RchiOBHm_Chr6g0291111

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0013302
Physical Location & Seq
Forward (+)
54309279 .. 54311386
2108 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1527 bp
ATGAATAATGACATGTTGCTAAGAAACCTGCGAAGCTCCATCGCCGCCGTCCGCCGTTTCTCGGCGGTGGCGAGCACCGGAGCGACGAAGGAATCGGGCGGTGGAAGAGACACGCTGGGAAGGAGACTTCTGGGCCTGGTGTATGCAAAACGCAGCGCGGCGGTTACTATAGGGAAATGGAAAGAGGAAGGAGGCACAGTGCGGAAGTATGAGCTTAATCGAATTGTTCGGGAGCTTCGTAAGCTCCGGCGTTATAAGCACGCTCTTGAGATATGCGAATGGATGACTTTACAGAAAGATGTCAAGCTACTGGCAGGTGATTATGCTGTTCAGTTGGATTTGATTGCAAAGGTTCGTGGTATGAATAGCGCAGAGAAGTTTTTCGAGGATCTCCCTGAGCAGATGAGAGACGGCACGACCTGTTCAGCTCTTCTGCATACATATGTCCAGAATAAAGAGGCTGCCAAAGCGGAAGCTCTGGTGGAGAAGATGAAAGAGTGTGGTTTCGTGAAGAATCCCCTTCCTTACAACCACATGCTGTCTTTGTATGTGTCCGATGGGCAATTAGACAAGATTCCAGAAGTAATTGGGGAGATGAAGGTTCATACTAAACCGGATGTTGTCACTTACAATCTATGGTTAACAGTTTGTGCTTCGCAAAATAATGTTGATAGTGCAGAAAGAGTGTTTCTTGAATTGAAGAAGGCAAAGGTAAATCCGGACTGGGTGACATTTAGCACGCTAACAAACTTGTACATCAAAAGTTCACTCATTGAAAAAGCAGCAGCTACCTTGAAGGAGATGGAGAATATGGCATCTCGGAAGCATCGAGTCGCATATTCATCTCTCCTCAGCCTGCATGCAAACATTGGGGACAAGGCTGGTGTTCAGCGAGTTTGGAAGAAACTAAAATCATGTTTCCCCAAAATGAATGATGCTGAGTATACATGTATGATAGCTTCGCTTGTGAAACTCAAGGAGATTGAAGAAGCTGAGAAACTCTATACTGAGTGGGAGTCTGTTTCTGGGACTCATGATCCTAGGGTCTCGAATATAATTCTTGCAGCTTACATCAACAGAAACCAAATGGATGTGGCTGAAACCTTCTGTAGTCGTATGGTGCAAAATAGCATCACCCCCTGTTACAGTACTTGGGAGCTTCTTACGTGGGGCTATTTGAAACAAAAGCATACGAAAAAAGTGCTAGAGTATTTTAAGAGAGCAGTTGGTAGTGTGAAAAAATGGGATCCTGACCAAAGATTGATTGCAGAAGTATTTGGCCGGCTCAATGAAGAAGGCAGTGTTGAAGAGGCAGAGGAACTAGTGGTTTTCCTTCGGAATGCTGGTCATGTGAGTACTGAGACATATAATTCACTTCTACGAACTTATGCTCAAGCTGGTACAATGCCACTTTTGGTTGAAGAACGGATGAAGAAGGACAATGTTGAATTGAATAAGGAGACTCGCTCGCTTATAAAAAGGATCAACAAAATTTGTGCGAGTGAAGTTCCTAGCCTATGTTCTTAA

Protein Analysis

508

Amino Acids

57.7

Weight (kDa)

9.11

Isoelectric Point (pI)

36.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_3 PF13812 175 - 217 7.3e-06 Pentatricopeptide repeat domain
PPR_3 PF13812 204 - 252 3e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 205 - 250 3.8e-10 PPR repeat family
PPR_long PF17177 214 - 305 3.7e-06 Pentacotripeptide-repeat region of PRORP
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013302)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G02820
