Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0013633

Physical Location & Seq

Genomic Coordinates Forward (+)

7229011 .. 7232483

Gene Structure 3473 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1539 bp

ATGGGTTGCCACAAAATTGAACTGGCTCTTGCTTTTTTCATCTGTGCTTCCCTAGCTTGCTTCTCTTATGGTCTCCCTAGTGAGTACTCCATAGTGGGAGGTGAAACAGTGAACTTCCCAGCTGAGGAGAGAGTGGTGGAGCTCTTCAGGCTGTGGAGGGAGAAGCACAGAAAGGTGTATAACCATGTTGAGGAGCATGAGAGGAGGTTTGAGAATTTCAAGAGGAATCTCAGGTTTGTGTTGGAGAAACATGCACAGAAAAAGGCTGCAGCTATTAAGCATGATACTCAGAGAGTCGGGTTGAACAAGTTTGCTGATTTGAGCAATGAGGAGTTCAGGGCGATTTATATGCCGAAAAAGGTCCGAATGCCGATCAGCAAAAGGGACAGACTGGTGAGGAAAATGCAGCAGCAAAAGAAAGCAGAGCTGCCCAGGGATGCTCCTGCAAGTTTGGATTGGAGGAAGGCGGGAATTGTCACTCCTGTTAAGGACCAAGGCGGTTGTGGAAGTTGTTGGGCATTTTCTTCCACTGGAGCCATCGAAGGAATAAATGCACTGGACACCGGAGACCTCATTAGTCTTTCGGAGCAAGAACTTGTGGATTGTGACACTACCAACGAAGGCTGCAGTGGAGGCTACATGGACTACGCTTTCGAATGGGTTATTAGCAATGGTGGCATTGACACAGAATCTGATTATGCTTACACAGGTGTTGATGGTACCTGCAACGTCACCAAGGAGGAAACCAAAGTTGTCACCATCGATGGCTATACAGATGTCGAAGAGACAGAGACCGGTGTTTTCAATGCTGTGCTTCAGCAACCCATTAGCGTTGGTATGGATGGCTCTGCTTTGGATTTTCAATTATACACCGGAGGAATTTATGACGGTGATTGCTCCGACGATCCAAATGACATTGATCATGCCGTTCTAATAGTAGGATATGGTTCTGAAAACGGTGAAGATTACTGGATTGTAAAGAATTCATGGGGAACTAGTTGGGGGATTGAAGGATACTTTTACCTCAGAAGGAACACCGATTTGCCATATGGGGTTTGTGCAGTCAATGCCATGGCTTCATATCCTACCAAAGAGTCAGTTGCACCCTCTCCTTATCCATCTCCAACTCCTCCACCACCACCTCCTACACCTGTCTCTCCACCACCGCCACCAGTGACACCGCCACCACCACCACCACCCTCTCCTTCACCAAGCCAATGCGGAGACTTCTCCTATTGTCCAGCTGATGAGACATGTTGTTGCCTCTATGAATTCTTTGACTACTGCTTGATTTATGGTTGCTGTGAATATGAGAATGCGGTTTGCTGCACTGGAACTGAATACTGCTGTCCAAGTGACTATCCCATTTGTGATATCGAGGAAGGCCTGTGTCTTAAGAATGGCAGAGACTACTTGGGAGTAGCTGCAAAGAAGAGAAAGATTGCCAAGCACAAGTTCCCATGGACTAAAGTAGAGCAAACCGAGAAGACATACCACCCTCTACAGTGGAAGATGAACCGGTTTGCTGCAATGCGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000323 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0006508 GO:0006807 GO:0008150 GO:0008152 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009987 GO:0016787 GO:0019538 GO:0030163 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044424 GO:0044444 GO:0044464 GO:0051603 GO:0070011 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Granulin Inhibitor_I29 Peptidase_C1

Protein Analysis

512

Amino Acids

57.18

Weight (kDa)

5.04

Isoelectric Point (pI)

49.71

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	49 - 112	2.2e-13	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	148 - 363	4e-79	Papain family cysteine protease
Granulin	PF00396	418 - 465	6.3e-07	Granulin

