pycom17g27760

Polygalacturonase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012763
Physical Location & Seq
Forward (+)
25853320 .. 25855160
1841 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1356 bp
ATGAAGAATTGCAAGTTGTCACTGCTTGTTTTCTGCATTTCTTTCTTTTGCTTCTTATTAGTGTCCACCCAAGCCAGATGGCATAACCATACAAAACACAAGCACTCTCACAAGCAATCCCACATTTCACAACCTCCTTCCTCTGCACCTGAGCCGGCTAGTCGTCCCGAAGAGCCGCCTACCAATCCGGGCCATTCCTCTGATGTTTTCGATGTAAGATCATTTGGTGCTGTAGGAGATGGCCAAACTGATGACACCGCGGCGTTTAAGATGGCATGGGACACAGCCTGCCAAAGTGATGAATCTGCCCCAAGGATCCTTGTTCCCCAAGGTTTCTCGTTCATGATACAATCTACAATATTCACTGGTCCCTGCCTTGCGGGCTTGGTGTTACAGGTTGATGGAACTCTAGTGCCACCTGATGGACCAGAGTCCTGGCCAAAGAACATCAGCAGGCACCAATGGCTGGTCTTTTACCGAGTCAACGAGATGTCGCTGCAAGGCGGCGGTGTAATTGATGGGAGAGGACAGAAATGGTGGAATCTTCCCTGCAAACCCCATAGGGCCCTGAGGTTCTTCATGAGCTCAAACTTGACAGTTCAAGGACTTAGAATTAAGAATAGCCCCCAATTCCACTTTAGATTTGATAATTGTAAAAATGTGCATATAGAATCCATTTCTATATCCGCCCCTGCAAAAAGTCCGAATACAGATGGGATTCACATAGAGAACACAAATGATGTCCAAATTTATAACTCAGTCATTTCTAATGGTGATGATTGTATATCAATTGGATCAGGCTGTTATGATGTAGACATACGGAACATCACTTGTGGACCTGGTCATGGAATCAGCATTGGGAGTCTCGGAAATCACAATTCGCTGGCATGCGTCTCTAACATTACAGTTAGGGACTCGATAATCAAAGGGACGGATAATGGAGTTAGGATCGAGACATGGCAGGGCGGGTCTGGATCGGTATCGGCAGTAACATTCAGTAATATTCACATGGATAATGTTAGAAACCCTATTATGATTGACCAATACTATTGCCTTACCAAAGGGTGCACAAACCAAACTTCAGCAGTGTTCGTATCGGACATACTCTATTCAAACATAAAGGGGACTTATGACGTTCGAAGTGCACCAATGCATTTTGCCTGCAGTGATGCTCTCCCATGCACTAACTTGACACTCTCAGAAGTTGAGCTTCTTCCTGCGGTAGGAGATATGGTATCCGACCCGTATTGTTGGAATGCTTATGGAGAGCTGCAGACGCTAACAATTCCACCAGTCTCTTGCTTGTTGGATGGTGTTCCTCGATCGCTACTGGACACTTACGTTGAGCGTTGCTGA

Protein Analysis

452

Amino Acids

49.43

Weight (kDa)

6.03

Isoelectric Point (pI)

58.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 101 - 404 7.6e-92 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012763)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G02460
fragaria_vesca FvH4_1g26650
malus_domestica MD09G1287300.v1.1 MD17G1279500.v1.1
prunus_persica Prupe.3G005900_v2.0.a1 Prupe.6G307200_v2.0.a1
pyrus_communis pycom09g19460 pycom17g27760
rosa_chinensis RchiOBHm_Chr3g0486841
rosa_laevigata RLG00000023055
rosa_multiflora Rmu_sc0005709.1_g000005
rosa_roxburghii Rroxscaffold_6G00395520
rosa_rugosa Rorug03G0227400.1
rosa_samantha Rh3AG277500 Rh3BG312600 Rh3CG311500 Rh3DG308100
rosa_wichuraiana Rw3G024640

