RchiOBHm_Chr5g0049631

Double-stranded RNA binding motif

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0016795
Physical Location & Seq
Reverse (-)
47418566 .. 47420815
2250 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1077 bp
ATGATGTACAAGAGTAAGCTGCAGGAGCTGTGCCATGAAAACAAATGGGGTTTGCCAAGATACACTGCCATGAAAGATGGACCGGATCACAATCCTTGCTTCAAGGCTTGTGTTTCAGTAAATGGCTACTCCTTTGACTCGCCGGTTACTTGTAAATCTTTCAAACAAGCCCAGAACCAAGCTGCCATGATCGCTTTTCTTCACTTCACCTCTCCACCTTCTTCTTCTTCTTCACTTTCACCGGTGGATGAGCATAGCACCTCGGAGAGCCCAGAGTTCAACACTACTAGTACTTTAAAGAAGGCAGAGAATGCTGCTTCTATGTCTATTTCATTCTCAGACGGGTTTCAGGATGACGAACCTGGTCGTCTTTACAAGAATCTCTTACAGGAGTTGGCTCAGAGAGAAGGTTTTTCCATGCCAGCATATACAACTGTGAACTCGGGTGCATTTCACATGCCAACATTCTATTCCACTGTGGATGTGGAAGGAGAGAAATTCTCCGGACATGTGGGGAAATCCAAAAAACAGGCAGAGTTGAGTGCCGCGAAGGTTGCTTACACCACCTTAAAAGAGCGTGCTCTCAAAACCACTCAAAGCACTAACTGGAGAATGAATGTTGTTCCTGAACAAGATTTGGTTTCCTCTCCAACTACCAAGAATAAGGAATGTGCTTTAGAAACTAAAGAGATCGATGAAGTTAAAGGGTCAGACAATGTATTGGACAACATCAATCTCAAAGCTGTGTACTGCAATGCACCTGATGCAATGGTACTGGAAATCGATAATATGAATAAAACTGGAAAGCCTTCCTCATGTTTGGAAACAGTGCCCTCTTCAACTAAAGAATACCCTTCCTCTCCAGCATTTATGCATACTGATCTTTCGGCTATCTCAATTGTGGATAAAAATGAAGGGAAATTAACGGGATTGAAAAGTTACTTGCTGTGCAACAGGTTTAGAGTCTACACACATTTTCCAGATATTGCATTCCCCAAGTGCATAACTGTGCTGCCCATCAGTGATGACAAATGGGTGGCTGTCAGGTTGGAGTTCCCAAATGAAGAAAGCAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
dsrm

Protein Analysis

358

Amino Acids

39.69

Weight (kDa)

5.82

Isoelectric Point (pI)

46.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
dsrm PF00035 3 - 66 1.6e-11 Double-stranded RNA binding motif
dsrm PF00035 125 - 188 1.4e-13 Double-stranded RNA binding motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016795)

Species Orthologous Gene IDs
fragaria_vesca FvH4_3g27052
malus_domestica MD11G1178800.v1.1
prunus_persica Prupe.6G151600_v2.0.a1 Prupe.6G151600_v2.0.a1
rosa_chinensis RchiOBHm_Chr5g0049631
rosa_laevigata RLG00000034628
rosa_multiflora Rmu_sc0013777.1_g000008
rosa_roxburghii Rroxscaffold_1G00032040
rosa_rugosa Rorug05G0249300
rosa_samantha Rh5AG325800 Rh5BG337100 Rh5CG361800 Rh5DG349300
rosa_wichuraiana Rw5G030790

