RLG00000019871

Sigma factor PP2C-like phosphatases

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0018325
Physical Location & Seq
Forward (+)
57795759 .. 57797913
2155 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019871

Sequence Viewer

Length: 753 bp
ATGGAATTGGACAATGCAAAACTAACATACTGGATTGTTGTTTGCAAAGGCCTTGTCACATTTGTGAGTTGTTATTGCTCCAAAAGTCTCCATGAACTGTTGGCAGAGGAGTGGGAAAAAGGAAGCGGTGAAGATCAGTGGAATAAAAGGTGGATGGGAGCCCTTGTTAGGGCTTACAACAGAGCAGATGATGCTTTTATAGACAAGACATTAGCACCAGATACAGTTGGATCAACAGCTGTGGTAGTGATTCTGTCAGCTTGTCAGATCATTGTTGCCAATTGCGGTGACTCTAGGGCTGTGCTTTGTCATGGAAATCAAATGATCCCTTTGACTGTCGACCACAAGCCTGACAGATACGATGAAGTAATTAGAGTTACTGAAGCAGGGGGCAGGATTCTGTATGTTGGATGCCCTACAGTTGAGGGGGTACTCACTATGACAAGAGCAATAGAGCCAGAAGTTACTTTTTTGACTAGAACTGATACTGACGAATGCTTGATTCTGGCGAGTGATGGCTTATGGGATGTGATGTCTAATGAAAAGGTGATGGCGATGGCTCGCAGGATGCTGAGGGAGCTTCGCAAGCAGTATGAAATGGCAGCCAATGGATCAGTCAGCCCAGCACGGTATGTTGCAGAAGAAGTCCTCAAGAATGCTCTTCGAACTAGTACTGATAACGTTTCCATTATTGTTGTTGACCTGAAAAGGCTGAGGAGAAGAATGCAACAAAAGGCAGAAGCTGATATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04016 ko04075 ko04931 map04016 map04075 map04931
Pfam Domains
Protein Families
PP2C

Protein Analysis

251

Amino Acids

28.02

Weight (kDa)

5.55

Isoelectric Point (pI)

