pycom17g27740

Glycogen synthase kinase-3 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0017873
Physical Location & Seq
Forward (+)
25848540 .. 25849159
620 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g27740.4

Sequence Viewer

Length: 294 bp
ATGGCTTCCGTAAGTGTGGTTCCTGCTTCCGGTCTAAGGGAGCCCGGTGGAAATGCAGTCAGTGTCGATAATTTGCCAAATGAGATGAATGACATGAAAATTAGGGATGACAAGGAAATGGGGGCAACTGTTGTTGATGGTAATGGAACGGAGGCGGGTCATATTATTGTCACAACTATTGGTGGAAAGAATGGCCAGCCAAAACAGACAATAAGCTATATGGCTGAGCGCGTTGTTGGACATGGATCTTTCGGATTGGTTTTCCAGCCAGGCCAAGTGCTTAGAAACTGGTGA

Protein Analysis

98

Amino Acids

10.21

Weight (kDa)

5.53

Isoelectric Point (pI)

27.76

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0017873)

Species Orthologous Gene IDs
pyrus_communis pycom17g27740
rosa_chinensis RchiOBHm_Chr1g0334701
rosa_multiflora Rmu_sc0006031.1_g000025
rosa_roxburghii Rroxscaffold_3G00236040 Rroxscaffold_4G00316700
rosa_samantha Rh1AG140200 Rh1BG107300 Rh1CG132100 Rh1DG144100 Rh3AG277600 Rh3BG312900

