RchiOBHm_Chr5g0000121

von Willebrand factor (vWF) type A domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010533
Physical Location & Seq
Reverse (-)
126393 .. 129218
2826 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1683 bp
ATGGGAGGAGGAGGAGGACTGCGAAAAGCGGCCAAGAAGATGGTGGTGGCAGCTGCCAATGCATGTGGTGGTTCCTTCTCTTCCAGGAGGAAGCCTCTGGTGGACCCCATCGTCTTCTTCGATCATACCACCGCCACTGCCTCTATCTCAGGTTCTTCGGCTGTGTCTCCCACCAACTCCACAGCAGCAGAAGAAGAAGAAGAAGAAGAAGAAGAATGCATTGCCAAGGAGATGGAATCTGCAATCACCAATTCTAACAATGTACCTTCCTCTTCTTCTTCTAATAAGAACTTGTGCGCAATATGTCTAGACCCTCTGAGTTACCATAGCAAGGGACGGATCTCAGGGCAGGCTATATTCACGGCCCAGTGCTCCCATGCATTCCACTTTGCCTGCATCTCTTCCAATGTCCGCCATGGTAGTGTCACCTGCCCCATCTGCCGTGCCCATTGGACTCAGCTGCCCCGCAATCTGAACCCACCTGGTGGTTCCCTTTCCTCTTGCAACCCAACTGATCCCATCCTCCAAATCCTTGATGACTCTATAGCCACTTTCCGCATCCACAGGCGCTCTTTCCTGCGCTCTGCTCGCTATGATGATGATGACCCAATTGAGCCTCCCGATCACATGCCTAATTTCCCCCGTCTCCAATTCTCTCTAGCACCCTTACCACCTAGTACTCCACCCCATAGCTTTCAACCGTCCTGGTGCACCACACACCCATATCATCCACCCCCACATCACCTGAGCTATAGCTCCACATCATTGTTACAATCGCCAACCAGGCCAAAGCCCTATACTACTATGTATGCCTCTTCGGACAGAGCTTATCTTTCGGTGAAATTGGCAGAGCAGCGAGCAACTGATTTTGTCTTGGTTGCTAGTCCCAACGGACCACACTTGAGGCTTCTCAAGCAGTGCATGGCATTGGTGGTTTTCTCCCTTAGACCTATTGATCGTTTGGCCATTGTTACCTACTCATCAGCTGCTGCTCGTGTCTTCCCTCTAAGACGCATGACATCTTATGGGAAGCGAACAGCCCAGCAGGTTATTGACCGCCTGTTCTACATGGGGCAAGCAGATCCAGTGGAAGGGCTCAAGAAAGGGATCAAGATACTTCAAGATCGTGTATACAAGAATCAAAATTCGGGTATCCTGCATCTTTCTGACACCCCAACTCGATCCTACCATGCTGCTCTCAATATGGACGTGCCCATTCCAGTGCATCGTTTTCATGTAGGATTTGGATTTGGCACTTCAAATGGTTTTATCATGCATGAATTTGAAGAGTTCCTCAGAAGGCTCATAGGTGGTGTTGTTAGAGAAATCCAGTTAAGGATCAGCACAACGGAGGAAGCCAGCAGTAGGATTGTAAGAATAGGAGAGCTAAGAAACGGAGAAGAAAGGAAAATCTTAGTGGACTTGAGTGTTTGTGGACATATTTGTGTGGGATACAGCTACATAGAGGATGGTGAGATTGACGAACCTATCACAACAGGGGAAACTATGGTGAGCATAGGAGACGGTAAAAGTAACATGAGTGCAACTGAAGCAGCTTCTGCTGGGTCAGGGACGAGCGGTCCAATTATTGGTGGGAGGCCTAGTAGTGCAGACAGTTGGGACTACCATGATCCTTACATGGCTAGAAGATGGGCCAAACATTTGCATGGCTACAGGCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
VWA_2 zf-C3HC4 zf-RING_2 zf-rbx1

Protein Analysis

560

Amino Acids

61.44

Weight (kDa)

8.18

Isoelectric Point (pI)

60.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 98 - 148 2.1e-07 Ring finger domain
