pycom461g00330

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0017912
Physical Location & Seq
Reverse (-)
267865 .. 268785
921 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 921 bp
ATGCTACTGAGAAGATCCTTCGACTTCCTCTTTAGTCGACTCGGGTGGGAAGTAGGTCTATTTATGGCTATTATCTTGGCTCTGTTTGATGCTGAATCACCCGCTATAGGGAACATGATGGCACCATCCGGAGCTTCCGGCGCATCCAACTCTGGGAATTGGCGTCAATATCTAAACTTCTCCTCAGAGAACGAAGGAGATTCCGCCCCCGAGCCATCCACAGCCCGGGCCCCCGTAAGGGAAGCAATCGGGAGAGATTTGGACCAGGCGGGCCCCTCCTCTTTTACGGAGGATTCGTTTGACGTGCGGGTTCTCTTGGAAGCATTTAGCAAATCCGAAACAACGGGGGAAACCTCGGTAAATCGGGGGGGTAGCAGTAGGGGTTCTGGTTGGACTTCTTTCGATATAGACGTCTTTTTGGATTCCTTTACTAATGAGGAAGGCGAAGAAGTAGCCCAGCCGCACCCCCAAAACGCCCCTGCTAATCCGGTCGCTTCCCGGGGGGAGGAAGCAGGTCCGTCTAATTGGGTCCCCCCAGAAGAAAAATTCCCCTATCACCCGGATGAAGTGATAGGAGGAGATTCTGTTAGCTCGATTGAGTGGCGCCTTTTGGCGAAAGTGAATTCTCCCACAGCCGAGGACATCAAATTCGCCAAAATTAGGGCCGAAGATCTTTTCGAGATCAAGGTTCAAATTGTACGGCATATGGCAGGCCTTGATCCGGAAGGCGATTGGCTGGGGCGCGGGGCTCGGGCCCTCGACAATCCGCGTACCGCCACGGGAGAACCCTCCTTAGAACGACTCTATGACCTTCTGGAGGACCTAAACCGGGGCGGAGTCCAATCAGAGACCTTCTCTTACTTAAAGAATAAGGTCTTAAGCAAAAGAGAGGAGGTTGGCGACGACAGCAGCATGTCGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

33.43

Weight (kDa)

4.52

Isoelectric Point (pI)

54.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF8018 PF26057 188 - 289 2.9e-47 Domain of unknown function (DUF8018)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017912)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G07629 AT2G07629 AT2G07674 AT2G07674 ATMG00670 ATMG00690 ATMG01010 ATMG01020 ATMG01200
pyrus_communis pycom461g00010 pycom461g00330

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 414
Acc36I ACCTGC 1 cut(s) 503
AccB1I GGYRCC 2 cut(s) 121, 603
AccI GTMKAC 1 cut(s) 37
AccII CGCG 2 cut(s) 744, 769
AccIII TCCGGA 2 cut(s) 128, 721
AciI CCGC 9 cut(s) 102, 204, 269, 307, 461, 744, 767, 774, 834
AclWI GGATC 2 cut(s) 9, 713
AcsI RAATTY 3 cut(s) 545, 622, 647
AcyI GRCGYC 3 cut(s) 163, 411, 604
AfaI GTAC 2 cut(s) 699, 772
AflII CTTAAG 1 cut(s) 877
AgsI TTSAA 1 cut(s) 692
AjiI CACGTC 1 cut(s) 304
AjnI CCWGG 1 cut(s) 264
AluBI AGCT 2 cut(s) 134, 591
AluI AGCT 2 cut(s) 134, 591
Alw26I GTCTC 1 cut(s) 842
AlwI GGATC 2 cut(s) 9, 713
Ama87I CYCGRG 5 cut(s) 41, 209, 225, 498, 750
Aor13HI TCCGGA 2 cut(s) 128, 721
AoxI GGCC 5 cut(s) 228, 271, 663, 712, 753
