RchiOBHm_Chr6g0302491

39S ribosomal protein L53/MRP-L53

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002943
Physical Location & Seq
Forward (+)
62587962 .. 62589805
1844 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 387 bp
ATGTTGAAGTTCCTCTCCCGAGTGAGGATCGAGTTCAACGCGTTGGACCCACGCACAGCTTCGTGCTTGGAGTTCTTGGCGCAGTGCAACGCCACCAAGGCCAAGGAGTCCAATCCCGGCTGCGCCGTCCAAGTCAAGCGCCGCACCGACGACGAGCCCCCGAAGATCACCGTCACCTTCGTCAACGGAGTCGAGGAGGTCTTCGACGCCACTGCCACGCCCGCCCAGAGCATAAGGACCATGATTCTCGAAAAGGGTCAGATGCTCGAGACCGAGCAGATGTTCCGCGACGCCGGCGAGGCCTGGCCGGTTCTTATTCCGGCCGAGGAGCTCACCCAACCCTCGCCTGGTACCAAGCCAAGGAAAGCAGAAGAGAAGAAGCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MRP_L53

Protein Analysis

128

Amino Acids

14.29

Weight (kDa)

5.61

Isoelectric Point (pI)

51.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MRP_L53 PF10780 12 - 63 1.3e-15 39S ribosomal protein L53/MRP-L53
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 350
AccB1I GGYRCC 1 cut(s) 350
AccII CGCG 2 cut(s) 41, 288
AciI CCGC 3 cut(s) 142, 222, 286
AclWI GGATC 1 cut(s) 35
AcoI YGGCCR 2 cut(s) 305, 321
AcyI GRCGYC 2 cut(s) 207, 291
AfaI GTAC 1 cut(s) 352
AfiI CCNNNNNNNGG 3 cut(s) 24, 347, 360
AflIII ACRYGT 1 cut(s) 39
AgsI TTSAA 2 cut(s) 7, 37
AjnI CCWGG 2 cut(s) 302, 346
AluBI AGCT 2 cut(s) 59, 331
AluI AGCT 2 cut(s) 59, 331
Alw21I GWGCWC 1 cut(s) 333
Alw26I GTCTC 1 cut(s) 263
AlwI GGATC 1 cut(s) 35
Ama87I CYCGRG 2 cut(s) 18, 266
AoxI GGCC 4 cut(s) 99, 300, 305, 321
ApeKI GCWGC 1 cut(s) 120
Asp718I GGTACC 1 cut(s) 350
AspLEI GCGC 3 cut(s) 82, 125, 141
AspS9I GGNCC 2 cut(s) 46, 237
AsuC2I CCSGG 1 cut(s) 117
AsuHPI GGTGA 3 cut(s) 160, 166, 325
AvaI CYCGRG 2 cut(s) 18, 266
AvaII GGWCC 2 cut(s) 46, 237
BanI GGYRCC 1 cut(s) 350
BanII GRGCYC 2 cut(s) 159, 333
BbsI GAAGAC 1 cut(s) 193
Bbv12I GWGCWC 1 cut(s) 333
BbvI GCAGC 1 cut(s) 107
BceAI ACGGC 1 cut(s) 110
BcgI CGANNNNNNTGC 2 cut(s) 194, 228
BciT130I CCWGG 2 cut(s) 304, 348
BcnI CCSGG 1 cut(s) 117
BcoDI GTCTC 1 cut(s) 263
BfoI RGCGCY 1 cut(s) 142
BglI GCCNNNNNGGC 2 cut(s) 98, 299
BisI GCNGC 2 cut(s) 121, 142
BlsI GCNGC 2 cut(s) 122, 143
Bme1390I CCNGG 3 cut(s) 117, 304, 348
Bme18I GGWCC 2 cut(s) 46, 237
BmeT110I CYCGRG 2 cut(s) 18, 266
