RchiOBHm_Chr5g0080691

Ribosomal protein S12/S23

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0010742
Physical Location & Seq
Forward (+)
86690488 .. 86694128
3641 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 900 bp
ATGTACCGCATAGCTTCATCCTTCGCCTCTTCAACTCCTAAGAAGCTGCAGGTCCTCCGCGGTATTAGGGTTTCATCCAATAGGAACTATGTGGCGCAAGGTCTCACCTTCCACATTGGGCCTCGCGCCGCCTTGTTTGAGGTTGCCGACGCTGCCAAGTTCACCATGGCCGCTCTCCTGCAACGCGTCTCTCACCTCGCGGAGGCCTACGGAGTTACCATGGGATCAAAGGATGCCAAGGGCATTGGTTGTTATGAGGACAAGTACAAGAATGCGAGCGAAACGCTTGCGAAACAGGCTACTAATACTGCTGGAGGAGATGGTACAACTTCTGCTGCTGTCTTGACTAAGCAGGCAACAGTCACAGAATGTTGCCAGTCCATAGGTGCTGGGGTTCCCTCTATTTTTGCAAATGGTCAACGAGAGATTGGAGAGTTCTTAGCAAGGGCATTGGAGAACGTTGAAAAGAAAGGGCATGTTATAAGTGAAGATAAAAGTTTCAGTATCGACAGACTGCTCATGTCACCCACATTAAATCAATTGATCCGTCATAATAGAACTGAGAAAAGTCGCACGAACCGTAATCGAGCTTTGGATAAATGCCCCCAAAAGCAAGGAGTATGCCTACGGGTTTTTACGAGAACACCCAAGAAGCCAAATTCAGCTCTGCGTAAGGTAGCCAAAGTAAGGCTAAGCAATCGAAATGATGTATTTTCTTACATTCCAGGTGAAGGTCATAATTTGCAGGAACATTCTATGGTCTTAATAAGAGGGGGCAGAGTGAAAGATTTGCCAGGTGTGAAATTCCATTGTATCAGAGGAGTTAAGGATTTGCTGGGACTTCCAAACCGAAGGAGAGGCCGATCAAAATATGGCGCAGAGAAGCCCAAGAGTGCCTGA

Protein Analysis

299

Amino Acids

32.77

Weight (kDa)

10.22

Isoelectric Point (pI)