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013633)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g07700
malus_domestica	MD05G1300100.v1.1
prunus_persica	Prupe.4G062200_v2.0.a1 Prupe.4G062200_v2.0.a1 Prupe.4G062200_v2.0.a1
pyrus_communis	pycom05g27800 pycom05g27830
rosa_chinensis	RchiOBHm_Chr5g0010931
rosa_laevigata	RLG00000031798
rosa_multiflora	Rmu_sc0001895.1_g000004
rosa_roxburghii	Rroxscaffold_1G00065440
rosa_rugosa	Rorug04G0453800
rosa_samantha	Rh5AG086400 Rh5BG080900 Rh5CG093400 Rh5DG081000
rosa_wichuraiana	Rw0G017430 Rw0G017880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	731
Acc65I	GGTACC	1 cut(s)	719
AccB1I	GGYRCC	1 cut(s)	719
AciI	CCGC	6 cut(s)	467, 498, 1166, 1181, 1221, 1319
AclWI	GGATC	1 cut(s)	899
AcsI	RAATTY	4 cut(s)	214, 879, 982, 1271
AcuI	CTGAAG	2 cut(s)	130, 800
AfaI	GTAC	2 cut(s)	86, 721
AfiI	CCNNNNNNNGG	2 cut(s)	124, 190
AflII	CTTAAG	1 cut(s)	1394
AflIII	ACRYGT	1 cut(s)	1253
AgeI	ACCGGT	2 cut(s)	794, 1518
AgsI	TTSAA	6 cut(s)	20, 220, 304, 805, 863, 1010
AhlI	ACTAGT	1 cut(s)	995
AjnI	CCWGG	1 cut(s)	431
AjuI	GAANNNNNNNTTGG	2 cut(s)	1439, 1471
AluBI	AGCT	7 cut(s)	56, 122, 142, 272, 427, 1244, 1424
AluI	AGCT	7 cut(s)	56, 122, 142, 272, 427, 1244, 1424
Alw21I	GWGCWC	1 cut(s)	144
Alw26I	GTCTC	8 cut(s)	77, 561, 779, 785, 1159, 1218, 1244, 1401
AlwI	GGATC	1 cut(s)	899
AlwNI	CAGNNNCTG	1 cut(s)	692
AoxI	GGCC	1 cut(s)	1384
ApeKI	GCWGC	9 cut(s)	266, 269, 406, 409, 427, 624, 1326, 1424, 1526
ApoI	RAATTY	4 cut(s)	214, 879, 982, 1271
ArsI	GACNNNNNNTTYG	2 cut(s)	635, 667
AsiGI	ACCGGT	2 cut(s)	794, 1518
Asp718I	GGTACC	1 cut(s)	719
AspLEI	GCGC	1 cut(s)	1536
AspS9I	GGNCC	2 cut(s)	361, 490
AsuHPI	GGTGA	7 cut(s)	113, 406, 724, 748, 902, 971, 1200
AsuII	TTCGAA	1 cut(s)	654
AvaII	GGWCC	2 cut(s)	361, 490
BanI	GGYRCC	1 cut(s)	719
BanII	GRGCYC	1 cut(s)	144
BbsI	GAAGAC	1 cut(s)	1493
Bbv12I	GWGCWC	1 cut(s)	144
BbvCI	CCTCAGC	1 cut(s)	123
BbvI	GCAGC	9 cut(s)	253, 281, 414, 418, 421, 611, 1313, 1411, 1513
BccI	CCATC	6 cut(s)	545, 710, 758, 767, 836, 1126
BceAI	ACGGC	1 cut(s)	911
BcgI	CGANNNNNNTGC	2 cut(s)	331, 365
BciT130I	CCWGG	1 cut(s)	433
BciVI	GTATCC	1 cut(s)	1007
BclI	TGATCA	1 cut(s)	919
BcoDI	GTCTC	8 cut(s)	77, 561, 779, 785, 1159, 1218, 1244, 1401
BcuI	ACTAGT	1 cut(s)	995
BfaI	CTAG	3 cut(s)	53, 78, 996
BfmI	CTRYAG	3 cut(s)	267, 625, 1502
