pycom461g00340

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
tig00000461
OG0018036
Physical Location & Seq
Reverse (-)
274699 .. 275001
303 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 303 bp
ATGGAAGCTAAATCCGTTAGGATCATCAGAACGCATCGAGACTTTTATCTCCAAAAAATCGTTGAATACCTAAGTTCTGCACGCATTGCAGACGACAAGGTCATGCCCGTCACGAATGCAAAGTGGCGAATAAGGAACCTGGTGTCAACCCGTCACCGAGAATGGCCTATGTTCCAAGAACCAATGCTACCAATACGGTTAGCACCTACCACTGAAACGATTCCTACTGCTGATAATGATGCGGCTACTGTTCAAAATGCACCTGCTGAGGTGAATGCTGGTGTGGCTGAAAGAATTCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

101

Amino Acids

11.49

Weight (kDa)

9.68

Isoelectric Point (pI)

29.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018036)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g07761 FvH4_5g07762 FvH4_5g07800
pyrus_communis pycom461g00020 pycom461g00340
rosa_laevigata RLG00000000445 RLG00000000453 RLG00000006086
rosa_rugosa RorugMtG0000100.1
rosa_samantha Rh4AG240000 Rh4BG243200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 271
AasI GACNNNNNNGTC 1 cut(s) 98
Acc36I ACCTGC 1 cut(s) 271
AciI CCGC 1 cut(s) 242
AclWI GGATC 1 cut(s) 29
AcsI RAATTY 1 cut(s) 294
AgsI TTSAA 2 cut(s) 65, 254
AjnI CCWGG 1 cut(s) 138
AluBI AGCT 1 cut(s) 8
AluI AGCT 1 cut(s) 8
Alw26I GTCTC 1 cut(s) 33
AlwI GGATC 1 cut(s) 29
AoxI GGCC 1 cut(s) 164
ApoI RAATTY 1 cut(s) 294
Asp700I GAANNNNTTC 2 cut(s) 219, 294
AsuHPI GGTGA 2 cut(s) 146, 283
BbvCI CCTCAGC 1 cut(s) 267
BciT130I CCWGG 1 cut(s) 140
BcoDI GTCTC 1 cut(s) 33
BfuAI ACCTGC 1 cut(s) 271
BisI GCNGC 1 cut(s) 243
BlsI GCNGC 1 cut(s) 244
Bme1390I CCNGG 1 cut(s) 140
BmiI GGNNCC 1 cut(s) 137
BmrFI CCNGG 1 cut(s) 140
BmsI GCATC 2 cut(s) 43, 229
Bpu10I CCTNAGC 1 cut(s) 267
Bse3DI GCAATG 1 cut(s) 84
BseBI CCWGG 1 cut(s) 140
BseMI GCAATG 1 cut(s) 84
BseMII CTCAG 1 cut(s) 258
BsgI GTGCAG 1 cut(s) 63
BshFI GGCC 1 cut(s) 166
BsmAI GTCTC 1 cut(s) 33
BsmI GAATGC 2 cut(s) 121, 280
BsnI GGCC 1 cut(s) 166
Bsp143I GATC 1 cut(s) 21
BspACI CCGC 1 cut(s) 242
BspANI GGCC 1 cut(s) 166
BspCNI CTCAG 1 cut(s) 259
BspLI GGNNCC 1 cut(s) 137
BspMI ACCTGC 1 cut(s) 271
BspPI GGATC 1 cut(s) 29
BsrDI GCAATG 1 cut(s) 84
BssMI GATC 1 cut(s) 21
Bst2UI CCWGG 1 cut(s) 140
Bst4CI ACNGT 2 cut(s) 198, 250
BstAPI GCANNNNNTGC 1 cut(s) 86
BstC8I GCNNGC 1 cut(s) 82
BstDEI CTNAG 2 cut(s) 71, 267
BstKTI GATC 1 cut(s) 24
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 1 cut(s) 21
BstMWI GCNNNNNNNGC 2 cut(s) 86, 284
BstNI CCWGG 1 cut(s) 140
BstSCI CCNGG 1 cut(s) 138
