Rh2BG424000

Sigma factor PP2C-like phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0018325
Physical Location & Seq
Forward (+)
59700277 .. 59736171
35895 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG424000.1

Sequence Viewer

Length: 1089 bp
ATGAATCCGGTGGCCAATATGAGGCTTGATGATGAAGAAGCTGCTTCACCAAAGAGTGAAGGTGCAAGCACTGAACCAGAACCCAAAGCCGTCGCGGCGGCCACTGAGTCCTCTGAAAGCAGCGGCGGCAGAAGCAATGGCGGCGAGGTGGTTTTTGTGGGGAGGAAGAACAGGGCCGTGTCGTGGGGTTACAGCTTGAACATAGGAAGGAGACGAGAAATGGAAGACACCCTTACCATTGTTCCTTCTTTTATGCAAATTCCCTGCGCTTCTGTTGGCGGGTGTACAGCGCCGGAGTGTAGGTACGTCACGGAGGAGTCGTCGGTTCACTACTTCGGTTTGTTTGATGGCCACGGCGGCTCTGAGGTGAGTCGTTATTGCTCCAAAAGCCTCCATGAACTGTTGGCAGAGGAGTGGGAAAAAGGAAGCGGTGAAGATCAGTGGAATAAAAGGTGGATGGGAGCCCTTGTTAGGGCTTACAACAGAGCAGATGATGCTTTTATAGATGAGACATTAGCACCAGATACAGTTAGATCGACAGCTGTGGTAGTGATTCTGTCAGCCTGTCAGATCATTGTTGCCAATTGCGGTGACTCTAGGGCTGTGCTTTGTCGTGGAAATCAAGTGATCCCTTTGACTGTCGACCACAAGCCTGACAGAGACGATGAAGTAAGTAGAGTTACTGAAGCAGGGGGCAGGATTCTGTATGTTGGATGCCCTACAGTTGAGGGGGTTCTCGCTATGACAAGAGCAATAGGTGACCAAGATCTAAAGCCGTGGATTATTTCAGAGCCAGAAGTTACTTTTTTGACCAGAACTGATACTGATGAATGCTTGATTCTGGCGAGTGATGGCTTATGGGATGTGATGTCTAATGAAAAGGTGATGGCGATGGCTCGCAGGATGCTGAGGGAGCTTCGCAATCAGTATGCAACGGTAGCCAATGGATCAGTCAGCCCAGCACAGTATGTTGCAGAAGAAGTCCTCAAGAATGCTCTTCGAACTAGTACTGACAACGTTTCCATTATTGTTGTTGACCTGAAAAGGCTGAGGAGAAGGATGCAACAAAAGGCAGAAGCCGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

362

Amino Acids

39.71

Weight (kDa)

4.99

Isoelectric Point (pI)