BfrI	CTTAAG	1 cut(s)	1394
BfuAI	ACCTGC	1 cut(s)	731
BfuI	GTATCC	1 cut(s)	1007
BisI	GCNGC	9 cut(s)	267, 270, 407, 410, 428, 625, 1327, 1425, 1527
BlsI	GCNGC	9 cut(s)	268, 271, 408, 411, 429, 626, 1328, 1426, 1528
BmcAI	AGTACT	1 cut(s)	86
Bme1390I	CCNGG	1 cut(s)	433
Bme18I	GGWCC	2 cut(s)	361, 490
BmgT120I	GGNCC	2 cut(s)	361, 490
BmiI	GGNNCC	2 cut(s)	535, 721
BmrFI	CCNGG	1 cut(s)	433
BmsI	GCATC	1 cut(s)	427
BpiI	GAAGAC	1 cut(s)	1493
BpmI	CTGGAG	1 cut(s)	552
Bpu10I	CCTNAGC	1 cut(s)	123
Bpu14I	TTCGAA	1 cut(s)	654
Bsa29I	ATCGAT	1 cut(s)	762
BsaI	GGTCTC	3 cut(s)	77, 561, 785
BsaJI	CCNNGG	6 cut(s)	431, 432, 493, 735, 1071, 1460
BsaWI	WCCGGW	4 cut(s)	563, 794, 872, 1518
Bsc4I	CCNNNNNNNGG	2 cut(s)	124, 190
Bse118I	RCCGGY	2 cut(s)	794, 1518
Bse1I	ACTGG	7 cut(s)	27, 396, 535, 561, 974, 1172, 1336
Bse3DI	GCAATG	3 cut(s)	331, 676, 1536
BseBI	CCWGG	1 cut(s)	433
BseCI	ATCGAT	1 cut(s)	762
BseDI	CCNNGG	6 cut(s)	431, 432, 493, 735, 1071, 1460
BseGI	GGATG	2 cut(s)	442, 847
BseLI	CCNNNNNNNGG	2 cut(s)	124, 190
BseMI	GCAATG	3 cut(s)	331, 676, 1536
BseMII	CTCAG	4 cut(s)	114, 244, 302, 1039
BseNI	ACTGG	7 cut(s)	27, 396, 535, 561, 974, 1172, 1336
BseRI	GAGGAG	5 cut(s)	140, 206, 217, 344, 1119
BseXI	GCAGC	9 cut(s)	253, 281, 414, 418, 421, 611, 1313, 1411, 1513
BseYI	CCCAGC	1 cut(s)	118
BsgI	GTGCAG	2 cut(s)	1080, 1312
BshFI	GGCC	1 cut(s)	1386
BshNI	GGYRCC	1 cut(s)	719
BshTI	ACCGGT	2 cut(s)	794, 1518
BshVI	ATCGAT	1 cut(s)	762
BsiHKAI	GWGCWC	1 cut(s)	144
BsiSI	CCGG	4 cut(s)	564, 795, 873, 1519
BslFI	GGGAC	1 cut(s)	398
BslI	CCNNNNNNNGG	2 cut(s)	124, 190
BsmAI	GTCTC	8 cut(s)	77, 561, 779, 785, 1159, 1218, 1244, 1401
BsmFI	GGGAC	1 cut(s)	398
BsmI	GAATGC	2 cut(s)	372, 1321
BsnI	GGCC	1 cut(s)	1386
Bso31I	GGTCTC	3 cut(s)	77, 561, 785
Bsp119I	TTCGAA	1 cut(s)	654
Bsp1286I	GDGCHC	1 cut(s)	144
Bsp143I	GATC	3 cut(s)	372, 904, 919
Bsp19I	CCATGG	2 cut(s)	1071, 1460
BspACI	CCGC	6 cut(s)	467, 498, 1166, 1181, 1221, 1319
BspANI	GGCC	1 cut(s)	1386
BspCNI	CTCAG	4 cut(s)	115, 243, 301, 1038
BspDI	ATCGAT	1 cut(s)	762
BspLI	GGNNCC	2 cut(s)	535, 721
BspMAI	CTGCAG	2 cut(s)	271, 629
BspMI	ACCTGC	1 cut(s)	731
BspPI	GGATC	1 cut(s)	899
BspQI	GCTCTTC	1 cut(s)	149
BspT104I	TTCGAA	1 cut(s)	654
BspT107I	GGYRCC	1 cut(s)	719
BspTI	CTTAAG	1 cut(s)	1394
BspTNI	GGTCTC	3 cut(s)	77, 561, 785
BsrDI	GCAATG	3 cut(s)	331, 676, 1536