zf-rbx1 PF12678 98 - 148 1.3e-06 RING-H2 zinc finger domain
zf-C3HC4 PF00097 99 - 147 1.9e-07 Zinc finger, C3HC4 type (RING finger)
zf-RING_11 PF17123 99 - 133 1.8e-06 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 437
AasI GACNNNNNNGTC 1 cut(s) 110
Acc16I TGCGCA 1 cut(s) 298
Acc36I ACCTGC 2 cut(s) 437, 1036
AccB7I CCANNNNNTGG 2 cut(s) 485, 1589
AccBSI CCGCTC 1 cut(s) 1578
AccI GTMKAC 1 cut(s) 1131
AciI CCGC 7 cut(s) 29, 132, 412, 466, 556, 1057, 1578
AclWI GGATC 7 cut(s) 347, 509, 1076, 1115, 1176, 1346, 1625
AcoI YGGCCR 2 cut(s) 30, 963
AcsI RAATTY 2 cut(s) 1144, 1280
AcuI CTGAAG 1 cut(s) 1569
AdeI CACNNNGTG 1 cut(s) 485
AfaI GTAC 2 cut(s) 264, 679
AfiI CCNNNNNNNGG 5 cut(s) 331, 485, 1091, 1365, 1589
AgsI TTSAA 4 cut(s) 698, 1121, 1260, 1286
AhdI GACNNNNNGTC 1 cut(s) 1578
AjiI CACGTC 1 cut(s) 1210
AjnI CCWGG 4 cut(s) 83, 481, 704, 782
Alw21I GWGCWC 2 cut(s) 374, 713
Alw26I GTCTC 3 cut(s) 171, 650, 1515
Alw44I GTGCAC 1 cut(s) 709
AlwI GGATC 7 cut(s) 347, 509, 1076, 1115, 1176, 1346, 1625
AlwNI CAGNNNCTG 2 cut(s) 989, 1559
AoxI GGCC 6 cut(s) 30, 363, 785, 963, 1598, 1653
ApaLI GTGCAC 1 cut(s) 709
ApeKI GCWGC 9 cut(s) 50, 53, 185, 460, 853, 986, 989, 1193, 1553
ApoI RAATTY 2 cut(s) 1144, 1280
AspLEI GCGC 3 cut(s) 299, 570, 582
AspS9I GGNCC 5 cut(s) 103, 364, 893, 1580, 1653
AsuHPI GGTGA 6 cut(s) 238, 418, 734, 850, 1484, 1522
AvaII GGWCC 3 cut(s) 103, 893, 1580
BaeGI GKGCMC 3 cut(s) 448, 713, 1215
BalI TGGCCA 1 cut(s) 965
BanII GRGCYC 1 cut(s) 1098
BauI CACGAG 1 cut(s) 993
BbsI GAAGAC 2 cut(s) 106, 991
Bbv12I GWGCWC 2 cut(s) 374, 713
BbvI GCAGC 9 cut(s) 40, 62, 197, 447, 865, 973, 976, 1180, 1565
BccI CCATC 7 cut(s) 34, 116, 226, 443, 527, 1463, 1644
BceAI ACGGC 2 cut(s) 378, 426
BciT130I CCWGG 4 cut(s) 85, 483, 706, 784
BciVI GTATCC 2 cut(s) 1163, 1445
BcoDI GTCTC 3 cut(s) 171, 650, 1515
BfaI CTAG 6 cut(s) 308, 659, 675, 882, 1602, 1644
BfmI CTRYAG 3 cut(s) 543, 751, 1672
BfoI RGCGCY 1 cut(s) 571
BfuAI ACCTGC 2 cut(s) 437, 1036
BfuI GTATCC 2 cut(s) 1163, 1445
BglI GCCNNNNNGGC 1 cut(s) 784
BmcAI AGTACT 1 cut(s) 679
Bme1390I CCNGG 4 cut(s) 85, 483, 706, 784
Bme18I GGWCC 3 cut(s) 103, 893, 1580
BmeRI GACNNNNNGTC 1 cut(s) 1578
BmgBI CACGTC 1 cut(s) 1210