54.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 69 - 328 4.3e-57 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17545
rosa_chinensis RchiOBHm_Chr2g0140491
rosa_laevigata RLG00000019871
rosa_rugosa Rorug02G0363300
rosa_samantha Rh2AG413800 Rh2AG413900 Rh2BG424000 Rh2CG400200 Rh2CG400300 Rh2DG433700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 642
AccII CGCG 1 cut(s) 95
AciI CCGC 9 cut(s) 95, 98, 123, 126, 141, 279, 357, 429, 588
AclI AACGTT 1 cut(s) 1017
AclWI GGATC 2 cut(s) 622, 955
AcoI YGGCCR 3 cut(s) 12, 99, 349
AcsI RAATTY 1 cut(s) 258
AcuI CTGAAG 1 cut(s) 705
AfaI GTAC 3 cut(s) 286, 305, 1009
AfiI CCNNNNNNNGG 4 cut(s) 21, 183, 471, 472
AgsI TTSAA 1 cut(s) 199
AhlI ACTAGT 1 cut(s) 1004
AluBI AGCT 4 cut(s) 41, 195, 542, 916
AluI AGCT 4 cut(s) 41, 195, 542, 916
Alw26I GTCTC 3 cut(s) 205, 503, 654
AlwI GGATC 2 cut(s) 622, 955
AoxI GGCC 4 cut(s) 12, 99, 174, 349
ApeKI GCWGC 2 cut(s) 41, 120
ApoI RAATTY 1 cut(s) 258
AspLEI GCGC 2 cut(s) 269, 292
AspS9I GGNCC 1 cut(s) 174
AsuHPI GGTGA 6 cut(s) 39, 379, 443, 602, 770, 895
AsuII TTCGAA 1 cut(s) 1000
BalI TGGCCA 2 cut(s) 14, 351
BanII GRGCYC 1 cut(s) 466
BbsI GAAGAC 1 cut(s) 231
BbvCI CCTCAGC 2 cut(s) 908, 1049
BbvI GCAGC 2 cut(s) 28, 132
BccI CCATC 5 cut(s) 341, 451, 845, 880, 886
BceAI ACGGC 4 cut(s) 74, 161, 370, 760
BcoDI GTCTC 3 cut(s) 205, 503, 654
BcuI ACTAGT 1 cut(s) 1004
BfaI CTAG 2 cut(s) 597, 1005
BfmI CTRYAG 1 cut(s) 720
BfoI RGCGCY 1 cut(s) 293
BglI GCCNNNNNGGC 2 cut(s) 95, 357
BglII AGATCT 1 cut(s) 766
BisI GCNGC 8 cut(s) 42, 96, 99, 121, 124, 127, 142, 358
BlsI GCNGC 8 cut(s) 43, 97, 100, 122, 125, 128, 143, 359
BmcAI AGTACT 1 cut(s) 1009
BmgT120I GGNCC 1 cut(s) 174
BmiI GGNNCC 1 cut(s) 463
BmsI GCATC 4 cut(s) 484, 704, 894, 1050
BpiI GAAGAC 1 cut(s) 231
Bpu10I CCTNAGC 2 cut(s) 908, 1049
Bpu14I TTCGAA 1 cut(s) 1000
BpuEI CTTGAG 1 cut(s) 971
BsaJI CCNNGG 2 cut(s) 352, 776
BsaWI WCCGGW 1 cut(s) 7
BsaXI ACNNNNNCTCC 6 cut(s) 287, 305, 317, 335, 453, 483
Bsc4I CCNNNNNNNGG 4 cut(s) 21, 183, 471, 472
Bse3DI GCAATG 1 cut(s) 142
BseDI CCNNGG 2 cut(s) 352, 776
BseGI GGATG 5 cut(s) 462, 719, 868, 909, 1065
BseLI CCNNNNNNNGG 4 cut(s) 21, 183, 471, 472
BseMI GCAATG 1 cut(s) 142
BseMII CTCAG 4 cut(s) 96, 354, 899, 1040
BseRI GAGGAG 3 cut(s) 329, 425, 1066
