pycom17g22470

F-box LRR-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0016058
Physical Location & Seq
Reverse (-)
20836047 .. 20837804
1758 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g22470.1

Sequence Viewer

Length: 1758 bp
ATGGCAGAGAAATCTGCAGATTTGCCAGAAGAATGCTGGGAATTGATACTCAAACAGCTACTGCTAGACCTAGACCCAGACCACCCCTCTCCCTTCAATTCACTATCTCTCGTCAGCAAGCAGTTCCTCTCCTTCACCAATCGCCTTCTATCCACTCTCAAACTCAAACGCTCCGATCCAGCACTCCAATCCCTCCTCCCTCGTCTTCTCCGACGGTTCCCTCGTCTCAAACACATCGACCTCGCCAAATTCCATGGCGACATGAACCCAATTCTGCATCAAATTGCGCTATCTGGGTTGAACCTAGAATCCCTCAACGTTTCCGATCAGGCGACTTTACCCGCGGTTGGTTTAAGATCATTGGGTTCGAAATTGGAGAATTTGAAGAGTCTGAACTGCTCCAAAATCCGCTGTTTTGGGGATTCTGATCTGAATTTGATTGCAGATTCTTTTCCACGCCTTGAAGAGCTTGACATCGGCTGGCCTGATGATGAATATGGTTTCTCCCCAATTGGGGTTTCAGATTCCACCCCTCTCTCGAGGCCGATATCCGATTCGGGCATCTTATCGCTGTCGGAGAAGCTCAAGAGCCTGAGAAAGATCCACCTCTCTGGTAATAGCTTCATCACTGATAAATCGCTTGTGTTCTTGTCCACCAACTGTTTGTGTTTATGCCAAATTGTGTTGAACTTTTGTCACTTCATAACACTGAATGGCATATCTAAGCTCCTCTGTGACTGTCGCCAATTGAATTATATTTCGGCTAGTAGAACTGGGAACCTATCCTTTGATGTACCACCTCAAGTACCATTCATAAGCTCGAAAGCTTTGTTGGCTATTGATCTTTCGGGTTGGATGATTTCGGATGAATTTCTGGATGCGGTTGCAGAAGCACATTTGCCTTTAAAGCAGCTCATTCTTTCTGGATGTACAAACTACAGTTTTGCTGGAATTCGGGTTTTGTTGAGTGAGTACCAGTCCCTTGAGTACTTAGATCTTGAGTCGGCACATTTTCTCAGAGACAAGGACATGGCCGATCTATCCCTGTTCCTCCATAGTATCAGATATATTAACCTTAATTATTGCAGTAAGGTGACCGATATCACCTTCTATATCCTCATAGCAAGCTGTCTGGTTTTGAATACAATGGAAATGGCAGGAACAGGTATTGGGGACGGAGACATTGAGACGGATTGTAAGGCAAACAATCAAATGAAATCGCTGAACTTGGCTCAAAATAGCTCAATAGACGATGCCTTTTTGACAAGTTTCGCATCTATTTTCCCCAATCTGGAAGTGCTTGATTTGAGCGGCTGTAAGGGAGTATCAGAAGAAGGCATTGTAGAAGTACTAAAGAGATGCTCTGAGATTAGGGAATTGAAGATAGATGGTAGAGTGAGGAAGAATCTTTGTTTACTCCTTGAGTTTCAACTTCCGAAGTTGACGGTATTGAAATGGATGCGTTCGGGAATCGATGATGAGGCACTGGCTATGATTGGGAAAAGATGTCCCAGCCTACAGAAATTGAACTTGTTGGGCAGCGGGAGTTTGACATCAGAAGGGGTGAAGGGACTGGTGAAGAACTGCAAAGGGCTGAGGGAGATAAATTTGAGGTCTTGTAATAATGTCAGTGCTGATATTGTTTCGTGGATGGTTTTCTCTTCGTCGCTGAAGAAAATAGTTCCGCCTCCTGGTTATGTTCATACCGAAACCCAAAGGAACTTTTTCTTGCTTCATGGGTGCCTTGTTTGTGAGTGA

Protein Analysis

586

Amino Acids

64.91

Weight (kDa)

5.95

