pycom461g00010

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0017912
Physical Location & Seq
Reverse (-)
7517 .. 8435
919 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom461g00010.2

Sequence Viewer

Length: 762 bp
ATGCTACTGAGAAGATCCTTCGACTTCCTCTTTAGTCGACTCGGGTGGGAAGTAGGTCTATTTATGGCTATTATCTTGGCTCTGTTTGATGCTGAATCACCCGCTATAGGGAACATGATGGCACCATCCGGAGCTTCCGGCGCATCCAACTCTGGGAATTGGCGTCAATATCTAAACTTCTCCTCAGAGAACGAAGGAGATTCCGCCCCCGAGCCATCCACAGCCCGGGCCCCCGTAAGGGAAGCAATCGGGAGAGATTTGGACCAGGCGGGCCCCTCCTCTTTTACGGAGGATTCGTTTGACGTGCGGGTTCTCTTGGAAGCATTTACCGGTCGCTTCCCGGGGGAGGAAGCAGGTCCGTCTAATTGGGTCCCCCCAGAAGAAAAATTCCCCTATCACCCGGATGAAGTGATAGGAGGAGATTCTGTTAGCTCGATTGAGTGGCGCCTTTTGGCGAAAGTGAATTCTCCCACAGCCGAGGACATCAAATTCGCCAAAATTAGGGCCGAAGATCTTTTCGAGATCAAGGTTCAAATTGTACGGCATATGGCAGGCCTTGATCCGGAAGGCGATTGGCTGGGGCGCGGGGCTCGGGCCCTCGACAATCCGCGTACCGCCACGGGAGAACCCTCCTTAGAACGACTCTATGACCTTCTGGAGGACCTAAACCGGGGCGGAGTCCAATCAGAGACCTTCTCTTACTTAAAGAATAAGGTCTTAAGCAAAAGAGAGGAGGTTGGCGACGACAGCAGCATGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

254

Amino Acids

27.86

Weight (kDa)

4.6

Isoelectric Point (pI)

