RLG00000000075

Ribosomal protein S12/S23

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Unknown
OG0010742
Physical Location & Seq
Reverse (-)
418269 .. 418763
495 bp
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UTR
Exon/CDS
Intron
RLM00000000075

Sequence Viewer

Length: 495 bp
ATGCCCACATTAAATCAATTGATTCGTCATGGTAGAGAAGAAAAACGGCGCACGGACCGTCCTCGAGCTTCGGATCAATGCCCCCAGAAGCAAGGAGTATGCCCGCGTGTTTCAACGAGAACACCGAAAAACCCTAATTCAGCTCCACGTAATATAGCCAAAGTACGGTTGAGCAATCGACACGATATATTTGCTCAAATTCCAGGCGAAGGTAATAATTTACAGGAACATTCTATGGTGTTAATAAGAGGAGGTAGGGTGAAAGATTTGCCAGGTGTGAAATCCCATTGTATTCGAGGAGTCAAGGATTTGCTGGGATCGAAGAATAGGCGGATCAAAATATGGTGCGGAAAAACCCAAATCGAGATGAATGGAAGATGCCTCTGGAACTTACTTGTTTTTCTAGATCAGTCGAGGTATTCATTGAAAAGTCTCAATGCTATCTTCGACGGTCTTGAGAGCGAGGGCCGCAATAGCGACCACTTTAAACTCTGA

Protein Analysis

165

Amino Acids

18.72

Weight (kDa)

10.46

Isoelectric Point (pI)