BmgT120I GGNCC 5 cut(s) 103, 364, 893, 1580, 1653
BmiI GGNNCC 3 cut(s) 73, 105, 490
BmrFI CCNGG 4 cut(s) 85, 483, 706, 784
BmrI ACTGGG 1 cut(s) 361
BmsI GCATC 4 cut(s) 405, 567, 1168, 1234
BmuI ACTGGG 1 cut(s) 361
BpiI GAAGAC 2 cut(s) 106, 991
BplI GAGNNNNNCTC 2 cut(s) 79, 111
Bpu10I CCTNAGC 1 cut(s) 746
BpuEI CTTGAG 4 cut(s) 896, 922, 1082, 1444
BsaJI CCNNGG 2 cut(s) 225, 415
BsaXI ACNNNNNCTCC 2 cut(s) 1588, 1618
Bsc4I CCNNNNNNNGG 5 cut(s) 331, 485, 1091, 1365, 1589
Bse1I ACTGG 4 cut(s) 367, 1085, 1220, 1330
Bse3DI GCAATG 1 cut(s) 219
BseBI CCWGG 4 cut(s) 85, 483, 706, 784
BseDI CCNNGG 2 cut(s) 225, 415
BseGI GGATG 4 cut(s) 519, 558, 727, 1474
BseLI CCNNNNNNNGG 5 cut(s) 331, 485, 1091, 1365, 1589
BseMI GCAATG 1 cut(s) 219
BseMII CTCAG 6 cut(s) 162, 308, 357, 470, 737, 1309
BseNI ACTGG 4 cut(s) 367, 1085, 1220, 1330
BseRI GAGGAG 3 cut(s) 21, 24, 27
BseSI GKGCMC 3 cut(s) 448, 713, 1215
BseXI GCAGC 9 cut(s) 40, 62, 197, 447, 865, 973, 976, 1180, 1565
BseYI CCCAGC 2 cut(s) 1041, 1562
BsgI GTGCAG 1 cut(s) 1629
BshFI GGCC 6 cut(s) 32, 365, 787, 965, 1600, 1655
BsiHKAI GWGCWC 2 cut(s) 374, 713
BslFI GGGAC 4 cut(s) 348, 870, 1585, 1634
BslI CCNNNNNNNGG 5 cut(s) 331, 485, 1091, 1365, 1589
BsmAI GTCTC 3 cut(s) 171, 650, 1515
BsmBI CGTCTC 2 cut(s) 650, 1515
BsmFI GGGAC 4 cut(s) 348, 870, 1585, 1634
BsmI GAATGC 2 cut(s) 221, 380
BsnI GGCC 6 cut(s) 32, 365, 787, 965, 1600, 1655
Bsp1286I GDGCHC 5 cut(s) 374, 448, 713, 1098, 1215
Bsp19I CCATGG 1 cut(s) 415
BspACI CCGC 7 cut(s) 29, 132, 412, 466, 556, 1057, 1578
BspANI GGCC 6 cut(s) 32, 365, 787, 965, 1600, 1655
BspCNI CTCAG 6 cut(s) 161, 309, 356, 469, 738, 1308
BspLI GGNNCC 3 cut(s) 73, 105, 490
BspMI ACCTGC 2 cut(s) 437, 1036
BspPI GGATC 7 cut(s) 347, 509, 1076, 1115, 1176, 1346, 1625
BsrBI CCGCTC 1 cut(s) 1578
BsrDI GCAATG 1 cut(s) 219
BsrI ACTGG 4 cut(s) 367, 1085, 1220, 1330
BssECI CCNNGG 2 cut(s) 225, 415
BssNAI GTATAC 1 cut(s) 1132
BssSI CACGAG 1 cut(s) 993
BssT1I CCWWGG 2 cut(s) 225, 415
Bst1107I GTATAC 1 cut(s) 1132
Bst2BI CACGAG 1 cut(s) 993
Bst2UI CCWGG 4 cut(s) 85, 483, 706, 784
Bst4CI ACNGT 3 cut(s) 702, 1526, 1616
Bst6I CTCTTC 5 cut(s) 85, 277, 406, 820, 1281
BstAPI GCANNNNNTGC 1 cut(s) 1559
