RchiOBHm_Chr2g0120631

GDP-fucose protein O-fucosyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0015813
Physical Location & Seq
Forward (+)
33419473 .. 33423345
3873 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 774 bp
ATGGTACAGAGAGGCTATCACAGACCCAGACCACCATCTCCACACGCCATTAAACTCAACCTAATCCTCATTTTTGCCTTCTTCTTTTTGGTCTTCATCTTCTACACAAGGCAATGGCATCTGCTCGACGATCAGCGAAAGCAACAGCCTTTCTCCGATGAACATTCCCAAACTGACCGGATGCCAGATGCAGAACTTTGGGATGCTCCTCCTAGCTATGGTTTACGCTCATGTGTCAAGCCGACCTCTAGATATAAAGCTGCCACTGGATGGGACCACTACATGACTGTGAGGAGTAATGGAGGACTAAATCAGATGCGAACAGGCATTGCAGACATGGTGGCTGTGGCACGCATTATGAATGCGACCTTGGTCATTCCTCAATTGGATAAACGTTCATTCTGGCATGACTCGAGTACATTTTCAGATATATTTGATGAACTTCACTTCATTGAAACTCTGCAAGAAGATGTCAAGATTGTCAAGGAGCTCCCACAGGAATTGGCAAATGTTCCTCGGGCGAGGAAGCATTTCACATCCTGGGCTAGCACCAGTTACTATGAAGACATGACACAGCTATGGAAAGAATATCAGGTGATTCATGTTGCAAAATCAGATTCTCGACTTGCAAACAATGATCTTCCTCTTGACATCCAGAGGTTGAGATGCCGTGCCTTGTATCATGCGCTCCGTTTCTCTCCCCCAATTGAGAGCTTTGGAAAGGTTCTGAGCATGCATGACGCTGACACCGCAGAAGCTGGTGGAGCGGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

257

Amino Acids

29.74

Weight (kDa)

7.19

Isoelectric Point (pI)