ApaI GGGCCC 3 cut(s) 232, 275, 757
ApeKI GCWGC 1 cut(s) 909
ApoI RAATTY 3 cut(s) 545, 622, 647
ArsI GACNNNNNNTTYG 2 cut(s) 632, 664
AspLEI GCGC 3 cut(s) 143, 606, 744
AsuC2I CCSGG 6 cut(s) 226, 227, 499, 500, 560, 830
AsuHPI GGTGA 2 cut(s) 90, 548
AvaI CYCGRG 5 cut(s) 41, 209, 225, 498, 750
AvaII GGWCC 4 cut(s) 262, 515, 529, 820
BaeGI GKGCMC 3 cut(s) 232, 275, 757
BanI GGYRCC 2 cut(s) 121, 603
BanII GRGCYC 4 cut(s) 232, 275, 751, 757
BccI CCATC 3 cut(s) 112, 133, 223
BceAI ACGGC 1 cut(s) 716
BciT130I CCWGG 1 cut(s) 266
BcnI CCSGG 6 cut(s) 226, 227, 499, 500, 560, 830
BcoDI GTCTC 1 cut(s) 842
BfmI CTRYAG 1 cut(s) 105
BfoI RGCGCY 1 cut(s) 607
BfrI CTTAAG 1 cut(s) 877
BfuAI ACCTGC 1 cut(s) 503
BglII AGATCT 1 cut(s) 670
BisI GCNGC 2 cut(s) 461, 910
BlsI GCNGC 2 cut(s) 462, 911
Bme1390I CCNGG 7 cut(s) 226, 227, 266, 499, 500, 560, 830
Bme18I GGWCC 4 cut(s) 262, 515, 529, 820
BmeT110I CYCGRG 5 cut(s) 41, 209, 225, 498, 750
BmgBI CACGTC 1 cut(s) 304
BmiI GGNNCC 9 cut(s) 123, 230, 231, 273, 274, 530, 531, 605, 755
BmrFI CCNGG 7 cut(s) 226, 227, 266, 499, 500, 560, 830
BmsI GCATC 2 cut(s) 79, 152
BplI GAGNNNNNCTC 2 cut(s) 839, 871
BpmI CTGGAG 1 cut(s) 836
BpuMI CCSGG 6 cut(s) 226, 227, 499, 500, 560, 830
BsaHI GRCGYC 3 cut(s) 163, 411, 604
BsaI GGTCTC 1 cut(s) 842
BsaJI CCNNGG 7 cut(s) 225, 354, 498, 499, 636, 777, 829
BsaWI WCCGGW 3 cut(s) 128, 487, 721
BsaXI ACNNNNNCTCC 4 cut(s) 281, 311, 570, 600
BseAI TCCGGA 2 cut(s) 128, 721
BseBI CCWGG 1 cut(s) 266
BseDI CCNNGG 7 cut(s) 225, 354, 498, 499, 636, 777, 829
BseGI GGATG 4 cut(s) 125, 143, 215, 568
BseMII CTCAG 1 cut(s) 198
BseRI GAGGAG 4 cut(s) 172, 268, 591, 905
BseSI GKGCMC 3 cut(s) 232, 275, 757
BseYI CCCAGC 2 cut(s) 456, 736
Bsh1236I CGCG 2 cut(s) 744, 769
Bsh1285I CGRYCG 1 cut(s) 492
BshFI GGCC 5 cut(s) 230, 273, 665, 714, 755
BshNI GGYRCC 2 cut(s) 121, 603
BsiEI CGRYCG 1 cut(s) 492
BsiHKCI CYCGRG 5 cut(s) 41, 209, 225, 498, 750
BsiSI CCGG 8 cut(s) 129, 138, 226, 488, 499, 560, 722, 829
BslFI GGGAC 1 cut(s) 515
BsmAI GTCTC 1 cut(s) 842
BsmFI GGGAC 1 cut(s) 515
BsnI GGCC 5 cut(s) 230, 273, 665, 714, 755
Bso31I GGTCTC 1 cut(s) 842
BsoBI CYCGRG 5 cut(s) 41, 209, 225, 498, 750
Bsp120I GGGCCC 3 cut(s) 228, 271, 753
Bsp1286I GDGCHC 4 cut(s) 232, 275, 751, 757
Bsp13I TCCGGA 2 cut(s) 128, 721
