Rh2DG433700

Sigma factor PP2C-like phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0018325
Physical Location & Seq
Forward (+)
62683644 .. 62688799
5156 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG433700.1

Sequence Viewer

Length: 633 bp
ATGGGAGCCCCTGTTAGGGCTTACAACAGAGCGGATGATGCTTTTATAGACAAGACATTAGCACCAGATACAGTTGGATCGACAGCTGTGGCAGTGATTCTGTCAGCCTGTCAGATCATTGTTGCCAATTACGGTGACTCTAGTGCTGTGCTTTGTCGTGGAAATCAAGTGATCCCTTTGACTGTCGACCACAAGCCTGACAGAGACGATGAAGTAATTAGAGTTACTGAAGCAGGGGGCAGGATTCTGTATGTTGGATGCCCTCCAGTTGAGGGGGTTCTCGCTATGACAAGAGCAATAGGTGACCAAGATCTAAAGCCGTGGATTATTTCAGAGCCAGAAGTTACTTTTTTTACCAGAATTGATACTGATGAATGCTTGATTCTGGCGAGTGACGGCTTATGGGATGTGATGTCAAATGAAAAGGTGATGGCGATGGCTCGCAGGATGCTGAGGGAGCTTCGCAATCAGTATGCAACGGTAGCCAATGGATCAGTCAGCCCAGCACAGTATGTTGCAGAAGAAGTCCTCAAGAATGCTCTTCGAACTAGTACTGATAACGTTTCCATTATTGTTGTTGACCTGAAAAGGCTGAGGAGAAGGATGCAACAAAAGGCAGAAGCCGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

210

Amino Acids

23.07

Weight (kDa)

5.02

Isoelectric Point (pI)

