RchiOBHm_Chr4g0427061

Autophagy-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0018962
Physical Location & Seq
Reverse (-)
52345769 .. 52347471
1703 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1014 bp
ATGATTGGTGGAAGGCCCTTAGTGCAGAGATGCGGTCCAGAGGAGGAGGAGGAGGAGGAGGGTGGGCTCTTGAGTTCGGGTTGGGCGGATCGGTCTCGATCTGGCCTTCTGGGTTCAGGGGCGGCGACGGCCGAGTTATTGCAGCGGTTTGGTTCTTTCCGGCGGAGGGACTCGGAGTCATCGCCTAGGCGGGCTGCATCATGGTGGCGGCGGTCAAATCGCGGCAGAAGGGTTCCCTGTAGGGAGCTGTTTCTATCGTGCGTTTGGATCTGGGTGGGATTGTCAACAGGATGTAGATTCTGTTTGCAAATTGGCATGGTGGAGTGGCGATGGCAGGCAACGGCGAGGCAGTGGGGTGATGACGATGGTGGTTGCCGGCGGGGGGAATGGTTGTCTGCGGTGATTGGGTGTTGTTGGGGGAAGAGGGCTCTGGGCCGGGCCCCTGTCTTTGGGCTTTGGTGTTTAATAAGTCCCTACTCTTTTGAGCTAGGGTTGGTGTCCTACTCTCTTGAGCTAGGAATTGGTGTAGGTGTTAAGCCATGTAAATGGTGTCATTCCCGAGGACGAGGACGATTTTGTTCAACTCTGGTTTACTTTTGTTGTCGATTTGCTGATAAGCGATCCTTTACACGTGGTCTAGCACCTTCGGACACGGTGTTGAAGAGGACGCTGTCTTCTTCGCAGTTGATTATAAGGTTTTACTGGAATTTTCGGGTTTATCTGCTCAACGTAGCCTTGGAAATAGGCGTTTGGTGGTTAGAGTTTGGGCCCTTTGGCCTAATGGTCTCTGTAACCGCGGTTTCTTGGGGTTTTGATATATACGTGGTTCCTAGAGATATATCTGTTGGCCAGTTTATCCATATTTTAAGCAGCCGGCTTCAACTGACTCCTGGGAAAGCGCTTTTTGTATTTGTGAAGACCTCAAACAGTCGTATGGATTCCATTTACGAAAGTTACAAGGAAGATGATGGATTTCTGTATATGTGTTACAGCAGCGAGAAAACCTTTGGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04068 ko04136 ko04137 ko04138 ko04139 ko04140 ko04212 ko04371 ko04621 ko04727 ko05167 map04068 map04136 map04137 map04138 map04139 map04140 map04212 map04371 map04621 map04727 map05167
Pfam Domains
Protein Families
Atg8

Protein Analysis

337

Amino Acids

38.26

Weight (kDa)

9.21

Isoelectric Point (pI)

