RchiOBHm_Chr3g0454961

Belongs to the peptidase M24B family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0011079
Physical Location & Seq
Reverse (-)
4831054 .. 4836212
5159 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2115 bp
ATGCACTCCCTCTCATCCCATGCGACGCGTCCTCTCTCCTCTTCCTTCTCTCACTCCACCTCCTACTTCCGCTTCATCTCCCTCTCCCTCCCATTTCTCCACTCTCCCAAATCCCACCCCAAACTTTTCACTATTTCCCCAAAAGCCCCCACTTTCACGATCCGGAGCATCTCCGCCAAGCCCTCGTCGGAGCTCCGACCCAAGCGCCCCAATTCCGACTCCGACTCAAAGCTCCGAGCTCTCCGCGACCTCTTCTCCAAGCCCGGCGTCGGAATCGACGCTTACATCATTCCCTCTCAGGATGCTCACCAGAGTGAGTTCATTGCTGAATGTTATATGAGGAGGACCTATATATCTGGTTTCACAGGGAGTGCCGGCACTGCTGTTGTCACTAAGGATAAAGCAGCTCTTTGGACAGATGGCCGCTATTTTCTTCAGGCTGAAAAGCAGCTGAGTTCGAGTTGGATTCTCATGCGGGCTGGTAATTTTGGAGTACCTACAACTATTGAATGGCTGAATGATGTTTTGGCCCCTGGTGGGAGAGTTGGCATTGACCCTTTTCTGTTTTCTTCCGATGCTGCAGAAGAATTGAAGCAGGGCATCGCTAACAAGAATCATGAGTTGGTTTTTCTGTATGATAAAAATCTTGTGGATGAAATATGGAAAGAATCAAGACCAGAGCCTCCAAAAAAACCAATTAGAGTACACGAACTGAAATATGCTGGTGCAGATGTGGCATCAAAGTTATCATCTTTGAGGTCTGAACTTGTTGCAGCTGGTTCATCTACGATAGTTATATCTATGCTTGATGAAGTTGCCTGGCTGTTGAATTTGAGAGGAAGTGATGTTCCACATTCACCTGTTATGTATGCTTATCTGATTGTGGAGATCGACAAAGCAAAGTTATTTGTAGATACTTCCAAAGTAGGCACTGAGGTGTTGGATCACCTGGAGAATGCGGGTATTGAATTGAGGCCATATGACTCCATTCTTTCTGAAGTAGAAAGTCTGGCAGCAAAAGGGGCTAAAGTTTGGTTGGATGCGTCATCTGTGAATGCAGCTATTGTCCACACCTACAACTCTGCTTGTGAGAAATATTACGAGAGCCTTGAAAGTAAAAGAAAAAGTAAGACTAAGGAATATGATGACTCAAATGGTTGGTCTGAAGGACCCACTGGAATGTATAGGGTGTCCCCTGTCTCTTTGGCAAAGGCACTGAAAAATGATGCTGAGTTAGAAGGGATGCGGAGTTGTCATTTAAGGGATGCTGCTGCTTTAGCGCAGTTTTGGGTTTGGATCGAAGAGCAAATCAACAAGAATGTGAAACTAACTGAGGTAGAAGCTGCAGAAAAACTTCTTGAATTTCGTTCAAGGCAGCCTGGTTTCCTAGATACAAGCTTTGACACCATCAGTGGCTCTGGTGCAAATGGTGCTATCATACATTATAAACCAGAACCAGATAGTTGCAGTGTTGTGAATGAAAGGAAACTCTTTCTGTTGGATAGTGGAGCTCAGTATGTGGACGGAACAACTGACATAACAAGGACCGTACATTTTGGTGAACCTTCTCCACGTCAAAAAGACTGCTTCACCCGAGTTTTACAGGGTCATATAGCTCTTGATCAGGCCGTATTCCCTGAAAACACACCGGGTTTTGTCTTAGATGCATTCGCTCGTTCTTTTCTTTGGAAGATTGGACTGGATTACCGACATGGAACTGGGCACGGTGTGGGAGCTGCATTAAATGTTCATGAGGGTCCTCAAAGTATTAGCTTTCGATTTGGAAATACAACTTCCTTACAGAGTGGTATGATTGTTAGCAATGAACCTGGCTATTATGAAGACCATGCCTTCGGCATTCGGATTGAGAATCTCCTTGTTGTGAAAGAAGTTGACACGCCAAATCGTTTTGGAGGAATTGGATACCTGGGATTTGAAAAACTCACATATGTTCCCATCCAGGCTAAATTGATAGACTTGTCTTTGCTTTCTGGTGCGGAATTTGATTGGCTTAATGATTATCATTCACAAGTTTGGGACAAGGTCTCACCATTGCTCGATGGTCCTGCACGCCAATGGCTTTGGAACAACACAAGACCACTTGTCAAGCAGTGA

