RchiOBHm_Chr2g0102141

Cupin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0012303
Physical Location & Seq
Reverse (-)
13582162 .. 13585328
3167 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1845 bp
ATGGTTCAATATAAAATGTCTAACAACGATTCAAATCCACCGTTTTCACTTACTCTGTTCATCTTATTCTTCTTTCTTTCACTACCTGCCTTCTCTTCCTCTTTGAAGTCTTTGAAAAATGAGGAGGATTTTTTCAGTAGTGATGCCGGGTATCTGGTGAGGAGAGCTCAGAGGAGGGCGTTGGTTCAGACAGAGTACGGAGAAATCTCGTCCATTGATGTCACCGATGGACGGAGAGGGCCTTACCATGTCCAGTTCATCATCATGGAACCCAACTCGCTGTTCCTTCCGGTGCTGCTACACTCTGACATGGTGTTCTATGTCCACACAGGGAGTGGGAGGTTGAATTGGGCTCATGGAAAGGATGATCAAATCAACGAGGTTCCTTTAAAGCAAGGTGATATCCATAGACTCCGCCCAGGATCTGTCTTCTTTGTTCAGAGTGATTTACAGACCGAGCGTCAGAAACTTAGAATTAATGCAATCTTTGCAAATAGCGATGATGACGTATTTGACCCAACAATAGGAGCATACTCCAGTGTTAGGGACCTGGTCCGAGGCTTCGACACCAAAATCCTTCGGTCAGCTTTTCAGGTTTCTGAGGATGTGATCAAGTCGATAACAAATTCAACAGACCAATCAGCCATTATCCATGCAGTGCCAACGAAGAAAGAAACCTTTTGGGATTTGGAAGCTCGGTTCTTGAAAACCTTTATAGGAGCCAGTTATGATGGCATGGCATTAAACAAGAAGAAGCATAAGAAGAAAGAAAAATCAAAAACATATAATATTTTTGATGAGGACCCAGATTTCAAGAATTGTAACGGTTGGAGCCTCACCGTGAATGAGAGAAACTCGCCTTCATTGACTGGATCAAACATTGGTCTTTTCATGGTGAACTTGACGAAGGGTTCAATGATGGGGCCTCATTGGAATCCAAGGGCAACTGAGATCGCAGTTGTGTTACAGGGGCAAGGAATGGTCAGGGTGGTATGTGCTAGCACAGCAAAGAGATCAGAATGCAGAAATTTGAGGTTTAGGGTTCATGAAGGAGATGTATTTGCTGTACCTATGTTCCATCCAATGGCTCAGATGTCATATAATAATGACTCATTTGTGTTTATGGGGTTCAGCACGACAACAAGGAGAAATTATCCTCAATTTTTGGCTGGGAAATACTCGGTTCTCCAAACTTTGAACAGACAGGTCTTGGCAGTGTCTTTGAATGTTACCAACACCACAATCGATCAGCTTTTGGCTGCACAAACAGAATCAATCATACTCGACTGTACTTCTTGTGCTGAAAAAGAGGAGAAAGTAATGTTGGAAGAAATTGAAAAGGAAAGGGAGGAAGAGGAGGCTAAGAAGAGAGAGGAACAGAGGAAGAGAGAAGAGGAGCAACGGAAGAGAGAGGAGGAGCAGAGACAGAGAGAGGAGGAGGAAGCGAGAAAGAAAGAAGAGGAAGAAGAGAGGAGAAAGAGAGAAGAGGAAGAAGAGAGGAGGAAGAGAGAAGAGGAGGAAGAAGAAGAGAGGAGAAGGAGAGAAGAAGAAAGAAGAGAGGAGGAAGAACAAGAGAGGGAGAGGCAACAGGAAGAAGGAGGAGAAGCAGCAGAAAGGGAGCAAGAAGAGGCTAGGAGGCAAGAGGAAGAAGCAGAGAGTGAGAAAGAAGACACCAGGAGGCAACAACAAGAGGAGTACAGTAAAAGAAAAGAGAAAGAGGCCCAAACAGAGCAAGAGGAGGCTAGGAAACAACAAGAGGAAATAGAAGGAAGAACCAGACCGGAGGAGCAAGAGACGGAAGGTGACCAACGTTTTGACGGTGAAGCGGGAAGAAGAGAAATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0006950 GO:0006952 GO:0008150 GO:0009605 GO:0009607 GO:0009620 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