59.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ATG8 PF02991 274 - 337 2.3e-20 Autophagy protein Atg8 ubiquitin like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 692
AccII CGCG 2 cut(s) 222, 797
AclWI GGATC 3 cut(s) 96, 275, 615
AcoI YGGCCR 2 cut(s) 129, 847
AcsI RAATTY 1 cut(s) 706
AcvI CACGTG 1 cut(s) 632
AfeI AGCGCT 1 cut(s) 900
AfiI CCNNNNNNNGG 3 cut(s) 166, 382, 449
AflIII ACRYGT 1 cut(s) 629
AgsI TTSAA 3 cut(s) 582, 661, 881
AhdI GACNNNNNGTC 1 cut(s) 175
AjnI CCWGG 1 cut(s) 889
AluBI AGCT 3 cut(s) 247, 487, 514
AluI AGCT 3 cut(s) 247, 487, 514
Alw26I GTCTC 2 cut(s) 99, 790
AlwI GGATC 3 cut(s) 96, 275, 615
Ama87I CYCGRG 1 cut(s) 558
Aor51HI AGCGCT 1 cut(s) 900
AoxI GGCC 8 cut(s) 14, 103, 129, 433, 438, 767, 775, 847
ApaI GGGCCC 2 cut(s) 442, 771
ApeKI GCWGC 4 cut(s) 142, 194, 870, 993
ApoI RAATTY 1 cut(s) 706
AspA2I CCTAGG 1 cut(s) 185
AspLEI GCGC 1 cut(s) 901
AspS9I GGNCC 7 cut(s) 15, 35, 433, 438, 439, 767, 768
AsuC2I CCSGG 1 cut(s) 437
AsuHPI GGTGA 2 cut(s) 368, 412
AvaI CYCGRG 1 cut(s) 558
AvaII GGWCC 1 cut(s) 35
AvrII CCTAGG 1 cut(s) 185
BaeGI GKGCMC 2 cut(s) 442, 771
BalI TGGCCA 1 cut(s) 849
BanII GRGCYC 4 cut(s) 69, 430, 442, 771
BbrPI CACGTG 1 cut(s) 632
BbsI GAAGAC 2 cut(s) 666, 923
BbvI GCAGC 4 cut(s) 154, 181, 882, 1005
BccI CCATC 3 cut(s) 324, 359, 962
BceAI ACGGC 2 cut(s) 144, 357
BciT130I CCWGG 1 cut(s) 891
BcnI CCSGG 1 cut(s) 437
BcoDI GTCTC 2 cut(s) 99, 790
BfaI CTAG 5 cut(s) 186, 488, 515, 638, 831
BfmI CTRYAG 1 cut(s) 238
BfoI RGCGCY 1 cut(s) 902
BisI GCNGC 7 cut(s) 123, 143, 195, 209, 223, 871, 994
BlnI CCTAGG 1 cut(s) 185
BlsI GCNGC 7 cut(s) 124, 144, 196, 210, 224, 872, 995
Bme1390I CCNGG 2 cut(s) 437, 891
Bme18I GGWCC 1 cut(s) 35
BmeRI GACNNNNNGTC 1 cut(s) 175
BmeT110I CYCGRG 1 cut(s) 558
BmgT120I GGNCC 7 cut(s) 15, 35, 433, 438, 439, 767, 768
BmiI GGNNCC 5 cut(s) 234, 440, 441, 769, 828
BmrFI CCNGG 2 cut(s) 437, 891
BmsI GCATC 2 cut(s) 20, 206
BpiI GAAGAC 2 cut(s) 666, 923
BpuEI CTTGAG 2 cut(s) 91, 530
BpuMI CCSGG 1 cut(s) 437
BsaAI YACGTR 2 cut(s) 632, 823
BsaI GGTCTC 2 cut(s) 99, 790
BsaJI CCNNGG 5 cut(s) 185, 559, 735, 795, 890
Bsc4I CCNNNNNNNGG 3 cut(s) 166, 382, 449
Bse118I RCCGGY 2 cut(s) 375, 873
Bse1I ACTGG 2 cut(s) 707, 850
BseBI CCWGG 1 cut(s) 891
BseDI CCNNGG 5 cut(s) 185, 559, 735, 795, 890
BseGI GGATG 1 cut(s) 296
BseLI CCNNNNNNNGG 3 cut(s) 166, 382, 449
BseNI ACTGG 2 cut(s) 707, 850
BseRI GAGGAG 6 cut(s) 56, 59, 62, 65, 68, 71
BseSI GKGCMC 2 cut(s) 442, 771
BseX3I CGGCCG 1 cut(s) 129
