Rh5CG570200

Ribosomal protein S12/S23

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0010742
Physical Location & Seq
Forward (+)
80606856 .. 80610538
3683 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG570200.1

Sequence Viewer

Length: 897 bp
ATGTACCGCATAGCTTCATCCTTCGCCTCTTCAACTCCTAAGAAGCTGCTGGTCCTCGGCGGTAGTAGGGTTTCATCCAATAGGAACTATGTGGCGCAAGGTCTCACCTTCCACATTGGGCCTCGCGCCGCCTTGTTTGAGGTTGCCGACGCTGCCAAGTTCACCATGGCCGCTCTGCTGCAACGTGTCTCTCACCTCGCGGAGGCCTACGGAGTTACCATGGGATCAAAGGATGCCAAGGGCATTGGTTGTTATGAGGACAAGTACAAGAATGCGAGCGAAACACTTGCGAAACAGGCTACTAATACTGCCGGAGGAGATGGTACAACTTCTGCAGCTGTCTTGACCCAGGCAACAGTCACAGAATGTTGCCAGTCCATAGGTGCTGGGGTTCCCTCTATTTTTGCAAATGGTCAACGAGAGATTGGAGAGTTCTTAGCAAGGGCATTGGAGAACGTTGAAAAGAAAGGACATGTTATAAGTGAAGATAAAAGTTTCAGTATCGACAGACTGCTCATGTCACCCACATTAAATCAATTGATCCGTCATAATAGAACTGAGAAAAGTCGCACGAACCGTAATCGAGCTTTGGATAAATGCCCCCAAAAGCAAGGAGTATGCCTACGGGTTTTTACGAGAACACCCAAGAAGCCAAATTCAGCTCTGCGTAAGGTAGCCAAAGTAAGGCTAAGCAATCGAAATGATGTATTTTCTTACATTCCAGGTGAAGGTCATAATTTGCAGGAACATTCTATGGTCTTAATAAGAGGGGGCAGAGTGAAAGATTTGCCAGGTGTGAAGTTCCATTGTATCAGAGGAGTTAAGGATTTGCTGGGACTTCCAAACCGAAGGAGAGGCCGATCAAAATATGGCGCAGAGAAGCCCAAGAGTGCCTGA

Protein Analysis

298

Amino Acids

32.5

Weight (kDa)

10.14

Isoelectric Point (pI)