Protein Analysis

704

Amino Acids

78.18

Weight (kDa)

6.16

Isoelectric Point (pI)

47.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Creatinase_N PF01321 80 - 204 7.9e-14 Creatinase/Prolidase N-terminal domain
Creatinase_N_2 PF16189 225 - 411 8.3e-46 Creatinase/Prolidase N-terminal domain
Peptidase_M24 PF00557 413 - 630 2.1e-51 Metallopeptidase family M24
Peptidase_M24_C PF16188 641 - 701 6.1e-25 C-terminal region of peptidase_M24
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1446
AccII CGCG 2 cut(s) 28, 246
AccIII TCCGGA 1 cut(s) 162
AciI CCGC 8 cut(s) 70, 174, 244, 424, 475, 959, 1246, 1997
AclWI GGATC 3 cut(s) 154, 951, 1304
AcoI YGGCCR 1 cut(s) 421
AcsI RAATTY 3 cut(s) 829, 1361, 2000
AcuI CTGAAG 3 cut(s) 419, 1017, 1185
AcyI GRCGYC 1 cut(s) 267
AdeI CACNNNGTG 1 cut(s) 1729
AfaI GTAC 3 cut(s) 495, 705, 1551
AfiI CCNNNNNNNGG 2 cut(s) 269, 537
AflIII ACRYGT 1 cut(s) 26
AgsI TTSAA 8 cut(s) 509, 592, 829, 968, 1112, 1361, 1371, 1937
AhdI GACNNNNNGTC 1 cut(s) 2102
AjiI CACGTC 1 cut(s) 1574
AjnI CCWGG 7 cut(s) 532, 818, 948, 1378, 1828, 1926, 1959
AjuI GAANNNNNNNTTGG 4 cut(s) 509, 541, 605, 637
AleI CACNNNNGTG 2 cut(s) 312, 935
AloI GAACNNNNNNTCC 2 cut(s) 831, 863
Alw21I GWGCWC 3 cut(s) 195, 241, 1513
Alw26I GTCTC 2 cut(s) 1204, 2050
AlwI GGATC 3 cut(s) 154, 951, 1304
Ama87I CYCGRG 1 cut(s) 1593
Aor13HI TCCGGA 1 cut(s) 162
AoxI GGCC 4 cut(s) 421, 528, 974, 1626
ApoI RAATTY 3 cut(s) 829, 1361, 2000
ArsI GACNNNNNNTTYG 4 cut(s) 1145, 1177, 1835, 1867
Asp700I GAANNNNTTC 1 cut(s) 1353
AspLEI GCGC 2 cut(s) 207, 1282
AspS9I GGNCC 6 cut(s) 345, 529, 1169, 1545, 1757, 2063
AsuC2I CCSGG 2 cut(s) 264, 1650
AsuHPI GGTGA 6 cut(s) 299, 849, 938, 1571, 1582, 2040
AvaI CYCGRG 1 cut(s) 1593
AvaII GGWCC 5 cut(s) 345, 1169, 1545, 1757, 2063
BaeGI GKGCMC 1 cut(s) 1725
BanII GRGCYC 3 cut(s) 195, 241, 1513
BbsI GAAGAC 1 cut(s) 1848
Bbv12I GWGCWC 3 cut(s) 195, 241, 1513
BccI CCATC 4 cut(s) 413, 1415, 1964, 2054