614

Amino Acids

71.9

Weight (kDa)

5.17

Isoelectric Point (pI)

81.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 262 - 415 5.8e-38 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012303)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G36700
fragaria_vesca FvH4_1g13730 FvH4_1g13730 FvH4_1g13730
malus_domestica MD02G1152000.v1.1 MD15G1266400.v1.1
prunus_persica Prupe.7G151000_v2.0.a1 Prupe.7G151000_v2.0.a1
pyrus_communis pycom02g11940
rosa_chinensis RchiOBHm_Chr2g0102141
rosa_laevigata RLG00000017133
rosa_roxburghii Rroxscaffold_2G00140750
rosa_rugosa Rorug02G0104600 Rorug02G0104700
rosa_samantha Rh2AG153100 Rh2BG159000 Rh2CG158300 Rh2DG158700
rosa_wichuraiana Rw2G011950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 94
AccB7I CCANNNNNTGG 1 cut(s) 1084
AciI CCGC 2 cut(s) 415, 1826
AclI AACGTT 1 cut(s) 1810
AclWI GGATC 2 cut(s) 430, 880
AcsI RAATTY 3 cut(s) 625, 1027, 1839
AfaI GTAC 4 cut(s) 197, 1068, 1291, 1697
AfiI CCNNNNNNNGG 4 cut(s) 231, 524, 543, 1084
AhdI GACNNNNNGTC 1 cut(s) 459
AjnI CCWGG 3 cut(s) 418, 549, 1673
AjuI GAANNNNNNNTTGG 4 cut(s) 266, 298, 1307, 1339
AloI GAACNNNNNNTCC 2 cut(s) 683, 715
AluBI AGCT 4 cut(s) 167, 587, 695, 1252
AluI AGCT 4 cut(s) 167, 587, 695, 1252
Alw21I GWGCWC 1 cut(s) 169
Alw26I GTCTC 2 cut(s) 1417, 1787
AlwI GGATC 2 cut(s) 430, 880
AlwNI CAGNNNCTG 1 cut(s) 425
AoxI GGCC 3 cut(s) 239, 923, 1719
ApeKI GCWGC 3 cut(s) 295, 1259, 1607
ApoI RAATTY 3 cut(s) 625, 1027, 1839
ArsI GACNNNNNNTTYG 2 cut(s) 1796, 1828
AseI ATTAAT 1 cut(s) 477
AspS9I GGNCC 6 cut(s) 239, 547, 553, 802, 923, 1720
AsuC2I CCSGG 1 cut(s) 148
AsuHPI GGTGA 7 cut(s) 169, 214, 410, 829, 907, 1814, 1832
AsuNHI GCTAGC 1 cut(s) 998
AvaII GGWCC 3 cut(s) 547, 553, 802
BanII GRGCYC 2 cut(s) 169, 355
BbsI GAAGAC 2 cut(s) 421, 1674
Bbv12I GWGCWC 1 cut(s) 169
BbvI GCAGC 3 cut(s) 282, 1246, 1619
BccI CCATC 4 cut(s) 221, 725, 913, 1086
BciT130I CCWGG 3 cut(s) 420, 551, 1675
BclI TGATCA 2 cut(s) 367, 609
BcnI CCSGG 1 cut(s) 148
BcoDI GTCTC 2 cut(s) 1417, 1787
BfaI CTAG 3 cut(s) 999, 1632, 1743
BfuAI ACCTGC 1 cut(s) 94
BisI GCNGC 3 cut(s) 296, 1260, 1608
BlsI GCNGC 3 cut(s) 297, 1261, 1609
Bme1390I CCNGG 4 cut(s) 148, 420, 551, 1675