fragaria_vesca FvH4_2g23640
malus_domestica MD03G1265900.v1.1
prunus_persica Prupe.8G241600_v2.0.a1 Prupe.8G241600_v2.0.a1 Prupe.8G241600_v2.0.a1
pyrus_communis pycom03g21240
rosa_chinensis RchiOBHm_Chr6g0291111
rosa_laevigata RLG00000012143
rosa_multiflora Rmu_sc0004642.1_g000017 Rmu_sc0033743.1_g000001
rosa_roxburghii Rroxscaffold_7G00176370
rosa_rugosa Rorug06G0217700
rosa_samantha Rh6AG327900 Rh6BG335400 Rh6CG341600 Rh6DG328800
rosa_wichuraiana Rw6G028520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 255, 1475
AarI CACCTGC 1 cut(s) 305
Acc36I ACCTGC 2 cut(s) 36, 305
AccI GTMKAC 1 cut(s) 944
AccII CGCG 1 cut(s) 158
AccIII TCCGGA 1 cut(s) 718
AciI CCGC 8 cut(s) 45, 52, 65, 99, 158, 161, 202, 470
AclWI GGATC 5 cut(s) 396, 1031, 1241, 1254, 1490
AcoI YGGCCR 1 cut(s) 1279
AcsI RAATTY 1 cut(s) 1491
AfaI GTAC 4 cut(s) 755, 1150, 1357, 1402
AfiI CCNNNNNNNGG 1 cut(s) 61
AflIII ACRYGT 2 cut(s) 12, 947
AhlI ACTAGT 1 cut(s) 1321
AjnI CCWGG 1 cut(s) 135
Alw21I GWGCWC 1 cut(s) 77
Alw26I GTCTC 6 cut(s) 102, 118, 402, 1051, 1355, 1454
AlwI GGATC 5 cut(s) 396, 1031, 1241, 1254, 1490
Aor13HI TCCGGA 1 cut(s) 718
AoxI GGCC 2 cut(s) 133, 1279
ApeKI GCWGC 5 cut(s) 153, 461, 782, 785, 1064
ApoI RAATTY 1 cut(s) 1491
Asp700I GAANNNNTTC 1 cut(s) 380
AspA2I CCTAGG 1 cut(s) 1040
AspLEI GCGC 2 cut(s) 158, 371
AspS9I GGNCC 1 cut(s) 133
AsuHPI GGTGA 3 cut(s) 329, 739, 1126
AvrII CCTAGG 1 cut(s) 1040
BamHI GGATCC 1 cut(s) 1246
BarI GAAGNNNNNNTAC 2 cut(s) 943, 975
Bbv12I GWGCWC 1 cut(s) 77
BbvCI CCTCAGC 1 cut(s) 851
BbvI GCAGC 5 cut(s) 165, 448, 794, 797, 1076
BccI CCATC 3 cut(s) 47, 551, 796
BceAI ACGGC 3 cut(s) 32, 39, 427
BcgI CGANNNNNNTGC 2 cut(s) 1183, 1217
BciT130I CCWGG 1 cut(s) 137
BcoDI GTCTC 6 cut(s) 102, 118, 402, 1051, 1355, 1454
BcuI ACTAGT 1 cut(s) 1321
BfaI CTAG 4 cut(s) 1041, 1205, 1322, 1512
BfmI CTRYAG 2 cut(s) 168, 1108
BfuAI ACCTGC 2 cut(s) 36, 305
BisI GCNGC 7 cut(s) 45, 154, 159, 462, 783, 786, 1065
BlnI CCTAGG 1 cut(s) 1040
BlsI GCNGC 7 cut(s) 46, 155, 160, 463, 784, 787, 1066
BmcAI AGTACT 2 cut(s) 1150, 1357
Bme1390I CCNGG 1 cut(s) 137
BmgT120I GGNCC 1 cut(s) 133
BmiI GGNNCC 1 cut(s) 1248
BmrFI CCNGG 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 733
BmsI GCATC 4 cut(s) 824, 835, 925, 1140
