RchiOBHm_Chr6g0294031

Outer envelope pore protein 16-4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0011132
Physical Location & Seq
Reverse (-)
56516235 .. 56518800
2566 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 405 bp
ATGGAGGTAGACCCGTACGTCATCGCGCCGTGCTCTTCCCTTGCCGTTTCTTCAATCGTCAACGTCGGAACGGCTGGTGCCATTTGGGGTTTATGCTCCGCTCCCCACGATGCTCATAAATTCGGCCTGACTGGAATAAAACGAGCTGGGTTTGTGGCAAACTCGGTTGCCAGGTGTACCTTTCAATGTGCACTTATTGCTGGAATTTCTACTTCTGTTCACTGTGGAGTGCAAAGATATCGGGGGAAGGATGATTGGGTGAATGGTTTGGTTGCTGGGGCTATGGCAGGGGGAGCTATTGCTGCTCTATCAAGAAGCTGGAAACAGGTGATTCCTACAGCTTGTCTGGTTTCTGCTTTGAAAATTGCCGCCGACTATGGTAGAAAAAATGTGAATACTTCTTGA

Protein Analysis

134

Amino Acids

13.81

Weight (kDa)

9.22

Isoelectric Point (pI)

17.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tim17 PF02466 16 - 118 5.8e-11 Tim17/Tim22/Tim23/Pmp24 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 17
AccB1I GGYRCC 1 cut(s) 77
AccBSI CCGCTC 1 cut(s) 101
AccI GTMKAC 1 cut(s) 9
AccII CGCG 1 cut(s) 26
AciI CCGC 2 cut(s) 99, 369
AcsI RAATTY 2 cut(s) 119, 204
AfaI GTAC 2 cut(s) 17, 178
AgsI TTSAA 3 cut(s) 54, 185, 361
AjnI CCWGG 1 cut(s) 170
AluBI AGCT 4 cut(s) 146, 296, 318, 341
AluI AGCT 4 cut(s) 146, 296, 318, 341
Alw21I GWGCWC 2 cut(s) 35, 193
Alw44I GTGCAC 1 cut(s) 189
AoxI GGCC 1 cut(s) 124
ApaLI GTGCAC 1 cut(s) 189
ApeKI GCWGC 1 cut(s) 302
ApoI RAATTY 2 cut(s) 119, 204
AspLEI GCGC 1 cut(s) 28
AsuHPI GGTGA 2 cut(s) 271, 340
BaeGI GKGCMC 1 cut(s) 193
BanI GGYRCC 1 cut(s) 77
Bbv12I GWGCWC 2 cut(s) 35, 193
BbvI GCAGC 1 cut(s) 289
BceAI ACGGC 3 cut(s) 13, 29, 87
BcgI CGANNNNNNTGC 2 cut(s) 221, 255
BciT130I CCWGG 1 cut(s) 172
BfmI CTRYAG 1 cut(s) 336
BisI GCNGC 2 cut(s) 303, 369
BlsI GCNGC 2 cut(s) 304, 370
Bme1390I CCNGG 1 cut(s) 172
BmiI GGNNCC 1 cut(s) 79
BmrFI CCNGG 1 cut(s) 172
BmsI GCATC 1 cut(s) 100
Bse1I ACTGG 1 cut(s) 136
BseBI CCWGG 1 cut(s) 172
BseGI GGATG 1 cut(s) 256
BseNI ACTGG 1 cut(s) 136
BseSI GKGCMC 1 cut(s) 193
BseXI GCAGC 1 cut(s) 289
BseYI CCCAGC 2 cut(s) 146, 275
Bsh1236I CGCG 1 cut(s) 26
BshFI GGCC 1 cut(s) 126
BshNI GGYRCC 1 cut(s) 77
BsiHKAI GWGCWC 2 cut(s) 35, 193
BsiWI CGTACG 1 cut(s) 15
BsnI GGCC 1 cut(s) 126
Bsp1286I GDGCHC 2 cut(s) 35, 193
BspACI CCGC 2 cut(s) 99, 369
BspANI GGCC 1 cut(s) 126
BspFNI CGCG 1 cut(s) 26
BspLI GGNNCC 1 cut(s) 79
BspQI GCTCTTC 1 cut(s) 40
BspT107I GGYRCC 1 cut(s) 77
BsrBI CCGCTC 1 cut(s) 101
BsrI ACTGG 1 cut(s) 136
Bst2UI CCWGG 1 cut(s) 172
Bst4CI ACNGT 1 cut(s) 224
Bst6I CTCTTC 1 cut(s) 40
BstAPI GCANNNNNTGC 1 cut(s) 197
BstF5I GGATG 1 cut(s) 256
BstFNI CGCG 1 cut(s) 26
BstHHI GCGC 1 cut(s) 28
BstMWI GCNNNNNNNGC 3 cut(s) 197, 293, 302
BstNI CCWGG 1 cut(s) 172
BstSCI CCNGG 1 cut(s) 170
BstSFI CTRYAG 1 cut(s) 336
