RchiOBHm_Chr2g0093251

isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0006067
Physical Location & Seq
Reverse (-)
6534758 .. 6538114
3357 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1593 bp
ATGCCGGGAACCATCTTGGTTTCAGTTCTTGAATTCATGGACCTTCCATTTTCATCATCATCTTCTTCAATGTCGATAAAGGTATCTTTGGGGTCAAGATCAGAGTACCAAATTTGGGAGAGTAGAGACTTGTCCTTCCCATTGACGACTCTTCGGGACAACATGCTTGTTATTCTCCAAGATTCTGAAGGGAATCAGATATCAGAAACAGGTATTGAGACTAGGTCCATAGTTGAAAAGGGTCTTTGGGATGACTTCTTCCCCCTCAAAGGCGGTGGGTTTCTGCATATGAAATTGCAGTTTTTACTCAATGAAGAAGAGCGCAATCAAATCCGTGTGATGCGAGAATCTGCTGTGAAAAAGAAGCATGGGGAGTTCATTCATAGCAGTCCTATTAGTGTGCAAAGTACTCCTGGTGCCCGGATCAATGTTGCATCATCTTTGAGTCTTCACAATAGTGATGCAGATAGGAATGAAGAGACTTCATCAGACATCACTGTGCAGCAAAGTGTTAATTTGTCTCCAACTGAATTGAGTCGCAGGGAGTTGGTTGATAACATCGCTGCCCAACCATTTCATACTAAGATTCCTGCCAAAGCCATTTGTGCTGATGAACCTGCAAGGTTATTAAGAGGCTCACAACCAGTTGTTGATAACCTTCCTAAGCTTCAGGAAGAAAAGCATTGCAAACTCGAAAAGCAGCAGAGCCTGCTGAAGAAAACTCCTAGCGTAAAGAACATGATAAGTGCCTTTGAAACTGGTTTAGCGGAGGATGAGAAGCCTCCCATCAAACCAGCACCAATGAAAGTTCAATCAAACAAAACTATAACAGGCAATCTTCCAAAAAACCAGGACTTAAAAGAAGACAAGAAAGTCGGTACAGAAACTTCAGAGTCGATATCAAAACGAGCAGTGAATCCTTTACCTCCAGAACAGTTGCAACATGACACGACATGTGGTGAAAGCGGTGAAGAAAATATTTCACTTGGAGCTTTAGGTGGGACTAAATCATCACACGACACAGTTAAAATTTCAGAGAGGGTACTTCATGATCATAGACACCAACCTAGTAAGATGCTCCATGGAAAAAGAGATTCTGGTGGCAGTCCAGTTATAGAGGAGACTCATCAGCTAGAAATCTCTTCCAGAAATTCTCAAATGTCCGACATTCAAGGACTTGCAGCCCAAATATGTACTTCAATAGCAAACTGCGAAGATAGGCACAATCCCTTTCAAAGCTCTAGTGGATGGATATTCCCAGATGATGCAATACAATTTTGTATAACAACTAGTGGTAAAAAGGTGATGGATTTTCTGGGAAGTTGCAGTATGATGAAGCCAAGTATCCATCAAGATAAAAAGAGTTTGTCTTCCTCAAAAAATGCAGAAGACCAGCAGGATAACGTGGATAGCGGTAATAGTAAAGAAGTGAACAGAAATGAGAAGAATCACAGACCAAAAGAACTACATAAGGTTCAGAAATCAGAAGAAACAGAGACTTCTGGGGTGGCGGTTGGACAGGCAATAAAAGTTGCAATTATGATTGGATTTGGAACACTTGTTCTTCTCACTAGACAAAGGAAAAATATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

530

Amino Acids

58.59

Weight (kDa)

6.4

Isoelectric Point (pI)