55.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07629 AT2G07629 AT2G07674 AT2G07674 ATMG00670 ATMG00690 ATMG01010 ATMG01020 ATMG01200
pyrus_communis pycom461g00010 pycom461g00330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 344
AccB1I GGYRCC 2 cut(s) 121, 444
AccI GTMKAC 1 cut(s) 37
AccII CGCG 2 cut(s) 585, 610
AccIII TCCGGA 2 cut(s) 128, 562
AciI CCGC 8 cut(s) 102, 204, 269, 307, 585, 608, 615, 675
AclWI GGATC 2 cut(s) 9, 554
AcsI RAATTY 3 cut(s) 386, 463, 488
AcyI GRCGYC 2 cut(s) 163, 445
AfaI GTAC 2 cut(s) 540, 613
AflII CTTAAG 1 cut(s) 718
AgeI ACCGGT 1 cut(s) 329
AgsI TTSAA 1 cut(s) 533
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 264
AluBI AGCT 2 cut(s) 134, 432
AluI AGCT 2 cut(s) 134, 432
Alw26I GTCTC 1 cut(s) 683
AlwI GGATC 2 cut(s) 9, 554
Ama87I CYCGRG 5 cut(s) 41, 209, 225, 340, 591
Aor13HI TCCGGA 2 cut(s) 128, 562
AoxI GGCC 5 cut(s) 228, 271, 504, 553, 594
ApaI GGGCCC 3 cut(s) 232, 275, 598
ApeKI GCWGC 1 cut(s) 750
ApoI RAATTY 3 cut(s) 386, 463, 488
ArsI GACNNNNNNTTYG 2 cut(s) 473, 505
AsiGI ACCGGT 1 cut(s) 329
AspLEI GCGC 3 cut(s) 143, 447, 585
AsuC2I CCSGG 6 cut(s) 226, 227, 341, 342, 401, 671
AsuHPI GGTGA 2 cut(s) 90, 389
AvaI CYCGRG 5 cut(s) 41, 209, 225, 340, 591
AvaII GGWCC 4 cut(s) 262, 356, 370, 661
BaeGI GKGCMC 3 cut(s) 232, 275, 598
BanI GGYRCC 2 cut(s) 121, 444
BanII GRGCYC 4 cut(s) 232, 275, 592, 598
BccI CCATC 3 cut(s) 112, 133, 223
BceAI ACGGC 1 cut(s) 557
BciT130I CCWGG 1 cut(s) 266
BcnI CCSGG 6 cut(s) 226, 227, 341, 342, 401, 671
BcoDI GTCTC 1 cut(s) 683
BfmI CTRYAG 1 cut(s) 105
BfoI RGCGCY 1 cut(s) 448
BfrI CTTAAG 1 cut(s) 718
BfuAI ACCTGC 1 cut(s) 344
BglII AGATCT 1 cut(s) 511
BisI GCNGC 1 cut(s) 751
BlsI GCNGC 1 cut(s) 752
Bme1390I CCNGG 7 cut(s) 226, 227, 266, 341, 342, 401, 671
Bme18I GGWCC 4 cut(s) 262, 356, 370, 661
BmeT110I CYCGRG 5 cut(s) 41, 209, 225, 340, 591
BmgBI CACGTC 1 cut(s) 304
BmiI GGNNCC 9 cut(s) 123, 230, 231, 273, 274, 371, 372, 446, 596
BmrFI CCNGG 7 cut(s) 226, 227, 266, 341, 342, 401, 671
BmsI GCATC 2 cut(s) 79, 152
BplI GAGNNNNNCTC 2 cut(s) 680, 712
BpmI CTGGAG 1 cut(s) 677
BpuMI CCSGG 6 cut(s) 226, 227, 341, 342, 401, 671
BsaHI GRCGYC 2 cut(s) 163, 445
BsaI GGTCTC 1 cut(s) 683
BsaJI CCNNGG 6 cut(s) 225, 340, 341, 477, 618, 670
BsaWI WCCGGW 3 cut(s) 128, 329, 562
BsaXI ACNNNNNCTCC 4 cut(s) 281, 311, 411, 441
Bse118I RCCGGY 1 cut(s) 329
BseAI TCCGGA 2 cut(s) 128, 562
BseBI CCWGG 1 cut(s) 266
BseDI CCNNGG 6 cut(s) 225, 340, 341, 477, 618, 670
BseGI GGATG 4 cut(s) 125, 143, 215, 409
BseMII CTCAG 1 cut(s) 198