BseXI GCAGC 4 cut(s) 154, 181, 882, 1005
BsgI GTGCAG 1 cut(s) 44
Bsh1236I CGCG 2 cut(s) 222, 797
Bsh1285I CGRYCG 1 cut(s) 132
BshFI GGCC 8 cut(s) 16, 105, 131, 435, 440, 769, 777, 849
BsiEI CGRYCG 1 cut(s) 132
BsiHKCI CYCGRG 1 cut(s) 558
BsiSI CCGG 4 cut(s) 160, 376, 436, 874
BslFI GGGAC 2 cut(s) 182, 456
BslI CCNNNNNNNGG 3 cut(s) 166, 382, 449
BsmAI GTCTC 2 cut(s) 99, 790
BsmFI GGGAC 2 cut(s) 182, 456
BsnI GGCC 8 cut(s) 16, 105, 131, 435, 440, 769, 777, 849
Bso31I GGTCTC 2 cut(s) 99, 790
BsoBI CYCGRG 1 cut(s) 558
Bsp120I GGGCCC 2 cut(s) 438, 767
Bsp1286I GDGCHC 4 cut(s) 69, 430, 442, 771
Bsp143I GATC 4 cut(s) 88, 98, 267, 620
BspANI GGCC 8 cut(s) 16, 105, 131, 435, 440, 769, 777, 849
BspFNI CGCG 2 cut(s) 222, 797
BspLI GGNNCC 5 cut(s) 234, 440, 441, 769, 828
BspPI GGATC 3 cut(s) 96, 275, 615
BspTNI GGTCTC 2 cut(s) 99, 790
BsrFI RCCGGY 2 cut(s) 375, 873
BsrI ACTGG 2 cut(s) 707, 850
BssAI RCCGGY 2 cut(s) 375, 873
BssECI CCNNGG 5 cut(s) 185, 559, 735, 795, 890
BssMI GATC 4 cut(s) 88, 98, 267, 620
BssT1I CCWWGG 2 cut(s) 185, 735
Bst2UI CCWGG 1 cut(s) 891
Bst4CI ACNGT 2 cut(s) 655, 929
Bst6I CTCTTC 2 cut(s) 416, 656
BstBAI YACGTR 2 cut(s) 632, 823
BstC8I GCNNGC 4 cut(s) 192, 336, 377, 875
BstDEI CTNAG 1 cut(s) 19
BstDSI CCRYGG 1 cut(s) 795
BstF5I GGATG 1 cut(s) 296
BstFNI CGCG 2 cut(s) 222, 797
BstH2I RGCGCY 1 cut(s) 902
BstHHI GCGC 1 cut(s) 901
BstKTI GATC 4 cut(s) 91, 101, 270, 623
BstMAI GTCTC 2 cut(s) 99, 790
BstMBI GATC 4 cut(s) 88, 98, 267, 620
BstMCI CGRYCG 1 cut(s) 132
BstMWI GCNNNNNNNGC 2 cut(s) 22, 128
BstNI CCWGG 1 cut(s) 891
BstSCI CCNGG 2 cut(s) 435, 889
BstSFI CTRYAG 1 cut(s) 238
BstSLI GKGCMC 2 cut(s) 442, 771
BstUI CGCG 2 cut(s) 222, 797
BstV1I GCAGC 4 cut(s) 154, 181, 882, 1005
BstV2I GAAGAC 2 cut(s) 666, 923
BstX2I RGATCY 1 cut(s) 267
BstXI CCANNNNNNTGG 1 cut(s) 546
BstYI RGATCY 1 cut(s) 267
BstZI CGGCCG 1 cut(s) 129
BsuRI GGCC 8 cut(s) 16, 105, 131, 435, 440, 769, 777, 849
BtgI CCRYGG 1 cut(s) 795
BtgZI GCGATG 2 cut(s) 165, 343
BtsCI GGATG 1 cut(s) 296
BtsI GCAGTG 1 cut(s) 356
BtsIMutI CAGTG 1 cut(s) 356
Cac8I GCNNGC 4 cut(s) 192, 336, 377, 875
CfoI GCGC 1 cut(s) 901
Cfr10I RCCGGY 2 cut(s) 375, 873
Cfr13I GGNCC 7 cut(s) 15, 35, 433, 438, 439, 767, 768
Cfr42I CCGCGG 1 cut(s) 798
CseI GACGC 1 cut(s) 676
CviAII CATG 3 cut(s) 201, 316, 540
DdeI CTNAG 1 cut(s) 19
DpnI GATC 4 cut(s) 90, 100, 269, 622
DpnII GATC 4 cut(s) 88, 98, 267, 620
DriI GACNNNNNGTC 1 cut(s) 175
EaeI YGGCCR 2 cut(s) 129, 847
EagI CGGCCG 1 cut(s) 129
Eam1104I CTCTTC 2 cut(s) 416, 656