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 753
AccB1I GGYRCC 1 cut(s) 456
AccB7I CCANNNNNTGG 2 cut(s) 422, 466
AccI GTMKAC 1 cut(s) 813
AccII CGCG 1 cut(s) 260
AciI CCGC 9 cut(s) 176, 258, 260, 380, 504, 507, 687, 966, 1220
AclWI GGATC 5 cut(s) 310, 323, 802, 956, 982
AcoI YGGCCR 2 cut(s) 241, 437
AcsI RAATTY 1 cut(s) 747
AcuI CTGAAG 1 cut(s) 1065
AfiI CCNNNNNNNGG 4 cut(s) 422, 466, 845, 1223
AgsI TTSAA 2 cut(s) 602, 1113
AjnI CCWGG 2 cut(s) 434, 838
AluBI AGCT 3 cut(s) 585, 1210, 1270
AluI AGCT 3 cut(s) 585, 1210, 1270
Alw21I GWGCWC 3 cut(s) 587, 1070, 1147
Alw26I GTCTC 4 cut(s) 869, 898, 947, 1300
Alw44I GTGCAC 2 cut(s) 1066, 1143
AlwI GGATC 5 cut(s) 310, 323, 802, 956, 982
AoxI GGCC 4 cut(s) 190, 241, 437, 564
ApaI GGGCCC 1 cut(s) 568
ApaLI GTGCAC 2 cut(s) 1066, 1143
ApeKI GCWGC 2 cut(s) 496, 1270
ApoI RAATTY 1 cut(s) 747
AspS9I GGNCC 6 cut(s) 190, 368, 425, 564, 565, 836
AsuC2I CCSGG 1 cut(s) 189
AsuHPI GGTGA 1 cut(s) 785
AsuII TTCGAA 1 cut(s) 1138
AvaII GGWCC 3 cut(s) 368, 425, 836
AxyI CCTNAGG 1 cut(s) 569
BaeGI GKGCMC 3 cut(s) 568, 1070, 1147
BalI TGGCCA 2 cut(s) 243, 439
BamHI GGATCC 1 cut(s) 315
BanI GGYRCC 1 cut(s) 456
BanII GRGCYC 2 cut(s) 568, 587
Bbv12I GWGCWC 3 cut(s) 587, 1070, 1147
BbvI GCAGC 2 cut(s) 483, 1257
BccI CCATC 8 cut(s) 72, 233, 265, 395, 416, 512, 707, 1304
BciT130I CCWGG 2 cut(s) 436, 840
BciVI GTATCC 1 cut(s) 1246
BcnI CCSGG 1 cut(s) 189
BcoDI GTCTC 4 cut(s) 869, 898, 947, 1300
BfaI CTAG 2 cut(s) 159, 410
BfmI CTRYAG 3 cut(s) 231, 1162, 1271
BfuI GTATCC 1 cut(s) 1246
BglI GCCNNNNNGGC 1 cut(s) 381
BisI GCNGC 5 cut(s) 176, 261, 497, 505, 1271
BlsI GCNGC 5 cut(s) 177, 262, 498, 506, 1272
Bme1390I CCNGG 3 cut(s) 189, 436, 840
Bme18I GGWCC 3 cut(s) 368, 425, 836
BmgT120I GGNCC 6 cut(s) 190, 368, 425, 564, 565, 836
BmiI GGNNCC 4 cut(s) 317, 370, 458, 566
BmrFI CCNGG 3 cut(s) 189, 436, 840
BmsI GCATC 1 cut(s) 1159
BoxI GACNNNNGTC 1 cut(s) 430
Bpu10I CCTNAGC 1 cut(s) 150
Bpu14I TTCGAA 1 cut(s) 1138
BpuMI CCSGG 1 cut(s) 189
BsaBI GATNNNNATC 1 cut(s) 979
BsaJI CCNNGG 3 cut(s) 258, 311, 328
BsaXI ACNNNNNCTCC 2 cut(s) 1218, 1248
Bsc4I CCNNNNNNNGG 4 cut(s) 422, 466, 845, 1223
Bse118I RCCGGY 1 cut(s) 154
Bse1I ACTGG 3 cut(s) 370, 1292, 1335
Bse21I CCTNAGG 1 cut(s) 569
Bse8I GATNNNNATC 1 cut(s) 979
BseBI CCWGG 2 cut(s) 436, 840