BseXI GCAGC 2 cut(s) 28, 132
BseYI CCCAGC 1 cut(s) 958
Bsh1236I CGCG 1 cut(s) 95
BshFI GGCC 4 cut(s) 14, 101, 176, 351
BsiSI CCGG 2 cut(s) 8, 293
BslI CCNNNNNNNGG 4 cut(s) 21, 183, 471, 472
BsmAI GTCTC 3 cut(s) 205, 503, 654
BsmBI CGTCTC 2 cut(s) 205, 654
BsmI GAATGC 2 cut(s) 836, 997
BsnI GGCC 4 cut(s) 14, 101, 176, 351
Bsp119I TTCGAA 1 cut(s) 1000
Bsp1286I GDGCHC 1 cut(s) 466
Bsp1407I TGTACA 1 cut(s) 284
Bsp143I GATC 6 cut(s) 436, 533, 570, 627, 766, 947
BspACI CCGC 9 cut(s) 95, 98, 123, 126, 141, 279, 357, 429, 588
BspANI GGCC 4 cut(s) 14, 101, 176, 351
BspCNI CTCAG 4 cut(s) 97, 355, 900, 1041
BspFNI CGCG 1 cut(s) 95
BspLI GGNNCC 1 cut(s) 463
BspPI GGATC 2 cut(s) 622, 955
BspQI GCTCTTC 1 cut(s) 1002
BspT104I TTCGAA 1 cut(s) 1000
BsrDI GCAATG 1 cut(s) 142
BsrGI TGTACA 1 cut(s) 284
BssECI CCNNGG 2 cut(s) 352, 776
BssMI GATC 6 cut(s) 436, 533, 570, 627, 766, 947
Bst4CI ACNGT 6 cut(s) 402, 529, 640, 724, 937, 966
Bst6I CTCTTC 1 cut(s) 1002
BstAPI GCANNNNNTGC 1 cut(s) 494
BstAUI TGTACA 1 cut(s) 284
BstBI TTCGAA 1 cut(s) 1000
BstC8I GCNNGC 2 cut(s) 67, 898
BstDEI CTNAG 4 cut(s) 105, 363, 908, 1049
BstDSI CCRYGG 2 cut(s) 352, 776
BstEII GGTNACC 1 cut(s) 758
BstF5I GGATG 5 cut(s) 462, 719, 868, 909, 1065
BstFNI CGCG 1 cut(s) 95
BstH2I RGCGCY 1 cut(s) 293
BstHHI GCGC 2 cut(s) 269, 292
BstKTI GATC 6 cut(s) 439, 536, 573, 630, 769, 950
BstMAI GTCTC 3 cut(s) 205, 503, 654
BstMBI GATC 6 cut(s) 436, 533, 570, 627, 766, 947
BstMWI GCNNNNNNNGC 9 cut(s) 95, 126, 132, 141, 357, 387, 494, 913, 938
BstPI GGTNACC 1 cut(s) 758
BstSFI CTRYAG 1 cut(s) 720
BstUI CGCG 1 cut(s) 95
BstV1I GCAGC 2 cut(s) 28, 132
BstV2I GAAGAC 1 cut(s) 231
BstX2I RGATCY 1 cut(s) 766
BstYI RGATCY 1 cut(s) 766
BsuRI GGCC 4 cut(s) 14, 101, 176, 351
BtgI CCRYGG 2 cut(s) 352, 776
BtgZI GCGATG 1 cut(s) 905
BtsCI GGATG 5 cut(s) 462, 719, 868, 909, 1065
BtsIMutI CAGTG 3 cut(s) 69, 102, 446
Cac8I GCNNGC 2 cut(s) 67, 898
CfoI GCGC 2 cut(s) 269, 292
Cfr13I GGNCC 1 cut(s) 174
Csp6I GTAC 3 cut(s) 285, 304, 1008
CviAII CATG 1 cut(s) 395
CviQI GTAC 3 cut(s) 285, 304, 1008
DdeI CTNAG 4 cut(s) 105, 363, 908, 1049
DpnI GATC 6 cut(s) 438, 535, 572, 629, 768, 949
DpnII GATC 6 cut(s) 436, 533, 570, 627, 766, 947
EaeI YGGCCR 3 cut(s) 12, 99, 349