Eam1105I GACNNNNNGTC 1 cut(s) 175
EarI CTCTTC 2 cut(s) 416, 656
EciI GGCGGA 2 cut(s) 101, 178
EclXI CGGCCG 1 cut(s) 129
Eco130I CCWWGG 2 cut(s) 185, 735
Eco24I GRGCYC 4 cut(s) 69, 430, 442, 771
Eco31I GGTCTC 2 cut(s) 99, 790
Eco47I GGWCC 1 cut(s) 35
Eco47III AGCGCT 1 cut(s) 900
Eco52I CGGCCG 1 cut(s) 129
Eco72I CACGTG 1 cut(s) 632
Eco88I CYCGRG 1 cut(s) 558
EcoO109I RGGNCCY 3 cut(s) 15, 439, 768
EcoRII CCWGG 1 cut(s) 889
EcoT14I CCWWGG 2 cut(s) 185, 735
EcoT38I GRGCYC 4 cut(s) 69, 430, 442, 771
ErhI CCWWGG 2 cut(s) 185, 735
FaeI CATG 3 cut(s) 204, 319, 543
FalI AAGNNNNNCTT 2 cut(s) 608, 640
FaqI GGGAC 2 cut(s) 182, 456
FatI CATG 3 cut(s) 200, 315, 539
FauI CCCGC 2 cut(s) 183, 372
Fnu4HI GCNGC 7 cut(s) 123, 143, 195, 209, 223, 871, 994
FokI GGATG 1 cut(s) 303
FriOI GRGCYC 4 cut(s) 69, 430, 442, 771
Fsp4HI GCNGC 7 cut(s) 123, 143, 195, 209, 223, 871, 994
FspBI CTAG 5 cut(s) 186, 488, 515, 638, 831
GlaI GCGC 1 cut(s) 900
GluI GCNGC 7 cut(s) 123, 143, 195, 209, 223, 871, 994
HaeII RGCGCY 1 cut(s) 902
HaeIII GGCC 8 cut(s) 16, 105, 131, 435, 440, 769, 777, 849
HapII CCGG 4 cut(s) 160, 376, 436, 874
HgaI GACGC 1 cut(s) 676
HhaI GCGC 1 cut(s) 901
Hin1II CATG 3 cut(s) 204, 319, 543
Hin6I GCGC 1 cut(s) 899
HinP1I GCGC 1 cut(s) 899
HincII GTYRAC 1 cut(s) 285
HindII GTYRAC 1 cut(s) 285
HinfI GANTC 5 cut(s) 170, 176, 297, 886, 938
HpaII CCGG 4 cut(s) 160, 376, 436, 874
HphI GGTGA 2 cut(s) 368, 412
Hpy166II GTNNAC 2 cut(s) 285, 592
Hpy188I TCNGA 2 cut(s) 175, 649
Hpy188III TCNNGA 5 cut(s) 38, 70, 96, 509, 558
Hpy8I GTNNAC 2 cut(s) 285, 592
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 4 cut(s) 6, 116, 222, 654
HpyCH4III ACNGT 2 cut(s) 655, 929
HpyCH4IV ACGT 3 cut(s) 631, 729, 822
HpyCH4V TGCA 4 cut(s) 25, 142, 197, 307
HpyF10VI GCNNNNNNNGC 2 cut(s) 22, 128
HpyF3I CTNAG 1 cut(s) 19
HpySE526I ACGT 3 cut(s) 631, 729, 822
Hsp92II CATG 3 cut(s) 204, 319, 543
HspAI GCGC 1 cut(s) 899
KroI GCCGGC 2 cut(s) 375, 873
KroNI GCCGGC 2 cut(s) 377, 875
KspI CCGCGG 1 cut(s) 798
Kzo9I GATC 4 cut(s) 88, 98, 267, 620
LmnI GCTCC 1 cut(s) 244
Lsp1109I GCAGC 4 cut(s) 154, 181, 882, 1005
LweI GCATC 2 cut(s) 20, 206
MaeI CTAG 5 cut(s) 186, 488, 515, 638, 831
MaeII ACGT 3 cut(s) 631, 729, 822
MaeIII GTNAC 3 cut(s) 790, 953, 986
MalI GATC 4 cut(s) 90, 100, 269, 622
MboI GATC 4 cut(s) 88, 98, 267, 620
MboII GAAGA 6 cut(s) 433, 666, 669, 673, 928, 974
MflI RGATCY 1 cut(s) 267
MhlI GDGCHC 4 cut(s) 69, 430, 442, 771
MlsI TGGCCA 1 cut(s) 849
MluCI AATT 3 cut(s) 309, 519, 706
MluNI TGGCCA 1 cut(s) 849