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7885 PF25372 354 - 461 4.4e-06 Leucine Rich Repeat Domain of unknown function (DUF7885)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016058)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G80630
fragaria_vesca FvH4_6g28940
prunus_persica Prupe.3G095400_v2.0.a1 Prupe.3G095400_v2.0.a1
pyrus_communis pycom17g22470
rosa_chinensis RchiOBHm_Chr2g0133501
rosa_laevigata RLG00000019348
rosa_multiflora Rmu_co8455141.1_g000001 Rmu_sc0017323.1_g000002
rosa_roxburghii Rroxscaffold_2G00111020
rosa_rugosa Rorug02G0312400
rosa_samantha Rh2AG365400 Rh2BG370900 Rh2CG348500 Rh2DG387700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1740
AccBSI CCGCTC 1 cut(s) 1311
AccII CGCG 1 cut(s) 344
AciI CCGC 7 cut(s) 342, 344, 409, 881, 1311, 1542, 1685
AclI AACGTT 1 cut(s) 318
AclWI GGATC 2 cut(s) 170, 595
AcoI YGGCCR 1 cut(s) 1032
AcsI RAATTY 6 cut(s) 248, 379, 433, 869, 951, 1606
AcuI CTGAAG 1 cut(s) 1691
AfaI GTAC 6 cut(s) 795, 807, 931, 974, 989, 1350
AfiI CCNNNNNNNGG 5 cut(s) 347, 513, 514, 1291, 1691
AjnI CCWGG 1 cut(s) 1690
AjuI GAANNNNNNNTTGG 4 cut(s) 502, 534, 815, 847
AloI GAACNNNNNNTCC 2 cut(s) 293, 325
Alw26I GTCTC 4 cut(s) 230, 1014, 1173, 1181
AlwI GGATC 2 cut(s) 170, 595
AlwNI CAGNNNCTG 1 cut(s) 61
Ama87I CYCGRG 1 cut(s) 538
AoxI GGCC 3 cut(s) 482, 542, 1032
ApeKI GCWGC 2 cut(s) 910, 1539
ApoI RAATTY 6 cut(s) 248, 379, 433, 869, 951, 1606
Asp700I GAANNNNTTC 1 cut(s) 1724
AspLEI GCGC 1 cut(s) 289
AsuHPI GGTGA 5 cut(s) 127, 1096, 1105, 1576, 1588
AsuII TTCGAA 1 cut(s) 368
AvaI CYCGRG 1 cut(s) 538
BanI GGYRCC 1 cut(s) 1740
BbsI GAAGAC 1 cut(s) 197
BbvCI CCTCAGC 1 cut(s) 1595
BbvI GCAGC 2 cut(s) 922, 1551
BccI CCATC 2 cut(s) 1382, 1645
BciT130I CCWGG 1 cut(s) 1692
BcoDI GTCTC 4 cut(s) 230, 1014, 1173, 1181
BfaI CTAG 4 cut(s) 65, 71, 305, 765
BfmI CTRYAG 3 cut(s) 15, 937, 1517
BglII AGATCT 1 cut(s) 994
BisI GCNGC 3 cut(s) 911, 1312, 1540
BlsI GCNGC 3 cut(s) 912, 1313, 1541
BmcAI AGTACT 2 cut(s) 989, 1350
Bme1390I CCNGG 1 cut(s) 1692
BmeT110I CYCGRG 1 cut(s) 538
BmiI GGNNCC 3 cut(s) 218, 779, 1742
BmrFI CCNGG 1 cut(s) 1692
BmrI ACTGGG 1 cut(s) 783
BmsI GCATC 7 cut(s) 286, 570, 868, 1243, 1283, 1349, 1449
BmuI ACTGGG 1 cut(s) 783
BpiI GAAGAC 1 cut(s) 197
Bpu10I CCTNAGC 1 cut(s) 1595