54.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 94 - 243 7.1e-49 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015813)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G64600
fragaria_vesca FvH4_1g24200 FvH4_1g24200 FvH4_1g24200
rosa_chinensis RchiOBHm_Chr2g0120631 RchiOBHm_Chr2g0120641
rosa_laevigata RLG00000018563
rosa_multiflora Rmu_sc0001844.1_g000005
rosa_roxburghii Rroxscaffold_2G00123270
rosa_rugosa Rorug02G0230000
rosa_samantha Rh2AG286000 Rh2BG297800 Rh2CG275500 Rh2DG311500
rosa_wichuraiana Rw2G022970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 270
AccBSI CCGCTC 1 cut(s) 767
AciI CCGC 2 cut(s) 750, 767
AclI AACGTT 1 cut(s) 394
AfaI GTAC 2 cut(s) 6, 418
AfiI CCNNNNNNNGG 2 cut(s) 218, 270
AgsI TTSAA 1 cut(s) 455
AjnI CCWGG 1 cut(s) 539
AjuI GAANNNNNNNTTGG 2 cut(s) 353, 385
AluBI AGCT 6 cut(s) 216, 260, 490, 577, 714, 758
AluI AGCT 6 cut(s) 216, 260, 490, 577, 714, 758
Alw21I GWGCWC 1 cut(s) 492
AlwNI CAGNNNCTG 1 cut(s) 758
Ama87I CYCGRG 2 cut(s) 412, 516
ApeKI GCWGC 1 cut(s) 260
Asp700I GAANNNNTTC 1 cut(s) 530
AspLEI GCGC 1 cut(s) 688
AspS9I GGNCC 1 cut(s) 274
AsuHPI GGTGA 1 cut(s) 607
AsuNHI GCTAGC 1 cut(s) 545
AvaI CYCGRG 2 cut(s) 412, 516
AvaII GGWCC 1 cut(s) 274
BanII GRGCYC 1 cut(s) 492
BbsI GAAGAC 2 cut(s) 85, 570
Bbv12I GWGCWC 1 cut(s) 492
BbvI GCAGC 1 cut(s) 247
BccI CCATC 2 cut(s) 43, 264
BceAI ACGGC 1 cut(s) 654
BciT130I CCWGG 1 cut(s) 541
BfaI CTAG 3 cut(s) 213, 249, 546
BisI GCNGC 2 cut(s) 261, 768
BlsI GCNGC 2 cut(s) 262, 769
Bme1390I CCNGG 1 cut(s) 541
Bme18I GGWCC 1 cut(s) 274
BmeT110I CYCGRG 2 cut(s) 412, 516
BmgT120I GGNCC 1 cut(s) 274
BmiI GGNNCC 1 cut(s) 275
BmrFI CCNGG 1 cut(s) 541
BmsI GCATC 6 cut(s) 127, 171, 178, 193, 306, 656
BmtI GCTAGC 1 cut(s) 549
BoxI GACNNNNGTC 1 cut(s) 371
BpiI GAAGAC 2 cut(s) 85, 570
BsaJI CCNNGG 3 cut(s) 369, 515, 540
BsaWI WCCGGW 1 cut(s) 177
Bsc4I CCNNNNNNNGG 2 cut(s) 218, 270
Bse1I ACTGG 2 cut(s) 271, 552
Bse3DI GCAATG 2 cut(s) 119, 327
BseBI CCWGG 1 cut(s) 541
BseDI CCNNGG 3 cut(s) 369, 515, 540
BseGI GGATG 5 cut(s) 186, 208, 275, 536, 651
BseLI CCNNNNNNNGG 2 cut(s) 218, 270
BseMI GCAATG 2 cut(s) 119, 327
BseMII CTCAG 1 cut(s) 719
BseNI ACTGG 2 cut(s) 271, 552
BseRI GAGGAG 2 cut(s) 198, 307
BseXI GCAGC 1 cut(s) 247
BsiHKAI GWGCWC 1 cut(s) 492
BsiHKCI CYCGRG 2 cut(s) 412, 516
BsiSI CCGG 1 cut(s) 178
BslFI GGGAC 1 cut(s) 287
BslI CCNNNNNNNGG 2 cut(s) 218, 270
BsmFI GGGAC 1 cut(s) 287
BsmI GAATGC 1 cut(s) 367
BsoBI CYCGRG 2 cut(s) 412, 516
Bsp1286I GDGCHC 1 cut(s) 492
Bsp143I GATC 2 cut(s) 130, 637
BspACI CCGC 2 cut(s) 750, 767
BspCNI CTCAG 1 cut(s) 720
BspLI GGNNCC 1 cut(s) 275
BspOI GCTAGC 1 cut(s) 549
BsrBI CCGCTC 1 cut(s) 767
BsrDI GCAATG 2 cut(s) 119, 327