Eam1104I CTCTTC 1 cut(s) 1002
EarI CTCTTC 1 cut(s) 1002
Eco24I GRGCYC 1 cut(s) 466
Eco57I CTGAAG 1 cut(s) 705
Eco91I GGTNACC 1 cut(s) 758
EcoO65I GGTNACC 1 cut(s) 758
EcoT38I GRGCYC 1 cut(s) 466
Esp3I CGTCTC 2 cut(s) 205, 654
FaeI CATG 1 cut(s) 398
FalI AAGNNNNNCTT 2 cut(s) 216, 248
FatI CATG 1 cut(s) 394
FauI CCCGC 1 cut(s) 272
FblI GTMKAC 1 cut(s) 642
Fnu4HI GCNGC 8 cut(s) 42, 96, 99, 121, 124, 127, 142, 358
FokI GGATG 5 cut(s) 469, 726, 875, 916, 1072
FriOI GRGCYC 1 cut(s) 466
Fsp4HI GCNGC 8 cut(s) 42, 96, 99, 121, 124, 127, 142, 358
FspBI CTAG 2 cut(s) 597, 1005
GlaI GCGC 2 cut(s) 268, 291
GluI GCNGC 8 cut(s) 42, 96, 99, 121, 124, 127, 142, 358
GsaI CCCAGC 1 cut(s) 962
HaeII RGCGCY 1 cut(s) 293
HaeIII GGCC 4 cut(s) 14, 101, 176, 351
HapII CCGG 2 cut(s) 8, 293
HhaI GCGC 2 cut(s) 269, 292
Hin1II CATG 1 cut(s) 398
Hin6I GCGC 2 cut(s) 267, 290
HinP1I GCGC 2 cut(s) 267, 290
HincII GTYRAC 2 cut(s) 643, 1036
HindII GTYRAC 2 cut(s) 643, 1036
HinfI GANTC 8 cut(s) 4, 107, 317, 370, 553, 593, 700, 838
HpaII CCGG 2 cut(s) 8, 293
HphI GGTGA 6 cut(s) 39, 379, 443, 602, 770, 895
Hpy166II GTNNAC 4 cut(s) 285, 328, 643, 1036
Hpy188I TCNGA 4 cut(s) 115, 364, 570, 790
Hpy188III TCNNGA 1 cut(s) 988
Hpy8I GTNNAC 4 cut(s) 285, 328, 643, 1036
Hpy99I CGWCG 2 cut(s) 95, 325
HpyAV CCTTC 4 cut(s) 53, 201, 255, 1050
HpyCH4III ACNGT 6 cut(s) 402, 529, 640, 724, 937, 966
HpyCH4IV ACGT 2 cut(s) 306, 1017
HpyCH4V TGCA 5 cut(s) 65, 256, 932, 974, 1063
HpyF10VI GCNNNNNNNGC 9 cut(s) 95, 126, 132, 141, 357, 387, 494, 913, 938
HpyF3I CTNAG 4 cut(s) 105, 363, 908, 1049
HpySE526I ACGT 2 cut(s) 306, 1017
Hsp92II CATG 1 cut(s) 398
HspAI GCGC 2 cut(s) 267, 290
Kzo9I GATC 6 cut(s) 436, 533, 570, 627, 766, 947
LguI GCTCTTC 1 cut(s) 1002
LmnI GCTCC 3 cut(s) 386, 461, 913
Lsp1109I GCAGC 2 cut(s) 28, 132
LweI GCATC 4 cut(s) 484, 704, 894, 1050
MaeI CTAG 2 cut(s) 597, 1005
MaeII ACGT 2 cut(s) 306, 1017
MaeIII GTNAC 6 cut(s) 188, 307, 590, 679, 758, 799
MalI GATC 6 cut(s) 438, 535, 572, 629, 768, 949
MboI GATC 6 cut(s) 436, 533, 570, 627, 766, 947
MboII GAAGA 6 cut(s) 47, 178, 236, 446, 989, 989
MfeI CAATTG 1 cut(s) 583
MflI RGATCY 1 cut(s) 766
MhlI GDGCHC 1 cut(s) 466
MlsI TGGCCA 2 cut(s) 14, 351
MluCI AATT 2 cut(s) 258, 583
MluNI TGGCCA 2 cut(s) 14, 351
