Rorug01G0272000

Calcineurin B-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0018517
Physical Location & Seq
Reverse (-)
38427179 .. 38427890
712 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0272000.1

Sequence Viewer

Length: 513 bp
ATGAACTTTGTAGGCTTCTTCAATGCTTTGTATAGGGATACCAACACATCAAGTGTAAACCTTGGACTAGGACAGATTGATTGGAAAAAGTTGTTGACTGAAACTTACGAGTTAACAGAAGAATGCTTGCAAATCAATTATTATGGTGCTAAAAGAACAGCTGAAACACTAATTCCACTCCTCCAGCTATCTGATTCACCAAGAATTGTTAATGTCTCATCCTCCAGGGGGAAGTTAAAGAGCATACCAAGTGATCGAGTGAAAGGAATTCTTAGTACTGACGTTGAGAACCTAAGCGAAGAGAGTGTAGATGAAATATTGACAGAGTTTCTAAACGACCTCAAGGAGAGTTTACTTGAAAGCAAGGGTTGGCCTTCCATGATGTCAGGCTATACAGTCTCAAAAGCAGCAATGAATGCATATACAAGGATTCTAGCCAAGAAGTACCCCGGTTTTCGCGTCAACTCTGTCTGCCCTGTGTTGGAGAGGAAAGATCCCACATTGGAAAAGTGA

Protein Analysis

170

Amino Acids

19.13

Weight (kDa)

5.65

Isoelectric Point (pI)

38.22

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0018517)

Species Orthologous Gene IDs
prunus_persica Prupe.2G188700_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0359511 RchiOBHm_Chr1g0359521
rosa_multiflora Rmu_sc0002556.1_g000015
rosa_roxburghii Rroxscaffold_4G00296920
rosa_rugosa Rorug01G0272000
rosa_samantha Rh1BG251400 Rh1DG280500
rosa_wichuraiana Rw1G025360 Rw1G025370