BceAI ACGGC 1 cut(s) 1613
BcgI CGANNNNNNTGC 2 cut(s) 2048, 2082
BciT130I CCWGG 7 cut(s) 534, 820, 950, 1380, 1830, 1928, 1961
BciVI GTATCC 1 cut(s) 1916
BclI TGATCA 1 cut(s) 1621
BcnI CCSGG 2 cut(s) 264, 1650
BcoDI GTCTC 2 cut(s) 1204, 2050
BfaI CTAG 1 cut(s) 1388
BfmI CTRYAG 2 cut(s) 579, 1344
BfoI RGCGCY 1 cut(s) 208
BfuI GTATCC 1 cut(s) 1916
Bme1390I CCNGG 9 cut(s) 264, 534, 820, 950, 1380, 1650, 1830, 1928, 1961
Bme18I GGWCC 5 cut(s) 345, 1169, 1545, 1757, 2063
BmeRI GACNNNNNGTC 1 cut(s) 2102
BmeT110I CYCGRG 1 cut(s) 1593
BmgBI CACGTC 1 cut(s) 1574
BmgT120I GGNCC 6 cut(s) 345, 529, 1169, 1545, 1757, 2063
BmiI GGNNCC 3 cut(s) 531, 1171, 1758
BmrFI CCNGG 9 cut(s) 264, 534, 820, 950, 1380, 1650, 1830, 1928, 1961
BmrI ACTGGG 1 cut(s) 1728
BmuI ACTGGG 1 cut(s) 1728
BpiI GAAGAC 1 cut(s) 1848
BpmI CTGGAG 1 cut(s) 971
BpuMI CCSGG 2 cut(s) 264, 1650
BsaBI GATNNNNATC 1 cut(s) 642
BsaHI GRCGYC 1 cut(s) 267
BsaI GGTCTC 1 cut(s) 2050
BsaJI CCNNGG 2 cut(s) 532, 1927
BsaWI WCCGGW 1 cut(s) 162
BsaXI ACNNNNNCTCC 4 cut(s) 44, 74, 239, 269
Bsc4I CCNNNNNNNGG 2 cut(s) 269, 537
Bse118I RCCGGY 1 cut(s) 374
Bse1I ACTGG 3 cut(s) 1180, 1704, 1723
Bse3DI GCAATG 3 cut(s) 321, 1828, 2051
Bse8I GATNNNNATC 1 cut(s) 642
BseAI TCCGGA 1 cut(s) 162
BseBI CCWGG 7 cut(s) 534, 820, 950, 1380, 1830, 1928, 1961
BseDI CCNNGG 2 cut(s) 532, 1927
BseGI GGATG 7 cut(s) 14, 307, 658, 1045, 1248, 1270, 1956
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 2 cut(s) 269, 537
BseMI GCAATG 3 cut(s) 321, 1828, 2051
BseMII CTCAG 6 cut(s) 311, 443, 924, 1221, 1323, 1526
BseNI ACTGG 3 cut(s) 1180, 1704, 1723
BseRI GAGGAG 2 cut(s) 28, 355
BseSI GKGCMC 1 cut(s) 1725
BsgI GTGCAG 2 cut(s) 747, 2052
Bsh1236I CGCG 2 cut(s) 28, 246
BshFI GGCC 4 cut(s) 423, 530, 976, 1628
BsiHKAI GWGCWC 3 cut(s) 195, 241, 1513
BsiHKCI CYCGRG 1 cut(s) 1593
BsiSI CCGG 4 cut(s) 163, 264, 375, 1649
BslFI GGGAC 2 cut(s) 1177, 2051
BslI CCNNNNNNNGG 2 cut(s) 269, 537
BsmAI GTCTC 2 cut(s) 1204, 2050
BsmFI GGGAC 2 cut(s) 1177, 2051
BsmI GAATGC 4 cut(s) 961, 1060, 1667, 1857
BsnI GGCC 4 cut(s) 423, 530, 976, 1628
Bso31I GGTCTC 1 cut(s) 2050
BsoBI CYCGRG 1 cut(s) 1593
