RLG00000000129

Ribosomal protein S12/S23

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Unknown
OG0010742
Physical Location & Seq
Forward (+)
633560 .. 634454
895 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000129

Sequence Viewer

Length: 612 bp
ATGCCCACATTAAATCAATTGATTCGTCATGGTAGAGAAGAAAAACGGCGCACGGACCGTCCTCGAGCTTCGGATCAATGCCCCCAGAAGCAAGGAGTATGCCCGCGTGTTTCAACGAGAACACCGAAAAACCCTAATTCAGCTCCACGTAATATAGCCAAAGTACGGTTGAGCAATCGACACGATATATTTGCTCAAATTCCAGGCGAAGGTAATAATTTACAGGAACATTCTATGGTGTTAATAAGAGGAGGTAGGGTGAAAGATTTGCCAGGTGTGAAATCCCATTGTATTCGAGGAGTCAAGGATTTGCTGGGATCGAAGAATAGGCGGATCAAAATATGGTGCGGAAAAACCCAAATCGAGATGAATGGAAGATGCCTCTGGAACTTACTTGTTTTTCTAGATCAGTCGAGGGAGCGCAGGAGGCTAAGGCTCCGTGTTCGGCTCTGCACAACCGCTTATTTAGTTGATATTGTTGGTTACGGCCCGTGGACCTCGATCAAAAGAGGAAGGCCAATGCCAATGCCCATCATACCATTCGGGGATGTCAGTCAGGCCATTCCAGGAGACAGCAAGCGGGCTGGGACCGAAGCTCGCTATGCGGAATGA

Protein Analysis

204

Amino Acids

23.12

Weight (kDa)

10.99

Isoelectric Point (pI)