BsrFI	RCCGGY	2 cut(s)	794, 1518
BsrI	ACTGG	7 cut(s)	27, 396, 535, 561, 974, 1172, 1336
BssAI	RCCGGY	2 cut(s)	794, 1518
BssECI	CCNNGG	6 cut(s)	431, 432, 493, 735, 1071, 1460
BssMI	GATC	3 cut(s)	372, 904, 919
BssT1I	CCWWGG	4 cut(s)	493, 735, 1071, 1460
Bst2UI	CCWGG	1 cut(s)	433
Bst4CI	ACNGT	4 cut(s)	109, 890, 959, 1506
Bst6I	CTCTTC	3 cut(s)	149, 777, 1427
BstAFI	CTTAAG	1 cut(s)	1394
BstAPI	GCANNNNNTGC	1 cut(s)	1067
BstBI	TTCGAA	1 cut(s)	654
BstC8I	GCNNGC	1 cut(s)	58
BstDEI	CTNAG	4 cut(s)	123, 230, 288, 1025
BstDSI	CCRYGG	2 cut(s)	1071, 1460
BstF5I	GGATG	2 cut(s)	442, 847
BstHHI	GCGC	1 cut(s)	1536
BstKTI	GATC	3 cut(s)	375, 907, 922
BstMAI	GTCTC	8 cut(s)	77, 561, 779, 785, 1159, 1218, 1244, 1401
BstMBI	GATC	3 cut(s)	372, 904, 919
BstMWI	GCNNNNNNNGC	5 cut(s)	53, 148, 633, 675, 1067
BstNI	CCWGG	1 cut(s)	433
BstNSI	RCATGY	2 cut(s)	254, 1257
BstSCI	CCNGG	1 cut(s)	431
BstSFI	CTRYAG	3 cut(s)	267, 625, 1502
BstV1I	GCAGC	9 cut(s)	253, 281, 414, 418, 421, 611, 1313, 1411, 1513
BstV2I	GAAGAC	1 cut(s)	1493
Bsu15I	ATCGAT	1 cut(s)	762
BsuI	GTATCC	1 cut(s)	1007
BsuRI	GGCC	1 cut(s)	1386
BsuTUI	ATCGAT	1 cut(s)	762
BtgI	CCRYGG	2 cut(s)	1071, 1460
BtsCI	GGATG	2 cut(s)	442, 847
BtsI	GCAGTG	1 cut(s)	634
BtsIMutI	CAGTG	7 cut(s)	114, 528, 554, 634, 1179, 1329, 1511
BveI	ACCTGC	1 cut(s)	731
Cac8I	GCNNGC	1 cut(s)	58
CaiI	CAGNNNCTG	1 cut(s)	692
CfoI	GCGC	1 cut(s)	1536
Cfr10I	RCCGGY	2 cut(s)	794, 1518
Cfr13I	GGNCC	2 cut(s)	361, 490
ClaI	ATCGAT	1 cut(s)	762
Csp6I	GTAC	2 cut(s)	85, 720
CspAI	ACCGGT	2 cut(s)	794, 1518
CviQI	GTAC	2 cut(s)	85, 720
DdeI	CTNAG	4 cut(s)	123, 230, 288, 1025
DpnI	GATC	3 cut(s)	374, 906, 921
DpnII	GATC	3 cut(s)	372, 904, 919
Eam1104I	CTCTTC	3 cut(s)	149, 777, 1427
EarI	CTCTTC	3 cut(s)	149, 777, 1427
Ecl136II	GAGCTC	1 cut(s)	142
Eco130I	CCWWGG	4 cut(s)	493, 735, 1071, 1460
Eco147I	AGGCCT	1 cut(s)	1386
Eco24I	GRGCYC	1 cut(s)	144
Eco31I	GGTCTC	3 cut(s)	77, 561, 785
Eco32I	GATATC	1 cut(s)	1375
Eco47I	GGWCC	2 cut(s)	361, 490
Eco53kI	GAGCTC	1 cut(s)	142
Eco57I	CTGAAG	2 cut(s)	130, 800
EcoICRI	GAGCTC	1 cut(s)	142
EcoRI	GAATTC	2 cut(s)	982, 1271
EcoRII	CCWGG	1 cut(s)	431
EcoRV	GATATC	1 cut(s)	1375
EcoT14I	CCWWGG	4 cut(s)	493, 735, 1071, 1460
EcoT38I	GRGCYC	1 cut(s)	144
ErhI	CCWWGG	4 cut(s)	493, 735, 1071, 1460
FaqI	GGGAC	1 cut(s)	398
FauI	CCCGC	1 cut(s)	460
FauNDI	CATATG	1 cut(s)	1048