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 966
AccII CGCG 1 cut(s) 548
AccIII TCCGGA 1 cut(s) 503
AciI CCGC 1 cut(s) 546
AclWI GGATC 1 cut(s) 93
AcsI RAATTY 1 cut(s) 497
AfaI GTAC 4 cut(s) 8, 292, 749, 774
AflIII ACRYGT 1 cut(s) 508
AgeI ACCGGT 1 cut(s) 241
AgsI TTSAA 5 cut(s) 103, 163, 280, 840, 934
AhlI ACTAGT 1 cut(s) 287
AjnI CCWGG 1 cut(s) 361
AjuI GAANNNNNNNTTGG 2 cut(s) 454, 486
AluBI AGCT 4 cut(s) 19, 28, 182, 743
AluI AGCT 4 cut(s) 19, 28, 182, 743
Alw21I GWGCWC 1 cut(s) 583
AlwI GGATC 1 cut(s) 93
AlwNI CAGNNNCTG 1 cut(s) 28
Ama87I CYCGRG 1 cut(s) 442
Aor13HI TCCGGA 1 cut(s) 503
ApeKI GCWGC 4 cut(s) 19, 182, 314, 1012
ApoI RAATTY 1 cut(s) 497
AsiGI ACCGGT 1 cut(s) 241
Asp700I GAANNNNTTC 1 cut(s) 808
AspS9I GGNCC 1 cut(s) 80
AsuHPI GGTGA 2 cut(s) 199, 231
AvaI CYCGRG 1 cut(s) 442
AvaII GGWCC 1 cut(s) 80
BaeGI GKGCMC 1 cut(s) 834
BanII GRGCYC 1 cut(s) 272
BarI GAAGNNNNNNTAC 2 cut(s) 542, 574
Bbv12I GWGCWC 1 cut(s) 583
BbvI GCAGC 4 cut(s) 6, 169, 301, 999
BccI CCATC 2 cut(s) 71, 1025
BciT130I CCWGG 1 cut(s) 363
BcuI ACTAGT 1 cut(s) 287
BfaI CTAG 1 cut(s) 288
BfmI CTRYAG 1 cut(s) 20
BisI GCNGC 5 cut(s) 20, 183, 315, 546, 1013
BlsI GCNGC 5 cut(s) 21, 184, 316, 547, 1014
BmcAI AGTACT 1 cut(s) 292
Bme1390I CCNGG 1 cut(s) 363
Bme18I GGWCC 1 cut(s) 80
BmeT110I CYCGRG 1 cut(s) 442
BmgT120I GGNCC 1 cut(s) 80
BmrFI CCNGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 754
BplI GAGNNNNNCTC 2 cut(s) 485, 517
BpmI CTGGAG 2 cut(s) 628, 846
Bsa29I ATCGAT 2 cut(s) 693, 783
BsaBI GATNNNNATC 1 cut(s) 90
BsaJI CCNNGG 1 cut(s) 261
BsaWI WCCGGW 3 cut(s) 82, 241, 503
Bse118I RCCGGY 2 cut(s) 142, 241
Bse1I ACTGG 3 cut(s) 611, 780, 805
Bse3DI GCAATG 2 cut(s) 760, 774
Bse8I GATNNNNATC 1 cut(s) 90
BseAI TCCGGA 1 cut(s) 503
BseBI CCWGG 1 cut(s) 363
BseCI ATCGAT 2 cut(s) 693, 783
BseDI CCNNGG 1 cut(s) 261
BseGI GGATG 3 cut(s) 253, 358, 487
BseJI GATNNNNATC 1 cut(s) 90
BseMI GCAATG 2 cut(s) 760, 774
BseMII CTCAG 2 cut(s) 351, 413
BseNI ACTGG 3 cut(s) 611, 780, 805
BseSI GKGCMC 1 cut(s) 834
BseXI GCAGC 4 cut(s) 6, 169, 301, 999
Bsh1236I CGCG 1 cut(s) 548
BshTI ACCGGT 1 cut(s) 241
BshVI ATCGAT 2 cut(s) 693, 783
BsiHKAI GWGCWC 1 cut(s) 583
BsiHKCI CYCGRG 1 cut(s) 442
BsiSI CCGG 4 cut(s) 83, 143, 242, 504
BsmI GAATGC 2 cut(s) 316, 989
BsoBI CYCGRG 1 cut(s) 442
Bsp1286I GDGCHC 3 cut(s) 272, 583, 834
Bsp13I TCCGGA 1 cut(s) 503
Bsp1407I TGTACA 1 cut(s) 6
Bsp143I GATC 4 cut(s) 85, 189, 690, 880
BspACI CCGC 1 cut(s) 546
BspCNI CTCAG 2 cut(s) 350, 412
BspDI ATCGAT 2 cut(s) 693, 783
BspEI TCCGGA 1 cut(s) 503
BspFNI CGCG 1 cut(s) 548
BspMAI CTGCAG 1 cut(s) 24
BspPI GGATC 1 cut(s) 93
BsrDI GCAATG 2 cut(s) 760, 774
BsrFI RCCGGY 2 cut(s) 142, 241
BsrGI TGTACA 1 cut(s) 6
BsrI ACTGG 3 cut(s) 611, 780, 805
BssAI RCCGGY 2 cut(s) 142, 241