52.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 20 - 106 1.3e-27 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 106
AciI CCGC 6 cut(s) 104, 331, 348, 459, 580, 605
AclWI GGATC 3 cut(s) 81, 325, 341
AcsI RAATTY 1 cut(s) 198
AfaI GTAC 1 cut(s) 165
AfiI CCNNNNNNNGG 2 cut(s) 165, 209
AgsI TTSAA 1 cut(s) 114
AjnI CCWGG 3 cut(s) 202, 271, 565
AluBI AGCT 3 cut(s) 68, 143, 596
AluI AGCT 3 cut(s) 68, 143, 596
Alw26I GTCTC 1 cut(s) 564
AlwI GGATC 3 cut(s) 81, 325, 341
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 3 cut(s) 487, 515, 558
ApoI RAATTY 1 cut(s) 198
AspLEI GCGC 2 cut(s) 51, 423
AspS9I GGNCC 4 cut(s) 55, 488, 495, 588
AsuHPI GGTGA 1 cut(s) 271
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 3 cut(s) 55, 495, 588
BccI CCATC 1 cut(s) 539
BceAI ACGGC 2 cut(s) 62, 502
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 3 cut(s) 204, 273, 567
BcoDI GTCTC 1 cut(s) 564
BfaI CTAG 1 cut(s) 404
Bme1390I CCNGG 3 cut(s) 204, 273, 567
Bme18I GGWCC 3 cut(s) 55, 495, 588
BmeT110I CYCGRG 1 cut(s) 63
BmgT120I GGNCC 4 cut(s) 55, 488, 495, 588
BmiI GGNNCC 2 cut(s) 437, 589
BmrFI CCNGG 3 cut(s) 204, 273, 567
BmsI GCATC 1 cut(s) 368
Bpu10I CCTNAGC 1 cut(s) 431
BsaAI YACGTR 1 cut(s) 149
BsaJI CCNNGG 1 cut(s) 491
BsaXI ACNNNNNCTCC 2 cut(s) 243, 273
Bsc4I CCNNNNNNNGG 2 cut(s) 165, 209
BseBI CCWGG 3 cut(s) 204, 273, 567
BseDI CCNNGG 1 cut(s) 491
BseGI GGATG 1 cut(s) 553
BseLI CCNNNNNNNGG 2 cut(s) 165, 209
BseRI GAGGAG 2 cut(s) 264, 312
BseYI CCCAGC 2 cut(s) 313, 584
BsgI GTGCAG 1 cut(s) 436
Bsh1236I CGCG 1 cut(s) 106
BshFI GGCC 3 cut(s) 489, 517, 560
BsiHKCI CYCGRG 1 cut(s) 63
BslFI GGGAC 1 cut(s) 601
BslI CCNNNNNNNGG 2 cut(s) 165, 209
BsmAI GTCTC 1 cut(s) 564
BsmFI GGGAC 1 cut(s) 601
BsnI GGCC 3 cut(s) 489, 517, 560
BsoBI CYCGRG 1 cut(s) 63
Bsp143I GATC 5 cut(s) 73, 317, 333, 406, 501
BspACI CCGC 6 cut(s) 104, 331, 348, 459, 580, 605
BspANI GGCC 3 cut(s) 489, 517, 560
BspFNI CGCG 1 cut(s) 106
BspLI GGNNCC 2 cut(s) 437, 589
BspPI GGATC 3 cut(s) 81, 325, 341
BssECI CCNNGG 1 cut(s) 491
BssMI GATC 5 cut(s) 73, 317, 333, 406, 501
Bst2UI CCWGG 3 cut(s) 204, 273, 567
Bst4CI ACNGT 2 cut(s) 59, 168
BstBAI YACGTR 1 cut(s) 149
BstC8I GCNNGC 4 cut(s) 104, 578, 582, 598
BstDEI CTNAG 1 cut(s) 431
BstDSI CCRYGG 1 cut(s) 491
BstF5I GGATG 1 cut(s) 553
BstFNI CGCG 1 cut(s) 106
BstHHI GCGC 2 cut(s) 51, 423
BstKTI GATC 5 cut(s) 76, 320, 336, 409, 504
BstMAI GTCTC 1 cut(s) 564
BstMBI GATC 5 cut(s) 73, 317, 333, 406, 501
BstMWI GCNNNNNNNGC 2 cut(s) 427, 602
BstNI CCWGG 3 cut(s) 204, 273, 567
BstSCI CCNGG 3 cut(s) 202, 271, 565
BstUI CGCG 1 cut(s) 106
BsuRI GGCC 3 cut(s) 489, 517, 560
BtgI CCRYGG 1 cut(s) 491
BtsCI GGATG 1 cut(s) 553
Cac8I GCNNGC 4 cut(s) 104, 578, 582, 598
CfoI GCGC 2 cut(s) 51, 423
Cfr13I GGNCC 4 cut(s) 55, 488, 495, 588
CpoI CGGWCCG 1 cut(s) 55
Csp6I GTAC 1 cut(s) 164
CspI CGGWCCG 1 cut(s) 55
CviAII CATG 1 cut(s) 29
CviQI GTAC 1 cut(s) 164
DdeI CTNAG 1 cut(s) 431
DpnI GATC 5 cut(s) 75, 319, 335, 408, 503
DpnII GATC 5 cut(s) 73, 317, 333, 406, 501
EciI GGCGGA 1 cut(s) 346
Eco47I GGWCC 3 cut(s) 55, 495, 588
Eco88I CYCGRG 1 cut(s) 63