Bsp1286I GDGCHC 4 cut(s) 195, 241, 1513, 1725
Bsp13I TCCGGA 1 cut(s) 162
Bsp143I GATC 5 cut(s) 159, 888, 943, 1296, 1621
BspACI CCGC 8 cut(s) 70, 174, 244, 424, 475, 959, 1246, 1997
BspANI GGCC 4 cut(s) 423, 530, 976, 1628
BspCNI CTCAG 6 cut(s) 310, 444, 925, 1222, 1324, 1525
BspEI TCCGGA 1 cut(s) 162
BspFNI CGCG 2 cut(s) 28, 246
BspHI TCATGA 2 cut(s) 616, 1750
BspLI GGNNCC 3 cut(s) 531, 1171, 1758
BspMAI CTGCAG 2 cut(s) 583, 1348
BspPI GGATC 3 cut(s) 154, 951, 1304
BspQI GCTCTTC 1 cut(s) 1296
BspTNI GGTCTC 1 cut(s) 2050
BsrDI GCAATG 3 cut(s) 321, 1828, 2051
BsrFI RCCGGY 1 cut(s) 374
BsrI ACTGG 3 cut(s) 1180, 1704, 1723
BssAI RCCGGY 1 cut(s) 374
BssECI CCNNGG 2 cut(s) 532, 1927
BssMI GATC 5 cut(s) 159, 888, 943, 1296, 1621
BssNI GRCGYC 1 cut(s) 267
Bst2UI CCWGG 7 cut(s) 534, 820, 950, 1380, 1830, 1928, 1961
Bst4CI ACNGT 2 cut(s) 1549, 1727
Bst6I CTCTTC 3 cut(s) 46, 257, 1296
BstACI GRCGYC 1 cut(s) 267
BstAPI GCANNNNNTGC 1 cut(s) 1430
BstC8I GCNNGC 3 cut(s) 376, 477, 2071
BstDEI CTNAG 9 cut(s) 297, 393, 452, 933, 1134, 1230, 1332, 1512, 1660
BstF5I GGATG 7 cut(s) 14, 307, 658, 1045, 1248, 1270, 1956
BstFNI CGCG 2 cut(s) 28, 246
BstH2I RGCGCY 1 cut(s) 208
BstHHI GCGC 2 cut(s) 207, 1282
BstKTI GATC 5 cut(s) 162, 891, 946, 1299, 1624
BstMAI GTCTC 2 cut(s) 1204, 2050
BstMBI GATC 5 cut(s) 159, 888, 943, 1296, 1621
BstMWI GCNNNNNNNGC 5 cut(s) 380, 734, 1022, 1277, 1430
BstNI CCWGG 7 cut(s) 534, 820, 950, 1380, 1830, 1928, 1961
BstSCI CCNGG 9 cut(s) 262, 532, 818, 948, 1378, 1648, 1828, 1926, 1959
BstSFI CTRYAG 2 cut(s) 579, 1344
BstSLI GKGCMC 1 cut(s) 1725
BstUI CGCG 2 cut(s) 28, 246
BstV2I GAAGAC 1 cut(s) 1848
BsuI GTATCC 1 cut(s) 1916
BsuRI GGCC 4 cut(s) 423, 530, 976, 1628
BtgZI GCGATG 1 cut(s) 586
BtrI CACGTC 1 cut(s) 1574
BtsCI GGATG 7 cut(s) 14, 307, 658, 1045, 1248, 1270, 1956
BtsI GCAGTG 2 cut(s) 378, 1474
BtsIMutI CAGTG 6 cut(s) 378, 930, 1173, 1214, 1417, 1474
Cac8I GCNNGC 3 cut(s) 376, 477, 2071
CciI TCATGA 2 cut(s) 616, 1750
CfoI GCGC 2 cut(s) 207, 1282
Cfr10I RCCGGY 1 cut(s) 374
Cfr13I GGNCC 6 cut(s) 345, 529, 1169, 1545, 1757, 2063
CseI GACGC 5 cut(s) 17, 34, 256, 287, 1032
Csp6I GTAC 3 cut(s) 494, 704, 1550