BstC8I GCNNGC 6 cut(s) 351, 394, 589, 858, 1077, 1360
BstDSI CCRYGG 1 cut(s) 415
BstF5I GGATG 4 cut(s) 519, 558, 727, 1474
BstH2I RGCGCY 1 cut(s) 571
BstHHI GCGC 3 cut(s) 299, 570, 582
BstMAI GTCTC 3 cut(s) 171, 650, 1515
BstMWI GCNNNNNNNGC 7 cut(s) 59, 438, 588, 784, 913, 1550, 1559
BstNI CCWGG 4 cut(s) 85, 483, 706, 784
BstNSI RCATGY 2 cut(s) 66, 631
BstSCI CCNGG 4 cut(s) 83, 481, 704, 782
BstSFI CTRYAG 3 cut(s) 543, 751, 1672
BstSLI GKGCMC 3 cut(s) 448, 713, 1215
BstV1I GCAGC 9 cut(s) 40, 62, 197, 447, 865, 973, 976, 1180, 1565
BstV2I GAAGAC 2 cut(s) 106, 991
BstX2I RGATCY 2 cut(s) 339, 1081
BstXI CCANNNNNNTGG 2 cut(s) 40, 232
BstYI RGATCY 2 cut(s) 339, 1081
BstZ17I GTATAC 1 cut(s) 1132
BsuI GTATCC 2 cut(s) 1163, 1445
BsuRI GGCC 6 cut(s) 32, 365, 787, 965, 1600, 1655
BtgI CCRYGG 1 cut(s) 415
BtrI CACGTC 1 cut(s) 1210
BtsCI GGATG 4 cut(s) 519, 558, 727, 1474
BtsI GCAGTG 2 cut(s) 135, 923
BtsIMutI CAGTG 5 cut(s) 135, 374, 923, 1092, 1227
BveI ACCTGC 2 cut(s) 437, 1036
Cac8I GCNNGC 6 cut(s) 351, 394, 589, 858, 1077, 1360
CaiI CAGNNNCTG 2 cut(s) 989, 1559
CfoI GCGC 3 cut(s) 299, 570, 582
Cfr13I GGNCC 5 cut(s) 103, 364, 893, 1580, 1653
CseI GACGC 1 cut(s) 1020
CsiI ACCWGGT 1 cut(s) 481
Csp6I GTAC 2 cut(s) 263, 678
CspCI CAANNNNNGTGG 2 cut(s) 46, 81
CviQI GTAC 2 cut(s) 263, 678
DraIII CACNNNGTG 1 cut(s) 485
DrdI GACNNNNNNGTC 1 cut(s) 110
DriI GACNNNNNGTC 1 cut(s) 1578
DseDI GACNNNNNNGTC 1 cut(s) 110
EaeI YGGCCR 2 cut(s) 30, 963
Eam1104I CTCTTC 5 cut(s) 85, 277, 406, 820, 1281
Eam1105I GACNNNNNGTC 1 cut(s) 1578
EarI CTCTTC 5 cut(s) 85, 277, 406, 820, 1281
EciI GGCGGA 1 cut(s) 401
Eco130I CCWWGG 2 cut(s) 225, 415
Eco147I AGGCCT 1 cut(s) 1600
Eco24I GRGCYC 1 cut(s) 1098
Eco47I GGWCC 3 cut(s) 103, 893, 1580
Eco57I CTGAAG 1 cut(s) 1569
EcoRII CCWGG 4 cut(s) 83, 481, 704, 782
EcoT14I CCWWGG 2 cut(s) 225, 415
EcoT22I ATGCAT 4 cut(s) 64, 221, 382, 1278
EcoT38I GRGCYC 1 cut(s) 1098
ErhI CCWWGG 2 cut(s) 225, 415
Esp3I CGTCTC 2 cut(s) 650, 1515
FaqI GGGAC 4 cut(s) 348, 870, 1585, 1634
FauI CCCGC 1 cut(s) 473
FblI GTMKAC 1 cut(s) 1131
FokI GGATG 4 cut(s) 506, 545, 714, 1481
FriOI GRGCYC 1 cut(s) 1098
FspBI CTAG 6 cut(s) 308, 659, 675, 882, 1602, 1644
FspI TGCGCA 1 cut(s) 298