35.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosom_S12_S23 PF00164 192 - 296 7.9e-37 Ribosomal protein S12/S23
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 479
AccBSI CCGCTC 1 cut(s) 173
AccII CGCG 2 cut(s) 126, 200
AciI CCGC 5 cut(s) 7, 60, 129, 171, 200
AclI AACGTT 1 cut(s) 456
AclWI GGATC 2 cut(s) 232, 535
AcoI YGGCCR 1 cut(s) 168
AcsI RAATTY 1 cut(s) 655
AfaI GTAC 3 cut(s) 5, 266, 325
AfiI CCNNNNNNNGG 3 cut(s) 202, 684, 728
AflIII ACRYGT 2 cut(s) 184, 472
AgsI TTSAA 2 cut(s) 33, 461
AjnI CCWGG 3 cut(s) 348, 721, 790
AluBI AGCT 5 cut(s) 14, 46, 338, 587, 662
AluI AGCT 5 cut(s) 14, 46, 338, 587, 662
Alw26I GTCTC 2 cut(s) 107, 193
AlwI GGATC 2 cut(s) 232, 535
AoxI GGCC 4 cut(s) 119, 168, 204, 856
ApeKI GCWGC 4 cut(s) 46, 152, 178, 335
ApoI RAATTY 1 cut(s) 655
AspLEI GCGC 3 cut(s) 97, 128, 875
AspS9I GGNCC 2 cut(s) 52, 119
AsuHPI GGTGA 5 cut(s) 97, 154, 185, 513, 737
AvaII GGWCC 1 cut(s) 52
BbvI GCAGC 4 cut(s) 33, 139, 165, 347
BccI CCATC 1 cut(s) 314
BcgI CGANNNNNNTGC 2 cut(s) 269, 303
BciT130I CCWGG 3 cut(s) 350, 723, 792
BcoDI GTCTC 2 cut(s) 107, 193
BfmI CTRYAG 1 cut(s) 333
BisI GCNGC 6 cut(s) 47, 129, 153, 171, 179, 336
BlpI GCTNAGC 1 cut(s) 689
BlsI GCNGC 6 cut(s) 48, 130, 154, 172, 180, 337
Bme1390I CCNGG 3 cut(s) 350, 723, 792
Bme18I GGWCC 1 cut(s) 52
BmgT120I GGNCC 2 cut(s) 52, 119
BmiI GGNNCC 1 cut(s) 393
BmrFI CCNGG 3 cut(s) 350, 723, 792
BmsI GCATC 1 cut(s) 223
Bpu1102I GCTNAGC 1 cut(s) 689
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 5 cut(s) 55, 165, 219, 237, 348
Bsc4I CCNNNNNNNGG 3 cut(s) 202, 684, 728
Bse1I ACTGG 1 cut(s) 373
BseBI CCWGG 3 cut(s) 350, 723, 792
BseDI CCNNGG 5 cut(s) 55, 165, 219, 237, 348
BseGI GGATG 3 cut(s) 17, 74, 238
BseLI CCNNNNNNNGG 3 cut(s) 202, 684, 728
BseMII CTCAG 1 cut(s) 549
BseNI ACTGG 1 cut(s) 373
BseRI GAGGAG 2 cut(s) 330, 831
BseXI GCAGC 4 cut(s) 33, 139, 165, 347
BseYI CCCAGC 2 cut(s) 386, 832
Bsh1236I CGCG 2 cut(s) 126, 200
BshFI GGCC 4 cut(s) 121, 170, 206, 858
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 1 cut(s) 849
BslI CCNNNNNNNGG 3 cut(s) 202, 684, 728
BsmAI GTCTC 2 cut(s) 107, 193
BsmFI GGGAC 1 cut(s) 849
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 4 cut(s) 121, 170, 206, 858
Bso31I GGTCTC 1 cut(s) 107
Bsp143I GATC 3 cut(s) 224, 540, 860
Bsp1720I GCTNAGC 1 cut(s) 689
Bsp19I CCATGG 2 cut(s) 165, 219
BspACI CCGC 5 cut(s) 7, 60, 129, 171, 200
BspANI GGCC 4 cut(s) 121, 170, 206, 858
BspCNI CTCAG 1 cut(s) 550
BspFNI CGCG 2 cut(s) 126, 200
BspLI GGNNCC 1 cut(s) 393
BspMAI CTGCAG 1 cut(s) 337
BspPI GGATC 2 cut(s) 232, 535
BspTNI GGTCTC 1 cut(s) 107