Bme18I GGWCC 3 cut(s) 547, 553, 802
BmeRI GACNNNNNGTC 1 cut(s) 459
BmgT120I GGNCC 6 cut(s) 239, 547, 553, 802, 923, 1720
BmiI GGNNCC 7 cut(s) 270, 384, 548, 721, 804, 833, 924
BmrFI CCNGG 4 cut(s) 148, 420, 551, 1675
BmsI GCATC 1 cut(s) 133
BmtI GCTAGC 1 cut(s) 1002
BpiI GAAGAC 2 cut(s) 421, 1674
BplI GAGNNNNNCTC 4 cut(s) 151, 183, 839, 871
BpmI CTGGAG 1 cut(s) 520
BpuMI CCSGG 1 cut(s) 148
Bsa29I ATCGAT 1 cut(s) 1245
BsaBI GATNNNNATC 1 cut(s) 33
BsaJI CCNNGG 3 cut(s) 418, 556, 938
BsaWI WCCGGW 2 cut(s) 289, 1780
Bsc4I CCNNNNNNNGG 4 cut(s) 231, 524, 543, 1084
Bse1I ACTGG 4 cut(s) 253, 537, 723, 874
Bse8I GATNNNNATC 1 cut(s) 33
BseBI CCWGG 3 cut(s) 420, 551, 1675
BseCI ATCGAT 1 cut(s) 1245
BseDI CCNNGG 3 cut(s) 418, 556, 938
BseGI GGATG 3 cut(s) 370, 610, 1078
BseJI GATNNNNATC 1 cut(s) 33
BseLI CCNNNNNNNGG 4 cut(s) 231, 524, 543, 1084
BseMII CTCAG 4 cut(s) 182, 591, 939, 1103
BseNI ACTGG 4 cut(s) 253, 537, 723, 874
BseXI GCAGC 3 cut(s) 282, 1246, 1619
BseYI CCCAGC 1 cut(s) 1169
BsgI GTGCAG 1 cut(s) 1245
BshFI GGCC 3 cut(s) 241, 925, 1721
BshVI ATCGAT 1 cut(s) 1245
BsiHKAI GWGCWC 1 cut(s) 169
BsiSI CCGG 3 cut(s) 147, 290, 1781
BslFI GGGAC 1 cut(s) 560
BslI CCNNNNNNNGG 4 cut(s) 231, 524, 543, 1084
BsmAI GTCTC 2 cut(s) 1417, 1787
BsmBI CGTCTC 1 cut(s) 1787
BsmFI GGGAC 1 cut(s) 560
BsmI GAATGC 1 cut(s) 1025
BsnI GGCC 3 cut(s) 241, 925, 1721
Bsp1286I GDGCHC 2 cut(s) 169, 355
Bsp143I GATC 7 cut(s) 367, 422, 609, 872, 951, 1013, 1246
BspACI CCGC 2 cut(s) 415, 1826
BspANI GGCC 3 cut(s) 241, 925, 1721
BspCNI CTCAG 4 cut(s) 181, 592, 940, 1102
BspDI ATCGAT 1 cut(s) 1245
BspHI TCATGA 1 cut(s) 1045
BspLI GGNNCC 7 cut(s) 270, 384, 548, 721, 804, 833, 924
BspMI ACCTGC 1 cut(s) 94
BspOI GCTAGC 1 cut(s) 1002
BspPI GGATC 2 cut(s) 430, 880
BsrI ACTGG 4 cut(s) 253, 537, 723, 874
BssECI CCNNGG 3 cut(s) 418, 556, 938
BssMI GATC 7 cut(s) 367, 422, 609, 872, 951, 1013, 1246
BssT1I CCWWGG 1 cut(s) 938
Bst2UI CCWGG 3 cut(s) 420, 551, 1675
Bst4CI ACNGT 6 cut(s) 42, 827, 841, 1289, 1700, 1820
BstAPI GCANNNNNTGC 1 cut(s) 488
BstC8I GCNNGC 1 cut(s) 1000
BstDEI CTNAG 6 cut(s) 168, 470, 600, 948, 1089, 1362
BstEII GGTNACC 1 cut(s) 1802
BstF5I GGATG 3 cut(s) 370, 610, 1078