36.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 193 - 297 7.9e-37 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 482
Acc36I ACCTGC 1 cut(s) 40
AccBSI CCGCTC 1 cut(s) 173
AccII CGCG 4 cut(s) 60, 126, 186, 200
AciI CCGC 6 cut(s) 7, 58, 60, 129, 171, 200
AclI AACGTT 1 cut(s) 459
AclWI GGATC 2 cut(s) 232, 538
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 2 cut(s) 658, 803
AfaI GTAC 3 cut(s) 5, 266, 325
AfiI CCNNNNNNNGG 3 cut(s) 202, 687, 731
AflIII ACRYGT 1 cut(s) 184
AgsI TTSAA 2 cut(s) 33, 464
AjnI CCWGG 2 cut(s) 724, 793
AluBI AGCT 4 cut(s) 14, 46, 590, 665
AluI AGCT 4 cut(s) 14, 46, 590, 665
Alw26I GTCTC 2 cut(s) 107, 193
AlwI GGATC 2 cut(s) 232, 538
AoxI GGCC 4 cut(s) 119, 168, 204, 859
ApeKI GCWGC 3 cut(s) 46, 152, 335
ApoI RAATTY 2 cut(s) 658, 803
AspLEI GCGC 3 cut(s) 97, 128, 878
AspS9I GGNCC 2 cut(s) 52, 119
AsuHPI GGTGA 5 cut(s) 97, 154, 185, 516, 740
AvaII GGWCC 1 cut(s) 52
BbvI GCAGC 3 cut(s) 33, 139, 322
BccI CCATC 1 cut(s) 314
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BciT130I CCWGG 2 cut(s) 726, 795
BcoDI GTCTC 2 cut(s) 107, 193
BfmI CTRYAG 1 cut(s) 47
BfuAI ACCTGC 1 cut(s) 40
BisI GCNGC 5 cut(s) 47, 129, 153, 171, 336
BlpI GCTNAGC 1 cut(s) 692
BlsI GCNGC 5 cut(s) 48, 130, 154, 172, 337
Bme1390I CCNGG 2 cut(s) 726, 795
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 52, 119
BmiI GGNNCC 1 cut(s) 396
BmrFI CCNGG 2 cut(s) 726, 795
BmsI GCATC 1 cut(s) 223
BpmI CTGGAG 1 cut(s) 333
Bpu1102I GCTNAGC 1 cut(s) 692
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 4 cut(s) 58, 165, 219, 237
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 687, 731
Bse1I ACTGG 1 cut(s) 376
BseBI CCWGG 2 cut(s) 726, 795
BseDI CCNNGG 4 cut(s) 58, 165, 219, 237
BseGI GGATG 3 cut(s) 17, 74, 238
BseLI CCNNNNNNNGG 3 cut(s) 202, 687, 731
BseMII CTCAG 1 cut(s) 552
BseNI ACTGG 1 cut(s) 376
BseRI GAGGAG 2 cut(s) 330, 834
BseXI GCAGC 3 cut(s) 33, 139, 322
BseYI CCCAGC 2 cut(s) 389, 835
Bsh1236I CGCG 4 cut(s) 60, 126, 186, 200
BshFI GGCC 4 cut(s) 121, 170, 206, 861
BslFI GGGAC 1 cut(s) 852
BslI CCNNNNNNNGG 3 cut(s) 202, 687, 731
BsmAI GTCTC 2 cut(s) 107, 193
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 852
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 4 cut(s) 121, 170, 206, 861
Bso31I GGTCTC 1 cut(s) 107
Bsp143I GATC 3 cut(s) 224, 543, 863
Bsp1720I GCTNAGC 1 cut(s) 692
Bsp19I CCATGG 2 cut(s) 165, 219
BspACI CCGC 6 cut(s) 7, 58, 60, 129, 171, 200
BspANI GGCC 4 cut(s) 121, 170, 206, 861
BspCNI CTCAG 1 cut(s) 553
BspFNI CGCG 4 cut(s) 60, 126, 186, 200
BspLI GGNNCC 1 cut(s) 396
BspMAI CTGCAG 1 cut(s) 51
BspMI ACCTGC 1 cut(s) 40
BspPI GGATC 2 cut(s) 232, 538
BspTNI GGTCTC 1 cut(s) 107
BsrBI CCGCTC 1 cut(s) 173