FbaI	TGATCA	1 cut(s)	919
Fnu4HI	GCNGC	9 cut(s)	267, 270, 407, 410, 428, 625, 1327, 1425, 1527
FokI	GGATG	2 cut(s)	449, 854
FriOI	GRGCYC	1 cut(s)	144
Fsp4HI	GCNGC	9 cut(s)	267, 270, 407, 410, 428, 625, 1327, 1425, 1527
FspBI	CTAG	3 cut(s)	53, 78, 996
GlaI	GCGC	1 cut(s)	1535
GluI	GCNGC	9 cut(s)	267, 270, 407, 410, 428, 625, 1327, 1425, 1527
GsaI	CCCAGC	1 cut(s)	122
GsuI	CTGGAG	1 cut(s)	552
HaeIII	GGCC	1 cut(s)	1386
HapII	CCGG	4 cut(s)	564, 795, 873, 1519
HhaI	GCGC	1 cut(s)	1536
Hin6I	GCGC	1 cut(s)	1534
HinP1I	GCGC	1 cut(s)	1534
HinfI	GANTC	4 cut(s)	226, 294, 689, 1094
HpaII	CCGG	4 cut(s)	564, 795, 873, 1519
HphI	GGTGA	7 cut(s)	113, 406, 724, 748, 902, 971, 1200
Hpy166II	GTNNAC	1 cut(s)	112
Hpy188I	TCNGA	7 cut(s)	291, 365, 586, 694, 901, 952, 1028
Hpy188III	TCNNGA	1 cut(s)	220
Hpy8I	GTNNAC	1 cut(s)	112
Hpy99I	CGWCG	1 cut(s)	905
HpyAV	CCTTC	7 cut(s)	457, 536, 614, 1004, 1023, 1215, 1376
HpyCH4III	ACNGT	4 cut(s)	109, 890, 959, 1506
HpyCH4IV	ACGT	1 cut(s)	729
HpyF10VI	GCNNNNNNNGC	5 cut(s)	53, 148, 633, 675, 1067
HpyF3I	CTNAG	4 cut(s)	123, 230, 288, 1025
HpySE526I	ACGT	1 cut(s)	729
HspAI	GCGC	1 cut(s)	1534
KpnI	GGTACC	1 cut(s)	723
Ksp22I	TGATCA	1 cut(s)	919
Kzo9I	GATC	3 cut(s)	372, 904, 919
LguI	GCTCTTC	1 cut(s)	149
LmnI	GCTCC	6 cut(s)	139, 193, 445, 533, 586, 902
Lsp1109I	GCAGC	9 cut(s)	253, 281, 414, 418, 421, 611, 1313, 1411, 1513
LweI	GCATC	1 cut(s)	427
MaeI	CTAG	3 cut(s)	53, 78, 996
MaeII	ACGT	1 cut(s)	729
MaeIII	GTNAC	6 cut(s)	475, 605, 730, 754, 1174, 1355
MalI	GATC	3 cut(s)	374, 906, 921
MboI	GATC	3 cut(s)	372, 904, 919
MboII	GAAGA	7 cut(s)	136, 516, 794, 974, 1444, 1498, 1522
MhlI	GDGCHC	1 cut(s)	144
MluCI	AATT	7 cut(s)	15, 214, 471, 863, 879, 982, 1271
MlyI	GAGTC	2 cut(s)	303, 1103
MmeI	TCCRAC	3 cut(s)	222, 924, 1148
MseI	TTAA	3 cut(s)	276, 486, 1395
MspA1I	CMGCKG	2 cut(s)	122, 1244
MspCI	CTTAAG	1 cut(s)	1394
MspI	CCGG	4 cut(s)	564, 795, 873, 1519
MspR9I	CCNGG	1 cut(s)	433
Mva1269I	GAATGC	2 cut(s)	372, 1321
MvaI	CCWGG	1 cut(s)	433
MwoI	GCNNNNNNNGC	5 cut(s)	53, 148, 633, 675, 1067
NcoI	CCATGG	2 cut(s)	1071, 1460
NdeI	CATATG	1 cut(s)	1048
NdeII	GATC	3 cut(s)	372, 904, 919
NlaIV	GGNNCC	2 cut(s)	535, 721
NmuCI	GTSAC	6 cut(s)	475, 605, 730, 754, 1174, 1355
NspI	RCATGY	2 cut(s)	254, 1257
NspV	TTCGAA	1 cut(s)	654
PasI	CCCWGGG	1 cut(s)	432
PceI	AGGCCT	1 cut(s)	1386