CviAII CATG 6 cut(s) 20, 472, 617, 1712, 1751, 1847
CviQI GTAC 3 cut(s) 494, 704, 1550
DdeI CTNAG 9 cut(s) 297, 393, 452, 933, 1134, 1230, 1332, 1512, 1660
DpnI GATC 5 cut(s) 161, 890, 945, 1298, 1623
DpnII GATC 5 cut(s) 159, 888, 943, 1296, 1621
DraIII CACNNNGTG 1 cut(s) 1729
DriI GACNNNNNGTC 1 cut(s) 2102
EaeI YGGCCR 1 cut(s) 421
Eam1104I CTCTTC 3 cut(s) 46, 257, 1296
Eam1105I GACNNNNNGTC 1 cut(s) 2102
EarI CTCTTC 3 cut(s) 46, 257, 1296
EciI GGCGGA 1 cut(s) 163
Ecl136II GAGCTC 3 cut(s) 193, 239, 1511
Eco24I GRGCYC 3 cut(s) 195, 241, 1513
Eco31I GGTCTC 1 cut(s) 2050
Eco47I GGWCC 5 cut(s) 345, 1169, 1545, 1757, 2063
Eco53kI GAGCTC 3 cut(s) 193, 239, 1511
Eco57I CTGAAG 3 cut(s) 419, 1017, 1185
Eco88I CYCGRG 1 cut(s) 1593
EcoICRI GAGCTC 3 cut(s) 193, 239, 1511
EcoO109I RGGNCCY 3 cut(s) 345, 1169, 1757
EcoRII CCWGG 7 cut(s) 532, 818, 948, 1378, 1828, 1926, 1959
EcoT22I ATGCAT 1 cut(s) 1669
EcoT38I GRGCYC 3 cut(s) 195, 241, 1513
FaeI CATG 6 cut(s) 23, 475, 620, 1715, 1754, 1850
FalI AAGNNNNNCTT 2 cut(s) 393, 425
FaqI GGGAC 2 cut(s) 1177, 2051
FatI CATG 6 cut(s) 19, 471, 616, 1711, 1750, 1846
FauI CCCGC 2 cut(s) 468, 952
FauNDI CATATG 2 cut(s) 979, 1948
FbaI TGATCA 1 cut(s) 1621
FokI GGATG 6 cut(s) 314, 665, 1052, 1255, 1277, 1943
FriOI GRGCYC 3 cut(s) 195, 241, 1513
FspBI CTAG 1 cut(s) 1388
GlaI GCGC 2 cut(s) 206, 1281
GsuI CTGGAG 1 cut(s) 971
HaeII RGCGCY 1 cut(s) 208
HaeIII GGCC 4 cut(s) 423, 530, 976, 1628
HapII CCGG 4 cut(s) 163, 264, 375, 1649
HgaI GACGC 5 cut(s) 17, 34, 256, 287, 1032
HhaI GCGC 2 cut(s) 207, 1282
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 6 cut(s) 23, 475, 620, 1715, 1754, 1850
Hin6I GCGC 2 cut(s) 205, 1280
HinP1I GCGC 2 cut(s) 205, 1280
HincII GTYRAC 1 cut(s) 1894
HindII GTYRAC 1 cut(s) 1894
HindIII AAGCTT 1 cut(s) 1396
HinfI GANTC 9 cut(s) 218, 224, 273, 466, 613, 668, 983, 1148, 1870
HpaII CCGG 4 cut(s) 163, 264, 375, 1649
HphI GGTGA 6 cut(s) 299, 849, 938, 1571, 1582, 2040
Hpy166II GTNNAC 5 cut(s) 706, 1069, 1522, 1562, 1894
Hpy188III TCNNGA 8 cut(s) 157, 163, 299, 617, 672, 1358, 1619, 1751
Hpy8I GTNNAC 5 cut(s) 706, 1069, 1522, 1562, 1894
Hpy99I CGWCG 4 cut(s) 28, 190, 272, 281