BmuI ACTGGG 1 cut(s) 733
BplI GAGNNNNNCTC 2 cut(s) 1451, 1483
Bpu10I CCTNAGC 2 cut(s) 396, 851
BpuEI CTTGAG 3 cut(s) 287, 959, 1377
BsaAI YACGTR 1 cut(s) 1167
BsaI GGTCTC 1 cut(s) 1051
BsaJI CCNNGG 1 cut(s) 1040
BsaWI WCCGGW 3 cut(s) 77, 613, 718
BsaXI ACNNNNNCTCC 2 cut(s) 183, 213
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse118I RCCGGY 1 cut(s) 1281
Bse1I ACTGG 2 cut(s) 315, 728
BseAI TCCGGA 1 cut(s) 718
BseBI CCWGG 1 cut(s) 137
BseDI CCNNGG 1 cut(s) 1040
BseGI GGATG 4 cut(s) 288, 622, 1096, 1434
BseLI CCNNNNNNNGG 1 cut(s) 61
BseMII CTCAG 6 cut(s) 387, 865, 930, 984, 999, 1350
BseNI ACTGG 2 cut(s) 315, 728
BseRI GAGGAG 1 cut(s) 839
BseXI GCAGC 5 cut(s) 165, 448, 794, 797, 1076
BseYI CCCAGC 1 cut(s) 115
BsgI GTGCAG 1 cut(s) 696
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 2 cut(s) 135, 1281
BsiHKAI GWGCWC 1 cut(s) 77
BsiSI CCGG 5 cut(s) 78, 247, 614, 719, 1282
BslFI GGGAC 2 cut(s) 887, 1042
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 6 cut(s) 102, 118, 402, 1051, 1355, 1454
BsmBI CGTCTC 1 cut(s) 402
BsmFI GGGAC 2 cut(s) 887, 1042
BsmI GAATGC 1 cut(s) 1345
BsnI GGCC 2 cut(s) 135, 1281
Bso31I GGTCTC 1 cut(s) 1051
Bsp1286I GDGCHC 1 cut(s) 77
Bsp13I TCCGGA 1 cut(s) 718
Bsp1407I TGTACA 1 cut(s) 753
Bsp143I GATC 4 cut(s) 388, 1036, 1246, 1482
BspACI CCGC 8 cut(s) 45, 52, 65, 99, 158, 161, 202, 470
BspANI GGCC 2 cut(s) 135, 1281
BspCNI CTCAG 6 cut(s) 388, 864, 931, 985, 1000, 1351
BspEI TCCGGA 1 cut(s) 718
BspFNI CGCG 1 cut(s) 158
BspHI TCATGA 1 cut(s) 1033
BspLI GGNNCC 1 cut(s) 1248
BspMI ACCTGC 2 cut(s) 36, 305
BspPI GGATC 5 cut(s) 396, 1031, 1241, 1254, 1490
BspQI GCTCTTC 1 cut(s) 435
BspTNI GGTCTC 1 cut(s) 1051
BsrFI RCCGGY 1 cut(s) 1281
BsrGI TGTACA 1 cut(s) 753
BsrI ACTGG 2 cut(s) 315, 728
BssAI RCCGGY 1 cut(s) 1281
BssECI CCNNGG 1 cut(s) 1040
BssMI GATC 4 cut(s) 388, 1036, 1246, 1482
BssNAI GTATAC 1 cut(s) 945
BssT1I CCWWGG 1 cut(s) 1040
Bst1107I GTATAC 1 cut(s) 945
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 3 cut(s) 199, 646, 1148
Bst6I CTCTTC 3 cut(s) 100, 435, 1302
BstAUI TGTACA 1 cut(s) 753
BstBAI YACGTR 1 cut(s) 1167
BstC8I GCNNGC 7 cut(s) 73, 261, 740, 857, 861, 1283, 1469
BstDEI CTNAG 7 cut(s) 20, 396, 851, 939, 993, 1008, 1359
BstF5I GGATG 4 cut(s) 288, 622, 1096, 1434