BsrBI CCGCTC 1 cut(s) 173
BsrI ACTGG 1 cut(s) 373
BssECI CCNNGG 5 cut(s) 55, 165, 219, 237, 348
BssMI GATC 3 cut(s) 224, 540, 860
BssT1I CCWWGG 3 cut(s) 165, 219, 237
Bst2UI CCWGG 3 cut(s) 350, 723, 792
Bst4CI ACNGT 2 cut(s) 358, 578
Bst6I CTCTTC 1 cut(s) 34
BstC8I GCNNGC 1 cut(s) 277
BstDEI CTNAG 4 cut(s) 39, 436, 558, 689
BstDSI CCRYGG 2 cut(s) 165, 219
BstENI CCTNNNNNAGG 1 cut(s) 200
BstF5I GGATG 3 cut(s) 17, 74, 238
BstFNI CGCG 2 cut(s) 126, 200
BstHHI GCGC 3 cut(s) 97, 128, 875
BstKTI GATC 3 cut(s) 227, 543, 863
BstMAI GTCTC 2 cut(s) 107, 193
BstMBI GATC 3 cut(s) 224, 540, 860
BstMWI GCNNNNNNNGC 2 cut(s) 152, 296
BstNI CCWGG 3 cut(s) 350, 723, 792
BstNSI RCATGY 1 cut(s) 476
BstSCI CCNGG 3 cut(s) 348, 721, 790
BstSFI CTRYAG 1 cut(s) 333
BstUI CGCG 2 cut(s) 126, 200
BstV1I GCAGC 4 cut(s) 33, 139, 165, 347
BsuRI GGCC 4 cut(s) 121, 170, 206, 858
BtgI CCRYGG 2 cut(s) 165, 219
BtsCI GGATG 3 cut(s) 17, 74, 238
Cac8I GCNNGC 1 cut(s) 277
CfoI GCGC 3 cut(s) 97, 128, 875
Cfr13I GGNCC 2 cut(s) 52, 119
CseI GACGC 1 cut(s) 158
Csp6I GTAC 3 cut(s) 4, 265, 324
CviAII CATG 4 cut(s) 166, 220, 473, 517
CviQI GTAC 3 cut(s) 4, 265, 324
DdeI CTNAG 4 cut(s) 39, 436, 558, 689
DpnI GATC 3 cut(s) 226, 542, 862
DpnII GATC 3 cut(s) 224, 540, 860
EaeI YGGCCR 1 cut(s) 168
Eam1104I CTCTTC 1 cut(s) 34
EarI CTCTTC 1 cut(s) 34
Eco130I CCWWGG 3 cut(s) 165, 219, 237
Eco147I AGGCCT 1 cut(s) 206
Eco31I GGTCTC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 52
EcoNI CCTNNNNNAGG 1 cut(s) 200
EcoRII CCWGG 3 cut(s) 348, 721, 790
EcoT14I CCWWGG 3 cut(s) 165, 219, 237
ErhI CCWWGG 3 cut(s) 165, 219, 237
FaeI CATG 4 cut(s) 169, 223, 476, 520
FaqI GGGAC 1 cut(s) 849
FatI CATG 4 cut(s) 165, 219, 472, 516
Fnu4HI GCNGC 6 cut(s) 47, 129, 153, 171, 179, 336
FokI GGATG 3 cut(s) 4, 61, 245
Fsp4HI GCNGC 6 cut(s) 47, 129, 153, 171, 179, 336
GlaI GCGC 3 cut(s) 96, 127, 874
GluI GCNGC 6 cut(s) 47, 129, 153, 171, 179, 336
GsaI CCCAGC 2 cut(s) 390, 836
HaeIII GGCC 4 cut(s) 121, 170, 206, 858
HapII CCGG 1 cut(s) 312
HgaI GACGC 1 cut(s) 158
HhaI GCGC 3 cut(s) 97, 128, 875
Hin1II CATG 4 cut(s) 169, 223, 476, 520
Hin6I GCGC 3 cut(s) 95, 126, 873
HinP1I GCGC 3 cut(s) 95, 126, 873
HincII GTYRAC 1 cut(s) 416
HindII GTYRAC 1 cut(s) 416
HpaII CCGG 1 cut(s) 312
HphI GGTGA 5 cut(s) 97, 154, 185, 513, 737
Hpy166II GTNNAC 2 cut(s) 162, 416
Hpy188I TCNGA 1 cut(s) 815
Hpy188III TCNNGA 1 cut(s) 343
Hpy8I GTNNAC 2 cut(s) 162, 416
Hpy99I CGWCG 1 cut(s) 152
HpyAV CCTTC 4 cut(s) 31, 118, 722, 843
HpyCH4III ACNGT 2 cut(s) 358, 578
HpyCH4IV ACGT 2 cut(s) 184, 456
HpyCH4V TGCA 4 cut(s) 181, 335, 407, 742