HpyAV CCTTC 5 cut(s) 55, 1160, 1232, 1575, 1861
HpyCH4III ACNGT 2 cut(s) 1549, 1727
HpyCH4IV ACGT 1 cut(s) 1573
HpyF10VI GCNNNNNNNGC 5 cut(s) 380, 734, 1022, 1277, 1430
HpyF3I CTNAG 9 cut(s) 297, 393, 452, 933, 1134, 1230, 1332, 1512, 1660
HpySE526I ACGT 1 cut(s) 1573
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 6 cut(s) 23, 475, 620, 1715, 1754, 1850
HspAI GCGC 2 cut(s) 205, 1280
Kpn2I TCCGGA 1 cut(s) 162
KroI GCCGGC 1 cut(s) 374
KroNI GCCGGC 1 cut(s) 376
Ksp22I TGATCA 1 cut(s) 1621
Kzo9I GATC 5 cut(s) 159, 888, 943, 1296, 1621
LguI GCTCTTC 1 cut(s) 1296
LmnI GCTCC 6 cut(s) 165, 190, 198, 237, 1508, 1733
MaeI CTAG 1 cut(s) 1388
MaeII ACGT 1 cut(s) 1573
MaeIII GTNAC 1 cut(s) 388
MalI GATC 5 cut(s) 161, 890, 945, 1298, 1623
MboI GATC 5 cut(s) 159, 888, 943, 1296, 1621
MboII GAAGA 8 cut(s) 33, 244, 425, 561, 596, 1313, 1702, 1853
MhlI GDGCHC 4 cut(s) 195, 241, 1513, 1725
MluI ACGCGT 1 cut(s) 26
MlyI GAGTC 4 cut(s) 212, 218, 977, 1142
MmeI TCCRAC 9 cut(s) 168, 220, 240, 246, 250, 443, 921, 1017, 1479
Mph1103I ATGCAT 1 cut(s) 1669
MroI TCCGGA 1 cut(s) 162
MroNI GCCGGC 1 cut(s) 374
MroXI GAANNNNTTC 1 cut(s) 1353
MseI TTAA 3 cut(s) 1259, 1742, 2013
MslI CAYNNNNRTG 5 cut(s) 312, 935, 1178, 1557, 2074
MspA1I CMGCKG 2 cut(s) 451, 776
MspI CCGG 4 cut(s) 163, 264, 375, 1649
MspR9I CCNGG 9 cut(s) 264, 534, 820, 950, 1380, 1650, 1830, 1928, 1961
Mva1269I GAATGC 4 cut(s) 961, 1060, 1667, 1857
MvaI CCWGG 7 cut(s) 534, 820, 950, 1380, 1830, 1928, 1961
MvnI CGCG 2 cut(s) 28, 246
MwoI GCNNNNNNNGC 5 cut(s) 380, 734, 1022, 1277, 1430
NaeI GCCGGC 1 cut(s) 376
NciI CCSGG 2 cut(s) 264, 1650
NdeI CATATG 2 cut(s) 979, 1948
NdeII GATC 5 cut(s) 159, 888, 943, 1296, 1621
NgoMIV GCCGGC 1 cut(s) 374
NlaIII CATG 6 cut(s) 23, 475, 620, 1715, 1754, 1850
NlaIV GGNNCC 3 cut(s) 531, 1171, 1758
NmuCI GTSAC 1 cut(s) 388
NsiI ATGCAT 1 cut(s) 1669
OliI CACNNNNGTG 2 cut(s) 312, 935
PagI TCATGA 2 cut(s) 616, 1750
PciSI GCTCTTC 1 cut(s) 1296
PctI GAATGC 4 cut(s) 961, 1060, 1667, 1857
PdiI GCCGGC 1 cut(s) 376
PdmI GAANNNNTTC 1 cut(s) 1353
PfeI GAWTC 5 cut(s) 273, 466, 613, 668, 1870
PflFI GACNNNGTC 1 cut(s) 2042
PleI GAGTC 4 cut(s) 212, 218, 977, 1142