BstFNI CGCG 1 cut(s) 158
BstHHI GCGC 2 cut(s) 158, 371
BstKTI GATC 4 cut(s) 391, 1039, 1249, 1485
BstMAI GTCTC 6 cut(s) 102, 118, 402, 1051, 1355, 1454
BstMBI GATC 4 cut(s) 388, 1036, 1246, 1482
BstMWI GCNNNNNNNGC 3 cut(s) 241, 256, 467
BstNI CCWGG 1 cut(s) 137
BstNSI RCATGY 4 cut(s) 16, 538, 863, 951
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 2 cut(s) 168, 1108
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 5 cut(s) 165, 448, 794, 797, 1076
BstX2I RGATCY 2 cut(s) 388, 1246
BstYI RGATCY 2 cut(s) 388, 1246
BstZ17I GTATAC 1 cut(s) 945
BsuRI GGCC 2 cut(s) 135, 1281
BtgZI GCGATG 1 cut(s) 25
BtsCI GGATG 4 cut(s) 288, 622, 1096, 1434
BtsI GCAGTG 1 cut(s) 1306
BtsIMutI CAGTG 2 cut(s) 204, 1306
BveI ACCTGC 2 cut(s) 36, 305
Cac8I GCNNGC 7 cut(s) 73, 261, 740, 857, 861, 1283, 1469
CciI TCATGA 1 cut(s) 1033
CfoI GCGC 2 cut(s) 158, 371
Cfr10I RCCGGY 1 cut(s) 1281
Cfr13I GGNCC 1 cut(s) 133
Csp6I GTAC 4 cut(s) 754, 1149, 1356, 1401
CviAII CATG 7 cut(s) 13, 535, 860, 915, 948, 1034, 1349
CviQI GTAC 4 cut(s) 754, 1149, 1356, 1401
DdeI CTNAG 7 cut(s) 20, 396, 851, 939, 993, 1008, 1359
DpnI GATC 4 cut(s) 390, 1038, 1248, 1484
DpnII GATC 4 cut(s) 388, 1036, 1246, 1482
EaeI YGGCCR 1 cut(s) 1279
Eam1104I CTCTTC 3 cut(s) 100, 435, 1302
EarI CTCTTC 3 cut(s) 100, 435, 1302
EciI GGCGGA 1 cut(s) 41
Eco130I CCWWGG 1 cut(s) 1040
Eco31I GGTCTC 1 cut(s) 1051
EcoRII CCWGG 1 cut(s) 135
EcoT14I CCWWGG 1 cut(s) 1040
ErhI CCWWGG 1 cut(s) 1040
Esp3I CGTCTC 1 cut(s) 402
FaeI CATG 7 cut(s) 16, 538, 863, 918, 951, 1037, 1352
FaqI GGGAC 2 cut(s) 887, 1042
FatI CATG 7 cut(s) 12, 534, 859, 914, 947, 1033, 1348
FauNDI CATATG 1 cut(s) 442
FblI GTMKAC 1 cut(s) 944
Fnu4HI GCNGC 7 cut(s) 45, 154, 159, 462, 783, 786, 1065
FokI GGATG 4 cut(s) 295, 629, 1103, 1441
Fsp4HI GCNGC 7 cut(s) 45, 154, 159, 462, 783, 786, 1065
FspBI CTAG 4 cut(s) 1041, 1205, 1322, 1512
GlaI GCGC 2 cut(s) 157, 370
GluI GCNGC 7 cut(s) 45, 154, 159, 462, 783, 786, 1065
GsaI CCCAGC 1 cut(s) 119
HaeIII GGCC 2 cut(s) 135, 1281
HapII CCGG 5 cut(s) 78, 247, 614, 719, 1282
HhaI GCGC 2 cut(s) 158, 371
Hin1II CATG 7 cut(s) 16, 538, 863, 918, 951, 1037, 1352
Hin6I GCGC 2 cut(s) 156, 369
HinP1I GCGC 2 cut(s) 156, 369
HincII GTYRAC 1 cut(s) 642
