Rw5G049230

Ribosomal protein S12/S23

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0010742
Physical Location & Seq
Forward (+)
85312942 .. 85316096
3155 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049230.1

Sequence Viewer

Length: 897 bp
ATGTACCGCATAGCTTCATCCTTCGCCTCTTCAACTCCTAAGAAGCTGCTGGTCCTCGGCGGTAGTAGGGTTTCATCCAATAGGAACTATGTGGCGCAAGGTCTCACCTTCCACATTGGGCCTCGCGCCGCCTTGTTTGAGGTTGCCGACGCTGCCAAGTTCACCATGGCCGCTCTCCTGCAACGCGTCTCTCACCTCGCGGAGGCCTACGGAGTTACCATGGGATCAAAGGATGCCAAGGGCATTGGTTGTTATGAGGACAAGTACAAGAATGCGAGCGAAACGCTTGCGAAACAGGCTACTAATACTGCTGAAGGAGATGGTACAACTTCTGCAGCTGTCCTGACCCAGGCAACAGTCACAGAATGTTGCCAGTCCATAGGTGCTGGGGTTCCCTCAATTTTTGCAAATGGTCAACGAGAGATTGGAGAGTTCTTAGCAAGGGCATTGGAGAACGTTGAAAAGAAAGGACAGGTTATAAGTGAAGATAAAAGTTTCAGTATCGACAGACTGCTCATGTCACCCACATTAAATCAATTGATTCGTCATGGTAGAGAAGAAAAACGGCGCACGGACCGTCCTCGAGCTTCGGATCAATGCCCCCAGAAGCAAGGAGTATGCCCGCGTGTTTCAACGAGAACACCGAAAAACCCTAATTCAGCTCCACGTAATATAGCCAAAGTACGGTTGAGCAATCGACACGATATATTTGCTCAAATTCCAGGCGAAGGTAATAATTTACAGGAACATTCTATGGTGTTAATAAGAGGAGGTAGGGTGAAAGATTTGCCAGGTGTGAAATCCCATTGTATTCGAGGAGTCAAGGATTTGCTGGGACTTCCAAACCGAAGGAGAGGCCGATCAAAATATGGTGCAGAGAAGCCCAAGAGTGCCTGA

Protein Analysis

298

Amino Acids

32.36

Weight (kDa)