Bpu14I TTCGAA 1 cut(s) 368
BpuEI CTTGAG 5 cut(s) 569, 786, 1004, 1019, 1442
Bsa29I ATCGAT 1 cut(s) 1473
BsaBI GATNNNNATC 1 cut(s) 426
BsaJI CCNNGG 2 cut(s) 253, 342
Bsc4I CCNNNNNNNGG 5 cut(s) 347, 513, 514, 1291, 1691
Bse1I ACTGG 4 cut(s) 778, 976, 1491, 1578
Bse8I GATNNNNATC 1 cut(s) 426
BseBI CCWGG 1 cut(s) 1692
BseCI ATCGAT 1 cut(s) 1473
BseDI CCNNGG 2 cut(s) 253, 342
BseGI GGATG 6 cut(s) 861, 871, 883, 932, 1464, 1656
BseJI GATNNNNATC 1 cut(s) 426
BseLI CCNNNNNNNGG 5 cut(s) 347, 513, 514, 1291, 1691
BseMII CTCAG 4 cut(s) 584, 1030, 1356, 1586
BseNI ACTGG 4 cut(s) 778, 976, 1491, 1578
BseRI GAGGAG 2 cut(s) 185, 719
BseXI GCAGC 2 cut(s) 922, 1551
BseYI CCCAGC 2 cut(s) 36, 1511
Bsh1236I CGCG 1 cut(s) 344
BshFI GGCC 3 cut(s) 484, 544, 1034
BshNI GGYRCC 1 cut(s) 1740
BshVI ATCGAT 1 cut(s) 1473
BsiHKCI CYCGRG 1 cut(s) 538
BslFI GGGAC 4 cut(s) 964, 1187, 1494, 1584
BslI CCNNNNNNNGG 5 cut(s) 347, 513, 514, 1291, 1691
BsmAI GTCTC 4 cut(s) 230, 1014, 1173, 1181
BsmBI CGTCTC 2 cut(s) 230, 1181
BsmFI GGGAC 4 cut(s) 964, 1187, 1494, 1584
BsmI GAATGC 1 cut(s) 38
BsnI GGCC 3 cut(s) 484, 544, 1034
BsoBI CYCGRG 1 cut(s) 538
Bsp119I TTCGAA 1 cut(s) 368
Bsp1407I TGTACA 1 cut(s) 929
Bsp143I GATC 8 cut(s) 175, 325, 356, 427, 600, 841, 994, 1036
Bsp19I CCATGG 1 cut(s) 253
BspACI CCGC 7 cut(s) 342, 344, 409, 881, 1311, 1542, 1685
BspANI GGCC 3 cut(s) 484, 544, 1034
BspCNI CTCAG 4 cut(s) 585, 1029, 1357, 1587
BspDI ATCGAT 1 cut(s) 1473
BspFNI CGCG 1 cut(s) 344
BspLI GGNNCC 3 cut(s) 218, 779, 1742
BspMAI CTGCAG 1 cut(s) 19
BspPI GGATC 2 cut(s) 170, 595
BspQI GCTCTTC 1 cut(s) 459
BspT104I TTCGAA 1 cut(s) 368
BspT107I GGYRCC 1 cut(s) 1740
BsrBI CCGCTC 1 cut(s) 1311
BsrGI TGTACA 1 cut(s) 929
BsrI ACTGG 4 cut(s) 778, 976, 1491, 1578
BssECI CCNNGG 2 cut(s) 253, 342
BssMI GATC 8 cut(s) 175, 325, 356, 427, 600, 841, 994, 1036
BssT1I CCWWGG 1 cut(s) 253
Bst2UI CCWGG 1 cut(s) 1692
Bst4CI ACNGT 5 cut(s) 216, 662, 740, 941, 1447
Bst6I CTCTTC 3 cut(s) 380, 459, 1666
BstAUI TGTACA 1 cut(s) 929
BstBI TTCGAA 1 cut(s) 368
BstC8I GCNNGC 3 cut(s) 119, 482, 1126
BstDEI CTNAG 6 cut(s) 593, 723, 991, 1016, 1365, 1595
BstDSI CCRYGG 2 cut(s) 253, 342
BstEII GGTNACC 1 cut(s) 1093