Bsp143I GATC 4 cut(s) 14, 670, 681, 718
BspACI CCGC 9 cut(s) 102, 204, 269, 307, 461, 744, 767, 774, 834
BspANI GGCC 5 cut(s) 230, 273, 665, 714, 755
BspCNI CTCAG 1 cut(s) 197
BspEI TCCGGA 2 cut(s) 128, 721
BspFNI CGCG 2 cut(s) 744, 769
BspLI GGNNCC 9 cut(s) 123, 230, 231, 273, 274, 530, 531, 605, 755
BspMI ACCTGC 1 cut(s) 503
BspPI GGATC 2 cut(s) 9, 713
BspT107I GGYRCC 2 cut(s) 121, 603
BspTI CTTAAG 1 cut(s) 877
BspTNI GGTCTC 1 cut(s) 842
BssECI CCNNGG 7 cut(s) 225, 354, 498, 499, 636, 777, 829
BssMI GATC 4 cut(s) 14, 670, 681, 718
BssNI GRCGYC 3 cut(s) 163, 411, 604
Bst2UI CCWGG 1 cut(s) 266
BstACI GRCGYC 3 cut(s) 163, 411, 604
BstAFI CTTAAG 1 cut(s) 877
BstC8I GCNNGC 2 cut(s) 271, 712
BstDEI CTNAG 3 cut(s) 8, 184, 793
BstDSI CCRYGG 1 cut(s) 777
BstENI CCTNNNNNAGG 1 cut(s) 815
BstF5I GGATG 4 cut(s) 125, 143, 215, 568
BstFNI CGCG 2 cut(s) 744, 769
BstH2I RGCGCY 1 cut(s) 607
BstHHI GCGC 3 cut(s) 143, 606, 744
BstKTI GATC 4 cut(s) 17, 673, 684, 721
BstMAI GTCTC 1 cut(s) 842
BstMBI GATC 4 cut(s) 14, 670, 681, 718
BstMCI CGRYCG 1 cut(s) 492
BstMWI GCNNNNNNNGC 2 cut(s) 140, 906
BstNI CCWGG 1 cut(s) 266
BstNSI RCATGY 1 cut(s) 916
BstSCI CCNGG 7 cut(s) 224, 225, 264, 497, 498, 558, 828
BstSFI CTRYAG 1 cut(s) 105
BstSLI GKGCMC 3 cut(s) 232, 275, 757
BstUI CGCG 2 cut(s) 744, 769
BstX2I RGATCY 2 cut(s) 14, 670
BstYI RGATCY 2 cut(s) 14, 670
BsuRI GGCC 5 cut(s) 230, 273, 665, 714, 755
BtgI CCRYGG 1 cut(s) 777
BtrI CACGTC 1 cut(s) 304
BtsCI GGATG 4 cut(s) 125, 143, 215, 568
BveI ACCTGC 1 cut(s) 503
Cac8I GCNNGC 2 cut(s) 271, 712
CfoI GCGC 3 cut(s) 143, 606, 744
Cfr9I CCCGGG 2 cut(s) 225, 498
CseI GACGC 1 cut(s) 152
Csp6I GTAC 2 cut(s) 698, 771
CviAII CATG 2 cut(s) 115, 913
CviQI GTAC 2 cut(s) 698, 771
DdeI CTNAG 3 cut(s) 8, 184, 793
DinI GGCGCC 1 cut(s) 605
DpnI GATC 4 cut(s) 16, 672, 683, 720
DpnII GATC 4 cut(s) 14, 670, 681, 718
EciI GGCGGA 2 cut(s) 193, 849
Eco147I AGGCCT 1 cut(s) 714
Eco24I GRGCYC 4 cut(s) 232, 275, 751, 757
Eco31I GGTCTC 1 cut(s) 842
Eco47I GGWCC 4 cut(s) 262, 515, 529, 820
Eco88I CYCGRG 5 cut(s) 41, 209, 225, 498, 750
EcoNI CCTNNNNNAGG 1 cut(s) 815
EcoO109I RGGNCCY 5 cut(s) 229, 272, 529, 754, 820
EcoRI GAATTC 1 cut(s) 622
EcoRII CCWGG 1 cut(s) 264
EcoT38I GRGCYC 4 cut(s) 232, 275, 751, 757
EgeI GGCGCC 1 cut(s) 605
EheI GGCGCC 1 cut(s) 605
FaeI CATG 2 cut(s) 118, 916