9.99

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 193 - 296 3.1e-29 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 479
AccBSI CCGCTC 1 cut(s) 173
AccII CGCG 4 cut(s) 126, 186, 200, 625
AciI CCGC 6 cut(s) 7, 60, 129, 171, 200, 623
AclI AACGTT 1 cut(s) 456
AclWI GGATC 2 cut(s) 232, 600
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 1 cut(s) 717
AcuI CTGAAG 1 cut(s) 333
AfaI GTAC 4 cut(s) 5, 266, 325, 684
AfiI CCNNNNNNNGG 4 cut(s) 202, 349, 684, 728
AflIII ACRYGT 1 cut(s) 184
AgsI TTSAA 3 cut(s) 33, 461, 633
AjnI CCWGG 3 cut(s) 348, 721, 790
AluBI AGCT 5 cut(s) 14, 46, 338, 587, 662
AluI AGCT 5 cut(s) 14, 46, 338, 587, 662
Alw26I GTCTC 2 cut(s) 107, 193
AlwI GGATC 2 cut(s) 232, 600
Ama87I CYCGRG 1 cut(s) 582
AoxI GGCC 4 cut(s) 119, 168, 204, 856
ApeKI GCWGC 3 cut(s) 46, 152, 335
ApoI RAATTY 1 cut(s) 717
AspLEI GCGC 3 cut(s) 97, 128, 570
AspS9I GGNCC 3 cut(s) 52, 119, 574
AsuHPI GGTGA 5 cut(s) 97, 154, 185, 513, 790
AvaI CYCGRG 1 cut(s) 582
AvaII GGWCC 2 cut(s) 52, 574
BbvI GCAGC 3 cut(s) 33, 139, 347
BccI CCATC 1 cut(s) 314
BceAI ACGGC 1 cut(s) 581
BcgI CGANNNNNNTGC 4 cut(s) 269, 303, 692, 726
BciT130I CCWGG 3 cut(s) 350, 723, 792
BcoDI GTCTC 2 cut(s) 107, 193
BfmI CTRYAG 1 cut(s) 333
BisI GCNGC 5 cut(s) 47, 129, 153, 171, 336
BlsI GCNGC 5 cut(s) 48, 130, 154, 172, 337
Bme1390I CCNGG 3 cut(s) 350, 723, 792
Bme18I GGWCC 2 cut(s) 52, 574
BmeT110I CYCGRG 1 cut(s) 582
BmgT120I GGNCC 3 cut(s) 52, 119, 574
BmiI GGNNCC 1 cut(s) 393
BmrFI CCNGG 3 cut(s) 350, 723, 792
BmsI GCATC 1 cut(s) 223
BsaAI YACGTR 1 cut(s) 668
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 5 cut(s) 55, 165, 219, 237, 348
BsaXI ACNNNNNCTCC 2 cut(s) 762, 792
Bsc4I CCNNNNNNNGG 4 cut(s) 202, 349, 684, 728
Bse1I ACTGG 1 cut(s) 373
BseBI CCWGG 3 cut(s) 350, 723, 792
BseDI CCNNGG 5 cut(s) 55, 165, 219, 237, 348
BseGI GGATG 3 cut(s) 17, 74, 238
BseLI CCNNNNNNNGG 4 cut(s) 202, 349, 684, 728
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 2 cut(s) 783, 831
BseXI GCAGC 3 cut(s) 33, 139, 347
BseYI CCCAGC 2 cut(s) 386, 832
BsgI GTGCAG 1 cut(s) 894
Bsh1236I CGCG 4 cut(s) 126, 186, 200, 625
BshFI GGCC 4 cut(s) 121, 170, 206, 858
BsiHKCI CYCGRG 1 cut(s) 582
BslFI GGGAC 1 cut(s) 849
BslI CCNNNNNNNGG 4 cut(s) 202, 349, 684, 728
BsmAI GTCTC 2 cut(s) 107, 193
BsmBI CGTCTC 1 cut(s) 193
BsmFI GGGAC 1 cut(s) 849
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 4 cut(s) 121, 170, 206, 858
Bso31I GGTCTC 1 cut(s) 107
BsoBI CYCGRG 1 cut(s) 582
Bsp143I GATC 3 cut(s) 224, 592, 860
Bsp19I CCATGG 2 cut(s) 165, 219
BspACI CCGC 6 cut(s) 7, 60, 129, 171, 200, 623
BspANI GGCC 4 cut(s) 121, 170, 206, 858
BspFNI CGCG 4 cut(s) 126, 186, 200, 625
BspLI GGNNCC 1 cut(s) 393
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 2 cut(s) 232, 600
BspTNI GGTCTC 1 cut(s) 107
BsrBI CCGCTC 1 cut(s) 173