HindII GTYRAC 1 cut(s) 642
HinfI GANTC 7 cut(s) 92, 514, 574, 831, 1016, 1030, 1462
HpaI GTTAAC 1 cut(s) 642
HpaII CCGG 5 cut(s) 78, 247, 614, 719, 1282
HphI GGTGA 3 cut(s) 329, 739, 1126
Hpy166II GTNNAC 3 cut(s) 642, 767, 945
Hpy188I TCNGA 3 cut(s) 556, 822, 1338
Hpy8I GTNNAC 3 cut(s) 642, 767, 945
Hpy99I CGWCG 1 cut(s) 88
HpyCH4III ACNGT 3 cut(s) 199, 646, 1148
HpyCH4IV ACGT 1 cut(s) 1166
HpyCH4V TGCA 9 cut(s) 146, 347, 436, 677, 859, 863, 1064, 1123, 1268
HpyF10VI GCNNNNNNNGC 3 cut(s) 241, 256, 467
HpyF3I CTNAG 7 cut(s) 20, 396, 851, 939, 993, 1008, 1359
HpySE526I ACGT 1 cut(s) 1166
Hsp92II CATG 7 cut(s) 16, 538, 863, 918, 951, 1037, 1352
HspAI GCGC 2 cut(s) 156, 369
Kpn2I TCCGGA 1 cut(s) 718
KroI GCCGGC 1 cut(s) 1281
KroNI GCCGGC 1 cut(s) 1283
KspAI GTTAAC 1 cut(s) 642
Kzo9I GATC 4 cut(s) 388, 1036, 1246, 1482
LguI GCTCTTC 1 cut(s) 435
LmnI GCTCC 5 cut(s) 41, 80, 232, 249, 1156
Lsp1109I GCAGC 5 cut(s) 165, 448, 794, 797, 1076
LweI GCATC 4 cut(s) 824, 835, 925, 1140
MaeI CTAG 4 cut(s) 1041, 1205, 1322, 1512
MaeII ACGT 1 cut(s) 1166
MaeIII GTNAC 4 cut(s) 163, 622, 727, 1142
MalI GATC 4 cut(s) 390, 1038, 1248, 1484
MboI GATC 4 cut(s) 388, 1036, 1246, 1482
MflI RGATCY 2 cut(s) 388, 1246
MhlI GDGCHC 1 cut(s) 77
MluCI AATT 8 cut(s) 222, 563, 585, 695, 1056, 1369, 1448, 1491
MlyI GAGTC 4 cut(s) 840, 1024, 1025, 1456
MmeI TCCRAC 1 cut(s) 315
MnlI CCTC 7 cut(s) 178, 185, 379, 451, 860, 1303, 1309
MroI TCCGGA 1 cut(s) 718
MroNI GCCGGC 1 cut(s) 1281
MroXI GAANNNNTTC 1 cut(s) 380
MseI TTAA 4 cut(s) 216, 641, 1215, 1525
MslI CAYNNNNRTG 1 cut(s) 441
MspI CCGG 5 cut(s) 78, 247, 614, 719, 1282
MspR9I CCNGG 1 cut(s) 137
Mva1269I GAATGC 1 cut(s) 1345
MvaI CCWGG 1 cut(s) 137
MvnI CGCG 1 cut(s) 158
MwoI GCNNNNNNNGC 3 cut(s) 241, 256, 467
NaeI GCCGGC 1 cut(s) 1283
NdeI CATATG 1 cut(s) 442
NdeII GATC 4 cut(s) 388, 1036, 1246, 1482
NgoMIV GCCGGC 1 cut(s) 1281
NlaIII CATG 7 cut(s) 16, 538, 863, 918, 951, 1037, 1352
NlaIV GGNNCC 1 cut(s) 1248
NmeAIII GCCGAG 1 cut(s) 41
NmuCI GTSAC 2 cut(s) 622, 727
NspI RCATGY 4 cut(s) 16, 538, 863, 951
PaeI GCATGC 1 cut(s) 863
PagI TCATGA 1 cut(s) 1033
PaqCI CACCTGC 1 cut(s) 305
PciI ACATGT 2 cut(s) 12, 947
PciSI GCTCTTC 1 cut(s) 435
PcsI WCGNNNNNNNCGW 3 cut(s) 68, 235, 826
PctI GAATGC 1 cut(s) 1345