BsrI ACTGG 1 cut(s) 376
BssECI CCNNGG 4 cut(s) 58, 165, 219, 237
BssMI GATC 3 cut(s) 224, 543, 863
BssT1I CCWWGG 3 cut(s) 165, 219, 237
Bst2UI CCWGG 2 cut(s) 726, 795
Bst4CI ACNGT 2 cut(s) 361, 581
Bst6I CTCTTC 1 cut(s) 34
BstC8I GCNNGC 3 cut(s) 277, 288, 354
BstDEI CTNAG 5 cut(s) 39, 348, 439, 561, 692
BstDSI CCRYGG 3 cut(s) 58, 165, 219
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 3 cut(s) 17, 74, 238
BstFNI CGCG 4 cut(s) 60, 126, 186, 200
BstHHI GCGC 3 cut(s) 97, 128, 878
BstKTI GATC 3 cut(s) 227, 546, 866
BstMAI GTCTC 2 cut(s) 107, 193
BstMBI GATC 3 cut(s) 224, 543, 863
BstMWI GCNNNNNNNGC 2 cut(s) 152, 296
BstNI CCWGG 2 cut(s) 726, 795
BstNSI RCATGY 1 cut(s) 479
BstSCI CCNGG 2 cut(s) 724, 793
BstSFI CTRYAG 1 cut(s) 47
BstUI CGCG 4 cut(s) 60, 126, 186, 200
BstV1I GCAGC 3 cut(s) 33, 139, 322
BsuRI GGCC 4 cut(s) 121, 170, 206, 861
BtgI CCRYGG 3 cut(s) 58, 165, 219
BtsCI GGATG 3 cut(s) 17, 74, 238
BveI ACCTGC 1 cut(s) 40
Cac8I GCNNGC 3 cut(s) 277, 288, 354
CfoI GCGC 3 cut(s) 97, 128, 878
Cfr13I GGNCC 2 cut(s) 52, 119
Cfr42I CCGCGG 1 cut(s) 61
CseI GACGC 2 cut(s) 158, 175
Csp6I GTAC 3 cut(s) 4, 265, 324
CviAII CATG 4 cut(s) 166, 220, 476, 520
CviQI GTAC 3 cut(s) 4, 265, 324
DdeI CTNAG 5 cut(s) 39, 348, 439, 561, 692
DpnI GATC 3 cut(s) 226, 545, 865
DpnII GATC 3 cut(s) 224, 543, 863
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
Eco130I CCWWGG 3 cut(s) 165, 219, 237
Eco147I AGGCCT 1 cut(s) 206
Eco31I GGTCTC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 52
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoO109I RGGNCCY 1 cut(s) 52
EcoRII CCWGG 2 cut(s) 724, 793
EcoT14I CCWWGG 3 cut(s) 165, 219, 237
ErhI CCWWGG 3 cut(s) 165, 219, 237
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 4 cut(s) 169, 223, 479, 523
FaqI GGGAC 1 cut(s) 852
FatI CATG 4 cut(s) 165, 219, 475, 519
Fnu4HI GCNGC 5 cut(s) 47, 129, 153, 171, 336
FokI GGATG 3 cut(s) 4, 61, 245
Fsp4HI GCNGC 5 cut(s) 47, 129, 153, 171, 336
GlaI GCGC 3 cut(s) 96, 127, 877
GluI GCNGC 5 cut(s) 47, 129, 153, 171, 336
GsaI CCCAGC 2 cut(s) 393, 839
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 4 cut(s) 121, 170, 206, 861
HgaI GACGC 2 cut(s) 158, 175
HhaI GCGC 3 cut(s) 97, 128, 878
Hin1II CATG 4 cut(s) 169, 223, 479, 523
Hin6I GCGC 3 cut(s) 95, 126, 876
HinP1I GCGC 3 cut(s) 95, 126, 876
HincII GTYRAC 1 cut(s) 419
HindII GTYRAC 1 cut(s) 419
HphI GGTGA 5 cut(s) 97, 154, 185, 516, 740
Hpy166II GTNNAC 2 cut(s) 162, 419
Hpy188I TCNGA 1 cut(s) 818
Hpy188III TCNNGA 1 cut(s) 343
Hpy8I GTNNAC 2 cut(s) 162, 419
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 4 cut(s) 31, 118, 725, 846
HpyCH4III ACNGT 2 cut(s) 361, 581
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 4 cut(s) 49, 181, 410, 745
