RchiOBHm_Chr2g0140491

Sigma factor PP2C-like phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0018325
Physical Location & Seq
Forward (+)
57953297 .. 57955223
1927 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGGGAGCCCTTGTTAGGGCTTACAACAGAGCAGATGATGCTTTTATAGACGAGACATTAGCACCAGATACAGTTGGATCGACAGCTGTGGTAGTGATTCTGTCAGCCTGTCAGATCATTGTTGCCAATTGCGGTGACTCTAGGGCTGTGCTTTGTCGTGGAAATCAAGTGATCCCTTTGACTGTCGACCACAAGCTTGACAGAGACGATGAAGTAATTACAGTTACTGAAGCAGGGGGCAGGATTCTGTATGTTGGATGCCCTACAGTTGAGGGGGTTCTCGCTATGACAAGAGCAATAGGTTTGCTTTACCCTTACACTCCTTTTCCCTTTTTGTACTCATTGCTTGTGGGAAATATTATCAACAAGCAAACTGATACTGATGAATGCTTGATTCTGGCGAGTGATGGCTTATGGGATGTGATGTCTAATGAAAAGGTGATGGCGATGGCTCGCAGGATGCTGAGGGAGCTTCGCAATCAGTATGCAACGGTAGCCAATGGATCAGTTAGCCCAGCACAGTATGTTGCAGAAGAAGTCCTCAAGAATGCTCTTCGAACTAGTACTGACAACGTTTCCATTATTGTTGTTGACCTGAAAAGGCTGAGGAGAAGGATGCAACAAAAGGCAGAAGCCGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

214

Amino Acids

23.46

Weight (kDa)

5.11

Isoelectric Point (pI)