BseDI CCNNGG 3 cut(s) 258, 311, 328
BseGI GGATG 1 cut(s) 1315
BseJI GATNNNNATC 1 cut(s) 979
BseLI CCNNNNNNNGG 4 cut(s) 422, 466, 845, 1223
BseMII CTCAG 4 cut(s) 141, 560, 771, 1212
BseNI ACTGG 3 cut(s) 370, 1292, 1335
BseSI GKGCMC 3 cut(s) 568, 1070, 1147
BseXI GCAGC 2 cut(s) 483, 1257
BsgI GTGCAG 1 cut(s) 129
Bsh1236I CGCG 1 cut(s) 260
Bsh1285I CGRYCG 1 cut(s) 1325
BshFI GGCC 4 cut(s) 192, 243, 439, 566
BshNI GGYRCC 1 cut(s) 456
BsiEI CGRYCG 1 cut(s) 1325
BsiHKAI GWGCWC 3 cut(s) 587, 1070, 1147
BsiSI CCGG 2 cut(s) 155, 188
BslFI GGGAC 6 cut(s) 150, 293, 354, 926, 943, 1138
BslI CCNNNNNNNGG 4 cut(s) 422, 466, 845, 1223
BsmAI GTCTC 4 cut(s) 869, 898, 947, 1300
BsmBI CGTCTC 1 cut(s) 898
BsmFI GGGAC 6 cut(s) 150, 293, 354, 926, 943, 1138
BsmI GAATGC 1 cut(s) 1261
BsnI GGCC 4 cut(s) 192, 243, 439, 566
Bsp119I TTCGAA 1 cut(s) 1138
Bsp120I GGGCCC 1 cut(s) 564
Bsp1286I GDGCHC 4 cut(s) 568, 587, 1070, 1147
Bsp143I GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
BspACI CCGC 9 cut(s) 176, 258, 260, 380, 504, 507, 687, 966, 1220
BspANI GGCC 4 cut(s) 192, 243, 439, 566
BspCNI CTCAG 4 cut(s) 142, 561, 770, 1211
BspFNI CGCG 1 cut(s) 260
BspHI TCATGA 2 cut(s) 342, 579
BspLI GGNNCC 4 cut(s) 317, 370, 458, 566
BspMAI CTGCAG 2 cut(s) 1166, 1275
BspPI GGATC 5 cut(s) 310, 323, 802, 956, 982
BspQI GCTCTTC 1 cut(s) 165
BspT104I TTCGAA 1 cut(s) 1138
BspT107I GGYRCC 1 cut(s) 456
BsrFI RCCGGY 1 cut(s) 154
BsrI ACTGG 3 cut(s) 370, 1292, 1335
BssAI RCCGGY 1 cut(s) 154
BssECI CCNNGG 3 cut(s) 258, 311, 328
BssMI GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
BssT1I CCWWGG 2 cut(s) 311, 328
Bst2UI CCWGG 2 cut(s) 436, 840
Bst4CI ACNGT 2 cut(s) 598, 907
Bst6I CTCTTC 1 cut(s) 165
BstBI TTCGAA 1 cut(s) 1138
BstC8I GCNNGC 7 cut(s) 156, 289, 382, 455, 885, 889, 1162
BstDEI CTNAG 5 cut(s) 150, 569, 608, 757, 1198
BstDSI CCRYGG 1 cut(s) 258
BstENI CCTNNNNNAGG 1 cut(s) 1221
BstF5I GGATG 1 cut(s) 1315
BstFNI CGCG 1 cut(s) 260
BstKTI GATC 6 cut(s) 221, 318, 797, 951, 977, 1325
BstMAI GTCTC 4 cut(s) 869, 898, 947, 1300
BstMBI GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
BstMCI CGRYCG 1 cut(s) 1325
BstMWI GCNNNNNNNGC 3 cut(s) 381, 463, 1276
BstNI CCWGG 2 cut(s) 436, 840
BstNSI RCATGY 1 cut(s) 891
BstPAI GACNNNNGTC 1 cut(s) 430
BstSCI CCNGG 3 cut(s) 187, 434, 838
BstSFI CTRYAG 3 cut(s) 231, 1162, 1271
