RchiOBHm_Chr1g0359511

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0018517
Physical Location & Seq
Forward (+)
51477559 .. 51480275
2717 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 645 bp
ATGGGTTGCGCAAGCTCAAACAAAGCCAGAGTCAAAGCTGGCCCAAGTGCCAATTTAGAGCTTAATCCGGCTATTCTTGCTTTTTCTGTGAGTGAGGTAGAGTCTTTGTATGAGCTATATAAGAAAATAAGCAGCTCTGTAGTTCAAGATGGGCTTATACACAAGAAAGAGTTGCAGCTTGCGCTTTTTCAAAACAGCAGTAAGCAGAACCTCTTTTTCGACAGGATATTTGATCTTTTTAATGTCAATAACAATGGGCGTATTGAGTTTGAAGAGTTTGTTCATACATTGAGTGTTTTCCACCCAAGAACTCCTGATGAAGTCAAAATTACATATGCTTTTAAGTTATATGATTTGAGGCACACTGGTTACATCGAGCGTGAGGAGTTGAAGGAGATGGTGGTGGCTCTTCTGAACGAATCTGATCTGATTCTTGCAGATGATATTGTTGAAATGATTGTGAATAAGACATTCATGGAAGCAGATGGAAAAGCAGACGGTAGGATTGATGAAGAAGAGTGGAAGGAATACGTAGCAAAAAACCCTTCTCTACTGAAAAACATGACTCTTCCATACTTGATGGACATATCTCTAGCATTTCCCAGCTTTGTGCTGAATACTCAAGCAGAAGAATCTCAATTGTAG

Protein Analysis

214

Amino Acids

24.42

Weight (kDa)

4.83

Isoelectric Point (pI)

39.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 108 - 177 7.7e-09 EF-hand domain pair
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018517)