BssECI CCNNGG 1 cut(s) 261
BssMI GATC 4 cut(s) 85, 189, 690, 880
Bst2UI CCWGG 1 cut(s) 363
Bst4CI ACNGT 4 cut(s) 436, 478, 829, 1009
Bst6I CTCTTC 1 cut(s) 841
BstAPI GCANNNNNTGC 2 cut(s) 311, 764
BstAUI TGTACA 1 cut(s) 6
BstC8I GCNNGC 2 cut(s) 423, 579
BstDEI CTNAG 2 cut(s) 337, 399
BstF5I GGATG 3 cut(s) 253, 358, 487
BstFNI CGCG 1 cut(s) 548
BstKTI GATC 4 cut(s) 88, 192, 693, 883
BstMBI GATC 4 cut(s) 85, 189, 690, 880
BstMWI GCNNNNNNNGC 5 cut(s) 25, 191, 311, 554, 764
BstNI CCWGG 1 cut(s) 363
BstNSI RCATGY 2 cut(s) 460, 512
BstSCI CCNGG 1 cut(s) 361
BstSFI CTRYAG 1 cut(s) 20
BstSLI GKGCMC 1 cut(s) 834
BstUI CGCG 1 cut(s) 548
BstV1I GCAGC 4 cut(s) 6, 169, 301, 999
Bsu15I ATCGAT 2 cut(s) 693, 783
BsuTUI ATCGAT 2 cut(s) 693, 783
BtsCI GGATG 3 cut(s) 253, 358, 487
BtsI GCAGTG 1 cut(s) 63
BtsIMutI CAGTG 4 cut(s) 63, 474, 834, 1027
Cac8I GCNNGC 2 cut(s) 423, 579
CaiI CAGNNNCTG 1 cut(s) 28
Cfr10I RCCGGY 2 cut(s) 142, 241
Cfr13I GGNCC 1 cut(s) 80
ClaI ATCGAT 2 cut(s) 693, 783
CsiI ACCWGGT 1 cut(s) 361
Csp6I GTAC 4 cut(s) 7, 291, 748, 773
CspAI ACCGGT 1 cut(s) 241
CviAII CATG 7 cut(s) 35, 70, 187, 418, 457, 509, 816
CviQI GTAC 4 cut(s) 7, 291, 748, 773
DdeI CTNAG 2 cut(s) 337, 399
DpnI GATC 4 cut(s) 87, 191, 692, 882
DpnII GATC 4 cut(s) 85, 189, 690, 880
DraI TTTAAA 1 cut(s) 297
Eam1104I CTCTTC 1 cut(s) 841
EarI CTCTTC 1 cut(s) 841
Eco24I GRGCYC 1 cut(s) 272
Eco47I GGWCC 1 cut(s) 80
Eco88I CYCGRG 1 cut(s) 442
EcoRII CCWGG 1 cut(s) 361
EcoT22I ATGCAT 1 cut(s) 876
EcoT38I GRGCYC 1 cut(s) 272
FaeI CATG 7 cut(s) 38, 73, 190, 421, 460, 512, 819
FalI AAGNNNNNCTT 2 cut(s) 368, 400
FatI CATG 7 cut(s) 34, 69, 186, 417, 456, 508, 815
FblI GTMKAC 1 cut(s) 966
Fnu4HI GCNGC 5 cut(s) 20, 183, 315, 546, 1013
FokI GGATG 3 cut(s) 260, 365, 494
FriOI GRGCYC 1 cut(s) 272
Fsp4HI GCNGC 5 cut(s) 20, 183, 315, 546, 1013
FspBI CTAG 1 cut(s) 288
GluI GCNGC 5 cut(s) 20, 183, 315, 546, 1013
GsuI CTGGAG 2 cut(s) 628, 846
HapII CCGG 4 cut(s) 83, 143, 242, 504
Hin1II CATG 7 cut(s) 38, 73, 190, 421, 460, 512, 819
HinfI GANTC 3 cut(s) 137, 379, 963
HpaII CCGG 4 cut(s) 83, 143, 242, 504
HphI GGTGA 2 cut(s) 199, 231
Hpy166II GTNNAC 3 cut(s) 439, 748, 967
Hpy188I TCNGA 4 cut(s) 265, 340, 402, 712
Hpy188III TCNNGA 4 cut(s) 350, 504, 626, 980
Hpy8I GTNNAC 3 cut(s) 439, 748, 967
HpyAV CCTTC 8 cut(s) 228, 295, 401, 482, 544, 819, 864, 908
HpyCH4III ACNGT 4 cut(s) 436, 478, 829, 1009
HpyCH4V TGCA 9 cut(s) 22, 449, 753, 758, 767, 874, 951, 989, 1002
HpyF10VI GCNNNNNNNGC 5 cut(s) 25, 191, 311, 554, 764
HpyF3I CTNAG 2 cut(s) 337, 399
Hsp92II CATG 7 cut(s) 38, 73, 190, 421, 460, 512, 819
Kpn2I TCCGGA 1 cut(s) 503
Kzo9I GATC 4 cut(s) 85, 189, 690, 880
LmnI GCTCC 1 cut(s) 25
Lsp1109I GCAGC 4 cut(s) 6, 169, 301, 999
LweI GCATC 1 cut(s) 754