BstSLI GKGCMC 1 cut(s) 193
BstUI CGCG 1 cut(s) 26
BstV1I GCAGC 1 cut(s) 289
BsuRI GGCC 1 cut(s) 126
BtgZI GCGATG 1 cut(s) 7
BtsCI GGATG 1 cut(s) 256
BtsIMutI CAGTG 1 cut(s) 220
CfoI GCGC 1 cut(s) 28
Csp6I GTAC 2 cut(s) 16, 177
CviJI RGCY 7 cut(s) 74, 126, 146, 281, 296, 318, 341
CviKI_1 RGCY 7 cut(s) 74, 126, 146, 281, 296, 318, 341
CviQI GTAC 2 cut(s) 16, 177
DrdI GACNNNNNNGTC 1 cut(s) 17
DseDI GACNNNNNNGTC 1 cut(s) 17
Eam1104I CTCTTC 1 cut(s) 40
EarI CTCTTC 1 cut(s) 40
Eco32I GATATC 1 cut(s) 239
EcoRII CCWGG 1 cut(s) 170
EcoRV GATATC 1 cut(s) 239
FaiI YATR 4 cut(s) 94, 117, 284, 378
FblI GTMKAC 1 cut(s) 9
Fnu4HI GCNGC 2 cut(s) 303, 369
FokI GGATG 1 cut(s) 263
Fsp4HI GCNGC 2 cut(s) 303, 369
GlaI GCGC 1 cut(s) 27
GluI GCNGC 2 cut(s) 303, 369
GsaI CCCAGC 2 cut(s) 150, 279
HaeIII GGCC 1 cut(s) 126
HhaI GCGC 1 cut(s) 28
Hin6I GCGC 1 cut(s) 26
HinP1I GCGC 1 cut(s) 26
HincII GTYRAC 1 cut(s) 61
HindII GTYRAC 1 cut(s) 61
HinfI GANTC 1 cut(s) 331
HphI GGTGA 2 cut(s) 271, 340
Hpy166II GTNNAC 5 cut(s) 10, 61, 177, 191, 220
Hpy188I TCNGA 1 cut(s) 68
Hpy188III TCNNGA 2 cut(s) 312, 402
Hpy8I GTNNAC 5 cut(s) 10, 61, 177, 191, 220
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 1 cut(s) 241
HpyCH4III ACNGT 1 cut(s) 224
HpyCH4IV ACGT 2 cut(s) 18, 63
HpyCH4V TGCA 2 cut(s) 191, 232
HpyF10VI GCNNNNNNNGC 3 cut(s) 197, 293, 302
HpySE526I ACGT 2 cut(s) 18, 63
HspAI GCGC 1 cut(s) 26
LguI GCTCTTC 1 cut(s) 40
LmnI GCTCC 3 cut(s) 101, 106, 293
Lsp1109I GCAGC 1 cut(s) 289
LweI GCATC 1 cut(s) 100
MaeII ACGT 2 cut(s) 18, 63
MbiI CCGCTC 1 cut(s) 101
MboII GAAGA 2 cut(s) 27, 42
MhlI GDGCHC 2 cut(s) 35, 193
MluCI AATT 3 cut(s) 119, 204, 363
MmeI TCCRAC 1 cut(s) 46
MspR9I CCNGG 1 cut(s) 172
MvaI CCWGG 1 cut(s) 172
MvnI CGCG 1 cut(s) 26
MwoI GCNNNNNNNGC 3 cut(s) 197, 293, 302
NlaIV GGNNCC 1 cut(s) 79
PciSI GCTCTTC 1 cut(s) 40
PfeI GAWTC 1 cut(s) 331
Pfl23II CGTACG 1 cut(s) 15
PkrI GCNGC 2 cut(s) 304, 370
Psp6I CCWGG 1 cut(s) 170
PspFI CCCAGC 2 cut(s) 146, 275
PspGI CCWGG 1 cut(s) 170
PspLI CGTACG 1 cut(s) 15
PspN4I GGNNCC 1 cut(s) 79
RsaI GTAC 2 cut(s) 17, 178
RsaNI GTAC 2 cut(s) 16, 177
SapI GCTCTTC 1 cut(s) 40
SatI GCNGC 2 cut(s) 303, 369
ScrFI CCNGG 1 cut(s) 172
SduI GDGCHC 2 cut(s) 35, 193
SfaNI GCATC 1 cut(s) 100
SfcI CTRYAG 1 cut(s) 336
Sse9I AATT 3 cut(s) 119, 204, 363
SsiI CCGC 2 cut(s) 99, 369
StyD4I CCNGG 1 cut(s) 170
TaaI ACNGT 1 cut(s) 224
TaiI ACGT 2 cut(s) 21, 66
TasI AATT 3 cut(s) 119, 204, 363
TauI GCSGC 1 cut(s) 371
TfiI GAWTC 1 cut(s) 331
TscAI CASTG 1 cut(s) 227
TseI GCWGC 1 cut(s) 302
TspRI CASTG 1 cut(s) 227
VneI GTGCAC 1 cut(s) 189
XapI RAATTY 2 cut(s) 119, 204
XmiI GTMKAC 1 cut(s) 9
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.