BstF5I GGATG 6 cut(s) 861, 871, 883, 932, 1464, 1656
BstFNI CGCG 1 cut(s) 344
BstHHI GCGC 1 cut(s) 289
BstKTI GATC 8 cut(s) 178, 328, 359, 430, 603, 844, 997, 1039
BstMAI GTCTC 4 cut(s) 230, 1014, 1173, 1181
BstMBI GATC 8 cut(s) 175, 325, 356, 427, 600, 841, 994, 1036
BstMWI GCNNNNNNNGC 2 cut(s) 833, 907
BstNI CCWGG 1 cut(s) 1692
BstPI GGTNACC 1 cut(s) 1093
BstSCI CCNGG 1 cut(s) 1690
BstSFI CTRYAG 3 cut(s) 15, 937, 1517
BstUI CGCG 1 cut(s) 344
BstV1I GCAGC 2 cut(s) 922, 1551
BstV2I GAAGAC 1 cut(s) 197
BstX2I RGATCY 2 cut(s) 600, 994
BstXI CCANNNNNNTGG 1 cut(s) 611
BstYI RGATCY 2 cut(s) 600, 994
Bsu15I ATCGAT 1 cut(s) 1473
BsuRI GGCC 3 cut(s) 484, 544, 1034
BsuTUI ATCGAT 1 cut(s) 1473
BtgI CCRYGG 2 cut(s) 253, 342
BtsCI GGATG 6 cut(s) 861, 871, 883, 932, 1464, 1656
BtsIMutI CAGTG 4 cut(s) 627, 707, 1484, 1636
Cac8I GCNNGC 3 cut(s) 119, 482, 1126
CaiI CAGNNNCTG 1 cut(s) 61
CfoI GCGC 1 cut(s) 289
Cfr42I CCGCGG 1 cut(s) 345
ClaI ATCGAT 1 cut(s) 1473
Csp6I GTAC 6 cut(s) 794, 806, 930, 973, 988, 1349
CviAII CATG 4 cut(s) 254, 262, 1030, 1736
CviQI GTAC 6 cut(s) 794, 806, 930, 973, 988, 1349
DdeI CTNAG 6 cut(s) 593, 723, 991, 1016, 1365, 1595
DpnI GATC 8 cut(s) 177, 327, 358, 429, 602, 843, 996, 1038
DpnII GATC 8 cut(s) 175, 325, 356, 427, 600, 841, 994, 1036
DraI TTTAAA 1 cut(s) 906
EaeI YGGCCR 1 cut(s) 1032
Eam1104I CTCTTC 3 cut(s) 380, 459, 1666
EarI CTCTTC 3 cut(s) 380, 459, 1666
EciI GGCGGA 1 cut(s) 1674
Eco130I CCWWGG 1 cut(s) 253
Eco32I GATATC 2 cut(s) 549, 1102
Eco57I CTGAAG 1 cut(s) 1691
Eco88I CYCGRG 1 cut(s) 538
Eco91I GGTNACC 1 cut(s) 1093
EcoO65I GGTNACC 1 cut(s) 1093
EcoRI GAATTC 1 cut(s) 951
EcoRII CCWGG 1 cut(s) 1690
EcoRV GATATC 2 cut(s) 549, 1102
EcoT14I CCWWGG 1 cut(s) 253
ErhI CCWWGG 1 cut(s) 253
Esp3I CGTCTC 2 cut(s) 230, 1181
FaeI CATG 4 cut(s) 257, 265, 1033, 1739
FaqI GGGAC 4 cut(s) 964, 1187, 1494, 1584
FatI CATG 4 cut(s) 253, 261, 1029, 1735
FauI CCCGC 2 cut(s) 349, 1535
Fnu4HI GCNGC 3 cut(s) 911, 1312, 1540
FokI GGATG 6 cut(s) 868, 878, 890, 939, 1471, 1663
Fsp4HI GCNGC 3 cut(s) 911, 1312, 1540
FspBI CTAG 4 cut(s) 65, 71, 305, 765
GlaI GCGC 1 cut(s) 288
GluI GCNGC 3 cut(s) 911, 1312, 1540
