG 1 cut(s) 590
PleI GAGTC 4 cut(s) 124, 349, 1192, 1469
PluTI GGCGCC 1 cut(s) 21
PpsI GAGTC 4 cut(s) 124, 349, 1192, 1469
PpuMI RGGWCCY 2 cut(s) 318, 667
PscI ACATGT 2 cut(s) 939, 1213
Psp5II RGGWCCY 2 cut(s) 318, 667
Psp6I CCWGG 1 cut(s) 358
PspGI CCWGG 1 cut(s) 358
PspN4I GGNNCC 6 cut(s) 19, 108, 226, 319, 320, 668
PspPI GGNCC 5 cut(s) 107, 318, 415, 593, 667
PspPPI RGGWCCY 2 cut(s) 318, 667
PstI CTGCAG 2 cut(s) 745, 754
PstNI CAGNNNCTG 1 cut(s) 749
PvuII CAGCTG 2 cut(s) 829, 995
RseI CAYNNNNRTG 1 cut(s) 940
SaqAI TTAA 3 cut(s) 693, 713, 806
Sau3AI GATC 4 cut(s) 60, 122, 159, 799
Sau96I GGNCC 5 cut(s) 107, 318, 415, 593, 667
SchI GAGTC 4 cut(s) 124, 349, 1193, 1469
ScrFI CCNGG 1 cut(s) 360
SfaNI GCATC 3 cut(s) 229, 1027, 1357
SfcI CTRYAG 2 cut(s) 741, 750
SfoI GGCGCC 1 cut(s) 19
SinI GGWCC 4 cut(s) 107, 318, 593, 667
SmiMI CAYNNNNRTG 1 cut(s) 940
SmlI CTYRAG 5 cut(s) 116, 439, 880, 1041, 1362
SmoI CTYRAG 5 cut(s) 116, 439, 880, 1041, 1362
SphI GCATGC 1 cut(s) 1251
SsiI CCGC 5 cut(s) 26, 35, 151, 323, 509
SspDI GGCGCC 1 cut(s) 17
SspMI CTAG 6 cut(s) 369, 464, 663, 906, 1047, 1263
StyD4I CCNGG 1 cut(s) 358
StyI CCWWGG 2 cut(s) 277, 561
TaaI ACNGT 4 cut(s) 217, 299, 472, 1340
TaiI ACGT 1 cut(s) 1124
TaqI TCGA 1 cut(s) 528
TfiI GAWTC 6 cut(s) 683, 815, 833, 857, 917, 1073
Tru1I TTAA 3 cut(s) 693, 713, 806
Tru9I TTAA 3 cut(s) 693, 713, 806
TscAI CASTG 5 cut(s) 273, 393, 454, 1089, 1261
TseFI GTSAC 1 cut(s) 1334
Tsp45I GTSAC 1 cut(s) 1334
TspDTI ATGAA 3 cut(s) 632, 783, 1472
TspRI CASTG 5 cut(s) 273, 393, 454, 1089, 1261
Van91I CCANNNNNTGG 1 cut(s) 590
VpaK11BI GGWCC 4 cut(s) 107, 318, 593, 667
XapI RAATTY 1 cut(s) 433
XbaI TCTAGA 1 cut(s) 1262
XceI RCATGY 3 cut(s) 943, 1217, 1251
XmiI GTMKAC 1 cut(s) 1218
XspI CTAG 6 cut(s) 369, 464, 663, 906, 1047, 1263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.