59.05

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 872
Acc36I ACCTGC 1 cut(s) 625
AccB1I GGYRCC 1 cut(s) 416
AciI CCGC 5 cut(s) 273, 767, 966, 1413, 1511
AclWI GGATC 1 cut(s) 431
AcsI RAATTY 4 cut(s) 32, 111, 1029, 1150
AcuI CTGAAG 4 cut(s) 207, 653, 734, 873
AfaI GTAC 5 cut(s) 107, 409, 880, 1044, 1195
AfiI CCNNNNNNNGG 2 cut(s) 115, 269
AflIII ACRYGT 1 cut(s) 953
AgsI TTSAA 8 cut(s) 32, 69, 236, 755, 812, 1172, 1200, 1235
AhlI ACTAGT 1 cut(s) 1289
AjnI CCWGG 2 cut(s) 412, 849
AleI CACNNNNGTG 1 cut(s) 456
AloI GAACNNNNNNTCC 2 cut(s) 1545, 1577
AluBI AGCT 4 cut(s) 667, 992, 1132, 1239
AluI AGCT 4 cut(s) 667, 992, 1132, 1239
Alw26I GTCTC 6 cut(s) 120, 212, 473, 525, 1115, 1490
AlwI GGATC 1 cut(s) 431
AlwNI CAGNNNCTG 1 cut(s) 709
ApeKI GCWGC 4 cut(s) 502, 563, 700, 1181
ApoI RAATTY 4 cut(s) 32, 111, 1029, 1150
AspLEI GCGC 1 cut(s) 324
AspS9I GGNCC 2 cut(s) 40, 225
AsuC2I CCSGG 2 cut(s) 6, 421
AsuHPI GGTGA 3 cut(s) 971, 980, 1315
AvaII GGWCC 2 cut(s) 40, 225
BaeGI GKGCMC 1 cut(s) 421
BanI GGYRCC 1 cut(s) 416
BarI GAAGNNNNNNTAC 2 cut(s) 871, 903
BbsI GAAGAC 4 cut(s) 440, 870, 1362, 1395
BbvI GCAGC 4 cut(s) 514, 550, 712, 1193
BccI CCATC 5 cut(s) 20, 794, 1242, 1300, 1356
BciT130I CCWGG 2 cut(s) 414, 851
BciVI GTATCC 1 cut(s) 1355
BclI TGATCA 1 cut(s) 1051
BcnI CCSGG 2 cut(s) 6, 421
BcoDI GTCTC 6 cut(s) 120, 212, 473, 525, 1115, 1490
BcuI ACTAGT 1 cut(s) 1289
BfaI CTAG 7 cut(s) 222, 726, 1068, 1133, 1242, 1290, 1572
BfuAI ACCTGC 1 cut(s) 625
BfuI GTATCC 1 cut(s) 1355
BisI GCNGC 4 cut(s) 503, 564, 701, 1182
BlsI GCNGC 4 cut(s) 504, 565, 702, 1183
BmcAI AGTACT 1 cut(s) 409
Bme1390I CCNGG 4 cut(s) 6, 414, 421, 851
Bme18I GGWCC 2 cut(s) 40, 225
BmgT120I GGNCC 2 cut(s) 40, 225
BmiI GGNNCC 2 cut(s) 10, 418
BmrFI CCNGG 4 cut(s) 6, 414, 421, 851
BmsI GCATC 5 cut(s) 330, 443, 451, 1065, 1255
BpiI GAAGAC 4 cut(s) 440, 870, 1362, 1395
BpmI CTGGAG 1 cut(s) 912
Bpu10I CCTNAGC 1 cut(s) 663
BpuMI CCSGG 2 cut(s) 6, 421
BsaJI CCNNGG 1 cut(s) 1081
Bsc4I CCNNNNNNNGG 2 cut(s) 115, 269
Bse1I ACTGG 3 cut(s) 644, 763, 1109
Bse3DI GCAATG 1 cut(s) 682
BseBI CCWGG 2 cut(s) 414, 851
BseDI CCNNGG 1 cut(s) 1081
BseGI GGATG 3 cut(s) 256, 778, 1253
BseLI CCNNNNNNNGG 2 cut(s) 115, 269
BseMI GCAATG 1 cut(s) 682
BseNI ACTGG 3 cut(s) 644, 763, 1109