MabI ACCWGGT 1 cut(s) 361
MaeI CTAG 1 cut(s) 288
MaeIII GTNAC 2 cut(s) 145, 938
MalI GATC 4 cut(s) 87, 191, 692, 882
MboI GATC 4 cut(s) 85, 189, 690, 880
MboII GAAGA 7 cut(s) 191, 213, 216, 219, 222, 828, 1076
MfeI CAATTG 1 cut(s) 897
MhlI GDGCHC 3 cut(s) 272, 583, 834
MluCI AATT 4 cut(s) 497, 897, 920, 1072
MlyI GAGTC 2 cut(s) 131, 972
MmeI TCCRAC 2 cut(s) 674, 1029
MnlI CCTC 6 cut(s) 220, 271, 655, 823, 844, 868
Mph1103I ATGCAT 1 cut(s) 876
MroI TCCGGA 1 cut(s) 503
MroXI GAANNNNTTC 1 cut(s) 808
MseI TTAA 4 cut(s) 296, 569, 702, 923
MslI CAYNNNNRTG 2 cut(s) 68, 1007
MspI CCGG 4 cut(s) 83, 143, 242, 504
MspR9I CCNGG 1 cut(s) 363
MunI CAATTG 1 cut(s) 897
Mva1269I GAATGC 2 cut(s) 316, 989
MvaI CCWGG 1 cut(s) 363
MvnI CGCG 1 cut(s) 548
MwoI GCNNNNNNNGC 5 cut(s) 25, 191, 311, 554, 764
NdeII GATC 4 cut(s) 85, 189, 690, 880
NlaIII CATG 7 cut(s) 38, 73, 190, 421, 460, 512, 819
NsiI ATGCAT 1 cut(s) 876
NspI RCATGY 2 cut(s) 460, 512
PciI ACATGT 1 cut(s) 508
PctI GAATGC 2 cut(s) 316, 989
PdmI GAANNNNTTC 1 cut(s) 808
PfeI GAWTC 1 cut(s) 379
PinAI ACCGGT 1 cut(s) 241
PkrI GCNGC 5 cut(s) 21, 184, 316, 547, 1014
PleI GAGTC 2 cut(s) 131, 971
PpsI GAGTC 2 cut(s) 131, 971
PscI ACATGT 1 cut(s) 508
Psp6I CCWGG 1 cut(s) 361
PspGI CCWGG 1 cut(s) 361
PspPI GGNCC 1 cut(s) 80
PstI CTGCAG 1 cut(s) 24
PstNI CAGNNNCTG 1 cut(s) 28
RsaI GTAC 4 cut(s) 8, 292, 749, 774
RsaNI GTAC 4 cut(s) 7, 291, 748, 773
RseI CAYNNNNRTG 2 cut(s) 68, 1007
SaqAI TTAA 4 cut(s) 296, 569, 702, 923
SatI GCNGC 5 cut(s) 20, 183, 315, 546, 1013
Sau3AI GATC 4 cut(s) 85, 189, 690, 880
Sau96I GGNCC 1 cut(s) 80
ScaI AGTACT 1 cut(s) 292
SchI GAGTC 2 cut(s) 131, 972
ScrFI CCNGG 1 cut(s) 363
SduI GDGCHC 3 cut(s) 272, 583, 834
SexAI ACCWGGT 1 cut(s) 361
SfaNI GCATC 1 cut(s) 754
SfcI CTRYAG 1 cut(s) 20
SgrAI CRCCGGYG 1 cut(s) 241
SinI GGWCC 1 cut(s) 80
SmiMI CAYNNNNRTG 2 cut(s) 68, 1007
SpeI ACTAGT 1 cut(s) 287
Sse9I AATT 4 cut(s) 497, 897, 920, 1072
SsiI CCGC 1 cut(s) 546
SspMI CTAG 1 cut(s) 288
StyD4I CCNGG 1 cut(s) 361
TaaI ACNGT 4 cut(s) 436, 478, 829, 1009
TaqI TCGA 2 cut(s) 693, 783
TasI AATT 4 cut(s) 497, 897, 920, 1072
TatI WGTACW 3 cut(s) 6, 290, 747
TauI GCSGC 1 cut(s) 548
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 4 cut(s) 296, 569, 702, 923
Tru9I TTAA 4 cut(s) 296, 569, 702, 923
TscAI CASTG 4 cut(s) 70, 481, 834, 1027
TseI GCWGC 4 cut(s) 19, 182, 314, 1012
TspDTI ATGAA 8 cut(s) 51, 86, 321, 629, 711, 806, 927, 1077
TspRI CASTG 4 cut(s) 70, 481, 834, 1027
VpaK11BI GGWCC 1 cut(s) 80
XapI RAATTY 1 cut(s) 497
XceI RCATGY 2 cut(s) 460, 512
XcmI CCANNNNNNNNNTGG 1 cut(s) 481
XmiI GTMKAC 1 cut(s) 966
XmnI GAANNNNTTC 1 cut(s) 808
XspI CTAG 1 cut(s) 288
ZrmI AGTACT 1 cut(s) 292
Zsp2I ATGCAT 1 cut(s) 876
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.