Species Orthologous Gene IDs
prunus_persica Prupe.2G188700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0359511 RchiOBHm_Chr1g0359521
rosa_multiflora Rmu_sc0002556.1_g000015
rosa_roxburghii Rroxscaffold_4G00296920
rosa_rugosa Rorug01G0272000
rosa_samantha Rh1BG251400 Rh1DG280500
rosa_wichuraiana Rw1G025360 Rw1G025370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 10
AgsI TTSAA 5 cut(s) 146, 191, 272, 391, 452
AluBI AGCT 7 cut(s) 15, 38, 61, 115, 135, 178, 606
AluI AGCT 7 cut(s) 15, 38, 61, 115, 135, 178, 606
AoxI GGCC 1 cut(s) 40
ApeKI GCWGC 2 cut(s) 132, 175
ArsI GACNNNNNNTTYG 4 cut(s) 15, 47, 212, 244
AspLEI GCGC 2 cut(s) 11, 184
AspS9I GGNCC 1 cut(s) 41
BbvI GCAGC 2 cut(s) 144, 187
BccI CCATC 4 cut(s) 143, 391, 479, 574
BfaI CTAG 1 cut(s) 593
BfmI CTRYAG 1 cut(s) 138
BisI GCNGC 2 cut(s) 133, 176
BlsI GCNGC 2 cut(s) 134, 177
BmgT120I GGNCC 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 606
BsaAI YACGTR 1 cut(s) 532
Bse1I ACTGG 1 cut(s) 370
BseNI ACTGG 1 cut(s) 370
BseRI GAGGAG 1 cut(s) 398
BseXI GCAGC 2 cut(s) 144, 187
BseYI CCCAGC 1 cut(s) 602
BshFI GGCC 1 cut(s) 42
BsiSI CCGG 1 cut(s) 68
BsnI GGCC 1 cut(s) 42
Bsp143I GATC 2 cut(s) 232, 424
BspANI GGCC 1 cut(s) 42
BspQI GCTCTTC 1 cut(s) 414
BsrI ACTGG 1 cut(s) 370
BssMI GATC 2 cut(s) 232, 424
Bst4CI ACNGT 1 cut(s) 500
Bst6I CTCTTC 4 cut(s) 267, 414, 510, 573
BstBAI YACGTR 1 cut(s) 532
BstC8I GCNNGC 3 cut(s) 13, 40, 180
BstHHI GCGC 2 cut(s) 11, 184
BstKTI GATC 2 cut(s) 235, 427
BstMBI GATC 2 cut(s) 232, 424
BstMWI GCNNNNNNNGC 2 cut(s) 77, 181
BstSFI CTRYAG 1 cut(s) 138
BstSNI TACGTA 1 cut(s) 532
BstV1I GCAGC 2 cut(s) 144, 187
BsuRI GGCC 1 cut(s) 42
BtsIMutI CAGTG 1 cut(s) 363
Cac8I GCNNGC 3 cut(s) 13, 40, 180
CfoI GCGC 2 cut(s) 11, 184
Cfr13I GGNCC 1 cut(s) 41
CviAII CATG 2 cut(s) 475, 562
DpnI GATC 2 cut(s) 234, 426
DpnII GATC 2 cut(s) 232, 424
Eam1104I CTCTTC 4 cut(s) 267, 414, 510, 573
EarI CTCTTC 4 cut(s) 267, 414, 510, 573
Eco105I TACGTA 1 cut(s) 532
FaeI CATG 2 cut(s) 478, 565
FalI AAGNNNNNCTT 2 cut(s) 138, 170
FatI CATG 2 cut(s) 474, 561
FauNDI CATATG 1 cut(s) 334
Fnu4HI GCNGC 2 cut(s) 133, 176
Fsp4HI GCNGC 2 cut(s) 133, 176
FspBI CTAG 1 cut(s) 593
FspI TGCGCA 1 cut(s) 10
GlaI GCGC 2 cut(s) 10, 183
GluI GCNGC 2 cut(s) 133, 176
GsaI CCCAGC 1 cut(s) 606
HaeIII GGCC 1 cut(s) 42
HapII CCGG 1 cut(s) 68
HhaI GCGC 2 cut(s) 11, 184
Hin1II CATG 2 cut(s) 478, 565
Hin6I GCGC 2 cut(s) 9, 182
HinP1I GCGC 2 cut(s) 9, 182
HinfI GANTC 6 cut(s) 30, 101, 419, 430, 565, 632
HpaII CCGG 1 cut(s) 68
Hpy188I TCNGA 3 cut(s) 414, 424, 429
Hpy188III TCNNGA 2 cut(s) 146, 314
HpyAV CCTTC 3 cut(s) 385, 517, 555
HpyCH4III ACNGT 1 cut(s) 500
HpyCH4IV ACGT 1 cut(s) 531
HpyCH4V TGCA 2 cut(s) 175, 437
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 181
HpySE526I ACGT 1 cut(s) 531
Hsp92II CATG 2 cut(s) 478, 565
HspAI GCGC 2 cut(s) 9, 182
Kzo9I GATC 2 cut(s) 232, 424
LguI GCTCTTC 1 cut(s) 414
LpnPI CCDG 7 cut(s) 24, 40, 81, 208, 327, 351, 616
Lsp1109I GCAGC 2 cut(s) 144, 187
MaeI CTAG 1 cut(s) 593
MaeII ACGT 1 cut(s) 531
MaeIII GTNAC 1 cut(s) 368
MalI GATC 2 cut(s) 234, 426
MboI GATC 2 cut(s) 232, 424
MboII GAAGA 6 cut(s) 284, 401, 524, 527, 560, 641
MfeI CAATTG 1 cut(s) 638
MluCI AATT 3 cut(s) 52, 327, 638
MlyI GAGTC 3 cut(s) 39, 110, 559
MnlI CCTC 4 cut(s) 88, 221, 351, 376
MseI TTAA 3 cut(s) 63, 240, 342
MspI CCGG 1 cut(s) 68
MunI CAATTG 1 cut(s) 638
MwoI GCNNNNNNNGC 2 cut(s) 77, 181
NdeI CATATG 1 cut(s) 334
NdeII GATC 2 cut(s) 232, 424
NlaIII CATG 2 cut(s) 478, 565
NsbI TGCGCA 1 cut(s) 10
PciSI GCTCTTC 1 cut(s) 414
PfeI GAWTC 3 cut(s) 419, 430, 632
PkrI GCNGC 2 cut(s) 134, 177
PleI GAGTC 3 cut(s) 38, 109, 559
PpsI GAGTC 3 cut(s) 38, 109, 559
Ppu21I YACGTR 1 cut(s) 532
PspFI CCCAGC 1 cut(s) 602
PspPI GGNCC 1 cut(s) 41
SapI GCTCTTC 1 cut(s) 414
SaqAI TTAA 3 cut(s) 63, 240, 342
SatI GCNGC 2 cut(s) 133, 176
Sau3AI GATC 2 cut(s) 232, 424
Sau96I GGNCC 1 cut(s) 41
SchI GAGTC 3 cut(s) 39, 110, 559
SfcI CTRYAG 1 cut(s) 138
SmlI CTYRAG 1 cut(s) 621
SmoI CTYRAG 1 cut(s) 621
SnaBI TACGTA 1 cut(s) 532
Sse9I AATT 3 cut(s) 52, 327, 638
SspMI CTAG 1 cut(s) 593
TaaI ACNGT 1 cut(s) 500
TaiI ACGT 1 cut(s) 534
TaqI TCGA 2 cut(s) 219, 375
TasI AATT 3 cut(s) 52, 327, 638
TfiI GAWTC 3 cut(s) 419, 430, 632
Tru1I TTAA 3 cut(s) 63, 240, 342
Tru9I TTAA 3 cut(s) 63, 240, 342
TscAI CASTG 1 cut(s) 370
TseI GCWGC 2 cut(s) 132, 175
TspDTI ATGAA 4 cut(s) 272, 333, 463, 525
TspRI CASTG 1 cut(s) 370
XspI CTAG 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.