BsrI ACTGG 2 cut(s) 271, 552
BssECI CCNNGG 3 cut(s) 369, 515, 540
BssMI GATC 2 cut(s) 130, 637
BssT1I CCWWGG 1 cut(s) 369
Bst2UI CCWGG 1 cut(s) 541
Bst4CI ACNGT 1 cut(s) 289
BstC8I GCNNGC 3 cut(s) 352, 547, 734
BstDEI CTNAG 2 cut(s) 728, 771
BstF5I GGATG 5 cut(s) 186, 208, 275, 536, 651
BstHHI GCGC 1 cut(s) 688
BstKTI GATC 2 cut(s) 133, 640
BstMBI GATC 2 cut(s) 130, 637
BstMWI GCNNNNNNNGC 2 cut(s) 749, 764
BstNI CCWGG 1 cut(s) 541
BstNSI RCATGY 1 cut(s) 736
BstPAI GACNNNNGTC 1 cut(s) 371
BstSCI CCNGG 1 cut(s) 539
BstV1I GCAGC 1 cut(s) 247
BstV2I GAAGAC 2 cut(s) 85, 570
BtsCI GGATG 5 cut(s) 186, 208, 275, 536, 651
BtsIMutI CAGTG 1 cut(s) 264
Cac8I GCNNGC 3 cut(s) 352, 547, 734
CaiI CAGNNNCTG 1 cut(s) 758
CfoI GCGC 1 cut(s) 688
Cfr13I GGNCC 1 cut(s) 274
CseI GACGC 1 cut(s) 749
Csp6I GTAC 2 cut(s) 5, 417
CspCI CAANNNNNGTGG 2 cut(s) 483, 518
CviAII CATG 9 cut(s) 231, 283, 337, 407, 568, 602, 683, 733, 737
CviQI GTAC 2 cut(s) 5, 417
DdeI CTNAG 2 cut(s) 728, 771
DpnI GATC 2 cut(s) 132, 639
DpnII GATC 2 cut(s) 130, 637
Ecl136II GAGCTC 1 cut(s) 490
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 1 cut(s) 492
Eco47I GGWCC 1 cut(s) 274
Eco53kI GAGCTC 1 cut(s) 490
Eco88I CYCGRG 2 cut(s) 412, 516
EcoICRI GAGCTC 1 cut(s) 490
EcoRII CCWGG 1 cut(s) 539
EcoT14I CCWWGG 1 cut(s) 369
EcoT22I ATGCAT 1 cut(s) 738
EcoT38I GRGCYC 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 9 cut(s) 234, 286, 340, 410, 571, 605, 686, 736, 740
FaqI GGGAC 1 cut(s) 287
FatI CATG 9 cut(s) 230, 282, 336, 406, 567, 601, 682, 732, 736
Fnu4HI GCNGC 2 cut(s) 261, 768
FokI GGATG 5 cut(s) 193, 215, 282, 523, 638
FriOI GRGCYC 1 cut(s) 492
Fsp4HI GCNGC 2 cut(s) 261, 768
FspBI CTAG 3 cut(s) 213, 249, 546
GlaI GCGC 1 cut(s) 687
GluI GCNGC 2 cut(s) 261, 768
HapII CCGG 1 cut(s) 178
HgaI GACGC 1 cut(s) 749
HhaI GCGC 1 cut(s) 688
Hin1II CATG 9 cut(s) 234, 286, 340, 410, 571, 605, 686, 736, 740
Hin6I GCGC 1 cut(s) 686
HinP1I GCGC 1 cut(s) 686
HinfI GANTC 3 cut(s) 410, 598, 617
HpaII CCGG 1 cut(s) 178
HphI GGTGA 1 cut(s) 607
Hpy166II GTNNAC 1 cut(s) 224
Hpy188I TCNGA 5 cut(s) 157, 315, 427, 616, 729
Hpy188III TCNNGA 5 cut(s) 249, 475, 621, 647, 655
Hpy8I GTNNAC 1 cut(s) 224
Hpy99I CGWCG 1 cut(s) 131
HpyAV CCTTC 1 cut(s) 88
HpyCH4III ACNGT 1 cut(s) 289
HpyCH4IV ACGT 1 cut(s) 394
HpyCH4V TGCA 6 cut(s) 191, 332, 463, 608, 629, 736
HpyF10VI GCNNNNNNNGC 2 cut(s) 749, 764
HpyF3I CTNAG 2 cut(s) 728, 771
HpySE526I ACGT 1 cut(s) 394
Hsp92II CATG 9 cut(s) 234, 286, 340, 410, 571, 605, 686, 736, 740
HspAI GCGC 1 cut(s) 686
Kzo9I GATC 2 cut(s) 130, 637
LmnI GCTCC 5 cut(s) 211, 487, 495, 693, 764
Lsp1109I GCAGC 1 cut(s) 247
LweI GCATC 6 cut(s) 127, 171, 178, 193, 306, 656