BstKTI GATC 7 cut(s) 370, 425, 612, 875, 954, 1016, 1249
BstMAI GTCTC 2 cut(s) 1417, 1787
BstMBI GATC 7 cut(s) 367, 422, 609, 872, 951, 1013, 1246
BstMWI GCNNNNNNNGC 2 cut(s) 488, 1004
BstNI CCWGG 3 cut(s) 420, 551, 1675
BstPI GGTNACC 1 cut(s) 1802
BstSCI CCNGG 4 cut(s) 146, 418, 549, 1673
BstV1I GCAGC 3 cut(s) 282, 1246, 1619
BstV2I GAAGAC 2 cut(s) 421, 1674
BstX2I RGATCY 1 cut(s) 422
BstYI RGATCY 1 cut(s) 422
Bsu15I ATCGAT 1 cut(s) 1245
BsuRI GGCC 3 cut(s) 241, 925, 1721
BsuTUI ATCGAT 1 cut(s) 1245
BtgZI GCGATG 1 cut(s) 513
BtsCI GGATG 3 cut(s) 370, 610, 1078
BtsI GCAGTG 2 cut(s) 663, 1221
BtsIMutI CAGTG 3 cut(s) 544, 663, 1221
BveI ACCTGC 1 cut(s) 94
Cac8I GCNNGC 1 cut(s) 1000
CaiI CAGNNNCTG 1 cut(s) 425
CciI TCATGA 1 cut(s) 1045
Cfr13I GGNCC 6 cut(s) 239, 547, 553, 802, 923, 1720
ClaI ATCGAT 1 cut(s) 1245
CseI GACGC 1 cut(s) 449
CsiI ACCWGGT 1 cut(s) 549
Csp6I GTAC 4 cut(s) 196, 1067, 1290, 1696
CviAII CATG 8 cut(s) 248, 265, 310, 356, 653, 736, 892, 1046
CviQI GTAC 4 cut(s) 196, 1067, 1290, 1696
DdeI CTNAG 6 cut(s) 168, 470, 600, 948, 1089, 1362
DpnI GATC 7 cut(s) 369, 424, 611, 874, 953, 1015, 1248
DpnII GATC 7 cut(s) 367, 422, 609, 872, 951, 1013, 1246
DraI TTTAAA 1 cut(s) 390
DriI GACNNNNNGTC 1 cut(s) 459
Eam1105I GACNNNNNGTC 1 cut(s) 459
EciI GGCGGA 1 cut(s) 404
Ecl136II GAGCTC 1 cut(s) 167
Eco130I CCWWGG 1 cut(s) 938
Eco24I GRGCYC 2 cut(s) 169, 355
Eco32I GATATC 1 cut(s) 403
Eco47I GGWCC 3 cut(s) 547, 553, 802
Eco53kI GAGCTC 1 cut(s) 167
Eco91I GGTNACC 1 cut(s) 1802
EcoICRI GAGCTC 1 cut(s) 167
EcoO109I RGGNCCY 4 cut(s) 239, 547, 802, 923
EcoO65I GGTNACC 1 cut(s) 1802
EcoRII CCWGG 3 cut(s) 418, 549, 1673
EcoRV GATATC 1 cut(s) 403
EcoT14I CCWWGG 1 cut(s) 938
EcoT38I GRGCYC 2 cut(s) 169, 355
ErhI CCWWGG 1 cut(s) 938
Esp3I CGTCTC 1 cut(s) 1787
FaeI CATG 8 cut(s) 251, 268, 313, 359, 656, 739, 895, 1049
FaqI GGGAC 1 cut(s) 560
FatI CATG 8 cut(s) 247, 264, 309, 355, 652, 735, 891, 1045
FauI CCCGC 1 cut(s) 1819
FbaI TGATCA 2 cut(s) 367, 609
Fnu4HI GCNGC 3 cut(s) 296, 1260, 1608
FokI GGATG 3 cut(s) 377, 617, 1065
FriOI GRGCYC 2 cut(s) 169, 355
Fsp4HI GCNGC 3 cut(s) 296, 1260, 1608
FspBI CTAG 3 cut(s) 999, 1632, 1743
GluI GCNGC 3 cut(s) 296, 1260, 1608
GsaI CCCAGC 1 cut(s) 1173
