Rorug05G0471500

Ribosomal protein S12/S23

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010742
Physical Location & Seq
Forward (+)
64779071 .. 64780420
1350 bp
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UTR
Exon/CDS
Intron
Rorug05G0471500.1

Sequence Viewer

Length: 303 bp
ATGGGTATAGCTGATTCCTTTGTGGAAACAGCCAAGATTGAATTTTTCTATGATCAAGCACCAGAAGGCATGAAAAGCTTGGGGACTTCATATTTCACTACTAGCTTGGGAATTGGGAGTTTCCTCGGTAGTTTTATTCTATCAACAGTTTCCAACTTCACCAAGAAACATACACACAAGGATTGGATTTTGGATAACCGGCCTAAACATCCCTCGTTTAGACTACTATTATCTGTTTTTGGCTGTCTTGACCTTTCTCAACTTCCTCTTCTTTCTCTTGGTTGCGAAATACTATGTCTATAA

Protein Analysis

100

Amino Acids

11.09

Weight (kDa)

6.24

Isoelectric Point (pI)

40.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 1 - 63 1.9e-08 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 41
AgsI TTSAA 1 cut(s) 41
AluBI AGCT 3 cut(s) 11, 78, 105
AluI AGCT 3 cut(s) 11, 78, 105
AoxI GGCC 1 cut(s) 200
ApoI RAATTY 1 cut(s) 41
AsuHPI GGTGA 1 cut(s) 151
BclI TGATCA 1 cut(s) 52
BfaI CTAG 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 124
Bse118I RCCGGY 1 cut(s) 198
BseDI CCNNGG 1 cut(s) 124
BseGI GGATG 1 cut(s) 208
BshFI GGCC 1 cut(s) 202
BsiSI CCGG 1 cut(s) 199
BslFI GGGAC 1 cut(s) 97
BsmFI GGGAC 1 cut(s) 97
BsnI GGCC 1 cut(s) 202
Bsp143I GATC 1 cut(s) 52
BspANI GGCC 1 cut(s) 202
BsrFI RCCGGY 1 cut(s) 198
BssAI RCCGGY 1 cut(s) 198
BssECI CCNNGG 1 cut(s) 124
BssMI GATC 1 cut(s) 52
Bst4CI ACNGT 1 cut(s) 148
Bst6I CTCTTC 1 cut(s) 273
BstF5I GGATG 1 cut(s) 208
BstKTI GATC 1 cut(s) 55
BstMBI GATC 1 cut(s) 52
BstMWI GCNNNNNNNGC 1 cut(s) 75
BsuRI GGCC 1 cut(s) 202
BtsCI GGATG 1 cut(s) 208
Cfr10I RCCGGY 1 cut(s) 198
CviAII CATG 1 cut(s) 70
CviJI RGCY 6 cut(s) 11, 32, 78, 105, 202, 243
CviKI_1 RGCY 6 cut(s) 11, 32, 78, 105, 202, 243
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
FaeI CATG 1 cut(s) 73
FaiI YATR 7 cut(s) 8, 51, 71, 91, 171, 295, 301
FaqI GGGAC 1 cut(s) 97
FatI CATG 1 cut(s) 69
FbaI TGATCA 1 cut(s) 52
FokI GGATG 1 cut(s) 195
FspBI CTAG 1 cut(s) 102
HaeIII GGCC 1 cut(s) 202
HapII CCGG 1 cut(s) 199
Hin1II CATG 1 cut(s) 73
HindIII AAGCTT 1 cut(s) 76
HinfI GANTC 1 cut(s) 14
HpaII CCGG 1 cut(s) 199
HphI GGTGA 1 cut(s) 151
Hpy188III TCNNGA 1 cut(s) 248
HpyAV CCTTC 1 cut(s) 59
HpyCH4III ACNGT 1 cut(s) 148
HpyF10VI GCNNNNNNNGC 1 cut(s) 75
Hsp92II CATG 1 cut(s) 73
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 1 cut(s) 52
LpnPI CCDG 2 cut(s) 75, 212
MaeI CTAG 1 cut(s) 102
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 1 cut(s) 260
MluCI AATT 2 cut(s) 41, 111
MmeI TCCRAC 1 cut(s) 177
MnlI CCTC 3 cut(s) 134, 223, 276
MspI CCGG 1 cut(s) 199
MwoI GCNNNNNNNGC 1 cut(s) 75
NdeII GATC 1 cut(s) 52
NlaIII CATG 1 cut(s) 73
PfeI GAWTC 1 cut(s) 14
Sau3AI GATC 1 cut(s) 52
SetI ASST 4 cut(s) 13, 80, 107, 255
Sse9I AATT 2 cut(s) 41, 111
SspMI CTAG 1 cut(s) 102
TaaI ACNGT 1 cut(s) 148
TasI AATT 2 cut(s) 41, 111
TfiI GAWTC 1 cut(s) 14
TspDTI ATGAA 2 cut(s) 78, 86
XapI RAATTY 1 cut(s) 41
XspI CTAG 1 cut(s) 102
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.