GsaI CCCAGC 2 cut(s) 40, 1515
HaeIII GGCC 3 cut(s) 484, 544, 1034
HhaI GCGC 1 cut(s) 289
Hin1II CATG 4 cut(s) 257, 265, 1033, 1739
Hin6I GCGC 1 cut(s) 287
HinP1I GCGC 1 cut(s) 287
HincII GTYRAC 1 cut(s) 1443
HindII GTYRAC 1 cut(s) 1443
HindIII AAGCTT 1 cut(s) 825
HinfI GANTC 9 cut(s) 308, 388, 422, 446, 524, 554, 1001, 1405, 1470
HphI GGTGA 5 cut(s) 127, 1096, 1105, 1576, 1588
Hpy166II GTNNAC 3 cut(s) 654, 1415, 1443
Hpy188III TCNNGA 7 cut(s) 538, 586, 875, 924, 998, 1292, 1467
Hpy8I GTNNAC 3 cut(s) 654, 1415, 1443
Hpy99I CGWCG 2 cut(s) 216, 1669
HpyAV CCTTC 7 cut(s) 103, 142, 155, 1117, 1328, 1553, 1561
HpyCH4III ACNGT 5 cut(s) 216, 662, 740, 941, 1447
HpyCH4IV ACGT 1 cut(s) 318
HpyCH4V TGCA 6 cut(s) 17, 277, 443, 887, 1086, 1587
HpyF10VI GCNNNNNNNGC 2 cut(s) 833, 907
HpyF3I CTNAG 6 cut(s) 593, 723, 991, 1016, 1365, 1595
HpySE526I ACGT 1 cut(s) 318
Hsp92II CATG 4 cut(s) 257, 265, 1033, 1739
HspAI GCGC 1 cut(s) 287
KspI CCGCGG 1 cut(s) 345
Kzo9I GATC 8 cut(s) 175, 325, 356, 427, 600, 841, 994, 1036
LguI GCTCTTC 1 cut(s) 459
LmnI GCTCC 3 cut(s) 176, 404, 732
Lsp1109I GCAGC 2 cut(s) 922, 1551
LweI GCATC 7 cut(s) 286, 570, 868, 1243, 1283, 1349, 1449
MaeI CTAG 4 cut(s) 65, 71, 305, 765
MaeII ACGT 1 cut(s) 318
MaeIII GTNAC 3 cut(s) 695, 734, 1093
MalI GATC 8 cut(s) 177, 327, 358, 429, 602, 843, 996, 1038
MbiI CCGCTC 1 cut(s) 1311
MboI GATC 8 cut(s) 175, 325, 356, 427, 600, 841, 994, 1036
MfeI CAATTG 2 cut(s) 510, 746
MflI RGATCY 2 cut(s) 600, 994
MlyI GAGTC 2 cut(s) 397, 1010
MmeI TCCRAC 3 cut(s) 235, 555, 833
MroXI GAANNNNTTC 1 cut(s) 1724
MseI TTAA 4 cut(s) 353, 905, 1071, 1077
MspA1I CMGCKG 3 cut(s) 344, 411, 1542
MspR9I CCNGG 1 cut(s) 1692
MunI CAATTG 2 cut(s) 510, 746
Mva1269I GAATGC 1 cut(s) 38
MvaI CCWGG 1 cut(s) 1692
MvnI CGCG 1 cut(s) 344
MwoI GCNNNNNNNGC 2 cut(s) 833, 907
NcoI CCATGG 1 cut(s) 253
NdeII GATC 8 cut(s) 175, 325, 356, 427, 600, 841, 994, 1036
NlaIII CATG 4 cut(s) 257, 265, 1033, 1739
NlaIV GGNNCC 3 cut(s) 218, 779, 1742
NmuCI GTSAC 3 cut(s) 695, 734, 1093
NspV TTCGAA 1 cut(s) 368
PaeR7I CTCGAG 1 cut(s) 538
PciSI GCTCTTC 1 cut(s) 459
PcsI WCGNNNNNNNCGW 2 cut(s) 208, 220
PctI GAATGC 1 cut(s) 38
PdmI GAANNNNTTC 1 cut(s) 1724