BseRI GAGGAG 4 cut(s) 172, 268, 432, 746
BseSI GKGCMC 3 cut(s) 232, 275, 598
BseYI CCCAGC 1 cut(s) 577
Bsh1236I CGCG 2 cut(s) 585, 610
Bsh1285I CGRYCG 1 cut(s) 334
BshFI GGCC 5 cut(s) 230, 273, 506, 555, 596
BshNI GGYRCC 2 cut(s) 121, 444
BshTI ACCGGT 1 cut(s) 329
BsiEI CGRYCG 1 cut(s) 334
BsiHKCI CYCGRG 5 cut(s) 41, 209, 225, 340, 591
BsiSI CCGG 8 cut(s) 129, 138, 226, 330, 341, 401, 563, 670
BslFI GGGAC 1 cut(s) 356
BsmAI GTCTC 1 cut(s) 683
BsmFI GGGAC 1 cut(s) 356
BsnI GGCC 5 cut(s) 230, 273, 506, 555, 596
Bso31I GGTCTC 1 cut(s) 683
BsoBI CYCGRG 5 cut(s) 41, 209, 225, 340, 591
Bsp120I GGGCCC 3 cut(s) 228, 271, 594
Bsp1286I GDGCHC 4 cut(s) 232, 275, 592, 598
Bsp13I TCCGGA 2 cut(s) 128, 562
Bsp143I GATC 4 cut(s) 14, 511, 522, 559
BspACI CCGC 8 cut(s) 102, 204, 269, 307, 585, 608, 615, 675
BspANI GGCC 5 cut(s) 230, 273, 506, 555, 596
BspCNI CTCAG 1 cut(s) 197
BspEI TCCGGA 2 cut(s) 128, 562
BspFNI CGCG 2 cut(s) 585, 610
BspLI GGNNCC 9 cut(s) 123, 230, 231, 273, 274, 371, 372, 446, 596
BspMI ACCTGC 1 cut(s) 344
BspPI GGATC 2 cut(s) 9, 554
BspT107I GGYRCC 2 cut(s) 121, 444
BspTI CTTAAG 1 cut(s) 718
BspTNI GGTCTC 1 cut(s) 683
BsrFI RCCGGY 1 cut(s) 329
BssAI RCCGGY 1 cut(s) 329
BssECI CCNNGG 6 cut(s) 225, 340, 341, 477, 618, 670
BssMI GATC 4 cut(s) 14, 511, 522, 559
BssNI GRCGYC 2 cut(s) 163, 445
Bst2UI CCWGG 1 cut(s) 266
BstACI GRCGYC 2 cut(s) 163, 445
BstAFI CTTAAG 1 cut(s) 718
BstC8I GCNNGC 2 cut(s) 271, 553
BstDEI CTNAG 3 cut(s) 8, 184, 634
BstDSI CCRYGG 1 cut(s) 618
BstENI CCTNNNNNAGG 1 cut(s) 656
BstF5I GGATG 4 cut(s) 125, 143, 215, 409
BstFNI CGCG 2 cut(s) 585, 610
BstH2I RGCGCY 1 cut(s) 448
BstHHI GCGC 3 cut(s) 143, 447, 585
BstKTI GATC 4 cut(s) 17, 514, 525, 562
BstMAI GTCTC 1 cut(s) 683
BstMBI GATC 4 cut(s) 14, 511, 522, 559
BstMCI CGRYCG 1 cut(s) 334
BstMWI GCNNNNNNNGC 2 cut(s) 140, 747
BstNI CCWGG 1 cut(s) 266
BstNSI RCATGY 1 cut(s) 757
BstSCI CCNGG 7 cut(s) 224, 225, 264, 339, 340, 399, 669
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 3 cut(s) 232, 275, 598
BstUI CGCG 2 cut(s) 585, 610
BstX2I RGATCY 2 cut(s) 14, 511
BstYI RGATCY 2 cut(s) 14, 511
BsuRI GGCC 5 cut(s) 230, 273, 506, 555, 596
BtgI CCRYGG 1 cut(s) 618
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 4 cut(s) 125, 143, 215, 409
BveI ACCTGC 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 271, 553
CfoI GCGC 3 cut(s) 143, 447, 585
Cfr10I RCCGGY 1 cut(s) 329
Cfr9I CCCGGG 2 cut(s) 225, 340
CseI GACGC 1 cut(s) 152
Csp6I GTAC 2 cut(s) 539, 612
CspAI ACCGGT 1 cut(s) 329
CviAII CATG 2 cut(s) 115, 754
CviQI GTAC 2 cut(s) 539, 612