FaiI YATR 8 cut(s) 65, 107, 116, 407, 705, 707, 807, 914
FaqI GGGAC 1 cut(s) 515
FatI CATG 2 cut(s) 114, 912
FauI CCCGC 4 cut(s) 109, 262, 300, 737
FauNDI CATATG 1 cut(s) 705
FblI GTMKAC 1 cut(s) 37
Fnu4HI GCNGC 2 cut(s) 461, 910
FokI GGATG 4 cut(s) 112, 130, 202, 575
FriOI GRGCYC 4 cut(s) 232, 275, 751, 757
Fsp4HI GCNGC 2 cut(s) 461, 910
GlaI GCGC 3 cut(s) 142, 605, 743
GluI GCNGC 2 cut(s) 461, 910
GsaI CCCAGC 2 cut(s) 460, 740
GsuI CTGGAG 1 cut(s) 836
HaeII RGCGCY 1 cut(s) 607
HaeIII GGCC 5 cut(s) 230, 273, 665, 714, 755
HapII CCGG 8 cut(s) 129, 138, 226, 488, 499, 560, 722, 829
HgaI GACGC 1 cut(s) 152
HhaI GCGC 3 cut(s) 143, 606, 744
Hin1I GRCGYC 3 cut(s) 163, 411, 604
Hin1II CATG 2 cut(s) 118, 916
Hin6I GCGC 3 cut(s) 141, 604, 742
HinP1I GCGC 3 cut(s) 141, 604, 742
HincII GTYRAC 1 cut(s) 38
HindII GTYRAC 1 cut(s) 38
HinfI GANTC 8 cut(s) 39, 95, 200, 293, 422, 581, 801, 837
HpaII CCGG 8 cut(s) 129, 138, 226, 488, 499, 560, 722, 829
HphI GGTGA 2 cut(s) 90, 548
Hpy166II GTNNAC 1 cut(s) 38
Hpy188I TCNGA 3 cut(s) 187, 337, 847
Hpy188III TCNNGA 5 cut(s) 129, 250, 679, 722, 815
Hpy8I GTNNAC 1 cut(s) 38
Hpy99I CGWCG 1 cut(s) 905
HpyAV CCTTC 6 cut(s) 28, 188, 434, 719, 821, 862
HpyCH4IV ACGT 2 cut(s) 303, 411
HpyF10VI GCNNNNNNNGC 2 cut(s) 140, 906
HpyF3I CTNAG 3 cut(s) 8, 184, 793
HpySE526I ACGT 2 cut(s) 303, 411
Hsp92I GRCGYC 3 cut(s) 163, 411, 604
Hsp92II CATG 2 cut(s) 118, 916
HspAI GCGC 3 cut(s) 141, 604, 742
KasI GGCGCC 1 cut(s) 603
KflI GGGWCCC 1 cut(s) 529
Kpn2I TCCGGA 2 cut(s) 128, 721
Kzo9I GATC 4 cut(s) 14, 670, 681, 718
LmnI GCTCC 1 cut(s) 131
LweI GCATC 2 cut(s) 79, 152
MaeII ACGT 2 cut(s) 303, 411
MalI GATC 4 cut(s) 16, 672, 683, 720
MboI GATC 4 cut(s) 14, 670, 681, 718
MboII GAAGA 4 cut(s) 24, 458, 551, 680
MflI RGATCY 2 cut(s) 14, 670
MhlI GDGCHC 4 cut(s) 232, 275, 751, 757
MluCI AATT 7 cut(s) 157, 523, 545, 622, 647, 657, 693
Mly113I GGCGCC 1 cut(s) 604
MlyI GAGTC 3 cut(s) 33, 795, 846
MmeI TCCRAC 2 cut(s) 171, 371
MroI TCCGGA 2 cut(s) 128, 721
MseI TTAA 2 cut(s) 863, 878
MslI CAYNNNNRTG 1 cut(s) 561
MspCI CTTAAG 1 cut(s) 877
MspI CCGG 8 cut(s) 129, 138, 226, 488, 499, 560, 722, 829
MspR9I CCNGG 7 cut(s) 226, 227, 266, 499, 500, 560, 830
MvaI CCWGG 1 cut(s) 266
MvnI CGCG 2 cut(s) 744, 769
MwoI GCNNNNNNNGC 2 cut(s) 140, 906