PdiI GCCGGC 1 cut(s) 1283
PdmI GAANNNNTTC 1 cut(s) 380
PfeI GAWTC 3 cut(s) 92, 514, 574
PkrI GCNGC 7 cut(s) 46, 155, 160, 463, 784, 787, 1066
PleI GAGTC 4 cut(s) 839, 1024, 1024, 1456
PpsI GAGTC 4 cut(s) 839, 1024, 1024, 1456
Ppu21I YACGTR 1 cut(s) 1167
PscI ACATGT 2 cut(s) 12, 947
PsiI TTATAA 2 cut(s) 255, 1475
Psp6I CCWGG 1 cut(s) 135
PspFI CCCAGC 1 cut(s) 115
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 1 cut(s) 1248
PspPI GGNCC 1 cut(s) 133
PsuI RGATCY 2 cut(s) 388, 1246
RsaI GTAC 4 cut(s) 755, 1150, 1357, 1402
RsaNI GTAC 4 cut(s) 754, 1149, 1356, 1401
RseI CAYNNNNRTG 1 cut(s) 441
SapI GCTCTTC 1 cut(s) 435
SaqAI TTAA 4 cut(s) 216, 641, 1215, 1525
SatI GCNGC 7 cut(s) 45, 154, 159, 462, 783, 786, 1065
Sau3AI GATC 4 cut(s) 388, 1036, 1246, 1482
Sau96I GGNCC 1 cut(s) 133
ScaI AGTACT 2 cut(s) 1150, 1357
SchI GAGTC 4 cut(s) 840, 1024, 1025, 1456
ScrFI CCNGG 1 cut(s) 137
SduI GDGCHC 1 cut(s) 77
SfaNI GCATC 4 cut(s) 824, 835, 925, 1140
SfcI CTRYAG 2 cut(s) 168, 1108
SmiMI CAYNNNNRTG 1 cut(s) 441
SmlI CTYRAG 3 cut(s) 266, 974, 1392
SmoI CTYRAG 3 cut(s) 266, 974, 1392
SpeI ACTAGT 1 cut(s) 1321
SphI GCATGC 1 cut(s) 863
Sse9I AATT 8 cut(s) 222, 563, 585, 695, 1056, 1369, 1448, 1491
SsiI CCGC 8 cut(s) 45, 52, 65, 99, 158, 161, 202, 470
SspMI CTAG 4 cut(s) 1041, 1205, 1322, 1512
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 1 cut(s) 1040
TaaI ACNGT 3 cut(s) 199, 646, 1148
TaiI ACGT 1 cut(s) 1169
TaqI TCGA 4 cut(s) 220, 384, 829, 1049
TasI AATT 8 cut(s) 222, 563, 585, 695, 1056, 1369, 1448, 1491
TatI WGTACW 3 cut(s) 753, 1148, 1355
TauI GCSGC 2 cut(s) 47, 161
TfiI GAWTC 3 cut(s) 92, 514, 574
Tru1I TTAA 4 cut(s) 216, 641, 1215, 1525
Tru9I TTAA 4 cut(s) 216, 641, 1215, 1525
TscAI CASTG 2 cut(s) 204, 1306
TseFI GTSAC 2 cut(s) 622, 727
TseI GCWGC 5 cut(s) 153, 461, 782, 785, 1064
Tsp45I GTSAC 2 cut(s) 622, 727
TspDTI ATGAA 9 cut(s) 17, 377, 506, 593, 611, 831, 944, 1305, 1445
TspGWI ACGGA 1 cut(s) 1441
TspRI CASTG 2 cut(s) 204, 1306
XapI RAATTY 1 cut(s) 1491
XceI RCATGY 4 cut(s) 16, 538, 863, 951
XmaJI CCTAGG 1 cut(s) 1040
XmiI GTMKAC 1 cut(s) 944
XmnI GAANNNNTTC 1 cut(s) 380
XspI CTAG 4 cut(s) 1041, 1205, 1322, 1512
ZrmI AGTACT 2 cut(s) 1150, 1357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.