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 296
HpyF3I CTNAG 5 cut(s) 39, 348, 439, 561, 692
HpySE526I ACGT 1 cut(s) 459
Hsp92II CATG 4 cut(s) 169, 223, 479, 523
HspAI GCGC 3 cut(s) 95, 126, 876
KspI CCGCGG 1 cut(s) 61
Kzo9I GATC 3 cut(s) 224, 543, 863
Lsp1109I GCAGC 3 cut(s) 33, 139, 322
LweI GCATC 1 cut(s) 223
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 3 cut(s) 214, 361, 522
MalI GATC 3 cut(s) 226, 545, 865
MbiI CCGCTC 1 cut(s) 173
MboI GATC 3 cut(s) 224, 543, 863
MboII GAAGA 2 cut(s) 21, 500
MfeI CAATTG 1 cut(s) 539
MluCI AATT 4 cut(s) 539, 658, 739, 803
MluI ACGCGT 1 cut(s) 184
MseI TTAA 3 cut(s) 533, 764, 825
MspA1I CMGCKG 1 cut(s) 60
MspR9I CCNGG 2 cut(s) 726, 795
MunI CAATTG 1 cut(s) 539
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 2 cut(s) 726, 795
MvnI CGCG 4 cut(s) 60, 126, 186, 200
MwoI GCNNNNNNNGC 2 cut(s) 152, 296
NcoI CCATGG 2 cut(s) 165, 219
NdeII GATC 3 cut(s) 224, 543, 863
NlaIII CATG 4 cut(s) 169, 223, 479, 523
NlaIV GGNNCC 1 cut(s) 396
NmuCI GTSAC 2 cut(s) 361, 522
NspI RCATGY 1 cut(s) 479
PceI AGGCCT 1 cut(s) 206
PcsI WCGNNNNNNNCGW 1 cut(s) 577
PctI GAATGC 1 cut(s) 277
PkrI GCNGC 5 cut(s) 48, 130, 154, 172, 337
PpuMI RGGWCCY 1 cut(s) 52
PsiI TTATAA 1 cut(s) 482
Psp1406I AACGTT 1 cut(s) 459
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 2 cut(s) 724, 793
PspFI CCCAGC 2 cut(s) 389, 835
PspGI CCWGG 2 cut(s) 724, 793
PspN4I GGNNCC 1 cut(s) 396
PspPI GGNCC 2 cut(s) 52, 119
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 1 cut(s) 51
RsaI GTAC 3 cut(s) 5, 266, 325
RsaNI GTAC 3 cut(s) 4, 265, 324
SacII CCGCGG 1 cut(s) 61
SaqAI TTAA 3 cut(s) 533, 764, 825
SatI GCNGC 5 cut(s) 47, 129, 153, 171, 336
Sau3AI GATC 3 cut(s) 224, 543, 863
Sau96I GGNCC 2 cut(s) 52, 119
ScrFI CCNGG 2 cut(s) 726, 795
SfaNI GCATC 1 cut(s) 223
SfcI CTRYAG 1 cut(s) 47
Sfr303I CCGCGG 1 cut(s) 61
SgrBI CCGCGG 1 cut(s) 61
SinI GGWCC 1 cut(s) 52
Sse9I AATT 4 cut(s) 539, 658, 739, 803
SseBI AGGCCT 1 cut(s) 206
SsiI CCGC 6 cut(s) 7, 58, 60, 129, 171, 200
StuI AGGCCT 1 cut(s) 206
StyD4I CCNGG 2 cut(s) 724, 793
StyI CCWWGG 3 cut(s) 165, 219, 237
TaaI ACNGT 2 cut(s) 361, 581
TaiI ACGT 1 cut(s) 462
TaqI TCGA 3 cut(s) 507, 586, 700
TasI AATT 4 cut(s) 539, 658, 739, 803
TatI WGTACW 1 cut(s) 264
TauI GCSGC 2 cut(s) 131, 173
Tru1I TTAA 3 cut(s) 533, 764, 825
Tru9I TTAA 3 cut(s) 533, 764, 825
TseFI GTSAC 2 cut(s) 361, 522
TseI GCWGC 3 cut(s) 46, 152, 335
Tsp45I GTSAC 2 cut(s) 361, 522
TspDTI ATGAA 2 cut(s) 6, 63
TspGWI ACGGA 2 cut(s) 225, 536
VpaK11BI GGWCC 1 cut(s) 52
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 2 cut(s) 658, 803
XceI RCATGY 1 cut(s) 479
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.