BmgT120I GGNCC 2 cut(s) 46, 237
BmiI GGNNCC 2 cut(s) 48, 352
BmrFI CCNGG 3 cut(s) 117, 304, 348
BmsI GCATC 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 193
BpuMI CCSGG 1 cut(s) 117
BsaHI GRCGYC 2 cut(s) 207, 291
BsaI GGTCTC 1 cut(s) 263
BsaJI CCNNGG 4 cut(s) 96, 102, 324, 359
Bsc4I CCNNNNNNNGG 3 cut(s) 24, 347, 360
Bse118I RCCGGY 2 cut(s) 293, 307
BseBI CCWGG 2 cut(s) 304, 348
BseDI CCNNGG 4 cut(s) 96, 102, 324, 359
BseLI CCNNNNNNNGG 3 cut(s) 24, 347, 360
BseRI GAGGAG 2 cut(s) 209, 341
BseX3I CGGCCG 1 cut(s) 321
BseXI GCAGC 1 cut(s) 107
Bsh1236I CGCG 2 cut(s) 41, 288
Bsh1285I CGRYCG 1 cut(s) 324
BshFI GGCC 4 cut(s) 101, 302, 307, 323
BshNI GGYRCC 1 cut(s) 350
BsiEI CGRYCG 1 cut(s) 324
BsiHKAI GWGCWC 1 cut(s) 333
BsiHKCI CYCGRG 2 cut(s) 18, 266
BsiSI CCGG 4 cut(s) 117, 294, 308, 320
BslI CCNNNNNNNGG 3 cut(s) 24, 347, 360
BsmAI GTCTC 1 cut(s) 263
BsnI GGCC 4 cut(s) 101, 302, 307, 323
Bso31I GGTCTC 1 cut(s) 263
BsoBI CYCGRG 2 cut(s) 18, 266
Bsp1286I GDGCHC 2 cut(s) 159, 333
Bsp143I GATC 2 cut(s) 27, 165
BspACI CCGC 3 cut(s) 142, 222, 286
BspANI GGCC 4 cut(s) 101, 302, 307, 323
BspFNI CGCG 2 cut(s) 41, 288
BspLI GGNNCC 2 cut(s) 48, 352
BspPI GGATC 1 cut(s) 35
BspT107I GGYRCC 1 cut(s) 350
BspTNI GGTCTC 1 cut(s) 263
BsrFI RCCGGY 2 cut(s) 293, 307
BssAI RCCGGY 2 cut(s) 293, 307
BssECI CCNNGG 4 cut(s) 96, 102, 324, 359
BssMI GATC 2 cut(s) 27, 165
BssNI GRCGYC 2 cut(s) 207, 291
BssT1I CCWWGG 3 cut(s) 96, 102, 359
Bst2UI CCWGG 2 cut(s) 304, 348
Bst4CI ACNGT 1 cut(s) 172
Bst6I CTCTTC 1 cut(s) 366
BstACI GRCGYC 2 cut(s) 207, 291
BstC8I GCNNGC 2 cut(s) 222, 295
BstFNI CGCG 2 cut(s) 41, 288
BstH2I RGCGCY 1 cut(s) 142
BstHHI GCGC 3 cut(s) 82, 125, 141
BstKTI GATC 2 cut(s) 30, 168
BstMAI GTCTC 1 cut(s) 263
BstMBI GATC 2 cut(s) 27, 165
BstMCI CGRYCG 1 cut(s) 324
BstMWI GCNNNNNNNGC 4 cut(s) 98, 221, 294, 299
BstNI CCWGG 2 cut(s) 304, 348
BstSCI CCNGG 3 cut(s) 115, 302, 346
BstUI CGCG 2 cut(s) 41, 288
BstV1I GCAGC 1 cut(s) 107
BstV2I GAAGAC 1 cut(s) 193
BstZI CGGCCG 1 cut(s) 321
BsuRI GGCC 4 cut(s) 101, 302, 307, 323
BtsI GCAGTG 2 cut(s) 89, 210
BtsIMutI CAGTG 2 cut(s) 89, 210
Cac8I GCNNGC 2 cut(s) 222, 295
CfoI GCGC 3 cut(s) 82, 125, 141
Cfr10I RCCGGY 2 cut(s) 293, 307