BstSLI GKGCMC 3 cut(s) 568, 1070, 1147
BstUI CGCG 1 cut(s) 260
BstV1I GCAGC 2 cut(s) 483, 1257
BstX2I RGATCY 1 cut(s) 315
BstXI CCANNNNNNTGG 1 cut(s) 435
BstYI RGATCY 1 cut(s) 315
Bsu36I CCTNAGG 1 cut(s) 569
BsuI GTATCC 1 cut(s) 1246
BsuRI GGCC 4 cut(s) 192, 243, 439, 566
BtgI CCRYGG 1 cut(s) 258
BtsCI GGATG 1 cut(s) 1315
BtsI GCAGTG 3 cut(s) 20, 1092, 1171
BtsIMutI CAGTG 4 cut(s) 20, 363, 1092, 1171
Cac8I GCNNGC 7 cut(s) 156, 289, 382, 455, 885, 889, 1162
CciI TCATGA 2 cut(s) 342, 579
Cfr10I RCCGGY 1 cut(s) 154
Cfr13I GGNCC 6 cut(s) 190, 368, 425, 564, 565, 836
Cfr42I CCGCGG 1 cut(s) 261
CseI GACGC 2 cut(s) 880, 1285
CsiI ACCWGGT 1 cut(s) 838
CviAII CATG 8 cut(s) 276, 343, 580, 845, 888, 957, 1009, 1179
DdeI CTNAG 5 cut(s) 150, 569, 608, 757, 1198
DpnI GATC 6 cut(s) 220, 317, 796, 950, 976, 1324
DpnII GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
EaeI YGGCCR 2 cut(s) 241, 437
Eam1104I CTCTTC 1 cut(s) 165
EarI CTCTTC 1 cut(s) 165
EciI GGCGGA 1 cut(s) 676
Ecl136II GAGCTC 1 cut(s) 585
Eco130I CCWWGG 2 cut(s) 311, 328
Eco24I GRGCYC 2 cut(s) 568, 587
Eco47I GGWCC 3 cut(s) 368, 425, 836
Eco53kI GAGCTC 1 cut(s) 585
Eco57I CTGAAG 1 cut(s) 1065
Eco81I CCTNAGG 1 cut(s) 569
EcoICRI GAGCTC 1 cut(s) 585
EcoNI CCTNNNNNAGG 1 cut(s) 1221
EcoO109I RGGNCCY 2 cut(s) 564, 565
EcoRII CCWGG 2 cut(s) 434, 838
EcoT14I CCWWGG 2 cut(s) 311, 328
EcoT22I ATGCAT 1 cut(s) 1155
EcoT38I GRGCYC 2 cut(s) 568, 587
ErhI CCWWGG 2 cut(s) 311, 328
Esp3I CGTCTC 1 cut(s) 898
FaeI CATG 8 cut(s) 279, 346, 583, 848, 891, 960, 1012, 1182
FalI AAGNNNNNCTT 2 cut(s) 1194, 1226
FaqI GGGAC 6 cut(s) 150, 293, 354, 926, 943, 1138
FatI CATG 8 cut(s) 275, 342, 579, 844, 887, 956, 1008, 1178
FauI CCCGC 2 cut(s) 373, 959
FblI GTMKAC 1 cut(s) 813
Fnu4HI GCNGC 5 cut(s) 176, 261, 497, 505, 1271
FokI GGATG 1 cut(s) 1322
FriOI GRGCYC 2 cut(s) 568, 587
Fsp4HI GCNGC 5 cut(s) 176, 261, 497, 505, 1271
FspBI CTAG 2 cut(s) 159, 410
GluI GCNGC 5 cut(s) 176, 261, 497, 505, 1271
HaeIII GGCC 4 cut(s) 192, 243, 439, 566
HapII CCGG 2 cut(s) 155, 188
HgaI GACGC 2 cut(s) 880, 1285
Hin1II CATG 8 cut(s) 279, 346, 583, 848, 891, 960, 1012, 1182
HincII GTYRAC 1 cut(s) 484
HindII GTYRAC 1 cut(s) 484
HinfI GANTC 9 cut(s) 302, 431, 480, 541, 671, 718, 849, 862, 914
HpaII CCGG 2 cut(s) 155, 188
HphI GGTGA 1 cut(s) 785