BseRI GAGGAG 1 cut(s) 1133
BseSI GKGCMC 1 cut(s) 421
BseXI GCAGC 4 cut(s) 514, 550, 712, 1193
BsgI GTGCAG 1 cut(s) 521
BshNI GGYRCC 1 cut(s) 416
BsiSI CCGG 2 cut(s) 5, 421
BslFI GGGAC 2 cut(s) 170, 1015
BslI CCNNNNNNNGG 2 cut(s) 115, 269
BsmAI GTCTC 6 cut(s) 120, 212, 473, 525, 1115, 1490
BsmFI GGGAC 2 cut(s) 170, 1015
Bsp1286I GDGCHC 1 cut(s) 421
Bsp143I GATC 3 cut(s) 98, 423, 1051
Bsp19I CCATGG 1 cut(s) 1081
BspACI CCGC 5 cut(s) 273, 767, 966, 1413, 1511
BspHI TCATGA 1 cut(s) 1048
BspLI GGNNCC 2 cut(s) 10, 418
BspMI ACCTGC 1 cut(s) 625
BspPI GGATC 1 cut(s) 431
BspQI GCTCTTC 1 cut(s) 312
BspT107I GGYRCC 1 cut(s) 416
BsrDI GCAATG 1 cut(s) 682
BsrI ACTGG 3 cut(s) 644, 763, 1109
BssECI CCNNGG 1 cut(s) 1081
BssMI GATC 3 cut(s) 98, 423, 1051
BssT1I CCWWGG 1 cut(s) 1081
Bst2UI CCWGG 2 cut(s) 414, 851
Bst4CI ACNGT 3 cut(s) 499, 936, 1024
Bst6I CTCTTC 4 cut(s) 156, 312, 471, 1147
BstAPI GCANNNNNTGC 1 cut(s) 709
BstC8I GCNNGC 1 cut(s) 710
BstDEI CTNAG 2 cut(s) 582, 663
BstDSI CCRYGG 1 cut(s) 1081
BstF5I GGATG 3 cut(s) 256, 778, 1253
BstHHI GCGC 1 cut(s) 324
BstKTI GATC 3 cut(s) 101, 426, 1054
BstMAI GTCTC 6 cut(s) 120, 212, 473, 525, 1115, 1490
BstMBI GATC 3 cut(s) 98, 423, 1051
BstMWI GCNNNNNNNGC 2 cut(s) 605, 709
BstNI CCWGG 2 cut(s) 414, 851
BstNSI RCATGY 2 cut(s) 166, 957
BstSCI CCNGG 4 cut(s) 4, 412, 419, 849
BstSLI GKGCMC 1 cut(s) 421
BstV1I GCAGC 4 cut(s) 514, 550, 712, 1193
BstV2I GAAGAC 4 cut(s) 440, 870, 1362, 1395
BsuI GTATCC 1 cut(s) 1355
BtgI CCRYGG 1 cut(s) 1081
BtgZI GCGATG 1 cut(s) 544
BtsCI GGATG 3 cut(s) 256, 778, 1253
BtsI GCAGTG 1 cut(s) 918
BtsIMutI CAGTG 2 cut(s) 495, 918
BveI ACCTGC 1 cut(s) 625
Cac8I GCNNGC 1 cut(s) 710
CaiI CAGNNNCTG 1 cut(s) 709
CciI TCATGA 1 cut(s) 1048
CfoI GCGC 1 cut(s) 324
Cfr13I GGNCC 2 cut(s) 40, 225
Csp6I GTAC 5 cut(s) 106, 408, 879, 1043, 1194
CspCI CAANNNNNGTGG 2 cut(s) 256, 291
CviAII CATG 8 cut(s) 37, 163, 368, 739, 944, 954, 1049, 1082
CviQI GTAC 5 cut(s) 106, 408, 879, 1043, 1194
DdeI CTNAG 2 cut(s) 582, 663
DpnI GATC 3 cut(s) 100, 425, 1053
DpnII GATC 3 cut(s) 98, 423, 1051
DrdI GACNNNNNNGTC 1 cut(s) 872
DseDI GACNNNNNNGTC 1 cut(s) 872
Eam1104I CTCTTC 4 cut(s) 156, 312, 471, 1147
EarI CTCTTC 4 cut(s) 156, 312, 471, 1147
Eco130I CCWWGG 1 cut(s) 1081