GlaI GCGC 3 cut(s) 298, 569, 581
GsaI CCCAGC 2 cut(s) 1045, 1566
HaeII RGCGCY 1 cut(s) 571
HaeIII GGCC 6 cut(s) 32, 365, 787, 965, 1600, 1655
HgaI GACGC 1 cut(s) 1020
HhaI GCGC 3 cut(s) 299, 570, 582
Hin6I GCGC 3 cut(s) 297, 568, 580
HinP1I GCGC 3 cut(s) 297, 568, 580
HinfI GANTC 4 cut(s) 236, 454, 539, 1138
HphI GGTGA 6 cut(s) 238, 418, 734, 850, 1484, 1522
Hpy166II GTNNAC 5 cut(s) 103, 711, 1132, 1420, 1436
Hpy188I TCNGA 5 cut(s) 318, 474, 820, 1168, 1298
Hpy188III TCNNGA 5 cut(s) 308, 620, 1099, 1111, 1121
Hpy8I GTNNAC 5 cut(s) 103, 711, 1132, 1420, 1436
HpyAV CCTTC 4 cut(s) 85, 276, 1085, 1293
HpyCH4III ACNGT 3 cut(s) 702, 1526, 1616
HpyCH4IV ACGT 1 cut(s) 1209
HpyF10VI GCNNNNNNNGC 7 cut(s) 59, 438, 588, 784, 913, 1550, 1559
HpySE526I ACGT 1 cut(s) 1209
HspAI GCGC 3 cut(s) 297, 568, 580
LmnI GCTCC 2 cut(s) 377, 761
Lsp1109I GCAGC 9 cut(s) 40, 62, 197, 447, 865, 973, 976, 1180, 1565
LweI GCATC 4 cut(s) 405, 567, 1168, 1234
MabI ACCWGGT 1 cut(s) 481
MaeI CTAG 6 cut(s) 308, 659, 675, 882, 1602, 1644
MaeII ACGT 1 cut(s) 1209
MaeIII GTNAC 5 cut(s) 320, 424, 768, 970, 1532
MbiI CCGCTC 1 cut(s) 1578
MfeI CAATTG 1 cut(s) 609
MflI RGATCY 2 cut(s) 339, 1081
MhlI GDGCHC 5 cut(s) 374, 448, 713, 1098, 1215
MlsI TGGCCA 1 cut(s) 965
MluCI AATT 8 cut(s) 250, 609, 634, 650, 842, 1144, 1280, 1584
MluNI TGGCCA 1 cut(s) 965
MlyI GAGTC 2 cut(s) 448, 533
Mox20I TGGCCA 1 cut(s) 965
Mph1103I ATGCAT 4 cut(s) 64, 221, 382, 1278
MscI TGGCCA 1 cut(s) 965
MseI TTAA 1 cut(s) 1334
MslI CAYNNNNRTG 4 cut(s) 420, 1220, 1443, 1665
Msp20I TGGCCA 1 cut(s) 965
MspA1I CMGCKG 3 cut(s) 53, 460, 986
MspR9I CCNGG 4 cut(s) 85, 483, 706, 784
MunI CAATTG 1 cut(s) 609
Mva1269I GAATGC 2 cut(s) 221, 380
MvaI CCWGG 4 cut(s) 85, 483, 706, 784
MwoI GCNNNNNNNGC 7 cut(s) 59, 438, 588, 784, 913, 1550, 1559
NcoI CCATGG 1 cut(s) 415
NlaIV GGNNCC 3 cut(s) 73, 105, 490
NmuCI GTSAC 1 cut(s) 424
NsbI TGCGCA 1 cut(s) 298
NsiI ATGCAT 4 cut(s) 64, 221, 382, 1278
NspI RCATGY 2 cut(s) 66, 631
PaqCI CACCTGC 1 cut(s) 437
PceI AGGCCT 1 cut(s) 1600
PcsI WCGNNNNNNNCGW 1 cut(s) 117
PctI GAATGC 2 cut(s) 221, 380
PfeI GAWTC 2 cut(s) 236, 1138
PflMI CCANNNNNTGG 2 cut(s) 485, 1589
PfoI TCCNGGA 1 cut(s) 83
PleI GAGTC 2 cut(s) 448, 533