Cfr13I GGNCC 2 cut(s) 46, 237
CseI GACGC 2 cut(s) 215, 299
Csp6I GTAC 1 cut(s) 351
CviAII CATG 1 cut(s) 241
CviJI RGCY 9 cut(s) 59, 101, 120, 157, 302, 307, 323, 331, 358
CviKI_1 RGCY 9 cut(s) 59, 101, 120, 157, 302, 307, 323, 331, 358
CviQI GTAC 1 cut(s) 351
DpnI GATC 2 cut(s) 29, 167
DpnII GATC 2 cut(s) 27, 165
EaeI YGGCCR 2 cut(s) 305, 321
EagI CGGCCG 1 cut(s) 321
Eam1104I CTCTTC 1 cut(s) 366
EarI CTCTTC 1 cut(s) 366
Ecl136II GAGCTC 1 cut(s) 331
EclXI CGGCCG 1 cut(s) 321
Eco130I CCWWGG 3 cut(s) 96, 102, 359
Eco147I AGGCCT 1 cut(s) 302
Eco24I GRGCYC 2 cut(s) 159, 333
Eco31I GGTCTC 1 cut(s) 263
Eco47I GGWCC 2 cut(s) 46, 237
Eco52I CGGCCG 1 cut(s) 321
Eco53kI GAGCTC 1 cut(s) 331
Eco88I CYCGRG 2 cut(s) 18, 266
EcoICRI GAGCTC 1 cut(s) 331
EcoRII CCWGG 2 cut(s) 302, 346
EcoT14I CCWWGG 3 cut(s) 96, 102, 359
EcoT38I GRGCYC 2 cut(s) 159, 333
ErhI CCWWGG 3 cut(s) 96, 102, 359
FaeI CATG 1 cut(s) 244
FaiI YATR 2 cut(s) 233, 242
FatI CATG 1 cut(s) 240
FauI CCCGC 1 cut(s) 229
Fnu4HI GCNGC 2 cut(s) 121, 142
FriOI GRGCYC 2 cut(s) 159, 333
Fsp4HI GCNGC 2 cut(s) 121, 142
GlaI GCGC 3 cut(s) 81, 124, 140
GluI GCNGC 2 cut(s) 121, 142
HaeII RGCGCY 1 cut(s) 142
HaeIII GGCC 4 cut(s) 101, 302, 307, 323
HapII CCGG 4 cut(s) 117, 294, 308, 320
HgaI GACGC 2 cut(s) 215, 299
HhaI GCGC 3 cut(s) 82, 125, 141
Hin1I GRCGYC 2 cut(s) 207, 291
Hin1II CATG 1 cut(s) 244
Hin6I GCGC 3 cut(s) 80, 123, 139
HinP1I GCGC 3 cut(s) 80, 123, 139
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 3 cut(s) 107, 189, 244
HpaII CCGG 4 cut(s) 117, 294, 308, 320
HphI GGTGA 3 cut(s) 160, 166, 325
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 1 cut(s) 261
Hpy188III TCNNGA 3 cut(s) 18, 248, 268
Hpy8I GTNNAC 1 cut(s) 184
Hpy99I CGWCG 4 cut(s) 152, 155, 209, 293
HpyAV CCTTC 1 cut(s) 187
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4V TGCA 1 cut(s) 87
HpyF10VI GCNNNNNNNGC 4 cut(s) 98, 221, 294, 299
Hsp92I GRCGYC 2 cut(s) 207, 291
Hsp92II CATG 1 cut(s) 244
HspAI GCGC 3 cut(s) 80, 123, 139
KpnI GGTACC 1 cut(s) 354
KroI GCCGGC 1 cut(s) 293
KroNI GCCGGC 1 cut(s) 295
Kzo9I GATC 2 cut(s) 27, 165
LmnI GCTCC 1 cut(s) 328
LpnPI CCDG 9 cut(s) 130, 239, 289, 307, 316, 321, 333, 333, 360
Lsp1109I GCAGC 1 cut(s) 107
LweI GCATC 1 cut(s) 252