Eco32I GATATC 2 cut(s) 201, 900
Eco47I GGWCC 2 cut(s) 40, 225
Eco57I CTGAAG 4 cut(s) 207, 653, 734, 873
EcoRI GAATTC 1 cut(s) 32
EcoRII CCWGG 2 cut(s) 412, 849
EcoRV GATATC 2 cut(s) 201, 900
EcoT14I CCWWGG 1 cut(s) 1081
ErhI CCWWGG 1 cut(s) 1081
FaeI CATG 8 cut(s) 40, 166, 371, 742, 947, 957, 1052, 1085
FaqI GGGAC 2 cut(s) 170, 1015
FatI CATG 8 cut(s) 36, 162, 367, 738, 943, 953, 1048, 1081
FauNDI CATATG 1 cut(s) 288
FbaI TGATCA 1 cut(s) 1051
Fnu4HI GCNGC 4 cut(s) 503, 564, 701, 1182
FokI GGATG 3 cut(s) 263, 785, 1260
Fsp4HI GCNGC 4 cut(s) 503, 564, 701, 1182
FspBI CTAG 7 cut(s) 222, 726, 1068, 1133, 1242, 1290, 1572
GlaI GCGC 1 cut(s) 323
GluI GCNGC 4 cut(s) 503, 564, 701, 1182
GsuI CTGGAG 1 cut(s) 912
HapII CCGG 2 cut(s) 5, 421
HhaI GCGC 1 cut(s) 324
Hin1II CATG 8 cut(s) 40, 166, 371, 742, 947, 957, 1052, 1085
Hin6I GCGC 1 cut(s) 322
HinP1I GCGC 1 cut(s) 322
HindIII AAGCTT 1 cut(s) 665
HpaII CCGG 2 cut(s) 5, 421
HphI GGTGA 3 cut(s) 971, 980, 1315
Hpy166II GTNNAC 1 cut(s) 1432
Hpy188III TCNNGA 8 cut(s) 29, 96, 155, 671, 929, 1049, 1146, 1352
Hpy8I GTNNAC 1 cut(s) 1432
HpyAV CCTTC 4 cut(s) 53, 145, 182, 668
HpyCH4III ACNGT 3 cut(s) 499, 936, 1024
HpyCH4IV ACGT 1 cut(s) 1404
HpyF10VI GCNNNNNNNGC 2 cut(s) 605, 709
HpyF3I CTNAG 2 cut(s) 582, 663
HpySE526I ACGT 1 cut(s) 1404
Hsp92II CATG 8 cut(s) 40, 166, 371, 742, 947, 957, 1052, 1085
HspAI GCGC 1 cut(s) 322
Ksp22I TGATCA 1 cut(s) 1051
Kzo9I GATC 3 cut(s) 98, 423, 1051
LguI GCTCTTC 1 cut(s) 312
LmnI GCTCC 2 cut(s) 989, 1083
Lsp1109I GCAGC 4 cut(s) 514, 550, 712, 1193
LweI GCATC 5 cut(s) 330, 443, 451, 1065, 1255
MaeI CTAG 7 cut(s) 222, 726, 1068, 1133, 1242, 1290, 1572
MaeII ACGT 1 cut(s) 1404
MalI GATC 3 cut(s) 100, 425, 1053
MboI GATC 3 cut(s) 98, 423, 1051
MhlI GDGCHC 1 cut(s) 421
MluCI AATT 9 cut(s) 32, 111, 293, 514, 530, 1029, 1150, 1274, 1536
MlyI GAGTC 5 cut(s) 142, 454, 544, 902, 1117
MmeI TCCRAC 3 cut(s) 548, 1188, 1495
MnlI CCTC 8 cut(s) 275, 626, 763, 792, 936, 1032, 1111, 1384
MseI TTAA 4 cut(s) 513, 629, 857, 1026
MslI CAYNNNNRTG 2 cut(s) 456, 497
MspI CCGG 2 cut(s) 5, 421
MspR9I CCNGG 4 cut(s) 6, 414, 421, 851
MvaI CCWGG 2 cut(s) 414, 851
MwoI GCNNNNNNNGC 2 cut(s) 605, 709
NciI CCSGG 2 cut(s) 6, 421
NcoI CCATGG 1 cut(s) 1081