43.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 20 - 209 5.7e-38 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018325)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G17545
rosa_chinensis RchiOBHm_Chr2g0140491
rosa_laevigata RLG00000019871
rosa_rugosa Rorug02G0363300
rosa_samantha Rh2AG413800 Rh2AG413900 Rh2BG424000 Rh2CG400200 Rh2CG400300 Rh2DG433700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 339
AciI CCGC 2 cut(s) 126, 285
AclI AACGTT 1 cut(s) 681
AclWI GGATC 3 cut(s) 238, 319, 619
AcuI CTGAAG 1 cut(s) 402
AfaI GTAC 2 cut(s) 432, 673
AfiI CCNNNNNNNGG 2 cut(s) 168, 169
AhlI ACTAGT 1 cut(s) 668
AleI CACNNNNGTG 1 cut(s) 62
AluBI AGCT 4 cut(s) 239, 260, 580, 743
AluI AGCT 4 cut(s) 239, 260, 580, 743
Alw26I GTCTC 1 cut(s) 92
AlwI GGATC 3 cut(s) 238, 319, 619
AlwNI CAGNNNCTG 1 cut(s) 743
AoxI GGCC 1 cut(s) 49
ApeKI GCWGC 1 cut(s) 602
ArsI GACNNNNNNTTYG 2 cut(s) 39, 71
AsuHPI GGTGA 3 cut(s) 140, 299, 559
AsuII TTCGAA 1 cut(s) 664
BanII GRGCYC 1 cut(s) 163
BbvCI CCTCAGC 2 cut(s) 572, 713
BbvI GCAGC 1 cut(s) 614
BccI CCATC 4 cut(s) 148, 509, 544, 550
BcoDI GTCTC 1 cut(s) 92
BcuI ACTAGT 1 cut(s) 668
BfaI CTAG 3 cut(s) 294, 477, 669
BfmI CTRYAG 1 cut(s) 417
BisI GCNGC 1 cut(s) 603
BlsI GCNGC 1 cut(s) 604
BmcAI AGTACT 1 cut(s) 673
BmiI GGNNCC 1 cut(s) 160
BmsI GCATC 3 cut(s) 181, 401, 558
Bpu10I CCTNAGC 2 cut(s) 572, 713
Bpu14I TTCGAA 1 cut(s) 664
BpuEI CTTGAG 1 cut(s) 635
BsaXI ACNNNNNCTCC 2 cut(s) 150, 180
Bsc4I CCNNNNNNNGG 2 cut(s) 168, 169
Bse1I ACTGG 1 cut(s) 35
BseGI GGATG 4 cut(s) 159, 416, 532, 573
BseLI CCNNNNNNNGG 2 cut(s) 168, 169
BseMII CTCAG 2 cut(s) 563, 704
BseNI ACTGG 1 cut(s) 35
BseRI GAGGAG 2 cut(s) 122, 730
BseXI GCAGC 1 cut(s) 614
BseYI CCCAGC 1 cut(s) 622
BshFI GGCC 1 cut(s) 51
BslI CCNNNNNNNGG 2 cut(s) 168, 169
BsmAI GTCTC 1 cut(s) 92
BsmI GAATGC 3 cut(s) 500, 661, 729
BsnI GGCC 1 cut(s) 51
Bsp119I TTCGAA 1 cut(s) 664
Bsp1286I GDGCHC 1 cut(s) 163
Bsp143I GATC 5 cut(s) 133, 230, 267, 324, 611
BspACI CCGC 2 cut(s) 126, 285
BspANI GGCC 1 cut(s) 51
BspCNI CTCAG 2 cut(s) 564, 705
BspLI GGNNCC 1 cut(s) 160
BspPI GGATC 3 cut(s) 238, 319, 619
BspQI GCTCTTC 1 cut(s) 666
BspT104I TTCGAA 1 cut(s) 664
BsrI ACTGG 1 cut(s) 35
BssMI GATC 5 cut(s) 133, 230, 267, 324, 611
Bst4CI ACNGT 5 cut(s) 99, 226, 337, 421, 630
Bst6I CTCTTC 1 cut(s) 666
BstAPI GCANNNNNTGC 1 cut(s) 191
BstBI TTCGAA 1 cut(s) 664
BstC8I GCNNGC 2 cut(s) 562, 587
BstDEI CTNAG 2 cut(s) 572, 713
BstF5I GGATG 4 cut(s) 159, 416, 532, 573
BstKTI GATC 5 cut(s) 136, 233, 270, 327, 614
BstMAI GTCTC 1 cut(s) 92
BstMBI GATC 5 cut(s) 133, 230, 267, 324, 611
BstMWI GCNNNNNNNGC 3 cut(s) 191, 577, 586
BstSFI CTRYAG 1 cut(s) 417
BstV1I GCAGC 1 cut(s) 614
BsuRI GGCC 1 cut(s) 51
BtgZI GCGATG 1 cut(s) 569
BtsCI GGATG 4 cut(s) 159, 416, 532, 573
BtsIMutI CAGTG 1 cut(s) 143
Cac8I GCNNGC 2 cut(s) 562, 587
CaiI CAGNNNCTG 1 cut(s) 743
Csp6I GTAC 2 cut(s) 431, 672
CspCI CAANNNNNGTGG 2 cut(s) 222, 257
CviAII CATG 2 cut(s) 92, 311
CviQI GTAC 2 cut(s) 431, 672
DdeI CTNAG 2 cut(s) 572, 713
DpnI GATC 5 cut(s) 135, 232, 269, 326, 613
DpnII GATC 5 cut(s) 133, 230, 267, 324, 611
Eam1104I CTCTTC 1 cut(s) 666
EarI CTCTTC 1 cut(s) 666
Eco147I AGGCCT 1 cut(s) 51
Eco24I GRGCYC 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 402
EcoT38I GRGCYC 1 cut(s) 163
FaeI CATG 2 cut(s) 95, 314