42.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 10 - 183 1.7e-36 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17545
rosa_chinensis RchiOBHm_Chr2g0140491
rosa_laevigata RLG00000019871
rosa_rugosa Rorug02G0363300
rosa_samantha Rh2AG413800 Rh2AG413900 Rh2BG424000 Rh2CG400200 Rh2CG400300 Rh2DG433700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 186
AciI CCGC 1 cut(s) 132
AclI AACGTT 1 cut(s) 573
AclWI GGATC 3 cut(s) 85, 166, 511
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 2 cut(s) 338, 565
AfiI CCNNNNNNNGG 2 cut(s) 15, 16
AhlI ACTAGT 1 cut(s) 560
AluBI AGCT 3 cut(s) 86, 196, 472
AluI AGCT 3 cut(s) 86, 196, 472
Alw26I GTCTC 2 cut(s) 47, 198
AlwI GGATC 3 cut(s) 85, 166, 511
AlwNI CAGNNNCTG 1 cut(s) 227
AsuHPI GGTGA 2 cut(s) 146, 451
AsuII TTCGAA 1 cut(s) 556
BanII GRGCYC 1 cut(s) 10
BbvCI CCTCAGC 2 cut(s) 464, 605
BccI CCATC 3 cut(s) 401, 436, 442
BcoDI GTCTC 2 cut(s) 47, 198
BcuI ACTAGT 1 cut(s) 560
BfaI CTAG 2 cut(s) 141, 561
BfmI CTRYAG 1 cut(s) 264
BmcAI AGTACT 1 cut(s) 565
BmiI GGNNCC 1 cut(s) 7
BmsI GCATC 4 cut(s) 28, 248, 450, 606
Bpu10I CCTNAGC 2 cut(s) 464, 605
Bpu14I TTCGAA 1 cut(s) 556
BpuEI CTTGAG 1 cut(s) 527
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 16
Bse3DI GCAATG 1 cut(s) 341
BseGI GGATG 4 cut(s) 263, 424, 465, 621
BseLI CCNNNNNNNGG 2 cut(s) 15, 16
BseMI GCAATG 1 cut(s) 341
BseMII CTCAG 2 cut(s) 455, 596
BseRI GAGGAG 1 cut(s) 622
BseYI CCCAGC 1 cut(s) 514
BslI CCNNNNNNNGG 2 cut(s) 15, 16
BsmAI GTCTC 2 cut(s) 47, 198
BsmBI CGTCTC 1 cut(s) 198
BsmI GAATGC 2 cut(s) 392, 553
Bsp119I TTCGAA 1 cut(s) 556
Bsp1286I GDGCHC 1 cut(s) 10
Bsp143I GATC 4 cut(s) 77, 114, 171, 503
BspACI CCGC 1 cut(s) 132
BspCNI CTCAG 2 cut(s) 456, 597
BspLI GGNNCC 1 cut(s) 7
BspPI GGATC 3 cut(s) 85, 166, 511
BspQI GCTCTTC 1 cut(s) 558
BspT104I TTCGAA 1 cut(s) 556
BsrDI GCAATG 1 cut(s) 341
BssMI GATC 4 cut(s) 77, 114, 171, 503
Bst4CI ACNGT 6 cut(s) 73, 184, 223, 268, 493, 522
Bst6I CTCTTC 1 cut(s) 558
BstAPI GCANNNNNTGC 1 cut(s) 38
BstBI TTCGAA 1 cut(s) 556
BstC8I GCNNGC 1 cut(s) 454
BstDEI CTNAG 2 cut(s) 464, 605
BstF5I GGATG 4 cut(s) 263, 424, 465, 621
BstKTI GATC 4 cut(s) 80, 117, 174, 506
BstMAI GTCTC 2 cut(s) 47, 198
BstMBI GATC 4 cut(s) 77, 114, 171, 503
BstMWI GCNNNNNNNGC 3 cut(s) 38, 469, 494
BstSFI CTRYAG 1 cut(s) 264
BtgZI GCGATG 1 cut(s) 461
BtsCI GGATG 4 cut(s) 263, 424, 465, 621
Cac8I GCNNGC 1 cut(s) 454
CaiI CAGNNNCTG 1 cut(s) 227
Csp6I GTAC 2 cut(s) 337, 564
CviQI GTAC 2 cut(s) 337, 564
DdeI CTNAG 2 cut(s) 464, 605
DpnI GATC 4 cut(s) 79, 116, 173, 505
DpnII GATC 4 cut(s) 77, 114, 171, 503
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
Eco24I GRGCYC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 249
EcoT38I GRGCYC 1 cut(s) 10
Esp3I CGTCTC 1 cut(s) 198
FaiI YATR 8 cut(s) 47, 252, 287, 415, 486, 525, 641, 643
FblI GTMKAC 1 cut(s) 186
FokI GGATG 4 cut(s) 270, 431, 472, 628
FriOI GRGCYC 1 cut(s) 10
FspBI CTAG 2 cut(s) 141, 561
GsaI CCCAGC 1 cut(s) 518
HincII GTYRAC 2 cut(s) 187, 592
HindII GTYRAC 2 cut(s) 187, 592
HindIII AAGCTT 1 cut(s) 194
HinfI GANTC 4 cut(s) 97, 137, 244, 394
HphI GGTGA 2 cut(s) 146, 451
Hpy166II GTNNAC 2 cut(s) 187, 592
Hpy188I TCNGA 1 cut(s) 114
Hpy188III TCNNGA 1 cut(s) 544
Hpy8I GTNNAC 2 cut(s) 187, 592
HpyAV CCTTC 1 cut(s) 606
HpyCH4III ACNGT 6 cut(s) 73, 184, 223, 268, 493, 522
HpyCH4IV ACGT 1 cut(s) 573
HpyCH4V TGCA 3 cut(s) 488, 530, 619
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 469, 494
HpyF3I CTNAG 2 cut(s) 464, 605
HpySE526I ACGT 1 cut(s) 573
Kzo9I GATC 4 cut(s) 77, 114, 171, 503
LguI GCTCTTC 1 cut(s) 558
LmnI GCTCC 2 cut(s) 5, 469
LpnPI CCDG 8 cut(s) 78, 121, 219, 226, 383, 442, 528, 608
LweI GCATC 4 cut(s) 28, 248, 450, 606
MaeI CTAG 2 cut(s) 141, 561
MaeII ACGT 1 cut(s) 573
MaeIII GTNAC 2 cut(s) 134, 223
MalI GATC 4 cut(s) 79, 116, 173, 505
MboI GATC 4 cut(s) 77, 114, 171, 503
MboII GAAGA 2 cut(s) 545, 545
MfeI CAATTG 1 cut(s) 127
MhlI GDGCHC 1 cut(s) 10
MluCI AATT 2 cut(s) 127, 216
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 2 cut(s) 55, 235
MnlI CCTC 4 cut(s) 265, 459, 551, 600
MspA1I CMGCKG 1 cut(s) 86
MunI CAATTG 1 cut(s) 127
Mva1269I GAATGC 2 cut(s) 392, 553
MwoI GCNNNNNNNGC 3 cut(s) 38, 469, 494
NdeII GATC 4 cut(s) 77, 114, 171, 503
NlaIV GGNNCC 1 cut(s) 7
NmuCI GTSAC 1 cut(s) 134
NspV TTCGAA 1 cut(s) 556
PciSI GCTCTTC 1 cut(s) 558
PctI GAATGC 2 cut(s) 392, 553
PfeI GAWTC 3 cut(s) 97, 244, 394
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
Psp1406I AACGTT 1 cut(s) 573
PspFI CCCAGC 1 cut(s) 514
PspN4I GGNNCC 1 cut(s) 7
PstNI CAGNNNCTG 1 cut(s) 227
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 2 cut(s) 338, 565
RsaNI GTAC 2 cut(s) 337, 564
SalI GTCGAC 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 558
Sau3AI GATC 4 cut(s) 77, 114, 171, 503
ScaI AGTACT 1 cut(s) 565
SchI GAGTC 1 cut(s) 131
SduI GDGCHC 1 cut(s) 10
SetI ASST 7 cut(s) 88, 198, 304, 441, 474, 576, 597
SfaNI GCATC 4 cut(s) 28, 248, 450, 606
SfcI CTRYAG 1 cut(s) 264
SfuI TTCGAA 1 cut(s) 556
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
SpeI ACTAGT 1 cut(s) 560
Sse9I AATT 2 cut(s) 127, 216
SsiI CCGC 1 cut(s) 132
SspI AATATT 1 cut(s) 358
SspMI CTAG 2 cut(s) 141, 561
TaaI ACNGT 6 cut(s) 73, 184, 223, 268, 493, 522
TaiI ACGT 1 cut(s) 576
TaqI TCGA 3 cut(s) 80, 186, 556
TasI AATT 2 cut(s) 127, 216
TatI WGTACW 2 cut(s) 336, 563
TfiI GAWTC 3 cut(s) 97, 244, 394
TseFI GTSAC 1 cut(s) 134
Tsp45I GTSAC 1 cut(s) 134
TspDTI ATGAA 3 cut(s) 225, 399, 447
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 2 cut(s) 141, 561
ZrmI AGTACT 1 cut(s) 565
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.