GsuI CTGGAG 1 cut(s) 520
HaeIII GGCC 3 cut(s) 241, 925, 1721
HapII CCGG 3 cut(s) 147, 290, 1781
HgaI GACGC 1 cut(s) 449
Hin1II CATG 8 cut(s) 251, 268, 313, 359, 656, 739, 895, 1049
HinfI GANTC 5 cut(s) 29, 411, 934, 1109, 1271
HpaII CCGG 3 cut(s) 147, 290, 1781
HphI GGTGA 7 cut(s) 169, 214, 410, 829, 907, 1814, 1832
Hpy166II GTNNAC 2 cut(s) 325, 898
Hpy188I TCNGA 9 cut(s) 171, 189, 307, 441, 465, 557, 601, 1018, 1092
Hpy188III TCNNGA 3 cut(s) 703, 814, 1046
Hpy8I GTNNAC 2 cut(s) 325, 898
HpyCH4III ACNGT 6 cut(s) 42, 827, 841, 1289, 1700, 1820
HpyCH4IV ACGT 2 cut(s) 507, 1810
HpyCH4V TGCA 5 cut(s) 482, 491, 656, 1023, 1262
HpyF10VI GCNNNNNNNGC 2 cut(s) 488, 1004
HpyF3I CTNAG 6 cut(s) 168, 470, 600, 948, 1089, 1362
HpySE526I ACGT 2 cut(s) 507, 1810
Hsp92II CATG 8 cut(s) 251, 268, 313, 359, 656, 739, 895, 1049
Ksp22I TGATCA 2 cut(s) 367, 609
Kzo9I GATC 7 cut(s) 367, 422, 609, 872, 951, 1013, 1246
LmnI GCTCC 7 cut(s) 527, 719, 831, 1396, 1417, 1618, 1786
Lsp1109I GCAGC 3 cut(s) 282, 1246, 1619
LweI GCATC 1 cut(s) 133
MabI ACCWGGT 1 cut(s) 549
MaeI CTAG 3 cut(s) 999, 1632, 1743
MaeII ACGT 2 cut(s) 507, 1810
MaeIII GTNAC 5 cut(s) 220, 821, 963, 1228, 1802
MalI GATC 7 cut(s) 369, 424, 611, 874, 953, 1015, 1248
MboI GATC 7 cut(s) 367, 422, 609, 872, 951, 1013, 1246
MflI RGATCY 1 cut(s) 422
MhlI GDGCHC 2 cut(s) 169, 355
MluCI AATT 9 cut(s) 346, 474, 625, 817, 1027, 1150, 1160, 1332, 1839
MlyI GAGTC 2 cut(s) 405, 1103
MmeI TCCRAC 2 cut(s) 809, 1305
MseI TTAA 3 cut(s) 389, 477, 743
MslI CAYNNNNRTG 1 cut(s) 263
MspI CCGG 3 cut(s) 147, 290, 1781
MspR9I CCNGG 4 cut(s) 148, 420, 551, 1675
Mva1269I GAATGC 1 cut(s) 1025
MvaI CCWGG 3 cut(s) 420, 551, 1675
MwoI GCNNNNNNNGC 2 cut(s) 488, 1004
NciI CCSGG 1 cut(s) 148
NdeII GATC 7 cut(s) 367, 422, 609, 872, 951, 1013, 1246
NheI GCTAGC 1 cut(s) 998
NlaIII CATG 8 cut(s) 251, 268, 313, 359, 656, 739, 895, 1049
NlaIV GGNNCC 7 cut(s) 270, 384, 548, 721, 804, 833, 924
NmuCI GTSAC 2 cut(s) 220, 1802
PagI TCATGA 1 cut(s) 1045
PctI GAATGC 1 cut(s) 1025
PfeI GAWTC 3 cut(s) 29, 934, 1271
PflFI GACNNNGTC 1 cut(s) 551
PflMI CCANNNNNTGG 1 cut(s) 1084
PkrI GCNGC 3 cut(s) 297, 1261, 1609
PleI GAGTC 2 cut(s) 405, 1103
PpsI GAGTC 2 cut(s) 405, 1103