MlyI GAGTC 3 cut(s) 164, 185, 880
Mox20I TGGCCA 1 cut(s) 849
MroNI GCCGGC 2 cut(s) 375, 873
MscI TGGCCA 1 cut(s) 849
MseI TTAA 3 cut(s) 464, 534, 866
MslI CAYNNNNRTG 2 cut(s) 202, 544
Msp20I TGGCCA 1 cut(s) 849
MspA1I CMGCKG 2 cut(s) 145, 797
MspI CCGG 4 cut(s) 160, 376, 436, 874
MspR9I CCNGG 2 cut(s) 437, 891
MvaI CCWGG 1 cut(s) 891
MvnI CGCG 2 cut(s) 222, 797
MwoI GCNNNNNNNGC 2 cut(s) 22, 128
NaeI GCCGGC 2 cut(s) 377, 875
NciI CCSGG 1 cut(s) 437
NdeII GATC 4 cut(s) 88, 98, 267, 620
NgoMIV GCCGGC 2 cut(s) 375, 873
NlaIII CATG 3 cut(s) 204, 319, 543
NlaIV GGNNCC 5 cut(s) 234, 440, 441, 769, 828
NmeAIII GCCGAG 1 cut(s) 157
PdiI GCCGGC 2 cut(s) 377, 875
PfeI GAWTC 2 cut(s) 297, 938
PflFI GACNNNGTC 1 cut(s) 670
PkrI GCNGC 7 cut(s) 124, 144, 196, 210, 224, 872, 995
PleI GAGTC 3 cut(s) 164, 184, 880
PmaCI CACGTG 1 cut(s) 632
PmlI CACGTG 1 cut(s) 632
PpsI GAGTC 3 cut(s) 164, 184, 880
Ppu21I YACGTR 2 cut(s) 632, 823
PsiI TTATAA 1 cut(s) 692
Psp6I CCWGG 1 cut(s) 889
PspCI CACGTG 1 cut(s) 632
PspGI CCWGG 1 cut(s) 889
PspN4I GGNNCC 5 cut(s) 234, 440, 441, 769, 828
PspOMI GGGCCC 2 cut(s) 438, 767
PspPI GGNCC 7 cut(s) 15, 35, 433, 438, 439, 767, 768
PsuI RGATCY 1 cut(s) 267
PsyI GACNNNGTC 1 cut(s) 670
RseI CAYNNNNRTG 2 cut(s) 202, 544
SacII CCGCGG 1 cut(s) 798
SaqAI TTAA 3 cut(s) 464, 534, 866
SatI GCNGC 7 cut(s) 123, 143, 195, 209, 223, 871, 994
Sau3AI GATC 4 cut(s) 88, 98, 267, 620
Sau96I GGNCC 7 cut(s) 15, 35, 433, 438, 439, 767, 768
SchI GAGTC 3 cut(s) 164, 185, 880
ScrFI CCNGG 2 cut(s) 437, 891
SduI GDGCHC 4 cut(s) 69, 430, 442, 771
SfaNI GCATC 2 cut(s) 20, 206
SfcI CTRYAG 1 cut(s) 238
Sfr303I CCGCGG 1 cut(s) 798
SgrBI CCGCGG 1 cut(s) 798
SinI GGWCC 1 cut(s) 35
SmiMI CAYNNNNRTG 2 cut(s) 202, 544
SmlI CTYRAG 2 cut(s) 70, 509
SmoI CTYRAG 2 cut(s) 70, 509
Sse9I AATT 3 cut(s) 309, 519, 706
SspMI CTAG 5 cut(s) 186, 488, 515, 638, 831
StyD4I CCNGG 2 cut(s) 435, 889
StyI CCWWGG 2 cut(s) 185, 735
TaaI ACNGT 2 cut(s) 655, 929
TaiI ACGT 3 cut(s) 634, 732, 825
TaqI TCGA 2 cut(s) 97, 604
TaqII GACCGA 1 cut(s) 81
TasI AATT 3 cut(s) 309, 519, 706
TauI GCSGC 3 cut(s) 125, 211, 225
TfiI GAWTC 2 cut(s) 297, 938
Tru1I TTAA 3 cut(s) 464, 534, 866
Tru9I TTAA 3 cut(s) 464, 534, 866
TscAI CASTG 1 cut(s) 356
TseI GCWGC 4 cut(s) 142, 194, 870, 993
TspRI CASTG 1 cut(s) 356
Tth111I GACNNNGTC 1 cut(s) 670
VpaK11BI GGWCC 1 cut(s) 35
XapI RAATTY 1 cut(s) 706
XmaJI CCTAGG 1 cut(s) 185
XspI CTAG 5 cut(s) 186, 488, 515, 638, 831
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.