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 459
AclWI GGATC 1 cut(s) 488
AcsI RAATTY 1 cut(s) 267
AfaI GTAC 2 cut(s) 277, 446
AfiI CCNNNNNNNGG 2 cut(s) 228, 481
AgsI TTSAA 2 cut(s) 22, 359
AjnI CCWGG 1 cut(s) 224
AluBI AGCT 2 cut(s) 161, 187
AluI AGCT 2 cut(s) 161, 187
Alw26I GTCTC 2 cut(s) 220, 403
AlwI GGATC 1 cut(s) 488
AoxI GGCC 1 cut(s) 371
ApeKI GCWGC 1 cut(s) 407
ApoI RAATTY 1 cut(s) 267
AsuC2I CCSGG 1 cut(s) 450
AsuHPI GGTGA 1 cut(s) 189
BbvI GCAGC 1 cut(s) 419
BciT130I CCWGG 1 cut(s) 226
BciVI GTATCC 1 cut(s) 31
BcnI CCSGG 1 cut(s) 450
BcoDI GTCTC 2 cut(s) 220, 403
BfaI CTAG 2 cut(s) 68, 434
BfuI GTATCC 1 cut(s) 31
BisI GCNGC 1 cut(s) 408
BlsI GCNGC 1 cut(s) 409
BmcAI AGTACT 1 cut(s) 277
Bme1390I CCNGG 2 cut(s) 226, 450
BmrFI CCNGG 2 cut(s) 226, 450
BpmI CTGGAG 2 cut(s) 167, 208
Bpu10I CCTNAGC 1 cut(s) 293
BpuEI CTTGAG 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 450
BsaJI CCNNGG 3 cut(s) 61, 225, 448
Bsc4I CCNNNNNNNGG 2 cut(s) 228, 481
Bse3DI GCAATG 1 cut(s) 417
BseBI CCWGG 1 cut(s) 226
BseDI CCNNGG 3 cut(s) 61, 225, 448
BseGI GGATG 1 cut(s) 218
BseLI CCNNNNNNNGG 2 cut(s) 228, 481
BseMI GCAATG 1 cut(s) 417
BseRI GAGGAG 1 cut(s) 170
BseXI GCAGC 1 cut(s) 419
Bsh1236I CGCG 1 cut(s) 459
BshFI GGCC 1 cut(s) 373
BsiSI CCGG 1 cut(s) 450
BslI CCNNNNNNNGG 2 cut(s) 228, 481
BsmAI GTCTC 2 cut(s) 220, 403
BsmI GAATGC 2 cut(s) 128, 421
BsnI GGCC 1 cut(s) 373
Bsp143I GATC 2 cut(s) 253, 493
BspANI GGCC 1 cut(s) 373
BspFNI CGCG 1 cut(s) 459
BspPI GGATC 1 cut(s) 488
BsrDI GCAATG 1 cut(s) 417
BssECI CCNNGG 3 cut(s) 61, 225, 448
BssMI GATC 2 cut(s) 253, 493
BssT1I CCWWGG 1 cut(s) 61
Bst2UI CCWGG 1 cut(s) 226
Bst4CI ACNGT 1 cut(s) 397
Bst6I CTCTTC 1 cut(s) 294
BstAPI GCANNNNNTGC 1 cut(s) 416
BstC8I GCNNGC 1 cut(s) 128
BstDEI CTNAG 2 cut(s) 272, 293
BstF5I GGATG 1 cut(s) 218
BstFNI CGCG 1 cut(s) 459
BstKTI GATC 2 cut(s) 256, 496
BstMAI GTCTC 2 cut(s) 220, 403
BstMBI GATC 2 cut(s) 253, 493
BstMWI GCNNNNNNNGC 1 cut(s) 416
BstNI CCWGG 1 cut(s) 226
BstSCI CCNGG 2 cut(s) 224, 448
BstUI CGCG 1 cut(s) 459
BstV1I GCAGC 1 cut(s) 419
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BsuI GTATCC 1 cut(s) 31
BsuRI GGCC 1 cut(s) 373
BtsCI GGATG 1 cut(s) 218
Cac8I GCNNGC 1 cut(s) 128
CseI GACGC 1 cut(s) 448
Csp6I GTAC 2 cut(s) 276, 445
CviAII CATG 1 cut(s) 379
CviJI RGCY 6 cut(s) 15, 161, 187, 373, 390, 437
CviKI_1 RGCY 6 cut(s) 15, 161, 187, 373, 390, 437
CviQI GTAC 2 cut(s) 276, 445
DdeI CTNAG 2 cut(s) 272, 293
DpnI GATC 2 cut(s) 255, 495
DpnII GATC 2 cut(s) 253, 493
Eam1104I CTCTTC 1 cut(s) 294
EarI CTCTTC 1 cut(s) 294
Eco130I CCWWGG 1 cut(s) 61
EcoRI GAATTC 1 cut(s) 267
EcoRII CCWGG 1 cut(s) 224
EcoT14I CCWWGG 1 cut(s) 61
EcoT22I ATGCAT 1 cut(s) 421
ErhI CCWWGG 1 cut(s) 61
FaeI CATG 1 cut(s) 382