Hpy166II GTNNAC 6 cut(s) 66, 484, 814, 836, 1068, 1145
Hpy188I TCNGA 6 cut(s) 202, 705, 869, 1099, 1201, 1240
Hpy188III TCNNGA 5 cut(s) 167, 343, 580, 952, 972
Hpy8I GTNNAC 6 cut(s) 66, 484, 814, 836, 1068, 1145
HpyAV CCTTC 1 cut(s) 147
HpyCH4III ACNGT 2 cut(s) 598, 907
HpyCH4IV ACGT 2 cut(s) 1134, 1341
HpyF10VI GCNNNNNNNGC 3 cut(s) 381, 463, 1276
HpyF3I CTNAG 5 cut(s) 150, 569, 608, 757, 1198
HpySE526I ACGT 2 cut(s) 1134, 1341
Hsp92II CATG 8 cut(s) 279, 346, 583, 848, 891, 960, 1012, 1182
KroI GCCGGC 1 cut(s) 154
KroNI GCCGGC 1 cut(s) 156
KspI CCGCGG 1 cut(s) 261
Kzo9I GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
LguI GCTCTTC 1 cut(s) 165
Lsp1109I GCAGC 2 cut(s) 483, 1257
LweI GCATC 1 cut(s) 1159
MabI ACCWGGT 1 cut(s) 838
MaeI CTAG 2 cut(s) 159, 410
MaeII ACGT 2 cut(s) 1134, 1341
MaeIII GTNAC 3 cut(s) 18, 390, 988
MalI GATC 6 cut(s) 220, 317, 796, 950, 976, 1324
MboI GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
MboII GAAGA 5 cut(s) 16, 182, 536, 568, 1205
MfeI CAATTG 1 cut(s) 789
MflI RGATCY 1 cut(s) 315
MhlI GDGCHC 4 cut(s) 568, 587, 1070, 1147
MlsI TGGCCA 2 cut(s) 243, 439
MluCI AATT 9 cut(s) 7, 513, 612, 629, 649, 747, 789, 877, 1284
MluNI TGGCCA 2 cut(s) 243, 439
MlyI GAGTC 4 cut(s) 440, 489, 871, 908
MmeI TCCRAC 3 cut(s) 1232, 1263, 1287
MnlI CCTC 6 cut(s) 144, 151, 208, 518, 564, 1329
Mox20I TGGCCA 2 cut(s) 243, 439
Mph1103I ATGCAT 1 cut(s) 1155
MroNI GCCGGC 1 cut(s) 154
MscI TGGCCA 2 cut(s) 243, 439
MseI TTAA 2 cut(s) 267, 615
Msp20I TGGCCA 2 cut(s) 243, 439
MspA1I CMGCKG 1 cut(s) 260
MspI CCGG 2 cut(s) 155, 188
MspR9I CCNGG 3 cut(s) 189, 436, 840
MunI CAATTG 1 cut(s) 789
Mva1269I GAATGC 1 cut(s) 1261
MvaI CCWGG 2 cut(s) 436, 840
MvnI CGCG 1 cut(s) 260
MwoI GCNNNNNNNGC 3 cut(s) 381, 463, 1276
NaeI GCCGGC 1 cut(s) 156
NciI CCSGG 1 cut(s) 189
NdeII GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
NgoMIV GCCGGC 1 cut(s) 154
NlaIII CATG 8 cut(s) 279, 346, 583, 848, 891, 960, 1012, 1182
NlaIV GGNNCC 4 cut(s) 317, 370, 458, 566
NmuCI GTSAC 1 cut(s) 18
NsiI ATGCAT 1 cut(s) 1155
NspI RCATGY 1 cut(s) 891
NspV TTCGAA 1 cut(s) 1138
PaeI GCATGC 1 cut(s) 891
PagI TCATGA 2 cut(s) 342, 579
PciSI GCTCTTC 1 cut(s) 165
PctI GAATGC 1 cut(s) 1261
PdiI GCCGGC 1 cut(s) 156
PfeI GAWTC 5 cut(s) 302, 541, 671, 718, 849
PflFI GACNNNGTC 1 cut(s) 840
PflMI CCANNNNNTGG 2 cut(s) 422, 466