PfeI GAWTC 7 cut(s) 308, 422, 446, 524, 554, 1405, 1470
PkrI GCNGC 3 cut(s) 912, 1313, 1541
PleI GAGTC 2 cut(s) 396, 1009
PpsI GAGTC 2 cut(s) 396, 1009
Psp1406I AACGTT 1 cut(s) 318
Psp6I CCWGG 1 cut(s) 1690
PspEI GGTNACC 1 cut(s) 1093
PspFI CCCAGC 2 cut(s) 36, 1511
PspGI CCWGG 1 cut(s) 1690
PspN4I GGNNCC 3 cut(s) 218, 779, 1742
PstI CTGCAG 1 cut(s) 19
PstNI CAGNNNCTG 1 cut(s) 61
PsuI RGATCY 2 cut(s) 600, 994
RsaI GTAC 6 cut(s) 795, 807, 931, 974, 989, 1350
RsaNI GTAC 6 cut(s) 794, 806, 930, 973, 988, 1349
SacII CCGCGG 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 459
SaqAI TTAA 4 cut(s) 353, 905, 1071, 1077
SatI GCNGC 3 cut(s) 911, 1312, 1540
Sau3AI GATC 8 cut(s) 175, 325, 356, 427, 600, 841, 994, 1036
ScaI AGTACT 2 cut(s) 989, 1350
SchI GAGTC 2 cut(s) 397, 1010
ScrFI CCNGG 1 cut(s) 1692
SfaNI GCATC 7 cut(s) 286, 570, 868, 1243, 1283, 1349, 1449
SfcI CTRYAG 3 cut(s) 15, 937, 1517
Sfr274I CTCGAG 1 cut(s) 538
Sfr303I CCGCGG 1 cut(s) 345
SfuI TTCGAA 1 cut(s) 368
SgrBI CCGCGG 1 cut(s) 345
SlaI CTCGAG 1 cut(s) 538
SmlI CTYRAG 6 cut(s) 538, 584, 801, 983, 998, 1421
SmoI CTYRAG 6 cut(s) 538, 584, 801, 983, 998, 1421
SsiI CCGC 7 cut(s) 342, 344, 409, 881, 1311, 1542, 1685
SspMI CTAG 4 cut(s) 65, 71, 305, 765
StyD4I CCNGG 1 cut(s) 1690
StyI CCWWGG 1 cut(s) 253
TaaI ACNGT 5 cut(s) 216, 662, 740, 941, 1447
TaiI ACGT 1 cut(s) 321
TaqI TCGA 5 cut(s) 237, 368, 539, 821, 1473
TaqII GACCGA 1 cut(s) 1112
TatI WGTACW 3 cut(s) 929, 987, 1348
TauI GCSGC 1 cut(s) 1314
TfiI GAWTC 7 cut(s) 308, 422, 446, 524, 554, 1405, 1470
Tru1I TTAA 4 cut(s) 353, 905, 1071, 1077
Tru9I TTAA 4 cut(s) 353, 905, 1071, 1077
TscAI CASTG 4 cut(s) 634, 714, 1491, 1636
TseFI GTSAC 3 cut(s) 695, 734, 1093
TseI GCWGC 2 cut(s) 910, 1539
Tsp45I GTSAC 3 cut(s) 695, 734, 1093
TspDTI ATGAA 9 cut(s) 278, 507, 613, 691, 802, 882, 1229, 1691, 1724
TspGWI ACGGA 2 cut(s) 1191, 1205
TspRI CASTG 4 cut(s) 634, 714, 1491, 1636
XapI RAATTY 6 cut(s) 248, 379, 433, 869, 951, 1606
XcmI CCANNNNNNNNNTGG 1 cut(s) 33
XhoI CTCGAG 1 cut(s) 538
XmnI GAANNNNTTC 1 cut(s) 1724
XspI CTAG 4 cut(s) 65, 71, 305, 765
ZrmI AGTACT 2 cut(s) 989, 1350
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.