MlyI GAGTC 4 cut(s) 116, 326, 379, 587
MmeI TCCRAC 1 cut(s) 691
Mox20I TGGCCA 2 cut(s) 14, 351
MscI TGGCCA 2 cut(s) 14, 351
Msp20I TGGCCA 2 cut(s) 14, 351
MspA1I CMGCKG 2 cut(s) 123, 542
MspI CCGG 2 cut(s) 8, 293
MunI CAATTG 1 cut(s) 583
Mva1269I GAATGC 2 cut(s) 836, 997
MvnI CGCG 1 cut(s) 95
MwoI GCNNNNNNNGC 9 cut(s) 95, 126, 132, 141, 357, 387, 494, 913, 938
NdeII GATC 6 cut(s) 436, 533, 570, 627, 766, 947
NlaIII CATG 1 cut(s) 398
NlaIV GGNNCC 1 cut(s) 463
NmuCI GTSAC 3 cut(s) 307, 590, 758
NspV TTCGAA 1 cut(s) 1000
PciSI GCTCTTC 1 cut(s) 1002
PcsI WCGNNNNNNNCGW 1 cut(s) 317
PctI GAATGC 2 cut(s) 836, 997
PfeI GAWTC 4 cut(s) 4, 553, 700, 838
PkrI GCNGC 8 cut(s) 43, 97, 100, 122, 125, 128, 143, 359
PleI GAGTC 4 cut(s) 115, 325, 378, 587
PpsI GAGTC 4 cut(s) 115, 325, 378, 587
Psp1406I AACGTT 1 cut(s) 1017
PspEI GGTNACC 1 cut(s) 758
PspFI CCCAGC 1 cut(s) 958
PspN4I GGNNCC 1 cut(s) 463
PspPI GGNCC 1 cut(s) 174
PsuI RGATCY 1 cut(s) 766
PvuII CAGCTG 1 cut(s) 542
RsaI GTAC 3 cut(s) 286, 305, 1009
RsaNI GTAC 3 cut(s) 285, 304, 1008
SalI GTCGAC 1 cut(s) 641
SapI GCTCTTC 1 cut(s) 1002
SatI GCNGC 8 cut(s) 42, 96, 99, 121, 124, 127, 142, 358
Sau3AI GATC 6 cut(s) 436, 533, 570, 627, 766, 947
Sau96I GGNCC 1 cut(s) 174
ScaI AGTACT 1 cut(s) 1009
SchI GAGTC 4 cut(s) 116, 326, 379, 587
SduI GDGCHC 1 cut(s) 466
SfaNI GCATC 4 cut(s) 484, 704, 894, 1050
SfcI CTRYAG 1 cut(s) 720
SfuI TTCGAA 1 cut(s) 1000
SmlI CTYRAG 1 cut(s) 986
SmoI CTYRAG 1 cut(s) 986
SpeI ACTAGT 1 cut(s) 1004
Sse9I AATT 2 cut(s) 258, 583
SsiI CCGC 9 cut(s) 95, 98, 123, 126, 141, 279, 357, 429, 588
SspMI CTAG 2 cut(s) 597, 1005
TaaI ACNGT 6 cut(s) 402, 529, 640, 724, 937, 966
TaiI ACGT 2 cut(s) 309, 1020
TaqI TCGA 3 cut(s) 536, 642, 1000
TasI AATT 2 cut(s) 258, 583
TatI WGTACW 2 cut(s) 284, 1007
TauI GCSGC 6 cut(s) 98, 101, 126, 129, 144, 360
TfiI GAWTC 4 cut(s) 4, 553, 700, 838
TscAI CASTG 3 cut(s) 76, 109, 446
TseFI GTSAC 3 cut(s) 307, 590, 758
TseI GCWGC 2 cut(s) 41, 120
Tsp45I GTSAC 3 cut(s) 307, 590, 758
TspDTI ATGAA 6 cut(s) 17, 48, 411, 681, 843, 891
TspGWI ACGGA 1 cut(s) 326
TspRI CASTG 3 cut(s) 76, 109, 446
XapI RAATTY 1 cut(s) 258
XmiI GTMKAC 1 cut(s) 642
XspI CTAG 2 cut(s) 597, 1005
ZrmI AGTACT 1 cut(s) 1009
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.