FaiI YATR 7 cut(s) 33, 144, 245, 380, 393, 421, 423
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FatI CATG 1 cut(s) 378
Fnu4HI GCNGC 1 cut(s) 408
FokI GGATG 1 cut(s) 205
Fsp4HI GCNGC 1 cut(s) 408
FspBI CTAG 2 cut(s) 68, 434
GluI GCNGC 1 cut(s) 408
GsuI CTGGAG 2 cut(s) 167, 208
HaeIII GGCC 1 cut(s) 373
HapII CCGG 1 cut(s) 450
HgaI GACGC 1 cut(s) 448
Hin1II CATG 1 cut(s) 382
HincII GTYRAC 3 cut(s) 96, 114, 463
HindII GTYRAC 3 cut(s) 96, 114, 463
HinfI GANTC 2 cut(s) 194, 430
HpaI GTTAAC 1 cut(s) 114
HpaII CCGG 1 cut(s) 450
HphI GGTGA 1 cut(s) 189
Hpy166II GTNNAC 5 cut(s) 58, 96, 114, 353, 463
Hpy188I TCNGA 1 cut(s) 193
Hpy8I GTNNAC 5 cut(s) 58, 96, 114, 353, 463
HpyAV CCTTC 1 cut(s) 384
HpyCH4III ACNGT 1 cut(s) 397
HpyCH4IV ACGT 1 cut(s) 282
HpyCH4V TGCA 2 cut(s) 130, 419
HpyF10VI GCNNNNNNNGC 1 cut(s) 416
HpyF3I CTNAG 2 cut(s) 272, 293
HpySE526I ACGT 1 cut(s) 282
Hsp92II CATG 1 cut(s) 382
KspAI GTTAAC 1 cut(s) 114
Kzo9I GATC 2 cut(s) 253, 493
LpnPI CCDG 6 cut(s) 197, 211, 238, 372, 463, 489
Lsp1109I GCAGC 1 cut(s) 419
MaeI CTAG 2 cut(s) 68, 434
MaeII ACGT 1 cut(s) 282
MalI GATC 2 cut(s) 255, 495
MboI GATC 2 cut(s) 253, 493
MboII GAAGA 3 cut(s) 10, 131, 311
MflI RGATCY 1 cut(s) 493
MluCI AATT 4 cut(s) 136, 171, 204, 267
MmeI TCCRAC 1 cut(s) 462
MnlI CCTC 4 cut(s) 191, 232, 350, 480
Mph1103I ATGCAT 1 cut(s) 421
MseI TTAA 3 cut(s) 113, 210, 236
MspA1I CMGCKG 1 cut(s) 161
MspI CCGG 1 cut(s) 450
MspR9I CCNGG 2 cut(s) 226, 450
Mva1269I GAATGC 2 cut(s) 128, 421
MvaI CCWGG 1 cut(s) 226
MvnI CGCG 1 cut(s) 459
MwoI GCNNNNNNNGC 1 cut(s) 416
NciI CCSGG 1 cut(s) 450
NdeII GATC 2 cut(s) 253, 493
NlaIII CATG 1 cut(s) 382
NsiI ATGCAT 1 cut(s) 421
PctI GAATGC 2 cut(s) 128, 421
PfeI GAWTC 2 cut(s) 194, 430
PkrI GCNGC 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 224
PspGI CCWGG 1 cut(s) 224
PsuI RGATCY 1 cut(s) 493
PvuII CAGCTG 1 cut(s) 161
RsaI GTAC 2 cut(s) 277, 446
RsaNI GTAC 2 cut(s) 276, 445
SaqAI TTAA 3 cut(s) 113, 210, 236
SatI GCNGC 1 cut(s) 408
Sau3AI GATC 2 cut(s) 253, 493
ScaI AGTACT 1 cut(s) 277
ScrFI CCNGG 2 cut(s) 226, 450
SetI ASST 6 cut(s) 63, 163, 189, 285, 294, 342
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 4 cut(s) 136, 171, 204, 267
SspI AATATT 1 cut(s) 318
SspMI CTAG 2 cut(s) 68, 434
StyD4I CCNGG 2 cut(s) 224, 448
StyI CCWWGG 1 cut(s) 61
TaaI ACNGT 1 cut(s) 397
TaiI ACGT 1 cut(s) 285
TaqI TCGA 1 cut(s) 256
TasI AATT 4 cut(s) 136, 171, 204, 267
TatI WGTACW 1 cut(s) 275
TfiI GAWTC 2 cut(s) 194, 430
Tru1I TTAA 3 cut(s) 113, 210, 236
Tru9I TTAA 3 cut(s) 113, 210, 236
TseI GCWGC 1 cut(s) 407
TspDTI ATGAA 3 cut(s) 17, 327, 428
XapI RAATTY 1 cut(s) 267
XspI CTAG 2 cut(s) 68, 434
ZrmI AGTACT 1 cut(s) 277
Zsp2I ATGCAT 1 cut(s) 421
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.