MaeI CTAG 3 cut(s) 213, 249, 546
MaeII ACGT 1 cut(s) 394
MaeIII GTNAC 1 cut(s) 554
MalI GATC 2 cut(s) 132, 639
MbiI CCGCTC 1 cut(s) 767
MboI GATC 2 cut(s) 130, 637
MboII GAAGA 6 cut(s) 73, 85, 91, 479, 575, 632
MfeI CAATTG 2 cut(s) 383, 705
MhlI GDGCHC 1 cut(s) 492
MluCI AATT 3 cut(s) 383, 500, 705
MlyI GAGTC 1 cut(s) 404
Mph1103I ATGCAT 1 cut(s) 738
MroXI GAANNNNTTC 1 cut(s) 530
MseI TTAA 1 cut(s) 51
MslI CAYNNNNRTG 2 cut(s) 287, 577
MspI CCGG 1 cut(s) 178
MspR9I CCNGG 1 cut(s) 541
MunI CAATTG 2 cut(s) 383, 705
Mva1269I GAATGC 1 cut(s) 367
MvaI CCWGG 1 cut(s) 541
MwoI GCNNNNNNNGC 2 cut(s) 749, 764
NdeII GATC 2 cut(s) 130, 637
NheI GCTAGC 1 cut(s) 545
NlaIII CATG 9 cut(s) 234, 286, 340, 410, 571, 605, 686, 736, 740
NlaIV GGNNCC 1 cut(s) 275
NsiI ATGCAT 1 cut(s) 738
NspI RCATGY 1 cut(s) 736
PaeI GCATGC 1 cut(s) 736
PaeR7I CTCGAG 1 cut(s) 412
PctI GAATGC 1 cut(s) 367
PdmI GAANNNNTTC 1 cut(s) 530
PfeI GAWTC 2 cut(s) 598, 617
PflMI CCANNNNNTGG 1 cut(s) 270
PkrI GCNGC 2 cut(s) 262, 769
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PshAI GACNNNNGTC 1 cut(s) 371
Psp124BI GAGCTC 1 cut(s) 492
Psp1406I AACGTT 1 cut(s) 394
Psp6I CCWGG 1 cut(s) 539
PspGI CCWGG 1 cut(s) 539
PspN4I GGNNCC 1 cut(s) 275
PspPI GGNCC 1 cut(s) 274
PspXI VCTCGAGB 1 cut(s) 412
PstNI CAGNNNCTG 1 cut(s) 758
RsaI GTAC 2 cut(s) 6, 418
RsaNI GTAC 2 cut(s) 5, 417
RseI CAYNNNNRTG 2 cut(s) 287, 577
SacI GAGCTC 1 cut(s) 492
SaqAI TTAA 1 cut(s) 51
SatI GCNGC 2 cut(s) 261, 768
Sau3AI GATC 2 cut(s) 130, 637
Sau96I GGNCC 1 cut(s) 274
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 541
SduI GDGCHC 1 cut(s) 492
SfaNI GCATC 6 cut(s) 127, 171, 178, 193, 306, 656
Sfr274I CTCGAG 1 cut(s) 412
SinI GGWCC 1 cut(s) 274
SlaI CTCGAG 1 cut(s) 412
SmiMI CAYNNNNRTG 2 cut(s) 287, 577
SmlI CTYRAG 1 cut(s) 412
SmoI CTYRAG 1 cut(s) 412
SphI GCATGC 1 cut(s) 736
Sse9I AATT 3 cut(s) 383, 500, 705
SsiI CCGC 2 cut(s) 750, 767
SspMI CTAG 3 cut(s) 213, 249, 546
SstI GAGCTC 1 cut(s) 492
StyD4I CCNGG 1 cut(s) 539
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 1 cut(s) 289
TaiI ACGT 1 cut(s) 397
TaqI TCGA 3 cut(s) 126, 413, 622
TasI AATT 3 cut(s) 383, 500, 705
TatI WGTACW 1 cut(s) 416
TauI GCSGC 1 cut(s) 770
TfiI GAWTC 2 cut(s) 598, 617
Tru1I TTAA 1 cut(s) 51
Tru9I TTAA 1 cut(s) 51
TscAI CASTG 1 cut(s) 271
TseI GCWGC 1 cut(s) 260
TspDTI ATGAA 8 cut(s) 85, 174, 374, 387, 439, 453, 576, 590
TspGWI ACGGA 1 cut(s) 680
TspRI CASTG 1 cut(s) 271
Van91I CCANNNNNTGG 1 cut(s) 270
VpaK11BI GGWCC 1 cut(s) 274
XbaI TCTAGA 1 cut(s) 248
XceI RCATGY 1 cut(s) 736
XhoI CTCGAG 1 cut(s) 412
XmnI GAANNNNTTC 1 cut(s) 530
XspI CTAG 3 cut(s) 213, 249, 546
Zsp2I ATGCAT 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.