FatI CATG 2 cut(s) 91, 310
FblI GTMKAC 1 cut(s) 339
Fnu4HI GCNGC 1 cut(s) 603
FokI GGATG 4 cut(s) 166, 423, 539, 580
FriOI GRGCYC 1 cut(s) 163
Fsp4HI GCNGC 1 cut(s) 603
FspBI CTAG 3 cut(s) 294, 477, 669
GluI GCNGC 1 cut(s) 603
GsaI CCCAGC 1 cut(s) 626
HaeIII GGCC 1 cut(s) 51
Hin1II CATG 2 cut(s) 95, 314
HincII GTYRAC 2 cut(s) 340, 700
HindII GTYRAC 2 cut(s) 340, 700
HinfI GANTC 4 cut(s) 250, 290, 397, 502
HphI GGTGA 3 cut(s) 140, 299, 559
Hpy166II GTNNAC 2 cut(s) 340, 700
Hpy188I TCNGA 1 cut(s) 267
Hpy188III TCNNGA 1 cut(s) 652
Hpy8I GTNNAC 2 cut(s) 340, 700
HpyCH4III ACNGT 5 cut(s) 99, 226, 337, 421, 630
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 4 cut(s) 17, 45, 638, 727
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 577, 586
HpyF3I CTNAG 2 cut(s) 572, 713
HpySE526I ACGT 1 cut(s) 681
Hsp92II CATG 2 cut(s) 95, 314
Kzo9I GATC 5 cut(s) 133, 230, 267, 324, 611
LguI GCTCTTC 1 cut(s) 666
LmnI GCTCC 3 cut(s) 83, 158, 577
Lsp1109I GCAGC 1 cut(s) 614
LweI GCATC 3 cut(s) 181, 401, 558
MaeI CTAG 3 cut(s) 294, 477, 669
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 4 cut(s) 55, 287, 376, 463
MalI GATC 5 cut(s) 135, 232, 269, 326, 613
MboI GATC 5 cut(s) 133, 230, 267, 324, 611
MboII GAAGA 4 cut(s) 143, 653, 653, 732
MfeI CAATTG 1 cut(s) 280
MhlI GDGCHC 1 cut(s) 163
MluCI AATT 3 cut(s) 5, 280, 369
MlyI GAGTC 1 cut(s) 284
MmeI TCCRAC 2 cut(s) 208, 388
MnlI CCTC 5 cut(s) 100, 418, 567, 659, 708
MslI CAYNNNNRTG 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 239
MunI CAATTG 1 cut(s) 280
Mva1269I GAATGC 3 cut(s) 500, 661, 729
MwoI GCNNNNNNNGC 3 cut(s) 191, 577, 586
NdeII GATC 5 cut(s) 133, 230, 267, 324, 611
NlaIII CATG 2 cut(s) 95, 314
NlaIV GGNNCC 1 cut(s) 160
NmuCI GTSAC 2 cut(s) 55, 287
NspV TTCGAA 1 cut(s) 664
OliI CACNNNNGTG 1 cut(s) 62
PceI AGGCCT 1 cut(s) 51
PciSI GCTCTTC 1 cut(s) 666
PctI GAATGC 3 cut(s) 500, 661, 729
PfeI GAWTC 3 cut(s) 250, 397, 502
PkrI GCNGC 1 cut(s) 604
PleI GAGTC 1 cut(s) 284
PpsI GAGTC 1 cut(s) 284
Psp1406I AACGTT 1 cut(s) 681
PspFI CCCAGC 1 cut(s) 622
PspN4I GGNNCC 1 cut(s) 160
PstNI CAGNNNCTG 1 cut(s) 743
PvuII CAGCTG 1 cut(s) 239
RsaI GTAC 2 cut(s) 432, 673
RsaNI GTAC 2 cut(s) 431, 672
RseI CAYNNNNRTG 1 cut(s) 62
SalI GTCGAC 1 cut(s) 338
SapI GCTCTTC 1 cut(s) 666
SatI GCNGC 1 cut(s) 603
Sau3AI GATC 5 cut(s) 133, 230, 267, 324, 611
ScaI AGTACT 1 cut(s) 673
SchI GAGTC 1 cut(s) 284
SduI GDGCHC 1 cut(s) 163
SetI ASST 8 cut(s) 152, 241, 262, 549, 582, 684, 705, 745
SfaNI GCATC 3 cut(s) 181, 401, 558
SfcI CTRYAG 1 cut(s) 417
SfuI TTCGAA 1 cut(s) 664
SmiMI CAYNNNNRTG 1 cut(s) 62
SmlI CTYRAG 1 cut(s) 650
SmoI CTYRAG 1 cut(s) 650
SpeI ACTAGT 1 cut(s) 668
Sse9I AATT 3 cut(s) 5, 280, 369
SseBI AGGCCT 1 cut(s) 51
SsiI CCGC 2 cut(s) 126, 285
SspMI CTAG 3 cut(s) 294, 477, 669
StuI AGGCCT 1 cut(s) 51
TaaI ACNGT 5 cut(s) 99, 226, 337, 421, 630
TaiI ACGT 1 cut(s) 684
TaqI TCGA 2 cut(s) 339, 664
TasI AATT 3 cut(s) 5, 280, 369
TatI WGTACW 1 cut(s) 671
TfiI GAWTC 3 cut(s) 250, 397, 502
TscAI CASTG 1 cut(s) 143
TseFI GTSAC 2 cut(s) 55, 287
TseI GCWGC 1 cut(s) 602
Tsp45I GTSAC 2 cut(s) 55, 287
TspDTI ATGAA 4 cut(s) 108, 378, 555, 609
TspRI CASTG 1 cut(s) 143
XmiI GTMKAC 1 cut(s) 339
XspI CTAG 3 cut(s) 294, 477, 669
ZrmI AGTACT 1 cut(s) 673
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.