RCHIOBHM_MTG0498391

Ribosomal protein S12/S23

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
Mt
OG0010742
Physical Location & Seq
Forward (+)
69959 .. 72527
2569 bp
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UTR
Exon/CDS
Intron
PRQ15611

Sequence Viewer

Length: 489 bp
ATGCCCACATTAAATCAATTGATTCGTCATGGTAGAGAAGAAAAACGGCGCACGGACCGTCCTCGAGCTTCGGATCAATGCCCCCAGAAGCAAGGAGTATGCCCGCGTGTTTCAACGAGAACACCGAAAAACCCTAATTCAGCTCCACGTAATATAGCCAAAGTACGGTTGAGCAATCGACACGATATATTTGCTCAAATTCCAGGCGAAGGTAATAATTTACAGGAACATTCTATGGTGTTAATAAGAGGAGGTAGGGTGAAAGATTTGCCAGGTGTGAAATCCCATTGTATTCGAGGAGTCAAGGATTTGCTGGGATCGAAGAATAGGCGGATCAAAATATGGTGCGGAAAAACCCAAATCGAGATGAATGGAAGATGCCTCTGGAACTTACTTGTTTTTCTAGATCAGTCGAGGAGATTCATAAGCGGATTGCCAAAGAAGGTGCAGGAGCCCCGGATGATTGGGAATCGGAATGTGGGTACTTGA

Protein Analysis

162

Amino Acids

18.48

Weight (kDa)