MaeIII GTNAC 1 cut(s) 172
MalI GATC 2 cut(s) 29, 167
MboI GATC 2 cut(s) 27, 165
MboII GAAGA 3 cut(s) 175, 193, 383
MhlI GDGCHC 2 cut(s) 159, 333
MluI ACGCGT 1 cut(s) 39
MlyI GAGTC 2 cut(s) 116, 198
MmeI TCCRAC 1 cut(s) 24
MnlI CCTC 7 cut(s) 18, 23, 187, 190, 292, 319, 352
MreI CGCCGGCG 1 cut(s) 293
MroNI GCCGGC 1 cut(s) 293
MspI CCGG 4 cut(s) 117, 294, 308, 320
MspR9I CCNGG 3 cut(s) 117, 304, 348
MvaI CCWGG 2 cut(s) 304, 348
MvnI CGCG 2 cut(s) 41, 288
MwoI GCNNNNNNNGC 4 cut(s) 98, 221, 294, 299
NaeI GCCGGC 1 cut(s) 295
NciI CCSGG 1 cut(s) 117
NdeII GATC 2 cut(s) 27, 165
NgoMIV GCCGGC 1 cut(s) 293
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 2 cut(s) 48, 352
NmeAIII GCCGAG 1 cut(s) 349
NmuCI GTSAC 1 cut(s) 172
PaeR7I CTCGAG 1 cut(s) 266
PceI AGGCCT 1 cut(s) 302
PdiI GCCGGC 1 cut(s) 295
PfeI GAWTC 1 cut(s) 244
PkrI GCNGC 2 cut(s) 122, 143
PleI GAGTC 2 cut(s) 115, 197
PpsI GAGTC 2 cut(s) 115, 197
Psp124BI GAGCTC 1 cut(s) 333
Psp6I CCWGG 2 cut(s) 302, 346
PspGI CCWGG 2 cut(s) 302, 346
PspN4I GGNNCC 2 cut(s) 48, 352
PspPI GGNCC 2 cut(s) 46, 237
RsaI GTAC 1 cut(s) 352
RsaNI GTAC 1 cut(s) 351
SacI GAGCTC 1 cut(s) 333
SatI GCNGC 2 cut(s) 121, 142
Sau3AI GATC 2 cut(s) 27, 165
Sau96I GGNCC 2 cut(s) 46, 237
SchI GAGTC 2 cut(s) 116, 198
ScrFI CCNGG 3 cut(s) 117, 304, 348
SduI GDGCHC 2 cut(s) 159, 333
SetI ASST 4 cut(s) 61, 179, 201, 333
SfaNI GCATC 1 cut(s) 252
Sfr274I CTCGAG 1 cut(s) 266
SgrAI CRCCGGYG 1 cut(s) 293
SinI GGWCC 2 cut(s) 46, 237
SlaI CTCGAG 1 cut(s) 266
SmlI CTYRAG 1 cut(s) 266
SmoI CTYRAG 1 cut(s) 266
SseBI AGGCCT 1 cut(s) 302
SsiI CCGC 3 cut(s) 142, 222, 286
SstI GAGCTC 1 cut(s) 333
StuI AGGCCT 1 cut(s) 302
StyD4I CCNGG 3 cut(s) 115, 302, 346
StyI CCWWGG 3 cut(s) 96, 102, 359
TaaI ACNGT 1 cut(s) 172
TaqI TCGA 5 cut(s) 30, 192, 204, 249, 267
TaqII GACCGA 1 cut(s) 287
TauI GCSGC 1 cut(s) 144
TfiI GAWTC 1 cut(s) 244
TscAI CASTG 2 cut(s) 89, 217
TseFI GTSAC 1 cut(s) 172
TseI GCWGC 1 cut(s) 120
Tsp45I GTSAC 1 cut(s) 172
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 2 cut(s) 89, 217
VpaK11BI GGWCC 2 cut(s) 46, 237
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XhoI CTCGAG 1 cut(s) 266
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.