PpsI GAGTC 4 cut(s) 212, 218, 977, 1142
PpuMI RGGWCCY 3 cut(s) 345, 1169, 1757
PsiI TTATAA 1 cut(s) 1446
Psp124BI GAGCTC 3 cut(s) 195, 241, 1513
Psp5II RGGWCCY 3 cut(s) 345, 1169, 1757
Psp6I CCWGG 7 cut(s) 532, 818, 948, 1378, 1828, 1926, 1959
PspGI CCWGG 7 cut(s) 532, 818, 948, 1378, 1828, 1926, 1959
PspN4I GGNNCC 3 cut(s) 531, 1171, 1758
PspPI GGNCC 6 cut(s) 345, 529, 1169, 1545, 1757, 2063
PspPPI RGGWCCY 3 cut(s) 345, 1169, 1757
PstI CTGCAG 2 cut(s) 583, 1348
PsyI GACNNNGTC 1 cut(s) 2042
PvuII CAGCTG 2 cut(s) 451, 776
RsaI GTAC 3 cut(s) 495, 705, 1551
RsaNI GTAC 3 cut(s) 494, 704, 1550
RseI CAYNNNNRTG 5 cut(s) 312, 935, 1178, 1557, 2074
SacI GAGCTC 3 cut(s) 195, 241, 1513
SapI GCTCTTC 1 cut(s) 1296
SaqAI TTAA 3 cut(s) 1259, 1742, 2013
Sau3AI GATC 5 cut(s) 159, 888, 943, 1296, 1621
Sau96I GGNCC 6 cut(s) 345, 529, 1169, 1545, 1757, 2063
SchI GAGTC 4 cut(s) 212, 218, 977, 1142
ScrFI CCNGG 9 cut(s) 264, 534, 820, 950, 1380, 1650, 1830, 1928, 1961
SduI GDGCHC 4 cut(s) 195, 241, 1513, 1725
SfcI CTRYAG 2 cut(s) 579, 1344
SinI GGWCC 5 cut(s) 345, 1169, 1545, 1757, 2063
SmiMI CAYNNNNRTG 5 cut(s) 312, 935, 1178, 1557, 2074
SsiI CCGC 8 cut(s) 70, 174, 244, 424, 475, 959, 1246, 1997
SspI AATATT 1 cut(s) 1097
SspMI CTAG 1 cut(s) 1388
SstI GAGCTC 3 cut(s) 195, 241, 1513
StyD4I CCNGG 9 cut(s) 262, 532, 818, 948, 1378, 1648, 1828, 1926, 1959
TaaI ACNGT 2 cut(s) 1549, 1727
TaiI ACGT 1 cut(s) 1576
TaqI TCGA 6 cut(s) 276, 458, 891, 1299, 1777, 2058
TatI WGTACW 1 cut(s) 703
TauI GCSGC 1 cut(s) 426
TfiI GAWTC 5 cut(s) 273, 466, 613, 668, 1870
Tru1I TTAA 3 cut(s) 1259, 1742, 2013
Tru9I TTAA 3 cut(s) 1259, 1742, 2013
TscAI CASTG 6 cut(s) 385, 937, 1180, 1221, 1417, 1474
TseFI GTSAC 1 cut(s) 388
Tsp45I GTSAC 1 cut(s) 388
TspDTI ATGAA 9 cut(s) 64, 310, 669, 771, 825, 1494, 1739, 1839, 1854
TspGWI ACGGA 1 cut(s) 1539
TspRI CASTG 6 cut(s) 385, 937, 1180, 1221, 1417, 1474
Tth111I GACNNNGTC 1 cut(s) 2042
VpaK11BI GGWCC 5 cut(s) 345, 1169, 1545, 1757, 2063
XapI RAATTY 3 cut(s) 829, 1361, 2000
XmnI GAANNNNTTC 1 cut(s) 1353
XspI CTAG 1 cut(s) 1388
Zsp2I ATGCAT 1 cut(s) 1669
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.