PkrI GCNGC 5 cut(s) 177, 262, 498, 506, 1272
Ple19I CGATCG 1 cut(s) 1325
PleI GAGTC 4 cut(s) 439, 488, 870, 908
PpsI GAGTC 4 cut(s) 439, 488, 870, 908
PshAI GACNNNNGTC 1 cut(s) 430
PsiI TTATAA 1 cut(s) 753
Psp124BI GAGCTC 1 cut(s) 587
Psp6I CCWGG 2 cut(s) 434, 838
PspGI CCWGG 2 cut(s) 434, 838
PspN4I GGNNCC 4 cut(s) 317, 370, 458, 566
PspOMI GGGCCC 1 cut(s) 564
PspPI GGNCC 6 cut(s) 190, 368, 425, 564, 565, 836
PstI CTGCAG 2 cut(s) 1166, 1275
PsuI RGATCY 1 cut(s) 315
PsyI GACNNNGTC 1 cut(s) 840
PvuI CGATCG 1 cut(s) 1325
SacI GAGCTC 1 cut(s) 587
SacII CCGCGG 1 cut(s) 261
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 2 cut(s) 267, 615
SatI GCNGC 5 cut(s) 176, 261, 497, 505, 1271
Sau3AI GATC 6 cut(s) 218, 315, 794, 948, 974, 1322
Sau96I GGNCC 6 cut(s) 190, 368, 425, 564, 565, 836
SchI GAGTC 4 cut(s) 440, 489, 871, 908
ScrFI CCNGG 3 cut(s) 189, 436, 840
SduI GDGCHC 4 cut(s) 568, 587, 1070, 1147
SexAI ACCWGGT 1 cut(s) 838
SfaNI GCATC 1 cut(s) 1159
SfcI CTRYAG 3 cut(s) 231, 1162, 1271
Sfr303I CCGCGG 1 cut(s) 261
SfuI TTCGAA 1 cut(s) 1138
SgrBI CCGCGG 1 cut(s) 261
SinI GGWCC 3 cut(s) 368, 425, 836
SphI GCATGC 1 cut(s) 891
Sse9I AATT 9 cut(s) 7, 513, 612, 629, 649, 747, 789, 877, 1284
SsiI CCGC 9 cut(s) 176, 258, 260, 380, 504, 507, 687, 966, 1220
SspI AATATT 2 cut(s) 360, 1003
SspMI CTAG 2 cut(s) 159, 410
SstI GAGCTC 1 cut(s) 587
StyD4I CCNGG 3 cut(s) 187, 434, 838
StyI CCWWGG 2 cut(s) 311, 328
TaaI ACNGT 2 cut(s) 598, 907
TaiI ACGT 2 cut(s) 1137, 1344
TaqI TCGA 5 cut(s) 210, 917, 951, 1138, 1321
TasI AATT 9 cut(s) 7, 513, 612, 629, 649, 747, 789, 877, 1284
TauI GCSGC 3 cut(s) 178, 263, 507
TfiI GAWTC 5 cut(s) 302, 541, 671, 718, 849
Tru1I TTAA 2 cut(s) 267, 615
Tru9I TTAA 2 cut(s) 267, 615
TscAI CASTG 4 cut(s) 27, 370, 1092, 1171
TseFI GTSAC 1 cut(s) 18
TseI GCWGC 2 cut(s) 496, 1270
Tsp45I GTSAC 1 cut(s) 18
TspDTI ATGAA 4 cut(s) 17, 315, 331, 568
TspGWI ACGGA 2 cut(s) 835, 947
TspRI CASTG 4 cut(s) 27, 370, 1092, 1171
Tth111I GACNNNGTC 1 cut(s) 840
Van91I CCANNNNNTGG 2 cut(s) 422, 466
VneI GTGCAC 2 cut(s) 1066, 1143
VpaK11BI GGWCC 3 cut(s) 368, 425, 836
XagI CCTNNNNNAGG 1 cut(s) 1221
XapI RAATTY 1 cut(s) 747
XceI RCATGY 1 cut(s) 891
XmiI GTMKAC 1 cut(s) 813
XspI CTAG 2 cut(s) 159, 410
Zsp2I ATGCAT 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.