EcoRII CCWGG 3 cut(s) 202, 271, 565
FaeI CATG 1 cut(s) 32
FaiI YATR 8 cut(s) 30, 100, 155, 188, 236, 343, 536, 603
FaqI GGGAC 1 cut(s) 601
FatI CATG 1 cut(s) 28
FauI CCCGC 2 cut(s) 111, 573
FokI GGATG 1 cut(s) 560
FspBI CTAG 1 cut(s) 404
GlaI GCGC 2 cut(s) 50, 422
GsaI CCCAGC 2 cut(s) 317, 588
HaeIII GGCC 3 cut(s) 489, 517, 560
HhaI GCGC 2 cut(s) 51, 423
Hin1II CATG 1 cut(s) 32
Hin6I GCGC 2 cut(s) 49, 421
HinP1I GCGC 2 cut(s) 49, 421
HinfI GANTC 2 cut(s) 22, 300
HphI GGTGA 1 cut(s) 271
Hpy166II GTNNAC 1 cut(s) 495
Hpy188I TCNGA 1 cut(s) 73
Hpy188III TCNNGA 3 cut(s) 364, 385, 404
Hpy8I GTNNAC 1 cut(s) 495
HpyAV CCTTC 2 cut(s) 203, 507
HpyCH4III ACNGT 2 cut(s) 59, 168
HpyCH4IV ACGT 1 cut(s) 148
HpyCH4V TGCA 1 cut(s) 453
HpyF10VI GCNNNNNNNGC 2 cut(s) 427, 602
HpyF3I CTNAG 1 cut(s) 431
HpySE526I ACGT 1 cut(s) 148
Hsp92II CATG 1 cut(s) 32
HspAI GCGC 2 cut(s) 49, 421
Kzo9I GATC 5 cut(s) 73, 317, 333, 406, 501
LmnI GCTCC 3 cut(s) 148, 418, 441
LweI GCATC 1 cut(s) 368
MaeI CTAG 1 cut(s) 404
MaeII ACGT 1 cut(s) 148
MaeIII GTNAC 1 cut(s) 482
MalI GATC 5 cut(s) 75, 319, 335, 408, 503
MboI GATC 5 cut(s) 73, 317, 333, 406, 501
MboII GAAGA 3 cut(s) 50, 334, 387
MfeI CAATTG 1 cut(s) 17
MluCI AATT 4 cut(s) 17, 136, 198, 217
MlyI GAGTC 1 cut(s) 309
MnlI CCTC 9 cut(s) 72, 242, 245, 290, 392, 408, 420, 503, 508
MseI TTAA 2 cut(s) 11, 242
MspR9I CCNGG 3 cut(s) 204, 273, 567
MunI CAATTG 1 cut(s) 17
MvaI CCWGG 3 cut(s) 204, 273, 567
MvnI CGCG 1 cut(s) 106
MwoI GCNNNNNNNGC 2 cut(s) 427, 602
NdeII GATC 5 cut(s) 73, 317, 333, 406, 501
NlaIII CATG 1 cut(s) 32
NlaIV GGNNCC 2 cut(s) 437, 589
PaeR7I CTCGAG 1 cut(s) 63
PcsI WCGNNNNNNNCGW 1 cut(s) 122
PfeI GAWTC 1 cut(s) 22
PfoI TCCNGGA 1 cut(s) 565
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
Ppu21I YACGTR 1 cut(s) 149
Psp6I CCWGG 3 cut(s) 202, 271, 565
PspFI CCCAGC 2 cut(s) 313, 584
PspGI CCWGG 3 cut(s) 202, 271, 565
PspN4I GGNNCC 2 cut(s) 437, 589
PspPI GGNCC 4 cut(s) 55, 488, 495, 588
PspXI VCTCGAGB 1 cut(s) 63
RsaI GTAC 1 cut(s) 165
RsaNI GTAC 1 cut(s) 164
Rsr2I CGGWCCG 1 cut(s) 55
RsrII CGGWCCG 1 cut(s) 55
SaqAI TTAA 2 cut(s) 11, 242
Sau3AI GATC 5 cut(s) 73, 317, 333, 406, 501
Sau96I GGNCC 4 cut(s) 55, 488, 495, 588
SchI GAGTC 1 cut(s) 309
ScrFI CCNGG 3 cut(s) 204, 273, 567
SetI ASST 8 cut(s) 70, 145, 151, 214, 256, 277, 500, 598
SfaNI GCATC 1 cut(s) 368
Sfr274I CTCGAG 1 cut(s) 63
SinI GGWCC 3 cut(s) 55, 495, 588
SlaI CTCGAG 1 cut(s) 63
SmlI CTYRAG 1 cut(s) 63
SmoI CTYRAG 1 cut(s) 63
Sse9I AATT 4 cut(s) 17, 136, 198, 217
SsiI CCGC 6 cut(s) 104, 331, 348, 459, 580, 605
SspMI CTAG 1 cut(s) 404
StyD4I CCNGG 3 cut(s) 202, 271, 565
TaaI ACNGT 2 cut(s) 59, 168
TaiI ACGT 1 cut(s) 151
TaqI TCGA 7 cut(s) 64, 178, 295, 320, 363, 413, 500
TaqII GACCGA 1 cut(s) 605
TasI AATT 4 cut(s) 17, 136, 198, 217
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 2 cut(s) 11, 242
Tru9I TTAA 2 cut(s) 11, 242
TspDTI ATGAA 1 cut(s) 383
TspGWI ACGGA 2 cut(s) 68, 428
VpaK11BI GGWCC 3 cut(s) 55, 495, 588
XapI RAATTY 1 cut(s) 198
XbaI TCTAGA 1 cut(s) 403
XhoI CTCGAG 1 cut(s) 63
XspI CTAG 1 cut(s) 404
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.