NdeI CATATG 1 cut(s) 288
NdeII GATC 3 cut(s) 98, 423, 1051
NlaIII CATG 8 cut(s) 40, 166, 371, 742, 947, 957, 1052, 1085
NlaIV GGNNCC 2 cut(s) 10, 418
NspI RCATGY 2 cut(s) 166, 957
OliI CACNNNNGTG 1 cut(s) 456
PagI TCATGA 1 cut(s) 1048
PciI ACATGT 1 cut(s) 953
PciSI GCTCTTC 1 cut(s) 312
PfeI GAWTC 7 cut(s) 182, 193, 347, 586, 916, 1094, 1447
PflFI GACNNNGTC 1 cut(s) 223
PkrI GCNGC 4 cut(s) 504, 565, 702, 1183
PleI GAGTC 5 cut(s) 142, 453, 543, 901, 1117
PpsI GAGTC 5 cut(s) 142, 453, 543, 901, 1117
PscI ACATGT 1 cut(s) 953
Psp6I CCWGG 2 cut(s) 412, 849
PspGI CCWGG 2 cut(s) 412, 849
PspN4I GGNNCC 2 cut(s) 10, 418
PspPI GGNCC 2 cut(s) 40, 225
PstNI CAGNNNCTG 1 cut(s) 709
PsyI GACNNNGTC 1 cut(s) 223
RsaI GTAC 5 cut(s) 107, 409, 880, 1044, 1195
RsaNI GTAC 5 cut(s) 106, 408, 879, 1043, 1194
RseI CAYNNNNRTG 2 cut(s) 456, 497
SapI GCTCTTC 1 cut(s) 312
SaqAI TTAA 4 cut(s) 513, 629, 857, 1026
SatI GCNGC 4 cut(s) 503, 564, 701, 1182
Sau3AI GATC 3 cut(s) 98, 423, 1051
Sau96I GGNCC 2 cut(s) 40, 225
ScaI AGTACT 1 cut(s) 409
SchI GAGTC 5 cut(s) 142, 454, 544, 902, 1117
ScrFI CCNGG 4 cut(s) 6, 414, 421, 851
SduI GDGCHC 1 cut(s) 421
SfaNI GCATC 5 cut(s) 330, 443, 451, 1065, 1255
SinI GGWCC 2 cut(s) 40, 225
SmiMI CAYNNNNRTG 2 cut(s) 456, 497
SpeI ACTAGT 1 cut(s) 1289
Sse9I AATT 9 cut(s) 32, 111, 293, 514, 530, 1029, 1150, 1274, 1536
SsiI CCGC 5 cut(s) 273, 767, 966, 1413, 1511
SspI AATATT 1 cut(s) 979
SspMI CTAG 7 cut(s) 222, 726, 1068, 1133, 1242, 1290, 1572
StyD4I CCNGG 4 cut(s) 4, 412, 419, 849
StyI CCWWGG 1 cut(s) 1081
TaaI ACNGT 3 cut(s) 499, 936, 1024
TaiI ACGT 1 cut(s) 1407
TaqI TCGA 3 cut(s) 74, 693, 896
TasI AATT 9 cut(s) 32, 111, 293, 514, 530, 1029, 1150, 1274, 1536
TatI WGTACW 2 cut(s) 407, 1193
TfiI GAWTC 7 cut(s) 182, 193, 347, 586, 916, 1094, 1447
Tru1I TTAA 4 cut(s) 513, 629, 857, 1026
Tru9I TTAA 4 cut(s) 513, 629, 857, 1026
TscAI CASTG 2 cut(s) 502, 918
TseI GCWGC 4 cut(s) 502, 563, 700, 1181
TspGWI ACGGA 1 cut(s) 323
TspRI CASTG 2 cut(s) 502, 918
Tth111I GACNNNGTC 1 cut(s) 223
VpaK11BI GGWCC 2 cut(s) 40, 225
XapI RAATTY 4 cut(s) 32, 111, 1029, 1150
XceI RCATGY 2 cut(s) 166, 957
XspI CTAG 7 cut(s) 222, 726, 1068, 1133, 1242, 1290, 1572
ZrmI AGTACT 1 cut(s) 409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.