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 231
AciI CCGC 1 cut(s) 155
AclWI GGATC 1 cut(s) 253
AcoI YGGCCR 1 cut(s) 193
AfiI CCNNNNNNNGG 2 cut(s) 29, 36
AjnI CCWGG 1 cut(s) 268
AluBI AGCT 1 cut(s) 216
AluI AGCT 1 cut(s) 216
AlwI GGATC 1 cut(s) 253
AoxI GGCC 2 cut(s) 193, 271
AspLEI GCGC 1 cut(s) 231
AsuC2I CCSGG 1 cut(s) 45
BalI TGGCCA 1 cut(s) 195
BanII GRGCYC 1 cut(s) 45
BccI CCATC 1 cut(s) 131
BciT130I CCWGG 1 cut(s) 270
BcnI CCSGG 1 cut(s) 45
BlpI GCTNAGC 1 cut(s) 225
Bme1390I CCNGG 2 cut(s) 45, 270
BmiI GGNNCC 2 cut(s) 21, 42
BmrFI CCNGG 2 cut(s) 45, 270
Bpu1102I GCTNAGC 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 45
BsaWI WCCGGW 1 cut(s) 29
Bsc4I CCNNNNNNNGG 2 cut(s) 29, 36
Bse1I ACTGG 1 cut(s) 293
BseBI CCWGG 1 cut(s) 270
BseGI GGATG 1 cut(s) 112
BseLI CCNNNNNNNGG 2 cut(s) 29, 36
BseMII CTCAG 1 cut(s) 216
BseNI ACTGG 1 cut(s) 293
Bsh1236I CGCG 1 cut(s) 231
BshFI GGCC 2 cut(s) 195, 273
BsiSI CCGG 2 cut(s) 30, 45
BslI CCNNNNNNNGG 2 cut(s) 29, 36
BsnI GGCC 2 cut(s) 195, 273
Bsp1286I GDGCHC 1 cut(s) 45
Bsp143I GATC 1 cut(s) 245
Bsp1720I GCTNAGC 1 cut(s) 225
BspACI CCGC 1 cut(s) 155
BspANI GGCC 2 cut(s) 195, 273
BspCNI CTCAG 1 cut(s) 217
BspFNI CGCG 1 cut(s) 231
BspLI GGNNCC 2 cut(s) 21, 42
BspPI GGATC 1 cut(s) 253
BsrI ACTGG 1 cut(s) 293
BssMI GATC 1 cut(s) 245
Bst2UI CCWGG 1 cut(s) 270
Bst4CI ACNGT 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 197
BstDEI CTNAG 3 cut(s) 35, 225, 281
BstF5I GGATG 1 cut(s) 112
BstFNI CGCG 1 cut(s) 231
BstHHI GCGC 1 cut(s) 231
BstKTI GATC 1 cut(s) 248
BstMBI GATC 1 cut(s) 245
BstNI CCWGG 1 cut(s) 270
BstSCI CCNGG 2 cut(s) 43, 268
BstUI CGCG 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 245
BstYI RGATCY 1 cut(s) 245
BsuRI GGCC 2 cut(s) 195, 273
BtsCI GGATG 1 cut(s) 112
BtsIMutI CAGTG 1 cut(s) 67
Cac8I GCNNGC 1 cut(s) 197
CfoI GCGC 1 cut(s) 231
CviAII CATG 2 cut(s) 94, 242
CviJI RGCY 8 cut(s) 5, 43, 195, 199, 216, 224, 268, 273
CviKI_1 RGCY 8 cut(s) 5, 43, 195, 199, 216, 224, 268, 273
DdeI CTNAG 3 cut(s) 35, 225, 281
DpnI GATC 1 cut(s) 247
DpnII GATC 1 cut(s) 245
EaeI YGGCCR 1 cut(s) 193
Eco24I GRGCYC 1 cut(s) 45
EcoRII CCWGG 1 cut(s) 268
EcoT38I GRGCYC 1 cut(s) 45
FaeI CATG 2 cut(s) 97, 245
FaiI YATR 5 cut(s) 95, 162, 219, 221, 243
FatI CATG 2 cut(s) 93, 241
FauI CCCGC 1 cut(s) 148
FokI GGATG 1 cut(s) 119
FriOI GRGCYC 1 cut(s) 45
GlaI GCGC 1 cut(s) 230
HaeIII GGCC 2 cut(s) 195, 273
HapII CCGG 2 cut(s) 30, 45
HhaI GCGC 1 cut(s) 231
Hin1II CATG 2 cut(s) 97, 245
Hin6I GCGC 1 cut(s) 229
HinP1I GCGC 1 cut(s) 229
HpaII CCGG 2 cut(s) 30, 45
Hpy188I TCNGA 1 cut(s) 254
HpyCH4III ACNGT 1 cut(s) 130
HpyCH4V TGCA 1 cut(s) 56
HpyF3I CTNAG 3 cut(s) 35, 225, 281
Hsp92II CATG 2 cut(s) 97, 245
HspAI GCGC 1 cut(s) 229
Kzo9I GATC 1 cut(s) 245
LmnI GCTCC 1 cut(s) 40
LpnPI CCDG 8 cut(s) 36, 43, 58, 209, 255, 274, 278, 282
MaeIII GTNAC 1 cut(s) 169
MalI GATC 1 cut(s) 247
MboI GATC 1 cut(s) 245
MflI RGATCY 1 cut(s) 245
MhlI GDGCHC 1 cut(s) 45
MlsI TGGCCA 1 cut(s) 195
MluCI AATT 2 cut(s) 70, 99
MluNI TGGCCA 1 cut(s) 195
MmeI TCCRAC 1 cut(s) 217
MnlI CCTC 1 cut(s) 145
Mox20I TGGCCA 1 cut(s) 195
MscI TGGCCA 1 cut(s) 195
Msp20I TGGCCA 1 cut(s) 195
MspI CCGG 2 cut(s) 30, 45
MspR9I CCNGG 2 cut(s) 45, 270
MvaI CCWGG 1 cut(s) 270
MvnI CGCG 1 cut(s) 231
NciI CCSGG 1 cut(s) 45
NdeII GATC 1 cut(s) 245
NlaIII CATG 2 cut(s) 97, 245
NlaIV GGNNCC 2 cut(s) 21, 42
NmuCI GTSAC 1 cut(s) 169
Psp6I CCWGG 1 cut(s) 268
PspGI CCWGG 1 cut(s) 268
PspN4I GGNNCC 2 cut(s) 21, 42
PsrI GAACNNNNNNTAC 1 cut(s) 35
PsuI RGATCY 1 cut(s) 245
Sau3AI GATC 1 cut(s) 245
ScrFI CCNGG 2 cut(s) 45, 270
SduI GDGCHC 1 cut(s) 45
SetI ASST 1 cut(s) 218
Sse9I AATT 2 cut(s) 70, 99
SsiI CCGC 1 cut(s) 155
StyD4I CCNGG 2 cut(s) 43, 268
TaaI ACNGT 1 cut(s) 130
TaqI TCGA 1 cut(s) 66
TasI AATT 2 cut(s) 70, 99
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 1 cut(s) 169
Tsp45I GTSAC 1 cut(s) 169
TspDTI ATGAA 2 cut(s) 101, 110
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 1 cut(s) 67
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.