PpsI GAGTC 2 cut(s) 448, 533
Psp6I CCWGG 4 cut(s) 83, 481, 704, 782
PspFI CCCAGC 2 cut(s) 1041, 1562
PspGI CCWGG 4 cut(s) 83, 481, 704, 782
PspN4I GGNNCC 3 cut(s) 73, 105, 490
PspPI GGNCC 5 cut(s) 103, 364, 893, 1580, 1653
PstNI CAGNNNCTG 2 cut(s) 989, 1559
PsuI RGATCY 2 cut(s) 339, 1081
PvuII CAGCTG 3 cut(s) 53, 460, 986
RsaI GTAC 2 cut(s) 264, 679
RsaNI GTAC 2 cut(s) 263, 678
RseI CAYNNNNRTG 4 cut(s) 420, 1220, 1443, 1665
SaqAI TTAA 1 cut(s) 1334
Sau96I GGNCC 5 cut(s) 103, 364, 893, 1580, 1653
ScaI AGTACT 1 cut(s) 679
SchI GAGTC 2 cut(s) 448, 533
ScrFI CCNGG 4 cut(s) 85, 483, 706, 784
SduI GDGCHC 5 cut(s) 374, 448, 713, 1098, 1215
SexAI ACCWGGT 1 cut(s) 481
SfaNI GCATC 4 cut(s) 405, 567, 1168, 1234
SfcI CTRYAG 3 cut(s) 543, 751, 1672
SinI GGWCC 3 cut(s) 103, 893, 1580
SmiMI CAYNNNNRTG 4 cut(s) 420, 1220, 1443, 1665
SmlI CTYRAG 4 cut(s) 901, 911, 1097, 1423
SmoI CTYRAG 4 cut(s) 901, 911, 1097, 1423
Sse9I AATT 8 cut(s) 250, 609, 634, 650, 842, 1144, 1280, 1584
SseBI AGGCCT 1 cut(s) 1600
SsiI CCGC 7 cut(s) 29, 132, 412, 466, 556, 1057, 1578
SspMI CTAG 6 cut(s) 308, 659, 675, 882, 1602, 1644
StuI AGGCCT 1 cut(s) 1600
StyD4I CCNGG 4 cut(s) 83, 481, 704, 782
StyI CCWWGG 2 cut(s) 225, 415
TaaI ACNGT 3 cut(s) 702, 1526, 1616
TaiI ACGT 1 cut(s) 1212
TaqI TCGA 2 cut(s) 120, 1180
TasI AATT 8 cut(s) 250, 609, 634, 650, 842, 1144, 1280, 1584
TatI WGTACW 1 cut(s) 677
TauI GCSGC 1 cut(s) 32
TfiI GAWTC 2 cut(s) 236, 1138
Tru1I TTAA 1 cut(s) 1334
Tru9I TTAA 1 cut(s) 1334
TscAI CASTG 5 cut(s) 142, 374, 923, 1092, 1227
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 9 cut(s) 50, 53, 185, 460, 853, 986, 989, 1193, 1553
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 1223, 1293
TspGWI ACGGA 4 cut(s) 352, 906, 1364, 1410
TspRI CASTG 5 cut(s) 142, 374, 923, 1092, 1227
Van91I CCANNNNNTGG 2 cut(s) 485, 1589
VneI GTGCAC 1 cut(s) 709
VpaK11BI GGWCC 3 cut(s) 103, 893, 1580
XapI RAATTY 2 cut(s) 1144, 1280
XbaI TCTAGA 1 cut(s) 307
XceI RCATGY 2 cut(s) 66, 631
XcmI CCANNNNNNNNNTGG 1 cut(s) 40
XmiI GTMKAC 1 cut(s) 1131
XspI CTAG 6 cut(s) 308, 659, 675, 882, 1602, 1644
ZrmI AGTACT 1 cut(s) 679
Zsp2I ATGCAT 4 cut(s) 64, 221, 382, 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.