41.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 15 - 179 8.1e-45 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17545
rosa_chinensis RchiOBHm_Chr2g0140491
rosa_laevigata RLG00000019871
rosa_rugosa Rorug02G0363300
rosa_samantha Rh2AG413800 Rh2AG413900 Rh2BG424000 Rh2CG400200 Rh2CG400300 Rh2DG433700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 32
AccI GTMKAC 1 cut(s) 186
AciI CCGC 1 cut(s) 32
AclI AACGTT 1 cut(s) 561
AclWI GGATC 3 cut(s) 85, 166, 499
AcuI CTGAAG 1 cut(s) 249
AfaI GTAC 1 cut(s) 553
AfiI CCNNNNNNNGG 3 cut(s) 15, 16, 272
AhlI ACTAGT 1 cut(s) 548
AluBI AGCT 2 cut(s) 86, 460
AluI AGCT 2 cut(s) 86, 460
Alw26I GTCTC 1 cut(s) 198
AlwI GGATC 3 cut(s) 85, 166, 499
AsuHPI GGTGA 3 cut(s) 146, 314, 439
AsuII TTCGAA 1 cut(s) 544
BanII GRGCYC 1 cut(s) 10
BbvCI CCTCAGC 2 cut(s) 452, 593
BccI CCATC 2 cut(s) 424, 430
BceAI ACGGC 2 cut(s) 304, 412
BcoDI GTCTC 1 cut(s) 198
BcuI ACTAGT 1 cut(s) 548
BfaI CTAG 2 cut(s) 141, 549
BglII AGATCT 1 cut(s) 310
BmcAI AGTACT 1 cut(s) 553
BmiI GGNNCC 1 cut(s) 7
BmsI GCATC 4 cut(s) 28, 248, 438, 594
BpmI CTGGAG 1 cut(s) 249
Bpu10I CCTNAGC 2 cut(s) 452, 593
Bpu14I TTCGAA 1 cut(s) 544
BpuEI CTTGAG 1 cut(s) 515
BsaJI CCNNGG 1 cut(s) 320
BsaXI ACNNNNNCTCC 1 cut(s) 27
Bsc4I CCNNNNNNNGG 3 cut(s) 15, 16, 272
Bse1I ACTGG 1 cut(s) 266
BseDI CCNNGG 1 cut(s) 320
BseGI GGATG 5 cut(s) 40, 263, 412, 453, 609
BseLI CCNNNNNNNGG 3 cut(s) 15, 16, 272
BseMII CTCAG 2 cut(s) 443, 584
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 1 cut(s) 610
BseYI CCCAGC 1 cut(s) 502
BslI CCNNNNNNNGG 3 cut(s) 15, 16, 272
BsmAI GTCTC 1 cut(s) 198
BsmBI CGTCTC 1 cut(s) 198
BsmI GAATGC 2 cut(s) 380, 541
Bsp119I TTCGAA 1 cut(s) 544
Bsp1286I GDGCHC 1 cut(s) 10
Bsp143I GATC 5 cut(s) 77, 114, 171, 310, 491
BspACI CCGC 1 cut(s) 32
BspCNI CTCAG 2 cut(s) 444, 585
BspLI GGNNCC 1 cut(s) 7
BspPI GGATC 3 cut(s) 85, 166, 499
BspQI GCTCTTC 1 cut(s) 546
BspT104I TTCGAA 1 cut(s) 544
BsrBI CCGCTC 1 cut(s) 32
BsrI ACTGG 1 cut(s) 266
BssECI CCNNGG 1 cut(s) 320
BssMI GATC 5 cut(s) 77, 114, 171, 310, 491
Bst4CI ACNGT 5 cut(s) 73, 134, 184, 481, 510
Bst6I CTCTTC 1 cut(s) 546
BstBI TTCGAA 1 cut(s) 544
BstC8I GCNNGC 1 cut(s) 442
BstDEI CTNAG 2 cut(s) 452, 593
BstDSI CCRYGG 1 cut(s) 320
BstEII GGTNACC 1 cut(s) 302
BstF5I GGATG 5 cut(s) 40, 263, 412, 453, 609
BstKTI GATC 5 cut(s) 80, 117, 174, 313, 494
BstMAI GTCTC 1 cut(s) 198
BstMBI GATC 5 cut(s) 77, 114, 171, 310, 491
BstMWI GCNNNNNNNGC 3 cut(s) 38, 457, 482
BstPI GGTNACC 1 cut(s) 302
BstX2I RGATCY 1 cut(s) 310
BstYI RGATCY 1 cut(s) 310
BtgI CCRYGG 1 cut(s) 320
BtgZI GCGATG 1 cut(s) 449
BtsCI GGATG 5 cut(s) 40, 263, 412, 453, 609
BtsI GCAGTG 1 cut(s) 99
BtsIMutI CAGTG 1 cut(s) 99
Cac8I GCNNGC 1 cut(s) 442
Csp6I GTAC 1 cut(s) 552
CviQI GTAC 1 cut(s) 552
DdeI CTNAG 2 cut(s) 452, 593
DpnI GATC 5 cut(s) 79, 116, 173, 312, 493
DpnII GATC 5 cut(s) 77, 114, 171, 310, 491
Eam1104I CTCTTC 1 cut(s) 546
EarI CTCTTC 1 cut(s) 546