DdeI CTNAG 3 cut(s) 8, 184, 634
DinI GGCGCC 1 cut(s) 446
DpnI GATC 4 cut(s) 16, 513, 524, 561
DpnII GATC 4 cut(s) 14, 511, 522, 559
EciI GGCGGA 2 cut(s) 193, 690
Eco147I AGGCCT 1 cut(s) 555
Eco24I GRGCYC 4 cut(s) 232, 275, 592, 598
Eco31I GGTCTC 1 cut(s) 683
Eco47I GGWCC 4 cut(s) 262, 356, 370, 661
Eco88I CYCGRG 5 cut(s) 41, 209, 225, 340, 591
EcoNI CCTNNNNNAGG 1 cut(s) 656
EcoO109I RGGNCCY 5 cut(s) 229, 272, 370, 595, 661
EcoRI GAATTC 1 cut(s) 463
EcoRII CCWGG 1 cut(s) 264
EcoT38I GRGCYC 4 cut(s) 232, 275, 592, 598
EgeI GGCGCC 1 cut(s) 446
EheI GGCGCC 1 cut(s) 446
FaeI CATG 2 cut(s) 118, 757
FaiI YATR 7 cut(s) 65, 107, 116, 546, 548, 648, 755
FaqI GGGAC 1 cut(s) 356
FatI CATG 2 cut(s) 114, 753
FauI CCCGC 4 cut(s) 109, 262, 300, 578
FauNDI CATATG 1 cut(s) 546
FblI GTMKAC 1 cut(s) 37
Fnu4HI GCNGC 1 cut(s) 751
FokI GGATG 4 cut(s) 112, 130, 202, 416
FriOI GRGCYC 4 cut(s) 232, 275, 592, 598
Fsp4HI GCNGC 1 cut(s) 751
GlaI GCGC 3 cut(s) 142, 446, 584
GluI GCNGC 1 cut(s) 751
GsaI CCCAGC 1 cut(s) 581
GsuI CTGGAG 1 cut(s) 677
HaeII RGCGCY 1 cut(s) 448
HaeIII GGCC 5 cut(s) 230, 273, 506, 555, 596
HapII CCGG 8 cut(s) 129, 138, 226, 330, 341, 401, 563, 670
HgaI GACGC 1 cut(s) 152
HhaI GCGC 3 cut(s) 143, 447, 585
Hin1I GRCGYC 2 cut(s) 163, 445
Hin1II CATG 2 cut(s) 118, 757
Hin6I GCGC 3 cut(s) 141, 445, 583
HinP1I GCGC 3 cut(s) 141, 445, 583
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HinfI GANTC 7 cut(s) 39, 95, 200, 293, 422, 642, 678
HpaII CCGG 8 cut(s) 129, 138, 226, 330, 341, 401, 563, 670
HphI GGTGA 2 cut(s) 90, 389
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 2 cut(s) 187, 688
Hpy188III TCNNGA 5 cut(s) 129, 250, 520, 563, 656
Hpy8I GTNNAC 1 cut(s) 38
Hpy99I CGWCG 1 cut(s) 746
HpyAV CCTTC 5 cut(s) 28, 188, 560, 662, 703
HpyCH4IV ACGT 1 cut(s) 303
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 747
HpyF3I CTNAG 3 cut(s) 8, 184, 634
HpySE526I ACGT 1 cut(s) 303
Hsp92I GRCGYC 2 cut(s) 163, 445
Hsp92II CATG 2 cut(s) 118, 757
HspAI GCGC 3 cut(s) 141, 445, 583
KasI GGCGCC 1 cut(s) 444
KflI GGGWCCC 1 cut(s) 370
Kpn2I TCCGGA 2 cut(s) 128, 562
Kzo9I GATC 4 cut(s) 14, 511, 522, 559
LmnI GCTCC 1 cut(s) 131
LweI GCATC 2 cut(s) 79, 152
MaeII ACGT 1 cut(s) 303
MalI GATC 4 cut(s) 16, 513, 524, 561
MboI GATC 4 cut(s) 14, 511, 522, 559
MboII GAAGA 3 cut(s) 24, 392, 521
MflI RGATCY 2 cut(s) 14, 511
MhlI GDGCHC 4 cut(s) 232, 275, 592, 598
MluCI AATT 7 cut(s) 157, 364, 386, 463, 488, 498, 534
Mly113I GGCGCC 1 cut(s) 445
MlyI GAGTC 3 cut(s) 33, 636, 687
MmeI TCCRAC 1 cut(s) 171
MroI TCCGGA 2 cut(s) 128, 562
MseI TTAA 2 cut(s) 704, 719