NarI GGCGCC 1 cut(s) 604
NciI CCSGG 6 cut(s) 226, 227, 499, 500, 560, 830
NdeI CATATG 1 cut(s) 705
NdeII GATC 4 cut(s) 14, 670, 681, 718
NlaIII CATG 2 cut(s) 118, 916
NlaIV GGNNCC 9 cut(s) 123, 230, 231, 273, 274, 530, 531, 605, 755
NmeAIII GCCGAG 1 cut(s) 661
NspI RCATGY 1 cut(s) 916
PceI AGGCCT 1 cut(s) 714
PcsI WCGNNNNNNNCGW 2 cut(s) 293, 408
PfeI GAWTC 5 cut(s) 95, 200, 293, 422, 581
PkrI GCNGC 2 cut(s) 462, 911
PleI GAGTC 3 cut(s) 33, 795, 845
PluTI GGCGCC 1 cut(s) 607
PpsI GAGTC 3 cut(s) 33, 795, 845
PpuMI RGGWCCY 2 cut(s) 529, 820
Psp5II RGGWCCY 2 cut(s) 529, 820
Psp6I CCWGG 1 cut(s) 264
PspFI CCCAGC 2 cut(s) 456, 736
PspGI CCWGG 1 cut(s) 264
PspN4I GGNNCC 9 cut(s) 123, 230, 231, 273, 274, 530, 531, 605, 755
PspOMI GGGCCC 3 cut(s) 228, 271, 753
PspPPI RGGWCCY 2 cut(s) 529, 820
PsuI RGATCY 2 cut(s) 14, 670
RsaI GTAC 2 cut(s) 699, 772
RsaNI GTAC 2 cut(s) 698, 771
RseI CAYNNNNRTG 1 cut(s) 561
SalI GTCGAC 1 cut(s) 36
SaqAI TTAA 2 cut(s) 863, 878
SatI GCNGC 2 cut(s) 461, 910
Sau3AI GATC 4 cut(s) 14, 670, 681, 718
SchI GAGTC 3 cut(s) 33, 795, 846
ScrFI CCNGG 7 cut(s) 226, 227, 266, 499, 500, 560, 830
SduI GDGCHC 4 cut(s) 232, 275, 751, 757
SfaNI GCATC 2 cut(s) 79, 152
SfcI CTRYAG 1 cut(s) 105
SfoI GGCGCC 1 cut(s) 605
SinI GGWCC 4 cut(s) 262, 515, 529, 820
SmaI CCCGGG 2 cut(s) 227, 500
SmiMI CAYNNNNRTG 1 cut(s) 561
SmlI CTYRAG 1 cut(s) 877
SmoI CTYRAG 1 cut(s) 877
SrfI GCCCGGGC 1 cut(s) 227
Sse9I AATT 7 cut(s) 157, 523, 545, 622, 647, 657, 693
SseBI AGGCCT 1 cut(s) 714
SsiI CCGC 9 cut(s) 102, 204, 269, 307, 461, 744, 767, 774, 834
SspDI GGCGCC 1 cut(s) 603
StuI AGGCCT 1 cut(s) 714
StyD4I CCNGG 7 cut(s) 224, 225, 264, 497, 498, 558, 828
TaiI ACGT 2 cut(s) 306, 414
TaqI TCGA 6 cut(s) 21, 37, 402, 593, 678, 759
TasI AATT 7 cut(s) 157, 523, 545, 622, 647, 657, 693
TauI GCSGC 1 cut(s) 463
TfiI GAWTC 5 cut(s) 95, 200, 293, 422, 581
Tru1I TTAA 2 cut(s) 863, 878
Tru9I TTAA 2 cut(s) 863, 878
TseI GCWGC 1 cut(s) 909
TspDTI ATGAA 1 cut(s) 579
TspGWI ACGGA 2 cut(s) 302, 507
TspMI CCCGGG 2 cut(s) 225, 498
Vha464I CTTAAG 1 cut(s) 877
VpaK11BI GGWCC 4 cut(s) 262, 515, 529, 820
XagI CCTNNNNNAGG 1 cut(s) 815
XapI RAATTY 3 cut(s) 545, 622, 647
XceI RCATGY 1 cut(s) 916
XmaI CCCGGG 2 cut(s) 225, 498
XmiI GTMKAC 1 cut(s) 37
ZraI GACGTC 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.