Eco24I GRGCYC 1 cut(s) 10
Eco57I CTGAAG 1 cut(s) 249
Eco91I GGTNACC 1 cut(s) 302
EcoO65I GGTNACC 1 cut(s) 302
EcoT38I GRGCYC 1 cut(s) 10
Esp3I CGTCTC 1 cut(s) 198
FaiI YATR 8 cut(s) 47, 252, 287, 403, 474, 513, 629, 631
FblI GTMKAC 1 cut(s) 186
FokI GGATG 5 cut(s) 47, 270, 419, 460, 616
FriOI GRGCYC 1 cut(s) 10
FspBI CTAG 2 cut(s) 141, 549
GsaI CCCAGC 1 cut(s) 506
GsuI CTGGAG 1 cut(s) 249
HincII GTYRAC 2 cut(s) 187, 580
HindII GTYRAC 2 cut(s) 187, 580
HinfI GANTC 4 cut(s) 97, 137, 244, 382
HphI GGTGA 3 cut(s) 146, 314, 439
Hpy166II GTNNAC 2 cut(s) 187, 580
Hpy188I TCNGA 2 cut(s) 114, 334
Hpy188III TCNNGA 1 cut(s) 532
Hpy8I GTNNAC 2 cut(s) 187, 580
HpyAV CCTTC 1 cut(s) 594
HpyCH4III ACNGT 5 cut(s) 73, 134, 184, 481, 510
HpyCH4IV ACGT 1 cut(s) 561
HpyCH4V TGCA 3 cut(s) 476, 518, 607
HpyF10VI GCNNNNNNNGC 3 cut(s) 38, 457, 482
HpyF3I CTNAG 2 cut(s) 452, 593
HpySE526I ACGT 1 cut(s) 561
Kzo9I GATC 5 cut(s) 77, 114, 171, 310, 491
LguI GCTCTTC 1 cut(s) 546
LmnI GCTCC 2 cut(s) 5, 457
LweI GCATC 4 cut(s) 28, 248, 438, 594
MaeI CTAG 2 cut(s) 141, 549
MaeII ACGT 1 cut(s) 561
MaeIII GTNAC 5 cut(s) 134, 223, 302, 343, 392
MalI GATC 5 cut(s) 79, 116, 173, 312, 493
MbiI CCGCTC 1 cut(s) 32
MboI GATC 5 cut(s) 77, 114, 171, 310, 491
MboII GAAGA 2 cut(s) 533, 533
MflI RGATCY 1 cut(s) 310
MhlI GDGCHC 1 cut(s) 10
MluCI AATT 3 cut(s) 127, 216, 360
MlyI GAGTC 1 cut(s) 131
MmeI TCCRAC 2 cut(s) 55, 235
MnlI CCTC 5 cut(s) 265, 273, 447, 539, 588
MspA1I CMGCKG 1 cut(s) 86
Mva1269I GAATGC 2 cut(s) 380, 541
MwoI GCNNNNNNNGC 3 cut(s) 38, 457, 482
NdeII GATC 5 cut(s) 77, 114, 171, 310, 491
NlaIV GGNNCC 1 cut(s) 7
NmuCI GTSAC 3 cut(s) 134, 302, 392
NspV TTCGAA 1 cut(s) 544
PciSI GCTCTTC 1 cut(s) 546
PctI GAATGC 2 cut(s) 380, 541
PfeI GAWTC 3 cut(s) 97, 244, 382
PleI GAGTC 1 cut(s) 131
PpsI GAGTC 1 cut(s) 131
Psp1406I AACGTT 1 cut(s) 561
PspEI GGTNACC 1 cut(s) 302
PspFI CCCAGC 1 cut(s) 502
PspN4I GGNNCC 1 cut(s) 7
PsuI RGATCY 1 cut(s) 310
PvuII CAGCTG 1 cut(s) 86
RsaI GTAC 1 cut(s) 553
RsaNI GTAC 1 cut(s) 552
SalI GTCGAC 1 cut(s) 185
SapI GCTCTTC 1 cut(s) 546
Sau3AI GATC 5 cut(s) 77, 114, 171, 310, 491
ScaI AGTACT 1 cut(s) 553
SchI GAGTC 1 cut(s) 131
SduI GDGCHC 1 cut(s) 10
SetI ASST 6 cut(s) 88, 304, 429, 462, 564, 585
SfaNI GCATC 4 cut(s) 28, 248, 438, 594
SfuI TTCGAA 1 cut(s) 544
SmlI CTYRAG 1 cut(s) 530
SmoI CTYRAG 1 cut(s) 530
SpeI ACTAGT 1 cut(s) 548
Sse9I AATT 3 cut(s) 127, 216, 360
SsiI CCGC 1 cut(s) 32
SspMI CTAG 2 cut(s) 141, 549
TaaI ACNGT 5 cut(s) 73, 134, 184, 481, 510
TaiI ACGT 1 cut(s) 564
TaqI TCGA 3 cut(s) 80, 186, 544
TasI AATT 3 cut(s) 127, 216, 360
TatI WGTACW 1 cut(s) 551
TfiI GAWTC 3 cut(s) 97, 244, 382
TscAI CASTG 1 cut(s) 99
TseFI GTSAC 3 cut(s) 134, 302, 392
Tsp45I GTSAC 3 cut(s) 134, 302, 392
TspDTI ATGAA 3 cut(s) 225, 387, 435
TspRI CASTG 1 cut(s) 99
XmiI GTMKAC 1 cut(s) 186
XspI CTAG 2 cut(s) 141, 549
ZrmI AGTACT 1 cut(s) 553
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.