42.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 20 - 106 7.4e-28 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 106
AciI CCGC 4 cut(s) 104, 331, 348, 469
AclWI GGATC 3 cut(s) 81, 325, 341
AcsI RAATTY 1 cut(s) 198
AfaI GTAC 1 cut(s) 165
AfiI CCNNNNNNNGG 2 cut(s) 165, 209
AgsI TTSAA 2 cut(s) 114, 427
AjnI CCWGG 2 cut(s) 202, 271
AluBI AGCT 2 cut(s) 68, 143
AluI AGCT 2 cut(s) 68, 143
Alw26I GTCTC 1 cut(s) 437
AlwI GGATC 3 cut(s) 81, 325, 341
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 1 cut(s) 466
ApoI RAATTY 1 cut(s) 198
AspLEI GCGC 1 cut(s) 51
AspS9I GGNCC 2 cut(s) 55, 466
AsuHPI GGTGA 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 1 cut(s) 55
BceAI ACGGC 1 cut(s) 62
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 2 cut(s) 204, 273
BcoDI GTCTC 1 cut(s) 437
BfaI CTAG 1 cut(s) 404
BisI GCNGC 1 cut(s) 469
BlsI GCNGC 1 cut(s) 470
Bme1390I CCNGG 2 cut(s) 204, 273
Bme18I GGWCC 1 cut(s) 55
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 2 cut(s) 55, 466
BmrFI CCNGG 2 cut(s) 204, 273
BmsI GCATC 1 cut(s) 368
BpuEI CTTGAG 1 cut(s) 476
BsaAI YACGTR 1 cut(s) 149
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 209
BseBI CCWGG 2 cut(s) 204, 273
BseLI CCNNNNNNNGG 2 cut(s) 165, 209
BseRI GAGGAG 2 cut(s) 264, 312
BseYI CCCAGC 1 cut(s) 313
Bsh1236I CGCG 1 cut(s) 106
BshFI GGCC 1 cut(s) 468
BsiHKCI CYCGRG 1 cut(s) 63
BslI CCNNNNNNNGG 2 cut(s) 165, 209
BsmAI GTCTC 1 cut(s) 437
BsnI GGCC 1 cut(s) 468
BsoBI CYCGRG 1 cut(s) 63
Bsp143I GATC 4 cut(s) 73, 317, 333, 406
BspACI CCGC 4 cut(s) 104, 331, 348, 469
BspANI GGCC 1 cut(s) 468
BspFNI CGCG 1 cut(s) 106
BspPI GGATC 3 cut(s) 81, 325, 341
BssMI GATC 4 cut(s) 73, 317, 333, 406
Bst2UI CCWGG 2 cut(s) 204, 273
Bst4CI ACNGT 3 cut(s) 59, 168, 452
BstBAI YACGTR 1 cut(s) 149
BstC8I GCNNGC 1 cut(s) 104
BstFNI CGCG 1 cut(s) 106
BstHHI GCGC 1 cut(s) 51
BstKTI GATC 4 cut(s) 76, 320, 336, 409
BstMAI GTCTC 1 cut(s) 437
BstMBI GATC 4 cut(s) 73, 317, 333, 406
BstMWI GCNNNNNNNGC 2 cut(s) 468, 474
BstNI CCWGG 2 cut(s) 204, 273
BstSCI CCNGG 2 cut(s) 202, 271
BstUI CGCG 1 cut(s) 106
BsuRI GGCC 1 cut(s) 468
Cac8I GCNNGC 1 cut(s) 104
CfoI GCGC 1 cut(s) 51
Cfr13I GGNCC 2 cut(s) 55, 466
CpoI CGGWCCG 1 cut(s) 55
Csp6I GTAC 1 cut(s) 164
CspI CGGWCCG 1 cut(s) 55
CviAII CATG 1 cut(s) 29
CviJI RGCY 4 cut(s) 68, 143, 158, 468
CviKI_1 RGCY 4 cut(s) 68, 143, 158, 468
CviQI GTAC 1 cut(s) 164
DpnI GATC 4 cut(s) 75, 319, 335, 408
DpnII GATC 4 cut(s) 73, 317, 333, 406
DraI TTTAAA 1 cut(s) 487
EciI GGCGGA 1 cut(s) 346
Eco47I GGWCC 1 cut(s) 55
Eco88I CYCGRG 1 cut(s) 63
EcoRII CCWGG 2 cut(s) 202, 271
FaeI CATG 1 cut(s) 32
FaiI YATR 6 cut(s) 30, 100, 155, 188, 236, 343
FatI CATG 1 cut(s) 28
FauI CCCGC 1 cut(s) 111
Fnu4HI GCNGC 1 cut(s) 469
Fsp4HI GCNGC 1 cut(s) 469
FspBI CTAG 1 cut(s) 404
GlaI GCGC 1 cut(s) 50
GluI GCNGC 1 cut(s) 469
GsaI CCCAGC 1 cut(s) 317
HaeIII GGCC 1 cut(s) 468
HhaI GCGC 1 cut(s) 51
Hin1II CATG 1 cut(s) 32
Hin6I GCGC 1 cut(s) 49
HinP1I GCGC 1 cut(s) 49
HinfI GANTC 2 cut(s) 22, 300
HphI GGTGA 1 cut(s) 271
Hpy188I TCNGA 2 cut(s) 73, 494
Hpy188III TCNNGA 4 cut(s) 364, 385, 404, 455
Hpy99I CGWCG 1 cut(s) 452
HpyAV CCTTC 1 cut(s) 203
HpyCH4III ACNGT 3 cut(s) 59, 168, 452
HpyCH4IV ACGT 1 cut(s) 148
HpyF10VI GCNNNNNNNGC 2 cut(s) 468, 474
HpySE526I ACGT 1 cut(s) 148
Hsp92II CATG 1 cut(s) 32
HspAI GCGC 1 cut(s) 49
Kzo9I GATC 4 cut(s) 73, 317, 333, 406
LmnI GCTCC 1 cut(s) 148
LpnPI CCDG 8 cut(s) 98, 189, 209, 216, 258, 285, 299, 370
LweI GCATC 1 cut(s) 368
MaeI CTAG 1 cut(s) 404
MaeII ACGT 1 cut(s) 148
MalI GATC 4 cut(s) 75, 319, 335, 408
MboI GATC 4 cut(s) 73, 317, 333, 406
MboII GAAGA 4 cut(s) 50, 334, 387, 436
MfeI CAATTG 1 cut(s) 17
MluCI AATT 4 cut(s) 17, 136, 198, 217
MlyI GAGTC 1 cut(s) 309
MnlI CCTC 7 cut(s) 72, 242, 245, 290, 392, 408, 457
MseI TTAA 3 cut(s) 11, 242, 486
MspR9I CCNGG 2 cut(s) 204, 273
MunI CAATTG 1 cut(s) 17
MvaI CCWGG 2 cut(s) 204, 273
MvnI CGCG 1 cut(s) 106
MwoI GCNNNNNNNGC 2 cut(s) 468, 474
NdeII GATC 4 cut(s) 73, 317, 333, 406
NlaIII CATG 1 cut(s) 32
PaeR7I CTCGAG 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PfeI GAWTC 1 cut(s) 22
PkrI GCNGC 1 cut(s) 470
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 149
Psp6I CCWGG 2 cut(s) 202, 271
PspFI CCCAGC 1 cut(s) 313
PspGI CCWGG 2 cut(s) 202, 271
PspPI GGNCC 2 cut(s) 55, 466
PspXI VCTCGAGB 1 cut(s) 63
RsaI GTAC 1 cut(s) 165
RsaNI GTAC 1 cut(s) 164
Rsr2I CGGWCCG 1 cut(s) 55
RsrII CGGWCCG 1 cut(s) 55
SaqAI TTAA 3 cut(s) 11, 242, 486
SatI GCNGC 1 cut(s) 469
Sau3AI GATC 4 cut(s) 73, 317, 333, 406
Sau96I GGNCC 2 cut(s) 55, 466
SchI GAGTC 1 cut(s) 309
ScrFI CCNGG 2 cut(s) 204, 273
SetI ASST 7 cut(s) 70, 145, 151, 214, 256, 277, 419
SfaNI GCATC 1 cut(s) 368
Sfr274I CTCGAG 1 cut(s) 63
SinI GGWCC 1 cut(s) 55
SlaI CTCGAG 1 cut(s) 63
SmlI CTYRAG 2 cut(s) 63, 455
SmoI CTYRAG 2 cut(s) 63, 455
Sse9I AATT 4 cut(s) 17, 136, 198, 217
SsiI CCGC 4 cut(s) 104, 331, 348, 469
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 2 cut(s) 202, 271
TaaI ACNGT 3 cut(s) 59, 168, 452
TaiI ACGT 1 cut(s) 151
TaqI TCGA 7 cut(s) 64, 178, 295, 320, 363, 413, 447
TasI AATT 4 cut(s) 17, 136, 198, 217
TauI GCSGC 1 cut(s) 471
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 3 cut(s) 11, 242, 486
Tru9I TTAA 3 cut(s) 11, 242, 486
TspDTI ATGAA 2 cut(s) 383, 411
TspGWI ACGGA 1 cut(s) 68
VpaK11BI GGWCC 1 cut(s) 55
XapI RAATTY 1 cut(s) 198
XbaI TCTAGA 1 cut(s) 403
XhoI CTCGAG 1 cut(s) 63
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.