HpyF10VI GCNNNNNNNGC 2 cut(s) 152, 296
HpyF3I CTNAG 4 cut(s) 39, 436, 558, 689
HpySE526I ACGT 2 cut(s) 184, 456
Hsp92II CATG 4 cut(s) 169, 223, 476, 520
HspAI GCGC 3 cut(s) 95, 126, 873
Kzo9I GATC 3 cut(s) 224, 540, 860
Lsp1109I GCAGC 4 cut(s) 33, 139, 165, 347
LweI GCATC 1 cut(s) 223
MaeII ACGT 2 cut(s) 184, 456
MaeIII GTNAC 3 cut(s) 214, 358, 519
MalI GATC 3 cut(s) 226, 542, 862
MbiI CCGCTC 1 cut(s) 173
MboI GATC 3 cut(s) 224, 540, 860
MboII GAAGA 2 cut(s) 21, 497
MfeI CAATTG 1 cut(s) 536
MluCI AATT 3 cut(s) 536, 655, 736
MseI TTAA 3 cut(s) 530, 761, 822
MspA1I CMGCKG 1 cut(s) 338
MspI CCGG 1 cut(s) 312
MspR9I CCNGG 3 cut(s) 350, 723, 792
MunI CAATTG 1 cut(s) 536
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 3 cut(s) 350, 723, 792
MvnI CGCG 2 cut(s) 126, 200
MwoI GCNNNNNNNGC 2 cut(s) 152, 296
NcoI CCATGG 2 cut(s) 165, 219
NdeII GATC 3 cut(s) 224, 540, 860
NlaIII CATG 4 cut(s) 169, 223, 476, 520
NlaIV GGNNCC 1 cut(s) 393
NmeAIII GCCGAG 1 cut(s) 36
NmuCI GTSAC 2 cut(s) 358, 519
NspI RCATGY 1 cut(s) 476
PceI AGGCCT 1 cut(s) 206
PciI ACATGT 1 cut(s) 472
PcsI WCGNNNNNNNCGW 1 cut(s) 574
PctI GAATGC 1 cut(s) 277
PkrI GCNGC 6 cut(s) 48, 130, 154, 172, 180, 337
PscI ACATGT 1 cut(s) 472
PsiI TTATAA 1 cut(s) 479
Psp1406I AACGTT 1 cut(s) 456
Psp6I CCWGG 3 cut(s) 348, 721, 790
PspFI CCCAGC 2 cut(s) 386, 832
PspGI CCWGG 3 cut(s) 348, 721, 790
PspN4I GGNNCC 1 cut(s) 393
PspPI GGNCC 2 cut(s) 52, 119
PstI CTGCAG 1 cut(s) 337
PvuII CAGCTG 1 cut(s) 338
RsaI GTAC 3 cut(s) 5, 266, 325
RsaNI GTAC 3 cut(s) 4, 265, 324
SaqAI TTAA 3 cut(s) 530, 761, 822
SatI GCNGC 6 cut(s) 47, 129, 153, 171, 179, 336
Sau3AI GATC 3 cut(s) 224, 540, 860
Sau96I GGNCC 2 cut(s) 52, 119
ScrFI CCNGG 3 cut(s) 350, 723, 792
SfaNI GCATC 1 cut(s) 223
SfcI CTRYAG 1 cut(s) 333
SinI GGWCC 1 cut(s) 52
Sse9I AATT 3 cut(s) 536, 655, 736
SseBI AGGCCT 1 cut(s) 206
SsiI CCGC 5 cut(s) 7, 60, 129, 171, 200
StuI AGGCCT 1 cut(s) 206
StyD4I CCNGG 3 cut(s) 348, 721, 790
StyI CCWWGG 3 cut(s) 165, 219, 237
TaaI ACNGT 2 cut(s) 358, 578
TaiI ACGT 2 cut(s) 187, 459
TaqI TCGA 3 cut(s) 504, 583, 697
TasI AATT 3 cut(s) 536, 655, 736
TatI WGTACW 1 cut(s) 264
TauI GCSGC 2 cut(s) 131, 173
Tru1I TTAA 3 cut(s) 530, 761, 822
Tru9I TTAA 3 cut(s) 530, 761, 822
TseFI GTSAC 2 cut(s) 358, 519
TseI GCWGC 4 cut(s) 46, 152, 178, 335
Tsp45I GTSAC 2 cut(s) 358, 519
TspDTI ATGAA 2 cut(s) 6, 63
TspGWI ACGGA 2 cut(s) 225, 533
VpaK11BI GGWCC 1 cut(s) 52
XagI CCTNNNNNAGG 1 cut(s) 200
XapI RAATTY 1 cut(s) 655
XceI RCATGY 1 cut(s) 476
XcmI CCANNNNNNNNNTGG 1 cut(s) 163
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.