11.31

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 20 - 106 7.2e-28 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 106
AciI CCGC 4 cut(s) 104, 331, 348, 429
AclWI GGATC 3 cut(s) 81, 325, 341
AcsI RAATTY 1 cut(s) 198
AfaI GTAC 2 cut(s) 165, 484
AfiI CCNNNNNNNGG 2 cut(s) 165, 209
AgsI TTSAA 1 cut(s) 114
AjnI CCWGG 2 cut(s) 202, 271
AluBI AGCT 2 cut(s) 68, 143
AluI AGCT 2 cut(s) 68, 143
AlwI GGATC 3 cut(s) 81, 325, 341
Ama87I CYCGRG 1 cut(s) 63
ApoI RAATTY 1 cut(s) 198
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 1 cut(s) 55
AsuC2I CCSGG 1 cut(s) 457
AsuHPI GGTGA 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 1 cut(s) 55
BanII GRGCYC 1 cut(s) 456
BceAI ACGGC 1 cut(s) 62
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 2 cut(s) 204, 273
BcnI CCSGG 1 cut(s) 457
BfaI CTAG 1 cut(s) 404
Bme1390I CCNGG 3 cut(s) 204, 273, 457
Bme18I GGWCC 1 cut(s) 55
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 1 cut(s) 55
BmiI GGNNCC 1 cut(s) 453
BmrFI CCNGG 3 cut(s) 204, 273, 457
BmsI GCATC 1 cut(s) 368
BpuMI CCSGG 1 cut(s) 457
BsaAI YACGTR 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 455
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 209
BseBI CCWGG 2 cut(s) 204, 273
BseDI CCNNGG 1 cut(s) 455
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 2 cut(s) 165, 209
BseRI GAGGAG 3 cut(s) 264, 312, 430
BseYI CCCAGC 1 cut(s) 313
BsgI GTGCAG 1 cut(s) 467
Bsh1236I CGCG 1 cut(s) 106
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 1 cut(s) 457
BslI CCNNNNNNNGG 2 cut(s) 165, 209
BsoBI CYCGRG 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 456
Bsp143I GATC 4 cut(s) 73, 317, 333, 406
BspACI CCGC 4 cut(s) 104, 331, 348, 429
BspFNI CGCG 1 cut(s) 106
BspLI GGNNCC 1 cut(s) 453
BspPI GGATC 3 cut(s) 81, 325, 341
BssECI CCNNGG 1 cut(s) 455
BssMI GATC 4 cut(s) 73, 317, 333, 406
Bst2UI CCWGG 2 cut(s) 204, 273
Bst4CI ACNGT 2 cut(s) 59, 168
BstBAI YACGTR 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 104
BstF5I GGATG 1 cut(s) 465
BstFNI CGCG 1 cut(s) 106
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 4 cut(s) 76, 320, 336, 409
BstMBI GATC 4 cut(s) 73, 317, 333, 406
BstNI CCWGG 2 cut(s) 204, 273
BstSCI CCNGG 3 cut(s) 202, 271, 455
BstUI CGCG 1 cut(s) 106
BtsCI GGATG 1 cut(s) 465
Cac8I GCNNGC 1 cut(s) 104
CfoI GCGC 1 cut(s) 51
Cfr13I GGNCC 1 cut(s) 55
CpoI CGGWCCG 1 cut(s) 55
Csp6I GTAC 2 cut(s) 164, 483
CspI CGGWCCG 1 cut(s) 55
CviAII CATG 1 cut(s) 29
CviJI RGCY 4 cut(s) 68, 143, 158, 454
CviKI_1 RGCY 4 cut(s) 68, 143, 158, 454
CviQI GTAC 2 cut(s) 164, 483
DpnI GATC 4 cut(s) 75, 319, 335, 408
DpnII GATC 4 cut(s) 73, 317, 333, 406
EciI GGCGGA 1 cut(s) 346
Eco24I GRGCYC 1 cut(s) 456
Eco47I GGWCC 1 cut(s) 55
Eco88I CYCGRG 1 cut(s) 63
EcoRII CCWGG 2 cut(s) 202, 271
EcoT38I GRGCYC 1 cut(s) 456
FaeI CATG 1 cut(s) 32
FaiI YATR 7 cut(s) 30, 100, 155, 188, 236, 343, 425
FatI CATG 1 cut(s) 28
FauI CCCGC 1 cut(s) 111
FokI GGATG 1 cut(s) 472
FriOI GRGCYC 1 cut(s) 456
FspBI CTAG 1 cut(s) 404
GlaI GCGC 1 cut(s) 50
GsaI CCCAGC 1 cut(s) 317
HapII CCGG 1 cut(s) 457
HhaI GCGC 1 cut(s) 51
Hin1II CATG 1 cut(s) 32
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HinfI GANTC 4 cut(s) 22, 300, 420, 469
HpaII CCGG 1 cut(s) 457
HphI GGTGA 1 cut(s) 271
Hpy188I TCNGA 2 cut(s) 73, 474
Hpy188III TCNNGA 3 cut(s) 364, 385, 404
HpyAV CCTTC 2 cut(s) 203, 436
HpyCH4III ACNGT 2 cut(s) 59, 168
HpyCH4IV ACGT 1 cut(s) 148
HpyCH4V TGCA 1 cut(s) 448
HpySE526I ACGT 1 cut(s) 148
Hsp92II CATG 1 cut(s) 32
HspAI GCGC 1 cut(s) 49
Kzo9I GATC 4 cut(s) 73, 317, 333, 406
LmnI GCTCC 2 cut(s) 148, 451
LweI GCATC 1 cut(s) 368
MaeI CTAG 1 cut(s) 404
MaeII ACGT 1 cut(s) 148
MalI GATC 4 cut(s) 75, 319, 335, 408
MboI GATC 4 cut(s) 73, 317, 333, 406
MboII GAAGA 3 cut(s) 50, 334, 387
MfeI CAATTG 1 cut(s) 17
MhlI GDGCHC 1 cut(s) 456
MluCI AATT 4 cut(s) 17, 136, 198, 217
MlyI GAGTC 1 cut(s) 309
MnlI CCTC 6 cut(s) 72, 242, 245, 290, 392, 408
MseI TTAA 2 cut(s) 11, 242
MspI CCGG 1 cut(s) 457
MspR9I CCNGG 3 cut(s) 204, 273, 457
MunI CAATTG 1 cut(s) 17
MvaI CCWGG 2 cut(s) 204, 273
MvnI CGCG 1 cut(s) 106
NciI CCSGG 1 cut(s) 457
NdeII GATC 4 cut(s) 73, 317, 333, 406
NlaIII CATG 1 cut(s) 32
NlaIV GGNNCC 1 cut(s) 453
PaeR7I CTCGAG 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PfeI GAWTC 3 cut(s) 22, 420, 469
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 149
Psp6I CCWGG 2 cut(s) 202, 271
PspFI CCCAGC 1 cut(s) 313
PspGI CCWGG 2 cut(s) 202, 271
PspN4I GGNNCC 1 cut(s) 453
PspPI GGNCC 1 cut(s) 55
PspXI VCTCGAGB 1 cut(s) 63
RsaI GTAC 2 cut(s) 165, 484
RsaNI GTAC 2 cut(s) 164, 483
Rsr2I CGGWCCG 1 cut(s) 55
RsrII CGGWCCG 1 cut(s) 55
SaqAI TTAA 2 cut(s) 11, 242
Sau3AI GATC 4 cut(s) 73, 317, 333, 406
Sau96I GGNCC 1 cut(s) 55
SchI GAGTC 1 cut(s) 309
ScrFI CCNGG 3 cut(s) 204, 273, 457
SduI GDGCHC 1 cut(s) 456
SetI ASST 7 cut(s) 70, 145, 151, 214, 256, 277, 447
SfaNI GCATC 1 cut(s) 368
Sfr274I CTCGAG 1 cut(s) 63
SinI GGWCC 1 cut(s) 55
SlaI CTCGAG 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 63
SmoI CTYRAG 1 cut(s) 63
Sse9I AATT 4 cut(s) 17, 136, 198, 217
SsiI CCGC 4 cut(s) 104, 331, 348, 429
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 3 cut(s) 202, 271, 455
TaaI ACNGT 2 cut(s) 59, 168
TaiI ACGT 1 cut(s) 151
TaqI TCGA 6 cut(s) 64, 178, 295, 320, 363, 413
TasI AATT 4 cut(s) 17, 136, 198, 217
TfiI GAWTC 3 cut(s) 22, 420, 469
Tru1I TTAA 2 cut(s) 11, 242
Tru9I TTAA 2 cut(s) 11, 242
TspDTI ATGAA 2 cut(s) 383, 412
TspGWI ACGGA 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 1 cut(s) 198
XbaI TCTAGA 1 cut(s) 403
XhoI CTCGAG 1 cut(s) 63
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.