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 5 cut(s) 55, 165, 219, 237, 348
BssMI GATC 3 cut(s) 224, 592, 860
BssT1I CCWWGG 3 cut(s) 165, 219, 237
Bst2UI CCWGG 3 cut(s) 350, 723, 792
Bst4CI ACNGT 3 cut(s) 358, 578, 687
Bst6I CTCTTC 1 cut(s) 34
BstBAI YACGTR 1 cut(s) 668
BstC8I GCNNGC 3 cut(s) 277, 288, 623
BstDEI CTNAG 2 cut(s) 39, 436
BstDSI CCRYGG 2 cut(s) 165, 219
BstENI CCTNNNNNAGG 2 cut(s) 200, 347
BstF5I GGATG 3 cut(s) 17, 74, 238
BstFNI CGCG 4 cut(s) 126, 186, 200, 625
BstHHI GCGC 3 cut(s) 97, 128, 570
BstKTI GATC 3 cut(s) 227, 595, 863
BstMAI GTCTC 2 cut(s) 107, 193
BstMBI GATC 3 cut(s) 224, 592, 860
BstMWI GCNNNNNNNGC 2 cut(s) 152, 296
BstNI CCWGG 3 cut(s) 350, 723, 792
BstSCI CCNGG 3 cut(s) 348, 721, 790
BstSFI CTRYAG 1 cut(s) 333
BstUI CGCG 4 cut(s) 126, 186, 200, 625
BstV1I GCAGC 3 cut(s) 33, 139, 347
BsuRI GGCC 4 cut(s) 121, 170, 206, 858
BtgI CCRYGG 2 cut(s) 165, 219
BtsCI GGATG 3 cut(s) 17, 74, 238
Cac8I GCNNGC 3 cut(s) 277, 288, 623
CfoI GCGC 3 cut(s) 97, 128, 570
Cfr13I GGNCC 3 cut(s) 52, 119, 574
CpoI CGGWCCG 1 cut(s) 574
CseI GACGC 2 cut(s) 158, 175
Csp6I GTAC 4 cut(s) 4, 265, 324, 683
CspI CGGWCCG 1 cut(s) 574
CviAII CATG 4 cut(s) 166, 220, 517, 548
CviQI GTAC 4 cut(s) 4, 265, 324, 683
DdeI CTNAG 2 cut(s) 39, 436
DpnI GATC 3 cut(s) 226, 594, 862
DpnII GATC 3 cut(s) 224, 592, 860
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
Eco130I CCWWGG 3 cut(s) 165, 219, 237
Eco147I AGGCCT 1 cut(s) 206
Eco31I GGTCTC 1 cut(s) 107
Eco47I GGWCC 2 cut(s) 52, 574
Eco57I CTGAAG 1 cut(s) 333
Eco88I CYCGRG 1 cut(s) 582
EcoNI CCTNNNNNAGG 2 cut(s) 200, 347
EcoRII CCWGG 3 cut(s) 348, 721, 790
EcoT14I CCWWGG 3 cut(s) 165, 219, 237
ErhI CCWWGG 3 cut(s) 165, 219, 237
Esp3I CGTCTC 1 cut(s) 193
FaeI CATG 4 cut(s) 169, 223, 520, 551
FaqI GGGAC 1 cut(s) 849
FatI CATG 4 cut(s) 165, 219, 516, 547
FauI CCCGC 1 cut(s) 630
Fnu4HI GCNGC 5 cut(s) 47, 129, 153, 171, 336
FokI GGATG 3 cut(s) 4, 61, 245
Fsp4HI GCNGC 5 cut(s) 47, 129, 153, 171, 336
GlaI GCGC 3 cut(s) 96, 127, 569
GluI GCNGC 5 cut(s) 47, 129, 153, 171, 336
GsaI CCCAGC 2 cut(s) 390, 836
HaeIII GGCC 4 cut(s) 121, 170, 206, 858
HgaI GACGC 2 cut(s) 158, 175
HhaI GCGC 3 cut(s) 97, 128, 570
Hin1II CATG 4 cut(s) 169, 223, 520, 551
Hin6I GCGC 3 cut(s) 95, 126, 568
HinP1I GCGC 3 cut(s) 95, 126, 568
HincII GTYRAC 1 cut(s) 416
HindII GTYRAC 1 cut(s) 416
HinfI GANTC 2 cut(s) 541, 819
HphI GGTGA 5 cut(s) 97, 154, 185, 513, 790
Hpy166II GTNNAC 2 cut(s) 162, 416
Hpy188I TCNGA 1 cut(s) 592
Hpy188III TCNNGA 1 cut(s) 343
Hpy8I GTNNAC 2 cut(s) 162, 416
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 5 cut(s) 31, 118, 308, 722, 843
HpyCH4III ACNGT 3 cut(s) 358, 578, 687
HpyCH4IV ACGT 2 cut(s) 456, 667
HpyCH4V TGCA 4 cut(s) 181, 335, 407, 875
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 296
HpyF3I CTNAG 2 cut(s) 39, 436
HpySE526I ACGT 2 cut(s) 456, 667
Hsp92II CATG 4 cut(s) 169, 223, 520, 551