BsuRI GGCC 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 210
BveI ACCTGC 1 cut(s) 271
Cac8I GCNNGC 1 cut(s) 82
CsiI ACCWGGT 1 cut(s) 138
CviAII CATG 1 cut(s) 103
CviJI RGCY 4 cut(s) 8, 166, 245, 287
CviKI_1 RGCY 4 cut(s) 8, 166, 245, 287
DdeI CTNAG 2 cut(s) 71, 267
DpnI GATC 1 cut(s) 23
DpnII GATC 1 cut(s) 21
DrdI GACNNNNNNGTC 1 cut(s) 98
DseDI GACNNNNNNGTC 1 cut(s) 98
EcoRI GAATTC 1 cut(s) 294
EcoRII CCWGG 1 cut(s) 138
FaeI CATG 1 cut(s) 106
FaiI YATR 2 cut(s) 104, 170
FatI CATG 1 cut(s) 102
Fnu4HI GCNGC 1 cut(s) 243
Fsp4HI GCNGC 1 cut(s) 243
GluI GCNGC 1 cut(s) 243
HaeIII GGCC 1 cut(s) 166
Hin1II CATG 1 cut(s) 106
HincII GTYRAC 1 cut(s) 147
HindII GTYRAC 1 cut(s) 147
HinfI GANTC 1 cut(s) 220
HphI GGTGA 2 cut(s) 146, 283
Hpy166II GTNNAC 1 cut(s) 147
Hpy188I TCNGA 1 cut(s) 29
Hpy188III TCNNGA 2 cut(s) 38, 112
Hpy8I GTNNAC 1 cut(s) 147
HpyCH4III ACNGT 2 cut(s) 198, 250
HpyCH4V TGCA 4 cut(s) 80, 89, 119, 260
HpyF10VI GCNNNNNNNGC 2 cut(s) 86, 284
HpyF3I CTNAG 2 cut(s) 71, 267
Hsp92II CATG 1 cut(s) 106
Kzo9I GATC 1 cut(s) 21
LpnPI CCDG 4 cut(s) 125, 152, 264, 276
LweI GCATC 2 cut(s) 43, 229
MabI ACCWGGT 1 cut(s) 138
MaeIII GTNAC 2 cut(s) 109, 152
MalI GATC 1 cut(s) 23
MboI GATC 1 cut(s) 21
MluCI AATT 1 cut(s) 294
MnlI CCTC 1 cut(s) 262
MroXI GAANNNNTTC 2 cut(s) 219, 294
MspR9I CCNGG 1 cut(s) 140
Mva1269I GAATGC 2 cut(s) 121, 280
MvaI CCWGG 1 cut(s) 140
MwoI GCNNNNNNNGC 2 cut(s) 86, 284
NdeII GATC 1 cut(s) 21
NlaIII CATG 1 cut(s) 106
NlaIV GGNNCC 1 cut(s) 137
NmuCI GTSAC 2 cut(s) 109, 152
PaqCI CACCTGC 1 cut(s) 271
PctI GAATGC 2 cut(s) 121, 280
PdmI GAANNNNTTC 2 cut(s) 219, 294
PfeI GAWTC 1 cut(s) 220
PflFI GACNNNGTC 1 cut(s) 98
PkrI GCNGC 1 cut(s) 244
Psp6I CCWGG 1 cut(s) 138
PspGI CCWGG 1 cut(s) 138
PspN4I GGNNCC 1 cut(s) 137
PsrI GAACNNNNNNTAC 2 cut(s) 171, 203
PsyI GACNNNGTC 1 cut(s) 98
SatI GCNGC 1 cut(s) 243
Sau3AI GATC 1 cut(s) 21
ScrFI CCNGG 1 cut(s) 140
SetI ASST 7 cut(s) 10, 72, 102, 141, 208, 265, 273
SexAI ACCWGGT 1 cut(s) 138
SfaNI GCATC 2 cut(s) 43, 229
Sse9I AATT 1 cut(s) 294
SsiI CCGC 1 cut(s) 242
StyD4I CCNGG 1 cut(s) 138
TaaI ACNGT 2 cut(s) 198, 250
TaqI TCGA 1 cut(s) 37
TasI AATT 1 cut(s) 294
TauI GCSGC 1 cut(s) 245
TfiI GAWTC 1 cut(s) 220
TscAI CASTG 1 cut(s) 217
TseFI GTSAC 2 cut(s) 109, 152
Tsp45I GTSAC 2 cut(s) 109, 152
TspGWI ACGGA 1 cut(s) 4
TspRI CASTG 1 cut(s) 217
Tth111I GACNNNGTC 1 cut(s) 98
XapI RAATTY 1 cut(s) 294
XmnI GAANNNNTTC 2 cut(s) 219, 294
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.