PciI	ACATGT	1 cut(s)	1253
PciSI	GCTCTTC	1 cut(s)	149
PctI	GAATGC	2 cut(s)	372, 1321
PfeI	GAWTC	2 cut(s)	226, 689
PinAI	ACCGGT	2 cut(s)	794, 1518
PkrI	GCNGC	9 cut(s)	268, 271, 408, 411, 429, 626, 1328, 1426, 1528
PleI	GAGTC	2 cut(s)	302, 1102
PpsI	GAGTC	2 cut(s)	302, 1102
PscI	ACATGT	1 cut(s)	1253
Psp124BI	GAGCTC	1 cut(s)	144
Psp6I	CCWGG	1 cut(s)	431
PspFI	CCCAGC	1 cut(s)	118
PspGI	CCWGG	1 cut(s)	431
PspN4I	GGNNCC	2 cut(s)	535, 721
PspPI	GGNCC	2 cut(s)	361, 490
PstI	CTGCAG	2 cut(s)	271, 629
PstNI	CAGNNNCTG	1 cut(s)	692
PvuII	CAGCTG	2 cut(s)	122, 1244
RsaI	GTAC	2 cut(s)	86, 721
RsaNI	GTAC	2 cut(s)	85, 720
SacI	GAGCTC	1 cut(s)	144
SapI	GCTCTTC	1 cut(s)	149
SaqAI	TTAA	3 cut(s)	276, 486, 1395
SatI	GCNGC	9 cut(s)	267, 270, 407, 410, 428, 625, 1327, 1425, 1527
Sau3AI	GATC	3 cut(s)	372, 904, 919
Sau96I	GGNCC	2 cut(s)	361, 490
ScaI	AGTACT	1 cut(s)	86
SchI	GAGTC	2 cut(s)	303, 1103
ScrFI	CCNGG	1 cut(s)	433
SduI	GDGCHC	1 cut(s)	144
SfaNI	GCATC	1 cut(s)	427
SfcI	CTRYAG	3 cut(s)	267, 625, 1502
SfuI	TTCGAA	1 cut(s)	654
SinI	GGWCC	2 cut(s)	361, 490
SmlI	CTYRAG	1 cut(s)	1394
SmoI	CTYRAG	1 cut(s)	1394
SpeI	ACTAGT	1 cut(s)	995
Sse9I	AATT	7 cut(s)	15, 214, 471, 863, 879, 982, 1271
SseBI	AGGCCT	1 cut(s)	1386
SsiI	CCGC	6 cut(s)	467, 498, 1166, 1181, 1221, 1319
SspMI	CTAG	3 cut(s)	53, 78, 996
SstI	GAGCTC	1 cut(s)	144
StuI	AGGCCT	1 cut(s)	1386
StyD4I	CCNGG	1 cut(s)	431
StyI	CCWWGG	4 cut(s)	493, 735, 1071, 1460
TaaI	ACNGT	4 cut(s)	109, 890, 959, 1506
TaiI	ACGT	1 cut(s)	732
TaqI	TCGA	5 cut(s)	540, 654, 762, 780, 1377
TasI	AATT	7 cut(s)	15, 214, 471, 863, 879, 982, 1271
TatI	WGTACW	1 cut(s)	84
TfiI	GAWTC	2 cut(s)	226, 689
Tru1I	TTAA	3 cut(s)	276, 486, 1395
Tru9I	TTAA	3 cut(s)	276, 486, 1395
TscAI	CASTG	7 cut(s)	114, 535, 561, 634, 1179, 1336, 1511
TseFI	GTSAC	6 cut(s)	475, 605, 730, 754, 1174, 1355
TseI	GCWGC	9 cut(s)	266, 269, 406, 409, 427, 624, 1326, 1424, 1526
Tsp45I	GTSAC	6 cut(s)	475, 605, 730, 754, 1174, 1355
TspDTI	ATGAA	5 cut(s)	28, 975, 1068, 1284, 1529
TspRI	CASTG	7 cut(s)	114, 535, 561, 634, 1179, 1336, 1511
Vha464I	CTTAAG	1 cut(s)	1394
VpaK11BI	GGWCC	2 cut(s)	361, 490
XapI	RAATTY	4 cut(s)	214, 879, 982, 1271
XceI	RCATGY	2 cut(s)	254, 1257
XcmI	CCANNNNNNNNNTGG	1 cut(s)	500
XspI	CTAG	3 cut(s)	53, 78, 996
ZrmI	AGTACT	1 cut(s)	86

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.