HspAI GCGC 3 cut(s) 95, 126, 568
Kzo9I GATC 3 cut(s) 224, 592, 860
LmnI GCTCC 1 cut(s) 667
Lsp1109I GCAGC 3 cut(s) 33, 139, 347
LweI GCATC 1 cut(s) 223
MaeII ACGT 2 cut(s) 456, 667
MaeIII GTNAC 3 cut(s) 214, 358, 519
MalI GATC 3 cut(s) 226, 594, 862
MbiI CCGCTC 1 cut(s) 173
MboI GATC 3 cut(s) 224, 592, 860
MboII GAAGA 3 cut(s) 21, 497, 569
MfeI CAATTG 1 cut(s) 536
MluCI AATT 5 cut(s) 399, 536, 655, 717, 736
MluI ACGCGT 1 cut(s) 184
MlyI GAGTC 1 cut(s) 828
MseI TTAA 2 cut(s) 530, 761
MspA1I CMGCKG 1 cut(s) 338
MspR9I CCNGG 3 cut(s) 350, 723, 792
MunI CAATTG 1 cut(s) 536
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 3 cut(s) 350, 723, 792
MvnI CGCG 4 cut(s) 126, 186, 200, 625
MwoI GCNNNNNNNGC 2 cut(s) 152, 296
NcoI CCATGG 2 cut(s) 165, 219
NdeII GATC 3 cut(s) 224, 592, 860
NlaIII CATG 4 cut(s) 169, 223, 520, 551
NlaIV GGNNCC 1 cut(s) 393
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 2 cut(s) 358, 519
PaeR7I CTCGAG 1 cut(s) 582
PceI AGGCCT 1 cut(s) 206
PcsI WCGNNNNNNNCGW 1 cut(s) 641
PctI GAATGC 1 cut(s) 277
PfeI GAWTC 1 cut(s) 541
PkrI GCNGC 5 cut(s) 48, 130, 154, 172, 337
PleI GAGTC 1 cut(s) 827
PpsI GAGTC 1 cut(s) 827
Ppu21I YACGTR 1 cut(s) 668
PsiI TTATAA 1 cut(s) 479
Psp1406I AACGTT 1 cut(s) 456
Psp6I CCWGG 3 cut(s) 348, 721, 790
PspFI CCCAGC 2 cut(s) 386, 832
PspGI CCWGG 3 cut(s) 348, 721, 790
PspN4I GGNNCC 1 cut(s) 393
PspPI GGNCC 3 cut(s) 52, 119, 574
PspXI VCTCGAGB 1 cut(s) 582
PstI CTGCAG 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 4 cut(s) 5, 266, 325, 684
RsaNI GTAC 4 cut(s) 4, 265, 324, 683
Rsr2I CGGWCCG 1 cut(s) 574
RsrII CGGWCCG 1 cut(s) 574
SaqAI TTAA 2 cut(s) 530, 761
SatI GCNGC 5 cut(s) 47, 129, 153, 171, 336
Sau3AI GATC 3 cut(s) 224, 592, 860
Sau96I GGNCC 3 cut(s) 52, 119, 574
SchI GAGTC 1 cut(s) 828
ScrFI CCNGG 3 cut(s) 350, 723, 792
SfaNI GCATC 1 cut(s) 223
SfcI CTRYAG 1 cut(s) 333
Sfr274I CTCGAG 1 cut(s) 582
SinI GGWCC 2 cut(s) 52, 574
SlaI CTCGAG 1 cut(s) 582
SmlI CTYRAG 1 cut(s) 582
SmoI CTYRAG 1 cut(s) 582
Sse9I AATT 5 cut(s) 399, 536, 655, 717, 736
SseBI AGGCCT 1 cut(s) 206
SsiI CCGC 6 cut(s) 7, 60, 129, 171, 200, 623
StuI AGGCCT 1 cut(s) 206
StyD4I CCNGG 3 cut(s) 348, 721, 790
StyI CCWWGG 3 cut(s) 165, 219, 237
TaaI ACNGT 3 cut(s) 358, 578, 687
TaiI ACGT 2 cut(s) 459, 670
TaqI TCGA 4 cut(s) 504, 583, 697, 814
TasI AATT 5 cut(s) 399, 536, 655, 717, 736
TatI WGTACW 1 cut(s) 264
TauI GCSGC 2 cut(s) 131, 173
TfiI GAWTC 1 cut(s) 541
Tru1I TTAA 2 cut(s) 530, 761
Tru9I TTAA 2 cut(s) 530, 761
TseFI GTSAC 2 cut(s) 358, 519
TseI GCWGC 3 cut(s) 46, 152, 335
Tsp45I GTSAC 2 cut(s) 358, 519
TspDTI ATGAA 2 cut(s) 6, 63
TspGWI ACGGA 2 cut(s) 225, 587
VpaK11BI GGWCC 2 cut(s) 52, 574
XagI CCTNNNNNAGG 2 cut(s) 200, 347
XapI RAATTY 1 cut(s) 717
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
XhoI CTCGAG 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.