MslI CAYNNNNRTG 1 cut(s) 402
MspCI CTTAAG 1 cut(s) 718
MspI CCGG 8 cut(s) 129, 138, 226, 330, 341, 401, 563, 670
MspR9I CCNGG 7 cut(s) 226, 227, 266, 341, 342, 401, 671
MvaI CCWGG 1 cut(s) 266
MvnI CGCG 2 cut(s) 585, 610
MwoI GCNNNNNNNGC 2 cut(s) 140, 747
NarI GGCGCC 1 cut(s) 445
NciI CCSGG 6 cut(s) 226, 227, 341, 342, 401, 671
NdeI CATATG 1 cut(s) 546
NdeII GATC 4 cut(s) 14, 511, 522, 559
NlaIII CATG 2 cut(s) 118, 757
NlaIV GGNNCC 9 cut(s) 123, 230, 231, 273, 274, 371, 372, 446, 596
NmeAIII GCCGAG 1 cut(s) 502
NspI RCATGY 1 cut(s) 757
PceI AGGCCT 1 cut(s) 555
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PfeI GAWTC 4 cut(s) 95, 200, 293, 422
PinAI ACCGGT 1 cut(s) 329
PkrI GCNGC 1 cut(s) 752
PleI GAGTC 3 cut(s) 33, 636, 686
PluTI GGCGCC 1 cut(s) 448
PpsI GAGTC 3 cut(s) 33, 636, 686
PpuMI RGGWCCY 2 cut(s) 370, 661
Psp5II RGGWCCY 2 cut(s) 370, 661
Psp6I CCWGG 1 cut(s) 264
PspFI CCCAGC 1 cut(s) 577
PspGI CCWGG 1 cut(s) 264
PspN4I GGNNCC 9 cut(s) 123, 230, 231, 273, 274, 371, 372, 446, 596
PspOMI GGGCCC 3 cut(s) 228, 271, 594
PspPPI RGGWCCY 2 cut(s) 370, 661
PsuI RGATCY 2 cut(s) 14, 511
RsaI GTAC 2 cut(s) 540, 613
RsaNI GTAC 2 cut(s) 539, 612
RseI CAYNNNNRTG 1 cut(s) 402
SalI GTCGAC 1 cut(s) 36
SaqAI TTAA 2 cut(s) 704, 719
SatI GCNGC 1 cut(s) 751
Sau3AI GATC 4 cut(s) 14, 511, 522, 559
SchI GAGTC 3 cut(s) 33, 636, 687
ScrFI CCNGG 7 cut(s) 226, 227, 266, 341, 342, 401, 671
SduI GDGCHC 4 cut(s) 232, 275, 592, 598
SfaNI GCATC 2 cut(s) 79, 152
SfcI CTRYAG 1 cut(s) 105
SfoI GGCGCC 1 cut(s) 446
SinI GGWCC 4 cut(s) 262, 356, 370, 661
SmaI CCCGGG 2 cut(s) 227, 342
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 718
SmoI CTYRAG 1 cut(s) 718
SrfI GCCCGGGC 1 cut(s) 227
Sse9I AATT 7 cut(s) 157, 364, 386, 463, 488, 498, 534
SseBI AGGCCT 1 cut(s) 555
SsiI CCGC 8 cut(s) 102, 204, 269, 307, 585, 608, 615, 675
SspDI GGCGCC 1 cut(s) 444
StuI AGGCCT 1 cut(s) 555
StyD4I CCNGG 7 cut(s) 224, 225, 264, 339, 340, 399, 669
TaiI ACGT 1 cut(s) 306
TaqI TCGA 5 cut(s) 21, 37, 434, 519, 600
TasI AATT 7 cut(s) 157, 364, 386, 463, 488, 498, 534
TfiI GAWTC 4 cut(s) 95, 200, 293, 422
Tru1I TTAA 2 cut(s) 704, 719
Tru9I TTAA 2 cut(s) 704, 719
TseI GCWGC 1 cut(s) 750
TspDTI ATGAA 1 cut(s) 420
TspGWI ACGGA 2 cut(s) 302, 348
TspMI CCCGGG 2 cut(s) 225, 340
Vha464I CTTAAG 1 cut(s) 718
VpaK11BI GGWCC 4 cut(s) 262, 356, 370, 661
XagI CCTNNNNNAGG 1 cut(s) 656
XapI RAATTY 3 cut(s) 386, 463, 488
XceI RCATGY 1 cut(s) 757
XmaI CCCGGG 2 cut(s) 225, 340
XmiI GTMKAC 1 cut(s) 37
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.