PpuMI RGGWCCY 2 cut(s) 547, 802
PshBI ATTAAT 1 cut(s) 477
Psp124BI GAGCTC 1 cut(s) 169
Psp1406I AACGTT 1 cut(s) 1810
Psp5II RGGWCCY 2 cut(s) 547, 802
Psp6I CCWGG 3 cut(s) 418, 549, 1673
PspEI GGTNACC 1 cut(s) 1802
PspFI CCCAGC 1 cut(s) 1169
PspGI CCWGG 3 cut(s) 418, 549, 1673
PspN4I GGNNCC 7 cut(s) 270, 384, 548, 721, 804, 833, 924
PspPI GGNCC 6 cut(s) 239, 547, 553, 802, 923, 1720
PspPPI RGGWCCY 2 cut(s) 547, 802
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 422
PsyI GACNNNGTC 1 cut(s) 551
RsaI GTAC 4 cut(s) 197, 1068, 1291, 1697
RsaNI GTAC 4 cut(s) 196, 1067, 1290, 1696
RseI CAYNNNNRTG 1 cut(s) 263
SacI GAGCTC 1 cut(s) 169
SaqAI TTAA 3 cut(s) 389, 477, 743
SatI GCNGC 3 cut(s) 296, 1260, 1608
Sau3AI GATC 7 cut(s) 367, 422, 609, 872, 951, 1013, 1246
Sau96I GGNCC 6 cut(s) 239, 547, 553, 802, 923, 1720
SchI GAGTC 2 cut(s) 405, 1103
ScrFI CCNGG 4 cut(s) 148, 420, 551, 1675
SduI GDGCHC 2 cut(s) 169, 355
SexAI ACCWGGT 1 cut(s) 549
SfaNI GCATC 1 cut(s) 133
SinI GGWCC 3 cut(s) 547, 553, 802
SmiMI CAYNNNNRTG 1 cut(s) 263
Sse9I AATT 9 cut(s) 346, 474, 625, 817, 1027, 1150, 1160, 1332, 1839
SsiI CCGC 2 cut(s) 415, 1826
SspI AATATT 1 cut(s) 790
SspMI CTAG 3 cut(s) 999, 1632, 1743
SstI GAGCTC 1 cut(s) 169
StyD4I CCNGG 4 cut(s) 146, 418, 549, 1673
StyI CCWWGG 1 cut(s) 938
TaaI ACNGT 6 cut(s) 42, 827, 841, 1289, 1700, 1820
TaiI ACGT 2 cut(s) 510, 1813
TaqI TCGA 4 cut(s) 564, 617, 1245, 1284
TaqII GACCGA 2 cut(s) 470, 570
TasI AATT 9 cut(s) 346, 474, 625, 817, 1027, 1150, 1160, 1332, 1839
TatI WGTACW 2 cut(s) 1289, 1695
TfiI GAWTC 3 cut(s) 29, 934, 1271
Tru1I TTAA 3 cut(s) 389, 477, 743
Tru9I TTAA 3 cut(s) 389, 477, 743
TscAI CASTG 3 cut(s) 544, 663, 1221
TseFI GTSAC 2 cut(s) 220, 1802
TseI GCWGC 3 cut(s) 295, 1259, 1607
Tsp45I GTSAC 2 cut(s) 220, 1802
TspDTI ATGAA 6 cut(s) 49, 247, 852, 880, 1034, 1062
TspGWI ACGGA 4 cut(s) 213, 247, 1417, 1811
TspRI CASTG 3 cut(s) 544, 663, 1221
Tth111I GACNNNGTC 1 cut(s) 551
Van91I CCANNNNNTGG 1 cut(s) 1084
VpaK11BI GGWCC 3 cut(s) 547, 553, 802
VspI ATTAAT 1 cut(s) 477
XapI RAATTY 3 cut(s) 625, 1027, 1839
XcmI CCANNNNNNNNNTGG 1 cut(s) 332
XspI CTAG 3 cut(s) 999, 1632, 1743
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.